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Method for inducing clustered shoots to rapidly propagate butterfly orchid by utilizing pedicle stem segments after bud cutting

A technology of phalaenopsis and pedicel, which is applied in the field of phalaenopsis propagation, can solve the problems of low production of commercial seedlings, slow plant reproduction rate, and difficulty in germination, so as to improve the reproduction rate and production volume, facilitate factory management, and save production costs Effect

Inactive Publication Date: 2015-05-13
赤峰市农牧科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of method that utilizes the pedicel stem segment after bud cutting to induce clustered buds to rapidly propagate Phalaenopsis, solve the problem that Phalaenopsis ramets are slow, seeds are not easy to germinate under natural conditions, plant propagation rate is slow, and the production capacity of commercial seedlings is low. Low, inconsistent growth cycle, uneven flowering time, unable to control flowering period and listing in large quantities, etc.

Method used

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Examples

Experimental program
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Embodiment 1

[0024] (1) selection of explant: choose the Phalaenopsis cultivar black jack plant that does not have damage by disease and insect, treat that flower withers, flowering period has passed, with scissors the whole pedicel is cut off, cuts the long pedicel axillary bud stem segment of 3-4cm (one section one Bud) is explant material;

[0025] (2) Preparation of culture medium:

[0026] Bud induction medium: MS+6-BA2.5mg / L+NAA0.5mg / L, sucrose 20g / L, pH 5.6:

[0027] Bud proliferation medium: MS+6-BA5.0mg / L+NAA0.5mg / L, sucrose 20g / L, pH 5.6:

[0028] Rooting and strong seedling medium: 1 / 2MS+NAA1.0mg / L+banana puree 100g / L, sucrose 20g / L, pH 5.6;

[0029] (3) Disinfection treatment of explants: After rinsing the above explant materials under tap water for 30-60 minutes, put them into a 75% alcohol solution in an ultra-clean workbench for 45 seconds, then blot them dry with sterile filter paper Alcohol, sterilized in 7-10% NaClO disinfectant for 12-15min, aged material sterilized f...

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Abstract

The invention discloses a method for propagating plants rapidly. The method comprises the following steps that butterfly orchid pedicle auxiliary buds after flowering are induced to form nutritional buds, and then the nutritional buds are cut, and the abandoned pedicle stem segments act as explants, and are directly inoculated into an induction culture medium for inducing clustered buds; the novel induced buds are cut off to be inoculated to the propagation culture medium for subculture propagation culture; finally rooting and strenghening are carried out and tissue culture seedlings are transplanted. By the method, the auxiliary buds on the butterfly orchid pedicles after flowering and abandoned pedicle materials after bud cutting germinate in a manually prepared culture medium for multiple times to generate novel buds. The method has the advantages that not only is the normal flower period of butterfly orchid not affected, but also butterfly orchid mother plants are not hurt, the butterfly orchid pedicle stem segments are recycled for 2-3 times; pedicle materials are used fully, waste is turned into wealth; the propagation velocity and the production of butterfly orchid tissue culture seedlings are improved greatly; the produced seed seedlings are strong and neat, and are convenient to manage in a factory.

Description

technical field [0001] The invention belongs to the technical field of phalaenopsis propagation, in particular to a method for rapidly propagating phalaenopsis by using bud-cut pedicel segments to induce clustered buds. Background technique [0002] Phalaenopsis sp. is a Phalaenopsis plant of the family Orchidaceae. It was discovered in 1750. Most of them are produced in humid Asian regions. They are naturally distributed in Aloom, Myanmar, Indian Ocean islands, Malay Peninsula, and Nanyang Islands. , the Philippines and Taiwan, China and other low-latitude tropical islands. There are more than 70 kinds of native species, and there are countless hybrids. The changes in flower color and pattern emerge in endlessly, including white flower, red flower, pink, yellow flower, reticulate, tiger-spotted, and stippled. Its flower shape is peculiar, its posture is elegant, its color is gorgeous, and its flowering period is long. It is known as the "Queen of Orchids" and has a high or...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郝永丽高博胡海波李杰王燕春柴晓娇刘莉莉张晓荣孟令强曲宝茹巴图贺磊张金成郑伟崔聪聪廉宇赵伟强刘艳春张姼琦明玉
Owner 赤峰市农牧科学研究院
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