1843 results about "Natural product" patented technology

The invention relates to the field of natural products, in particular to a dimer compound of guaiane type sesquiterpenes and uric acid as well as an extraction and separation method and application of the dimer compound. According to the invention, a new dimer compound of guaiane type sesquiterpene and uric acid is extracted and separated from carpesium abrotanoides in Guizhou, and it is found that the dimer compound has relatively strong anti-tumor activity. The compound has strong inhibitory activity on breast cancer, prostate cancer, nasopharynx cancer, liver cancer, lung cancer, leukemia, pancreatic cancer, glioma, osteosarcoma, skin cancer, cervical cancer, ovarian cancer, kidney cancer and esophageal cancer, and provides an optional scheme for broad-spectrum anti-cancer drugs. Moreover, in human prostate cancer cells PC3, human cervical cancer cells Hela, human liver cancer cells HepG2 and human glioma cells U87 which are resistant to paclitaxel, the compound also shows strong anti-cancer activity, which indicates that the compound has the potential of treating patients resistant to paclitaxel.

The invention discloses a probiotic-polyphenol nanoparticle colon-targeted co-delivery system as well as a preparation method and application thereof, and belongs to the field of biological medicines. The FCPs and hyaluronic acid (HA) are combined through non-covalent interaction, so that the hydrogel has colon targeting property and mucosal adhesion at the same time, and delivery of probiotics is facilitated. The hydrogel (HF) based on polysaccharide has good biocompatibility, and can be used for effectively encapsulating the probiotics and the natural products. Then, the PDA-TH NPs and BA are incorporated into the polysaccharide chain network of HF, and finally the BA-HF-PDAT targeted co-delivery system is constructed. The BA (at) HF-PDAT targeted co-delivery system can protect BA from serious gastrointestinal stress, and is jointly assembled with PDT-TH NPs to realize dual slow release of thymol (Thy), so that the treatment effect of BA and Thy is improved, and the BA (at) HF-PDAT targeted co-delivery system contributes to treatment of clostridium difficile infection (CDI).

The invention provides homogenized pitaya polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of polysaccharide extraction and purification. The homogenized pitaya polysaccharide is a polysaccharide of which the monosaccharide is composed of mannose, glucosamine hydrochloride, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose, the relative molecular weight is 8.08 * 10 < 4 > Da, and the corresponding molar ratio is (0.181): (0.250): (16.544): (12.762): (3.793): (0.739): (51.014): (14.716). The invention further provides a preparation method of the homogenized pitaya polysaccharide and application of the homogenized pitaya polysaccharide in preparation of lipid-lowering and sugar-lowering drugs. The homogenized pitaya polysaccharide disclosed by the invention has an obvious lipid-lowering and blood-glucose-reducing effect, is from a natural product, so that the homogenized pitaya polysaccharide has no toxic or side effect and can be used for preparing lipid-lowering and blood-glucose-reducing medicines.

The invention relates to a preparation method and application of sea buckthorn pomace flavone extract freeze-dried powder, and belongs to the technical field of natural product extraction. The method comprises the following steps: pretreating (drying and powdering) a sea buckthorn pomace raw material, preparing a crude extract, purifying the extract, freeze-drying the extract, and finally obtaining the sea buckthorn pomace flavone extract freeze-dried powder. The by-product sea buckthorn pomace in the sea buckthorn processing process is subjected to resource utilization, the vibration type ultrafine pulverizer is adopted for pulverizing to increase the extraction surface area, the ultrasonic synergistic biological enzymolysis method is adopted for promoting release of active molecules, the efficiency is improved, energy and time are saved, no harmful solvent is used in the extraction process, and the method is environmentally friendly, simple in preparation process and high in operability; the large-scale production is easy to realize. According to the sea buckthorn pomace flavone extract freeze-dried powder prepared by the method, the total flavone extraction amount reaches 17.79-23.05 mg / g of the dry weight of sea buckthorn pomace, the purity can reach 50-65%, and the sea buckthorn pomace flavone extract freeze-dried powder has good oxidation resistance, alpha-glucosidase inhibitory activity and alpha-amylase inhibitory activity and can be used for developing products for reducing blood sugar.

