79 results about "Genetic traits" patented technology

The invention is applicable to the technical field of rabbit breeding, and provides a method for increasing the body weight of a rabbit based on a gene editing technology, which comprises the following steps: designing an action target sgRNA on a rabbit NR6A1 gene aiming at an NR6A1 p.P192S site; uniformly mixing the synthesized sgRNA and SpRY-CBE, injecting fertilized eggs, transplanting the fertilized eggs into fallopian tubes of receptor female rabbits, and feeding the receptor female rabbits until production; dNA is extracted from produced young rabbit ear tissues, PCR and sequencing are carried out, and if C at the fifth site in NR6A1 gene target sites is mutated into T, it is proved that single base mutation is obtained, and the NR6A1 gene edited rabbit is obtained. According to the invention, the rabbit variety with increased weight is successfully cultivated; a new thought of animal genetic breeding is developed by utilizing a gene editing technology, the method has application values in promoting improvement of genetic characters of rabbits and improving production performance and economic value of the rabbits, and powerful support is provided for high-quality development of the animal breeding industry in China.

By engineering genetic traits associated with photosynthesis in plants, transgenic plants with increased crop yield can be generated, which will ultimately lead to increased food production in the field. To obtain beneficial genetic traits for enhanced photosynthesis, accelerated evolution is applied under salt stress in a cyanobacteria species, Synechococcus elongatus PCC 7942, by conditionally suppressing its mismatch repair system. Through high-throughput screening for biomass accumulation and sucrose production, it is discovered that specific amino acid changes in the pre-Dl protein of Photosystem II (PSII), encoded by the psbA genes, can improve efficiency of photosynthesis under salt stress, as well as pathogen resistance. Next, the same amino acid changes were made in Arabadopsis and proved that photosynthetic efficiency (measured via growth rate) is also increased in this model vascular plant.

A system and method for developing a personalized program for the identification and breeding of animals of superior genetic merit for a variety of traits.

The invention discloses a gene detection system which comprises a sample collection module, a gene extraction module, a sequencing module, a data analysis module and a report generation module. The gene extraction module is used for extracting high-quality DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) from a sample, and the data analysis module is used for processing, analyzing and explaining original data obtained by sequencing by integrating various bioinformatics algorithms and tools. The game theory is integrated into the whole genome association analysis GWAS model to improve the whole genome association analysis GWAS model, the game theory is combined to enable the SNP sites to be more and more detailed, the relevance between deeper hereditary characters and defective genes can be mined, the false positive probability of the result obtained by the association model is smaller, and the accuracy of the result obtained by the association model is improved. A genetic-defect gene association map is constructed according to a whole genome association analysis GWAS model fused with the game theory thought, a defect gene risk prediction model is trained, and the most possible defect gene type is predicted according to genetic characteristics, so that the accuracy is greatly improved.

The present invention discloses an SNP molecular marker affecting the litter interval trait. This SNP molecular marker is a C>A base mutation at the position of 29,375,835 bp on chromosome 7 of the international pig genome Ensemble Sscrofa11.1 version. The gene fragment of this SNP molecular marker locus is as shown in SEQ ID NO:1. The mutation site in the nucleotide sequence of SEQ ID NO:1 is the 161st base M, and M is selected from base C or A. This molecular marker is applied to the breeding of sow litter interval traits, the cultivation of high-fertility sow lines, and the improvement of the genetic traits of sow population fecundity. It can greatly shorten the litter interval, thereby significantly shortening the production process, reducing the risk of sow dystocia, reducing the harm to sows and improving the survival rate of piglets, improving the reproductive performance of sows / populations, and improving production efficiency.

This invention discloses a two-step method for rapid propagation of cinnamon buds, comprising the following steps: Step 1: Collecting cinnamon buds as explants, sterilizing and preventing browning of the explants, and then inoculating them into an induction medium for induction culture to obtain clustered buds. Culture is continued until the proliferation coefficient of the clustered buds reaches 5 or higher. Step 2: Cutting off healthy single buds with a height >2cm, transferring them to a rooting induction medium, and continuing culture to obtain rooted tissue culture seedlings with a bud height of 3-6cm and a root length of 1-2cm. These seedlings are then hardened off and transplanted. The clustered buds produced by this invention are of high quality and abundant quantity, with stable genetic traits and a proliferation coefficient of 5 or higher. This invention is simple to operate, has a short culture cycle, and low production costs, resulting in significant economic, ecological, and social benefits.

