50 results about "Mulberry trees" patented technology

The invention discloses a mulberry SNP (Single Nucleotide Polymorphism) marker mining and stress resistance character prediction method based on a Transform architecture, and belongs to the crossing field of biotechnology and artificial intelligence. According to the method, mulberry genome sequencing and stress resistance phenotype data are collected, high-credibility SNP is screened through quality filtering and Bayesian statistics, a Transform model containing multiple self-attention mechanisms is constructed after multi-dimensional features are extracted, early stop method training optimization is combined, stress resistance character prediction is achieved, and the key SNP contribution degree is analyzed through feature disturbance. According to the method, the problems of insufficient long-distance feature correlation capture, low prediction precision, poor model interpretability and the like in the traditional technology are effectively solved, the stress resistance character prediction precision and the SNP marker credibility are improved, and an interpretable efficient screening strategy is provided for mulberry molecular breeding.

The invention discloses an application of kaempferol in preparation of a medicine for treating or preventing PEDV (Porcine Epidemic Diarrhea Virus) infection. The kaempferol can obviously inhibit the replication of the PEDV in Vero and LLC-PK1 cells through 3C-like protease targeting the PEDV, so that the kaempferol can be used for preparing the medicine for preventing and treating the PEDV. Besides, kaempferol, as a natural substance separated from mulberries, can be used as a feed additive for a long time so as to achieve the purpose of preventing epidemic diseases, and does not cause generation of drug-resistant strains and other toxic and side effects after being used for a long time; the defects of immune failure possibly caused by vaccination, virus variation caused by use of antiviral drugs and the like are overcome.

The invention discloses a mulberry leaf picking and positioning method and device based on machine vision, and relates to the technical field of machine vision. The method comprises the following steps: acquiring calibrated and synchronized multi-modal image data of a mulberry target area, wherein the data can be a binocular sequence or a depth camera color-depth pair; performing preprocessing including brightness color normalization and image degradation compensation on the data to obtain an enhanced frame; inputting the enhanced frame into a target detection and maturity evaluation joint network, identifying each mulberry leaf instance, and outputting an instance segmentation mask defining the contour of the mulberry leaf instance and a probability vector representing the maturity; based on the instance segmentation mask and depth information, pixels in the mask are converted into a three-dimensional leaf surface point cloud; according to the point cloud, the six-degree-of-freedom pose of each mulberry leaf target is obtained, and at least one candidate picking site is determined; and finally, performing priority ranking on the candidate picking sites according to a preset comprehensive scoring function, and generating a scheduling queue containing timestamps, six-degree-of-freedom poses and priorities.

The utility model discloses a saline-alkali resistant mulberry root placing sleeve, which relates to the technical field of tree protection equipment, and comprises a protection box, a placing sleeve and a limiting piece, the interior of the protection box is hollow, the top surface of the protection box is provided with the limiting piece, the limiting piece is used for limiting a trunk main body, the top surface of the protection box is provided with a mounting opening, and the mounting opening is communicated with the placing sleeve. A placement sleeve is mounted in the mounting opening through a bolt, the placement sleeve is of a hollow semicircular structure as a whole, the bottom of the placement sleeve is located in the protection box, and a top opening and the mounting opening are located on the same horizontal plane; according to the mulberry tree protection device, the placement sleeve and the protection box are used in a matched mode, a tree root only needs to be placed in the placement sleeve, the bottom of the tree root abuts against the bottom face of the placement sleeve, a tree trunk is limited through the limiting piece, placement protection of the whole protection component and a mulberry tree can be completed, excessive bolt installation and tree root movement are not needed, and the labor intensity of workers is reduced. The tree roots can be protected to the maximum extent, and soil in the tree roots is protected.

