Simple Tissue Culture Propagation Technology of Acer palmatum and Red Maple
A technology of maple red maple and chicken feet, which is applied in the field of simple tissue culture propagation technology of Acer palmatum red maple, can solve the problems that the method of tissue culture propagation of red maple has not been reported, and achieve low production cost, avoid virus accumulation, formula simple effect
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Embodiment 1
[0021] The simple tissue culture propagation technique of Acer palmatum and red maple comprises the following steps:
[0022] S1. Sterilize the explants. Collect disease-free branches of Acer palmatum and red maple in the same year at noon or afternoon on a sunny day, remove excess leaves, cut into stems with buds and sterilize them. Sterilization method, first sterilize with alcohol for 30 seconds, then sterilize with mercuric chloride for 5 minutes, and then wash with sterile water for 4 to 6 times;
[0023] S2, inoculation and propagation, inoculate the sterilized stem section with buds on the growth medium, the medium is MS+IAA0.5mg / L, and place the inoculated culture material in a light intensity of 2000-3000LX, The light time is 16h / d, and the temperature is 24-26°C in a cultivation room for 15 days to induce germination of axillary buds, such as figure 1 shown;
[0024] S3, rooting culture, cutting directly induced axillary buds, inoculated on the rooting medium, such...
Embodiment 2
[0026] The simple tissue culture propagation technique of Acer palmatum and red maple comprises the following steps:
[0027] S1. Sterilize the explants. Collect disease-free branches of Acer palmatum and red maple in the same year at noon or afternoon on a sunny day, remove excess leaves, cut into stems with buds and sterilize them. Sterilization method, first sterilize with alcohol for 50 seconds, then sterilize with mercuric chloride for 4 minutes, and then wash with sterile water for 4 to 6 times;
[0028] S2, inoculation and propagation, inoculate the sterilized stem section with buds on the growth medium, the medium is MS+IAA0.5mg / L, and place the inoculated culture material in a light intensity of 2000-3000LX, The light time is 16h / d, and the temperature is 24-26°C in a culture room for 2 weeks to induce germination of axillary buds, such as figure 1 shown;
[0029] S3, rooting culture, cutting directly induced axillary buds, inoculated on the rooting medium, such as ...
Embodiment 3
[0031] The simple tissue culture propagation technique of Acer palmatum and red maple comprises the following steps:
[0032] S1. Sterilize the explants. Collect disease-free branches of Acer palmatum and red maple in the same year at noon or afternoon on a sunny day, remove excess leaves, cut into stems with buds and sterilize them. Sterilization method, first sterilize with alcohol for 60 seconds, then sterilize with mercuric chloride for 3 minutes, and then wash with sterile water for 4 to 6 times;
[0033] S2, inoculation and propagation, inoculate the sterilized stem section with buds on the growth medium, the medium is MS+IAA0.5mg / L, and place the inoculated culture material in a light intensity of 2000-3000LX, The light time is 16h / d, and the temperature is 24-26°C in a culture room for 3 weeks to induce germination of axillary buds, such as figure 1 shown;
[0034] S3, rooting culture, cutting directly induced axillary buds, inoculated on the rooting medium, such as ...
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