166 results about "Microbial polysaccharides" patented technology

The present invention provides a controlled release device for sustained or pulsatile delivery of pharmaceutically active substances for a predetermined period of time. This invention further provides such device in which sustained or pulsatile delivery is obtained by the unique blend and intimate mixture of pharmaceutically active substances with a microbial polysaccharide and uncrosslinked linear polymer and optionally a crosslinked polymer and / or lipophillic polymer and / or saturated polyglycolyzed glyceride. The invention also provides a process for the manufacture of such devices and pharmaceutical compositions containing the same.

The invention provides an ecological environment-friendly dust inhibitor which comprises the following components by weight percent: 0.5-0.8% of modified starch, 0.05-0.10% of microbial polysaccharide, 0.1-0.3% of cellulose derivative, 0.05-0.10% of netty accelerant, 0.05-0.15% of flow dispersant, 0.01-0.10% of film forming agent and 98.0-99.0% of water. The dust inhibitor can enable coal or mineral powder to form a layer of curing film on the surface, is high in viscosity and low in wind erosion rate, can avoid the flowing dust pollution and the flowing dust loss of the coal or the mineral powder to the way side in the railway transportation process, and has a huge economic benefit and a social benefit of environmental protection for railway transportation and coal or mineral powder customers; and the components and a solution of the dust inhibitor do not have any toxic side effect and corrosive effect, the dust inhibitor does not cause any secondary pollution as the dust inhibitor can be quickly and biochemically degraded in the nature after the dust inhibition action period, and the soil can be properly improved.

The invention discloses a making method of sphingol single-cell bacterium and microbe polysaccharide, which comprises the following steps: inoculating sphingol single-cell bacterium CGMCCNo. 1650 in the culture liquid with carbon source; adding necessary nitrogen and nourishing material; adjusting pH value of culture liquid; aerating; stirring; fermenting; adjusting isoelectric point through neutral salt for fermented liquid; sedimenting; extracting; dehydrating; obtaining the product.

The invention relates to a method for preparing a microbial polysaccharide modified copolymer gel plugging agent. The method comprises the following steps: adding an amide monomer into a microbial polysaccharide solution, further adding a cross-linking agent, introducing nitrogen for isolating air into a sealed system, adding an initiator, and reacting for 4-24 hours at the constant temperature of 30-60 DEG C, thereby preparing the gel plugging agent. The method has the advantages that firstly, the gelling time and the gel strength are controllable; secondly, injection risk is prevented, pipelines and shafts are not plugged, the plugging agent has pseudoplastic property, and after the gel is filled into an underground layer, the original strength can be still recovered after a pump is stopped and the shaft is closed; thirdly, large-scale treatment can be performed, the gel can be filled into a deep part of a stratum and is not greatly retained near the shaft bottom to limit the entering depth; fourthly, relatively high strength is achieved, high temperature oil deposit of 70-130 DEG C is adapted, and technical guarantee is provided for exploration of the high temperature oil deposit; and fifthly, the mineralization degree is 25*10<4>mg / L, so that the gel is relatively high in strength and is applicable to high-salt oil deposit.

The present invention provides a controlled release device for sustained or pulsatile delivery of pharmaceutically active substances for a predetermined period of time. This invention further provides such device in which sustained or pulsatile delivery is obtained by the unique blend and intimate mixture of pharmaceutically active substance with a microbial polysaccharide and uncrosslinked linear polymer and optionally a crosslinked polymer and / or lipophillic polymer and / or saturated polyglycolyzed glyceride. The invention also provides a process for the manufacture of such devices and pharmaceutical compositions containing the same.

The invention discloses a purely physical preparation method of vegetal and microbial polysaccharides. The purely physical preparation method comprises the following steps of drying a sample, crushing the dried sample, sieving the sample powder by a sieve of 60 to 80 meshes, uniformly mixing the sample powder and water, pouring the mixture into a high pressure homogenizer, carrying out homogenization under certain pressure, carrying out centrifugation, collecting a supernatant, carrying out separation of the supernatant by an ultrafiltration membrane of which molecular weight cut-off is 2-5 times higher than molecular weight of vegetal or microbial active polysaccharides to obtain a penetrating fluid, carrying out separation of the supernatant by an ultrafiltration membrane of which molecular weight cut-off is 1 / 5-1 / 2 of molecular weight of the vegetal or microbial active polysaccharides to obtain a trapped fluid, concentrating the trapped fluid, and carrying out freeze drying to obtain an active polysaccharide sample. The purely physical preparation method has a high polysaccharide extraction ratio, short time consumption, protein-removal effects superior to those of the traditional sevage method, does not utilize any organic reagent in polysaccharide extraction, and has a low production cost and no pollution.

