19 results about "Ananas" patented technology

The present application relates to a kind of anaphalis contorta exosome extraction method and its application.The exosome is obtained by ultra-high speed centrifugation method from fresh anaphalis contorta, particle size is 160-200 nm, Zeta potential is-21.63±1.34 mV, with double membrane structure, mainly containing protein (such as resveratrol O-methyltransferase etc.) and phospholipid, polysaccharide content is only 12.5% of anaphalis contorta fresh medicine, and does not contain traditional active ingredient anaphalis contorta glycoside.Experiments show that anaphalis contorta exosome can be targeted to enrich in colorectal tissue, significantly improve AOM / DSS induced colorectal cancer model mouse tumor microenvironment, reduce tumor number, regulate T cell and macrophage ratio, and relieve pathological damage, better than anaphalis contorta fresh medicine.The mechanism is related to protein component, not polysaccharide or small molecule component.The anaphalis contorta exosome provided in the present application has simple preparation process, high safety, and has significant anti-colorectal cancer application potential.

Disclosed is a breeding method for cultivating a cold-resistant and high-temperature-resistant Ananas comosus germplasm. The breeding method mainly comprises the following technical key points: creating a controllable greenhouse environment which simulates a natural environment for production, performing low-temperature domestication culture in two stages of seedling planting stage and breeding stage in a growth period, and performing high-temperature domestication culture in multiple stages of seedling growth stage, flowering stage, fruit stage and breeding stage, and for each key stage according to a growth pattern of Ananas comosus, providing precise technical implementation key points on time point control and management measure digitalization, so as to facilitate accurate implementation of operations. The method is simple and convenient for large-scale breeding production, multiple rounds of seedlings can be harvested in one round of production, and the seedling production efficiency of the cold-resistant and high-temperature-resistant Ananas comosus germplasm is multiplied.

The invention relates to the field of postharvest after-ripening of agricultural products, and discloses a single-fruit ripening method of annona squamosa, which comprises the following steps: placing a single annona squamosa fruit and an ethylene-loaded ripening agent in a contact state; wrapping and sealing the two by using a packaging system; and putting the sealed packaging body at a proper temperature for ripening. Preferably, the ripening agent is formed by adsorbing 2.0%-3.0% of ethylene by activated carbon, and the dosage of the ripening agent is 0.3 g-0. 4g; the packaging system comprises two layers of absorbent paper and a PE self-sealing bag provided with four holes of 0.20-0.30 cm < 2 >. The proper temperature is 19-21 DEG C, and the ripening time is 1-2 days. Through the synergistic effect of the ripening agent and a specific packaging system, a ripening microenvironment integrating ethylene enrichment, gas exchange and humidity regulation and control is constructed, and the technical problems that annona squamosa ripening is slow, and annona squamosa is prone to rot are solved. The method is high in ripening speed, high in fruit quality, simple and convenient to operate, and time-saving and labor-saving.

The present utility model relates to a composition for pickled pineapple intended for human consumption. The composition comprises 40% by weight pineapple, 8% by weight carrot, 8% by weight bell pepper, 2% by weight ginger, 2% by weight onion, 2% by weight garlic, 22% by weight vinegar, 12% by weight sugar, 1% by weight black pepper, and 3% by weight raisins. The combination of these ingredients produces a pickled food product with a balanced sweet, sour, and mildly spiced flavor. The resulting pickled pineapple provides an improved vegetable-fruit pickle suitable as a condiment or side dish and is appropriate for local food production and commercialization.

The invention relates to the technical field of modern agricultural planting, in particular to a method for improving the quality of field pineapples based on coordinated regulation and control in the whole growth period. The method comprises the following steps: applying a composite bio-organic fertilizer before planting or in the early stage of a vegetative growth period; in the squaring stage, a flower promoting and strengthening nutrient solution is sprayed to leaf surfaces once; diluted trace element fertilizer is sprayed to leaf surfaces for one time 7 days after flower falling and 20-25 days after flower falling; starting from 55-65 days after flower withering, spraying a potassium improver to leaf surfaces for 1-2 times at an interval of 10-15 days; when 5-10% of the fruit peels turn color, a color-turning sweetener is sprayed once; in the fruit color changing period, if continuous cloudy and rainy weather occurs, a silver reflective mulching film is laid to increase illumination of the lower portion of a tree crown, and uniform coloring of fruits is promoted; if the fruit color changing period is in a high-temperature drought season, and the highest air temperature of the day is continuously higher than 35 DEG C, a sunshade net with the shading rate being 20-30% is adopted for shading at 11 o'clock to 14 o'clock every day.

