31 results about "Axillary shoots" patented technology

The invention discloses a method of the high-quality and high-yield cultivation of radix puerariae. The method includes the steps of 1) the selection of the land: a land with thick soil layer and the pH value of 6 to 8 is selected; 2) transplanting: the selected land is treated with weeding, deep tillage and base fertilizer applying; the seedlings of radix puerariae are planted in an inclined manner based on the angle of 30 degrees to 40 degrees with the ground according to a certain row spacing and line spacing and root fixing water is watered after planting; 3) field management: (1) rack erecting: a rack is erected when the seedling of radix puerariae grows to 20cm to 30cm to be wound by the vine of radices puerarire; (2) pruning: the pruning is carried out when the vine of radix puerariae is two meters high and lateral bine below one meter and axillary shoots are picked; (3) exposure in the sun: when the root tuber grows to be as large as a middle finger or a thumb, the soil beside the root of radix puerariae is dug to cause 1cm to 3cm of the head of the root tuber to be exposed. The invention makes improvement to the existing cultivation method and the yield of the radix puerariae is remarkably improved up to 10,000 jin per Mu (one Mu is approximately equal to 666.66 square meter) and meanwhile, the content of arrow root can reach as much as 25 percent to 30 percent generally or even exceeds 30 percent.

The invention relates to a plant tissue culture method, in particular to a tissue culture method of toxicodendron vernicifluum, and particularly relates to a high-efficiency and rapid micropropagation method for toxicodendron vernicifluum. The high-efficiency and rapid micropropagation method for toxicodendron vernicifluum comprises the following steps: (1) selecting and disinfecting an explant; (2) starting culture; (3) conducting enrichment culture; (4) conducting rooting culture; and (5) hardening seedlings and transplanting. The toxicodendron vernicifluum has the advantages of being stable in inheritable character, simple in culture process, easy to root, short in culture micropropagation period, and easy to survive after being transplanted. By adopting a method for conducting tissue culture on excellent single plants of toxicodendron vernicifluum, the propagation coefficient can achieve four to five times, the annual propagation amount of every ten effective toxicodendron vernicifluum axillary buds can still achieve more than 1.75 million calculated according to the propagation coefficient of 4.5 and the propagation period of 45 days despite pollution loss, the rapid propagation problem of the toxicodendron vernicifluum can be effectively solved, the excellent clone materials can be popularized on a large area, and foundation is laid for the industrial seedling.

The invention discloses an acer paimatum seed tissue culture method. The acer paimatum seed tissue culture method comprises the following steps: seed storage: ripe acer paimatum seeds are collected in October every year, mixed with sand, and placed and stored in a refrigerator at a temperature of 4 DEG C; seed sterilization: the stored seeds are taken out and flushed, the casings of the seeds are peeled off, and the peeled seeds are sterilized with alcohol and mercuric chloride; initiation culture: an initiation culture medium comprises 1/2MS, 30.5 mg/L of GA, 30g/L of cane sugar and 5g/L of agar, and the pH value of the initiation culture medium is 6-7; primary culture: budded seeds are inoculated to a primary culture medium for culture, the primary culture medium is provided with 1/2 MS, 30.5mg/L of GA, 0.05mg/L of NAA, 30g/L of cane sugar and 5g/L of agar, and the pH value of the primary culture medium is 6-7; subculture: after tissue culture seedlings subjected to the primary culture are cut, each section of the tissue culture seedling has 2-5 axillary buds and is transferred to a subculture medium for culture, the subculture medium is provided with 1/2MS, 0.1mg/L of TDZ, 0.05mg/L of KT, 30g/L of cane sugar and 5g/L of agar, and the pH value of the subculture medium is 6-7. The acer paimatum seed tissue culture method is simple in culture process and convenient to operate, and provides technique support for industrial acer paimatum seedling-raising.

