1477 results about "Natural product" patented technology

The invention relates to the field of natural products, in particular to a dimer compound of guaiane type sesquiterpenes and uric acid as well as an extraction and separation method and application of the dimer compound. According to the invention, a new dimer compound of guaiane type sesquiterpene and uric acid is extracted and separated from carpesium abrotanoides in Guizhou, and it is found that the dimer compound has relatively strong anti-tumor activity. The compound has strong inhibitory activity on breast cancer, prostate cancer, nasopharynx cancer, liver cancer, lung cancer, leukemia, pancreatic cancer, glioma, osteosarcoma, skin cancer, cervical cancer, ovarian cancer, kidney cancer and esophageal cancer, and provides an optional scheme for broad-spectrum anti-cancer drugs. Moreover, in human prostate cancer cells PC3, human cervical cancer cells Hela, human liver cancer cells HepG2 and human glioma cells U87 which are resistant to paclitaxel, the compound also shows strong anti-cancer activity, which indicates that the compound has the potential of treating patients resistant to paclitaxel.

The invention discloses a probiotic-polyphenol nanoparticle colon-targeted co-delivery system as well as a preparation method and application thereof, and belongs to the field of biological medicines. The FCPs and hyaluronic acid (HA) are combined through non-covalent interaction, so that the hydrogel has colon targeting property and mucosal adhesion at the same time, and delivery of probiotics is facilitated. The hydrogel (HF) based on polysaccharide has good biocompatibility, and can be used for effectively encapsulating the probiotics and the natural products. Then, the PDA-TH NPs and BA are incorporated into the polysaccharide chain network of HF, and finally the BA-HF-PDAT targeted co-delivery system is constructed. The BA (at) HF-PDAT targeted co-delivery system can protect BA from serious gastrointestinal stress, and is jointly assembled with PDT-TH NPs to realize dual slow release of thymol (Thy), so that the treatment effect of BA and Thy is improved, and the BA (at) HF-PDAT targeted co-delivery system contributes to treatment of clostridium difficile infection (CDI).

The invention discloses a compound with anti-tumor activity. The compound has a chemical structural formula as shown in a formula (I). The compound belongs to a novel tetracyclic triterpenoid natural product, has good fat solubility and chemical stability, and can be conveniently extracted from the rhizome of a herbal medicine Polygala fallax Hemsl. And prepared into an oral or injection form. The invention also provides a preparation method and application of the compound. The compound provided by the invention can obviously inhibit the growth of mouse lung cancer and reduce the volume and weight of tumor, and can be used for treating tumor diseases.

The invention discloses a method for extracting and purifying flavone in litsea coreana through an ultrasonic-assisted eutectic solvent, and belongs to the technical field of green processing of natural products. The invention provides a method for efficiently preparing litsea coreana flavone by integrating computer prediction, green solvent extraction and resin purification technologies. The method comprises the following steps: firstly, calculating and screening a deep eutectic solvent system with the highest affinity with the litsea coreana flavone on the basis of a COSMO-RS theoretical model, then coupling the optimized DES with an ultrasonic-assisted extraction (UAE) technology, and through the synergistic effect of the DES and the UAE technology, remarkably improving the extraction rate of the litsea coreana flavone and furthest keeping the biological activity of a target component; then, a macroporous resin dynamic adsorption-desorption purification process is linked, so that high-selectivity enrichment and impurity removal of the flavonoid compounds in the crude extract are realized, a high-purity product is obtained, and a complete process route from intelligent screening, efficient extraction to precise purification is finally formed.

The invention belongs to the field of natural products, and discloses camellia oleifera shell polysaccharide as well as a preparation method and application thereof. The structural formula of the camellia oleifera shell polysaccharide is # imgabs0. The camellia oleifera shell polysaccharide of some examples is low in molecular weight, better in thermal stability, good in anti-inflammatory activity and free of toxicity to cells.

