19 results about "Axillary shoots" patented technology

The invention relates to the technical field of fruit industry, and particularly discloses a regulation and control method for promoting and strengthening flowers of citrus and application. The regulation and control method comprises the following steps: spraying a blossom-promoting regulatory factor A at an aging stage of citrus autumn shoots; and spraying a blossom-promoting regulatory factor B from one week before budding of the citrus in the next year to the flower bud stage. The flower-promoting regulation factor A comprises the following components in spraying concentration: 15 to 50 [mu] g / ml of S-abscisic acid, 25 to 100 [mu] g / ml of prohexadione calcium, 0.05 to 1 [mu] g / ml of chlorin iron, and the balance of an agriculturally acceptable auxiliary agent; and the flower-promoting regulatory factor B comprises the following components in spraying concentration: S-abscisic acid of 1-35 [mu] g / ml, benzylaminopurine of 10-70 [mu] g / ml, chlorin of 0.1-2 [mu] g / ml and the balance of agriculturally acceptable auxiliaries. The method can regulate tree axillary bud flowering hormone balance, effectively improve the next-year citrus flowering amount, improve the flower bud quality and flowering uniformity, reduce the proportion of deformed flowers, reduce the fruit retention difficulty, improve the citrus yield and improve the citrus quality.

The invention provides a method for breeding curcuma aromatica through a liquid culture medium and application of the curcuma aromatica, and belongs to the technical field of plant culture.The method comprises the following steps that 1, curcuma aromatica seeds are taken, cleaned and then sequentially disinfected through ethyl alcohol, soaked in a sodium hypochlorite solution, soaked in sterile water and flushed, and disinfected seeds are obtained; (2) inoculating the disinfected seeds to a liquid culture medium, and performing germination culture on the sterile seeds on the liquid culture medium to obtain sterile tissue culture seedlings; and (3) cutting the sterile tissue culture seedlings into stem sections of 2-3cm, inoculating the stem sections with at least one pair of axillary buds to the liquid culture medium with the same composition in the step (2), and carrying out subculture on the tissue culture seedlings. The method provided by the invention has a good tissue culture effect on radix curcumae, can simultaneously realize excellent culture effects in four aspects of germination rate of seeds, average germination rate of tissue culture seedlings, growth height and differentiation rate, and has extremely high research value and application prospect.

A plant medium composition and methodology of application of the medium as it pertains to the micropropagation of Cannabis and related plant species is described that yields two to four-fold increase in the number of biologically functional nodes from axillary shoots thereby allowing for exponential amplification of such plant species. The compositions comprise basal plant media supplemented with: (i) one or more cytokinins, and (ii) one or more gibberellins and / or brassinolides. This achievement in exponential shoot growth of these plant species represents a marked step forward in the Cannabis industry as it pertains to commercial micropropagation of a recreationally and medicinally important plant whose extracts are the subject of numerous clinical trials.

The invention discloses a high-fruiting-rate prinsepia utilis royle grafting propagation method. The method comprises the following steps: 1) selecting at least one-year-old prinsepia utilis royle seedlings which grow well and are free of plant diseases and insect pests, and the overground part diameter of the prinsepia utilis royle seedlings is 0.5-2 cm as stocks; 2) selecting middle and upper parts of excellent seed trees without diseases and insect pests to grow well as scions; 5) wrapping the cut of the cut stock and the scion with a grafting film, fixing the scion in the cut of the stock after wrapping to keep the stock and the scion forming layer tightly attached and fixed, exposing the axillary bud position on the scion without wrapping other positions, and setting a sunshade net after grafting is completed; and 6) when the young sprouts on the scions grow to 15-20cm, removing the grafting film, and cutting at least three parallel long-line wounds on the epidermis of the rootstock branches and the scion branches by using the tip of the grafting knife. According to the method, later-stage tedious management is not needed, more than 93% of prinsepia utilis royle seedlings bred through grafting bloom and bear fruits in one year after field planting, the character of the born seeds is basically consistent with that of seed trees, and a good foundation is laid for vegetative propagation of prinsepia utilis royle.

