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Simple tissue culture propagation technology for acerpalmatumseiryu

A technology of Acer japonica and Qinglong, which is applied in the field of plant tissue culture, can solve the problems such as that the tissue culture propagation method of Qinglong has not been reported, and achieves the effects of low production cost, simple culture process and easy operation.

Active Publication Date: 2014-01-08
SICHUAN COLORLINK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is a relatively common and precious ornamental variety in the market today, but there has never been a report on the tissue culture propagation method of Qinglong

Method used

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  • Simple tissue culture propagation technology for acerpalmatumseiryu

Examples

Experimental program
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Effect test

Embodiment 1

[0020] The simple tissue culture propagation process of Acer chinensis in chicken feet, which includes the following steps:

[0021] S1. Collect disease-free stems of the same year at noon or afternoon on a sunny day, remove excess leaves, and cut them into stems with buds. Sterilize the stems with alcohol for 30s, then sterilize with mercury chloride for 5 minutes, and then Wash 4-6 times with sterile water;

[0022] S2, inoculate the sterilized stem segment with buds on the medium for growth, such as figure 1 As shown, the medium is MS+NAA 0.3mg / L, and the inoculated culture material is placed in a culture room with an illumination intensity of 2000-3000LX, an illumination time of 16h / d, and a temperature of 24-26°C. , cultured for 15 days to induce axillary bud germination;

[0023] S3, rooting culture, cutting the directly induced axillary buds and inoculating on the rooting medium, such as figure 2 As shown, the rooting medium was 1 / 2MS+NAA0.3mg / L+IAA 0.2mg / L, and the...

Embodiment 2

[0025] The simple tissue culture propagation process of Acer chinensis in chicken feet, which includes the following steps:

[0026] S1. Collect disease-free stems of the same year at noon or afternoon on a sunny day, remove excess leaves, and cut them into stems with buds. The stems with buds are first sterilized with alcohol for 50s, then sterilized with mercury chloride for 4min, and then Wash 4-6 times with sterile water;

[0027] S2, inoculate the sterilized stem segment with buds on the medium for growth, such as figure 1 As shown, the medium is MS+NAA 0.3mg / L, and the inoculated culture material is placed in a culture room with an illumination intensity of 2000-3000LX, an illumination time of 16h / d, and a temperature of 24-26°C. , cultured for 2 weeks to induce axillary bud germination;

[0028] S3, rooting culture, cutting the directly induced axillary buds and inoculating on the rooting medium, such as figure 2 As shown, the rooting medium was 1 / 2MS+NAA0.3mg / L+IAA...

Embodiment 3

[0030] The simple tissue culture propagation process of Acer chinensis in chicken feet, which includes the following steps:

[0031] S1. Collect disease-free stems of the same year at noon or afternoon on a sunny day, remove excess leaves, and cut them into stems with buds. The stems with buds are first sterilized with alcohol for 60s, then sterilized with mercuric chloride for 3 minutes, and then Wash 4-6 times with sterile water;

[0032] S2, inoculate the sterilized stem segment with buds on the medium for growth, such as figure 1 As shown, the medium is MS+NAA 0.3mg / L, and the inoculated culture material is placed in a culture room with an illumination intensity of 2000-3000LX, an illumination time of 16h / d, and a temperature of 24-26°C. , cultured for 3 weeks to induce axillary bud germination;

[0033] S3, rooting culture, cutting the directly induced axillary buds and inoculating on the rooting medium, such as figure 2 As shown, the rooting medium was 1 / 2MS+NAA0.3mg...

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Abstract

A disclosed simple tissue culture propagation technology for acerpalmatumseiryu comprises the following steps: S1, collecting disease-free acerpalmatumseiryu branches which are born in the same year, removing excess leaves, cutting into stem segments with buds, and disinfecting; S2, inoculating the disinfected stem segments with buds to a culture medium for growth, inducing axillary buds to germinate; and S3, performing rooting culture, cutting directly induced axillalry buds, inoculating to a rooting culture medium to induce root generation. The beneficial effects of the technology comprise that: the tissue culture propagation system for seiryu is estabilished, the seiryupropagation system is broken through, and the simple tissue culture propagation technology is obtained. Tissue culture plants can be rapidly obtained without a callus stage by using the technology, and can be rapidly transplanted and survive after bred; the production cost is low, the obtained tissue culture plants have consistent heredity, then propagation coefficient is high and the propagation period is short; and the technology is an efficient approach for rapid propagation of acerpalmatumseiryu, and virus accumulation is avoided.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a simple tissue culture and propagation technique of Acer palmatum Qinglong. Background technique [0002] The genus Acer (Aceraceae) is usually referred to as maple, and it is also called "maple" or "maple" in habit. There are more than 200 kinds of maple trees in the world, distributed in Asia, Europe, North America and northern Africa. There are more than 160 species in my country, accounting for more than 75% of the world's total. They are distributed all over the country, and are mainly produced in the Yangtze River Basin and the provinces to the south. Maple trees can be used as building materials, paper-making raw materials, medicine extraction, industrial oil and nectar plants. Some maple trees are rich in tree shape, elegant in posture, rich in leaf shape and changeable in leaf color, and are known as world-famous foliage plants. Acer palmatum (Acer palma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 马建华王小辉朱晓菲吴佳川沈香兰
Owner SICHUAN COLORLINK CO LTD
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