9 results about "Kinetin" patented technology

The invention discloses a method for increasing the content of genistin and genistein in flemingia philippinensis by using potassium. Culturing the flemingia philippinensis tissue culture seedlings in a propagation culture medium, and subculturing to obtain flemingia philippinensis stem segments with leaves; taking stems of the flemingia philippinensis, and culturing the stems in a rooting culture medium to obtain the flemingia philippinensis with the content of genistin and genistein increased; wherein the propagation culture medium is prepared from MS (Murashige and Skoog), 6-benzyladenine, indoleacetic acid and kinetin; the rooting culture medium comprises KNO3, MS (Murashige and Skoog) and naphthylacetic acid, and the MS does not contain KNO3. According to the method for increasing the content of genistin and genistein in the flemingia philippinensis by using potassium, accumulation of active ingredients of genistin and genistein at the root of the flemingia philippinensis is adjusted by adding exogenous potassium ions, so that the utilization rate of the flemingia philippinensis is increased, and the problem of resource shortage of the flemingia philippinensis is relieved.

The application provides a method for promoting the absorption of calcium elements by pineapple fruits, which comprises the following steps: three months after the pineapple plant is induced to bloom and four months after the pineapple plant is induced to bloom, gauze is soaked in an amino oxyacetic acid aqueous solution or a pyrazine amide aqueous solution, the soaked gauze is applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours; the gauze is secondly soaked in an indole-3-acetic acid aqueous solution, an indole-3-butyric acid aqueous solution, a phenylacetic acid aqueous solution or an alpha-naphthylacetic acid aqueous solution, and the soaked gauze is again applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours; the gauze is thirdly soaked in a 6-benzylaminopurine aqueous solution, a kinetin aqueous solution, a thidiazuron aqueous solution or a forchlorfenuron aqueous solution, and the soaked gauze is again applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours. The application can inhibit the formation of the pineapple fruit stem delimitation, promote the absorption of calcium by the fruits, reduce the incidence of pineapple water core disease from about 18% to about 0.6%, and significantly improve the income of fruit farmers.

The invention discloses a rapid propagation rooting culture medium for peony seed tissue culture and a culture method, and belongs to the technical field of plant tissue culture. The rapid propagation rooting culture medium takes 1 / 2 MS as a basic culture medium, and further comprises the following components: ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, monopotassium phosphate, a carbon source, kinetin and a curing agent. By adding kinetin into the culture medium, dependence of peony seed tissue culture seedlings on low-temperature treatment is broken, low-temperature pretreatment necessary in a traditional method is avoided, the peony seed tissue culture process is greatly simplified, the culture period is shortened, the rooting rate and the root strengthening rate are increased, the rooting rate is stabilized to be 95% or above, and the rooting rate is stabilized to be more than 95%. Technical support is provided for establishment of a new peony seed tissue culture and rapid propagation technology, and a material foundation is laid for promotion of peony seed tissue culture and rapid propagation factorization.

The present application belongs to the field of plant tissue culture and genetic transformation, and particularly relates to a method for establishing a regeneration system of green moss seeds and a genetic transformation method. The subculture medium of the method for establishing the regeneration system of the green moss seeds contains proline and kinetin. The resistant subculture medium of the genetic transformation method of the green moss seeds contains proline and kinetin, and the first and second resistant screening media contain proline and vitamin C. The genetic transformation method of the green moss seeds comprises the steps of cold treatment, vacuum treatment and dry treatment of callus. The subculture medium of the regeneration system of the green moss seeds is optimized, the regeneration efficiency is high, and a large number of regenerated plants can be obtained; the green moss genetic transformation system of the present application can obviously distinguish between the successfully transformed and unsuccessfully transformed experimental materials through twice resistant screening, and the identification accuracy is high.