The invention relates to the technical field of plant effective component extraction, in particular to a deep-eutectic solvent and application thereof, and the deep-eutectic solvent is composed of anhydrous betaine, DL-malic acid and deionized water. The eutecticevaporate solvent is applied to extraction of momordica grosvenori polysaccharide, ultrasonic waves are combined with the eutecticevaporate solvent, in addition, extraction conditions of crude momordica grosvenori polysaccharide are optimized, and a new method is provided for efficient and green extraction of momordica grosvenori polysaccharide. The new method not only expands the application range of DESs in natural product extraction, but also provides important technical reference for industrial development of momordica grosvenori polysaccharide.

The invention discloses pancreatic lipase immobilized composite nano magnetic beads, a preparation method and application, ferroferric oxide is adopted as a magnetic core, dopamine-mediated growth is performed to form a core-shell structure, then the periphery of the core-shell structure is wrapped with a metal organic framework to form the composite nano magnetic beads, and pancreatic lipase is immobilized by using the composite nano magnetic beads as an adsorption carrier. The enzyme loading amount, the repeated utilization rate and the storage stability of the ferroferric oxide nano-particles are remarkably improved, the enzyme immobilization efficiency and the enzyme activity are improved, and meanwhile, the prepared pancreatic lipase immobilized composite nano-magnetic beads have excellent stability and biocompatibility, are green and pollution-free and can be repeatedly used; the composite nano magnetic beads are synthesized by adopting a solvothermal method, the product is easy to separate, the synthesis process is simple, the enzyme immobilization efficiency can be regulated and controlled by regulating and controlling the synthesis process, the structure of an active ligand can be screened and analyzed from natural products with numerous and jumbled components, and the active ligand has a certain lipid-lowering potential and can be used for preparing the nano magnetic beads. The compound can be used as a potential lead compound of lipid-lowering drugs.

The invention belongs to the field of natural product extraction, and relates to a method for extracting and separating flavonoid compounds from Artemisia chrysantha, which comprises the following steps: carrying out reflux extraction on the dry overground part of Artemisia chrysantha by using 70% ethanol, loading to a balanced D101 macroporous adsorption resin column, sequentially eluting by using water, a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution, and collecting the eluent. Four fractions from Fr. I to Fr. IV are obtained; respectively carrying out polyamide column chromatography separation on Fr.II to Fr.IV, and sequentially eluting with a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution to respectively obtain three fractions; and further purifying the Fr.II-1, the Fr.II-2, the Fr.III-1, the Fr.III-2, the Fr.III-1 and the Fr.III-3 by virtue of a Sephadex LH-20 column, so as to finally obtain seven flavonoid compounds. According to the method, only the ethanol water low-toxicity solvent is used for extraction and preparation, organic solvents with high toxicity are not used, the method is green and environment-friendly, the extraction efficiency is high, the number of extracted compounds is large, chemical components of the artemisia europaea medicinal material are enriched, the resource source of active components is widened, and a foundation is laid for follow-up research.

To provide a new composition consisting of 100% natural matter, exerting a therapeutic or beneficial effect in the treatment of bone fragility, having a physiological mode of action.SOLUTION: The present invention provides a 100% natural product consisting of natural matrices exhibiting emerging properties, which enables re-establishing correct bone metabolism by correcting behavior of mesenchymal stem cells and re-establishing a correct balance between cell populations that allow bone growth or remodeling. The natural product consists of, for example, Equisetum flowering tops, Acacia, Malpighia fruit, coral skeleton, avian eggshell, Agave leaves, Cetraria stems, Agaricus, and calcium citrate.SELECTED DRAWING: None

The invention belongs to the field of microbial natural product pesticides, and particularly discloses a polyketone compound as well as a preparation method and application thereof. The compound disclosed by the invention is prepared from a fermentation crude extract of a strain of tobacco endophytic fungus Aspergillus japonicus TE-739D, and the compound disclosed by the invention is prepared from the fermentation crude extract of the tobacco endophytic fungus Aspergillus japonicus TE-739D. The chemical structure of the compound is identified through a modern spectroscopy technology, such as a high resolution mass spectrum HRESIMS and a superconducting nuclear magnetic resonance spectrum NMR, and the compound is named as japonidiene C. The invention further discloses a preparation method of the compound. The polyketone compound japondiene C has an obvious insecticidal activity on 2-instar larvae of spodoptera frugiperda, and can be used for preventing and treating the second instar larvae of the spodoptera frugiperda.

