245results about How to "No toxicity" patented technology

The invention discloses an anti-EV71 virus activity buthus martensii toxin polypeptide as well as a preparation method and application thereof, and belongs to the technical field of antiviral preparations. A scorpion tail electricity-saving stimulation method is adopted to obtain crude scorpion venom, and centrifugation and freeze drying are performed to obtain scorpion venom freeze-dried powder; dissolving the scorpion toxin freeze-dried powder, filtering, removing sediment, carrying out ultrafiltration centrifugation, and separating to obtain the anti-EV71 virus activity scorpion toxin polypeptide. According to the present invention, the scorpion martensii toxin polypeptides S-I (lt; 3kDa) and S-II (3-10 kDa) obtained from the scorpion martensii have good EV71 virus resistance; under the same concentration, the relative molecular mass is lt; compared with the prior art, the scorpion margaritae toxin polypeptide with the molecular weight of 3kDa has better anti-EV71 virus activity, has no toxic effect on cells, has the potential of being used as an anti-EV71 virus drug, and provides a theoretical basis for biomedical research and clinical application of the scorpion margaritae toxin polypeptide.

The invention discloses composite hydrogel as well as a preparation method and application thereof. The curcumin-EGCG composite hydrogel is prepared by forming a complex through hydrogen-bond interaction between gelatin and epigallocatechin gallate and carrying out chemical crosslinking through Schiff base reaction of curcumin and gelatin. The composite hydrogel disclosed by the invention has a self-healing characteristic or viscosity and a shear thinning characteristic, and is beneficial to local application. The composite hydrogel is loose and porous in structure, the drug loading rate can be increased, the drug can be slowly and controllably released, the treatment effect of the composite hydrogel in androgenetic alopecia is promoted, meanwhile, the solubility of a drug monomer can be remarkably improved, and the problem that the drug solubility is low is solved. The composite hydrogel provided by the invention provides an effective transdermal drug delivery preparation for treatment of skin diseases, and has good clinical application prospects and development values.

The invention belongs to the field of daily chemicals, and discloses a strain of bifidobacterium adolescentis, the classification name of the bifidobacterium adolescentis BF23BA001 is bifidobacterium adolescentis, the preservation number is CGMCC NO.26999, and the strain of bifidobacterium adolescentis BF23BA001 is named as bifidobacterium adolescentis BF23BA001. The preservation date is March 31, 2023, and the preservation unit is China General Microbiological Culture Collection Center (CGMCC); the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Compared with other commercially available similar bacteria, the strain has unique advantages in the production of small molecule peptides and gamma-aminobutyric acid, has a remarkable anti-wrinkle advantage according to the functional characteristics of the small molecule peptides and gamma-aminobutyric acid, and has a remarkable anti-wrinkle effect through subsequent functional verification. The method is of great significance to further improvement of whitening, brightening, repairing and anti-aging effects of yeast fermentation products. In addition, the invention further discloses a composition for inhibiting generation and accumulation of lipofuscin, application based on the composition and cosmetics.

The invention belongs to the field of drug preparation, and particularly relates to a preparation method and application of a drug delivery system based on hollow mesoporous polydopamine nanoparticles. The drug delivery system comprises hollow mesoporous polydopamine nanoparticles, a drug loaded inside and / or outside the hollow mesoporous polydopamine nanoparticles, and pectin wrapping the hollow mesoporous polydopamine nanoparticles, the medicine is a medicine with a nerve protection effect and an acetylcholin esterase inhibitor. The drug delivery system can reduce proinflammatory cytokines, regulate functions of target immune cells, synergistically reduce necrosis of neurons in intestinal tracts and induce neural crest stem cells in the intestinal tracts to be differentiated into neurons.

