23 results about "Kinetin" patented technology

The invention discloses a method for increasing the content of genistin and genistein in flemingia philippinensis by using potassium. Culturing the flemingia philippinensis tissue culture seedlings in a propagation culture medium, and subculturing to obtain flemingia philippinensis stem segments with leaves; taking stems of the flemingia philippinensis, and culturing the stems in a rooting culture medium to obtain the flemingia philippinensis with the content of genistin and genistein increased; wherein the propagation culture medium is prepared from MS (Murashige and Skoog), 6-benzyladenine, indoleacetic acid and kinetin; the rooting culture medium comprises KNO3, MS (Murashige and Skoog) and naphthylacetic acid, and the MS does not contain KNO3. According to the method for increasing the content of genistin and genistein in the flemingia philippinensis by using potassium, accumulation of active ingredients of genistin and genistein at the root of the flemingia philippinensis is adjusted by adding exogenous potassium ions, so that the utilization rate of the flemingia philippinensis is increased, and the problem of resource shortage of the flemingia philippinensis is relieved.

The application provides a method for promoting the absorption of calcium elements by pineapple fruits, which comprises the following steps: three months after the pineapple plant is induced to bloom and four months after the pineapple plant is induced to bloom, gauze is soaked in an amino oxyacetic acid aqueous solution or a pyrazine amide aqueous solution, the soaked gauze is applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours; the gauze is secondly soaked in an indole-3-acetic acid aqueous solution, an indole-3-butyric acid aqueous solution, a phenylacetic acid aqueous solution or an alpha-naphthylacetic acid aqueous solution, and the soaked gauze is again applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours; the gauze is thirdly soaked in a 6-benzylaminopurine aqueous solution, a kinetin aqueous solution, a thidiazuron aqueous solution or a forchlorfenuron aqueous solution, and the soaked gauze is again applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours. The application can inhibit the formation of the pineapple fruit stem delimitation, promote the absorption of calcium by the fruits, reduce the incidence of pineapple water core disease from about 18% to about 0.6%, and significantly improve the income of fruit farmers.

The present invention discloses a method for sterile germination and seedling raising of the rhizome of polygonatum sibiricum redoute, a medicinal plant from the Maoshan area. The specific steps are as follows: collect immature fruits of polygonatum sibiricum redoute, soak the fruits with a medicament first to make the pulp of the berries rot, rub and wash out the seeds of polygonatum sibiricum redoute, then put them into a mixed solution of kinetin and gibberellin and mix them with river sand for low-temperature sand storage. The seeds obtained after the sand storage treatment are sterilized and disinfected. After cutting off part of the pericarp and endosperm to expose the embryo, they are put into a cluster bud induction medium for liquid oscillation culture to induce cluster buds. Finally, the cluster buds are transferred to a strong seedling and rooting medium for strong seedling and rooting culture to form tissue culture seedlings of polygonatum sibiricum redoute. The method provided by the present invention uses immature embryos of polygonatum sibiricum redoute for medicament treatment, avoiding the dormancy stage after the seeds of polygonatum sibiricum redoute mature, and increasing the seed germination rate to more than 90%. And through the two-step method of liquid oscillation culture and rooting culture of the embryos of polygonatum sibiricum redoute, the cycle of rapid propagation and seedling raising of the seeds of polygonatum sibiricum redoute is greatly shortened.

The invention discloses a rapid propagation rooting culture medium for peony seed tissue culture and a culture method, and belongs to the technical field of plant tissue culture. The rapid propagation rooting culture medium takes 1 / 2 MS as a basic culture medium, and further comprises the following components: ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, monopotassium phosphate, a carbon source, kinetin and a curing agent. By adding kinetin into the culture medium, dependence of peony seed tissue culture seedlings on low-temperature treatment is broken, low-temperature pretreatment necessary in a traditional method is avoided, the peony seed tissue culture process is greatly simplified, the culture period is shortened, the rooting rate and the root strengthening rate are increased, the rooting rate is stabilized to be 95% or above, and the rooting rate is stabilized to be more than 95%. Technical support is provided for establishment of a new peony seed tissue culture and rapid propagation technology, and a material foundation is laid for promotion of peony seed tissue culture and rapid propagation factorization.

