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333 results about "Kinetin" patented technology

Kinetin (/'kaɪnɪtɪn/) is a type of cytokinin, a class of plant hormone that promotes cell division. Kinetin was originally isolated by Miller and Skoog et al. as a compound from autoclaved herring sperm DNA that had cell division-promoting activity. It was given the name kinetin because of its ability to induce cell division, provided that auxin was present in the medium. Kinetin is often used in plant tissue culture for inducing formation of callus (in conjunction with auxin) and to regenerate shoot tissues from callus (with lower auxin concentration).

Plant sprouting agent and preparation method thereof

The invention relates to a plant sprouting agent and a preparation method thereof. The materials of the sprouting agent are 6-benzyladenine, kinetin, gibberellin, dimethylsulfoxide, glycerol and lanolin; the dimethylsulfoxide solution and the glycerol are added into a glass beaker and dissolved, so that hormone solution is obtained; the lanolin is put into another big beaker, and is heated to 70 DEG C to be melted, and as the lanolin is stirred to be slowly melted, the hormone solution is slowly added into the lanolin and sufficiently and uniformly stirred; and after being cooled to the room temperature, the hormone mixture is coagulated into paste, which is the high-effective plant sprouting agent. Each component in the sprouting agent can rapidly penetrate the cell membranes of the dormant buds of plants and directionally enter cells, consequently, the efficacy is enhanced, and the acting time is shortened. The plant sprouting agent can increase the plant sprouting speed, shorten the growing time and accelerate seedling formation; bud dormancy is broken, so that the growth of plants is increased; the plant sprouting agent can change the hormonal balance in plant cells, promote sprouting and accelerate the elongation growth of the stems and leaves of plants; and the plant sprouting agent has a moisture-keeping effect, which can help to protect the new sprouts of plants.
Owner:HENAN UNIV OF SCI & TECH

Efficient tissue culture and rapid propagation technology for seedlings of bletilla striata

The invention discloses an efficient tissue culture and rapid propagation technology for seedlings of bletilla striata. According to the technology, bletilla striata seeds are sown on a seed germination culture medium for sterile germination, the obtained seedlings are inoculated to a multiplication and subculture medium one by one for multiplication and subculture, clustering seedlings are obtained and inoculated to a rooting culture medium for rooting culture, the obtained tissue culture seedlings are planted on a prepared seedling hardening substrate, and the seedlings are transplanted to a large filed for plantation after seedling hardening, wherein the seed germination culture medium comprises MS (magnesium sulfate), 30g/L of sugar, 8g/L of agar and 10g/L-12g/L of potatoes; the multiplication and subculture medium comprises MS, 1.0 mg/L of KT (kinetin), 0.2 mg/L of NAA (naphthalene acetic acid), 30g/L of sugar and 9g/L of agar; the rooting culture medium comprises MS, 0.2 mg/L of NAA, 1.0mg/L of IAA (indole acetic acid), 30g/L of sugar, 9g/L of agar and 25-30 g/L of potatoes; the seedling hardening substrate comprises medium moor peat soil, perlite and wood dust in the volume ratio being 5:2:3. The technology is mature, a system is perfect, the problems of low natural germination rate of bletilla striata and difficulty in obtaining of seedlings can be effectively solved, and the technology has better popularization and application value and broad market prospect.
Owner:HENAN UNIV OF SCI & TECH

Culture medium for in-vitro induced regeneration plants of double-haploid stems of potatoes

InactiveCN102870682ASolve the key technical problems of low regeneration rateImprove seedling ratePlant tissue cultureHorticulture methodsEthylenediamineSucrose
The invention discloses a culture medium for in-vitro induced regeneration plants of double-haploid stems of potatoes, which belongs to the field of agricultural biotechnologies. The culture medium comprises the following compositions: potassium nitrate, ammonium nitrate, monopotassium phosphate, magnesium sulfate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, copper sulfate, cobalt chloride, ferrous sulfate, disodium ethylenediamine tetraacetic acid, nicotinic acid, pyridoxine hydrochloride, glycine, glutamine, casein hydrolysate, yeast extracts, sucrose, 6-benzylaminopurine, naphthalene acetic acid, kinetin, zeatin and heteroauxin beilining. The culture medium is applied to the induction and culturing of double-haploid stem regenerated seedlings of potatoes, the test effect is good, the initiating rate is increased by 76%, and the seedling rate is as high as 80.5%. The regeneration rate of double-haploid stems of potatoes is significantly increased, thereby laying a foundation for the establishment of a genetic transformation system; and the culturing mode adopts a one-step seedling method, so that the pollution probability is reduced.
Owner:VEGETABLE RES INST OF SHANDONG ACADEMY OF AGRI SCI

