37 results about "Genetic traits" patented technology

This invention provides a rice gene Bph54 for resistance to brown planthopper and its application. The rice provided by this invention... Bph54 Genes are of great significance for breeding new rice varieties resistant to brown planthoppers. This invention successfully cloned the rice Bph54 gene using map-based cloning, and performed functional annotation and identification. This gene exhibits complete dominance, and both homozygous and heterozygous genotypes confer equal resistance to brown planthoppers in rice. This characteristic is beneficial for the application of this gene in breeding. Based on this, the Bph54 gene can be introduced into brown planthopper-susceptible plant varieties through traditional breeding techniques such as hybridization and backcrossing, or through genetic engineering, to cultivate new varieties of plants resistant to brown planthoppers with stable genetic traits. This invention provides important germplasm resources for effectively reducing insect damage and achieving increased and stable crop yields.

The application belongs to the technical field of genetic engineering, and particularly relates to a gRNA targeting Bombyx mori sericin 1 gene, a method for preparing coarse silk and application. The target sequence of the gRNA is shown as SEQ ID NO:1. The method for preparing coarse silk comprises the following steps: 1) constructing a gRNA expression vector targeting the Bombyx mori BmSer1 gene, and preparing a gRNA transgenic Bombyx mori; 2) crossing the gRNA transgenic Bombyx mori and Cas9 transgenic Bombyx mori, and screening to obtain double transgenic individuals with green fluorescence in eyes and bodies; 3) screening to obtain a heterozygous mutant Bombyx mori strain with the BmSer1 gene knocked out through typing detection; and 4) obtaining coarse silk by feeding the transgenic Bombyx mori. The genetic editing technology is used to change the genetic traits of Bombyx mori, and the obtained silk has a larger diameter, a lower sericin rate, better water resistance and durability.

The application discloses a Cmfsg gene mutant of melon and a creation method and application thereof, and belongs to the technical field of plant genetic engineering. The mutant is obtained by CRISPR / Cas9 gene editing technology to direct mutation of the coding region or the promoter region of the Cmfsg gene of melon, the target point is recognized by a specific sgRNA primer, and is screened by specific primer PCR amplification and sequencing verification. The creation method comprises the following steps: constructing an editing vector of a target gene, introducing the wild-type queen melon by means of an agrobacterium-mediated genetic transformation technology, screening, regeneration and molecular identification to obtain the mutant. The Cmfsg gene has a regulation function on the fruit fur character and the fruit shape index of melon, can realize the direct and rapid creation of the appearance character of melon, overcomes the defects of long breeding period and low efficiency in traditional breeding, and the obtained mutant has stable genetic traits, and can be used as a parent or genetic resource for high-quality breeding of melon.

The invention belongs to the technical field of biology, and discloses a saccharomyces cerevisiae engineering strain for producing naringenin, a construction method and application, the saccharomyces cerevisiae engineering strain contains tyrosine ammonia lyase gene TAL, 4-cumaric acid: coenzyme A ligase gene 4CL, chalcone synthase gene CHS, chalcone isomerase gene CHI, oligopeptide gene RIADn-RGGm and oligopeptide gene RIDD; the ARO10 gene, the FDC1 gene, the PAD1 gene and the PDC5 gene are knocked out. The engineering strain disclosed by the invention can be used for synthesizing naringenin by taking L-tyrosine as a substrate, and the engineering strain is stable in hereditary character and excellent in naringenin yield which can reach 27.45 mg / L; and the fermentation production of the product can be carried out through relatively simple method conditions, so that the method has a very wide application prospect.

The invention relates to the technical field of plant genetic engineering, and discloses a rice brown planthopper resistant gene Bph56, a molecular marker closely linked with the same and application of the rice brown planthopper resistant gene Bph56. The rice Bph56 gene provided by the invention is of great significance for cultivating a new variety of brown planthopper resistant rice. The rice Bph56 gene is successfully cloned through a map-based cloning method and is subjected to function annotation and identification, the gene is completely dominant, the homozygous and heterozygous genotypes endow rice with equal brown planthopper resistance, and the characteristic is beneficial to application of the gene in breeding. On the basis, the Bph56 gene can be introduced into a plant variety susceptible to brown planthopper through traditional breeding technologies such as hybridization, backcross and the like or genetic engineering means, and a novel brown planthopper-resistant plant variety with stable hereditary characters is obtained through cultivation. The invention provides important germplasm resources for effectively reducing insect pests and realizing yield increase and stable yield of crops.