The invention discloses a compound with anti-tumor activity. The compound has a chemical structural formula as shown in a formula (I). The compound belongs to a novel tetracyclic triterpenoid natural product, has good fat solubility and chemical stability, and can be conveniently extracted from the rhizome of a herbal medicine Polygala fallax Hemsl. And prepared into an oral or injection form. The invention also provides a preparation method and application of the compound. The compound provided by the invention can obviously inhibit the growth of mouse lung cancer and reduce the volume and weight of tumor, and can be used for treating tumor diseases.

The invention discloses a preparation method of a zinc-dihydromyricetin nano-enzyme and application of the zinc-dihydromyricetin nano-enzyme in preparation of drugs for promoting healing of diabetic wounds, and the zinc-dihydromyricetin metal polyphenol nano-enzyme (Zn-DHM) is obtained by coordinating a metal element zinc (Zn) and a natural product dihydromyricetin (DHM) through a one-step method by taking PVP as a surfactant. The Zn-DHM nano-enzyme prepared by the invention has antioxidant property and cell proliferation and migration promoting ability, and can maintain metabolism and immune homeostasis of diabetic wounds, so that the Zn-DHM nano-enzyme can accelerate early healing of the diabetic wounds.

The invention belongs to the field of natural product extraction, and particularly relates to a method for extracting chitosan by using hermetia illucens in a high-valued manner. According to the method, the ionic liquid is introduced while the chitosan is extracted by adopting a deep eutectic solvent method, the deep eutectic solvent can form strong interaction with chitin molecules, the ionic liquid can further enhance specific recognition of the chitin molecules, and the ionic liquid and the deep eutectic solvent have a synergistic effect, so that solubility and recognizability of the chitin are improved; protein and salt are efficiently removed, meanwhile, glucosidic bonds of a chitin macromolecular main chain are prevented from being hydrolyzed and broken, and the extraction rate and purity of chitosan are remarkably improved. According to the method, the extraction condition is mild, the eutectic solvent can be recycled and reused, the extraction process flow of the chitosan is simplified, and the method is environment-friendly.

The invention provides a method for synchronously extracting tea seed oil and tea saponin from tea seeds, and belongs to the technical field of active substance extraction. According to the method, the extraction rate and the purity are remarkably improved by finely regulating and controlling the extraction conditions. The specific composite oil extraction auxiliary agent and the ultrasonic-assisted extraction technology are adopted, and the power, time and solvent ratio are optimized, so that the extraction efficiency and the product quality of the tea seed oil and the tea saponin are superior to those of the tea seed oil and the tea saponin prepared by a traditional method. Experimental data prove that the extraction rate of the tea seed oil reaches 27.22%, and the purity of the tea seed oil reaches 99.66%; the extraction rate of the tea saponin is 21.85%, and the purity of the tea saponin reaches 96.50%. By increasing the utilization rate of the raw materials and reducing the production cost, the market competitiveness of the product is improved, the high-quality tea seed oil and tea saponin meet the requirements of the market for natural products, and the tea seed oil and tea saponin have wide application prospects.

The invention discloses a natural product-based nano antioxidant for treating acute pancreatitis and a preparation method of the natural product-based nano antioxidant, and relates to the technical field of biological medicines. 25 mg of ferulic acid (FA); and 15 mL of 80% (v / v) ethanol-deionized water solution. According to the natural product-based nano antioxidant for treating acute pancreatitis and the preparation method thereof, the nano antioxidant effectively targets inflammatory pancreatic tissues through the specific interaction of chondroitin sulfate (CS) and CD44, and is split and released under the stimulation of pH / GSH and H2O2 in cells to generate FA and zein degradation products, the FA and zein degradation products efficiently play a synergistic role in removing ROS, and the natural product-based nano antioxidant can be used for treating acute pancreatitis. Therefore, inflammation is relieved; the nano antioxidant shows an excellent in-vivo treatment effect on acute pancreatitis and has a clinical application prospect.

The invention discloses a method for extracting and purifying flavone in litsea coreana through an ultrasonic-assisted eutectic solvent, and belongs to the technical field of green processing of natural products. The invention provides a method for efficiently preparing litsea coreana flavone by integrating computer prediction, green solvent extraction and resin purification technologies. The method comprises the following steps: firstly, calculating and screening a deep eutectic solvent system with the highest affinity with the litsea coreana flavone on the basis of a COSMO-RS theoretical model, then coupling the optimized DES with an ultrasonic-assisted extraction (UAE) technology, and through the synergistic effect of the DES and the UAE technology, remarkably improving the extraction rate of the litsea coreana flavone and furthest keeping the biological activity of a target component; then, a macroporous resin dynamic adsorption-desorption purification process is linked, so that high-selectivity enrichment and impurity removal of the flavonoid compounds in the crude extract are realized, a high-purity product is obtained, and a complete process route from intelligent screening, efficient extraction to precise purification is finally formed.