The utility model relates to the technical field of agricultural equipment, and discloses a lime spreader for mulberry planting site marking, which comprises a lime spreading vehicle, the upper surface of the lime spreading vehicle is connected with a transverse adjusting ruler and a longitudinal adjusting ruler, and the two ends of the transverse adjusting ruler are connected with transverse extension rulers. One end, far away from the head of the lime spreading truck, of the longitudinal adjusting ruler is connected with a longitudinal extension ruler; and the longitudinal adjusting ruler is movably adjusted along the center line of the lime spreading truck. According to the lime scattering device for mulberry planting site marking, the transverse adjusting ruler and the transverse extending ruler are matched with the longitudinal adjusting ruler and the longitudinal extending ruler, the distances between the planting site and other planting sites around can be accurately and rapidly measured, and therefore it is guaranteed that the distance between each planting marking point meets the planting requirement; the accuracy of field planting position marking is improved, and the field planting marking efficiency is improved.

The utility model relates to the field of mulberry fertilization, and discloses a quantitative fertilization device for mulberry planting, which comprises two annular water tanks, one side of each annular water tank is provided with a fixing device, the bottom of each annular water tank is provided with a spring water pipe, and the bottom of each spring water pipe is provided with a fertilization mechanism. The top of the annular water tank is fixedly connected with a supporting rod, the top of the supporting rod is fixedly connected with a supporting plate, and the top of the supporting plate is fixedly connected with an electric telescopic rod. According to the fruit tree fertilizing device, through cooperative use of the annular water tank, the spring water pipe, the supporting rod, the electric telescopic rod, the piston block, the closed water tank, the fixing cone, the discharging opening, an anti-skid pad and other structures, quantitative fertilization can be remotely conducted on fruit trees, fertilization work does not need to be conducted on the fruit trees manually in sequence, the manual labor amount is reduced, and the fertilization efficiency is improved; the time consumed by fertilization is shortened, and the device is convenient to use.

The present application relates to a preparation method of high-efficiency stable mulberry root extract containing oxyresveratrol and its application in relieving skin itching, and belongs to the technical field of cosmetics. The preparation method of the mulberry root extract containing oxyresveratrol comprises the following steps: drying and crushing the root of a mulberry tree, adding the crushed root into an aqueous solution of a eutectic solvent to perform ultrasonic extraction, centrifuging the product to obtain the mulberry root extract containing oxyresveratrol. The present application is based on a green solvent system composed of betaine and 1,3-propanediol / ethyl lactate, and combines with mild ultrasonic conditions to realize the efficient extraction of oxyresveratrol from the mulberry root, so that the extraction rate is improved and the risk of high-temperature oxidation is avoided. Moreover, the obtained extract can not only long-acting inhibit the itch receptor MRGPX1, but also is suitable for sensitive skin due to the removal of irritating components, and has high efficiency and biocompatibility, thereby breaking through the environmental pollution and technical bottleneck of the traditional method and having a wide market application prospect.

The application provides a pruning device and a high-yield pruning method for long fruit mulberry trees, which comprises a handle shell, battery shells and transmission shells arranged at two ends of the handle shell respectively, a pruning head arranged at a free end of the transmission shell, a pruning disc rotatably connected in the pruning head, a plurality of hook-shaped pruning teeth arranged at the edge of the pruning disc in a circumferential distribution, a pruning inlet arranged at one side of the pruning head, two cutting blades arranged at one side of the pruning inlet and matched with the hook-shaped pruning teeth, a driving motor arranged in the handle shell and used for driving the pruning disc to rotate, a liquid storage groove arranged in the pruning head, a dyeing groove arranged at the bottom of the liquid storage groove and extending inwardly on both sides of the hook-shaped pruning teeth, a liquid outlet arranged at one side of each dyeing groove, a rotating shaft rotatably connected to each liquid outlet and capable of rotating with the pruning disc, and a water-absorbing dyeing liquid sleeve arranged outside each rotating shaft. The pruning device can automatically disinfect the cutting blades while pruning, improves the pruning efficiency and reduces the labor intensity.