Method for enlarging oriental lily scale cutting ball

The invention discloses a method for enlarging an oriental lily scale cutting ball. The method comprises the steps as follows: from the last ten-day period of August to the beginning of September, selecting a detoxified tissue culture ball after low temperature vernalization or a healthy and strong imported seedball without pest and disease damage such as oriental lily-sorbonne and the like as the cutting ball; selecting peat and perlite with the ratio of 1 to 1, adding the peat and the perlite into compound fertilizer, fully and uniformly mixing to serve as a cutting matrix, and disinfecting the matrix; and flatly spreading a scale, storing, soaking with cytokinin, disinfecting and drying. A layer of the matrix and a layer of the scale are placed into a basket for bud forcing treatment, and an exhaust fan is regularly started to promote the circulation of air in a culture room. According to the method, the seedball after low temperature vernalization is adopted for scale cutting at a proper temperature, so that the propagation coefficient of oriental lily scale cutting is increased; the scale is soaked with the cytokinin, so that a scale ball can be promoted to grow roots and the number of the roots is increased; and the nutritional level of the matrix is increased while the breathability and the water permeability of the matrix structure is not changed, the nutrition is provided for the rooting scale ball, and the expansion speed of the scale ball is increased.
Owner:LIANYUNGANG ACAD OF AGRI SCI

Tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts

InactiveCN102577970AHigh rate of callus inductionHigh rate of adventitious bud differentiationPlant tissue cultureHorticulture methodsSucroseCarrageenan
The invention discloses a tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts. The medium comprises at least one of the following mediums: a callus induction medium which comprises B5, 2mg/L of 6-BA (6-benzylaminopurine), 2mg/L of KT (kinetin), 0.1mg/L of NAA (naphthylacetic acid), 5.5-6.0g/L of carragheenan and 30g/L of sucrose and has a pH value of 5.6-5.8; an adventitious bud differentiation medium which comprises B5, 1mg/L of 6-BA, 2mg/L of KT, 0.1mg/L of NAA, 5.5-6.0g/L of carragheenan and 30g/L of sucrose and has a pH value of 5.6-5.8; an adventitious bud subculture medium which comprises MB, 1mg/L of 6-BA, 0.8mg/L of IAA (indoleacetic acid), 5.5-6.0g/L of carragheenan and 30g/L of sucrose and has a pH value of 5.6-5.8; and a rooting medium which comprises 1/3MB, 1.5mg/L of IBA (indolebutyric acid), 0.1mg/L of NAA, 5.5-6.0g/L of carragheenan and 20g/L of sucrose and has a pH value of 5.6-5.8. The medium of the invention, which has the advantages of high callusinductivity, high dventitious bud differentiation rate and propagation coefficient, high rooting rate, high transplanting survival rate of test tube sprouts, robust sprout growth, normal leaf shape and leaf color, rapid subcutaneous rooting and new leaf germination, and the like, can satisfy needs of the large-scale cultivation of Lonicera macranthoides Hand. Mazz Yulei No.1, and has a good application prospect.
Owner:CHONGQING UNIV OF ARTS & SCI

Populus alba * pobulus davidiana dode 1333 tissue culture medium of pobulus davidiana dode improved variety

ActiveCN103636498AAccelerate the process of promoting improved varietiesSpeed ​​up the promotion processHorticulture methodsPlant tissue culturePurineBud
The invention relates to a populus alba * pobulus davidiana dode 1333 tissue culture medium of an pobulus davidiana dode improved variety, which is used for solving a problem that the existing culture medium is poor in in-vitro rapid propagation effect for populus alba* pobulus davidiana dode 1333 regeneration tissue of hybrid offspring pobulus davidiana dode improved variety of pobulus davidiana dode and populus alba. The populus alba* pobulus davidiana dode 1333 tissue culture medium of the pobulus davidiana dode improved variety comprises an adventitious bud differentiation culture medium, a subculture multiplication culture medium and an adventitious bud rooting culture medium, wherein the adventitious bud differentiation culture medium consists of a YS (Eosine Yellowish) culture medium, 6-benzyl amido purine and kinetin; the subculture multiplication culture medium consists of the YS culture medium, the 6-benzyl amido purine and naphthylacetic acid; the adventitious bud rooting culture medium is composed of the YS culture medium and hormone A, wherein the hormone A consists of one or several of indolebutyric acid, indoleacetic acid and naphthylacetic acid in proportion. The populus alba * pobulus davidiana dode 1333 tissue culture medium disclosed by the invention is applied to the plant tissue culture filed.
Owner:FORESTRY RES INST OF HEILONGJIANG PROVINCE
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