The invention belongs to the technical field of idesia polycarpa planting, and particularly relates to a layering seedling forming method for idesia polycarpa. The moss is treated by using the aqueous solution of naphthylacetic acid and indolebutyric acid to form a rooting medium for layering treatment of branches, so that the rooting rate of current-year branches is 100%, the number of adventitious roots of grown seedlings is large (more than 5), the quality is good (white and 1-2mm), and the transplanting survival rate is more than 90%. And the prepared seedlings grow fast, bear fruits early and have stable hereditary characters. Besides, the method does not need to spend a large amount of time for training professional technicians, common labor service temporary teaching can be completed, labor cost is reduced, the used layering substrate is available, purchasing, allocation and transportation troubles are avoided, layering boxes and coiled aluminum wires are easy to purchase and easy to obtain, cost is low, and seedling raising economic cost is integrally reduced.

This invention provides a rice gene Bph54 for resistance to brown planthopper and its application. The rice provided by this invention... Bph54 Genes are of great significance for breeding new rice varieties resistant to brown planthoppers. This invention successfully cloned the rice Bph54 gene using map-based cloning, and performed functional annotation and identification. This gene exhibits complete dominance, and both homozygous and heterozygous genotypes confer equal resistance to brown planthoppers in rice. This characteristic is beneficial for the application of this gene in breeding. Based on this, the Bph54 gene can be introduced into brown planthopper-susceptible plant varieties through traditional breeding techniques such as hybridization and backcrossing, or through genetic engineering, to cultivate new varieties of plants resistant to brown planthoppers with stable genetic traits. This invention provides important germplasm resources for effectively reducing insect damage and achieving increased and stable crop yields.

The application belongs to the technical field of genetic engineering, and particularly relates to a gRNA targeting Bombyx mori sericin 1 gene, a method for preparing coarse silk and application. The target sequence of the gRNA is shown as SEQ ID NO:1. The method for preparing coarse silk comprises the following steps: 1) constructing a gRNA expression vector targeting the Bombyx mori BmSer1 gene, and preparing a gRNA transgenic Bombyx mori; 2) crossing the gRNA transgenic Bombyx mori and Cas9 transgenic Bombyx mori, and screening to obtain double transgenic individuals with green fluorescence in eyes and bodies; 3) screening to obtain a heterozygous mutant Bombyx mori strain with the BmSer1 gene knocked out through typing detection; and 4) obtaining coarse silk by feeding the transgenic Bombyx mori. The genetic editing technology is used to change the genetic traits of Bombyx mori, and the obtained silk has a larger diameter, a lower sericin rate, better water resistance and durability.

The application discloses a Cmfsg gene mutant of melon and a creation method and application thereof, and belongs to the technical field of plant genetic engineering. The mutant is obtained by CRISPR / Cas9 gene editing technology to direct mutation of the coding region or the promoter region of the Cmfsg gene of melon, the target point is recognized by a specific sgRNA primer, and is screened by specific primer PCR amplification and sequencing verification. The creation method comprises the following steps: constructing an editing vector of a target gene, introducing the wild-type queen melon by means of an agrobacterium-mediated genetic transformation technology, screening, regeneration and molecular identification to obtain the mutant. The Cmfsg gene has a regulation function on the fruit fur character and the fruit shape index of melon, can realize the direct and rapid creation of the appearance character of melon, overcomes the defects of long breeding period and low efficiency in traditional breeding, and the obtained mutant has stable genetic traits, and can be used as a parent or genetic resource for high-quality breeding of melon.