The invention belongs to the technical field of anti-fingerprint coating materials, and provides a tung oil derivative composite fluoride-free anti-fingerprint transparent coating material as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing a polydimethylsiloxane modified polyurethane acrylate prepolymer, a tung oil-based photoactive monomer, a photoinitiator and an organic solvent, coating a pretreated base material with the mixed coating, and carrying out ultraviolet curing to obtain the tung oil derivative composite fluoride-free fingerprint-resistant transparent coating material. The coating material disclosed by the invention has an excellent hydrophobic effect and excellent fingerprint resistance; the tung oil-based photoactive monomer can increase the content of bio-based substances in the coating and improve the green environmental protection property of the coating. The transparent coating provided by the invention has a water contact angle close to 110 degrees, light transmittance of 85% or more, and fingerprint resistance that fingerprints can be removed only by wiping the transparent coating with tissues for 4 times or less.

The invention discloses a preparation method and application of collagen microspheres, and belongs to the technical field of genetic engineering. Two histidines are respectively inserted into the N end and the C end of the collagen B-domain amino acid sequence, the high polymerization self-assembly of the collagen is promoted by utilizing the metal chelating effect to form a three-dimensional network structure, and a histidine is further inserted into the middle of the collagen, so that the three-dimensional morphology transformation of the collagen assembly is realized, and a loose and porous microsphere structure is formed. Experiments prove that by controlling the number and position of His residues in the collagen B-domain amino acid sequence expressed by microbial cells, the three-dimensional morphology of collagen can be controlled, and the collagen microspheres are formed; the prepared collagen microspheres can significantly promote cell adhesion, extension and migration.

The invention discloses a preparation method of a nanofiber membrane capable of adsorbing various perfluorinated and polyfluoroalkyl substances, which comprises the following steps of: obtaining a nanofiber membrane spun by mixing quaternized chitosan and polyvinyl alcohol by adopting an electrostatic spinning and glutaraldehyde crosslinking method, and directly immersing the nanofiber membrane into environmental water for use; short-chain perfluorocarboxylic acid, long-chain perfluorocarboxylic acid, short-chain perfluorosulfonic acid, long-chain perfluorosulfonic acid and perfluoroalkyl ether carboxylic acid in tap water, surface water, underground water, domestic sewage, industrial sewage, agricultural sewage or medical wastewater can be efficiently and simultaneously adsorbed and removed; various types of perfluoro and polyfluoroalkyl substances such as perfluoro nonoxybenzene sulfonates and chlorinated polyfluoroether sulfonates can be selected from various types of perfluoro and polyfluoroalkyl substances. And after adsorption removal is completed, taking out the wastewater from the environmental water to complete solid-liquid separation. Compared with an existing adsorbent, the adsorbent has the advantages that more types of perfluorinated and polyfluoroalkyl substances can be adsorbed and removed at the same time, the adsorption and removal efficiency on short-chain perfluorinated substances is higher, and the applicable environment water matrix is wider.

The invention relates to the technical field of building materials, and provides a high-durability modified cement-based composite material and a preparation method thereof.The high-durability modified cement-based composite material comprises a carbon dot polymer, mixing water and Portland cement, and the specific preparation steps are as follows: adding citric acid and urea into water to be dissolved, and then putting into a high-pressure reaction kettle to be heated to obtain an initial product; adding the initial product into water, and carrying out centrifugation, filtration, rotary evaporation and drying to obtain the carbon dots; the preparation method comprises the following steps: dissolving carbon dots in water, preparing an initial carbon dot polymer product by adopting a hydrothermal method or a microwave method, adding the initial carbon dot polymer product into water, centrifuging, filtering, rotationally evaporating and drying to obtain a carbon dot polymer; and mixing the carbon dot polymer with mixing water and Portland cement to obtain the high-durability modified cement-based composite material. The modified cement-based composite material prepared by the invention is simple to prepare, low in cost, green and low-carbon; the rust can be efficiently inhibited, and concrete reinforcements are effectively protected from being corroded; the method can be widely applied to reinforced concrete and has wide application prospects.