The invention discloses a medium-high temperature resistant biological profile modifying / water plugging agent for oilfield exploitation. The medium-high temperature resistant biological profile modifying / water plugging agent consists of a microbial polysaccharide base profile modifying agent and delay gel, wherein the microbial polysaccharide base profile modifying agent is a microbial polysaccharide curdlan compound hydrogen bond binding force accelerant or microbial polysaccharide curdlan and a coagulant aid; the delay gel is one or more of polyhydric alcohol, high-molecular polymer hydrolysis polyacrylamide and gluconate. The invention further discloses a preparation method for the biological profile modifying / water plugging agent. The profile modifying / water plugging agent disclosed by the invention can be used for keeping the mobility of the system at a medium-high temperature (65-90 DEG C); moreover, the viscosity is increased slowly, so that the needs of smoothly conveying the profile modifying / water plugging agent to a special underground depth part are met.

A preparation method of a soft capsule without gelatin belongs to the technical filed of processing of soft capsules. The preparation method is that pullulan taken as major ingredient is respectively mixed with xanthan gum, carrageenan or gellan gum and other microbial polysaccharide gum or seaweed polysaccharide gum, the mixture is subjected to such procedures as sol, degassing, drooling and drying to form a gum skin film, and the gum skin film is pressed through a soft capsule pressing machine via a film rotating method to form the soft capsule. According to the invention, the soft capsule only adopts the pullulan, xanthan gum or carrageenan and other microbial polysaccharide gum or seaweed polysaccharide gum as the raw materials, and does not use the gelatin, adopts two processes of drooling to form the film and soft capsule pressing through the film rotating method; and meanwhile, during pressing, any oil-soluble drugs or health care food can be installed, so that the soft capsule without gelatin can be made.

The invention relates to a preparation method of microbe polysaccharide Gellan gum belonging to the microbe fermentation production field. The method comprises steps of preparing the slope strains, preparing the seed liquid, inoculating the seed liquid into the fermentation culture medium to ferment. The invention solves the problems of high production cost, low product yield (the density of the Gellan gum coarse polysaccharide is 1.3g / 100ml) exiting in known technology. The invention employs starch as the carbon source for the second level expanding culture medium and fermentation culture medium, and has advantages of low cost, high yield. The density of the Gellan gum coarse polysaccharide is 1.5-1.6g / 100ml when the fermentation is finished.

The present invention discloses deproteinizing process of gullan gum fermenting liquid, and the process includes pre-treatment of the fermented liquid, deacylating treatment of the fermented liquid, deproteinizing enzymolysis treatment of the fermented liquid, and other steps. The present invention is used effectively in the post-treatment of the extracted microbial polysaccharide, and has the advantages of simple operation, mild condition, low power consumption, low material consumption, low extracting cost and other advantages.

The invention provides a nano selenium micromolecular microbial polysaccharide, which is prepared under ultrasonic condition by taking micromolecular Caragana rhizobia extracellular polysaccharide as a raw material through uniformly dispersing and co-acting with selenium dioxide and a reducing agent. The production method specifically comprises the steps of: preparing a micromolecular bacterial polysaccharide solution with a proper concentration, uniformly mixing micromolecular Caragana rhizobia polysaccharide with vitamin C, slowly dropwise adding selenium dioxide to the mixed system, carrying out ultrasonic dispersion and reaction, dialyzing and freeze-drying. The nano selenium micromolecular microbial polysaccharide product prepared by the preparing process has the selenium content up to 15.2-16.4mu g / mg, selenium average grain size about of 380 nm and polysaccharide yield more than70%. Mouse test shows that the nano selenium micromolecular polysaccharide has excellent anticancer activity, and can be applied to preparing anticancer medicaments and health products.

The microwave polysaccharide degrading process includes compounding polysaccharide solution of 5-150 g / L concentration and adding acid in concentration of 0.1-2.5 mol / L; setting the solution inside microwave generator and regulating power to 50-300 W to degrade at 10-90 deg.c for 1-30 min; and neutralizing pH to 7 with alkali, dialyzing to eliminate generated small molecular salt and freeze drying to obtain polysaccharide oligomer. The polysaccharide is originated from plant, animal and microbe and has numerically average molecular weight of 5-2000 KDa. The present invention has simple technological process, low cost and less pollution. The said technological process makes it possible to obtain various polysaccharide in specific molecular weight section and with greatly raised clinical bioavailability.