The invention discloses a microcystis fungus, an anti-inflammatory and anti-osteoporosis compound as well as a preparation method and application of the microcystis fungus and the anti-inflammatory and anti-osteoporosis compound. According to the invention, two cyclic ester peptide compounds, i.e., Microascusin A (formula I) and Microascusin B (formula II), with new structures, are obtained from sponge-derived fungus Microascusin, i.e., Microascusin A (formula I) and Microascusin B (formula II), and the microascusin A (formula I) and Microascusin B (formula II) are obtained from sponge-derived fungus Microascusin. The microcystin A and the microcystin B are natural products obtained through microbial fermentation, the raw materials are easy to obtain, and the microcystin A and / or the microcystin B and the pharmaceutical composition can be used for preventing and treating inflammation and / or osteoporosis. The microcystin A and / or microcystin B can also be combined with known medicines to form a compound preparation for preventing and treating inflammation and / or osteoporosis. # imgabs0 #

The invention discloses a combined module for improving the yield of actinomycetes polyketone natural products and construction and application of recombinant bacteria containing the combined module, and belongs to the technical field of genetic engineering. In order to improve the yield of natural products such as polyketone, non-ribosome peptide and the like, the invention provides a combined module capable of improving the yield of actinomycetes natural products by combining and transforming a synthesis pathway of CoASH and a post-modification pathway of PPTase through a metabolic engineering strategy, and the module consists of CoASH cofactor synthesis module elements SCoaAR106A and SCoaD and a PPTase post-modification module element HPC3. By constructing a recombinant vector and recombinant bacteria containing the combined module, the yield of natural products such as actinomycetes polyketone, polyether and non-ribosomal peptide can be effectively improved by over-expression of the combined module.

Methods to generate novel acyl-tRNA species deploy an orthogonal synthetase that accepts α-hydroxy acids, α-thio acids, N-formyl-L-α-amino acids, and / or α-carboxyl acid monomers (malonic acids) that are formally precursors to polyketide natural products.

The invention discloses application of a microbial natural product GameXPeptide-A in improving the pest control effect of an insecticide, and belongs to the technical field of biological control. Experiments prove that after the GameXPeptide-A is combined with insecticides such as metarhizium anisopliae, beauveria bassiana, nuclear polyhedrosis virus, 4% of abamectin acetamiprid, 10% of spinosad emamectin benzoate, 30% of etoxazole nitrile, 45% of biphenyl etoxazole, 5% of emamectin benzoate, 5% of chlorantraniliprole, 5% of abamectin and the like for use, the insecticidal effect is obviously improved; the insecticidal activity of the insecticide on agricultural pests such as thrips, ostrinia nubilalis and prodenia litura can be effectively improved, so that the substance can be combined with different insecticides for use, and the insecticidal activity of the insecticide on crop pests in agricultural production such as lepidoptera, diptera, coleoptera, orthoptera, hemiptera and homoptera is improved; the compound can be used for development and application of novel pest immunosuppressants or insecticide synergists.

The invention belongs to the field of natural products, and discloses camellia oleifera shell polysaccharide as well as a preparation method and application thereof. The structural formula of the camellia oleifera shell polysaccharide is # imgabs0. The camellia oleifera shell polysaccharide of some examples is low in molecular weight, better in thermal stability, good in anti-inflammatory activity and free of toxicity to cells.

The invention discloses an osthole compound, a metal complex and a preparation method and application of the osthole compound, the osthole compound (OST-Alkyne) has remarkable anti-tumor biological activity, osthole (OST) and a cyclometalated iridium precursor are further connected through a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction, higher anti-tumor activity is shown, and the osthole compound has a good application prospect. Mitochondria is targeted, and tumor is killed by reducing mitochondrial membrane potential and inducing cell autophagy and necrotic apoptosis; according to the invention, natural products are combined with metals, so that not only are good selectivity and anti-proliferation ability to brain glioma cells realized, but also toxic and side effects to normal cells are overcome, a new design idea is provided for novel metal drugs to treat brain glioma, and the application prospect is wide.