The invention discloses a method for extracting eucommia ulmoides with an intestinal repair function, and relates to the technical field of natural product extraction.The method comprises the steps that a fresh eucommia ulmoides raw material is subjected to low-temperature drying and smashing, a polysaccharide water extract and filter residues are obtained through water extraction, and then the flavone residual quantity in the polysaccharide water extract is synchronously detected; carrying out enzymolysis on the polysaccharide water extract through beta-glucanase to obtain a small molecular polysaccharide solution, and carrying out alcohol extraction on filter residues to obtain a flavone alcohol extract; the preparation method comprises the following steps: mixing the two components according to a ratio, inoculating compound probiotics for anaerobic fermentation, regulating according to the residual quantity of flavone, regulating an alcohol extraction process by using short-chain fatty acid generated by fermentation when the residual quantity is high, supplementing flavone extract in an enzymolysis system when the residual quantity is low, and finally removing impurities, purifying, adapting intestinal tracts and drying to obtain the product. According to the method, regulation and control operation is correspondingly executed by detecting the residual quantity of flavone in the polysaccharide water extract, so that self-supply of a regulation and control reagent and high efficiency of active ingredients are realized, the conversion rate of small molecular polysaccharide and flavone aglycone is increased, and the intestinal tract tolerance of the product is optimized.

The invention discloses a limonin compound and application thereof in preparation of a medicine for preventing and / or treating cancers. The structure of the limonin compound is shown as a formula I or II. The cancer is at least one of breast cancer, liver cancer, gastric cancer, esophageal cancer, leukemia, pancreatic cancer, glioma, osteosarcoma, prostatic cancer, nasopharynx cancer, cervical cancer, ovarian cancer, kidney cancer and skin cancer. According to the invention, activity screening is carried out on various natural product extracts, it is found for the first time that limonin compounds with structural formulas I and II have excellent inhibitory activity on various different types of human cancer cells, and an optional scheme is provided for broad-spectrum anti-cancer drugs. Related results also show that the compound provided by the invention can inhibit migration and invasion ability of breast cancer cells, promote cell apoptosis and retard a cell cycle. The limonin compound applied in the invention is derived from mallotus oblongifolius, the source is wide, and the production cost is low.

The invention discloses a liver protection gastrodia elata polyphenol extract as well as a preparation method and application thereof, and belongs to the technical field of natural product extraction. The method aims at solving the problems that in the prior art, gastrodia elata polyphenol extraction efficiency is low, and active ingredients are prone to being damaged by heat. According to the technical scheme, the method is characterized by comprising the following steps: mixing a gastrodia elata raw material with liquid nitrogen after being subjected to freezing and unfreezing circulating pretreatment, and performing cell wall breaking treatment through a high-voltage pulsed electric field; mixing the wall-broken raw material with a buffer solution, carrying out enzymolysis by virtue of an immobilized enzyme reactor, cooling the system after enzymolysis, and carrying out ultrahigh-pressure extraction; centrifugally filtering the extracting solution, purifying through a macroporous adsorption resin column, and collecting ethanol eluent; and finally, carrying out vacuum concentration and spray drying on the eluent to obtain the gastrodia elata polyphenol extract. According to the method, the polyphenol yield is effectively improved, the activity of the components is protected, and the obtained extract can be used for preparing liver protection medicines.

Disclosed are modified Type 1 O-methyltransferases (OMTs) and methods of using such modified OMTs. In some forms of the modified OMTs, the amino acid corresponding to Phe337 of Sorghum bicolor stilbene OMT (SbSOMT) is substituted with a polar amino acid. In some forms of the modified OMTs, the amino acid corresponding to Asn323 of Sorghum bicolor caffeic acid OMT (SbCOMT) is substituted with a hydrophobic amino acid. In some forms of the modified OMTs, the amino acid corresponding to Ile144 of SbSOMT is substituted with a polar amino acid. In some forms of the modified OMTs, the amino acid corresponding to Asn128 of SbCOMT is substituted with a hydrophobic amino acid.

