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Cultivating method for increasing flowering rate of unfolded phalaenopsis aphrodite Rchb. F. of cherries

A cultivation method and technology for Phalaenopsis, applied in the directions of flower cultivation, horticultural methods, botanical equipment and methods, etc., can solve the problems of unrecorded flowering, no small flowers and pink lips, etc., and achieve good bifurcation and excellent The effect of excellent flowering rate and flower type

Inactive Publication Date: 2019-04-16
芜湖东源新农村开发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the existing Phalaenopsis has numerous varieties, there are still no pulverized red lips of small flowers, the varieties with excellent flower type and good bifurcation; simultaneously in the prior art, there is no record of what kind of cultivation method can better urge The variety carries flowering

Method used

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  • Cultivating method for increasing flowering rate of unfolded phalaenopsis aphrodite Rchb. F. of cherries
  • Cultivating method for increasing flowering rate of unfolded phalaenopsis aphrodite Rchb. F. of cherries
  • Cultivating method for increasing flowering rate of unfolded phalaenopsis aphrodite Rchb. F. of cherries

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0047] 1) Cross-pollination with Phal.Jiaho Cherry as the female parent and Phal.Lobbii as the male parent to obtain hybrid seeds;

[0048] 2) Cultivate the hybrid seeds (temperature is 28°C, relative humidity is 65%, light intensity is 10000lux) and select the optimal plants;

[0049] 3) Take the bud tip of the best plant and soak it in alcohol for 25 seconds for disinfection, and then put it on the primary medium (the primary medium is MS medium + 0.2mg / L folic acid + 2.5mg / L 6-BA + 1.3mg / L Mashed potatoes + 0.3mg / L ascorbic acid) for primary culture (cultivation time is 23 days, temperature is 25°C, light time is 11h / day, light intensity is 1800Lux) to obtain callus;

[0050]4) Take the base of the small shoots on the callus and cut them into small pieces, and then place them in the subculture medium (the subculture medium is MS medium + 0.2mg / L ABA + 1.9mg / L 6-BA + 0.4mg / L penicillin + 20g / L sucrose + 6g / L agar) for subculture (cultivation time is 29 days, temperature is...

preparation example 2

[0053] 1) Cross-pollination with Phal.Jiaho Cherry as the female parent and Phal.Lobbii as the male parent to obtain hybrid seeds;

[0054] 2) Cultivate hybrid seeds (temperature is 25°C, relative humidity is 70%, light intensity is 12000lux) and select the optimal plants;

[0055] 3) Take the bud tip of the best plant and soak it in alcohol for 20 seconds for disinfection, and then put it on the primary medium (the primary medium is MS medium + 0.1mg / L folic acid + 2mg / L 6-BA + 1.2mg / L potato Mud + 0.2mg / L ascorbic acid) for primary culture (cultivation time is 20 days, temperature is 23°C, light time is 10h / day, light intensity is 1500Lux) to obtain callus;

[0056] 4) Take the base of the small shoots on the callus and cut them into small pieces, and then place them in the subculture medium (the subculture medium is MS medium + 0.1mg / L ABA + 1.5mg / L 6-BA + 0.2mg / L penicillin + 15g / L sucrose + 4g / L agar) for subculture (cultivation time is 28 days, temperature is 23°C, lig...

preparation example 3

[0059] 1) Cross-pollination with Phal.Jiaho Cherry as the female parent and Phal.Lobbii as the male parent to obtain hybrid seeds;

[0060] 2) Cultivate hybrid seeds (temperature is 30°C, relative humidity is 60%, light intensity is 8000lux) and select the optimal plants;

[0061] 3) Take the bud tip of the best plant and soak it in alcohol for 30 seconds for disinfection, and then put it on the primary medium (the primary medium is MS medium + 0.3mg / L folic acid + 3mg / L 6-BA + 1.5mg / L potato Mud + 0.4mg / L ascorbic acid) for primary culture (cultivation time is 25 days, temperature is 27°C, light time is 12h / day, light intensity is 2000Lux) to obtain callus;

[0062] 4) Take the base of the small shoots on the callus and cut them into small pieces, and then place them on the subculture medium (the subculture medium is MS medium + 0.1-0.3mg / L ABA + 2.2mg / L 6-BA + 0.6 mg / L penicillin + 25g / L sucrose + 8g / L agar) for subculture (cultivation time is 30 days, temperature is 27°C, ...

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Abstract

The invention discloses a cultivating method for increasing the flowering rate of unfolded phalaenopsis aphrodite Rchb. F. of cherries. The method comprises the steps of a, planting seedlings of phalaenopsis aphrodite Rchb. F. of the cherries under the illumination of 8,000-12,000 lux to be cultivated for 5 months to obtain small seedlings; b, putting the plantlets under the illumination of 12,000-15,000 lux to be cultivated for 1 month to obtain intermediate seedlings; c, putting the intermediate seedlings under the illumination of 15,000-18,000 lux to be cultivated for 2-3 months to obtain large seedlings; d, transferring the large seedlings into a cold chamber to conduct flower bud induction and flowering accelerating until flowering is achieved, wherein the day temperature in the coldchamber is 21-23 DEG C, and the night temperature is 17-19 DEG C. A cultivating method for unfolded phalaenopsis aphrodite Rchb. F. of the cherries comprises the following steps of 1, conducting crosspollination with phal.Jiaho cherries as female parents and phal.Lobbii as male parents to obtain hybrid seeds; 2, cultivating the hybrid seeds to select optimal plants; 3, conducting tissue cultivation on the optimal plants to obtain unfolded phalaenopsis aphrodite Rchb. F. of the cherries. By means of the cultivating method, unfolded phalaenopsis aphrodite Rchb. F. of the cherries can have the excellent flowering rate.

Description

technical field [0001] The invention relates to a cultivation method of Phalaenopsis, in particular to a cultivation method for improving the flowering rate of the spreading cherry Phalaenopsis. Background technique [0002] Phalaenopsis aphrodite Rchb. F. belongs to the genus Phalaenopsis Phalaenopsis. It is native to subtropical rainforest and is an epiphytic orchid. The thick white aerial roots of Phalaenopsis are exposed around the leaves. In addition to absorbing nutrients in the air, they also have growth and photosynthesis. In the Spring Festival, the Phalaenopsis plants have long flower stalks from the leaf axils and bloom like butterflies. They are favored by flower lovers and are known as the "Queen of Orchids". Distributed in Thailand, Philippines, Malaysia, Indonesia, and Taiwan. [0003] Although the existing Phalaenopsis has numerous varieties, there are still no pulverized red lips of small flowers, the varieties with excellent flower type and good bifurca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00A01G22/63
CPCA01H1/02A01H4/001A01H4/008A01G22/63
Inventor 汪锡文裴宝平邱许凤赖本智汤蓉
Owner 芜湖东源新农村开发股份有限公司
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