The invention discloses a
calanthe japonica flower color and flower fragrance
component analysis method, and belongs to the technical field of
plant component analysis. The method comprises the following steps: S1, synchronous collection and pretreatment of samples: collecting flower buds and flowers of the same scapes, respectively taking petals and lips, and uniformly dividing the petals and lips into flower fragrance and
pigment detection samples; s2, targeted extraction and GC-MS (
gas chromatography-
mass spectrometry) detection of flower fragrance components: extracting by adopting headspace
solid-phase microextraction combined with an
internal standard method, and performing GC-
MS analysis by optimizing a heating program; s3, high-efficiency extraction and HPLC (
High Performance Liquid Chromatography) detection of the
anthocyanidin: carrying out ultrasonic extraction by adopting a composite
solvent containing
vitamin C, and carrying out
HPLC analysis through
gradient elution; and S4,
data processing and dynamic
correlation analysis: performing qualitative and quantitative analysis on a detection result. Through targeted sampling and composite extraction technologies, the problems of high sample consumption, long period and lack of data relevance in a traditional method are solved, accurate analysis of the flower color and flower fragrance components of the
calanthe plants is realized, and a key
technical support is provided for gardening breeding.