31 results about "Axillary shoots" patented technology

The invention discloses a melaleuca bracteata tissue culture and rapid propagation process, which comprises the following steps: S1. carrying out explant sterilization, S2. starting culture: cutting the sterilized explants to 2-3cm tender stems, and inoculating the stems in a start-up culture medium for culture; S3. carrying out primary culture: cutting off auxiliary buds and transferring the auxiliary buds to a primary culture medium for culture; S4. carrying out secondary culture: cutting off the maxillary buds and transferring the auxillary buds to a secondary culture medium for culture; and S5. transplanting hardened plantlets: directly transplanting tissue cultured melaleuca bracteata plantlets striking shoot and being robust in a greenhouse seedbed after the culture medium is washed off. The melaleuca bracteata tissue culture and rapid propagation process has the beneficial effects that using the tissue culture technology to obtain the melaleuca bracteata tissue culture plantlets, the culture medium formula is simple, the culture process flow is simple and quick, the operation is simple and convenient, the propagation coefficient is relatively high, and melaleuca bracteata aseptic plantlets can be easily obtained, and the hardened plantlets are easy to survive; and the melaleuca bracteata tissue culture and rapid propagation process is time-saving and labor-saving, and provides technical support for industrialized seedling production of melaleuca bracteata and deep processing thereof.

The invention discloses a cultivation method for tetraploid Petunia hybrida. The cultivation method comprises the following steps: mutagenizing the axillary buds of a diploid Petunia hybrida plant with a chemical inducer, and after mutagenized axillary buds germinate and grow into branches, identifying and screening out tetraploid branches; with the tetraploid Petunia hybrida with good fertility as a female parent, emasculating the tetraploid Petunia hybrida, smearing mature 2n pollen from mutagenized tetraploid branches on the stigma of the female parent, wherein F1 hybrid seeds are obtainedafter a plant bears fruit; and screening a tetraploid plant from the generation F1, and carrying out pedigree selection so as to obtain a tetraploid Petunia hybrida variety with excellent characters.The method provided by the invention can improve the mutagenesis rate of a polyploid; and a tetraploid variety cultivated by using the method provided by the invention has more extensive genetic diversities, inherits a plurality of excellent characters of a diploid male parent, and has high ornamental properties and high seed setting rate; meanwhile, the cultivation method provided by the invention has low requirements on an operation site, instruments and equipment, and is relatively simple and practicable in the process of operation.

The invention provides a method for tissue culture and fast propagation of Chongyang wood, which uses Chongyang wood with axillary bud stem segments or Chongyang wood leaves as explants for tissue culture propagation. When the explants are Chongyang wood with axillary bud stem segments, the cultivation method is: sterilize the bud stem segments, then place the bud stem segments in the induction medium to directly obtain clump seedlings, and then cut the clump seedlings into individual seedlings and inoculate them one by one. Rooting culture is carried out on the rooting medium, and finally the seedlings after rooting and culture are refined and transplanted in sequence, so as to realize the rapid propagation of Chongyang tree. When the sterile leaves of Chongyang wood are used as explants, the culture method is as follows: sterilize Chongyang wood seeds, and cultivate the sterile leaves, then use the sterile leaves as the explants, and sequentially induce callus. , Callus differentiation, rooting culture process, the establishment of Chongyang wood tissue culture rapid propagation system. The invention has the advantages of simple operation, low cultivation cost, high reproduction efficiency, little influence by natural environment, and good market and industrial application prospect.

The invention relates to a management method after walnut tree grafting, which belongs to the field of fruit tree grafting management. The plastic film at the grafted wound is removed within 25 days to 35 days after the grafted walnut tree survives, and the grafted wound is bandaged with newspaper; After grafting, when the new shoot of the scion grows to 20-50cm, cut off the top growth part of the new shoot, and after the axillary bud of the new shoot germinates to form a secondary branch, cut it off from the base, which can reduce the scion to a certain extent. The advantage of the top of the new shoot makes the new branch of the scion grow more vigorously, and at the same time reduces the branches that compete for nutrients, so that the tree vigor after grafting can be restored to strength as soon as possible, the wound of grafting can heal quickly, and the secondary compensation caused by grafting failure can be saved. Take labor and material costs, avoid the phenomenon that the new shoots of the year are easy to dry out due to the extreme low temperature in winter, and also prevent the scion from being blown off by strong winds, etc., and improve the preservation rate of walnut trees after grafting and the output of the next year.

The invention provides a method for quickly cultivating large-leaf hydrangea container seedlings, which comprises the following steps: 1) selecting healthy and disease-free large-leaf hydrangea seedlings, cutting new shoots, removing leaves, leaving some petioles, and cleaning them for later use; 2) ) On the ultra-clean workbench, the branches are soaked and disinfected with alcohol solution and mercuric solution successively and rinsed; 3) The branches are cut into small sections, inserted into the starting medium and placed in the cultivation room for cultivation; 4) After cultivating for a period of time , the axillary buds germinated, cut off and inserted into the proliferation medium for culture; 5) After cultivating for a period of time, a small amount of callus tissue was formed at the base, and adventitious buds were formed. From the callus, the plants were divided into several clusters, and clustered seedlings were inserted Cultivate in the induction medium, further induce adventitious buds and rooting, form multi-branched rooted seedlings, and place them in the greenhouse for domestication; 6) After domestication for a period of time, clean the seedlings, and harden the seedlings in a full-light spray greenhouse; 7) Harden the seedlings After completion, plant in a 1-gallon container for the next step of cultivation to form a full-bodied container seedling product. The method for quickly cultivating large-leaf hydrangea container seedlings of the present invention saves the process of pinching the heart, has a short cultivation period, does not affect the flowering of the next year, has high consistency, and has a full plant type, and is suitable for large-scale container seedlings of large-leaf hydrangea and factories. Anniversary production.