This invention discloses a Fam205a1 Methods for constructing and applying gene knockout mouse models. The construction method utilizes CRISPR / Cas9 gene editing technology, targeting mice. Fam205a1 Gene-designed specific sgRNAs for knockout Fam205a1 The regions of exons 1 through 4 of the gene are obtained. Fam205a1 Gene knockout mice. Male mice in this model exhibit infertility, specifically characterized by impaired spermatogenesis, reduced sperm motility, abnormal sperm morphology (particularly head and acrosome defects), and spermatogenic cell apoptosis. However, the weight and appearance of their testes and epididymis show no significant difference from the wild type. The mouse model constructed in this invention has stable genetic traits and a well-defined phenotype, making it an ideal animal model for studying the mechanisms of idiopathic male infertility, screening drugs for treating male infertility, or developing male contraceptives.

The present application relates to a kind of yunnan ganoderma strain L4741 and its cultivation method, belong to the field of microbial technology.The yunnan ganoderma strain L4741 has been preserved in Guangdong Provincial Microbial Culture Collection Center on December 27, 2021, and the preservation number is GDMCC No:62108.Through collecting and separating wild ganoderma strain L4741 and combining morphological and DNA barcoding technology to determine its taxonomic status, strain culture, biological characteristics and artificial domestication cultivation are carried out, and mature fruiting bodies are obtained after bag material covering soil cultivation.The yunnan ganoderma strain L4741 of the present application has excellent agronomic traits, high and stable yield, bag yield is 61.6g / bag, equivalent to 308kg per mu.Moreover, the content of active substance is higher than that of widely cultivated ganoderma lucidum, the mushroom is neat and has good stress resistance, the genetic traits are stable, and it is easy to popularize and apply.

The application discloses a device for breeding good species of crayfish and application thereof, and the device comprises a population selection unit and a family selection unit; the population selection unit is a cuboid in overall structure, and comprises a population selection water tank, a population selection crayfish tank and a crayfish nest; the population selection crayfish tank is detachably embedded in the population selection water tank, and the crayfish nest is detachably embedded in the sandwiched layer between the population selection crayfish tank and the population selection water tank; the family selection unit is a cylinder in overall structure, and comprises a family selection water tank, a family selection crayfish tank and a crayfish nest; the family selection crayfish tank is detachably embedded in the family selection water tank, and the crayfish nest is detachably embedded in the sandwiched layer between the family selection crayfish tank and the family selection water tank. Advantages of the device are as follows: the device utilizes the self behavior characteristics and external nest position advantages of crayfish to first breed parent individuals with strong bodies and strong resistance, and then remove the nest position advantages to breed individuals with strong reproductive capacity; and the device can obtain crayfish parents with stable genetic traits.

The application discloses a recombinant escherichia coli with high yield of L-tryptophan and application thereof, and belongs to the technical field of bioengineering.The application provides the recombinant escherichia coli with high yield of L-tryptophan to solve the problem that traditional microbial fermentation methods in the prior art depend on known target directional modification, result in a bottleneck in yield improvement of the strain, and cannot meet the industrial high-yield requirement.The preservation number of the recombinant escherichia coli is CGMCC No.38624, glucose is used as a carbon source, the L-tryptophan yield reaches 11.0 g / L after 24 h of fermentation in a 5L bioreactor, the L-tryptophan yield of a starting strain cultured under the same condition is only 3.4 g / L, the strain is stable in genetic traits, high in fermentation efficiency, and can be used for high-efficiency production of L-tryptophan.

By engineering genetic traits associated with photosynthesis in plants, transgenic plants with increased crop yield can be generated, which will ultimately lead to increased food production in the field. To obtain beneficial genetic traits for enhanced photosynthesis, accelerated evolution is applied under salt stress in a cyanobacteria species, Synechococcus elongatus PCC 7942, by conditionally suppressing its mismatch repair system. Through high-throughput screening for biomass accumulation and sucrose production, it is discovered that specific amino acid changes in the pre-Dl protein of Photosystem II (PSII), encoded by the psbA genes, can improve efficiency of photosynthesis under salt stress, as well as pathogen resistance. Next, the same amino acid changes were made in Arabadopsis and proved that photosynthetic efficiency (measured via growth rate) is also increased in this model vascular plant.