The invention relates to a pesticide composition containing flumetralin and butralin and application of the pesticide composition. The composition is used for inhibiting the growth of tobacco axillary buds and can effectively improve the tobacco yield and reduce the manual topping cost. The product has the advantages of high stability, simple preparation in the processing process, no dust generation in the production process, and effective increase of the utilization rate and reduction of the usage amount by mainly selecting the environment-friendly organic solvent, thereby reducing the residual amount in the environment. The composition has the advantages of low acute toxicity, acceptable dietary risk and safety to human and livestock, meets the actual requirements of current agricultural production, has the inhibition rate on tobacco axillary buds obviously higher than that of butralin and flumetralin, and through compounding, the dosage of a single tobacco plant is obviously reduced, and pesticide residues are reduced. And compared with similar products, the cost is lower, and the use cost of the pesticide by a user is reduced. Popularization and use of the method can enrich the pesticide market, and farmers have more choices.

The application provides a method for breeding radix curcumae in liquid medium and application thereof, and belongs to the technical field of plant culture. The method comprises the following steps: (1) taking radix curcumae seeds, washing, and then sequentially using alcohol disinfection, sodium hypochlorite solution immersion, sterile water immersion and washing to obtain disinfected seeds; (2) inoculating the disinfected seeds into liquid medium, and culturing the sterile seeds on the liquid medium to obtain sterile tissue culture seedlings; (3) cutting the sterile tissue culture seedlings into stem segments with a length of 2-3 cm, each of the stem segments having at least one pair of axillary buds, and inoculating the stem segments into the liquid medium of the same composition in step (2) to carry out tissue culture seedling subculture. The method has good tissue culture effect on radix curcumae, can simultaneously achieve excellent cultivation effects in the germination rate of seeds, the average germination rate of tissue culture seedlings, the growth height and the differentiation rate, and has high research value and application prospect.

The invention discloses a field cutting seedling raising method for idesia in autumn and winter, and relates to the technical field of plant breeding, the method comprises the following steps: in late October or late December, cutting current-year idesia branches as cutting slips into uniform stem segments, reserving axillary buds on each stem segment, flatly cutting an upper notch, obliquely cutting a lower notch on a single side, inserting the cutting slips into a seedbed, and cutting into seedlings; the soil humidity is maintained at 70%-80%, and normal growth is carried out under natural conditions. According to the method, factors such as different cutting periods, cutting depths, cutting stock plant parts, cutting thickness, cutting length, lower notch shapes, root growth promoting agent (variety and concentration) treatment and the like are systematically researched, a technical combination most suitable for field cutting seedling raising of idesia in autumn and winter is optimized, and a set of efficient idesia cutting asexual propagation method is established. The method not only provides a feasible industrial seedling raising technology for asexual propagation of the idesia polycarpa, but also has important practical significance for popularization of improved varieties of the idesia polycarpa and industrial quality and efficiency improvement.

The invention relates to the technical field of rose green tea production, and discloses a unique fermentation process of rose tender shoot green tea. The unique fermentation technology of the novel rose tender shoot green tea comprises the following steps of raw material selection and treatment, core fermentation and post-treatment, green tea tender shoots are subjected to low-temperature pulse ultrasonic cleaning and vacuum gradient freeze-drying and matched with a tea polyphenol protective agent, and loss of core active ingredients is greatly reduced; rose axillary buds are subjected to photoperiod domestication and composite enzymolysis to break cell walls, conditions are created for release and fusion of flavor substances, and the problem of component fusion is solved; by matching with staged regulation and control of a double-bacterium symbiotic bacterium agent, infectious microbes are inhibited, theanine is improved, and characteristic fragrance is generated; multispectral irradiation promotes generation of rose characteristic aroma and slows down degradation of chlorophyll, and aroma-producing yeast is matched to enrich ester aroma, so that aroma coordination of rose and green tea is realized; the bamboo charcoal coating of the ceramic pot adsorbs foreign flavor, and flavor purity is improved.

