9 results about "Axillary shoots" patented technology

The invention provides a method for breeding curcuma aromatica through a liquid culture medium and application of the curcuma aromatica, and belongs to the technical field of plant culture.The method comprises the following steps that 1, curcuma aromatica seeds are taken, cleaned and then sequentially disinfected through ethyl alcohol, soaked in a sodium hypochlorite solution, soaked in sterile water and flushed, and disinfected seeds are obtained; (2) inoculating the disinfected seeds to a liquid culture medium, and performing germination culture on the sterile seeds on the liquid culture medium to obtain sterile tissue culture seedlings; and (3) cutting the sterile tissue culture seedlings into stem sections of 2-3cm, inoculating the stem sections with at least one pair of axillary buds to the liquid culture medium with the same composition in the step (2), and carrying out subculture on the tissue culture seedlings. The method provided by the invention has a good tissue culture effect on radix curcumae, can simultaneously realize excellent culture effects in four aspects of germination rate of seeds, average germination rate of tissue culture seedlings, growth height and differentiation rate, and has extremely high research value and application prospect.

The application provides a method for breeding radix curcumae in liquid medium and application thereof, and belongs to the technical field of plant culture. The method comprises the following steps: (1) taking radix curcumae seeds, washing, and then sequentially using alcohol disinfection, sodium hypochlorite solution immersion, sterile water immersion and washing to obtain disinfected seeds; (2) inoculating the disinfected seeds into liquid medium, and culturing the sterile seeds on the liquid medium to obtain sterile tissue culture seedlings; (3) cutting the sterile tissue culture seedlings into stem segments with a length of 2-3 cm, each of the stem segments having at least one pair of axillary buds, and inoculating the stem segments into the liquid medium of the same composition in step (2) to carry out tissue culture seedling subculture. The method has good tissue culture effect on radix curcumae, can simultaneously achieve excellent cultivation effects in the germination rate of seeds, the average germination rate of tissue culture seedlings, the growth height and the differentiation rate, and has high research value and application prospect.

The invention relates to the technical field of rose green tea production, and discloses a unique fermentation process of rose tender shoot green tea. The unique fermentation technology of the novel rose tender shoot green tea comprises the following steps of raw material selection and treatment, core fermentation and post-treatment, green tea tender shoots are subjected to low-temperature pulse ultrasonic cleaning and vacuum gradient freeze-drying and matched with a tea polyphenol protective agent, and loss of core active ingredients is greatly reduced; rose axillary buds are subjected to photoperiod domestication and composite enzymolysis to break cell walls, conditions are created for release and fusion of flavor substances, and the problem of component fusion is solved; by matching with staged regulation and control of a double-bacterium symbiotic bacterium agent, infectious microbes are inhibited, theanine is improved, and characteristic fragrance is generated; multispectral irradiation promotes generation of rose characteristic aroma and slows down degradation of chlorophyll, and aroma-producing yeast is matched to enrich ester aroma, so that aroma coordination of rose and green tea is realized; the bamboo charcoal coating of the ceramic pot adsorbs foreign flavor, and flavor purity is improved.

This invention discloses a method for introducing exogenous genes into Gynostemma pentaphyllum, comprising: S1: Selecting Gynostemma pentaphyllum seeds with seed coats within 3 months of harvest, disinfecting, and culturing to obtain aseptic seedlings from seedlings, or selecting stem segments with axillary buds after leaf removal, disinfecting, and culturing using a subculture proliferation medium containing cephalosporin to obtain aseptic rooted seedlings; S2: Selecting the aseptic seedlings or aseptic cuttings obtained in S1, culturing in a rooting medium containing cephalosporin to obtain aseptic rooted seedlings; S3: Transforming plasmid Pcambia1301-PMI into Agrobacterium and culturing in a medium containing kanamycin and rifampin to obtain an Agrobacterium resuspension; S4: Infecting the aseptic rooted seedlings obtained in S2 with the Agrobacterium resuspension, and performing co-culture, antibacterial culture, and screening culture to obtain transgenic adventitious shoots. This invention can successfully introduce exogenous genes into Gynostemma pentaphyllum, obtaining Gynostemma pentaphyllum positive plants.

The invention relates to the technical field of passion fruit planting, in particular to a labor-saving cultivation device and method for interplanting golden passion fruits and pumpkins, the device comprises a stand column, a climbing assembly allowing branches and tendrils of the passion fruits to climb is arranged on the stand column, and vertical cylinders are arranged on the two sides of the climbing assembly; notches are formed in the side walls of the stand column and the stand cylinder, and C-shaped cutting rings are arranged in the notches; springs are arranged on the inner bottom walls of the stand columns and the vertical cylinders, supporting assemblies which are in sliding fit with the inner side walls of the stand columns and the vertical cylinders and used for supporting the C-shaped cutting rings are arranged at the other ends of the springs, and first rope rings are arranged on the sides, close to the supporting assemblies, of the inner side walls of the C-shaped cutting rings. Through mechanical linkage of the C-shaped cutting rings and the blades, automatic topping of main vines and rapid removal of axillary buds are achieved, the manual participation degree is reduced, the labor cost is reduced, and the comprehensive production efficiency and economic benefits in the golden passion fruit and pumpkin interplanting mode are further improved.

