The invention provides a formula of an induction medium for efficiently inducing anther culture of japonica rice. The formula of the induction medium comprises the following components in certain proportions: KNO3, (NH4)2SO4, KH2PO4, CaCl2.2H2O, MgSO4.7H2O, Na2-EDTA, FeSO4.7H2O, MnSO4.4H2O, ZnSO4.7H2O, H3BO3, KI, glycerol, Na3C6H5O7.H2O, folic acid, glycine, alanine, lysine, vitamin B1, vitamin B6, niacin, 2-hydroxyethyl trimethyl ammonium chloride, sucrose, plant gel, 2,4-D, NAA, phthalocyanine complex nucleic acid, protolysate, activated carbon, taurine, biotin and plant sulfide kinetin alpha. The induction medium has the advantages that callus of anther of japonica rice can be efficiently induced, the browning can be lowered, and the culture efficiency of the anther of japonica rice can be substantially improved.