15 results about "Kinetin" patented technology

The invention discloses a method for increasing the content of genistin and genistein in flemingia philippinensis by using potassium. Culturing the flemingia philippinensis tissue culture seedlings in a propagation culture medium, and subculturing to obtain flemingia philippinensis stem segments with leaves; taking stems of the flemingia philippinensis, and culturing the stems in a rooting culture medium to obtain the flemingia philippinensis with the content of genistin and genistein increased; wherein the propagation culture medium is prepared from MS (Murashige and Skoog), 6-benzyladenine, indoleacetic acid and kinetin; the rooting culture medium comprises KNO3, MS (Murashige and Skoog) and naphthylacetic acid, and the MS does not contain KNO3. According to the method for increasing the content of genistin and genistein in the flemingia philippinensis by using potassium, accumulation of active ingredients of genistin and genistein at the root of the flemingia philippinensis is adjusted by adding exogenous potassium ions, so that the utilization rate of the flemingia philippinensis is increased, and the problem of resource shortage of the flemingia philippinensis is relieved.

The application provides a method for promoting the absorption of calcium elements by pineapple fruits, which comprises the following steps: three months after the pineapple plant is induced to bloom and four months after the pineapple plant is induced to bloom, gauze is soaked in an amino oxyacetic acid aqueous solution or a pyrazine amide aqueous solution, the soaked gauze is applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours; the gauze is secondly soaked in an indole-3-acetic acid aqueous solution, an indole-3-butyric acid aqueous solution, a phenylacetic acid aqueous solution or an alpha-naphthylacetic acid aqueous solution, and the soaked gauze is again applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours; the gauze is thirdly soaked in a 6-benzylaminopurine aqueous solution, a kinetin aqueous solution, a thidiazuron aqueous solution or a forchlorfenuron aqueous solution, and the soaked gauze is again applied to the connection between the pineapple fruit and the fruit stem for 2-5 hours. The application can inhibit the formation of the pineapple fruit stem delimitation, promote the absorption of calcium by the fruits, reduce the incidence of pineapple water core disease from about 18% to about 0.6%, and significantly improve the income of fruit farmers.

The invention discloses a rapid propagation rooting culture medium for peony seed tissue culture and a culture method, and belongs to the technical field of plant tissue culture. The rapid propagation rooting culture medium takes 1 / 2 MS as a basic culture medium, and further comprises the following components: ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, monopotassium phosphate, a carbon source, kinetin and a curing agent. By adding kinetin into the culture medium, dependence of peony seed tissue culture seedlings on low-temperature treatment is broken, low-temperature pretreatment necessary in a traditional method is avoided, the peony seed tissue culture process is greatly simplified, the culture period is shortened, the rooting rate and the root strengthening rate are increased, the rooting rate is stabilized to be 95% or above, and the rooting rate is stabilized to be more than 95%. Technical support is provided for establishment of a new peony seed tissue culture and rapid propagation technology, and a material foundation is laid for promotion of peony seed tissue culture and rapid propagation factorization.

The invention relates to the technical field of plant tissue culture, in particular to a medium-grain coffee embryonic callus induction method which comprises the following steps: selecting medium-grain coffee leaves as explants for surface sterilization; sequentially carrying out three culture stages, namely a dedifferentiation stage, an embryogenic induction stage and an embryogenic maintenance stage, on the sterilized explant; in the dedifferentiation stage, a first culture medium added with 2, 4-dichlorphenoxyacetic acid is adopted, and culture is performed under a first culture condition; in the embryogenic induction period, a second culture medium added with 2, 4-dichlorphenoxyacetic acid and 6-benzyladenine is adopted, and culture is performed under a second culture condition; in the embryogenic maintenance period, a third culture medium added with 1-naphthylacetic acid and kinetin is adopted, and culture is performed under a third culture condition; according to the method disclosed by the invention, the compact embryonic callus with low waterlogging degree is obtained, and the induction rate of the embryonic callus reaches 85-95% through three-stage progressive hormone regulation and control, which is obviously higher than that of the prior art by 30-50%.

The invention relates to a method for promoting centella asiatica seed germination, and belongs to the technical field of medicinal plant cultivation. Aiming at the technical problems of low germination rate and long period of centella asiatica seeds caused by seed coat obstacles and endogenous inhibitors, the method comprises the following steps: firstly, sequentially carrying out mechanical coat breaking, ultrasonic treatment and high-pressure permeation treatment on the seeds to break a physical barrier; mixing the seeds with a wet substrate, carrying out warm lamination at 18-25 DEG C for 25-35 days, then carrying out cold lamination at 2-6 DEG C for 50-70 days, and spraying a KNO3 solution in the cold lamination stage to activate physiological metabolism; after lamination, washing with running water, sequentially soaking with a thiourea solution, carrying out H2O2 treatment for chemical degradation, and carrying out biological adsorption by adopting an adsorbent containing bacillus subtilis and activated carbon; soaking the seeds into a mixed solution containing brassinolide, kinetin and fluridone, and carrying out gradient heating and drying; and finally carrying out germination culture. The method can significantly improve the germination efficiency and uniformity, and is suitable for centella asiatica large-scale seedling raising and medicinal resource development.