The invention discloses application of a mulberry calcium transporter MmCAX and a coding gene thereof in manganese stress resistance of plants, the amino acid sequence of the mulberry calcium transporter MmCAX is shown as SEQ ID NO: 2, and the nucleotide sequence of the gene is shown as SEQ ID NO: 1. Through analysis of related physiological and biochemical indexes of mulberry trees under manganese stress and research on the mulberry tree calcium transporter MmCAX, the mulberry tree genome is perfected, and expression analysis and application of the mulberry tree calcium transporter under the manganese stress condition are provided. The mulberry calcium transporter gene MmCAX is transferred into agrobacterium tumefaciens GV3101 by using a VIGS silencing technology, and after gene expression is reduced by using a virus silencing principle, physiological and biochemical indexes of a transgenic plant are detected and the effect of the mulberry calcium transporter gene MmCAX in the aspect of improving the stress resistance of the plant is researched. An important molecular basis is provided for researching a mulberry manganese stress resistance regulation mechanism and cultivating a new variety.

The utility model discloses a shoot harvester for mulberry planting, which belongs to the technical field of mulberry planting and comprises a driving component, a connecting rod is sleeved at the output end of the driving component, an operating rod is sleeved outside the connecting rod, one side of the connecting rod is fixedly connected with a cutting blade, and the cutting blade is electrically connected with the driving component through a lead. A strap is fixedly connected to the outer portion of the connecting rod, and a shielding device used for blocking cutting chippings is installed at the position, close to the cutting blade, of the connecting rod. According to the mulberry branch cutting device, when a mulberry branch needs to be cut, the mounting plate and the fixing plate are arranged at the proper positions of the connecting rod in a sleeving mode, then the threaded handle is rotated, the threaded handle is clamped in the fixing plate, rotation continues to be conducted, the mounting plate and the fixing plate are fixed outside the connecting rod, and in the cutting process, chippings are thrown to the baffle through rotation of the cutting piece, so that the mulberry branch is cut. And therefore, the blocking of the scraps is realized, and the problem of injury to operators is avoided.

The invention discloses an application of mulberone H in preparation of a medicine for treating or preventing PEDV (Porcine Epidemic Diarrhea Virus) infection. In-vitro test results prove that the mulberone H significantly inhibits the enzyme digestion activity of PEDV 3C-like protease, and further verifies that the mulberone H inhibits PEDV infected cells, so that the mulberone H can be used for preparing the medicine for preventing and treating PEDV. Besides, mulberone H, as a natural substance separated from mulberries, can be used as a feed additive for a long time so as to achieve the purpose of preventing epidemic diseases, does not cause generation of drug-resistant strains after long-term use, and does not have other toxic or side effects. The defects of immune failure possibly caused by vaccination, virus variation caused by use of antiviral drugs and the like are overcome.

The invention belongs to the technical field of growing crop harvesting, and provides an integrated mulberry leaf harvester. The integrated mulberry leaf harvester comprises a frame and a collecting mechanism, and further comprises a leaf cutting mechanism which comprises two cutting assemblies, and each cutting assembly comprises a first transmission shaft, a chain wheel and a chain ring; the two shifting assemblies are symmetrically arranged on the left side and the right side of the front end of the frame; the leaf cutting mechanism comprises two cutting assemblies, each cutting assembly comprises a first transmission shaft, a chain wheel and a chain ring, the first transmission shafts are rotationally connected with the frame, the first transmission shafts are connected with a power device, a plurality of cutters are arranged on the chain rings, and the two stirring and gathering assemblies are used for stirring and gathering mulberry shoots to the position between the chain rings of the two cutting assemblies. According to the integrated mulberry leaf harvester, mulberry leaves can be automatically harvested, meanwhile, mulberry branches of mulberry trees are prevented from being damaged, the mulberry leaf production capacity of the harvested mulberry branches is guaranteed, and the yield of a mulberry field is guaranteed.