The utility model discloses a tissue culture rapid propagation device for anoectochilus formosanus, which comprises a placing component, and the placing component comprises a movable frame, a movable block, a connecting column, a mounting column, a limiting frame and a storage frame. Through the arrangement of the placing assembly, when a culture bottle needs to be taken, pulling force is firstly applied to the guide rod, so that the spring is in a compressed state, then the limiting frame is inserted into the mounting column, the force applied to the guide rod is cancelled, and the limiting column is inserted into the limiting frame and the mounting column under the elastic force action of the spring; according to the scheme, the storage frame is limited to the top of the mounting column, pushing force is applied to the storage frame, the movable block is driven to move in the movable frame, the storage frame is moved to the needed position, one hand drags one end of the storage frame, and the other hand places the corresponding culture bottle in the storage frame. Therefore, the culture bottle needing to be observed can be taken out more accurately and stably.

Disclosed is a sweet and spicy pineapple (Ananas comosus) peel vinegar formulation that addresses the need for a value-added food product utilizing pineapple peel, an agricultural by-product, while providing a naturally preserved flavored vinegar. The formulation comprises fermented pineapple peel vinegar and a flavoring composition including salt, black pepper, garlic, onion, chili, and sugar. The formulation exhibits a pH of less than 3.5 and is free of added chemical preservatives, thereby providing a shelf-stable condiment with desirable sweet, acidic, and spicy sensory characteristics. By combining fermented pineapple peel vinegar with a defined flavoring composition, the formulation enhances the culinary versatility and commercial value of pineapple peel-derived vinegar while promoting the beneficial utilization of fruit processing waste.

The application provides a method for promoting the growth of an anthurium andraeanum root system. The method for promoting the growth of the anthurium andraeanum root system is characterized in that the anthurium andraeanum plant is subjected to root irrigation with an anthurium andraeanum root growth-promoting liquid to soak the roots of the anthurium andraeanum plant, and the anthurium andraeanum root growth-promoting liquid is an endophytic fungal culture solution of an orange-yellow calanthe. The method for promoting the growth of the anthurium andraeanum root system can effectively promote the growth of the anthurium andraeanum root system, and further promote the rapid growth of the anthurium andraeanum plant.

The utility model discloses a breadfruit positioning peeling device relates to agricultural product processing equipment technical field, including base, support frame, drive seat, clamping piece, cutting assembly, support frame is fixed in base, and drive seat rotatory installation is in base, and rotates relative to base through drive arrangement, clamping piece is installed in the top of drive seat, is used for clamping the lower part of the breadfruit of waiting peeling, when drive seat rotates, and the breadfruit synchronous rotation is driven, the slide rod sliding of cutting assembly is installed in support frame, and the arc blade is fixed in the bottom of slide rod, and when cutting, slide rod moves down, makes the arc blade cut the upper part of breadfruit, realizes the peeling operation of breadfruit, realizes stable positioning and accurate peeling, promotes processing efficiency and pulp utilization, uses more safely.

The application discloses an AaHDS gene of ananas comosus and application of the AaHDS gene in regulating aroma, and belongs to the field of genetic engineering of plants. The AaHDS gene of the ananas comosus is obtained by PCR amplification and cloning, the DNA sequence of the gene in the ananas comosus with aroma and the ananas comosus without aroma has obvious difference, and the expression amount of the gene is the highest in the inflorescence. The VIGS technology is used to silence the gene, the expression amount of the gene is reduced by 49.1 %, and the contents of alpha-pinene, beta-pinene and eucalyptol are significantly reduced. The AaHDS gene cloned by the application is beneficial to genetic improvement of plant aroma characteristics, and can be used for cultivating more new varieties of the ananas comosus with pleasant aroma.