Provided is a mammalian-derived cell model that can be used in anti-aging drugs. The mammalian-derived extraembryonic trophoblast cell model is an early mammalian-derived extraembryonic stage. A change model and an aging evaluation index system for differentiating the cells into the extraembryonic trophoblast cells / organoids can be used for various anti-aging researches and target detection, anti-aging drug detection, screening and identification, research on innovative drugs / natural products, and development of chemicals and / or health foods and additives. Screening and identifying many resistances is helpful. Candidate targets of aging may be further mechanically and functionally validated for future therapeutic use. The screening of anti-aging drugs or natural products will produce huge economic and social benefits.

The invention discloses a bifunctional flavin adenine dinucleotide synthetase mutant and an application of the bifunctional flavin adenine dinucleotide synthetase mutant in FAD synthesis. The mutant disclosed by the invention is RFK / FADSC18R or RFK / FADSC18R-R46H, and the structural formula of the mutant is as shown in the specification. The amino acid sequence of the RFK / FADSC18R is as shown in SEQ ID NO. 15, and the amino acid sequence of the RFK / FADSC18R-R46H is as shown in SEQ ID NO. 16. A genetically engineered bacterium carrying the bifunctional flavin adenine dinucleotide synthetase mutant gene plasmid is used for producing flavin adenine dinucleotide by using glucose as a substrate through a simple fermentation process under a shake flask aerobic condition, and the FAD yields of strains XF02 and XF03 containing mutant genes are 79.31 mg / l and 167.56 mg / l in sequence; and compared with a strain XF01 containing a non-mutated gene, the mutant gene is improved by 58.62% and 235.12% respectively. A microbial'cell factory 'is constructed through a metabolic engineering strategy, and the modified microorganisms can generate required natural products in microbial cells by utilizing low-price raw materials. The progress not only reduces the production cost of the FAD, but also improves the environmental sustainability of the production process of the FAD.

The present invention provides an essence containing chlorella extract and a preparation method thereof. The chlorella extract process comprises the following steps: raw material pulverization; enzymatic hydrolysis with cellulase and trypsin; primary fermentation using a mixture of Bacillus natto and Bacillus pumilus; addition of bamboo leaf extract, corn steep liquor, and sucrose, followed by inoculation with Rhodotorula glutinosus for secondary fermentation to obtain the chlorella extract. The mixed fermentation of multiple strains exhibits a synergistic effect, resulting in greater enrichment of natural products during the fermentation process compared to single-strain fermentation. Furthermore, the present invention provides an essence containing chlorella extract, glycerin, sodium hyaluronate, honey, and water, which exhibits significant moisturizing, antioxidant, and soothing effects.

The invention belongs to the technical field of natural product extraction, and particularly relates to a research method for extracting polyphenol and flavonoid compounds in peony petals through an electrochemically-assisted eutectic solvent. The invention provides a research method for extracting polyphenols and flavonoids compounds in peony petals by using an electrochemically-assisted deep eutectic solvent, electrochemical wall breaking and DES regulation are combined for the first time by using a solvent design-electrochemical activation-targeted extraction method, phenol oxidation caused by traditional heating is avoided, and meanwhile, the extraction efficiency of the polyphenols and flavonoids compounds in peony petals is improved. The dissolution of target components is selectively enhanced through electrode reaction, the extraction efficiency of bioactive components in the peony petal powder is effectively improved, and the method has the advantages of greenness, high efficiency, short-time consumption reduction and the like, overcomes the defects in the prior art, and has a good application prospect.