A disclosed simple tissue culture propagation technology for acerpalmatumseiryu comprises the following steps: S1, collecting disease-free acerpalmatumseiryu branches which are born in the same year, removing excess leaves, cutting into stem segments with buds, and disinfecting; S2, inoculating the disinfected stem segments with buds to a culture medium for growth, inducing axillary buds to germinate; and S3, performing rooting culture, cutting directly induced axillalry buds, inoculating to a rooting culture medium to induce root generation. The beneficial effects of the technology comprise that: the tissue culture propagation system for seiryu is estabilished, the seiryupropagation system is broken through, and the simple tissue culture propagation technology is obtained. Tissue culture plants can be rapidly obtained without a callus stage by using the technology, and can be rapidly transplanted and survive after bred; the production cost is low, the obtained tissue culture plants have consistent heredity, then propagation coefficient is high and the propagation period is short; and the technology is an efficient approach for rapid propagation of acerpalmatumseiryu, and virus accumulation is avoided.

The invention belongs to the technical field of tea cultivation, and discloses a fast seedling culture and efficient cultivation method for camellia nitidissima. The method comprises the steps of species selection of scion wood, treatment of cutting wood, preparation of a rooting agent, preparation of nurseries, performing of a cuttage method, management of the nurseries and seedlings, and efficient cultivation. The method provided by the invention has the advantages that the utilization rate of the scion wood is high; both tender branches in the current year and old branches of 2 to 3 years can be used as breeding materials on the premise that axillary buds exist. The method has the advantages that the rooting rate is high, and the root system is developed; the rooting of the bred seedlings is mainly lenticel rooting; the rooting rate is nearly 100 percent; more than 10 roots grow on each cutting slip, and the root system is developed; the rooting period is short; and roots grow out within two weeks. The nursery management and protection method provided by the invention meets the requirements of the cuttage rooting biological characteristics of the camellia nitidissima species. Inthe method provided by the invention, sandy shale developed yellow loam needs to be selected for afforestation; the forest stand closeness is between 0.6 and 0.7, or shady slopes are selected for planting; farmyard manure is applied during daily management and protection; and rich effective substances are accumulated in leaves obtained through cultivation; and the quality is good.

The invention discloses a cultivation method for drought resistance, water retention, early growth and rapid development of tobacco seedlings. The cultivation method comprises the steps such as floating tray root protection seedling-raising, land preparation and ridging, mulch film covering, base fertilizer applying, tobacco seedling transplanting, tobacco seedling topping, and field management. The cultivation method adopts floating tray root protection seedling-raising during the tobacco seedling raising period, the survival rate of the tobacco seedlings can be effectively improved, the rootsystem of the tobacco seedlings can be protected, and the root system is ensured to be complete when the tobacco seedlings are transplanted. Land ridges are irrigated before the tobacco seedlings aretransplanted to guarantee the water content of the planting land, the soil water content is maintained through the film covering treatment, and thereby the drought resistance and water retention capacity is ensured. Before transplanting, transplanting holes are dug and a drought resistance and water retention biological fertilizer is applied, a base fertilizer is provided for the tobacco seedlings and the drought resistance and water retention capacity of the tobacco seedlings is improved, and the survival rate of the tobacco seedlings after transplanting is improved. The topping operation isperformed 8-10 days after tobacco transplanting, axillary buds are left to grow, the root system growth of the tobacco seedlings can be promoted, and the growth potential energy is accumulated.

The invention relates to a cultivation method for grapes at the position of 1.55 meters high away from the ground. The cultivation method comprises the following steps: 101, in the year of field planting of the grapes, a robust new shoot is selected to be cultivated into a trunk after germination in spring, pinching is performed on the trunk at the position of 1.55 meters high away from the ground, two robustly growing axillary shoots are selected when the sprouting axillary shoots at the top grow to 35cm or above, and pinching is performed on the axillary shoots; the axillary shoots are divided in two groups and tied in the opposite direction in a first drawstring, two main vines are formed on both sides, and pinching is performed when the length of the main vines is 70cm, so that twigs grow to maturity and survive in winter; the main vines are sheared at the position with a diameter of 0.85cm when being pruned in winter, and the rest of the axillary shoots are cut off from base portions; 102, new shoots are selected from each of the main vines near the trunk in the next spring, and inflorescences are picked off as fruiting mother twigs to be cultured next year. By means of the method, workers only need to fix the twigs and tie spikes according to the number requirements, the twigs are tied and the shoots are cut off according to the specified distance, without the need for skilled workers to operate; fruit trees after being pruned have obvious fruiting zones, nutrition zones and ventilation zones.

