15 results about "Microbial polysaccharides" patented technology

The present invention relates to a method for preparing microbial exopolysaccharides and their use in tissue culture medium, belonging to the technical field of microbial polysaccharide preparation. The method for preparing microbial exopolysaccharides utilizes batch fermentation of different microorganisms, adds a beet extract and a supplement to the culture medium to promote microbial exopolysaccharide production, and further purifies, enzymatically hydrolyzes, and separates the exopolysaccharides. The resulting target microbial exopolysaccharides have a good effect on promoting the development and rooting of tissue culture seedlings and can be used to prepare tissue culture medium.

This invention proposes a combined inhibitor for separating bastnaesite and monazite, along with its method and application. The raw materials for the combined inhibitor include small organic molecules and microbial polysaccharides; the small organic molecules include one or more of oxalic acid, malic acid, tartaric acid, lactic acid, succinic acid, and citric acid; the microbial polysaccharides include one or more of currant gum, guar gum, xanthan gum, and gellan gum. This invention achieves highly selective inhibition of monazite on the mineral surface through the synergistic adsorption and action of the inhibitor, with small organic molecules and microbial polysaccharides as the main components, thereby efficiently separating bastnaesite under mild flotation conditions. It offers advantages such as good selectivity, strong action, low reagent cost, and environmental friendliness.

To provide a composition and a method capable of storing one or more microorganisms in a liquid state for a long period of time at high density without functional deterioration of the microorganisms and while suppressing contamination by other microorganisms.SOLUTION: Provided is a plastic fluid composition comprising: (1) a microorganism; and (2) a polysaccharide or nanofibers made of the polysaccharide, where when the microorganism is a bacterium, the microorganism is contained at a microbial density of at least 1×107 CFU / mL and dispersed in the composition, and when the microorganism is a fungus, the microorganism is contained at a microbial density of at least 1×103 CFU / mL and dispersed in the composition.SELECTED DRAWING: None

This invention provides the application of *Ackermania guangxiensis* extracellular polysaccharide in the preparation of anti-aging products, belonging to the technical field of microbial polysaccharide pharmaceutical applications. This invention extracts extracellular polysaccharides from *Ackermania guangxiensis* using the TCA precipitation method. Animal experiments show that *Ackermania guangxiensis* extracellular polysaccharide can effectively reduce MDA content in aging model mice while increasing SOD activity; it improves the thinning of the dermal layer, reduction and loosening of collagen fibers, and other signs of aging in model mice, and increases the hydroxyproline content in the skin; furthermore, it can activate the expression of BDNF and TrKB genes in the mouse hippocampus and alleviate problems such as slow movement, cognitive impairment, and memory decline in model mice. Therefore, the *Ackermania guangxiensis* extracellular polysaccharide provided by this invention has good anti-aging and antioxidant activity and can be used to prepare various anti-aging products.

The application belongs to the technical field of polysaccharide preparation and relates to a purification method and application of microbial polysaccharide hyaluronic acid. ‑ The viscosity of the hyaluronic acid fermentation liquor is reduced by more than 99% after the alkali-adjusted and viscosity-reduced hyaluronic acid fermentation liquor is subjected to mechanical shearing treatment, so that the viscosity is further reduced to 40-75 mPa s; the hyaluronic acid fermentation liquor after the high-shear treatment is filtered to remove bacteria and impurities; acid is added to the filtered solution for neutralization, and then alcohol precipitation and drying are performed, and the hyaluronic acid is obtained. The application can quickly reduce the viscosity of the hyaluronic acid fermentation liquor to reach the viscosity range for efficient bacteria removal and impurity removal in subsequent operations, thereby breaking through the industrial bottleneck of bacteria removal and purification in the industrialized production process of microbial polysaccharide hyaluronic acid.

The utility model relates to the technical field of welan gum fermentation, and discloses a high-viscosity microbial polysaccharide welan gum fermentation device which comprises a fermentation tank body and supporting legs fixedly arranged at four corners of the lower end of the fermentation tank body. After the device is used, the stirring head needs to be detached and cleaned, when the device is used for preparing welan gum, various required raw materials can be added into the fermentation tank body through the feeding pipe, and then the motor is started to drive the stirring structure through the output end of the motor to mix and stir the raw materials in the fermentation tank body; after long-time fermentation, the movable cover can be directly lifted upwards, at the moment, the movable cover, the motor and the stirring structure extending into the fermentation tank body can be completely taken out, after stirring fermentation is completed, the stirring structure can be completely taken out through the movable cover to be cleaned, fermented materials with high viscosity can be conveniently cleaned, and the fermentation efficiency is improved. The subsequent fermentation operation is convenient.

This invention relates to a polyvinylidene fluoride (PVDF) piezoelectric material, its preparation method, and a piezoelectric power generation device. The piezoelectric material, by weight, comprises 1-1.5 parts of PVDF and 0-0.3 parts of pullulan, wherein the endpoint 0 is unavailable. In this invention, the long chains of pullulan are extensively entangled with PVDF and form a physical cross-linking network through hydrogen bonds, greatly restricting the mobility of the PVDF molecular chains and "locking" the formed β-phase conformation, preventing it from relaxing back to the α-phase. This stabilizing effect ensures that a high content of the β-phase is retained in the final material, thereby improving the piezoelectric properties of the material. The PVDF piezoelectric material of this invention has a simple composition, and the pullulan used is a special microbial polysaccharide, giving it good biodegradability and broad application prospects.