The invention discloses epidermal growth factor compound lipidosome as well as a preparation method and application thereof, and belongs to the field of traditional Chinese medicines. The epidermal growth factor compound lipidosome is prepared by the following raw materials in parts by mass: 0.1-5 parts of epidermal growth factors, 5-30 parts of silibinin, 5-30 parts of coix seed oil, 5-30 parts of yeast extracts, 5-30 parts of microbial polysaccharide, 30-200 parts of hydrogenated lecithin, 10-80 parts of cholesterol and 5-30 parts of glycerinum. The epidermal growth factor compound lipidosome has the advantages of simple technique, safety, no poison, little skin irritation, mild property and stable effect.

The present invention belongs to microbial polysaccharide fermentation, and particularly relates to a method for producing a microbial polysaccharide fermentation broth by using Paenibacillus mucilaginosus, wherein Paenibacillus mucilaginosus is inoculated in a culture medium containing a carbon source and a nitrogen source to carry out fermentation, and bacteria heat stimulation, addition of a surfactant during a fermentation process, and other manners are performed to optimize the production process. With the present invention, the problem of low yield of polysaccharides in the fermentation broth in the prior art is solved, and advantages of high polysaccharide yield in the fermentation broth, good bacterial growth, short fermentation period and the like are provided.

A process is described for the production of an immunostimulant by submerged cultivation of Lentinus edodes in which mycelium from agar plates or a fermentation broth is added to a liquid medium in a shake flask or a bioreactor containing nutrients such as malt extract, yeast extract, peptone and glucose having access to air or to which air is added, and which is kept in constant movement at approx. 28° C. At the proper conditions, there will be an increase in the production of extracellular lentinan, which is shown to be a better immunostimulant than intracellular lentinan. The extracellular product is precipitated from the growth medium by means of methods for the precipitation of microbial polysaccharide.

The invention discloses a high-efficiency phosphate solubilization plant growth-promoting bacterial agent. The high-efficiency phosphate solubilization plant growth-promoting bacterial agent is characterized by comprising an aspergillus niger C2 bacterial agent, an additive, a binding agent and a protective agent, wherein the aspergillus niger C2 bacterial agent comprises aspergillus niger C2 with a preservation number of CGMCC NO.7511; the additive is one or more than one of silicon dioxide, kaolin, rice hull powder, wheat hull powder and straw powder; the binding agent is bentonite or water soluble microbial polysaccharide; the protective agent is one or more than one of glycerol, skimmed milk, plant oil, sodium alginate and chitosan. The high-efficiency phosphate solubilization plant growth-promoting bacterial agent is significant for saving phosphatic fertilizer resources for agricultural production in China, continuously increasing yield of grain and protecting farmland ecological environment.

The invention belongs to post-extraction of microbial polysaccharide, and in particular relates to a post-extraction method of curdlan. The method comprises the following process steps: fermentation broth enzyme treatment, membrane treatment, first dissolution, sedimentation and filtration, secondary dissolution, sedimentation and filtration, drying and the like. By using the method of the invention, the technical defects in the prior art that high production cost is high because of the adoption of alcohol extraction and large potential safety hazard exists in the production process are solved. The post-extraction method of the invention can be used for effectively removing thallus and impurities and has the advantages of rapid extraction schedule, small influence on material quality, safe production and low cost.

The invention relates to a preparation method of a gelatine-free double-layer hollow capsule. The preparation method is characterized by comprising the following steps of: by taking microbial polysaccharide gum, algal polysaccharide gum and plant polysaccharide gum as raw materials; and mixing, dissolving the gums, degassing, dipping the gums, drying, dipping the gums again, drying again, shelling, cutting and trimming, so as to prepare the gelatine-free double-layer hollow capsule, wherein the inner layer of the capsule contains pullulan and sodium alginate, and the outer layer of the capsule contains hydroxypropyl methyl cellulose and methylcellulose. With the adoption of the double-layer structure, the disintegration performance of the capsule can be greatly improved, and the good mechanical strength of the whole capsule can be kept. The product can be used for filling various medicines and healthcare foods by taking the microbial polysaccharide gum, the algal polysaccharide gum and the plant polysaccharide gum as the raw materials and avoiding the use of any gelatine ingredient; the product is safe, reliable, and is suitably eaten by Buddhist, Moslems and vegetarian; and the worry of people about eating for gelatine products caused by 'bovine spongiform encephalopathy' is avoided.

