3 results about "Axillary shoots" patented technology

This invention relates to the field of tissue culture technology, and more particularly to a method for cultivating high-efficiency tissue culture seedlings of *Anoectochilus roxburghii* stem segments from northern Zhejiang. The proliferation medium provided by this invention is based on MS medium and further includes the following components at concentrations: 0.05-0.1 mg / L naphthaleneacetic acid (NAA) and 1.8-2.5 mg / L 6-benzyladenine (6-BA). The specific combination of NAA and 6-BA in the proliferation medium of this invention can effectively induce the formation of axillary buds and explant callus, and proliferate a large number of adventitious buds, significantly increasing the propagation coefficient. This is one of the core technical points for achieving "high-efficiency" rapid propagation. In addition, the reasonable combination of rooting medium and cultivation substrate can significantly improve the survival rate of tissue culture seedlings after acclimatization, ensuring a seamless transition from in vitro culture to field planting, which is an important link in achieving a closed loop of "high-efficiency and rapid propagation." This provides a basis for the high-efficiency and rapid propagation of *Anoectochilus roxburghii*.

This invention discloses a method for introducing exogenous genes into Gynostemma pentaphyllum, comprising: S1: Selecting Gynostemma pentaphyllum seeds with seed coats within 3 months of harvest, disinfecting, and culturing to obtain aseptic seedlings from seedlings, or selecting stem segments with axillary buds after leaf removal, disinfecting, and culturing using a subculture proliferation medium containing cephalosporin to obtain aseptic rooted seedlings; S2: Selecting the aseptic seedlings or aseptic cuttings obtained in S1, culturing in a rooting medium containing cephalosporin to obtain aseptic rooted seedlings; S3: Transforming plasmid Pcambia1301-PMI into Agrobacterium and culturing in a medium containing kanamycin and rifampin to obtain an Agrobacterium resuspension; S4: Infecting the aseptic rooted seedlings obtained in S2 with the Agrobacterium resuspension, and performing co-culture, antibacterial culture, and screening culture to obtain transgenic adventitious shoots. This invention can successfully introduce exogenous genes into Gynostemma pentaphyllum, obtaining Gynostemma pentaphyllum positive plants.

The application discloses a kind of high-efficiency callus induction and differentiation seedling method of Arundo donax, including sterile seedling obtaining, callus induction, callus proliferation culture, differentiation culture, rooting culture and transplanting, wherein, sterile seedling obtaining: with Arundo donax with axillary bud shoot segment as explant, after disinfection is inoculated to sterile seedling induction culture medium, culture obtains sterile seedling.Callus induction: take the stem base of sterile seedling, inoculation is carried out to callus induction culture medium and is carried out light-proof culture, and callus is induced to generate.The callus proliferation culture: callus is cut, and is transferred to proliferation culture medium and is carried out subculture.The application effectively improves the induction efficiency and differentiation regeneration capacity of Arundo donax callus by optimizing explant selection, hormone proportion of culture medium and culture condition, solves the problems, such as low callus induction rate, poor embryonic, unstable differentiation and the like in prior art, and has the advantages of simple operation, short regeneration cycle, uniform seedling quality, suitable for scale production and the like.