The invention belongs to the technical field of tobacco topping and bud inhibition, and particularly relates to application of isobutene-fluorine in preparation of a tobacco bud inhibitor. Specifically, the invention provides the tobacco bud inhibition composition taking the isobutene-fluorine compound as the active ingredient, and the tobacco bud inhibition composition comprises the following components: 50%-75% of the isobutene-fluorine compound, 2%-6% of sodium lignin sulfonate, 1%-3% of a sodium naphthalene sulfonate formaldehyde polycondensate, 1%-3% of sodium alkyl benzene sulfonate, 2%-5% of glucose, 0.5%-1% of sodium bovine fatty acid, 4%-7% of ammonium sulfate and the balance of corn starch. The pesticide is applied within 24 hours after topping from the tobacco bud elongation stage to the early flowering stage, and the mode of cup spraying, spraying or smearing can be adopted. Field test results show that the isobutene fluorine has a remarkable inhibition effect on axillary bud germination of tobacco plants under a proper dosage, the bud inhibition effect is remarkably superior to butralin and equivalent to flumetralin, the lasting period is long, no phytotoxicity is caused to the tobacco plants, and no adverse influence is caused to the yield and quality of tobacco leaves. According to the invention, the technical prejudice is overcome, the new use of the ethyl butenin on tobacco is realized, and a new choice is provided for the replacement and alternation of the tobacco bud inhibitor.

The invention provides a tissue culture medium for rapid propagation of caulis sinomenii and a method for rapid propagation of caulis sinomenii, and belongs to the technical field of plant tissue culture. The invention provides a tissue culture medium for rapid propagation of caulis sinomenii. The tissue culture medium comprises an induction medium and a rooting medium, the induction culture medium takes an MS culture medium as a basic culture medium, and further comprises 1-2mg / L of 6-BA and 0.1-0.2 mg / L of NAA; the rooting culture medium takes a 1 / 2 MS culture medium as a basic culture medium, and further comprises 0.1-1g / L of LAc and 0.1-0.2 mg / L of NAA. According to the embodiment of the invention, stem segments with buds of caulis sinomenii are taken as explants, axillary buds are directly induced to differentiate by utilizing the tissue culture medium, a large number of seedlings can be obtained, mass propagation can be realized within a short time, and the propagation efficiency of caulis sinomenii is improved.

This invention relates to the field of tissue culture technology, and more particularly to a method for cultivating high-efficiency tissue culture seedlings of *Anoectochilus roxburghii* stem segments from northern Zhejiang. The proliferation medium provided by this invention is based on MS medium and further includes the following components at concentrations: 0.05-0.1 mg / L naphthaleneacetic acid (NAA) and 1.8-2.5 mg / L 6-benzyladenine (6-BA). The specific combination of NAA and 6-BA in the proliferation medium of this invention can effectively induce the formation of axillary buds and explant callus, and proliferate a large number of adventitious buds, significantly increasing the propagation coefficient. This is one of the core technical points for achieving "high-efficiency" rapid propagation. In addition, the reasonable combination of rooting medium and cultivation substrate can significantly improve the survival rate of tissue culture seedlings after acclimatization, ensuring a seamless transition from in vitro culture to field planting, which is an important link in achieving a closed loop of "high-efficiency and rapid propagation." This provides a basis for the high-efficiency and rapid propagation of *Anoectochilus roxburghii*.

The application discloses a kind of high-efficiency callus induction and differentiation seedling method of Arundo donax, including sterile seedling obtaining, callus induction, callus proliferation culture, differentiation culture, rooting culture and transplanting, wherein, sterile seedling obtaining: with Arundo donax with axillary bud shoot segment as explant, after disinfection is inoculated to sterile seedling induction culture medium, culture obtains sterile seedling.Callus induction: take the stem base of sterile seedling, inoculation is carried out to callus induction culture medium and is carried out light-proof culture, and callus is induced to generate.The callus proliferation culture: callus is cut, and is transferred to proliferation culture medium and is carried out subculture.The application effectively improves the induction efficiency and differentiation regeneration capacity of Arundo donax callus by optimizing explant selection, hormone proportion of culture medium and culture condition, solves the problems, such as low callus induction rate, poor embryonic, unstable differentiation and the like in prior art, and has the advantages of simple operation, short regeneration cycle, uniform seedling quality, suitable for scale production and the like.