The invention discloses a method for interplanting sweet potatoes in a mulberry field, relates to the field of mulberry interplanting, and solves the problems of insufficient illumination of sweet potatoes, nutrient competition between mulberries and sweet potatoes and water and soil loss of ridges in a wide-narrow row mulberry field. The method comprises the steps that mulberries are planted in wide and narrow rows, an arc-shaped ridge face ridge is formed in the middle of the wide rows, and drainage ditches are formed in the two sides of the ridge; guiyuan No.10 sweet potato seedlings are planted on the ridges, and Guimulberry superior 12 mulberry seedlings are planted in narrow rows; sweet potatoes are planted 10-15 days after mulberries sprout in spring, the first-time mulberry harvesting height of the mulberries is 1.5-1.7 m, and the symbiotic period is 140-160 days; in the drought season, irrigation management is implemented, and the irrigation amount is 20-30 cubic meters per mu every time. The method can effectively improve the land utilization rate of the mulberry field and the comprehensive benefits of mulberry and sweet potatoes.

The present invention relates to a composition for improving cardiovascular calcification using a mulberry tree extract and a method for preparing the same. The composition is prepared by collecting the fruit (mulberry), leaves (mulberry leaves), branches (mulberry branches), and root bark (mulberry bark) of the mulberry tree, extracting the fruit and freeze-drying it to produce a mulberry extract, hot-air drying the leaves, branches, and root bark, adding 20-70% ethanol corresponding to 5-50% by weight of each, extracting at 50-90°C for 2-10 hours, filtering and concentrating the extract, and freeze-drying the extract to obtain a mulberry tree extract, and dissolving each extract in distilled water at a concentration of 10-50 mg / ml to provide a composition for improving cardiovascular calcification using a mulberry tree extract and a method for preparing the same, thereby enabling relatively safe and effective improvement of cardiovascular calcification, including vascular calcification.

The invention provides Pantoea ananas Pa-6 and an application of the Pantoea ananas Pa-6 as a mulberry bacterial wilt biocontrol bacterium and a bacterial fertilizer. The pantoea ananatis Pa-6 is preserved in Guangdong Microbial Culture Collection Center on June 19, 2025, and the preservation number is GDMCC NO: 66555. The strain has a good antagonistic effect on mulberry bacterial wilt pathogen lauracia solanacearum, and can be used for preventing and controlling mulberry bacterial wilt; the strain can promote germination of mulberry seeds, has the capabilities of dissolving potassium, producing auxin and producing siderophores, and can promote growth of mulberry trees; the method has an important value in the aspect of developing a microbial fertilizer which has the effects of preventing and treating mulberry bacterial wilt and promoting growth.

The present invention relates to the technical field of mulberry tree planting, and particularly relates to a fertilization and water management control method and system for mulberry tree planting. The method includes the following steps: collecting multi-source data of the mulberry tree planting area and performing spatio-temporal alignment preprocessing to obtain spatio-temporal alignment preprocessed data; extracting a regional soil data layer according to the spatio-temporal alignment preprocessed data to obtain a regional soil data layer; constructing a multi-source data layer according to the regional soil data layer and the spatio-temporal alignment preprocessed data to obtain a multi-source data layer; constructing a soil-mulberry tree system model according to the multi-source data layer and constructing a spatio-temporal dynamic database to obtain a spatio-temporal dynamic soil-mulberry tree system database. The present invention constructs a soil-mulberry tree system model and a spatio-temporal dynamic database, and combines the management zoning technology to realize digital and model-driven precise fertilization and water management, and achieve the goals of efficient utilization of water and fertilizer resources and water saving and yield increase.

The present invention discloses a planting and cultivation method for simultaneously producing mistletoe and mulberry fruits, comprising the steps of (1) selecting and preparing a site: selecting a flat plot with fertile soil and convenient picking and drainage and irrigation, and digging planting holes after deep plowing, with deep trenches around the plot; (2) planting mulberry trees: selecting high-quality fruit mulberry seedlings for planting; (3) cultivating after planting: after the fruit mulberry seedlings germinate, selecting strong branches in different directions, and pinching the tops when the new shoots reach 35 to 45 cm in length; (4) parasitizing: adhering mistletoe seeds to the internodes of mulberry branches, and spraying foliar fertilizer after parasitizing; (5) water and fertilizer management; and (6) harvesting. The cultivation method of the present invention can meet the requirements of artificially cultivated host plants for mistletoe medicinal materials by using high-quality fruit mulberry trees as host plants for producing mistletoe, and realizes the development of a mulberry fruit specialty industry chain, integrating economic, ecological and social benefits; and the cultivation method is simple to operate, has low planting costs, and has good economic benefits.