The invention is applicable to the technical field of animal model construction, and provides a method for constructing a rabbit growth trait model based on ZDHHC23 gene knockout, and the method comprises the following steps: transcription synthesis of SpG-Cas9 mRNA; the method comprises the following steps: designing and synthesizing a zinc finger structural domain sgRNA of a rabbit ZDHHC23, namely designing an action target sgRNA on a rabbit ZDHHC23 gene aiming at the zinc finger structural domain of the rabbit ZDHHC23; and preparing the ZDHHC23 gene edited rabbit by an embryo microinjection technology. According to the embodiment of the invention, a SpG-Cas9 gene editing technology is utilized, gene knockout is carried out on the zinc finger structural domain of the rabbit ZDHHC23, a new variety of the rabbit with increased weight is obtained, and the method has application value in promoting improvement of genetic characters of the rabbit and improving production performance and economic value of the rabbit.

This invention relates to the field of grape breeding technology, specifically to a rapid breeding method for large-berry, aromatic, and storage-resistant grapes. Through optimization of parent selection, hybridization breeding, and seedling screening and cultivation, the method ensures that the bred grape varieties possess stable genetic traits and high quality. This breeding method can quickly screen grape varieties with excellent traits such as large berries, aroma, and storage resistance, significantly shortening the breeding cycle and improving breeding efficiency. The large-berry, aromatic, and storage-resistant grape varieties bred using this invention have broad application prospects in both fresh consumption and processing, meeting market demands and promoting the development of the grape industry.

The invention discloses a method for quickly transforming spring low-yield tung tree forests, and belongs to the technical field of tung tree grafting. The problems that in aleurites fordii low-yield forest transformation, seedling sowing offspring hereditary characters are unstable, fruiting is late, the economic return period is long, traditional grafted seedling transplanting nursery stock cultivation time is long, improved variety asexual breeding popularization is slow, and production management and protection cost is high are mainly solved. The innovation of the method comprises the overall cooperation of the technical means of treating a seed tree to cultivate robust scions, determining the optimal grafting time, adopting the optimal grafting method, optimizing management after grafting and the like. According to the method, tree vigor recovery, fast stock-scion wound healing and fast growth after low-yield tung tree grafting are achieved for the first time, flowering and fruiting in the current year after grafting can be achieved, the fruiting amount in the next year is remarkably increased, the high-yield period is entered in the third year, the method is particularly suitable for rapid improved variety transformation of tung tree low-yield forest stand, and economic benefits are quite remarkable.

This invention discloses a method for promoting somatic embryogenesis and maturation in Cryptomeria japonica, belonging to the field of somatic embryogenesis technology. The method involves using immature seed embryos as explants to induce embryogenic callus; transferring the proliferated embryogenic callus to a somatic embryogenesis induction medium for further culture to obtain somatic embryos; transferring the somatic embryos to a somatic embryogenesis maturation medium for dark culture to obtain mature cotyledonary embryos; transferring the mature cotyledonary embryos to a somatic embryogenesis basal medium for light culture to obtain somatic embryonic seedlings; and hardening off the seedlings before transplanting to obtain regenerated plants. The results of this application show that embryogenic callus can be stably obtained from different families, with high somatic embryogenesis maturation capacity and rate, enabling the cultivation of a large number of high-quality seedlings with stable genetic traits in a short time; avoiding the phenotypic segregation problem caused by gene recombination during seed propagation, and ensuring that offspring plants inherit the superior characteristics of the parents.

This invention relates to the field of plant molecular breeding technology, and discloses a tomato NOR‑like1 The application of genes in breeding tomato varieties that are easy to deseed and process, the aforementioned NOR‑like1 The gene is an encoding a NAC transcription factor, the coding sequence of which is shown in SEQ ID NO: 1, or a nucleic acid sequence that has at least 90% sequence identity with the sequence shown in SEQ ID NO: 1 and has the function of regulating the development of the trichomes of tomato seed coat; by reducing or eliminating the above... NOR‑like1 Breeding goals are achieved by adjusting gene expression levels or protein function in tomato plants. The resulting tomato plants exhibit the absence of seed coat hairs on the seed surface, preventing adhesion between adjacent seeds, and producing large fruits. This invention fundamentally eliminates seed coat hairs on tomato seeds at the genetic level, and has broad application potential in breeding tomato varieties with easily separable seeds from the pulp and easily separable seeds.