This invention discloses a method for introducing exogenous genes into Gynostemma pentaphyllum, comprising: S1: Selecting Gynostemma pentaphyllum seeds with seed coats within 3 months of harvest, disinfecting, and culturing to obtain aseptic seedlings from seedlings, or selecting stem segments with axillary buds after leaf removal, disinfecting, and culturing using a subculture proliferation medium containing cephalosporin to obtain aseptic rooted seedlings; S2: Selecting the aseptic seedlings or aseptic cuttings obtained in S1, culturing in a rooting medium containing cephalosporin to obtain aseptic rooted seedlings; S3: Transforming plasmid Pcambia1301-PMI into Agrobacterium and culturing in a medium containing kanamycin and rifampin to obtain an Agrobacterium resuspension; S4: Infecting the aseptic rooted seedlings obtained in S2 with the Agrobacterium resuspension, and performing co-culture, antibacterial culture, and screening culture to obtain transgenic adventitious shoots. This invention can successfully introduce exogenous genes into Gynostemma pentaphyllum, obtaining Gynostemma pentaphyllum positive plants.

The invention relates to the technical field of passion fruit planting, in particular to a labor-saving cultivation device and method for interplanting golden passion fruits and pumpkins, the device comprises a stand column, a climbing assembly allowing branches and tendrils of the passion fruits to climb is arranged on the stand column, and vertical cylinders are arranged on the two sides of the climbing assembly; notches are formed in the side walls of the stand column and the stand cylinder, and C-shaped cutting rings are arranged in the notches; springs are arranged on the inner bottom walls of the stand columns and the vertical cylinders, supporting assemblies which are in sliding fit with the inner side walls of the stand columns and the vertical cylinders and used for supporting the C-shaped cutting rings are arranged at the other ends of the springs, and first rope rings are arranged on the sides, close to the supporting assemblies, of the inner side walls of the C-shaped cutting rings. Through mechanical linkage of the C-shaped cutting rings and the blades, automatic topping of main vines and rapid removal of axillary buds are achieved, the manual participation degree is reduced, the labor cost is reduced, and the comprehensive production efficiency and economic benefits in the golden passion fruit and pumpkin interplanting mode are further improved.

The invention belongs to the technical field of plant culture, and particularly relates to a tissue optimization culture method of quercus acutissima. The method comprises the following steps that S1, after german oak seeds with seed coats removed are disinfected, the german oak seeds are inoculated into a germination culture medium, germination seedlings are obtained through culture, and the formula of the germination culture medium comprises an improved WPM culture medium, 6-BA and NAA; s2, the germinated seedlings are cut into stem segments with axillary buds, the stem segments are inoculated into a rapid propagation culture medium to be subjected to subculture multiplication culture, tissue culture seedlings are obtained through culture, and the rapid propagation culture medium is prepared from an improved WPM culture medium, 6-BA, NAA, calcium nitrate, cane sugar and agar; s3, transferring the tissue culture seedlings obtained by the subculture multiplication culture into a rooting culture medium for rooting culture to obtain quercus acutissima tissue culture seedlings; the formula of the rooting culture medium comprises an improved 1 / 2WPM culture medium, NAA, IBA, calcium nitrate, sucrose and agar. According to the method, a seed disinfection method, spectrum conditions, culture medium components and contents are improved, so that the proliferation efficiency is further improved, the problems of tip drying and rooting difficulty in the proliferation culture process are reduced, the quality of quercus acutissima tissue culture seedlings is improved, and the proliferation speed is increased.

The invention relates to a grafting technology for efficiently cultivating high-quality propagule nursery stocks of Machilus pauhoi. From the middle ten days of December to the middle ten days of January of the next year, selecting a 2-year-old excellent machilus pauhoi seedling which grows robustly and is free of plant diseases and insect pests and has the ground diameter of more than 1.2 cm as a stock, and taking a current-year-old branchlet which grows normally, is free of plant diseases and insect pests, has the diameter of 0.4-0.5 cm and is lignified, sap starts to flow and the phloem is easy to peel off at the middle upper part of other excellent scion-picking seed trees as a scion; cutting the rootstock and cutting the scion, inserting the scion into the rootstock notch, and binding and fixing the rootstock notch and the scion cutting surface; after grafting is carried out for 20-25 days, when axillary buds of the scions grow to 1.8-2.2 cm, binding bands for binding the scions are removed; removing the binding band for binding the rootstock 45 days after grafting; cutting off coppice shoots on the rootstock after new twigs of the scions grow to 20-25 cm. The method has the beneficial effects that the grafting survival rate of the Machilus pauhoi can be improved, the excellent hereditary characteristics of the stock and the fruiting seed tree can be fused by utilizing the grafting technology, and the high-quality propagule nursery stock of the Machilus pauhoi can be efficiently and creatively cultivated.