The present application relates to the technical field of new antiviral drug active ingredients, and particularly relates to application of a small-molecule compound inhibitor of lactate dehydrogenase A in preparation of an antiviral drug, wherein the inhibitor is Kinetin or L67, which can be directly combined with lactate dehydrogenase A (LDHA), on one hand, significantly reduces the expression level of LDHA, reduces the negative regulation of LDHA to the IFN-I channel from the source; on the other hand, can specifically destroy the LDHA / STAT2 / HDAC3 ternary complex induced by IFN-I, antagonize the transcriptional regulation function of LDHA, and release the inhibition of the antiviral effect of IFN-I. The present application can solve the core pain point that the response rate of IFN-alpha in the treatment of chronic hepatitis B (CHB), influenza virus or new coronavirus and other viral infection diseases is not high in clinic; through the combined scheme of Kinetin / L67+IFN-I, the response efficiency of clinical interferon treatment is improved.

The invention discloses a method for increasing the content of genistein in flemingia philippinensis by using nitrogen. Culturing the flemingia philippinensis tissue culture seedlings in a propagation culture medium, and subculturing to obtain flemingia philippinensis stem segments with leaves; taking stems of the flemingia philippinensis, and culturing the stems in a rooting culture medium to obtain the flemingia philippinensis with increased genistein content; wherein the propagation culture medium is prepared from MS (Murashige and Skoog), 6-benzyladenine, indoleacetic acid and kinetin; the rooting medium comprises NH4NO3, MS (Murashige and Skoog) and naphthylacetic acid, and the MS does not contain NH4NO3. According to the method for increasing the content of genistein in the flemingia philippinensis by using nitrogen, accumulation of the active ingredient genistein at the root of the flemingia philippinensis is adjusted by adding exogenous nitrogen ions, so that the utilization rate of the flemingia philippinensis is increased, and the problem of resource shortage of the flemingia philippinensis is relieved.

The present application belongs to the technical field of plant cell culture, and relates to a method for inducing callus of potato 'Kexin 13'. The method comprises the following steps: (1) taking potato 'Kexin 13' explants and transferring them to callus induction medium to form callus, wherein the induction medium is based on MS medium and supplemented with 6-benzylaminopurine, kinetin and 2,4-dichlorophenoxyacetic acid, and has a pH of 5.8-6; (2) taking the callus formed in step (1) and transferring it to differentiation medium to form adventitious buds, wherein the differentiation medium is based on MS basic medium and contains 6-benzylaminopurine, kinetin, gibberellin and myo-inositol, and has a pH of 5.8-6. Through induction culture and differentiation culture, the callus of potato 'Kexin 13' has a high induction rate and differentiation rate.

Disclosed is a method for inducing field-planted Ananas comosus to rapidly propagate seedlings, which adopts a new technical management method for practical production of the field-planted Ananas comosus, and comprises: pruning and shaping mother plants of Ananas comosus, performing sterilization and pest prevention on the mother plants of Ananas comosus, promoting germination, performing fertilization, and propagating and culturing strong seedlings, especially implementing application management of plant kinetin, so that a seedling propagation period of the field-planted Ananas comosus is shortened by more than 60 days compared with a natural propagation period, and by means of 2-3 rounds of propagation and harvesting, a propagation coefficient of a single mother plant is improved to reach 12. The method has a high propagation rate, realizes rapid production of a large number of native offset seedlings of Ananas comosus, and greatly improves the production efficiency of Ananas comosus, and reduces a seedling propagation cost.

The invention belongs to the technical field of medicines, and particularly relates to application of kinetin in preparation of medicines for preventing and / or treating obesity, fatty liver and liver injury induced by high fat. The kinetin has a potential anti-obesity effect. Results of embodiments show that the kinetin can significantly reduce weight gain of mice caused by high fat induction, and after the kinetin is administered, the body type and white fat of the mice are reduced, the glucose tolerance is increased, the insulin sensitivity is increased, and the UCP1 (uncoupling protein 1) level of brown fat of a kinetin administration group is increased, which indicates that the kinetin can play a role in resisting obesity.