The invention discloses a fruit cudrania tricuspidata shaping and pruning method which comprises the following steps: field planting: annual fruit cudrania tricuspidata grafting seedlings are required, and field planting is performed in mid-early March; in the growing season, other germinated buds are removed, and a branch growing vigorously and fully is reserved on the top to serve as a center trunk; when the central trunk grows to 1.8-2.0 m, terminal buds are removed, and primary lateral branches are reserved at the position of 1.7-1.9 m; second-stage lateral branch cultivation: in the growing season of the second year, the first-stage lateral branches continue to grow in a prolonged mode, and when the total length of the first-stage lateral branches reaches 1.7-2.0 m, terminal buds of the first-stage lateral branches are removed, and growth of the second-stage lateral branches is promoted; third-stage lateral branch cultivation: during pruning in the dormancy period every year, properly retaining fruiting branches, and cultivating third-stage lateral branches. According to the fruit cudrania tricuspidata shaping and pruning method, the fruit yield is increased, and the yield of a single plant is 60-100 kg; a layer of branches is formed, so that fruits are convenient to pick, the influence of cudrania tricuspidata thorns on picking personnel is avoided, and the picking efficiency is improved.

The invention discloses Burkholderia cepacia ZJ15, a microbial fertilizer and application of the Burkholderia cepacia ZJ15 and the microbial fertilizer in the aspect of preventing and treating diseases of mulberry trees and oak trees. The strain is preserved in Guangdong Microbial Culture Collection Center on June 05, 2025, and the preservation number is GDMCC No: 66469. The bacterial strain not only can promote germination of mulberry seeds, but also has the capabilities of dissolving inorganic phosphorus, dissolving organic phosphorus, producing auxin, producing siderophores and fixing nitrogen, can promote growth of mulberries, and has a relatively comprehensive growth promoting function; in addition, the bacterium has a relatively good antagonistic effect on mulberry and oak pathogenic bacteria such as colletotrichum truncatum, ailanthus guaiensis, enterobacter cloacae and the like; therefore, the bacterial manure can be developed into bacterial manure with the effects of preventing and treating diseases and promoting growth, and has good application prospects and application values in the aspects of preventing and treating diseases of mulberry trees and oak trees and promoting growth.

The application discloses a mulberry tree planting mode adaptive matching system, which comprises a park coordination node, a land end device, a field communication network and a planting rule library. Based on the mulberry tree planting mode library and the phenological window, the system retrieves a candidate mode according to microenvironment clustering, issues a parameter range, collects specific indexes such as leaf temperature and leaf area index, and updates the template according to the feedback variable closed loop. The field edge node identifies transplanting stress or soil water potential anomaly and triggers emergency rearrangement in linkage with ecological load; and in a weak network scene, supports combined reporting and idempotent processing. The system improves the survival rate and post-harvest recovery, and reduces the energy consumption and mode misfit risk.

The utility model relates to the technical field of mulberry felling equipment, in particular to a mulberry felling device and a mulberry felling machine, which comprise a pressing rod frame, a lifting frame, a mulberry felling mechanism and a transmission mechanism, the lifting frame can be installed on the pressing rod frame in a lifting mode. The mulberry cutting mechanism is mounted on the lifting frame and comprises a pair of saw discs which can rotate and are matched with each other; the transmission mechanism is installed in the lifting frame, and the output end of the transmission mechanism can drive the two saw discs to rotate synchronously at the same time. According to the mulberry cutting device and the mulberry cutting machine, mulberries are pressed and laid down through the pressing rod frame, so that the roots of the mulberries are bent, and cutting of the pair of saw discs is facilitated; the arrangement of the lifting frame facilitates the adjustment of the height of the saw disc to meet the requirements of different cutting heights. And the two saw discs are arranged and matched with each other, so that the mulberry trees can be stressed on the two sides at the same time in the cutting process, the stability of the mulberry trees after lodging is improved, and collection of the mulberry twigs in the later period is facilitated.

