10 results about "Dosage effect" patented technology

The invention belongs to the technical field of perioperative anticoagulation management, and particularly relates to a heparin dose-effect relationship modeling method, a heparin dose-effect relationship prediction system and a storage medium. The modeling method comprises the following steps: inputting clinical data from a single patient, wherein the clinical data comprises the administration dosage of heparin and a corresponding ACT measurement value; fitting different models suitable for the heparin dose-effect relationship based on the clinical data, and selecting an optimal model through cross validation; and re-training the optimal model by adopting all clinical data of a single patient to obtain the optimal model actually used for deployment. The invention also provides a system for predicting the heparin dose-effect relationship by using the model. Compared with a single model for predicting the heparin dosage-effect relationship, the method has higher accuracy, is beneficial to accurately controlling the heparin dosage of a patient, and has a very good application prospect in anticoagulation management in the perioperative period.

The invention discloses a screening and dosage determining method of a grape heat resistance inducer, and belongs to the technical field of plant stress resistance physiology and cultivation. The method comprises the following steps: soaking grape leaf wafers in inducers with different concentrations, and carrying out heat treatment under a plurality of temperature gradients; determining a chlorophyll OJIP rapid fluorescence parameter and constructing a heat resistance comprehensive index, and further calculating a normalized relative heat resistance improvement value; fitting a quantitative relation with the concentration by using a single-peak dose-effect model containing a high-dose inhibition item; statistical inference is carried out by adopting Bootstrap resampling, and a stable and effective recommended application concentration in a wide temperature range is finally determined by analyzing a common intersection or a statistical median of effective concentration intervals under multiple temperatures. The method realizes indoor rapid and quantitative screening, can realize quantitative comparison of induction intensity and dose sensitivity of different inducers under a unified evaluation system, and can be used for identifying heat resistance of grapes.

Disclosed are a method for screening a riboswitch capable of specifically recognizing doxycycline as well as rational engineering and application of the riboswitch, and belongs to the field of gene expression regulation. The present disclosure provides a doxycycline specific riboswitch, and establishes a high-throughput screening method based on flow cytometry, and obtains the doxycycline riboswitch with further improved activation fold by way of computer-aided prediction of binding sites of the riboswitch and calculation of mutation sites. According to a dose-response curve, the riboswitch shows linear correlation at 40-100 μg / L, and meanwhile, a whole-cell sensor constructed is low in price, high in stability and simple to operate, and can be used for on-site rapid detection.

The invention relates to a gene dose effect target spot for improving rapamycin resistance and application of the gene dose effect target spot. A target spot gene is YCL039W and / or YCL037C, or human WDR26 and / or LARP1; the YCL039W and / or YCL037C are / is overexpressed in the saccharomycetes, so that the rapamycin resistance of the saccharomycetes can be improved, and the constructed recombinant function can better grow under the pressure condition of the rapamycin; and the sensitivity of the rapamycin can be improved by knocking down the WDR26 and / or the LARP1 in human cells, so that the application of the rapamycin drug resistance in clinical treatment is widened.

The invention discloses a phase inverter capable of simultaneously resisting total dose and single event effect, which adopts the structural design that a main phase inverter and an auxiliary phase inverter are combined, and the main phase inverter is composed of two PMOS (P-channel Metal Oxide Semiconductor) tubes connected in series and an NMOS (N-channel Metal Oxide Semiconductor) tube. On the basis of a mode that an auxiliary phase inverter provides a level compensation signal at the NMOS source end of a main phase inverter, the high level output by the phase inverter under the total dose effect is prevented from being reduced, and transient upset of the output level of the phase inverter from high to low under the single event effect of an NMOS tube is avoided; on the basis of a double PMOS structure design mode, transient upset of the output level of the inverter from low to high caused by conduction of a PMOS tube under a single event effect is avoided. And finally, stable output of the inverter under the total dose and single-particle system effect can be realized.

