27results about How to "No toxicity" patented technology

The present application relates to the technical field of biological medicine, and discloses inhalable aptamer modified molybdenum disulfide nanoflower compound and application thereof.The compound comprises a molybdenum disulfide nanoflower core, a dendritic polylysine modification layer attached to the surface of the molybdenum disulfide nanoflower, doxorubicin loaded on the molybdenum disulfide nanoflower and / or the dendritic polylysine modification layer, and AS1411 aptamer combined on the outer surface of the dendritic polylysine modification layer by electrostatic assembly.The compound can be delivered to a lung lesion by atomization inhalation or aerosol inhalation, and undergoes responsive structural change under the action of hydrogen peroxide in a tumor microenvironment, generates active oxygen, and promotes the release of doxorubicin, so as to realize the synergistic antitumor effect of chemotherapy and chemical dynamic therapy.The compound has good colloidal stability, drug loading capacity, active targeting ability and lung local delivery potential, and can be used for preparing inhalation preparations or local administration preparations for treating lung cancer.

This invention proposes a combined inhibitor for separating bastnaesite and monazite, along with its method and application. The raw materials for the combined inhibitor include small organic molecules and microbial polysaccharides; the small organic molecules include one or more of oxalic acid, malic acid, tartaric acid, lactic acid, succinic acid, and citric acid; the microbial polysaccharides include one or more of currant gum, guar gum, xanthan gum, and gellan gum. This invention achieves highly selective inhibition of monazite on the mineral surface through the synergistic adsorption and action of the inhibitor, with small organic molecules and microbial polysaccharides as the main components, thereby efficiently separating bastnaesite under mild flotation conditions. It offers advantages such as good selectivity, strong action, low reagent cost, and environmental friendliness.

The application provides a kind of mitochondrial function optimization additive for cell culture, preparation method and application, belong to cell culture technical field.The additive includes core active ingredient, auxiliary component and carrier, and the core active ingredient includes D-Arg-Cha-Lys-Phe-NH2 Polypeptide, ubiquinone-10 phosphate, reduced glutathione and alpha-lipoic acid.The application forms a multi-target core active system through the synergy of the four, realizes the triple synergistic protection of stabilizing mitochondrial cardiolipin, maintaining mitochondrial morphology and membrane potential, and reducing mitochondrial ROS, which can significantly improve cell culture quality.When applied, the mitochondrial cardiolipin content can be increased by more than 50%, the mitochondrial abnormality ratio can be reduced to less than 12%, the membrane potential positive cell ratio can be increased to more than 86%, the mitochondrial ROS level can be reduced by more than 40%, and the cell proliferation rate can be increased by more than 25%, which is suitable for in vitro culture of various eukaryotic cells, and the preparation process is simple and has no cytotoxicity.

This application relates to the field of carbon source materials, and in particular to a composite carbon source for enhancing the denitrification capacity of activated sludge, its preparation method, and its application. The composite carbon source for enhancing the denitrification capacity of activated sludge, by weight, comprises: 45-60 parts of fast-acting carbon source, 10-15 parts of long-acting carbon source, 9-16 parts of synergist, 0.2-0.5 parts of activator, 4-8 parts of composite functional agent, and 20-25 parts of deionized water. This composite carbon source effectively promotes the denitrification start-up speed, provides a stable and continuous carbon source supply, effectively avoids the defects of traditional carbon sources such as excessive supply in the early stage and insufficient supply in the later stage, and ensures efficient operation of the entire denitrification process. It is safe and convenient to use, non-toxic, and poses no risk of combustion or explosion. Storage and addition are simple, significantly reducing on-site operational safety risks. The carbon release process is stable and controllable, and multiple slow-release mechanisms enable flexible regulation of the carbon source release rate to match the denitrification requirements under different operating conditions.

The invention relates to the technical field of veterinary drugs, and particularly provides a polynucleotide molecule composition for preventing cat infectious peritonitis, a chimeric polynucleotide molecule and an mRNA vaccine. In order to overcome the ADE effect caused by S protein full-length antigen in the prior art, FIPV N protein without ADE risk is adopted and is combined with screened S protein specific T cell and B cell epitopes. Core schemes include two classes: one is a separate polynucleotide composition comprising epitope peptides encoding N and S proteins; and 2, a single chimeric polynucleotide encoding N protein and S protein epitope peptides. The polynucleotides are optimized by codons and encapsulated in lipid nanoparticles (LNP) to prepare vaccines. Experiments prove that the vaccine can synergistically stimulate powerful immune response, the survival rate of immune cats is remarkably increased to 80%-100% from standard 40% on the premise that ADE is not caused, and FIP is effectively prevented.