To provide a method for determining when a natural therapeutic or beneficial product will exert its therapeutic or beneficial effect via a physiological mechanism of action.SOLUTION: The present invention relates to a new method that allows the evolution of medical technologies from the use of chemically or biologically defined artificial substances to self-assembling natural products obtained from natural raw materials by industrial processes that preserve their endogenous properties, thereby preserving their ability to interact with the networks of the living world (including humans), which natural products cannot be defined with classical qualitative and quantitative composition schemes. Thus, the present invention provides a new method for determining when a therapeutic or beneficial product exerts its therapeutic or beneficial effect via a physiological mechanism of action. This method provides the tools necessary for the skilled person to assess the mechanism of action of a therapeutic or beneficial product, which has become a relevant feature to assess with new developments in regulatory regimes for medical devices and nutraceuticals, for which there is no method available in the art.SELECTED DRAWING: None

The invention discloses a preparation method of an aronia extract and an application of the aronia extract in protecting an intestinal barrier function, and belongs to the technical field of natural product extraction. The method comprises the following steps: mixing a hydrogen bond acceptor and a hydrogen bond donor according to a molar ratio of 1: (1-3), heating, and continuously stirring to obtain a natural eutectic solvent; dissolving an enzyme in the natural eutectic solvent to obtain an enzyme solution; mixing the enzyme solution with the aronia fruit powder, and performing ultrasonic extraction, enzyme deactivation and purification to obtain the aronia extract. According to the method, a hydrogen bond donor in a natural eutectic solvent is comprehensively utilized to improve the stability and solubility of anthocyanin in a system, and the good regulation and control capability and molecular complexing stability of hydrogen bond receptor natural quaternary ammonium salt; the method is superior to a traditional method in the aspects of extraction rate, environmental friendliness and raw material availability, the content of the extracted effective components is increased by nearly two times or more, and the method is suitable for high-value utilization of anthocyanin-rich natural resources represented by aronia.

The invention discloses application of tea extracellular vesicles in preparation of a product for preventing and / or treating UVB-induced skin photoaging, and belongs to the technical field of application of the tea extracellular vesicles. The tea extracellular vesicles are derived from tea leaves and / or stem leaves and are prepared by a method of combining enzymolysis, multi-stage filtration and ultracentrifugation, and the particle size of the tea extracellular vesicles is mainly distributed in a range of 60-100nm. Experiments show that the tea extracellular vesicles can effectively reduce active oxygen accumulation and lipid peroxidation caused by UVB radiation, repair mitochondrial membrane potential and inhibit expression of matrix metalloproteinase and inflammatory factors, so that photoaging symptoms such as skin wrinkles, erythema and barrier function damage are relieved. The tea extracellular vesicle provided by the invention has the advantages of natural raw materials, good biocompatibility, small side effect and the like, can be used for preparing a plurality of external dosage forms such as gel, cream, spray and the like, and provides a safe and effective new natural product choice for prevention and treatment of skin photoaging.

The invention discloses a synthesis method of a novel benzosuberone compound, and belongs to the field of organic synthesis. The preparation method comprises the following steps: uniformly stirring methyltriphenylphosphonium bromide in a solvent at room temperature, dropwise adding an n-hexane solution of n-butyllithium in an ice-water bath, stirring a reaction system at room temperature for 30-40 minutes after dropwise adding is completed, then adding an initial raw material phenanthrenequinone 1 in the ice-water bath, transferring the reaction system to 40 DEG C for reaction, and after the reaction is monitored to be complete by a TLC (Thin Layer Chromatography) dot plate, filtering, washing and drying to obtain the phenanthrenequinone 1. And pouring the reaction liquid into a silica gel chromatographic column, and simply filtering by using dichloromethane as an eluent to remove salt and phosphine oxide. And performing rotary evaporation to remove the organic solvent to obtain a crude product, and separating and purifying to obtain a target product 2. The method is short in reaction time and low in reaction cost, provides a practical method for obtaining the seven-membered rigid ring skeleton, and has potential value for synthesis of natural products and bioactive molecules.