The invention discloses an ecological and production function collaborative improvement method suitable for stony desertification treatment, which comprises the following steps: 1) strong seedling cultivation: (1) soil selection: removing ground weeds without land leveling; (2) planting time: planting in April to May with mild climate and abundant rainfall; (3) collecting cutting slips: selecting matured and robust paper mulberry without plant diseases and insect pests as a parent body; (4) the cutting method: vertically putting the scions into the pits, enabling oblique cuts to cling to soil in the pits, and enabling the cutting depth not to bury axillary buds; and (5) covering the cuttings with soil; 2) tending management: (1) fertilizing; (2) performing moss transplanting; (3) forest land management: clearing and removing scattered dry branches and fallen leaves in the paper mulberry forest land; (4) supplementing moss; and (5) winter management: cutting off from the trunk of the paper mulberry before winter each year to promote branch germination in the next year; and 3) picking branches and leaves. The method is easy to implement, the treatment process is simple, rocky desertification adaptive restoration with ecological and production values is achieved, soil tillage disturbance is reduced, tending management is optimized, and the yield of paper mulberry trees and the quality of branches and leaves are improved.

The invention provides a method for improving space utilization rate and seedling quality of young grapes, which comprises the following steps: in the first year, after the grapes sprout in the first year, reserving a robust new shoot at the top end, arranging three iron wires, vertically binding the new shoot on the first iron wire after the new shoot grows to 50cm, removing all growing points below the first iron wire, and reserving 1-2 auxiliary shoots at the top end of the new shoot; when the auxiliary shoots grow to 10 cm, the auxiliary shoots are horizontally bound along the first iron wire, and pinching is conducted after the auxiliary shoots grow to 60 cm; when young sprouts grow to 120 cm, the young sprouts are horizontally bound along the second iron wire, and when the young sprouts grow to 150 cm, pinching is conducted. In the second year, the main vines are horizontally bound at the first iron wire and the second iron wire, and the base parts of the main vines and the ground form included angles of 70-80 degrees; removing one iron wire and all growing points of the longitudinal main vines between the first iron wire and the second iron wire, reserving selected shoots on the transverse grape main vines of the first iron wire and the second iron wire, and pinching and removing other axillary buds according to the difference of the types of new shoots in the current year, namely bearing branches and vegetative branches. Space is utilized, the thickness of main vines is increased, and nutrition and reproductive growth are both considered.

The invention discloses a tissue culture and rapid propagation method for detoxification of black peel chewing cane, which comprises the following steps: S1, selecting and treating an explant: S11, selecting, cleaning and soaking robust stalk with buds, embedding the stalk into a peat soil frame in an artificial climate box, cutting off tender axillary buds of black peel chewing cane, soaking the tender axillary buds in an alcohol solution, then soaking the tender axillary buds in a mercury solution, and washing the tender axillary buds with sterile water and draining; S2, a culture stage: S21, taking the tender axillary buds of the black peel chewing cane, putting the tender axillary buds into a primary culture medium for culture, inoculating a proliferation culture medium with cluster buds for proliferation, and transferring the cluster buds to a rooting induction culture medium for rooting culture; and S3, seedling hardening and transplanting stage: transferring the cultured seedlings to natural light for seedling hardening, planting the treated black peel chewing cane seedlings on a loose and breathable seedbed, and dividing the seedlings into single plants when the seedling height is 13-15 cm. Therefore, an excellent single-plant tissue culture seedling production system of the black peel chewing cane is established, a relatively high seedling propagation coefficient is realized, detoxified seedlings with high consistency are obtained, and the method is used for large-scale production and is beneficial to rapid propagation of the seedlings of the non-toxic black peel chewing cane.