The invention discloses a plant immune induced resistance protein Ps131903 and application thereof. The amino acid sequence of the immune induced resistance protein Ps131903 is shown as SEQ ID NO.2. The protein Ps131903 can reduce the harm of phytophthora sojae and pythium ultimum to soybeans, reduce the harm of phytophthora capsici to chilies and tomatoes and reduce the harm of pyricularia grisea to rice. The compound has the advantages of low use concentration, quick effect and broad spectrum, and provides a new way for improving the plant resistance, thereby having wide application prospects in agricultural production.

The application discloses an affinity peptide targeting human CD81 protein and application thereof. The amino acid sequence of the affinity peptide provided by the application is shown as SEQ ID NO: 7. The affinity peptide provided by the application has high affinity and high selectivity with a small loop fragment of an extracellular CD81 protein, can recognize and combine with the CD81 protein in free and living cells, can be used for high-sensitivity detection and in-situ imaging of the CD81 protein, and can be further used as a selective inhibitor of a migration process of tumor cells expressing the CD81 protein. The affinity peptide provided by the application can specifically target and recognize the CD81 protein, is a specific probe for analysis and detection of the CD81 protein and functional research, and has application prospects in detection of diseases such as malignant tumors and development of targeted drugs. The application has important application value.

The application relates to the technical field of high-molecular fiber sensing, in particular to a high-toughness TPU / ANF / inorganic nanoparticle composite nanometer friction positive electrode material and a preparation method thereof. The preparation method takes TPU as a base main material, does not depend on a chemical cross-linking method, and realizes flexible adaptation of a complex deformation scene by virtue of excellent stretchable deformation performance and flexible characteristics of the TPU. Meanwhile, aramid nanofiber (ANF) is introduced as a reinforcing phase, the high-strength and high-modulus characteristics of the aramid nanofiber are utilized to improve the stress and strain capacity and mechanical stability of the base, the aramid nanofiber can lock the molecular structure in the TPU, and the aramid nanofiber can inhibit significant shrinkage of the material when the temperature is increased, so that the aramid nanofiber has the dual functions of mechanical enhancement and size stability, and the reliability of a generator is ensured.

The utility model relates to the technical field of endoscopes, and discloses an endoscope insertion tube and an endoscope, and the endoscope insertion tube comprises a metal elastic layer, a metal braid layer and a self-repairing layer. The self-repairing layer comprises a self-repairing resin layer and a self-repairing coating layer; the self-repairing resin layer is arranged outside the metal braid layer, the self-repairing coating layer is arranged outside the self-repairing resin layer, and the material of the self-repairing coating layer comprises a coating with self-repairing capability. The outer surface of the endoscope insertion tube with the structure is the self-repairing layer, when the outer surface of the insertion tube is scratched or damaged due to improper use of an operator, the self-repairing layer can automatically repair the outer surface of the insertion tube under the conditions of heating, illumination or radiation and the like, the outer surface of the insertion tube is convenient to repair, the repairing cost is low, and the service life of the insertion tube is prolonged. The service life of the insertion pipe can be effectively prolonged; when the outer surface of the insertion tube is damaged, the insertion tube does not need to be replaced, so that the repair and maintenance cost of medical equipment can be reduced.

The application belongs to the technical field of biological medicine, and particularly relates to a dipicolinate ester derivative and application thereof. The dipicolinate ester derivative has broad-spectrum antitumor activity, can significantly inhibit the proliferation activity of lung cancer cells, pancreatic cancer cells and prostate cancer cells, and has more excellent inhibitory activity on lung cancer cells, and can significantly inhibit the growth of mouse subcutaneous small cell lung cancer transplanted tumors. In addition, the dipicolinate ester derivative has no toxic effect on normal cells of the body, can be applied to preparation of anticancer drugs, and has broad application prospects in the aspect of anticancer.

The intranasal administration of 1, 3, 5 (10), 16-estratetraen-3-yl acetate may be used to improve mental motor and / or cognitive performance in mental fatigue subjects.