The invention discloses a compound plant stress-resistant agent as well as a preparation method and application thereof. The compound plant stress-resistant agent comprises a compound of glycine betaine and polyglutamic acid, wherein the mass ratio of the glycine betaine to the polyglutamic acid is 1: (0.1-2). The preparation method of the composite plant stress-resistant agent comprises the following steps: mixing polyglutamic acid or a solution thereof with glycine betaine in ethanol to obtain a mixed solution, adjusting the pH value of the mixed solution to 3.0-6.0, carrying out an ultrasonic reaction to obtain a reaction solution, adding absolute ethyl alcohol into the reaction solution, stirring, filtering, and drying the precipitate to obtain the composite plant stress-resistant agent. The compound plant stress-resistant agent can be applied to crops in a foliage spraying or root irrigation mode, the growth conditions of grain crops such as corn, peanuts, celery and grapes and / or economic crops under adversity stress such as drought, low temperature and high salt are remarkably relieved, and the crop yield is increased.

The present invention provides a high brain targeting, safe and noninvasive nasal-brain delivery preparation, which is characterized in that the nasal-brain delivery preparation comprises nimodipine, a good solvent, a diluent and an absorption enhancer, the good solvent is diethylene glycol monoethyl ether, and the absorption enhancer is phospholipid. The nasal-brain delivery preparation is prepared from a good solvent, a diluent, an absorption enhancer and the like according to a specific proportion. Nimodipine serving as an active ingredient is enriched in cerebrospinal fluid and brain tissues through a specific upper nasal meatus administration technology, so that the treatment effect of nimodipine is achieved; and meanwhile, the blood entering proportion is reduced, so that the cardiovascular toxicity and hepatotoxicity are reduced. In addition, the preparation formula has no irritation to local parts of the nasal cavity, and the compliance of a patient is improved.

The invention relates to the technical field of drugs for treating thyroid cancer, in particular to application of honeysuckle polysaccharide in preparation of drugs for treating thyroid cancer. According to the technical scheme, on the basis of separating and purifying the honeysuckle polysaccharide with relatively good uniformity, the honeysuckle polysaccharide is found to have no toxic effect on normal thyroid follicular cells, but selectively inhibits proliferation of recurrent metastatic papillary thyroid carcinoma cells, and has an inhibiting effect on transplanted tumors of nude mice inoculated with a recurrent metastatic papillary thyroid carcinoma cell line. According to the scheme, the honeysuckle flower polysaccharide is good in homogeneity, the molecular weight is 23 + / -4 kDa, the honeysuckle flower polysaccharide is mainly composed of five monosaccharides, and alpha-(1-> 4)-D-GalpA is mainly connected to form a main chain. The polysaccharide has no toxic effect on normal thyroid cells while effectively inhibiting the cell viability of IHH-4. According to the technical scheme, the technical problem that drugs for treating recurrent metastatic papillary thyroid carcinoma with small side effects and high specificity are lacked in the prior art can be solved, and the drug has an ideal application prospect.

The invention discloses a puncture-resistant biodegradable film bag and a preparation method thereof. The puncture-resistant biodegradable film bag is prepared from the following raw materials in parts by weight: 50-80 parts of biodegradable polyester, 1-20 parts of a modifier and 10-35 parts of an inorganic filler, wherein the total part number of the three raw materials is 100; the composition further comprises 0.1-5 parts of a cross-linking agent, 0.1-5 parts of an antioxidant and 0.1-1 part of a dispersing agent; the weight-average molecular weight of the puncture-resistant biodegradable material is 100,000-300,000; and the cross-linking agent is at least one of triallyl isocyanurate, trimethylallyl isocyanate, trimethylolpropane triacrylate, trimethylolpropane trimethacrylate and triglycidyl tripolyisocyanate. The modifier, the cross-linking agent and the biodegradable polyester have a synergistic effect, and through an electron beam irradiation process, not only can the puncture strength of the film bag be greatly improved, but also the MD tensile strength and the TD tensile strength are also greatly improved.