The invention discloses a grape planting method capable of resisting gas burning disease. The method comprises the following steps: 1, applying a base fertilizer comprising sheep manure and a calcium-magnesium fertilizer in early October, and applying a second fertilizer comprising the calcium-magnesium fertilizer, potassium alginate and a wingedtooth laggera herb-ulva mixture to the roots of the grapes in early April; 2, carrying out pinching treatment on 7-8 leaves of the first new shoots, carrying out pinching treatment on main shoots for the second time in late May, and reserving 4 leaves above inflorescence for pinching; when the main shoots are pinched for the second time, removing auxiliary shoots below the inflorescence, reserving the auxiliary shoots with two or more mature leaves above the inflorescence, otherwise, performing removing, selecting two or more leaves on the auxiliary shoots with two or more mature leaves, pinching is conducted, and removing axillary buds between the leaves; 3, 3 days before bagging, applying phosphorus and potassium fertilizers and L-menthol to roots of the grapes, and spraying an aqueous solution of glucomannan, selenium yeast and sodium alginate to leaves; 4, bagging the grapes on the 10th day after fruit setting, and removing fruit bags on the 6th day before picking. The method can effectively prevent the grapes from being burnt by gas and reduce the morbidity of the gas burning disease of the grapes.

The invention discloses a tissue culture and rapid propagation method for barthea barthei. The tissue culture and rapid propagation method for the botrychium lanceolatum comprises the steps: S1, selecting barthea barthei superior tree twigs, and cutting into small segments with buds; S2, disinfecting the stem segments, then inoculating the stem segments into a 1/2MS + 0.5 mg/L 6-BA culture medium, setting the room temperature and illumination intensity, and obtaining axillary buds after 30 days; S3, transferring single buds into a WPM + 0.5 mg/L 6-BA culture medium for multiplication culture when the axillary buds grow to 2-3 cm, and obtaining clumpy buds; S4, cutting the clumpy buds into the single buds, transferring the single buds into a 1/2MS + IBA 1.0 mg/L culture medium to be cultured for 30 days, and obtaining tissue culture rooting seedlings; and S5, transplanting the tissue culture rooting seedlings after being hardened for 10-15 days, cleaning the rooting seedlings with well-developed root systems, disinfecting the rooting seedlings with 500-fold carbendazim, and then adopting light-matrix non-woven fabric bags for transplanting. The tissue culture and rapid propagation method for the barthea barthei is high in breeding efficiency and high in survival rate, the obtainedbarthea barthei seedlings are neat and consistent, and excellent characters of stock plants are maintained.

The invention discloses a camellia oleifera planting method. The method comprises the following steps: S1, selecting a flat land or a sunny gentle slope land, applying a proper amount of base fertilizer, and adjusting the pH value of soil to enable the soil to be weakly acidic; S2, cutting slips are selected to be inserted into soil; S3, selecting healthy seedlings in the step S2, and transplanting the seedlings into the planting pits in the step S1 in late winter and early spring; S4, performing field management on the seedlings. Compared with the prior art, the camellia oleifera planting method provided by the invention is relatively simple, required nutrient substances and adaptive weakly acidic soil are provided for later seedling growth, and the survival rate is increased; annual semi-lignified twigs on excellent variety seed trees are selected, that is, red brown cuttings with terminal buds stopping growing and axillary buds begin to expand are cut into soil, and rooting and survival are easy; seedlings are subjected to field management, diseases and insect pests of the seedlings are reduced, the yield of camellia oleifera seeds is increased, and economic benefits are increased.

The invention discloses a cultivation method and application of flue-cured tobacco regenerated plants. The cultivation method of flue-cured tobacco regenerated plants comprises following steps: (1) 45 to 65 days after transplantation of flue-cured tobacco plants to the field, picking off terminal buds or axillary buds of the flue-cured tobacco plants; (2) soaking the picked terminal buds or axillary buds in root growth promoting agent solution; (3)cutting and transplanting the soaked terminal buds or axillary buds to culture substrates and performing cultivation; (4) 25 to 30 days after cutting and transplanting, performing root system checking. If the base part of the terminal buds or axillary buds send forth 8 to 10 adventitious roots, the terminal buds or axillary buds are secondarily transplanted to the field or pots. Then normal management for growth of flue-cured tobacco plants is performed to obtain flue-cured tobacco regenerated plant. According to the cultivation method, terminal buds or axillary buds of flue-cured tobacco plants are adopted and cultured; the components of substrates, light, temperature and humidity are controlled during cultivation process to guarantee the environment is almost identical to adaptive environments of flue-cured tobacco plants. By use of the cultivation method, glass seedlings, yellowing and mottled tobacco are avoided, rooting rate and survival rate are high.