This invention discloses the application of a nucleoside phosphoramide ester derivative, CL-13, in the preparation of antiviral drugs. The virus is selected from flaviviruses or enteroviruses, and the structure of CL-13 is shown below. This invention discovers a novel use of compound CL-13 in antiviral applications. Experimental results show that CL-13 has significant inhibitory effects on Zika virus (ZIKV), dengue virus type 2 (DENV2), Japanese encephalitis virus (JEV), enterovirus 71 (EV71), and tick-borne encephalitis virus (TBEV), with the inhibitory effect being concentration-dependent and exhibiting a broad antiviral spectrum. Simultaneously, CL-13 possesses strong antiviral activity and shows no significant cytotoxicity within the effective antiviral concentration range, exhibiting a high therapeutic index, good safety profile, and excellent drug development potential. Therefore, CL-13 combines broad-spectrum, high-efficiency, and low-toxicity characteristics, making it suitable for preparing drugs to prevent or treat diseases caused by the aforementioned viral infections, and has significant application prospects.

The invention relates to the technical field of material surface treatment, in particular to a salt bath chromium plating method for the surface of diamond powder. By adopting a composite chloride system with magnesium chloride as a main component and alkali metal chloride as an auxiliary component, a low-melting-point co-melt can be formed in the salt bath chromium plating process, the melting point can be reduced to 700 DEG C or below, the process temperature of salt bath chromium plating is remarkably reduced, meanwhile, the molten salt flowability is optimized, and the salt bath chromium plating quality is improved. It is ensured that chromium atoms are evenly diffused in the fused salt and chemically react with carbon atoms on the surface of the diamond to generate a uniform and compact chromium carbide layer, and heat damage to the diamond is effectively avoided; the chlorate powder is low in cost, easy to obtain and free of toxicity, harmful substances cannot be generated in the salt bath chromium plating process, the environmental protection property is good, the bottlenecks of high temperature, high consumption and uneven plating of traditional salt bath chromium plating are broken through, a low-cost and high-reliability surface modification technology is provided for the diamond / metal composite material, and the application prospect is wide. And the large-scale application of the diamond in the fields of electronic packaging and thermal management is directly promoted.

This invention discloses a method for cryopreservation of donkey semen using antifreeze peptides, belonging to the field of animal reproductive technology. This invention involves adding 8-polyproline (L-Pro) to the donkey semen cryopreservation solution. 8 5mM and 8-polyarginine (L-Arg) 8 5mM, and reduce the glycerol concentration to 1%. L-Pro 8 and L-Arg 8 Possessing a PPII double helix structure, it can effectively inhibit ice crystal growth and protect cell membrane structure. This invention can significantly improve the motility of frozen donkey sperm, protect intracellular structures such as mitochondria and DNA, and increase the conception rate of frozen sperm insemination, thus having significant application value.

The invention provides application of p-hydroxycinnamic acid in preparation of a medicine for preventing and treating noise-induced deafness. The invention has the advantages that the p-hydroxycinnamic acid has the advantages of strong pertinence, high safety, convenience in medication, good patient compliance and the like, not only effectively overcomes the defects of the existing noise-induced hearing loss treatment medicines, but also provides a brand-new preventive treatment thought; important clinical popularization significance and industrial application prospects are realized.

The application belongs to the technical field of medicine, and particularly relates to a traditional Chinese medicine composition for treating bronchiectasis. The traditional Chinese medicine composition is composed of the following traditional Chinese medicines: rhizoma phragmitis, raw semyen of job's tears, winter melon seed, persicae semen, platycodon grandiflorum, semen balsamni, dioscorea, prepared rhizome of rehmannia, radix codonopsis, pinellia ternata, poria cocos and pericarpium citri reticulatae. The traditional Chinese medicine composition is combined together, and through scientific compounding, each medicine plays a synergistic effect, realizes a significant treatment effect on bronchiectasis, has a high clinical application value, and has a wide application prospect.

This invention belongs to the field of gold extraction agent technology, specifically relating to a method for preparing a gold extraction agent. The invention provides a method for preparing a gold extraction agent, comprising the following steps: mixing sodium cyanate and iron powder, and carrying out a melt catalytic reaction to obtain the gold extraction agent. This invention uses iron powder as a catalyst, which can effectively promote the polymerization reaction of sodium cyanate. During the catalytic polymerization process, iron atoms can break the carbon-nitrogen triple bond in sodium cyanate into a carbon-nitrogen double bond, with one end of the carbon-nitrogen double bond linked to a sodium atom. The obtained gold extraction agent does not contain free cyanide ions and is therefore non-toxic. In application, the sodium in the obtained gold extraction agent can displace gold in gold ore, thus playing a role in gold refining. Test results from the embodiments show that the gold extraction efficiency of the gold extraction agent provided by this invention is comparable to that of sodium cyanide, and it is safe, environmentally friendly, and non-toxic.