The application provides a dynamic decision method and system for soilless cultivation of Anoectochilus roxburghii, and relates to the field of agricultural intelligence technology.The method comprises the following steps: in step 2, based on multi-dimensional environmental data and plant physiological indexes, the time sequence physiological indexes are analyzed, and the dynamic change mode characteristics of the stage change of the physiological indexes are extracted; the shape characteristics of different time segments are matched and fused, and the current growth stage of Anoectochilus roxburghii is dynamically recognized through a preset growth stage recognition model; in step 3, according to the current growth stage of Anoectochilus roxburghii, the dynamic ideal threshold range corresponding to the current stage is obtained from the preset growth stage and environmental mapping relationship.The application realizes self-adaptive regulation and control of the soilless cultivation environment, and improves the growth quality and yield.

Disclosed is a process for producing a sweet and spicy pineapple (Ananas comosus) peel vinegar that addresses the need for value-added utilization of pineapple peel as an agricultural by-product while producing a naturally preserved flavored vinegar. The process comprises preparing a fermentation mixture of pineapple peels and a sugar solution, fermenting the mixture under controlled temperature conditions, separating and further fermenting the resulting liquid, filtering the fermented liquid, mixing the filtered liquid with flavoring agents comprising salt,black pepper, garlic, onion, chili, and sugar, and heating the resulting mixture. The process produces a flavored pineapple peel vinegar having a pH of less than 3.5, thereby providing a shelf-stable vinegar without added chemical preservatives while retaining the characteristic properties of pineapple peel vinegar and enhancing its culinary acceptability.

The utility model discloses a simple support for under-forest cultivation of anoectochilus formosanus, which comprises a horizontal top rod, a vertical rod, a plurality of vertical rods, a plurality of horizontal mounting rods and a plurality of horizontal supporting rods, one end of the top rod is connected with a hoop, the other end of the top rod is detachably connected with the top of the vertical rod, and the lower end of the vertical rod is a tip. A plurality of vertically-through first square holes are formed in the ejector rod in the length direction, the vertical rods are square pipes used in cooperation with the first square holes, the upper ends of the vertical rods are inserted into the first square holes, a plurality of transversely-through second square holes are formed in the vertical rods in the length direction, and the installation rods are square pipes used in cooperation with the second square holes and inserted into the second square holes. The installation rod is perpendicular to the top rod and the vertical rod, at least four transverse through holes are formed in the installation rod in the length direction, the supporting rods are used in cooperation with the through holes, inserted into the through holes and used for supporting the anoectochilus formosanus cultivation pots, and the supporting rods are parallel to the top rod. The anoectochilus formosanus cultivation device has the advantages that under-forest resources are fully utilized, and the anoectochilus formosanus

The application provides a propagation method for tissue culture of anthurium andraeanum, which comprises the following steps: external explant selection and disinfection, external explant cutting, callus induction, proliferation body cultivation, differentiation of adventitious buds, rooting and strong root training, seedling training and transplantation. The composite disinfectant is composed of chlorobromoisocyanuric acid, everlasting flower oil, Tween and distilled water in a specific ratio. Chlorobromoisocyanuric acid and everlasting flower oil produce complementary effects through different antibacterial mechanisms, thereby improving the broad-spectrum and bactericidal effect of the composite disinfectant. Sambucus nigra extract is added to the rooting culture medium, and the coumarin and ferulic acid contained in the sambucus nigra extract can be converted into lignin in the anthurium andraeanum tissue culture seedlings, thereby enhancing the mechanical connection between cells and making the connection between the adventitious roots and the callus more compact. In addition, the polysaccharides and their derivatives contained in the sambucus nigra extract can promote the synthesis and deposition of pectin in the tissue culture seedlings, thereby increasing the adhesion between cells and making the connection between the adventitious roots and the callus more compact.

