Novel diterpenoid compound for treating liver cancer
A technology for the treatment of liver cancer, compounds, applied in the field of medicine, can solve problems such as susceptibility to disease, aggravation of disease, and weak antiviral ability
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Embodiment 1
[0024] Example 1: Compound (I) Separation Preparation and Structure Confirmation
[0025] Sources of medicinal materials and reagents: ethanol, petroleum ether, ethyl acetate, n-butanol, and dichloromethane are analytically pure, purchased from Shanghai Lingfeng Chemical Reagent Co., Ltd. Methanol, analytically pure, are purchased from Jiangsu Hanbang Chemical Reagent Co., Ltd. The dried whole herb of the urophyll fragrant tea vegetable was purchased from the Chinese herbal medicine market in Bozhou, Anhui, and the place of origin is Guangdong.
[0026] Preparation method: (a) Grind the dried whole herb (8kg) of C. urophylla, heat-reflux extraction with 75% ethanol (25L x 3 times), combine the extracts, concentrate until no alcohol smell (6L), and successively use Petroleum ether (6L×3 times), ethyl acetate (6L×3 times) and water-saturated n-butanol (6L×3 times) were extracted and concentrated to obtain petroleum ether extract and ethyl acetate extract (315g) respectively and...
Embodiment 2
[0028] Embodiment 2: compound (I) pharmacological action test
[0029] 1. Materials and Instruments
[0030] HepG2 human liver cancer cell line was purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. The L02 normal human liver cell line was donated by the Liver Cancer Institute, Zhongshan Hospital, Fudan University. Compound (I) is self-made, and the HPLC normalized purity is greater than 98%. RPMI-1640 medium and trypsin were purchased from Gibco. Standard calf serum was purchased from Biowest. 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT), dimethylsulfoxide (DMSO), trypan blue (TrypanBLue), dichlorofluorescence Diacetate diacetate (DCFH-DA) was purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd. Other commonly used reagents were of analytical grade.
[0031] Microbalance (Mettler-Toledo, Switzerland, METTLRERAE2000). Ordinary desktop centrifuge (Shanghai Anting Scientific Instrument Factory). Digital...
Embodiment 3
[0056] Preparation of tablets: firstly prepare compound (I) according to the method of Example 1, and utilize organic acids such as tartaric acid, or citric acid, formic acid or oxalic acid, etc., inorganic acids such as hydrochloric acid or sulfuric acid or phosphoric acid to make salts, according to its The weight ratio of the excipient to the excipient is 1:9, and the excipient is granulated and compressed into tablets.
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