The invention relates to microbial polysaccharide for improving the production performance of laying hens and a preparation method thereof, and belongs to the technical field of microbial polysaccharide. The microbial polysaccharide is prepared from fermentation products of saccharomyces cerevisiae, paenibacillus polymyxa, bacillus natto and xanthomonas campestris, crude polysaccharide is extracted from fermentation liquor, then separation is conducted through high-speed counter-current chromatography, and then purification is conducted to obtain target polysaccharide. The obtained microbial polysaccharide can obviously improve the egg laying performance of laying hens.

The invention discloses a microbial polysaccharide extractor, and relates to the technical field of polysaccharide extraction, four corners of the bottom of a reaction tank are fixedly provided with No.1 support legs, and the upper end of the reaction tank is connected with a feed hopper in a penetrating manner; the stirring mechanism is arranged in the reaction tank; the mechanism is arranged on the reaction tank; the filtering mechanism is arranged on the right side of the reaction tank; the moving mechanism is arranged in the filtering mechanism; the stock solution can be rapidly and uniformly heated, so that the stock solution can fully react; and residues can be filtered, and the residues can be conveniently cleaned or recycled.

This invention relates to the field of cementing technology, disclosing a water-indispersible composition, a cement slurry containing the same, its preparation method, and its applications. The water-indispersible composition comprises: 0.5-5 parts by weight of a microbial polysaccharide polymer, 5-50 parts by weight of an organic polymer, and 4.5-45 parts by weight of inorganic particles. The water-indispersible composition of this invention exhibits good water resistance within a temperature range from room temperature to 180°C, and can be used to prepare cement slurries. It ensures that the particles in the cement slurry are not dispersed by formation water, while having no adverse effects on the conventional properties of the cement slurry, such as rheology, water loss, stability, and cement stone strength. This invention also provides a cement slurry containing the water-indispersible composition. This cement slurry exhibits water resistance, ensuring effective filling of the annulus cement slurry and the integrity of the cement sheath structure. It is suitable for sealing high water-cut layers and plugging lost circulation zones, achieving effective sealing of high water-cut layers and effective plugging of lost circulation zones at different well depths.

Antibodies and antigen-binding fragments thereof are provided that bind beta-1,6-poly-N-acetyl glucosamine (PNAG) that has been deacetylated in whole or part (dPNAG). Compositions and kits comprising these antibodies and antigen-binding fragment thereof are also provided. Such compositions can include, for example, diagnostic and therapeutic compositions. Methods of using these antibodies and antigen-binding fragments thereof are further provided. Such methods can include, for example, methods for preventing, diagnosing, and treating bacterial infections and associated biofilms characterized by PNAG and dPNAG.

The invention belongs to the technical field of compound fertilizers, and relates to a universal microbial polysaccharide compound fertilizer and a preparation method thereof. The universal microbial polysaccharide compound fertilizer is prepared from the following raw materials in parts by mass: 180 to 200 parts of potassium chloride, 200 to 250 parts of monoammonium phosphate, 70 to 95 parts of ammonium chloride, 450 to 500 parts of urea, 20 to 38 parts of calcium powder, 6 to 8 parts of microbial polysaccharide, 1 to 4 parts of anti-caking paste and 3 to 7 parts of anti-caking powder. The universal microbial polysaccharide compound fertilizer disclosed by the invention can be used for remolding a soil aggregate structure, reducing soil hardening and nutrient loss, achieving the effects of improving soil and retaining fertilizer, ensuring many white roots and developed capillary roots, and improving the fertilizer and water absorption capacity. The four-layer structure of the compound fertilizer enables all the components to play a role, and all the elements are absorbed by crops to the maximum extent.

The application discloses a method for improving gene transformation efficiency of a microorganism polysaccharide-producing strain, and the method comprises the following steps: treating the microorganism polysaccharide-producing strain by using a polysaccharide-degrading enzyme, removing the wrapping of the polysaccharide on the strain cell, obtaining the cell, and preparing a competent cell; then, an exogenous plasmid is transformed into the treated competent cell by using an electric shock; wherein the polysaccharide-degrading enzyme can degrade the extracellular polysaccharide produced in the culture process of the microorganism polysaccharide-producing strain. The application effectively improves the success rate and the transformation efficiency of plasmid transformation.

A recombinant meat binder composed of microbial polysaccharides and TG enzyme, its application, and a method for preparing recombinant meat are disclosed. The recombinant meat binder is composed of TG enzyme (transglutaminase), xanthan gum, and chitosan, wherein the weight ratio of TG enzyme:xanthan gum:chitosan is (12-16):(1.5-3):(3-5). This invention also discloses the application of this recombinant meat binder composed of microbial polysaccharides and TG enzyme in recombinant meat processing, and a method for preparing recombinant meat using this recombinant meat binder. In this recombinant meat binder composed of microbial polysaccharides and TG enzyme, xanthan gum and chitosan oligosaccharides enhance water retention through physical adsorption and hydrogen bonding, while TG enzyme catalyzes the cross-linking of protein molecules to form a gel network. These synergistic effects significantly improve the texture, water retention, gel strength, and nutritional value of processed mutton mince, achieving a gel strength of 6.66 N.

The invention provides a degradable farmland wind prevention and sand fixation agent and a preparation method thereof, and relates to the field of environmental governance materials. Comprising the following raw materials: 80-90% of deionized water, 2-8% of plant polysaccharide, 1-2% of microbial polysaccharide, 5-15% of organic high-molecular polymer and 0.1-0.5% of inorganic salt. The sand stabilizing agent has the advantages of excellent sand stabilizing effect, high permeability, no pollution to the environment, low cost and convenience in application and scattering.