This invention belongs to the field of ultra-high molecular weight polyethylene (UHMWPE) fiber modification technology, specifically relating to a hydrocarbon-linked boron nitride-modified UHMWPE fiber with high creep resistance and its preparation method. The fiber comprises an UHMWPE matrix and hydrocarbon-linked grafted boron nitride dispersed therein; the hydrocarbon-linked grafted boron nitride is obtained by grafting ω-heptadecenylphosphonic acid onto the surface of hydroxylated nano-hexagonal boron nitride, exhibiting a dual physical crosslinking mechanism. The preparation method includes preparing hydroxylated nano-hexagonal boron nitride, preparing hydrocarbon-linked grafted boron nitride, and preparing high creep-resistant UHMWPE fiber. The method of this invention can significantly improve the creep resistance of the fiber, exhibiting mechanical stability, with a creep rate ≤1.5% after 1000 hours of stress loading at 200 MPa at room temperature. This invention features a simple process, strong adaptability, mild and controllable process parameters, low equipment modification costs, and is easy to industrialize.

This invention discloses a multifunctional chemical concentrate for urban greenbelts and its preparation method, belonging to the field of greening maintenance technology. The concentrate comprises 10%-30% functional film-forming agent, 5%-20% flame retardant and smoke suppressant, 3%-15% organic decay promoter, 1%-8% surfactant, 0.5%-3% pH adjuster, and the balance deionized water. This invention integrates soil stabilization and water retention, flame retardancy and smoke suppression, and decay promotion and soil improvement functions. The formula can be adjusted according to the climate of northern and southern regions and plant types. It is convenient to construct, low in cost, and environmentally friendly, suitable for the maintenance and management of various types of urban greenbelts.

The application discloses a compound with neuroprotective activity, a preparation method and application. A new compound of diketopiperazine alkaloids is separated from a fermentation product of Aspergillus, the compound is stable in performance, has no cytotoxicity, and has significant neuroprotective activity. The compound can improve the cell survival rate of a nerve injury in vitro model at a lower concentration, inhibit the generation of active oxygen and malondialdehyde, and obviously increase the expression amount of superoxide dismutase, and has the effects of antioxidation and anti-nerve injury, and has a wide application prospect in the preparation of medicines for preventing and treating neurodegenerative diseases.

The present application relates to the technical field of carbon quantum dots, and particularly relates to a kind of polymer fluorescent carbon dots with PBAT as carbon source, its preparation method and application in detecting iron ion.The preparation method is as follows, using air oxidation of poly (butylene adipate-co-terephthalate) (PBAT) and terephthalic acid butylene glycol ester, then hydrothermal treatment in H2O2 aqueous solution to prepare CDs, PBAT is placed in open ceramic crucible and air oxidation, forming dark brown oxidation product.Then, the product is transferred to the reaction kettle containing H2O2 solution.After hydrothermal treatment, CDs are brown dispersion.CDs dispersion is centrifuged, dialyzed, freeze-dried, and finally CDs powder is obtained.The carbon quantum dots prepared by the present application are synthesized by hydrothermal method in one step, have stable fluorescent properties, and are non-toxic.The high-efficiency fluorescent carbon quantum dots of the present application can be used as fluorescent probe for quantitative measurement of iron ion.

The application provides a kit for detecting AChR blocking antibodies, a detection method and application, and belongs to the technical field of medical detection. The application provides a compound for detecting AChR blocking antibodies instead of alpha-BGT. Compared with alpha-BGT, the compound is non-toxic, cheap, easy to obtain, and can still detect AChR blocking antibodies after high-temperature treatment. The application also provides a method for detecting AChR blocking antibodies by using the compound. The compound does not need to be labeled with radioactive iodine or other substances, thereby reducing the cost, avoiding the influence of radioactive substances on the environment and the harm to experimenters, and being green and environmentally friendly. The compound can also be incubated with a serum sample at the same time, thereby shortening the detection time and improving the detection efficiency.