The invention belongs to the technical field of tobacco topping and bud inhibition, and particularly relates to application of isobutene-fluorine in preparation of a tobacco bud inhibitor. Specifically, the invention provides the tobacco bud inhibition composition taking the isobutene-fluorine compound as the active ingredient, and the tobacco bud inhibition composition comprises the following components: 50%-75% of the isobutene-fluorine compound, 2%-6% of sodium lignin sulfonate, 1%-3% of a sodium naphthalene sulfonate formaldehyde polycondensate, 1%-3% of sodium alkyl benzene sulfonate, 2%-5% of glucose, 0.5%-1% of sodium bovine fatty acid, 4%-7% of ammonium sulfate and the balance of corn starch. The pesticide is applied within 24 hours after topping from the tobacco bud elongation stage to the early flowering stage, and the mode of cup spraying, spraying or smearing can be adopted. Field test results show that the isobutene fluorine has a remarkable inhibition effect on axillary bud germination of tobacco plants under a proper dosage, the bud inhibition effect is remarkably superior to butralin and equivalent to flumetralin, the lasting period is long, no phytotoxicity is caused to the tobacco plants, and no adverse influence is caused to the yield and quality of tobacco leaves. According to the invention, the technical prejudice is overcome, the new use of the ethyl butenin on tobacco is realized, and a new choice is provided for the replacement and alternation of the tobacco bud inhibitor.

The invention discloses a malania oleifera excellent single plant explant disinfection and induced regeneration method. The method comprises the following steps: S1, disinfecting terminal buds or / and axillary buds of malania oleifera; s2, inoculating the terminal buds or / and the axillary buds into an improved MS culture medium, and culturing under preset conditions to obtain pollution-free terminal buds or / and axillary buds; s3, the pollution-free terminal buds or / and axillary buds are / is inoculated into an induction culture medium and cultured under preset induction conditions, multiple shoots are obtained, and the induction culture medium is prepared from improved MS as a base and added with 6-BA, IPA, prochloraz, cysteine hydrochloride and L-arginine; s4, dividing the cluster buds into single plants, inoculating the single plants into a subculture medium, and culturing under preset culture conditions to obtain malania oleifera single plant seedlings. Compared with the prior art, the method has the advantages that terminal buds or / and axillary buds of malania oleifera are used as explant materials, a disinfection method with high survival rate and an improved MS culture medium with obvious plant vital signs are screened out, so that more pollution-free terminal buds or / and axillary buds which grow well are obtained, and tissue culture can be performed for a long time; and more materials are provided for malania oleifera tissue culture.

The invention provides a tissue culture medium for rapid propagation of caulis sinomenii and a method for rapid propagation of caulis sinomenii, and belongs to the technical field of plant tissue culture. The invention provides a tissue culture medium for rapid propagation of caulis sinomenii. The tissue culture medium comprises an induction medium and a rooting medium, the induction culture medium takes an MS culture medium as a basic culture medium, and further comprises 1-2mg / L of 6-BA and 0.1-0.2 mg / L of NAA; the rooting culture medium takes a 1 / 2 MS culture medium as a basic culture medium, and further comprises 0.1-1g / L of LAc and 0.1-0.2 mg / L of NAA. According to the embodiment of the invention, stem segments with buds of caulis sinomenii are taken as explants, axillary buds are directly induced to differentiate by utilizing the tissue culture medium, a large number of seedlings can be obtained, mass propagation can be realized within a short time, and the propagation efficiency of caulis sinomenii is improved.

