Method for detecting toxicity influence of stomach toxicity pesticides or transgene insecticidal protein to Propylaea japonica

The invention relates to a technology of Ladybug moriformis and a measuring method, which is applied in the directions of measuring devices, instruments, scientific instruments and the like to achieve the effect of simple and feasible method.

Active Publication Date: 2014-03-05
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Method for detecting toxicity influence of stomach toxicity pesticides or transgene insecticidal protein to Propylaea japonica
  • Method for detecting toxicity influence of stomach toxicity pesticides or transgene insecticidal protein to Propylaea japonica
  • Method for detecting toxicity influence of stomach toxicity pesticides or transgene insecticidal protein to Propylaea japonica

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[0018] The invention provides a simple and sensitive assay method for detecting the direct toxicity effect of gastric toxic insecticides or transgenic insecticidal proteins on Coccinea rubra. In this method, the established pollen-based feed is directly fed to the lady beetle by mixing the insecticide or transgenic insecticidal protein uniformly, and the feed mixed with the protease inhibitor E-64 (225 μg E-64 / g pollen) as the positive control, and the feed without any insecticidal compounds was used as the negative control. By comparing the survival rate, developmental period, body weight and egg production of the treatment group and the control group, the important life indicators such as ladybugs' survival rate, developmental duration, body weight and egg production were evaluated. Potential toxicity. The biological response of E-64 to different concentrations of E-64 was determined by this method. The different life parameters of E-64 showed obvious dose-response relation...

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Abstract

The invention provides a method for detecting toxicity influence of stomach toxicity pesticides or transgene insecticidal protein to Propylaea japonica. The method is characterized in that the pesticides or transgene insecticidal protein can be uniformly mixed in a feed which takes pollen as a base to directly feed in Propylaea japonica, taking a feed (225mumg E-64/g of pollen) mixed with a protease inhibitor E-64 as a positive control, taking the feed without mix of any insecticidal compound as a negative control, and evaluating the latent toxicity of the insecticidal compound by comparing the important life index of Propylaea japonica such as survival rate, growth stage, body weight and oviposition amount of a processing group and a control group. According to the invention, the method is employed to detect the biology reaction of Propylaea japonica to E-64 with different concentration, different life parameters of Propylaea japonica display obvious a dosage-effect relation with the changer of E-64 concentration, so that validity and sensitivity of the method can be proved.

Description

technical field [0001] The invention relates to a method for measuring the toxicity of gastrotoxic insecticides or transgenic insecticidal proteins on Coccinella gracilis. Background technique [0002] Predatory lady beetles (Coleoptera: Coccinellidae) are common and important natural enemy insects in field ecosystems of various crops such as cotton, corn, rice, and vegetables, and play an important role in crop pest control. Therefore, this kind of natural enemy insects has always attracted much attention in the research on the environmental safety effects of pesticides and transgenic plants. Especially in recent years, the widespread planting of transgenic insect-resistant crops, which may have a negative impact on natural enemy insects, has attracted extensive attention of relevant scientists. The potential impact of genetically modified insect-resistant crops such as Bt crops on ladybird natural enemies has also become an important part of the environmental safety asses...

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Application Information

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IPC IPC(8): A23K1/18A23K1/14A23K1/175G01N33/00
Inventor 李云河张小洁彭于发
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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