The invention provides a Col4a5 + / -mouse immortalized podocyte line, and the preservation number of the Col4a5 + / -mouse immortalized podocyte line is CGMCC (China General Microbiological Culture Collection Center) NO.46563. The cell line has relatively strong stability guarantee and can be subjected to continuous passage for 30 generations. The Col4a5 < + / -> mouse immortalized podocyte line corresponds to the phenotype of a female XLAS carrier with a light disease degree, can be used for precise pathological modeling, realizes COL4A5 gene dose effect simulation, and can be used as a cell model for screening a treatment drug or a diagnosis drug for hereditary nephropathy. The cell line is subjected to immortalization treatment, can be massively cultured and subcultured, has relatively simple culture requirements, can obtain a large number of experimental cells in a short time, has a short total experimental period, is suitable for popularization, and overcomes the technical problems of difficulty in constructing a single allele mutation (Col4a5 + / -) cell line, low transfection efficiency, allele loss and the like in the prior art.

The invention discloses GhRALF4L and GhRALF30L gene clusters and an application of the GhRALF4L and GhRALF30L gene clusters in creating a high-temperature male sterile line of cotton. The invention provides two RALF gene clusters GhRALF4L and GhRALF30L related to the high-sensitivity male sterility character of cotton, the two RALF gene clusters comprise 28 related genes in total, and the coding sequences of the genes are respectively shown as SEQ ID NO.1-28. The two gene clusters regulate the high-temperature fertility of drugs and pollen of upland cotton through a dose effect, and a gene editing mutant with higher editing efficiency is more sensitive to high-temperature stress, i.e., the more serious function loss of GhRALF4L and GhRALF30L causes the more serious high-temperature male sterility. According to the gene editing mutant created on the basis of the discovery, the sterility character has the definite high-temperature induction characteristic, that is, the mutant is normal in fertility under the normal-temperature condition and shows abortion only under the high-temperature condition.

This invention discloses the application of Ikzf3 in peripheral blood leukocytes in calculating high-energy neutron radiation dose, belonging to the field of biodetection technology. This invention focuses on the biological effects and damage mechanisms of neutron radiation, screening potential biomarkers under dose gradients. The LD50 and LD100 of neutron radiation in mice were determined to be 8 Gy and 12 Gy, respectively. The degree of damage to radiation-sensitive organs such as the spleen and intestines increased with the dose gradient. The number of lymphocytes in the blood system decreased significantly with dose, which can serve as an indicator for assessing radiation damage. Cytokines such as IL-6 and IL-10 were significantly upregulated, suggesting inflammatory and immune regulatory mechanisms. Finally, Ikzf3 was screened as a potential key gene, and its expression level changed significantly before and after irradiation, exhibiting extremely high sensitivity. This method, by detecting the expression level of Ikzf3 in peripheral blood leukocytes and substituting it into a dose-response function, can achieve rapid and objective estimation of neutron radiation dose, possessing clinical translational value.

The invention provides a Col4a5- / -mouse immortalized podocyte line, and the preservation number of the Col4a5- / -mouse immortalized podocyte line is CGMCC (China General Microbiological Culture Collection Center) NO.46563. The Col4a5- / -mouse immortalized podocyte line has relatively strong stability The Col4a5- / -mouse immortalized podocyte line corresponds to the phenotype of a female XLAS carrier with a relatively serious illness degree, can be used for precise pathological modeling, realizes COL4A5 gene dose effect simulation, and can be used as a cell model for screening a treatment drug or a diagnosis drug for hereditary nephropathy. The cell line is subjected to immortalization treatment and can be massively cultured and subcultured, culture requirements are relatively simple, a large number of experimental cells can be obtained in a short time, the total experimental period is short, and the cell line is suitable for popularization.

The invention relates to a gene dose effect target for improving the tolerance of yeast and application, and particularly provides a group of gene combinations YCL051W-YCL047C capable of enhancing the heat resistance of the yeast, and the gene combinations include YCL051W, YCL050C, YCL049C, YCL048W and YCL047C. A yeast engineering bacterium with heat resistance can be constructed by overexpressing a gene combination YCL051W-YCL047C in a yeast strain, the target combination is suitable for various different types of industrial strains, and the modified strain shows enhanced heat resistance and can accelerate microbial metabolism, improve the production efficiency and remarkably reduce the industrial fermentation cost; meanwhile, the production process efficiency and stability are improved, and the contamination risk is reduced.