The invention relates to the technical field of plant tissue culture, in particular to a medium-grain coffee embryonic callus induction method which comprises the following steps: selecting medium-grain coffee leaves as explants for surface sterilization; sequentially carrying out three culture stages, namely a dedifferentiation stage, an embryogenic induction stage and an embryogenic maintenance stage, on the sterilized explant; in the dedifferentiation stage, a first culture medium added with 2, 4-dichlorphenoxyacetic acid is adopted, and culture is performed under a first culture condition; in the embryogenic induction period, a second culture medium added with 2, 4-dichlorphenoxyacetic acid and 6-benzyladenine is adopted, and culture is performed under a second culture condition; in the embryogenic maintenance period, a third culture medium added with 1-naphthylacetic acid and kinetin is adopted, and culture is performed under a third culture condition; according to the method disclosed by the invention, the compact embryonic callus with low waterlogging degree is obtained, and the induction rate of the embryonic callus reaches 85-95% through three-stage progressive hormone regulation and control, which is obviously higher than that of the prior art by 30-50%.

The invention relates to a method for promoting centella asiatica seed germination, and belongs to the technical field of medicinal plant cultivation. Aiming at the technical problems of low germination rate and long period of centella asiatica seeds caused by seed coat obstacles and endogenous inhibitors, the method comprises the following steps: firstly, sequentially carrying out mechanical coat breaking, ultrasonic treatment and high-pressure permeation treatment on the seeds to break a physical barrier; mixing the seeds with a wet substrate, carrying out warm lamination at 18-25 DEG C for 25-35 days, then carrying out cold lamination at 2-6 DEG C for 50-70 days, and spraying a KNO3 solution in the cold lamination stage to activate physiological metabolism; after lamination, washing with running water, sequentially soaking with a thiourea solution, carrying out H2O2 treatment for chemical degradation, and carrying out biological adsorption by adopting an adsorbent containing bacillus subtilis and activated carbon; soaking the seeds into a mixed solution containing brassinolide, kinetin and fluridone, and carrying out gradient heating and drying; and finally carrying out germination culture. The method can significantly improve the germination efficiency and uniformity, and is suitable for centella asiatica large-scale seedling raising and medicinal resource development.

The present application belongs to the field of plant tissue culture and genetic transformation, and particularly relates to a method for establishing a regeneration system of green moss seeds and a genetic transformation method. The subculture medium of the method for establishing the regeneration system of the green moss seeds contains proline and kinetin. The resistant subculture medium of the genetic transformation method of the green moss seeds contains proline and kinetin, and the first and second resistant screening media contain proline and vitamin C. The genetic transformation method of the green moss seeds comprises the steps of cold treatment, vacuum treatment and dry treatment of callus. The subculture medium of the regeneration system of the green moss seeds is optimized, the regeneration efficiency is high, and a large number of regenerated plants can be obtained; the green moss genetic transformation system of the present application can obviously distinguish between the successfully transformed and unsuccessfully transformed experimental materials through twice resistant screening, and the identification accuracy is high.

The invention provides a rapid seedling raising method for pseudo-ginseng tissue culture, which comprises the following steps: (1) direct induction of explants: selecting leaves of pseudo-ginseng tissue culture seedlings or field seedlings as explants, tearing off petioles, lightly pressing wounds to enable the cuts to be in contact with the surface of a culture medium, carrying out culture by adopting a direct induction culture medium, skipping a callus stage, and taking the explants as explants; directly generating bud points; (2) anti-browning and hormone dynamic regulation and control: adding an explant incision protective agent into the direct induction culture medium, wherein the explant incision protective agent comprises activated carbon, polyvinylpyrrolidone and a silicon element concentrated solution; regulating and controlling the hormone proportion in stages; in the primary induction stage, adding salicylic acid and naphthylacetic acid; in the secondary induction stage, single naphthylacetic acid is adjusted; in a rooting stage, indolebutyric acid and kinetin are combined. The method mainly solves the problems of long period and high browning rate in panax notoginseng tissue culture.

The present invention discloses a promoting healing solution for grafted seedlings of cucurbit vegetables, which includes liquid A and liquid B. Liquid A contains paclobutrazol and potassium dihydrogen phosphate, and liquid B contains indole-3-acetic acid, kinetin, glucose and compound amino acids. The promoting healing solution for grafted seedlings can improve the matching of rootstock / scion of cucurbit vegetables, promote the connection of phloem and xylem of grafted cucurbit seedlings, achieve the rapid healing of grafted cucurbit seedlings under low night temperature conditions, shorten the healing time, and reduce the heating cost during the healing period.