The invention discloses a method for cultivating tea-oil trees, which comprises the following steps: step 1, selecting excellent tea-oil tree seeds, and selecting robust and disease-free tea-oil tree seeds during selection; step 2, land selection and preparation: selecting a slope land with red soil or sandy soil on a semi-sunny slope or sunny slope with sufficient sunlight as a seedbed, and applying a base fertilizer on the seedbed before sowing; step 3, pretreating and sowing the camellia oleifera seeds selected in the step 1; step 4, performing seedling stage management on the camellia oleifera seeds, and performing weeding and fertilization management; step 5, cultivating cutting seedlings, selecting the periphery of the middle upper part of a crown of a superior single plant which is free from plant diseases and insect pests, strong in growth and more in fruiting, and cutting current-year lignified thick, straight spring shoots, summer shoots and autumn shoots which have sound axillary buds and complete leaves as cutting slips; step 6; according to the method, the survival rate of breeding can be greatly increased, meanwhile, diseases and insect pests can be reduced, and the yield of camellia oleifera seeds is increased.

The invention relates to the technical field of plant tissue culture, and particularly discloses a tissue culture method of mytilaria laosensis. The tissue culture method of the mytilaria laosensis comprises the following steps: S1, collecting top tips of current-year coppice shoots of the mytilaria laosensis, cutting off leaves, cutting the top tips into stem segments with axillary buds, and disinfecting the stem segments to obtain sterilized explants; S2, inoculating the sterilized explants into a proliferation culture medium, controlling the culture conditions that the environment temperature is 27-29 DEG C and the humidity is 80-85%, and correspondingly using red light, blue light and green light to irradiate and culture; S3, transferring the sterilized explants into a strong seedling culture medium at the temperature of 25-27 DEG C, and cutting off proliferation buds on the sterilized explants to obtain the proliferation buds after white light irradiation culture; and S4, transferring the proliferation buds into a rooting culture medium at the temperature of 27-29 DEG C and with the humidity of 80-85%, correspondingly using the red light, the blue light and the green light for irradiation culture, and transferring rooted proliferation buds into a sunlight greenhouse for seedling hardening to obtain adult seedlings. According to the tissue culture method of the mytilaria laosensis, an asexual propagation technology is used for breeding themytilaria laosensis.

The invention discloses a tissue culture propagation method for stem segments with axillary buds of various cultivated peaches, and belongs to the field of plant rapid propagation and cell engineering. The method includes the steps that (1) explants of the stem segments with the axillary buds of the cultivated species are obtained; (2) propagation induction is carried out on the axillary buds of the stem segments with axillary buds; (3) elongation rooting induction is carried out; (4) seedling hardening and transplanting are carried out; and (5) the explants of the stem segments with the axillary buds of the cultivated peaches is a jujube nectarine or a young stem segment with the axillary buds of Jingyan. The tissue culture propagation method for the stem segments with he axillary buds of the various cultivated peaches has the following advantages and beneficial effects that (1) a working mechanism is clear, scientific and innovative; (2) in-vitro stem segments with axillary buds of two cultispecies, namely the jujube nectarine and the Jingyan, which are high in yield and high in quality are taken as explant materials, and complete tissue culture propagation seedlings are obtained by utilizing the method; and (3) by using the method, a large number of cultivated species tissue culture seedlings can be obtained in a short time, the cost of obtaining cultivated species peach seedlings is reduced to a great extent, and important technical support is provided for obtaining a large number of good varieties in the future.