The present invention discloses an automatic mulberry leaf picking device from top to bottom, which includes a transverse movement mechanism, a collection mechanism, a picking mechanism, and a clamping mechanism; a first lifting platform, a second lifting platform, and a third lifting platform are respectively arranged on the collection mechanism, the picking mechanism, and the clamping mechanism, and a gear rack mechanism is installed on each side of each lifting platform. The structures of the three lifting platforms are completely the same, and they are all connected to the lifting guide rail to realize lifting; a collection box is installed on the lower left rod and the lower right rod of the collection mechanism to complete the collection of mulberry leaves; when the three rods of the picking mechanism are closed, the single-sided steel needle plate and the double-sided steel needle plate cooperate with each other to form a plurality of cylindrical picking channels; a spring is provided on the clamping component of the clamping mechanism, so that the clamping mechanism can clamp mulberry branches of different shapes and sizes; the mulberry leaf picking device can realize the picking and collection of all mulberry leaves of a single mulberry tree and has a certain protective effect on mulberry branches.

The invention relates to the technical field of plant biology, and particularly discloses a preparation and instantaneous conversion method of mulberry protoplast. The preparation method comprises the following steps: taking young leaves of mulberry tissue culture seedlings as materials, carrying out light-proof enzymolysis by adopting an optimized enzymatic hydrolysate combination, and then filtering, centrifuging and washing to obtain the high-activity protoplast. According to the instantaneous transformation method, exogenous plasmids are introduced into protoplasts by using a specific PEG / Ca < 2 + > transformation solution. According to the method, the interference of mulberry leaf polysaccharide polyphenol is effectively overcome by optimizing an enzymolysis system, and the yield and survival rate of the protoplast are remarkably improved; the instantaneous transformation efficiency is remarkably improved by improving a transformation system, and an efficient and stable single cell research platform is provided for gene function research and molecular breeding of mulberry trees.

The application discloses a strain of Pseudomonas extremorientalis LJ-6 and application of the strain in preventing and controlling mulberry diseases. The Pseudomonas extremorientalis in the application is preserved in the Guangdong Microbial Culture Collection Center on June 18, 2024, and the preservation number is GDMCC NO: 64766. The Pseudomonas extremorientalis LJ-6 has good antagonistic effects on bacterial pathogenic bacteria such as Caulobacter segnis, Pantoea ananatis, Klebsiella michiganensis and Enterobacter cloacae, and fungal pathogenic bacteria such as Fusarium oxysporum, Curvularia lunata and Guignardia bidwellii. The Pseudomonas extremorientalis LJ-6 has good prevention and control effects on plant diseases caused by the above pathogenic bacteria invading plants, such as mulberry bacterial wilt, mulberry bacterial blight, mulberry leaf spot and mulberry anthracnose. Therefore, the Pseudomonas extremorientalis LJ-6 has good application prospect and value in preventing and controlling plant diseases such as mulberry and preparing disease prevention and control preparation products.

The utility model relates to mulberry tree planting technical field discloses a kind of mulberry tree seedling planting support, including bottom net, the frame structure is equipped with outside bottom net, the bottom net upper end face edge is fixedly connected with net rack, six support rods are equipped with in the net rack upper end, six the support rod upper end is fixedly connected with support plate, six supporting structures are arranged in the round of support plate upper end surface, to one of the supporting structures as an example, the supporting structure includes support shell, the storage cavity is arranged in the center of one end surface of support shell near support plate center, non-newtonian fluid is arranged in the storage cavity, non-newtonian fluid one side near support plate center one end is equipped with piston. In the utility model, non-newtonian fluid can produce different damping effect according to the size of extrusion pressure, and cooperate with sponge pad and liquid shaking to form opposite phase wave, shock absorption is carried out, and the influence caused by shaking and wind to branch is reduced.