The present application relates to the technical field of new antiviral drug active ingredients, and particularly relates to application of a small-molecule compound inhibitor of lactate dehydrogenase A in preparation of an antiviral drug, wherein the inhibitor is Kinetin or L67, which can be directly combined with lactate dehydrogenase A (LDHA), on one hand, significantly reduces the expression level of LDHA, reduces the negative regulation of LDHA to the IFN-I channel from the source; on the other hand, can specifically destroy the LDHA / STAT2 / HDAC3 ternary complex induced by IFN-I, antagonize the transcriptional regulation function of LDHA, and release the inhibition of the antiviral effect of IFN-I. The present application can solve the core pain point that the response rate of IFN-alpha in the treatment of chronic hepatitis B (CHB), influenza virus or new coronavirus and other viral infection diseases is not high in clinic; through the combined scheme of Kinetin / L67+IFN-I, the response efficiency of clinical interferon treatment is improved.

The invention discloses a method for increasing the content of genistein in flemingia philippinensis by using nitrogen. Culturing the flemingia philippinensis tissue culture seedlings in a propagation culture medium, and subculturing to obtain flemingia philippinensis stem segments with leaves; taking stems of the flemingia philippinensis, and culturing the stems in a rooting culture medium to obtain the flemingia philippinensis with increased genistein content; wherein the propagation culture medium is prepared from MS (Murashige and Skoog), 6-benzyladenine, indoleacetic acid and kinetin; the rooting medium comprises NH4NO3, MS (Murashige and Skoog) and naphthylacetic acid, and the MS does not contain NH4NO3. According to the method for increasing the content of genistein in the flemingia philippinensis by using nitrogen, accumulation of the active ingredient genistein at the root of the flemingia philippinensis is adjusted by adding exogenous nitrogen ions, so that the utilization rate of the flemingia philippinensis is increased, and the problem of resource shortage of the flemingia philippinensis is relieved.

The invention provides a plant biological bacterium nutrient solution formula and a preparation process thereof, and relates to the technical field of plant biological bacterium culture, the nutrient solution comprises major elements, trace elements, organic components, a biological bacterium agent, a plant growth regulator and a medium element supplement; the organic components comprise cane sugar, amino acid, humic acid, a yeast extract, citric acid and malic acid; the biological agent is prepared from bacillus subtilis, bacillus mucilaginosus and bacillus megatherium; the plant growth regulator comprises indoleacetic acid, kinetin and gibberellin; the medium element supplement comprises calcium chloride and calcium sulfate. The plant biological bacterium nutrient solution has the advantages that by adding the nutritional ingredients, the nutritional system of the plant biological bacterium nutrient solution is further enriched, more comprehensive nutritional support is provided for growth of plants, meanwhile, plant growth regulators are contained in the nutritional ingredients, the plant growth regulators can directly promote the plants, and therefore the plants can grow rapidly.

The invention relates to the technical field of plant tissue culture, in particular to a medium-grain coffee embryonic callus induction method which comprises the following steps: selecting medium-grain coffee leaves as explants for surface sterilization; sequentially carrying out three culture stages, namely a dedifferentiation stage, an embryogenic induction stage and an embryogenic maintenance stage, on the sterilized explant; in the dedifferentiation stage, a first culture medium added with 2, 4-dichlorphenoxyacetic acid is adopted, and culture is performed under a first culture condition; in the embryogenic induction period, a second culture medium added with 2, 4-dichlorphenoxyacetic acid and 6-benzyladenine is adopted, and culture is performed under a second culture condition; in the embryogenic maintenance period, a third culture medium added with 1-naphthylacetic acid and kinetin is adopted, and culture is performed under a third culture condition; according to the method disclosed by the invention, the compact embryonic callus with low waterlogging degree is obtained, and the induction rate of the embryonic callus reaches 85-95% through three-stage progressive hormone regulation and control, which is obviously higher than that of the prior art by 30-50%.

The present application belongs to the technical field of plant cell culture, and relates to a method for inducing callus of potato 'Kexin 13'. The method comprises the following steps: (1) taking potato 'Kexin 13' explants and transferring them to callus induction medium to form callus, wherein the induction medium is based on MS medium and supplemented with 6-benzylaminopurine, kinetin and 2,4-dichlorophenoxyacetic acid, and has a pH of 5.8-6; (2) taking the callus formed in step (1) and transferring it to differentiation medium to form adventitious buds, wherein the differentiation medium is based on MS basic medium and contains 6-benzylaminopurine, kinetin, gibberellin and myo-inositol, and has a pH of 5.8-6. Through induction culture and differentiation culture, the callus of potato 'Kexin 13' has a high induction rate and differentiation rate.

