Living body cold storage method of serangium japonicum

A kind of technology of ladybug and living body, which is applied in the field of cold storage of live ladybug, can solve the problem that Japanese ladybug cannot be stored, and achieve the effect of improving control efficiency, convenient storage and deepening understanding

Active Publication Date: 2015-06-10
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, and to provide a method for refrigerating the living body of the Japanese ladybug, which realizes the diapause control of the Japanese ladybug by adjusting temperature and photoperiod, so that the Japanese ladybug Insects can be stored in ordinary refrigerators for a long time without supplementing prey, which solves the problem that live Japanese ladybugs cannot be stored

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0033] (1) Acquisition of insect source: Collect ladybug larvae from the eggplant field of Hunan Academy of Agricultural Sciences, and put them into the room for breeding. The feeding container is a disposable transparent plastic cup, sealed with medical gauze with a rubber band, and each feeding container A larva of Ladybug japonica was placed in the center, and the feed was eggplant leaves with living eggs, nymphs or pseudopupa of Bemisia tabaci, and the feed was changed every two days. The breeding environment is an ordinary indoor environment, the ambient temperature is 22-30°C, the humidity is 60%-80%, and the photoperiod is 14 hours in a light environment and 10 hours in a dark environment. After the larvae of the Japanese ladybug pupate, continue to cultivate for 4 days. At this time, the pupae of S. japonica will emerge within 6 hours or the adults of S. japonica just eclosion will not exceed 12 hours, and these nymphs or adults are used as the worm source of diapause ...

Embodiment 2

[0039] (1) Insect source acquisition: The larvae of Ladybug japonica were collected from the eggplant field of Hunan Academy of Agricultural Sciences, and placed in the room for rearing. The rearing container was a disposable transparent plastic cup, which was sealed with medical gauze with rubber bands. Each rearing container was sealed with medical gauze. A larvae of the Japanese lady beetle was placed in the middle, and the feed was eggplant leaves with live eggs, nymphs or pseudopupae of Bemisia tabaci, and the feed was changed every two days. The rearing environment was an ordinary indoor environment, the ambient temperature was 22-30°C, the humidity was 60%-80%, and the photoperiod was 13 hours in a light environment and 11 hours in a dark environment. After the larvae of the Japanese lady beetle pupated, the culture was continued for 4 days. At this time, the pupae of L. japonicus will emerge within 6 hours or the adults of L. japonicus will emerge within 6 hours or not...

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Abstract

The invention provides a living body cold storage method of serangium japonicum. The method includes steps of diapause induction, cold coma storage, diapause termination and the like. The method specifically includes that the serangium japonicum is put in an environment with alternate illumination and darkness for 240 hours so that the serangium japonicum enters diapause; the serangium japonicum that enters diapause is stored at low temperature in a dark place; the serangium japonicum that is stored at low temperature in the dark place is taken out and put at the room temperature under lighting conditions for 20 minutes, and the serangium japonicum starts to move and restores to eat; after 8 days in the environment of 22-28 DEG C and photoperiod (L: D= 13h: 11h), egg production capacity of female adults of the serangium japonicum is recovered. By means of the living body cold storage method of the serangium japonicum, diapause adjustment and control of the serangium japonicum can be achieved through adjustment of temperature and photoperiod, the serangium japonicum can be stored in a common refrigerator for a long time without supplement of preys, the problem of failure of storage of live serangium japonicum can be solved, and propagation expanding of the serangium japonicum can be facilitated whenever possible.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a method for refrigerating living bodies of the Japanese ladybug. Background technique [0002] The crop yield loss caused by agricultural diseases and insect pests accounts for 10-15% of the total loss every year in my country. The scientific management of agricultural diseases and insect pests has important economic and social benefits for the implementation of the national food security strategy and the promotion of high agricultural yields. Whitefly pests seriously harm various crops, including mulberry trees, tea trees, citrus, flowers, cotton, vegetables, etc. Among them, Bemisia tabaci, which harms vegetables, has no specific drugs to control it due to its strong drug resistance. Greenhouses often seriously damage vegetables for a long time, and are called "super pests". [0003] The use of natural enemy insects to control whiteflies can continuously control the damag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
CPCA01K67/033A01N1/0278
Inventor 胡雅辉魏林刘勇梁志怀陈浩涛
Owner HUNAN PLANT PROTECTION INST
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