The preparation method of the composite
soybean protein gel comprises the following steps: adjusting the pH value of an
egg white protein solution, carrying out heat preservation, adding
protein glutaminase to carry out
hydrolysis reaction, centrifuging, collecting supernate, and freeze-
drying to obtain deamidated
egg white protein; adding TG
enzyme into the
soybean protein isolate and deamidated
egg white protein solution for enzymatic cross-linking reaction, centrifuging after
enzyme deactivation, collecting supernate, and freeze-
drying to obtain compound protein
powder; dissolving the composite protein
powder in water, stirring, and heating to form composite
soybean protein gel; according to the preparation method disclosed by the invention,
glutamine in the egg white protein is hydrolyzed by using the protein
glutaminase, so that only the site of
lysine is reserved, and then TG
enzyme treatment is performed, so that the crosslinking of the egg white protein is avoided, and the content of disulfide bonds in the soybean protein is increased, thereby remarkably improving the
gel strength and compression resistance of the composite soybean protein thermal gel.