The invention relates to the technical field of microbial polysaccharide extraction, and particularly discloses a method for extracting Sanzan gum from fermentation liquor and a product thereof. The method for extracting Sanzan gum from the fermentation liquor comprises the following steps: treating the fermentation liquor with neutral soluble salt, adding ethanol to enable the concentration of ethanol in the system to reach 30-40%, adjusting the pH value of the system to 1.0-3.0, carrying out separation operation, soaking the obtained crude product with ethanol to obtain a material system with the ethanol concentration of 72-85%, and then adjusting the pH value of the material system to 6.0-8.0, ensuring that the concentration of ethanol in the material system is 70-82%, and separating again. The product obtained by the invention has the advantages of high gel strength, high product viscosity, low total number of bacterial colonies, high product percent of pass and the like. Meanwhile, the obtained mother liquor can be recovered and reused after being distilled, so that the method has very high practicability and is beneficial to industrial production.

The invention relates to a biology process method for paper making black liquid. The invention aims at the alkali of paper making black liquid and hard degradability of the lignin, using acid-producing white rot bacterium to produce organic acid to taking acid out of lignin in the paper making black liquid, deceasing alkaline and taking off lignin to make effluent water whose PH value is between 2 to 3. Acidity effluent water would decrease the content of acid soluble lignin and COD to produce effluent water that COD is below 2000 and PH value is from 5 to six. The effluent water comes to anaerobic and aerobiosis system to realize the standard. And the draft can be used for developing microorganism and organic fertilizer. The invention discloses suited to many kinds of paper making factory, especially for middle and small factory to effectively solve the pollution problem.

The invention discloses a system device for strengthening high-viscosity microbial polysaccharide fermentation. The system device comprises a tank body, a tank cover, a cooling interlayer, an air distributor, a first motor, a second motor, a supergravity revolving bed device and a high-temperature-resisting submerged pump. A rotary shaft is longitudinally arranged inside the tank body. The upper end of the rotary shaft penetrates through the tank cover to be in driven connection with the first motor, and the lower end of the rotary shaft extends into the tank body to be connected with the supergravity revolving bed device. The supergravity revolving bed device is transversely arranged at the center of the inside of the tank body. The air distributor is arranged at the lower end of the supergravity revolving bed device and is an annular pipeline. The high-temperature-resisting submerged pump is arranged on one side of the lower end of the inside of the tank body. One end of the high-temperature-resisting submerged pump is connected with the supergravity revolving bed device through a pipe, and the other end of the high-temperature-resisting submerged pump is connected with a rotary shaft of the second motor. The cooling interlayer is arranged outside the tank body. A fermentation period is greatly shortened by strengthening dissolved oxygen mass transfer, gas, liquid and solid phase mixing, rotating speed optimization, ventilatory capacity and other conditions, the yield and mass of high-viscosity polysaccharide are increased, and the cost is remarkably reduced.

The microorganism polysaccharide is made with the CCTCC M 205108 Chinese peashrub rhizobium as bacterial, using the carbohydrate as carbon source, and using ammonium salt as nitrogen source. The method comprises the following steps: culturing the slant face seed, culturing shaking bottle seed, culturing seed jug, batch fermentation or supplying material batch fermentation, and extracting polysaccharide. The output of batch fermentation is 7.7g / L-10.2g / L, and the output of supplying material batch fermentation is 15.1g / L-16.2g / L. The molecular weight of the polysaccharide is between 2.7X104Da and 3.0X104Da. According to mouse test, the polysaccharide possesses the action of improving immunity.

The invention provides a fermentation method of a microbial polysaccharide flocculant, comprising the steps of choosing arthrobacter LF-Tou2 as a producing strain of the microbial polysaccharide flocculant and adopting cascaded strain propagation and fermentation methods to produce the high-efficient microbial polysaccharide flocculant used for water quality purification. The microbial polysaccharide flocculant of the invention is non-toxic and biodegradable, has the advantages of low production cost, wide application range, high service efficiency and no secondary pollution when being used, has comprehensive functions of removing inorganic ions, reducing contents of organic matters and adsorbing heavy metals, is applied in such technical fields as purification of quality of supplied water, treatment of inland lake water and sewage quality, separation of biological products in the fermentation industry and the like, and has very obvious economic and social effects.

The invention belongs to the technical field of tertiary oil recovery, particularly relates to a method for microbial flooding by using a microbial polysaccharide system. The method includes the following steps of screening of oil deposits, screening of a microbial polysaccharide activation agent system, determination of the on-site injection amount and injection process of the microbial polysaccharide activation agent system, execution of a field test and evaluation of the field-test effect. The method has the advantages that the process is simple and highly targeted, the field-test effect isgood and the input-output ratio is high, the input-output ratio is larger than 1:6, the comprehensive water decrease rate is larger than 10%, and the recovery ratio is larger than 15%. The method iseconomical and effectively improves the oil reservoir rate, and the method can be widely used in the technical field of microbial oil recovery.