The application belongs to the technical field of PCR detection, and particularly relates to a method and system for individual genetic trait analysis by integrated multiplex PCR. The method comprises starting a multiplex PCR reaction for a target genetic trait in an integrated multiplex PCR system, and simultaneously starting a real-time fluorescence signal monitoring process for each fluorescence detection channel in the multiplex PCR reaction process; calculating a set of key characteristic parameters of each detection channel based on early cycle fluorescence data obtained by the real-time fluorescence signal monitoring process; matching each set of key characteristic parameters with a preset adaptive rule set, and generating an adjustment instruction for a subsequent process of the multiplex PCR reaction based on a matching result; and compiling and sending the adjustment instruction to a control center of the integrated multiplex PCR system in real time. The application solves the problem of amplification failure or low efficiency caused by the fact that a fixed process cannot adapt to sample differences.

The invention discloses a method for optimizing tissue culture, rapid propagation and seedling culture of valerian neolastoma. The method comprises the steps of explant selection, explant disinfection, bud induction, bud proliferation, subculture, rooting culture, test-tube plantlet transplanting and the like, in the process, by optimizing a tissue culture scheme, a culture medium of the same formula is used during bud induction, bud proliferation and cluster bud subculture, the convention of'replacement of the culture medium in stages' in traditional tissue culture is broken, and the tissue culture efficiency is improved. The defects of high cost, high error and high variation of traditional tissue culture are overcome, the proliferation multiple can reach 4.0-5.0, and compared with the prior art, the efficiency is higher; the valerian anemone seedlings cultivated by the method are stable in hereditary character, maintain the characteristics of parents, have the advantages of invariance, low investment, high yield, short period and the like, and can realize large-scale seedling production.

The invention relates to the technical field of intelligent animal husbandry, and discloses a hereditary character monitoring and breeding device for early breeding of calves, the hereditary character monitoring and breeding device comprises an electric guide rail and a food bowl, the food bowl is mounted on a moving seat of the electric guide rail, the two sides of the interior of the food bowl are fixedly connected with inclined plane stop blocks, and a waste discharge port is formed below the inclined plane stop blocks in a penetrating manner; the inner side of the food bowl is slidably connected with a scraping plate, the top of the scraping plate is attached to the bottom of the slope stop block, the left side and the right side of the scraping plate are each fixedly connected with two pull rings, pull ropes are arranged outside the pull rings, and the left side and the right side of the food bowl are each provided with two guide wheels. The motor, the winding rod and the pull rope are arranged to drive the scraping plate, the scraping plate can automatically clear residual feed or waste in the food bowl through the waste discharge port under the guidance of the inclined plane stop block, automatic cleaning of the food bowl is achieved, the intensity and frequency of manual intervention are reduced, sanitation of the feeding environment is ensured, and the feeding efficiency is improved. The calves are prevented from eating the polluted feed.

This application provides a method and system for predicting aquatic genetic traits based on multi-semantic feature fusion and knowledge distillation, relating to the field of biogenetics. The method includes: acquiring SNP data and phenotypic labels; constructing six-dimensional semantic features and using CLIP cross-modal contrastive learning to obtain enhanced feature embeddings; constructing a GIA-Net teacher network, whose graph branch captures local linkage features and sequence branch captures global long-range dependency features based on Mamba, dynamically fusing these features to generate prediction results and site importance scores; constructing a lightweight BioMamba student network, trained through multi-level knowledge distillation; and using the trained student network to predict traits. This application achieves a systematic integration of computational efficiency and genetic analysis capabilities, providing an efficient and secure digital decision-making tool for disease-resistant breeding of plants and animals and for mining key variation sites.

The application belongs to the technical field of biotechnology, and particularly relates to an engineered bacterium for high-yield L-tryptophan and a construction method and application thereof. The engineered bacterium provided by the application is constructed by gene modification based on an Escherichia coli as a starting strain, can produce L-tryptophan at a high yield, has a high conversion rate, a short fermentation cycle, and stable genetic traits, and does not need to add an inducer and an antibiotic in a fermentation production process, so that the fermentation process is simplified, and the engineered bacterium is more suitable for industrialized production of L-tryptophan.

The present invention relates to plants exhibiting one or more useful traits and methods of producing such plants, such as increased yield, increased biomass, drought tolerance, salt tolerance, increased lignin yield, biological stress tolerance, abiotic stress tolerance, increased growth rate, or beneficial root structures. In particular, the present invention relates to plants, plant cells, leaves, stems, flowers, and seeds that are not genetically modified and exhibit useful traits, which are capable of conferring one or more useful traits to offspring including cells, leaves, stems, flowers, and seeds; methods of producing and using the plants and plant parts; and products of these plants and plant parts, including processed products, such as food, feed or diet.