The present invention provides a cornus officinalis fruit extract rich in morroniside and a preparation method and application thereof, and relates to the technical field of plant natural products. The cornus officinalis fruit extract rich in morroniside comprises morroniside, strychnoside and swertiaside, wherein the content of morroniside is 10% to 12%. The cornus officinalis fruit extract rich in morroniside of the present invention has excellent anti-inflammatory effect, can promote skin firmness and reduce wrinkles, and can be widely used in the preparation of cosmetics and skin care products. The preparation method of the cornus officinalis fruit extract rich in morroniside of the present invention efficiently extracts the active components in the cornus officinalis fruit by ethanol extraction and enzymatic hydrolysis treatment, and obtains the cornus officinalis fruit extract enriched with morroniside by combining column chromatography. The method is simple in process and has strong operability.

The invention provides a screening platform for chemotherapy combined anti-tumor natural products and application of the screening platform. PTC has tumor cells, interstitial cells, fibroblasts and immune cells from primary epithelium, is rich in cell components, and has advantages in the aspect of tumor microenvironment simulation. Meanwhile, PTC can reproduce tumor heterogeneity, the culture success rate and clinical consistency can reach 90% or above, and the detection period is within two weeks. And the detection result can be read through AI intelligent software, so that the accuracy can be further improved. The screening period can be shortened, the cost is reduced, and the accuracy is improved; according to the platform, high-throughput screening can be carried out in a short time, and the treatment effect of a chemotherapy drug combined anti-tumor natural product drug scheme can be predicted.

The invention relates to the technical field of plant effective component extraction, in particular to a deep-eutectic solvent and application thereof, and the deep-eutectic solvent is composed of anhydrous betaine, DL-malic acid and deionized water. The eutecticevaporate solvent is applied to extraction of momordica grosvenori polysaccharide, ultrasonic waves are combined with the eutecticevaporate solvent, in addition, extraction conditions of crude momordica grosvenori polysaccharide are optimized, and a new method is provided for efficient and green extraction of momordica grosvenori polysaccharide. The new method not only expands the application range of DESs in natural product extraction, but also provides important technical reference for industrial development of momordica grosvenori polysaccharide.

The invention discloses pancreatic lipase immobilized composite nano magnetic beads, a preparation method and application, ferroferric oxide is adopted as a magnetic core, dopamine-mediated growth is performed to form a core-shell structure, then the periphery of the core-shell structure is wrapped with a metal organic framework to form the composite nano magnetic beads, and pancreatic lipase is immobilized by using the composite nano magnetic beads as an adsorption carrier. The enzyme loading amount, the repeated utilization rate and the storage stability of the ferroferric oxide nano-particles are remarkably improved, the enzyme immobilization efficiency and the enzyme activity are improved, and meanwhile, the prepared pancreatic lipase immobilized composite nano-magnetic beads have excellent stability and biocompatibility, are green and pollution-free and can be repeatedly used; the composite nano magnetic beads are synthesized by adopting a solvothermal method, the product is easy to separate, the synthesis process is simple, the enzyme immobilization efficiency can be regulated and controlled by regulating and controlling the synthesis process, the structure of an active ligand can be screened and analyzed from natural products with numerous and jumbled components, and the active ligand has a certain lipid-lowering potential and can be used for preparing the nano magnetic beads. The compound can be used as a potential lead compound of lipid-lowering drugs.

The invention belongs to the field of natural product extraction, and relates to a method for extracting and separating flavonoid compounds from Artemisia chrysantha, which comprises the following steps: carrying out reflux extraction on the dry overground part of Artemisia chrysantha by using 70% ethanol, loading to a balanced D101 macroporous adsorption resin column, sequentially eluting by using water, a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution, and collecting the eluent. Four fractions from Fr. I to Fr. IV are obtained; respectively carrying out polyamide column chromatography separation on Fr.II to Fr.IV, and sequentially eluting with a 20% ethanol aqueous solution, a 50% ethanol aqueous solution and a 70% ethanol aqueous solution to respectively obtain three fractions; and further purifying the Fr.II-1, the Fr.II-2, the Fr.III-1, the Fr.III-2, the Fr.III-1 and the Fr.III-3 by virtue of a Sephadex LH-20 column, so as to finally obtain seven flavonoid compounds. According to the method, only the ethanol water low-toxicity solvent is used for extraction and preparation, organic solvents with high toxicity are not used, the method is green and environment-friendly, the extraction efficiency is high, the number of extracted compounds is large, chemical components of the artemisia europaea medicinal material are enriched, the resource source of active components is widened, and a foundation is laid for follow-up research.