The application provides a kind of anaphalis contorta cluster bud transplanting cultivation and circulatory cutting efficient breeding method, relates to the field of anaphalis contorta cluster bud transplanting, including the disinfection of selected anaphalis contorta plant, after the disinfection and washing of anaphalis contorta plant with sterile water, the stem segment of 2-3cm length with node is cut, is inoculated to cluster bud germination medium and is induced to obtain anaphalis contorta cluster bud;Anaphalis contorta cluster bud grows to 2-3cm and is cut into 0.5-1cm tissue block and is transferred to proliferation growth medium, and is cultured under full light;Anaphalis contorta cluster bud grows to 5-6cm and is transplanted outdoors, is planted in straw debris and peat mixed matrix, and anaphalis contorta cluster bud grows to 8-10cm and is cut with root hair node segment for cutting, and cutting matrix is straw debris and peat mixed matrix, and anaphalis contorta cutting plant grows to 8-10cm and is cut for expanding propagation seedling;Cutting anaphalis contorta seedling is used for large-scale production transplanting when growing to 3-4cm.The present application is conducive to the full contact and attraction of external plant to the components in the induction medium, promotes the maturation of plant, thereby improving the survival rate of transplanting and seedling raising.

The invention relates to the technical field of plant tissue culture, in particular to a sterile sowing method for Hainan anoectochilus formosanus, which specifically comprises the following steps: selecting mature seed clips of Hainan anoectochilus formosanus as explants, and disinfecting; inoculating the disinfected seed clamp into a germination culture medium for germination; inoculating somatic embryos obtained by germination into a somatic embryo induction culture medium for induction; inoculating the induced budlets into a proliferation culture medium for proliferation culture; and inoculating the Hainan anoectochilus formosanus cluster buds obtained by multiplication culture into a rooting culture medium for rooting culture to obtain rooted seedlings. According to the method, the mature seed clamp of the Hainan anoectochilus roxburghii is used as the explant, sterile sowing of the Hainan anoectochilus roxburghii is carried out through an in-vitro rapid propagation method, large-scale rapid propagation of the Hainan anoectochilus roxburghii is achieved, the problem that the propagation rate of traditional rhizome propagation is low is solved, and the method has important significance on large-scale planting, popularization and utilization of the Hainan anoectochilus roxburghii.

Disclosed is a method for inducing field-planted Ananas comosus to rapidly propagate seedlings, which adopts a new technical management method for practical production of the field-planted Ananas comosus, and comprises: pruning and shaping mother plants of Ananas comosus, performing sterilization and pest prevention on the mother plants of Ananas comosus, promoting germination, performing fertilization, and propagating and culturing strong seedlings, especially implementing application management of plant kinetin, so that a seedling propagation period of the field-planted Ananas comosus is shortened by more than 60 days compared with a natural propagation period, and by means of 2-3 rounds of propagation and harvesting, a propagation coefficient of a single mother plant is improved to reach 12. The method has a high propagation rate, realizes rapid production of a large number of native offset seedlings of Ananas comosus, and greatly improves the production efficiency of Ananas comosus, and reduces a seedling propagation cost.

A breeding method for cultivating a cold-resistant and high-temperature-resistant Ananas comosus germplasm comprises the following technical key points: creating a controllable greenhouse environment which simulates a natural environment for production, performing low-temperature domestication culture in two stages of seedling planting stage and breeding stage in a growth period, and performing high-temperature domestication culture in multiple stages of seedling growth stage, flowering stage, fruit stage and breeding stage, and for each key stage according to a growth pattern of Ananas comosus, providing precise technical implementation key points on time point control and management measure digitalization, so as to facilitate accurate implementation of operations. The method is simple and convenient for large-scale breeding production, multiple rounds of seedlings can be harvested in one round of production, and the seedling production efficiency of the cold-resistant and high-temperature-resistant Ananas comosus germplasm is multiplied.

The present application relates to a kind of gold pteridophyte 'Jin Kang No.1' WRKY transcription factor ArWRKY6, coding gene and its application, above-mentioned transcription factor ArWRKY6 includes the polypeptide with the amino acid sequence as shown in SEQ ID NO.2.The present application also provides a kind of nucleotide sequence SEQ ID NO.1 for encoding above-mentioned protein.The present application provides a theoretical basis for promoting gold pteridophyte to bloom in advance, promote leaf and seed development using genetic engineering technology, has important application value.