Method for inducing clustered shoots to rapidly propagate butterfly orchid by utilizing pedicle stem segments after bud cutting
A technology of phalaenopsis and pedicel, which is applied in the field of phalaenopsis propagation, can solve the problems of low production of commercial seedlings, slow plant reproduction rate, and difficulty in germination, so as to improve the reproduction rate and production volume, facilitate factory management, and save production costs Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0024] (1) selection of explant: choose the Phalaenopsis cultivar black jack plant that does not have damage by disease and insect, treat that flower withers, flowering period has passed, with scissors the whole pedicel is cut off, cuts the long pedicel axillary bud stem segment of 3-4cm (one section one Bud) is explant material;
[0025] (2) Preparation of culture medium:
[0026] Bud induction medium: MS+6-BA2.5mg / L+NAA0.5mg / L, sucrose 20g / L, pH 5.6:
[0027] Bud proliferation medium: MS+6-BA5.0mg / L+NAA0.5mg / L, sucrose 20g / L, pH 5.6:
[0028] Rooting and strong seedling medium: 1 / 2MS+NAA1.0mg / L+banana puree 100g / L, sucrose 20g / L, pH 5.6;
[0029] (3) Disinfection treatment of explants: After rinsing the above explant materials under tap water for 30-60 minutes, put them into a 75% alcohol solution in an ultra-clean workbench for 45 seconds, then blot them dry with sterile filter paper Alcohol, sterilized in 7-10% NaClO disinfectant for 12-15min, aged material sterilized f...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com