The invention relates to a preparation method of a cannabidiol nano-lipid carrier, in particular to a preparation method of a cannabidiol nano-lipid carrier with a transdermal absorption function. The invention aims to solve the problem that the existing cannabidiol lipid carrier cannot have high water solubility and high transdermal absorption function at the same time, and is poor in stability and easy to irritate skin. The method comprises the following steps: 1, weighing raw materials; 2, preparing an oil phase; 3, preparing a water phase; 4, mixing; 5, performing ultrasonic homogenization; 6, cooling; and 7, removing impurities. The preparation method is used for preparing the cannabidiol nano-lipid carrier with the transdermal absorption function.

The invention provides a Dendrobium officinale Kimura amp (MIG) leaf extract, a preparation method of the Dendrobium officinale Kimura amp (MIG) leaf extract, and application of the Dendrobium officinale Kimura amp (MIG) leaf extract in the fields of cosmetics and medicines. Belongs to the technical field of cosmetics and medicines. The dendrobium officinale leaf extract WYH169, the dendrobium officinale leaf extract WYH2202, the dendrobium officinale leaf extract WYH205 and the dendrobium officinale leaf extract WYH328 have the effect of promoting collagen secretion in human dermis fibroblasts (human dermis fibroblasts, HDFa), and can be used for preparing anti-aging drugs or skin care products, so that the dendrobium officinale leaf extract WYH169, the dendrobium officinale leaf extract WYH2202, the dendrobium officinale leaf extract WYH205 and the dendrobium officinale leaf extract WYH328 can be used for preparing anti-aging drugs or skin care products.

The invention discloses a cationic polyester, comprising a copolymer of monomer C, monomer S and monomer M, wherein the copolymer terminal group is modified with a group E; the monomer C comprises cholesterol; the monomer S comprises an organic acid having at least two carboxyl groups at the terminal group; the monomer M comprises a compound containing two hydroxyls and at least one secondary amine or tertiary amine; and the compound modified with the group E comprises at least one primary amine, secondary amine or tertiary amine group. The in vitro cell-level mRNA delivery efficiency of the cationic polyester of the invention is significantly better than that of the existing commonly used commercial gene transfection reagents, and the cytotoxicity is lower; the cationic polyester is soluble in ethanol, and is easy to be industrialized and clinically applied in preparations, and therefore has great practical application prospects.

The invention provides an aquatic bait biological feed as well as a preparation method and application thereof. Wherein the aquatic bait biological feed comprises a yeast raw material, amino acid raw powder and feed-grade fish oil; wherein the yeast raw material is selected from at least one of yeast metabolite, yeast powder, yeast hydrolysate or yeast cell wall dry powder. The components in the aquatic bait biological feed are appropriate in particle size, can be directly ingested and absorbed by zooplanktons such as rotifers and cladocerans, are easily digested and absorbed by the zooplanktons, and are beneficial to growth and reproduction of bait organisms. In addition, the feed raw materials do not have the problems of pathogenic bacteria and drug residues, and do not have toxicity to bait organisms.

The invention discloses a power cable ethylene propylene rubber insulating rubber material, and relates to a power cable insulating rubber material. The problem that an existing ethylene propylene rubber insulating material is low in modulus and tensile strength is solved. The power cable ethylene propylene rubber insulation sizing material comprises the following components in parts by weight: ethylene propylene rubber 2470, ethylene-vinyl acetate copolymer, ethylene propylene rubber 4044, dicumyl peroxide, high-density paraffin oil, low-density paraffin oil, microcrystalline wax, white carbon black, superfine talcum powder, modified calcined clay, vinyl trimethoxy silane, 2-thiol benzimidazole, 2, 2, 4-trimethyl-1, 3-pentanediol monoisobutyrate, a coupling agent, a coupling agent and an antioxidant. The catalyst is prepared from the following raw materials: a 1, 2, 4-trimethyl-1, 2-dihydroquinoline polymer, dicumyl peroxide and zinc oxide. The power cable ethylene propylene rubber insulation rubber material is simple in formula, improves the flame retardant property of the insulation material, reduces the cost of the insulation material, has the characteristics of excellent electrical performance, good processing technology and the like, also has good mechanical and physical properties, and promotes the popularization and application of ethylene propylene rubber insulation middle and high voltage power cables.