The invention belongs to the technical field of biology, and discloses a saccharomyces cerevisiae engineering strain for producing naringenin, a construction method and application, the saccharomyces cerevisiae engineering strain contains tyrosine ammonia lyase gene TAL, 4-cumaric acid: coenzyme A ligase gene 4CL, chalcone synthase gene CHS, chalcone isomerase gene CHI, oligopeptide gene RIADn-RGGm and oligopeptide gene RIDD; the ARO10 gene, the FDC1 gene, the PAD1 gene and the PDC5 gene are knocked out. The engineering strain disclosed by the invention can be used for synthesizing naringenin by taking L-tyrosine as a substrate, and the engineering strain is stable in hereditary character and excellent in naringenin yield which can reach 27.45 mg / L; and the fermentation production of the product can be carried out through relatively simple method conditions, so that the method has a very wide application prospect.

The invention relates to the technical field of plant genetic engineering, and discloses a rice brown planthopper resistant gene Bph56, a molecular marker closely linked with the same and application of the rice brown planthopper resistant gene Bph56. The rice Bph56 gene provided by the invention is of great significance for cultivating a new variety of brown planthopper resistant rice. The rice Bph56 gene is successfully cloned through a map-based cloning method and is subjected to function annotation and identification, the gene is completely dominant, the homozygous and heterozygous genotypes endow rice with equal brown planthopper resistance, and the characteristic is beneficial to application of the gene in breeding. On the basis, the Bph56 gene can be introduced into a plant variety susceptible to brown planthopper through traditional breeding technologies such as hybridization, backcross and the like or genetic engineering means, and a novel brown planthopper-resistant plant variety with stable hereditary characters is obtained through cultivation. The invention provides important germplasm resources for effectively reducing insect pests and realizing yield increase and stable yield of crops.

This invention discloses a Fam205a1 Methods for constructing and applying gene knockout mouse models. The construction method utilizes CRISPR / Cas9 gene editing technology, targeting mice. Fam205a1 Gene-designed specific sgRNAs for knockout Fam205a1 The regions of exons 1 through 4 of the gene are obtained. Fam205a1 Gene knockout mice. Male mice in this model exhibit infertility, specifically characterized by impaired spermatogenesis, reduced sperm motility, abnormal sperm morphology (particularly head and acrosome defects), and spermatogenic cell apoptosis. However, the weight and appearance of their testes and epididymis show no significant difference from the wild type. The mouse model constructed in this invention has stable genetic traits and a well-defined phenotype, making it an ideal animal model for studying the mechanisms of idiopathic male infertility, screening drugs for treating male infertility, or developing male contraceptives.

This invention discloses an epigenetic marker related to the variation of body color black spots in red tilapia during overwintering, and further utilizes this epigenetic marker to develop detection primers and a method for determining the variation of body color black spots in red tilapia during overwintering. This method can be applied to the screening of red tilapia exhibiting body color black spot variation during overwintering, selecting genetically stable red tilapia as broodstock for epigenetic breeding. Therefore, by utilizing an epigenetic marker related to the variation of body color black spots in red tilapia during overwintering, a new approach and method are provided for its epigenetic breeding, offering a new scheme for improving the variation of body color black spots in red tilapia.

The present invention relates to the technical field of pig molecular marker screening, and more particularly to a single-nucleotide polymorphism (SNP) molecular marker located on chromosome 5 and associated with pig lean meat percentage, and its application. This SNP molecular marker corresponds to a T>C mutation at bp 64,865,623 and an A>G mutation at bp 64,865,624 on chromosome 5 of the International Porcine Genome Version 11.1 reference sequence. These two adjacent sites are the highest points in the GWAS and are highly linked to surrounding SNP sites. Application of this marker in genotyping, genetic breeding, and other areas can address current technical issues, such as the lack of genetic traits associated with pig lean meat percentage.