The invention discloses a method for extracting total flavonoids from Chinese redbud seeds, and belongs to the technical field of natural product extraction and separation. The method adopts a deep eutectic solvent (DES) as an extraction medium, and specifically comprises the following steps: mixing dried Chinese redbud seed powder with the DES, carrying out ultrasonic-assisted extraction, and then carrying out centrifugal separation; and purifying the extracting solution by using macroporous adsorption resin, and eluting by using distilled water and ethanol in sequence to finally obtain the total flavonoids. According to the invention, cyclic utilization of the DES solvent is innovatively realized, DES in the loading effluent and the distilled water eluent is recovered through evaporation, and the DES solvent can be repeatedly used for at least three times. Compared with a traditional ethanol reflux extraction method, the method has the remarkable advantages that the total flavone extraction amount is increased by 3 times, and the method is simple and convenient in operation process, high in safety, environment-friendly and low in cost. The method not only improves the extraction efficiency, but also meets the requirements of modern green processing, and provides a new technical approach for sustainable development of natural products.

The invention discloses a plant extraction and purification method of glycyrrhizic coumarin, and belongs to the technical field of natural product extraction. The method comprises a purification method suitable for industrial production of glycyrrhizic coumarin and a purification method for laboratory preparation of glycyrrhizic coumarin. According to the laboratory purification method, licorice extract sequentially passes through a silica gel column and a C18 column for coarse separation and then is refined through semi-preparative liquid chromatography, the glycyrrhizic coumarin with the purity higher than 98% is obtained and can be used as a standard substance for licorice quality research, and an important basis is provided for further deep research and development of glycyrrhizic coumarin. According to the present invention, the industrial production method is based on the laboratory extraction step, the steps are simplified, such that the method is suitable for the pilot scale production, the steps are simple, the efficiency is high, the yield is high, the glycyrrhizic coumarin with the purity of more than 98% can be obtained through the separation, and the advantages are provided for the further research and the wide application of the glycyrrhizic coumarin.

To provide a new composition consisting of 100% natural matter, exerting a therapeutic or beneficial effect in the treatment of bone fragility, having a physiological mode of action.SOLUTION: The present invention provides a 100% natural product consisting of natural matrices exhibiting emerging properties, which enables re-establishing correct bone metabolism by correcting behavior of mesenchymal stem cells and re-establishing a correct balance between cell populations that allow bone growth or remodeling. The natural product consists of, for example, Equisetum flowering tops, Acacia, Malpighia fruit, coral skeleton, avian eggshell, Agave leaves, Cetraria stems, Agaricus, and calcium citrate.SELECTED DRAWING: None

The invention belongs to the field of microbial natural product pesticides, and particularly discloses a polyketone compound as well as a preparation method and application thereof. The compound disclosed by the invention is prepared from a fermentation crude extract of a strain of tobacco endophytic fungus Aspergillus japonicus TE-739D, and the compound disclosed by the invention is prepared from the fermentation crude extract of the tobacco endophytic fungus Aspergillus japonicus TE-739D. The chemical structure of the compound is identified through a modern spectroscopy technology, such as a high resolution mass spectrum HRESIMS and a superconducting nuclear magnetic resonance spectrum NMR, and the compound is named as japonidiene C. The invention further discloses a preparation method of the compound. The polyketone compound japondiene C has an obvious insecticidal activity on 2-instar larvae of spodoptera frugiperda, and can be used for preventing and treating the second instar larvae of the spodoptera frugiperda.

