The invention discloses a vector for recombinant expression of panda follicle-stimulating hormone, an expression system and a preparation method thereof, which relate to the technical field of gene engineering. The vector comprises a first gene for encoding a panda FSH alpha subunit and a second gene for encoding a panda FSH beta subunit, the sequence of the first gene is shown as SEQ ID NO.1, andthe sequence of the second gene is shown as SEQ ID NO.2. By constructing the vector and the expression system for recombinant expression of the panda follicle-stimulating hormone, stable and continuous active expression of the panda FSH alpha subunit, the FSH beta subunit and subunit compounds thereof can be realized. By using the panda-derived follicle-stimulating hormone, the risk of adverse reaction of organisms is reduced, and the breeding and propagation of varieties of rare endangered felines in China, such as giant pandas, small pandas and the like, are facilitated. In addition, the recombinant preparation method is simple and low in cost.