The invention relates to the technical field of rapid propagation of Chinese herbal medicines, in particular to a tissue culture and rapid propagation method for Pangolin by utilizing stem segments to induce cluster buds and application of the tissue culture and rapid propagation method. The stem segments with buds at the top ends of branches of Pangolin and terminal buds are selected as explants, and the explants are inoculated to a cluster bud induction culture medium to be cultured after being cleaned and disinfected; and inoculating the induced cluster buds on a cluster bud proliferation culture medium for culturing, inoculating the proliferated cluster buds on a rooting culture medium for culturing, and directly transplanting and domesticating. According to the method disclosed by the invention, a proper concentration combination of 6-BA, NAA, IAA and silver nitrate is adopted during primary induction, cluster buds can be directly induced, the production period is shortened, the production cost is reduced, the plant inheritable character is stable, the excellent quality and characteristics of stock plants are reserved, GA3 and TIBA are combined in the proliferation process, seedlings are more robust, and the survival rate of the seedlings is increased by combining with a proper concentration combination of 1 / 2 MS, IBA and SA. The method can promote the formation of base root primordia and the growth of root systems, increase the stress resistance of plants, and can form qualified rooting seedlings within 15-20 days.

The present application relates to a kind of yunnan ganoderma strain L4741 and its cultivation method, belong to the field of microbial technology.The yunnan ganoderma strain L4741 has been preserved in Guangdong Provincial Microbial Culture Collection Center on December 27, 2021, and the preservation number is GDMCC No:62108.Through collecting and separating wild ganoderma strain L4741 and combining morphological and DNA barcoding technology to determine its taxonomic status, strain culture, biological characteristics and artificial domestication cultivation are carried out, and mature fruiting bodies are obtained after bag material covering soil cultivation.The yunnan ganoderma strain L4741 of the present application has excellent agronomic traits, high and stable yield, bag yield is 61.6g / bag, equivalent to 308kg per mu.Moreover, the content of active substance is higher than that of widely cultivated ganoderma lucidum, the mushroom is neat and has good stress resistance, the genetic traits are stable, and it is easy to popularize and apply.

A system and method for developing a personalized program for the identification and breeding of animals of superior genetic merit for a variety of traits.

The application discloses a device for breeding good species of crayfish and application thereof, and the device comprises a population selection unit and a family selection unit; the population selection unit is a cuboid in overall structure, and comprises a population selection water tank, a population selection crayfish tank and a crayfish nest; the population selection crayfish tank is detachably embedded in the population selection water tank, and the crayfish nest is detachably embedded in the sandwiched layer between the population selection crayfish tank and the population selection water tank; the family selection unit is a cylinder in overall structure, and comprises a family selection water tank, a family selection crayfish tank and a crayfish nest; the family selection crayfish tank is detachably embedded in the family selection water tank, and the crayfish nest is detachably embedded in the sandwiched layer between the family selection crayfish tank and the family selection water tank. Advantages of the device are as follows: the device utilizes the self behavior characteristics and external nest position advantages of crayfish to first breed parent individuals with strong bodies and strong resistance, and then remove the nest position advantages to breed individuals with strong reproductive capacity; and the device can obtain crayfish parents with stable genetic traits.

The application discloses a recombinant escherichia coli with high yield of L-tryptophan and application thereof, and belongs to the technical field of bioengineering.The application provides the recombinant escherichia coli with high yield of L-tryptophan to solve the problem that traditional microbial fermentation methods in the prior art depend on known target directional modification, result in a bottleneck in yield improvement of the strain, and cannot meet the industrial high-yield requirement.The preservation number of the recombinant escherichia coli is CGMCC No.38624, glucose is used as a carbon source, the L-tryptophan yield reaches 11.0 g / L after 24 h of fermentation in a 5L bioreactor, the L-tryptophan yield of a starting strain cultured under the same condition is only 3.4 g / L, the strain is stable in genetic traits, high in fermentation efficiency, and can be used for high-efficiency production of L-tryptophan.

By engineering genetic traits associated with photosynthesis in plants, transgenic plants with increased crop yield can be generated, which will ultimately lead to increased food production in the field. To obtain beneficial genetic traits for enhanced photosynthesis, accelerated evolution is applied under salt stress in a cyanobacteria species, Synechococcus elongatus PCC 7942, by conditionally suppressing its mismatch repair system. Through high-throughput screening for biomass accumulation and sucrose production, it is discovered that specific amino acid changes in the pre-Dl protein of Photosystem II (PSII), encoded by the psbA genes, can improve efficiency of photosynthesis under salt stress, as well as pathogen resistance. Next, the same amino acid changes were made in Arabadopsis and proved that photosynthetic efficiency (measured via growth rate) is also increased in this model vascular plant.