The present application belongs to the field of plant tissue culture and genetic transformation, and particularly relates to a method for establishing a regeneration system of green moss seeds and a genetic transformation method. The subculture medium of the method for establishing the regeneration system of the green moss seeds contains proline and kinetin. The resistant subculture medium of the genetic transformation method of the green moss seeds contains proline and kinetin, and the first and second resistant screening media contain proline and vitamin C. The genetic transformation method of the green moss seeds comprises the steps of cold treatment, vacuum treatment and dry treatment of callus. The subculture medium of the regeneration system of the green moss seeds is optimized, the regeneration efficiency is high, and a large number of regenerated plants can be obtained; the green moss genetic transformation system of the present application can obviously distinguish between the successfully transformed and unsuccessfully transformed experimental materials through twice resistant screening, and the identification accuracy is high.

The invention provides a rapid seedling raising method for pseudo-ginseng tissue culture, which comprises the following steps: (1) direct induction of explants: selecting leaves of pseudo-ginseng tissue culture seedlings or field seedlings as explants, tearing off petioles, lightly pressing wounds to enable the cuts to be in contact with the surface of a culture medium, carrying out culture by adopting a direct induction culture medium, skipping a callus stage, and taking the explants as explants; directly generating bud points; (2) anti-browning and hormone dynamic regulation and control: adding an explant incision protective agent into the direct induction culture medium, wherein the explant incision protective agent comprises activated carbon, polyvinylpyrrolidone and a silicon element concentrated solution; regulating and controlling the hormone proportion in stages; in the primary induction stage, adding salicylic acid and naphthylacetic acid; in the secondary induction stage, single naphthylacetic acid is adjusted; in a rooting stage, indolebutyric acid and kinetin are combined. The method mainly solves the problems of long period and high browning rate in panax notoginseng tissue culture.

The present application relates to the technical field of new antiviral drug active ingredients, and particularly relates to application of a small-molecule compound inhibitor of lactate dehydrogenase A in preparation of an antiviral drug, wherein the inhibitor is Kinetin or L67, which can be directly combined with lactate dehydrogenase A (LDHA), on one hand, significantly reduces the expression level of LDHA, reduces the negative regulation of LDHA to the IFN-I channel from the source; on the other hand, can specifically destroy the LDHA / STAT2 / HDAC3 ternary complex induced by IFN-I, antagonize the transcriptional regulation function of LDHA, and release the inhibition of the antiviral effect of IFN-I. The present application can solve the core pain point that the response rate of IFN-alpha in the treatment of chronic hepatitis B (CHB), influenza virus or new coronavirus and other viral infection diseases is not high in clinic; through the combined scheme of Kinetin / L67+IFN-I, the response efficiency of clinical interferon treatment is improved.

The invention discloses a method for increasing the content of genistein in flemingia philippinensis by using nitrogen. Culturing the flemingia philippinensis tissue culture seedlings in a propagation culture medium, and subculturing to obtain flemingia philippinensis stem segments with leaves; taking stems of the flemingia philippinensis, and culturing the stems in a rooting culture medium to obtain the flemingia philippinensis with increased genistein content; wherein the propagation culture medium is prepared from MS (Murashige and Skoog), 6-benzyladenine, indoleacetic acid and kinetin; the rooting medium comprises NH4NO3, MS (Murashige and Skoog) and naphthylacetic acid, and the MS does not contain NH4NO3. According to the method for increasing the content of genistein in the flemingia philippinensis by using nitrogen, accumulation of the active ingredient genistein at the root of the flemingia philippinensis is adjusted by adding exogenous nitrogen ions, so that the utilization rate of the flemingia philippinensis is increased, and the problem of resource shortage of the flemingia philippinensis is relieved.

The present application belongs to the technical field of plant cell culture, and relates to a method for inducing callus of potato 'Kexin 13'. The method comprises the following steps: (1) taking potato 'Kexin 13' explants and transferring them to callus induction medium to form callus, wherein the induction medium is based on MS medium and supplemented with 6-benzylaminopurine, kinetin and 2,4-dichlorophenoxyacetic acid, and has a pH of 5.8-6; (2) taking the callus formed in step (1) and transferring it to differentiation medium to form adventitious buds, wherein the differentiation medium is based on MS basic medium and contains 6-benzylaminopurine, kinetin, gibberellin and myo-inositol, and has a pH of 5.8-6. Through induction culture and differentiation culture, the callus of potato 'Kexin 13' has a high induction rate and differentiation rate.