The invention discloses a biological modifying and flooding agent capable of resisting against high temperature and high salt for oil field exploitation. The biological modifying and flooding agent is prepared from the following raw materials by weight percent: 0.01-5% of microbial polysaccharide, 0.05-10% of surfactant, 0.01-10% of binding force accelerator and the balance of water. The biological modifying and flooding agent disclosed by the invention is capable of forming lower interfacial tension with the crude oil, has relative stable viscosity and can keep higher involving rate under alkali-free, high-temperature and high-salt conditions; the application scope is wide; the operability is simple and convenient; the use cost of the user is lowered; and the environmental pressure is reduced.

The invention discloses a sphingol pseudoalteromonas genetically engineered bacterium and relates to sphingol pseudoalteromonas which stress resistance gene is guided into. The stress resistance geneis from extreme microorganisms. The invention further discloses a method for constructing the sphingol pseudoalteromonas genetically engineered bacterium through a three-parent combining method. The invention further discloses application of the sphingol pseudoalteromonas genetically engineered bacterium in fermentation to prepare welan gum or gellan gum. The sphingol pseudoalteromonas geneticallyengineered bacterium disclosed by the invention obviously improves temperature tolerance and acid-base tolerance of the sphingol pseudoalteromonas, a temperature tolerance range is 30 to 42 DEG C, and a pH tolerance range is 4.0 to 10.0. When the sphingol pseudoalteromonas genetically engineered bacterium is used in fermentation to prepare the welan gum, a microbial polysaccharide yield can reach20 to 30g / L under the condition that a pH is not regulated by an external source; when the sphingol pseudoalteromonas genetically engineered bacterium is used in fermentation to prepare the gellan gum, a microbial polysaccharide yield can reach 15 to 22g / L under the condition that a pH is not regulated by an external source; a production efficiency of microbial polysaccharides of the gellan gum,the welan gum and the like is enhanced.

The invention relates to microbial fermentation, in particular to a method for producing microbial polysaccharide fermentation liquor by using waste glucose mother liquor. In the method, sphingomonas CGMCC No,1650 is used as a productive strain, and the strain is subjected to expanding culture and then inoculated to a fermentation culture medium for fermentation, wherein the preprocessed waste glucose mother liquor is used as a carbon source for the fermentation culture medium. The invention overcomes the defects of huge raw material waste, high production cost, difficulty in environmental-protection and the like in the prior art. The method achieves the advantages of simple process, easiness for implementation, low manufacturing cost, better adaptability to an industrial policy in China in technological advancing direction, and the like on the premise of ensuring that product quality is equivalent to that of the traditional process.

The invention belongs to the technical field of industrial production of curdlan gum, and particularly relates to a curdlan gum purification method and application, aiming to solve the problems of microbial polysaccharide curdlan gum in the prior art, including low sterilizing efficiency and poor sterilizing effect of fermentation liquid, low product purity, low gel transparency, high ion concentration, and low strength of formed gum (based on GB28304). According to the high-efficiency curdlan gum fermentation liquid sterilization method, the curdlan gum fermentation liquid or crude curdlan gum products are dissolved in alkali liquor, the viscosity is quickly reduced through high shear, a filter is used for efficiently filtering out bacteria and insoluble impurities, acids are added to supernatant for neutralization and sedimentation to obtain curdlan gum, and the curdlan gum is subjected to filtration and concentration, wash desalting, ethyl alcohol sedimentation and drying to obtain a high-quality curdlan gum product. The method is simple and convenient to operate, economical, and applicable to large-scale industrial production of the high-quality curdlan gum.

The invention provides a method for extracting microbial polysaccharide flocculant by membrane concentration and a pneumatic drying method. The method comprises the following steps: the filtering, the separation concentration, the deposit carboxylation and the drying are performed to the arthrobacter LF-Tou2 of a microbial polysaccharide flocculant producing strain through fermenting liquor generated by fermentation, and then the microbial polysaccharide flocculant is prepared. Compared with the prior art, the method has the advantages of high preparation efficiency, high purity and high activity of the products and no secondary pollution during the use; and the method is suitable for the industrial production as well the wide popularization and application, especially for the processing and the application of the raw water of the drinking water and has board application prospect.