The present invention belongs to the technical field of plant cultivation, and particularly relates to a method for rapid tissue culture propagation of alpine wild Kentucky bluegrass. The tissue culture medium for alpine wild Kentucky bluegrass of the present invention comprises a primary medium, a multiple shoot induction medium and a rooting medium; 6-BA and 2,4-D are added to the primary medium and the multiple shoot induction medium; NAA is added to the rooting medium. Under the combined action of 6-BA, 2,4-D and NAA in each medium, the seeds of wild Kentucky bluegrass are induced to germinate and tissue differentiation occurs, obtaining a large number of multiple shoots and tissue culture seedlings, which can not only maintain the original genetic traits and bio-ecological characteristics, but also rapidly propagate to obtain seedlings, realizing the rapid domestication and breeding of native grass seeds, and providing effective technical support for the preservation of excellent traits of wild grass seeds and the breeding of new varieties.

The invention is applicable to the technical field of animal model construction, and provides a method for constructing a rabbit growth trait model based on ZDHHC23 gene knockout, and the method comprises the following steps: transcription synthesis of SpG-Cas9 mRNA; the method comprises the following steps: designing and synthesizing a zinc finger structural domain sgRNA of a rabbit ZDHHC23, namely designing an action target sgRNA on a rabbit ZDHHC23 gene aiming at the zinc finger structural domain of the rabbit ZDHHC23; and preparing the ZDHHC23 gene edited rabbit by an embryo microinjection technology. According to the embodiment of the invention, a SpG-Cas9 gene editing technology is utilized, gene knockout is carried out on the zinc finger structural domain of the rabbit ZDHHC23, a new variety of the rabbit with increased weight is obtained, and the method has application value in promoting improvement of genetic characters of the rabbit and improving production performance and economic value of the rabbit.

The invention provides a breeding method for brewing special type husked glutinous barley, and belongs to the technical field of plant breeding. In order to combine the advantages of the waxy barley and the hulled barley, enable the waxy barley to have hulls and accelerate the breeding process, a main spike mixed selection method is adopted, naked-grain single plants and plant rows with naked grains are eliminated at a time from character separation of the F2 generation, the field selection target is clear, the workload is greatly reduced, the breeding work efficiency is improved, and the breeding cost is reduced. And meanwhile, main ears are subjected to consecutive mixed planting for several years, heterozygotes are fully separated and purified, the probability of selecting and remaining single plants with skins is higher, and invalid selections are reduced to a certain extent. In addition, the breeding method has the advantages that the field population in the early stage of growth is small, water and fertilizer supply of the main spikes is sufficient, genetic characters can be well expressed, and misjudgment caused by uneven tillering growth in the middle and later periods due to the overlarge population is avoided.

This invention relates to the field of grape breeding technology, specifically to a rapid breeding method for large-berry, aromatic, and storage-resistant grapes. Through optimization of parent selection, hybridization breeding, and seedling screening and cultivation, the method ensures that the bred grape varieties possess stable genetic traits and high quality. This breeding method can quickly screen grape varieties with excellent traits such as large berries, aroma, and storage resistance, significantly shortening the breeding cycle and improving breeding efficiency. The large-berry, aromatic, and storage-resistant grape varieties bred using this invention have broad application prospects in both fresh consumption and processing, meeting market demands and promoting the development of the grape industry.

The invention discloses a method for increasing the yield of astaxanthin in phaffia rhodozyma by using a synergistic gene expression strategy. Firstly, the invention relates to application of a non-homologous terminal connection protein gene and an iron reductase gene in improving the yield of astaxanthin in phaffia rhodozyma; the method comprises the following steps: introducing a non-homologous terminal junction protein gene and an iron reductase gene into phaffia rhodozyma cells, cooperatively expressing the non-homologous terminal junction protein gene and the iron reductase gene, and fermenting by using the phaffia rhodozyma to produce the astaxanthin. According to the method disclosed by the invention, the biomass and the astaxanthin yield of the phaffia rhodozyma are improved by synergistically overexpressing the non-homologous terminal connecting protein and the iron reductase gene in phaffia rhodozyma cells; the phaffia rhodozyma high-yield astaxanthin strain is constructed through a gene overexpression technology, has the characteristics of genetic characters and stable astaxanthin fermentation yield, and is relatively high in feasibility.