This invention belongs to the field of natural product extraction technology, specifically relating to a method for extracting total polyphenolic active substances from Gastrodia elata in food and cosmetics. The method uses a eutectic solvent as the extraction solvent, combined with ultrasonic extraction. The hydrogen bond acceptors of the eutectic solvent include polyethylene glycol-200, polyethylene glycol-400, and Tween 80, while the hydrogen bond donors include ethylene glycol, 1,2-propanediol, glucose, glycerol, and xylitol. This invention, using a eutectic solvent combined with ultrasonic extraction, achieves high extraction efficiency of total polyphenolic substances from Gastrodia elata, reaching a maximum of 33.2 mg / g. The extraction method of this invention is simple, has a short extraction time, and is practical, showing great development potential in the fields of medicine, food, and cosmetics.

The invention relates to the technical field of natural product extraction, and provides an extraction process for optimizing bougainvillea speetabilis bract total flavonoids by a response surface method.A bougainvillea speetabilis bract raw material is received and pretreated, a constant-temperature reflux and ultrasonic-assisted extraction technology is adopted, and Box-Behnken design in the response surface methodis combined, so that the bougainvillea speetabilis bract total flavonoids are obtained. Multi-factor interaction analysis is carried out on ultrasonic time, ethanol concentration and a material-liquid ratio, and a second-order polynomial regression model is established to predict an optimal extraction condition. According to the optimized extraction parameter combination, the ultrasonic time is 13.7 + / -0.5 min, the ethanol concentration is 60.0 + / -2.0%, the material-liquid ratio is 1: 29.7 + / -1.0, and under the conditions, the total flavone extraction rate can reach 8.48% or above. According to the method, the extraction efficiency of the bougainvillea speetabilis bract total flavonoids is remarkably improved, the process stability and the resource utilization efficiency are enhanced, meanwhile, the method has good industrial production suitability and technical innovation, and a new method is provided for extraction and utilization of the bougainvillea speetabilis bract total flavonoids.

The invention discloses a mulberry leaf extract for reducing body weight based on blood sugar regulation and a preparation method thereof, and belongs to the technical field of natural product extraction. The mulberry leaf extract is mulberry leaf DNJ, and the extraction and preparation steps of the mulberry leaf extract comprise pretreatment, composite enzymatic hydrolysis, ultrafiltration purification, resin adsorption and freeze-drying molding. By optimizing enzymolysis conditions and a separation and purification process, the extraction rate (gt; and therefore, a foundation is laid for industrial production. DNJ reduces blood sugar and body weight by promoting secretion of GLP-1, when DNJ is combined with GLP-1RA for use, a remarkable synergistic effect is shown, and the effects of reducing blood sugar and losing weight are further enhanced. The invention provides a natural and safe blood glucose reducing and weight losing active component and a preparation method thereof, provides a novel solution for treating diabetes and obesity, and has important social and economic values.

The invention discloses a self-assembled PROTAC nano material based on natural product mitochondria targeting, which is a nano particle prepared from mitochondria targeting molecules, a photosensitizer and PROTAC as raw materials through a nano precipitation method. The mitochondrial targeting molecule is berberine, the photosensitizer is hypericin, and the PROTAC is dBET57; the ratio of the amount of substance of the dBET57 to the amount of substance of the berberine is (1-10): (1-10), and the ratio of the amount of substance of the dBET57 to the amount of substance of the hypericin is (1-10): (1-10). The nano material can be used for blocking multiple energy metabolism pathways of tumor cells, including glycolysis and oxidative phosphorylation, meanwhile, mitochondria is damaged, ferroptosis is induced, and the anti-tumor capacity of pharmacodynamic molecules is improved; bRD4 in tumor cells and downstream carcinogenic protein c-Myc of the BRD4 can be efficiently degraded. The invention also discloses an application of the nano material in preparation of antitumor drugs.