Disclosed is a method for inducing field-planted Ananas comosus to rapidly propagate seedlings, which adopts a new technical management method for practical production of the field-planted Ananas comosus, and comprises: pruning and shaping mother plants of Ananas comosus, performing sterilization and pest prevention on the mother plants of Ananas comosus, promoting germination, performing fertilization, and propagating and culturing strong seedlings, especially implementing application management of plant kinetin, so that a seedling propagation period of the field-planted Ananas comosus is shortened by more than 60 days compared with a natural propagation period, and by means of 2-3 rounds of propagation and harvesting, a propagation coefficient of a single mother plant is improved to reach 12. The method has a high propagation rate, realizes rapid production of a large number of native offset seedlings of Ananas comosus, and greatly improves the production efficiency of Ananas comosus, and reduces a seedling propagation cost.

Disclosed is a method for inducing field-planted Ananas comosus to rapidly propagate seedlings, which adopts a new technical management method for practical production of the field-planted Ananas comosus, and comprises: pruning and shaping mother plants of Ananas comosus, performing sterilization and pest prevention on the mother plants of Ananas comosus, promoting germination, performing fertilization, and propagating and culturing strong seedlings, especially implementing application management of plant kinetin, so that a seedling propagation period of the field-planted Ananas comosus is shortened by more than 60 days compared with a natural propagation period, and by means of 2-3 rounds of propagation and harvesting, a propagation coefficient of a single mother plant is improved to reach 12. The method has a high propagation rate, realizes rapid production of a large number of native offset seedlings of Ananas comosus, and greatly improves the production efficiency of Ananas comosus, and reduces a seedling propagation cost.

The invention belongs to the technical field of horticulture, and particularly relates to a koelreuteria paniculata grafting method which comprises the following steps: grafting by using a cambium docking method by taking a koelreuteria paniculata seedling which is more than two years old as a stock and a koelreuteria paniculata stock crown branch as a scion; wherein before grafting, 2mL-3mL of a grafting protective agent No.1 is sprayed at the grafting part of the rootstock; after grafting, 1.5 mL-2. 5mL of grafting healing agent No.2 is sprayed on the grafting part of the scion; the first grafting protective agent is prepared from Cinnamomum camphora, cordate houttuynia, water and ethanol, the second grafting healing agent is prepared from a chitosan solution, an auxin solution, a kinetin solution, Cinnamomum camphora essential oil and Tween 80, and a mixed root system derived from a stock and a scion is formed, so that the defect of grafting incompatibility is fundamentally eliminated.

The present application discloses a method for doubling the haploid plants of watermelon. The method for doubling the haploid plants of watermelon aims to solve the problems that the use of the induction doubling agent will pollute the environment and the doubling effect of the existing method for obtaining watermelon polyploids by culturing unpollinated ovaries is not obvious. The method includes: inoculating the haploid plants obtained by in vitro culture of watermelon anthers into a medium containing MS, 0.15 - 0.25 mg / L kinetin KT, 80 - 120 ml / L fresh coconut milk, 25 - 35 g / L sucrose, 6.0 - 7.0 g / L agar, and with a pH value of 5.80 - 5.90, and then placing them in an incubator with different red and blue light quality ratios for culture. The present application can greatly improve the doubling rate of watermelon haploids by using the light quality conditions of red and blue ratio in combination with the improved medium (MS medium + exogenous substances); the in vitro development of haploid plants into diploid plants by photocatalysis not only has higher culture efficiency and is more environmentally friendly, but also has simple operation and low cost.

The present invention relates to the field of Derris truncatum cultivation technology, and more particularly to a hormone treatment method for improving the rotenone content in Derris truncatum roots. The method comprises the following steps: in a 1-2 year old field-cultivated Derris truncatum cultivation process, using a kinetin solution having a mass concentration of 100-200 mg / L to irrigate the roots of the Derris truncatum plants, with an irrigation amount of 1 L / plant, irrigating the plants twice at an interval of 7 days, and then allowing the plants to grow normally. The Derris truncatum roots are harvested 30 days after irrigating the kinetin solution. The hormone treatment method for improving the rotenone content in the Derris truncatum roots provided by the present invention can significantly increase the rotenone content in the Derris truncatum roots by 9-21% at a relatively low cost.