The present invention discloses a method and system for identifying and analyzing salt-tolerant mulberry varieties based on artificial intelligence, including: obtaining mulberry tree image data of different mulberry varieties at each growth stage and performing preprocessing, extracting attribute features and performing category division to construct a first topological structure diagram; obtaining growth image data of each mulberry variety in different physiological environments, performing stress feature analysis and constructing a second topological structure diagram; evaluating the salt tolerance of various mulberry trees based on the first topological structure diagram and the second topological structure diagram to obtain salt tolerance evaluation information; constructing a mulberry variety identification and analysis model, using the first topological structure diagram, the second topological structure diagram and the salt tolerance evaluation information to establish a mulberry knowledge graph and perform model training, identifying and analyzing the mulberry trees to be identified, and determining whether they are suitable for planting in the expected planting area. The accuracy and comprehensiveness of the identification of salt-tolerant mulberry varieties are improved and the breeding and identification cycle is reduced.

The invention discloses Trichoderma asperellum TR41 for antagonizing pathogenic bacteria of mulberry bacterial wilt and application of the Trichoderma asperellum TR41, the Trichoderma asperellum TR41 is identified as Trichoderma asperellum, the Trichoderma asperellum TR41 is preserved in Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC No.64887, and the preservation time is July 18, 2024. Trichoderma TR41 with a good antagonistic effect on the mulberry ralstonia solanacearum is separated and identified, the Trichoderma TR41 is identified as trichoderma asperellum through morphological identification and molecular biology, meanwhile, it is determined that the bacteriostatic activity of the TR41 on the mulberry ralstonia solanacearum is as high as 97.5%, a biological membrane of the mulberry ralstonia solanacearum can be damaged, the permeability of the biological membrane is increased, and the Trichoderma TR41 has a good antagonistic effect on the mulberry ralstonia solanacearum. And the exercise ability of the mulberry ralstonia solanacearum is inhibited. In addition, TR41 does not affect the growth and development of mulberry trees, and shows the safety of TR41 as a biocontrol strain.

The application discloses a parallel clamping type automatic mulberry leaf picking device, which comprises a base, a rack movably arranged on the base along the front-back direction, a lifting platform movably arranged on the front side of the rack along the up-down direction, a clamping assembly movably arranged on the lifting platform and used for synchronously clamping each branch, a pair of collecting assemblies respectively arranged on the left and right sides of the clamping assembly and used for collecting leaves, a pair of picking assemblies respectively arranged on the left and right sides of the clamping assembly and used for cutting leaves on the branches, and a lifting assembly arranged between the rack and the lifting platform and used for driving the clamping assembly to move along the up-down direction of the rack. The device aims at realizing one-time picking and collecting of mulberry leaves of multiple branches of a single mulberry tree and greatly improving the picking efficiency of mulberry leaves.

The present invention discloses a detection kit for Pseudomonas amylovora, Pseudomonas syringae, and / or mulberry blight. Based on the gene encoding a hypothetical protein of the mulberry blight pathogens Pseudomonas amylovora and / or Pseudomonas syringae, as shown in SEQ ID NO: 1, a primer set for detecting the mulberry blight pathogens Pseudomonas amylovora, Pseudomonas syringae, and / or mulberry blight is designed. The primer set is used for PCR and LAMP detection. The nucleotide sequences of the primer set for PCR detection are shown in SEQ ID NOs: 8-9, and the nucleotide sequences of the primer set for LAMP detection are shown in SEQ ID NOs: 32-35. The primer set has high specificity and sensitivity, and the reaction results can be identified by the naked eye. The PCR and LAMP detection kit containing the primer set is used for rapid detection of the infectious mulberry blight pathogens Pseudomonas amylovora, Pseudomonas syringae, and / or mulberry blight in mulberry trees and mulberry seedlings in mulberry gardens.

This invention belongs to the field of pesticide technology, specifically to a pesticide formulation for controlling powdery mildew on mulberry trees. The pesticide formulation for controlling powdery mildew on mulberry trees uses a binary compound of copper oxychloride and pyraclostrobin or fluopyram as its active ingredient. The active ingredient in this pesticide formulation has a significant synergistic effect, which can improve efficacy, reduce dosage, lower disease control costs, and reduce pesticide residues and environmental pollution.