The invention relates to the technical field of carbon quantum dots, in particular to a high-molecular fluorescent carbon dot with PBAT as a carbon source, a preparation method of the high-molecular fluorescent carbon dot and application of the high-molecular fluorescent carbon dot to detection of iron ions. The preparation method comprises the following steps: oxidizing a copolymer (PBAT) of butylene adipate and butylene terephthalate with air, then carrying out hydrothermal treatment in an H2O2 aqueous solution to prepare CDs, putting PBAT into an open ceramic crucible, and carrying out air oxidation to form a dark brown oxidation product. Then, a product is transferred into a reaction kettle containing an H2O2 solution; after hydro-thermal treatment, CDs are brown and dispersed. And carrying out centrifugation, dialysis and freeze drying on the CDs dispersion liquid to finally obtain CDs powder. The carbon quantum dot prepared by the invention is synthesized by a hydrothermal method in one step, has stable fluorescence characteristics and is non-toxic. The efficient fluorescent carbon quantum dot can be used as a fluorescent probe for quantitative measurement of iron ions.

The invention discloses a mixed alkali liquor carbon dioxide trapping agent and a trapping method thereof. The trapping agent comprises the following components in percentage by mass: 22-38% of main absorbent sodium carbonate, 1-10% of auxiliary absorbent A organic amine, 2-7% of auxiliary absorbent B sodium phosphate and / or disodium hydrogen phosphate and the balance of water. When the carbon dioxide is captured, the gas containing the carbon dioxide is in full contact with the mixed alkali liquid carbon dioxide capturing agent, the capturing agent absorbs the carbon dioxide to generate sodium bicarbonate precipitate, and the separated sodium bicarbonate solid is heated and desorbed at the temperature of 100-180 DEG C to obtain sodium carbonate, the carbon dioxide and water; and finally, returning the analyzed sodium carbonate to the mixed alkali liquor for recycling, and dehydrating, drying and purifying the mixed gas of carbon dioxide and water to obtain carbon dioxide. The trapping agent has the advantages of low cost, high carbon dioxide absorption capacity, high absorption rate, low regeneration energy consumption, safety and no toxicity; the sodium carbonate saturated solution is activated by using organic amine, so that the carbon dioxide absorption efficiency is improved; the separated sodium bicarbonate is heated and decomposed to obtain carbon dioxide, so that the working energy consumption is greatly reduced.

The invention discloses a photosensitizer for endogenous hydrogen sulfide activation and visible light excitation as well as a preparation method and application thereof, and relates to the technical field of medical photosensitizers. According to the photosensitizer provided by the invention, BA and NIS react to obtain a BODIPY compound I2BA substituted by double atoms I, so that the intersystem crossing capability is enhanced, and the yield of active oxygen is increased; a target photosensitizer is obtained by introducing a tetraazobenzene ring Cyclone into the para-position of a benzene ring and combining copper ions Cu < 2 + >. The prepared photosensitizer has good light stability and water solubility, can be activated by endogenous hydrogen sulfide in cancer cells, can generate singlet oxygen (1O2) under the irradiation of green light (520 nm), shows excellent photodynamic activity to tumor cells and low dark toxicity to normal cells, has a high photodynamic activity index (PI value), and can be used as a photosensitizer for cancer cells. The compound is successfully used for photodynamic inhibition and elimination of tumors on HCT116 tumor-bearing mice.

The present invention relates to the field of multifunctional rubber additives, and specifically discloses a multifunctional rubber additive polyazole selenium and its preparation method and application. The method comprises: dissolving selenium dioxide and polyazole ligands in anhydrous ethanol respectively, then dropping the ethanol solution of selenium dioxide into the ethanol solution of polyazole under heating and stirring, and continuously reacting to obtain a solid precipitate; and filtering, washing, and drying the solid precipitate to obtain the multifunctional rubber additive polyazole selenium. The polyazole selenium rubber multifunctional additive prepared by the present invention can significantly improve the thermal oxidation aging resistance of the material, can stably exert the anti-aging effect for a long time, and has the advantage of reducing the amount of other additives used. At the same time, the preparation method of the present invention is environmentally friendly and pollution-free, low in cost, and high in yield.