The invention discloses a method for extracting eucommia ulmoides with an intestinal repair function, and relates to the technical field of natural product extraction.The method comprises the steps that a fresh eucommia ulmoides raw material is subjected to low-temperature drying and smashing, a polysaccharide water extract and filter residues are obtained through water extraction, and then the flavone residual quantity in the polysaccharide water extract is synchronously detected; carrying out enzymolysis on the polysaccharide water extract through beta-glucanase to obtain a small molecular polysaccharide solution, and carrying out alcohol extraction on filter residues to obtain a flavone alcohol extract; the preparation method comprises the following steps: mixing the two components according to a ratio, inoculating compound probiotics for anaerobic fermentation, regulating according to the residual quantity of flavone, regulating an alcohol extraction process by using short-chain fatty acid generated by fermentation when the residual quantity is high, supplementing flavone extract in an enzymolysis system when the residual quantity is low, and finally removing impurities, purifying, adapting intestinal tracts and drying to obtain the product. According to the method, regulation and control operation is correspondingly executed by detecting the residual quantity of flavone in the polysaccharide water extract, so that self-supply of a regulation and control reagent and high efficiency of active ingredients are realized, the conversion rate of small molecular polysaccharide and flavone aglycone is increased, and the intestinal tract tolerance of the product is optimized.

The invention discloses a limonin compound and application thereof in preparation of a medicine for preventing and / or treating cancers. The structure of the limonin compound is shown as a formula I or II. The cancer is at least one of breast cancer, liver cancer, gastric cancer, esophageal cancer, leukemia, pancreatic cancer, glioma, osteosarcoma, prostatic cancer, nasopharynx cancer, cervical cancer, ovarian cancer, kidney cancer and skin cancer. According to the invention, activity screening is carried out on various natural product extracts, it is found for the first time that limonin compounds with structural formulas I and II have excellent inhibitory activity on various different types of human cancer cells, and an optional scheme is provided for broad-spectrum anti-cancer drugs. Related results also show that the compound provided by the invention can inhibit migration and invasion ability of breast cancer cells, promote cell apoptosis and retard a cell cycle. The limonin compound applied in the invention is derived from mallotus oblongifolius, the source is wide, and the production cost is low.

The invention provides a method for extracting polyphenol from persimmon peel and application, the method comprises the steps of persimmon peel pretreatment, irradiation treatment, subcritical ethanol extraction and extraction of polyphenol in different forms, and the method belongs to the technical field of natural product extraction methods. According to the method, electron beam irradiation is utilized to treat persimmon peels, high energy in electron beams can bombard components such as cellulose and lignin in cell walls of the persimmon peels, tightly-combined chemical bonds are broken, ethanol in a subcritical extraction technology can permeate into the persimmon peels to extract polyphenol substances, and therefore the extraction efficiency of polyphenol is improved; and the consumption of the solvent in the extraction process can be reduced. Different forms of polyphenols are further extracted on the basis of the persimmon peel polyphenol crude extract, the polyphenol has good antioxidant capacity and alpha-glucosidase inhibitory activity, bioactive compounds in persimmon peel can be efficiently recycled, and future waste utilization and development of natural antioxidants are promoted.

The invention discloses a liver protection gastrodia elata polyphenol extract as well as a preparation method and application thereof, and belongs to the technical field of natural product extraction. The method aims at solving the problems that in the prior art, gastrodia elata polyphenol extraction efficiency is low, and active ingredients are prone to being damaged by heat. According to the technical scheme, the method is characterized by comprising the following steps: mixing a gastrodia elata raw material with liquid nitrogen after being subjected to freezing and unfreezing circulating pretreatment, and performing cell wall breaking treatment through a high-voltage pulsed electric field; mixing the wall-broken raw material with a buffer solution, carrying out enzymolysis by virtue of an immobilized enzyme reactor, cooling the system after enzymolysis, and carrying out ultrahigh-pressure extraction; centrifugally filtering the extracting solution, purifying through a macroporous adsorption resin column, and collecting ethanol eluent; and finally, carrying out vacuum concentration and spray drying on the eluent to obtain the gastrodia elata polyphenol extract. According to the method, the polyphenol yield is effectively improved, the activity of the components is protected, and the obtained extract can be used for preparing liver protection medicines.

Disclosed are modified Type 1 O-methyltransferases (OMTs) and methods of using such modified OMTs. In some forms of the modified OMTs, the amino acid corresponding to Phe337 of Sorghum bicolor stilbene OMT (SbSOMT) is substituted with a polar amino acid. In some forms of the modified OMTs, the amino acid corresponding to Asn323 of Sorghum bicolor caffeic acid OMT (SbCOMT) is substituted with a hydrophobic amino acid. In some forms of the modified OMTs, the amino acid corresponding to Ile144 of SbSOMT is substituted with a polar amino acid. In some forms of the modified OMTs, the amino acid corresponding to Asn128 of SbCOMT is substituted with a hydrophobic amino acid.