At present, main fragrance products in the market comprise three types of fragrance candles, liquid fragrance and traditional burning incense. In the prior art, fire accidents are easy to occur, negative risks of PM2.5 to respiratory tracts of human bodies exist, articles are greatly wasted, degradation is not easy, fragile container products have potential harm to human and animals, and resources are wasted. The acceptability of people is lower than that of natural products. The inventor finds that the extracted remains and residues of plants have a large amount of aromatic substances for a long time, and the remains and residues of the plants are often directly discarded in the industry and are not wasted. Through six years of exploration, pure fragrance of a single plant without an adhesive acts on people, so that the people are benefited. And the product has the functions of a natural fragrance diffuser and an art ornament, and can be naturally degraded. The problem of treatment of plant residues and remains in an extraction factory is solved, and the plant residues and remains are effectively converted into high-added-value products.

The invention belongs to the technical field of natural products, and provides application of fucoxanthin in preparation of oral anti-alopecia drugs. Animal experiments find that long-term high-fat diet can lead to large-piece shedding of hair accompanied by skin inflammation, and fucoxanthin has a treatment effect on high-fat diet induced mouse back hair shedding and skin inflammation, can obviously relieve high-fat diet induced subcutaneous cholesterol crystal accumulation and sebaceous gland cell hypertrophy, and has the effects of preventing and treating the high-fat diet induced mouse back hair shedding and skin inflammation and preventing and treating the high-fat diet induced mouse back hair shedding and the high-fat diet induced sebaceous gland cell hypertrophy. And skin epidermal hyperplasia and cuticle thickening induced by high fat diet can be improved. Besides, cell experiments also find that fucoxanthin intervention can reduce oleic acid infiltration of the hair follicle stem cells and reduce oleic acid induced death and apoptosis of the hair follicle stem cells, and prove that fucoxanthin has a protection effect on the hair follicle stem cells, has low toxic and side effects on cells and animals as a natural algae extract, and has a good application prospect. Good clinical application prospects are realized.

The invention discloses mulberry leaf flavone as well as a preparation method and application thereof, and belongs to the technical field of natural product extraction and preparation. Flavone is released through a microwave-assisted enzyme method, flavone is dissolved through a deep eutectic solvent, the reaction rate is increased through microwave treatment, meanwhile, the catalytic activity of enzyme can be enhanced, and the enzymatic reaction speed and yield are effectively increased; the deep-eutectic solvent contains a large amount of hydroxyl groups and amino groups, the hydroxyl groups and the amino groups can easily form hydrogen bonds with flavone, the solubility of the flavone in the deep-eutectic solvent can be increased, and meanwhile the total extraction amount of the flavone is increased under the dual auxiliary effects of microwaves and enzyme; by adopting a resin separation method, high-purity mulberry leaf flavone, mulberry leaf alkaloid and mulberry leaf polysaccharide can be obtained. The mulberry leaf flavone and the mulberry leaf alkaloid which are prepared and separated by the method can be conveniently and rapidly proportioned according to the requirements, and the mulberry leaf flavone and the mulberry leaf alkaloid can be widely applied to the aspect of hypoglycemic food.

The invention discloses a high-efficiency green extraction method of semen plantaginis chitin, and belongs to the technical field of natural product extraction. The method comprises the steps of raw material pretreatment, ultrasonic-assisted enzymolysis, enzyme deactivation, centrifugal separation, ultrafiltration concentration, alcohol precipitation and drying. The core innovation lies in that an ultrasonic-assisted composite enzymolysis synergistic technology is adopted, the dissolution rate of the polysaccharide is remarkably increased, the enzyme activity is protected, and finally the high-purity polysaccharide with the molecular weight of 9.2 kDa and the extraction rate of 13.82% is obtained. Compared with a traditional method, intermittent ultrasound and low-temperature enzymolysis are cooperated, so that polysaccharide chain breakage is effectively reduced; the compound enzyme degrades cell wall components in a targeted manner and breaks through the efficiency bottleneck of a single enzyme; toxic solvents are avoided in the whole process, and the process is environment-friendly. The data of the embodiment shows that the extraction rate is increased by 30% or above compared with that of a single enzyme method, the molecular weight distribution of the product is uniform, and the method is suitable for large-scale production of functional polysaccharides in the fields of food, medicine and the like.