The invention discloses a novel collagen functional variant amino acid and functional application thereof, and relates to the technical field of collagen variants, the amino acid of the collagen functional variant is a COL fragment which is extracted from full-length human COL1 and has the length of 30 amino acids, and a collagen variant C109 synthesized by polypeptide has the structural formula shown in the description. It is verified that the variant has a self-assembly structural property and a good biological activity function, and the amino acid sequence of the collagen functional variant is GLPGPSGEPGKQGPSGASGERGPPGPMGPP (SEQ ID No. 1); the polypeptide formed by the collagen functional variant provided by the invention has the same self-assembly capability as full-length collagen, has better biological activity, is non-toxic, can promote osteoblast differentiation, and supports tissue regeneration at the same time.

The invention discloses application of carboline compounds in preparation of products for reducing accumulation of advanced glycosylation end products, and belongs to the technical field of preparation of anti-saccharification products. The carboline compound not only can reduce the generation of AGEs in a BSA-MGO pure in-vitro AGEs generation model, but also can effectively reduce the level of AGEs in a glucose-induced intracellular AGEs generation model, has a remarkable AGEs inhibition effect, has no toxic effect on cells, has good safety, and can meet the application requirements of anti-saccharification products.

The invention belongs to the technical field of agricultural cultivation, and particularly relates to a method for inducing industrial cannabis sativa to generate female seeds through an inducer. The method comprises the following steps: (1) preparing an inducer mixed solution; the concentration of the nano-silver particles in the inducer mixed solution is 150 mg / L to 200 mg / L, the concentration of the gibberellin is 80 mg / L to 100 mg / L, and the concentration of the surfactant is 500 mg / L to 700 mg / L; (2) selecting industrial cannabis sativa which is about 10-15 days in the flowering period and is confirmed to be female plants, spraying the whole female plants of the industrial cannabis sativa in a mist form by using the inducer mixed solution in the near evening, avoiding stems, and preferably, spraying once every 2-3 days, spraying twice in total, and meanwhile, changing the illumination time, so that the female plants of the industrial cannabis sativa are obtained. The obtained industrial hemp seeds are all female. The method disclosed by the invention is high in all-female induction rate and good in stability.

The invention provides a pedicel and inflorescence extract of Gastrodia elata Blume, a preparation method of the pedicel and inflorescence extract of Gastrodia elata Blume, and application of the pedicel and inflorescence extract of Gastrodia elata Blume in the fields of cosmetics and medicines. Belongs to the technical field of cosmetics and medicines. The gastrodia elata pedicel and inflorescence extracts ZTM-2, ZTM-4, ZTM-5, ZTM-6, ZTM-7 and ZTM-8 have the effect of promoting collagen secretion, and the ZTM-5 and the ZTM-6 have the activity of promoting collagen secretion in human dermis fibroblasts (human dermis fibroblasts, HDFa) at the extremely low concentration (1 mu g / mL), and have the activity of promoting collagen secretion in human dermis fibroblasts (human dermis fibroblasts, human dermis fibroblasts) at the extremely low concentration (1 mu g / mL) at the extremely low concentration (1 mu g / mL) at the extremely low concentration (1 mu g / mL) at the extremely low concentration (1 mu g / mL) at the extremely low concentration (1 mu g / mL). Under the concentration, the compound has no cytotoxicity and can be used for preparing anti-aging drugs or skin care products.

The invention relates to an orange red copper-based halide luminescent material and a preparation method thereof, and belongs to the technical field of luminescent materials. The molecular formula of the luminescent material is (TPP) 2Cu4Br6. 2DMSO, and the luminescent material belongs to a Pbcn space group in an orthorhombic system, presents broadband orange-red light emission under ultraviolet excitation, has the characteristics of no toxicity, broadband emission, high fluorescence quantum yield, low cost and the like, and shows a huge application prospect in the field of solid-state illumination. According to the method, a single crystal of the luminescent material is obtained by adopting a low-temperature cooling crystallization method, and a micro crystal of the luminescent material is obtained by adopting an anti-solvent method; the preparation method has the characteristics of simplicity in operation, short reaction time, suitability for batch production and the like, and is beneficial to popularization and application of the luminescent material.