The invention relates to the technical field of plant tissue culture, and particularly discloses a dendrobium officinale culture medium and application thereof.The dendrobium officinale culture medium is prepared from MS basic mother liquor, cane sugar, casein hydrolysate, mashed bananas, potato extract, coconut shell powder, plant hormones (6-benayl aminopurine, naphthylacetic acid and kinetin), rare earth element compounds, activated carbon and water in specific parts. The rare earth element compound is formed by mixing lanthanum nitrate, cerium nitrate and gadolinium nitrate according to the ratio of 2: 1: 3, when the rare earth element compound is added, the lanthanum nitrate, the cerium nitrate and the gadolinium nitrate are dissolved in water on a sterile operation table and filtered and sterilized through a 0.22-micron filter membrane, meanwhile, the culture medium without rare earth is sterilized through high-pressure steam, and after the culture medium is cooled to 45-50 DEG C, the solution is injected through a quantitative pipetting device and mixed to be uniform. The culture medium is accurately blended according to the unique growth habit of dendrobium officinale, nutrients are comprehensive and balanced, the requirements of all stages can be fully met, the scientific addition of rare earth elements can promote photosynthesis and accumulation of effective components, and a good substrate is provided for artificial cultivation of dendrobium officinale in cooperation with rigorous sterilization operation.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a grape stem cell separation, culture and identification method based on stem tip meristem. Aiming at the problems that the existing grape stem cell separation steps are complicated, the induction efficiency is low and a standardized identification method is lacked, the method is realized by the following steps: collecting terminal buds or immature embryos of grapes, sterilizing, and screening and separating stem tip meristem tissues by using a sucrose solution; inoculating to an induction culture medium (containing gibberellin, kinetin, indoleacetic acid and other components), carrying out dark culture at 22 DEG C for 20-30 hours, and carrying out two-stage filtration to obtain the embryonic stem cells; then carrying out enlarged culture in a liquid culture medium (containing 2, 4-dichlorphenoxyacetic acid, gibberellin, kinetin and the like) for 20 days; and finally, detecting the expression quantity of WUS and CLV3 genes through qRT-PCR (quantitative real-time polymerase chain reaction), and verifying the characteristics of the stem cells by taking EF1a as an internal reference. According to the method, the induction time is remarkably shortened, the pollution risk is reduced, a molecular level identification standard is established, the method is suitable for the field of grape germplasm resource preservation and breeding, and an efficient and universal solution is provided for a plant stem cell technology.

The invention provides a method for promoting calcium absorption of pineapple fruits, which comprises the following steps of: soaking gauze in an aminooxyacetic acid aqueous solution or a pyrazinamide aqueous solution three months after flower forcing of a pineapple plant and four months after flower forcing, and applying the soaked gauze at the joint of the pineapple fruits and fruit stems for 2-5 hours; the gauze is soaked in the indole-3-acetic acid aqueous solution or the indole-3-butyric acid aqueous solution or the phenylacetic acid aqueous solution or the alpha-naphthylacetic acid aqueous solution for the second time, and the soaked gauze is applied to the joint of the pineapple fruit and the fruit stem for 2-5 hours again; the gauze is soaked in a 6-benayl aminopurine aqueous solution or a kinetin aqueous solution or a thidiazuron aqueous solution or a forchlorfenuron aqueous solution for the third time, and the soaked gauze is applied to the joint of the pineapple fruit and the fruit stem for 2-5 hours again. The pineapple stem separation layer can be inhibited from being formed, the calcium absorption of fruits is promoted, the incidence rate of the pineapple hydroheart disease is reduced from about 18% to about 0.6%, and the income of fruit farmers is remarkably increased.

The invention belongs to the technical field of medicines, and particularly relates to application of kinetin in preparation of medicines for preventing and / or treating obesity, fatty liver and liver injury induced by high fat. The kinetin has a potential anti-obesity effect. Results of embodiments show that the kinetin can significantly reduce weight gain of mice caused by high fat induction, and after the kinetin is administered, the body type and white fat of the mice are reduced, the glucose tolerance is increased, the insulin sensitivity is increased, and the UCP1 (uncoupling protein 1) level of brown fat of a kinetin administration group is increased, which indicates that the kinetin can play a role in resisting obesity.