The application relates to the technical field of anti-counterfeiting materials, in particular to a bio-based anti-counterfeiting filter paper and a preparation method thereof. The application provides a bio-based anti-counterfeiting filter paper which comprises a base material and an anti-counterfeiting dye layer compounded on the base material; the anti-counterfeiting dye layer is prepared from anti-counterfeiting dyes; the anti-counterfeiting dyes are prepared from raw materials including isocyanate, polyethylene glycol, 2,2-dimethylol propionic acid, ethylenediamine and hematoxylin. The bio-based anti-counterfeiting filter paper provided by the application is doped with special anti-counterfeiting dyes, provides unique spectral reflection information, and has the advantages of being difficult to replicate, non-toxic and long service life. Compared with the anti-counterfeiting filter paper currently in circulation, the bio-based anti-counterfeiting filter paper provided by the application can reduce the inflow of fake and inferior products into the market, reduce the use risk of customers, and protect the legal rights and interests of customers.

The application discloses a PHA water-based coating paper pulp molding seedling raising container with functions of air preservation, root guidance and disintegration promotion, and a preparation method and application thereof, and belongs to the technical field of green agriculture and biomaterials. The application innovatively constructs an air preservation, root guidance and disintegration promotion synergistic zone, and solves the pain point that traditional paper-based containers are difficult to balance water resistance and air permeability by using differential coating technology. Compared with the traditional scheme which needs to greatly increase the coating thickness to maintain the wet strength, the application realizes better wet stability and root zone air permeability by synergistic effect of structure and material with only a very low coating amount, and gives the container the functions of intelligent root guidance penetration and directional cracking after transplanting. The product supports pot-free planting and significantly reduces the comprehensive seedling raising cost, and is in line with the future development direction of full biodegradation.

This invention belongs to the field of pharmaceutical technology and discloses the inhibitory effects and mechanisms of action of sclerotinib on lung cancer cells, cervical cancer cells, and ovarian cancer cells. Compared with the currently available broad-spectrum antitumor drug fluorouracil, sclerotinib exhibits superior antitumor properties. In-depth research revealed that sclerotinib acts on the novel protein STAT3 in cancer treatment, inhibiting STAT3 phosphorylation and preventing phosphonate-modified protein dimers from entering the cell nucleus to initiate transcription. Furthermore, sclerotinib inhibits the downstream anti-apoptotic protein BCL-2 of STAT3, synergistically inhibiting tumor cell proliferation. In this study, based on the half-maximal inhibitory concentration (IC50) results of sclerotinib against lung cancer cells A549, cervical cancer cells HeLa, and ovarian cancer cells A2780, we directly verified the anticancer target of sclerotinib through experiments, elucidated its anticancer mechanism, and provided theoretical and experimental basis for its entry into preclinical and clinical trials.

This invention discloses a fluorine-nitrogen co-doped carbon dot, its preparation method, and its applications. The fluorine-nitrogen co-doped carbon dot is a nanoparticle constructed using a one-step hydrothermal synthesis method with fluorine-containing and nitrogen-containing precursors. The nanoparticle is spherical with a particle size of 5 nm, and its surface is rich in carboxyl, amino, and hydroxyl functional groups. Its fluorescence quantum yield is not less than 20%, and its emission wavelength is in the 500-550 nm range. It has the characteristic of targeting the H3K36me2 histone modification in tumor cell nuclei, and can achieve specific labeling of tumor cells within 5 minutes. The enrichment intensity in tumor tissue is more than 2.72 times that in normal tissue. The fluorine-nitrogen co-doped carbon dot of this invention is expected to serve as a novel fluorescent labeling probe for tumor identification and imaging, contributing to the early and accurate diagnosis of malignant tumors. It has multiple advantages such as speed, accuracy, safety, and visualization, and has broad application prospects in tumor pathology identification and intraoperative navigation.

The application discloses a method for reducing the content of phthalate plasticizer in natural plant extract, which comprises the following steps: mixing natural plant extract powder and an aqueous solution of ethanol with a volume concentration of 95% or anhydrous ethanol according to a ratio of 1g:2-10mL, stirring and dissolving under the condition of 20-80 DEG C, then adding water with a mass of 2-10 times that of the natural plant extract, mixing, standing for 10-20min, and centrifuging or filtering; and washing the obtained solid with pure water, drying at 40-80 DEG C, and obtaining the natural plant extract with low content of phthalate plasticizer. The method is simple, non-toxic, and free of discharge of organic pollutants. The method can effectively reduce the content of phthalate plasticizer in the natural plant extract, so that the content of the plasticizer reaches below the limited value, and the content of effective components also meets the quantitative standard, and the plasticizer removal rate is greater than or equal to 82%.