The invention discloses a super-hydrophobic metal organic framework composite material and a preparation method and application thereof, and the preparation method comprises the following steps: mixing an amino-modified ZIF material with polylactic acid with the number-average molecular weight of 1.0 * 105 g / mol to 2.5 * 105 g / mol, and carrying out amidation reaction to obtain the super-hydrophobic metal organic framework composite material. In the invention, the amino-modified ZIF material is modified by polylactic acid, so that the super-hydrophobic metal-organic framework composite material which is low in cost, good in hydrophobicity, good in stability, green and environment-friendly can be prepared, and the super-hydrophobic metal-organic framework composite material can be widely applied to wastewater treatment, air purification and sensor preparation; the method has potential application in the aspects of solving the greenhouse effect, industrial gas adsorption and separation, oil-water separation and the like, and has a good application prospect. The preparation method also has the advantages of simple process, convenience in operation, mild preparation conditions and the like, is suitable for large-scale preparation, and is convenient for industrial application.

The invention discloses a magnesium boride nanosheet / inulin composite hydrogel and application thereof in treating inflammatory bowel diseases and complicated mental diseases, and the composite hydrogel is synthesized by doping magnesium boride nanosheets into inulin and carrying out hot melting and cooling to form gel. The composite hydrogel has good oxidation resistance and biocompatibility, reducing hydrogen can be generated through a hydrolysis reaction to be used for reducing active oxygen and inflammatory factors of the enteritis part, intestinal flora can be adjusted, meanwhile, the composite hydrogel can be prevented from being affected by gastric acid and reach the intestinal part through the addition of inulin, and the enteritis treatment effect is improved. Therefore, the inflammatory bowel disease and related anxiety and depression symptoms thereof are effectively treated.

According to the silk fibroin hydrogel microneedle manufacturing method and the hydrogel microneedle, a silk fibroin solution and a riboflavin aqueous solution are evenly mixed to obtain a mixed solution, then a hydrogen peroxide solution is added into the mixed solution to be mixed to obtain a precursor solution, then the precursor solution is poured into a light-transmitting microneedle mold, and the silk fibroin hydrogel microneedle is obtained. Placing a microneedle mold containing the precursor solution into a vacuum pump, performing vacuum pumping treatment, taking out the microneedle mold, placing the microneedle mold under ultraviolet irradiation for illumination to form a gel, drying the gel at room temperature, taking out the gel from the microneedle mold, forming a plurality of convex cones on the outer surface of the gel, and performing vacuum pumping treatment on the cones to obtain the composite microneedle. The silk fibroin hydrogel microneedle is obtained. According to the design, the operation requirement is low, the slow release effect is good, the biocompatibility is high, and efficient carrying of treatment medicine is facilitated.

The present application relates to the technical field of biological medicine, and discloses inhalable aptamer modified molybdenum disulfide nanoflower compound and application thereof.The compound comprises a molybdenum disulfide nanoflower core, a dendritic polylysine modification layer attached to the surface of the molybdenum disulfide nanoflower, doxorubicin loaded on the molybdenum disulfide nanoflower and / or the dendritic polylysine modification layer, and AS1411 aptamer combined on the outer surface of the dendritic polylysine modification layer by electrostatic assembly.The compound can be delivered to a lung lesion by atomization inhalation or aerosol inhalation, and undergoes responsive structural change under the action of hydrogen peroxide in a tumor microenvironment, generates active oxygen, and promotes the release of doxorubicin, so as to realize the synergistic antitumor effect of chemotherapy and chemical dynamic therapy.The compound has good colloidal stability, drug loading capacity, active targeting ability and lung local delivery potential, and can be used for preparing inhalation preparations or local administration preparations for treating lung cancer.