Disclosed is a method for inducing field-planted Ananas comosus to rapidly propagate seedlings, which adopts a new technical management method for practical production of the field-planted Ananas comosus, and comprises: pruning and shaping mother plants of Ananas comosus, performing sterilization and pest prevention on the mother plants of Ananas comosus, promoting germination, performing fertilization, and propagating and culturing strong seedlings, especially implementing application management of plant kinetin, so that a seedling propagation period of the field-planted Ananas comosus is shortened by more than 60 days compared with a natural propagation period, and by means of 2-3 rounds of propagation and harvesting, a propagation coefficient of a single mother plant is improved to reach 12. The method has a high propagation rate, realizes rapid production of a large number of native offset seedlings of Ananas comosus, and greatly improves the production efficiency of Ananas comosus, and reduces a seedling propagation cost.

Disclosed is a method for inducing field-planted Ananas comosus to rapidly propagate seedlings, which adopts a new technical management method for practical production of the field-planted Ananas comosus, and comprises: pruning and shaping mother plants of Ananas comosus, performing sterilization and pest prevention on the mother plants of Ananas comosus, promoting germination, performing fertilization, and propagating and culturing strong seedlings, especially implementing application management of plant kinetin, so that a seedling propagation period of the field-planted Ananas comosus is shortened by more than 60 days compared with a natural propagation period, and by means of 2-3 rounds of propagation and harvesting, a propagation coefficient of a single mother plant is improved to reach 12. The method has a high propagation rate, realizes rapid production of a large number of native offset seedlings of Ananas comosus, and greatly improves the production efficiency of Ananas comosus, and reduces a seedling propagation cost.

The invention belongs to the technical field of horticulture, and particularly relates to a koelreuteria paniculata grafting method which comprises the following steps: grafting by using a cambium docking method by taking a koelreuteria paniculata seedling which is more than two years old as a stock and a koelreuteria paniculata stock crown branch as a scion; wherein before grafting, 2mL-3mL of a grafting protective agent No.1 is sprayed at the grafting part of the rootstock; after grafting, 1.5 mL-2. 5mL of grafting healing agent No.2 is sprayed on the grafting part of the scion; the first grafting protective agent is prepared from Cinnamomum camphora, cordate houttuynia, water and ethanol, the second grafting healing agent is prepared from a chitosan solution, an auxin solution, a kinetin solution, Cinnamomum camphora essential oil and Tween 80, and a mixed root system derived from a stock and a scion is formed, so that the defect of grafting incompatibility is fundamentally eliminated.

The invention provides a method for promoting calcium absorption of pineapple fruits, which comprises the following steps of: soaking gauze in an aminooxyacetic acid aqueous solution or a pyrazinamide aqueous solution three months after flower forcing of a pineapple plant and four months after flower forcing, and applying the soaked gauze at the joint of the pineapple fruits and fruit stems for 2-5 hours; the gauze is soaked in the indole-3-acetic acid aqueous solution or the indole-3-butyric acid aqueous solution or the phenylacetic acid aqueous solution or the alpha-naphthylacetic acid aqueous solution for the second time, and the soaked gauze is applied to the joint of the pineapple fruit and the fruit stem for 2-5 hours again; the gauze is soaked in a 6-benayl aminopurine aqueous solution or a kinetin aqueous solution or a thidiazuron aqueous solution or a forchlorfenuron aqueous solution for the third time, and the soaked gauze is applied to the joint of the pineapple fruit and the fruit stem for 2-5 hours again. The pineapple stem separation layer can be inhibited from being formed, the calcium absorption of fruits is promoted, the incidence rate of the pineapple hydroheart disease is reduced from about 18% to about 0.6%, and the income of fruit farmers is remarkably increased.

The invention belongs to the technical field of medicines, and particularly relates to application of kinetin in preparation of medicines for preventing and / or treating obesity, fatty liver and liver injury induced by high fat. The kinetin has a potential anti-obesity effect. Results of embodiments show that the kinetin can significantly reduce weight gain of mice caused by high fat induction, and after the kinetin is administered, the body type and white fat of the mice are reduced, the glucose tolerance is increased, the insulin sensitivity is increased, and the UCP1 (uncoupling protein 1) level of brown fat of a kinetin administration group is increased, which indicates that the kinetin can play a role in resisting obesity.