6 results about "Equisetum" patented technology

The invention discloses mucor oosperm and application of the mucor oosperm, the bacterial strain number of the mucor oosperm is JSAFC 2483, the mucor oosperm is preserved in the China General Microbiological Culture Collection Center on May 6, 2025, and the preservation number is CGMCC No.41980. The mucor oosperm has the advantages that the mucor oosperm can be used for preparing the mucor oosperm; the biocontrol bacterium Mucor moelleni JSAFC 2483 provided by the invention can effectively inhibit the growth of Fusarium equiseti, the inhibition rate of plate confrontation culture reaches 54.90%, meanwhile, extracellular metabolites secreted by the biocontrol bacterium Mucor moelleni JSAFC 2483 can also significantly reduce the growth of the Fusarium equiseti, when the concentration of the extracellular metabolites of the JSAFC 2483 is 10 mL / 100 mL, the inhibition rate of the Fusarium equiseti reaches 53.98%, and when the concentration of the extracellular metabolites of the JSAFC 2483 is 100 mL / 100 mL, the inhibition rate of the Fusarium equiseti reaches 56.64%. Meanwhile, the plant growth promoting effect is achieved on pepper plants.

The invention relates to a'kit and a detection method for detecting fusarium equiseti based on an RPA-CRISPR / Cas system ', and belongs to a molecular detection technology, and the RPA-CRISPR / Cas system adopts an RPA primer pair and an sgRNA sequence of the following sequence information: FequF: 5'-TAATCGTGAGATCACTAATAGCAAAGTAC-3 '; according to the present invention, with the application of the Fusarium equiseti gene in the field, the sample suspected to be infected with the Fusarium equiseti can be rapidly detected on the field, the detection sensitivity can achieve 79.5 * 10 <-6 > ng / [mu] L, and the important significance is provided for early detection of the infection of the corn stem rot pathogenic bacteria, prevention of the spread of the pathogenic bacteria infection, and accurate adoption of the plant protection measure to reduce the corn yield loss. The method can be used for rapidly detecting the sample suspected to be infected with the Fusarium equiseti gene in the field, and can be used for rapidly detecting the sample suspected to be infected with the Fusarium equiseti gene in the field, and can be used for rapidly detecting the sample suspected to be infected with the Fusarium equiseti gene in the field.

The invention belongs to the technical field of analytical chemistry, and particularly relates to a method for simultaneously determining 13 chemical components in horsetail based on an HPLC-MS / MS (High Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) method. The invention provides a method for simultaneously determining 13 chemical components in horsetail based on an HPLC-MS / MS (High Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) method. The method is good in linear relationship (the 13 chemical components in the horsetail medicinal material are good in linear relationship (r gt; the method has the advantages of high accuracy, high repeatability and high recovery rate (the average sample recovery rate is 86.67-110.91%), can simultaneously and accurately determine the content of 13 chemical components in the equisetum hyemale medicinal material, and meets the requirement of comprehensively evaluating the quality of the equisetum hyemale.

The present invention belongs to the field of biological control, and particularly relates to a biocontrol fungus capable of infecting and controlling solanaceous plants and an optimization method for its fermentation and culture conditions. The present invention discloses a method for preventing bacteria in solanaceous plants and optimizing its fermentation and culture conditions. Among them, the biocontrol bacteria are Alternaria alternata, Epichloë sorghi, Fusarium equiseti, Curvularia hawaiiensis and / or Nigrospora oryzae. The present invention obtains pathogenic fungi with strong pathogenicity to solanaceous plants by collecting naturally diseased plants, isolating, culturing and purifying them indoors, and verifying the pathogenic bacteria by Koch's postulates.

This invention discloses a *Equisetum hyemale* polysaccharide, its preparation method, and its applications, belonging to the field of natural polysaccharide technology. Using *Equisetum hyemale* as raw material, the polysaccharide was extracted using a hot water ultrasonic-assisted extraction method. Through single-factor and orthogonal experiments, the optimal process was determined to be a material-to-liquid ratio of 1:20 g / mL, a temperature of 70℃, an ultrasonic power of 400 W, and an extraction time of 50 min. Under these conditions, the polysaccharide yield reached 13.05%. The crude polysaccharide was deproteinized using the Sevage method and purified by DEAE-52 cellulose column and Sephadex G-100 gel column to obtain purified *Equisetum hyemale* polysaccharide (DEPS) with homogeneous components. Physicochemical characterization showed that the DEPS had a total sugar content of 55.36%, a molecular weight of 67.61 kDa, and was composed of mannose, glucuronic acid, glucose, and fucose. It lacked a triple helix structure and contained typical polysaccharide functional groups and pyranose ring structures. In vitro experiments showed that DEPS exhibited the highest scavenging rates of DPPH, ABTS, and hydroxyl radicals, reaching 87.54%, 97.52%, and 97.5%, respectively, with its antioxidant activity showing a concentration-dependent effect. This invention features a simple process and high yield, producing a high-quality Equisetum hyemale polysaccharide.

The invention provides fusarium equiseti RT199, a fermentation inoculant and application of the fusarium equiseti RT199, and belongs to the technical field of microorganisms. The invention provides fusarium equiseti RT199, and the preservation number of the fusarium equiseti RT199 is CGMCC (China General Microbiological Culture Collection Center) No.41794. The fusarium equiseti RT199 has the capability of secreting cellulase, laccase and xylanase; the fusarium equiseti RT199 has the effects of efficiently degrading straws and improving soil. The result of the embodiment shows that the degradation rate of the fusarium equisetum RT199 on the corn straw at the normal temperature of 25-30 DEG C reaches 70.53%; the fusarium equiseti RT199 is inoculated to straw, then the straw inoculated with the inoculant is returned to the field, and after the straw is returned to the field for 30 days, compared with a control group, the POC content of soil is increased by 19.16%, MAOC is increased by 39.59%, and the total organic carbon content of the soil is increased by 31.99%.