The application discloses a melon mutant and variety breeding technology with a leaf stalk and a main stem included at an angle less than 45 degrees, relates to the technical field of melon variety breeding, and comprises the following steps: S1, screening excellent melon germplasm resources with inherent genetic traits of small leaf blades and upright leaf stalks as basic breeding parents, and simultaneously considering comprehensive agronomic traits, disease resistance traits and fruit quality traits of the parents; S2, separating offspring populations by means of artificial hybridization or continuous selfing of the screened breeding parents, and constructing a genetic variation basic population; and S3, under the condition of high-density standardized planting of facilities. The application breeds a new melon variety suitable for high-density cultivation, improves land utilization efficiency, group photosynthetic efficiency and unit area yield, simultaneously improves field ventilation and light penetration conditions, and reduces the risk of disease occurrence. Moreover, due to the fact that the outward expansion degree of the whole plant is obviously reduced, the row width is increased, which is beneficial to mechanical spraying, fertilization and weeding operations, and a large number of labor is reduced.

The present application belongs to the technical field of genetics and image intelligent processing, and particularly relates to a Mendelian fruit fly genetic trait batch identification method based on a convolutional neural network. The method comprises the following steps: using a mobile terminal device to collect fruit fly images, manually labeling fruit fly positioning frames, fruit fly multi-traits, and fruit fly structure key point data sets; using a random enhancement and progressive enhancement method to train a model, using neural network quantification technology to make the model more suitable for mobile terminal rapid operation; using a key point auxiliary classification method to improve the model interpretability of the long-small wing task; positioning the position of each fruit fly in the image through a target detection model on the mobile terminal, and then distinguishing the long-small wing, red-white eye and female-male traits of each fruit fly through a multi-classification model. The present application can greatly facilitate the work of experimenters, reduce manual repetitive labor, save a large amount of time, and exceed the accuracy of manual classification in various trait classification tasks, thereby playing a standardization role.

The application discloses an engineered Trichoderma reesei strain with high yield of beta-glucosidase, and a construction method and application thereof. In view of the problems of low beta-glucosidase activity of a natural cellulase system of Trichoderma reesei in the prior art and easy accumulation of cellobiose to cause feedback inhibition, the application takes Trichoderma reesei RUT-C30 as a starting strain, and through three-step progressive operation, firstly, a beta-glucosidase gene expression cassette derived from Aspergillus niger is integrated into a URA5 gene locus; then, an expression cassette containing a URA5 back complement unit and a BGL gene is constructed and integrated into an ACE1 gene site; finally, a transcription activator XYR1 expression cassette is integrated into a PEP1 site. Through the above iterative modification, an engineered strain with stable genetic traits, capable of simultaneously high-level secretion of beta-glucosidase and filter paper enzyme is obtained. It is verified through experiments that the beta-glucosidase activity of the engineered strain can be up to 80.6 IU / mL, which is 268.3 times that of the starting strain.

The application provides a method for cultivating Zanthoxylum ailanthoides grafted seedlings by using Asparagus cochinchinensis cutting stock, and relates to the technical field of Zanthoxylum ailanthoides grafting. The Zanthoxylum ailanthoides is grafted by using Asparagus cochinchinensis cutting seedlings as stock, the stock is wrapped with the cutting end of the stock after being soaked with absorbent cotton to absorb nutrient solution containing glutamic acid sodium and sodium tripolyphosphate, and the upper end of the Zanthoxylum ailanthoides scion is treated by punching, and then the routine management of cutting is carried out. The application makes up for the shortcomings of the existing grafting seedling cultivation technology, adopts the Asparagus cochinchinensis cutting seedling which is easy to obtain and stable in genetic traits to carry out Zanthoxylum ailanthoides grafting breeding, can stably improve the grafting survival rate of the Zanthoxylum ailanthoides scion and the Asparagus cochinchinensis stock, accelerate the growth rate of the grafted young tree seedling stage, improve the growth index and stress resistance of the seedling, and overall improve the cultivation quality and utilization value of the grafted seedling.