The invention discloses a method for quickly transforming spring low-yield tung tree forests, and belongs to the technical field of tung tree grafting. The problems that in aleurites fordii low-yield forest transformation, seedling sowing offspring hereditary characters are unstable, fruiting is late, the economic return period is long, traditional grafted seedling transplanting nursery stock cultivation time is long, improved variety asexual breeding popularization is slow, and production management and protection cost is high are mainly solved. The innovation of the method comprises the overall cooperation of the technical means of treating a seed tree to cultivate robust scions, determining the optimal grafting time, adopting the optimal grafting method, optimizing management after grafting and the like. According to the method, tree vigor recovery, fast stock-scion wound healing and fast growth after low-yield tung tree grafting are achieved for the first time, flowering and fruiting in the current year after grafting can be achieved, the fruiting amount in the next year is remarkably increased, the high-yield period is entered in the third year, the method is particularly suitable for rapid improved variety transformation of tung tree low-yield forest stand, and economic benefits are quite remarkable.

The present invention provides a method for breeding broodstock of whiteleg shrimp, which comprises screening healthy broodstock free of specific pathogens, grading them according to growth rate, body shape and reproductive capacity, and breeding them in an optimized breeding environment, including precise control of water temperature, salinity and feed ratio, obtaining shrimp seedlings through regular health monitoring and genetic trait recording, combining artificial insemination or natural breeding, and screening for genetic traits. After the shrimp seedlings are cultivated to the P10 stage, they are screened again through cage grading to ensure that the shrimp seedlings are of consistent specifications. High-quality broodstock are used for the next generation of breeding, forming a closed-loop breeding system. In addition, an animal model is established through computer software, the growth traits of the family are evaluated, and the breeding plan is continuously optimized. This method ensures the genetic advantage and health status of the broodstock by comprehensively using biostatistics, molecular marker technology and morphological and physiological evaluation, providing an efficient and sustainable broodstock breeding strategy for the whiteleg shrimp farming industry.

This invention discloses a method for promoting somatic embryogenesis and maturation in Cryptomeria japonica, belonging to the field of somatic embryogenesis technology. The method involves using immature seed embryos as explants to induce embryogenic callus; transferring the proliferated embryogenic callus to a somatic embryogenesis induction medium for further culture to obtain somatic embryos; transferring the somatic embryos to a somatic embryogenesis maturation medium for dark culture to obtain mature cotyledonary embryos; transferring the mature cotyledonary embryos to a somatic embryogenesis basal medium for light culture to obtain somatic embryonic seedlings; and hardening off the seedlings before transplanting to obtain regenerated plants. The results of this application show that embryogenic callus can be stably obtained from different families, with high somatic embryogenesis maturation capacity and rate, enabling the cultivation of a large number of high-quality seedlings with stable genetic traits in a short time; avoiding the phenotypic segregation problem caused by gene recombination during seed propagation, and ensuring that offspring plants inherit the superior characteristics of the parents.

The present invention is applicable to the field of rabbit breeding technology and provides a method for increasing rabbit weight based on gene editing technology, comprising: designing a target sgRNA for the NR6A1 p.P192S site on the rabbit NR6A1 gene; mixing the synthesized sgRNA and SpRY‑CBE, injecting the resultant sgRNA into a fertilized egg, transplanting the mixture into the oviduct of a recipient female rabbit, and raising the recipient female rabbit until birth; extracting DNA from the ear tissue of the resulting young rabbit, performing PCR and sequencing, and if the C at position 5 in the NR6A1 gene target site mutates to T, a single base mutation is obtained, i.e., an NR6A1 gene-edited rabbit is obtained. The present invention successfully breeds a rabbit breed with increased weight; utilizing gene editing technology to develop new ideas for animal genetic breeding, has application value in promoting the improvement of rabbit genetic traits, enhancing rabbit production performance and economic value, and provides strong support for the high-quality development of my country's animal seed industry.