This invention relates to the field of tissue culture technology, and more particularly to a method for cultivating high-efficiency tissue culture seedlings of *Anoectochilus roxburghii* stem segments from northern Zhejiang. The proliferation medium provided by this invention is based on MS medium and further includes the following components at concentrations: 0.05-0.1 mg / L naphthaleneacetic acid (NAA) and 1.8-2.5 mg / L 6-benzyladenine (6-BA). The specific combination of NAA and 6-BA in the proliferation medium of this invention can effectively induce the formation of axillary buds and explant callus, and proliferate a large number of adventitious buds, significantly increasing the propagation coefficient. This is one of the core technical points for achieving "high-efficiency" rapid propagation. In addition, the reasonable combination of rooting medium and cultivation substrate can significantly improve the survival rate of tissue culture seedlings after acclimatization, ensuring a seamless transition from in vitro culture to field planting, which is an important link in achieving a closed loop of "high-efficiency and rapid propagation." This provides a basis for the high-efficiency and rapid propagation of *Anoectochilus roxburghii*.

The invention relates to the technical field of plant tissue culture, and specifically to a method and application of rapid seedling raising of pincushion flower tissue culture, wherein the end apical bud or axillary bud is used as an explant, which is placed on a clean bench for disinfection after cleaning, the explant is cut into single buds and inoculated in a primary generation cluster bud induction medium for dark culture, and then cultured in light and dark alternately, the induced cluster buds are cut into single buds and inoculated in a secondary proliferation medium for dark culture, and then cultured in light and dark alternately, and the proliferated cluster buds are cut into single buds and inoculated in a rooting medium for culture. The invention is a seedling breeding method using modern biotechnology, which grows in a completely artificially controlled environment, is not affected by factors such as climate and season, can be produced all over the country and all year round, and has a short reproduction cycle (single generation 40 to 50 days), a high reproduction coefficient (single generation proliferation coefficient is above 3.0), and can produce a large number of seedlings that can be used for planting in a short period of time.

The invention relates to a kiwi fruit cutting-back method. Comprising the step of accurately cutting back branches in a specific period from the beginning of flowering to the beginning of fruit setting of a kiwi fruit tree. For reserved bearing branches close to trunks or main vines, a proper amount of axillary buds are reserved for cutting back according to the thickness of the branches; and for non-reserved fruiting branches, reserving a proper number of leaves according to the vigorous degree of the kiwi fruit variety, and then cutting back. The problems that a traditional pruning period is inaccurate, a pruning mode is single and light energy utilization is neglected are solved, reasonable distribution of kiwi fruit branches is achieved through a differential cutting-back strategy, the ventilation and light transmission conditions of tree bodies are improved, the photosynthesis efficiency is enhanced, and the uniformity and quality of fruits are improved. A contrast test verifies that the thickness of the position, 1 m away from the front end, of the non-cut-back main branch of the vigorous variety is larger than or equal to 2 cm when the main branch is pruned in winter, the thickness of the position, 1 m away from the front end, of the cut-back main branch can be controlled to be 2 cm or below, and a moderate fruit-bearing branch which is moderate in thickness and full in bud is formed.

The application discloses a kind of high-efficiency callus induction and differentiation seedling method of Arundo donax, including sterile seedling obtaining, callus induction, callus proliferation culture, differentiation culture, rooting culture and transplanting, wherein, sterile seedling obtaining: with Arundo donax with axillary bud shoot segment as explant, after disinfection is inoculated to sterile seedling induction culture medium, culture obtains sterile seedling.Callus induction: take the stem base of sterile seedling, inoculation is carried out to callus induction culture medium and is carried out light-proof culture, and callus is induced to generate.The callus proliferation culture: callus is cut, and is transferred to proliferation culture medium and is carried out subculture.The application effectively improves the induction efficiency and differentiation regeneration capacity of Arundo donax callus by optimizing explant selection, hormone proportion of culture medium and culture condition, solves the problems, such as low callus induction rate, poor embryonic, unstable differentiation and the like in prior art, and has the advantages of simple operation, short regeneration cycle, uniform seedling quality, suitable for scale production and the like.