This invention relates to a method based on argB A method for constructing a high-arginine-producing genetically engineered strain using mutants, and the application of this genetically engineered strain in the microbial fermentation production of arginine. The genetically engineered strain was obtained by performing the following gene editing on its genome, starting with Corynebacterium glutamicum ATCC 13032; this invention first involves knocking out the gene encoding the arginine biosynthesis repressor protein. argR and last Relieve transcriptional repression; knock out the gene encoding glutamate kinase. proB Blocking the competitive pathway for the synthesis of the byproduct L-proline; knocking out global nitrogen metabolism regulators. amtR To relieve nitrogen metabolism restriction and enhance nitrogen source supply; to knock out the gene encoding the mechanosensitive channel protein. yggB By altering cell membrane permeability and reducing the leakage of byproducts (such as glutamate), it indirectly promotes arginine accumulation; based on the knockout of the original proB, ldh Replace with Ptac- argB *(T94S, I158V, R273K) strains were constructed to reduce lactic acid byproducts while resisting feedback inhibition; finally, the L-arginine exporter gene was overexpressed via plasmid. lysE By combining the above modification strategies, a genetically engineered strain ARG6-Ptac-, which produces high levels of arginine, was constructed. argB * / pXMJ19- lysE This engineered strain exhibits excellent L-arginine production capacity in both shake flask and fermenter scales (10.3 g / L in shake flask and 105.2 g / L in fed-batch fermentation), and its genetic traits are stable, making it suitable for industrial production.

The application discloses a silkworm variety molecular breeding method against pyrethroid pesticides, and adopts a strategy of combining genetic breeding with molecular marker assisted breeding, and has the following advantages: (1) the molecular marker breeding method has the advantages that genetic traits of the silkworm variety of Su Hao and Zhong Ye against pyrethroid pesticides are stable, and the silkworm variety is obtained by combining molecular markers, hybridization, backcrossing, single moth directional breeding and other methods and production practice; (2) the silkworm variety of Su Hao and Zhong Ye against pyrethroid pesticides obtained by the method has obvious economic benefits in subsequent production practice, and the genetic traits are stable; (3) the method can be popularized to the introduction of pyrethroid pesticide resistance traits of other practical silkworm varieties.

The invention discloses a culture medium for tissue culture and rapid propagation of Bo-chrysanthemum and a tissue culture and rapid propagation method, and aims to solve the problems of low efficiency, easiness in carrying viruses and germplasm degeneration of a traditional propagation mode. The method comprises the following steps: taking stem tips of underground foot buds of Bo-chrysanthemum as explants, sterilizing, inoculating into an MS culture medium containing specific hormones, and sequentially performing primary induction, subculture multiplication and rooting culture to obtain complete test-tube plantlets. And finally transplanting the seedlings to a field through gradual seedling hardening and special matrix transition. According to the method, standardization and high efficiency of the breeding process are achieved, the breeding coefficient is high, the production period is short, seedling detoxification is thorough, genetic characters are consistent, the transplanting survival rate is high, transplanting and harvesting can be conducted in the current year, the quality, yield and production benefits of bo-chrysanthemum seedlings are remarkably improved, and the method is suitable for large-scale application and popularization.

The present application relates to the technical field of plant genetic engineering, and discloses a rice brown planthopper resistance gene Bph56 and a closely linked molecular marker and application thereof. The rice gene provided by the present application has important significance for cultivating new rice varieties resistant to brown planthopper. The present application successfully clones the rice gene by a map-based cloning method, and performs functional annotation and identification on the gene. The gene exhibits complete dominance, and both homozygous and heterozygous genotypes of the gene confer the rice with equal resistance to brown planthopper, which is beneficial to the application of the gene in breeding. Based on this, the gene can be introduced into plant varieties susceptible to brown planthopper by traditional breeding techniques such as hybridization and backcrossing or genetic engineering means, and a new variety of plants resistant to brown planthopper with stable genetic traits can be cultivated. The present application provides important germplasm resources for effectively reducing insect pests and realizing crop yield increase and yield stability. Bph56 Bph56 Bph56 ​​​

The invention discloses a character-stable Chinese holly propagation method based on an apomixis phenomenon, and relates to the technical field of Chinese holly breeding and propagation. According to the method, fine flower thinning is carried out on screened female ilex cornuta plants with excellent characters before flowering of the female ilex cornuta plants; bagging and isolating immediately after flower thinning so as to completely eradicate external pollen pollution; after fruit setting, the bagging type is changed, and a protective agent is sprayed, so that physiological fruit falling is prevented; and finally, harvesting seeds and sowing to obtain a clonal offspring with the genetic character highly consistent with that of the female parent. According to the method, bagging isolation and physiological regulation are combined, the apomixis characteristic of the ilex cornuta is effectively utilized, and breeding with stable characters of excellent single plants is achieved. The method has the remarkable advantages of simplicity and convenience in operation, low cost, high propagation efficiency and stable progeny characters, and is suitable for new Chinese holly variety breeding, rapid propagation and new plant variety protection.