76 results about "Zostera marina" patented technology

The invention discloses a manufacturing method of paraffin sections of zostera marina embryo, comprising the following steps: (1) material drawing and fixation: zostera marina seeds are taken and the fruit skin is stripped, and after endosperm is removed, an integral zostera marina embryo is soaked in FAA (free amino acids) fixing solution and is fixed for more than 48 hours; (2) dehydration: the zostera marina embryo is taken out from the fixing solution and is soaked in alcohol for dehydration; (3) waxing and embedding: paraffin is added gradually in a vessel containing the zostera marina embryo and dimethylbenzene, and conventional paraffin embedding is carried out to obtain the wax blocks containing the zostera marina embryo; (4) slicing, spreading and drying: the wax blocks coated with the zostera marina embryo is fixed on a wheel rotation type slicing machine for carrying out continuous slicing of the paraffin, so as to obtain wax bands and enable the wax bands to be attached to an object slide; (5) dewaxing, rehydration and dyeing of hematoxylin dye solution; (6) slicing, dehydration and dyeing of eosin and alcohol; and (7) transparence and mounting are carried out to manufacture a permanent section. In the manufacturing method disclosed by the invention, the paraffin sections of the zostera marina embryo, which have clear dyeing and an integral texture structure, can be obtained.

The invention discloses a quick germination method of grass wrack seeds. The method comprises the following steps of: after the grass wrack seeds are washed clean, directly putting the grass wrack seeds into 0.5 percent low-salinity artificial seawater; putting the 0.5 percent low-salinity artificial seawater with the soaked grass wrack seeds into an incubator in the presence of constant temperature and illumination for culturing for 3 weeks to ensure that the grass wrack seeds germinate, wherein the holding temperature in the presence of the constant temperature and illumination is 12-16 DEG C, the illumination intensity is 40 mu molm<-2>s<-1> and the ratio of L:D is 12:12; leading the germinated seeds into a salinity raising and domestication stage: ensuring that the salinity of the artificial seawater for soaking the germinated grass wrack seeds is improved by 0.5 percent per day until reaching a normal seawater salinity of 3 percent; and then sowing the germinated grass wrack seedlings adapting to the natural seawater salinity in an appointed sea field. The method can ensure that the grass wrack seeds germinate quickly and completely and normally grow so that the probability of suffering from being swallowed by seed-eating organisms of the grass wrack seeds is greatly reduced and is beneficial to the recovery and reconstruction of sea grass meadows so as to quickly recover good marine ecological environment.

Aiming at solving the existing problems that an existing repairing method of a zostera marina seagrass bed is low and a germination rate of seeds is low, the invention provides a cultivation method of zostera marina artificial sea turfs. Seed propagation is used in the cultivation process, existing seagrass bed resources do not need to be damaged, and a group genetic diversity of a repairing area is increased. A seedling grows in a 'seedling base' in an earlier stage, substrate particle size is small, nutrition is rich, rhizomes of the seedling grows fast, a survive rate of the seedling is high, adaptability to a transplant sea area is strong, and the cultivation method of the zostera marina artificial sea turfs is applicable to a new-built seagrass bed and overcomes the shortcoming that direct seed sowing is low in germination rate.

The invention relates to the field of ecological restoration and reconstruction of seagrass meadows, in particular to a method and device for transplanting zostera marina L.. The device is provided with brackets and zostera marina L. fixing grids, wherein the zostera marina L. fixing grids are put in the brackets; and one or a plurality of zostera marina L. fixing grids are put in each bracket. The method comprises the following steps: (1) fixing the zostera marina L. on the zostera marina L. fixing grids; (2) after the zostera marina L. plants are fixed on the zostera marina L. fixing grids, vertically clamping grooves at the two ends of a main plate on two spaced cross beams of the brackets; and (3) utilizing the device to transplant seagrass in the sea area. The method and the device have the following beneficial effects: the problem that the seagrass in the sea area in the subtidal zone is difficult to transplant is solved, and in combination with the simple, convenient and effective device, restoration and reconstruction of the zostera marina L. seagrass meadows in the subtidal zone can be conveniently and rapidly carried out; and meanwhile, the device can be recycled, thus saving the cost, and the used strings are made of degradable plant materials, thus not polluting the sea area.

The invention relates to the field of seaweed life, physiological, and ecological habit study. The invention relates to a zostera marina indoor long-term cultivation method and a device for realizing the method. The cultivation device is composed of four parts which are an aquarium, a lighting system, a circulation filtration system and a water temperature regulation system. The cultivation method comprises the following steps: zostera marina is planted in a plastic pot with sediment mud sterilized by sunlight exposure; The pot is placed in the aquarium, and fresh sea water is added, wherein the water level of the sea water is higher by 10cm than the top of the zostera marina plant; a water circulation condition is twice/h, a lighting condition is 12h light/12h dark and 5000lux, a water temperature is 10-25 DEG C, 1/3 of the sea water is replaced every month, and a salinity is 3-3.7%; impurities on the inner wall of the aquarium are removed regularly; and the sediment mud is replaced according to plant growth conditions. If the physiological functions of zostera marina are to be studied, light intensity, water temperature and sea water salinity can be adjusted according to needs, and nutrients can be added to the small pot. The method and the device provided by the invention are suitable for the cultivation of zostera marina in scientific researches, such that life habits, physiological characteristics and ecological characteristics of zostera marina can be studied.

The invention discloses a fabrication method of seagrass seedling protoplast, comprising the following steps: temporarily raise the seagrass seedlings in double-anti sea water, select the seedlings that just sprout and sequentially put them into alcohol with the concentration of 75 percent, sodium hypochlorite solution with the concentration of 1 percent and potassium iodide solution with the concentration of 1.5 percent for disinfection, then wash, soak and restore the seedlings by sterile seawater. Then, the seagrass seedlings are shredded and placed into centrifugal tube with enzyme solution for enzymolysis. After the enzymolysis, glucose liquid is added before filtration. Wash the seedlings thrice with hypertonic sea water and obtain the precipitate after centrifugation. Add medium to the precipitate and obtain the protoplast through centrifugation. The invention can avoid pollution and obtain sterile seagrass seedling protoplast and has laid a solid foundation for the protoplast fusion, genetic transformation, plant regeneration as well as new breeds breeding. Moreover, the invention can also provide technical reserves for seagrass propagation of cell engineering, rapid propagation of fine lines seagrass and improving seagrass genetic engineering.

The invention relates to a new application of fucoidan sulfate in preparation of drug for preventing and treating chronic obstructive pulmonary disease (COPD). The fucoidan sulfate has fucose content greater than 29% and sulfate group content greater than 30%; and is a polysaccharide sulfate extracted from Phaeophyta algae such as Zostera Marina L., Laminaria Japonica Aresch., Sargassum Phyllocystum, Surgassum Thunbergii, Sargassum Integerrimum, Sargassum Fusiforme (Harv.) Setchel, Sargassum naozhouense, Sargassum Muticum, Sargassum Pallidum, etc. Animal tests show that fucoidan sulfate has definite curative effects in preventing and treating COPD, and relieving development of COPD.

The invention provides a preparation method of a sea cucumber bait. The preparation method solves the technical problems of instable quality, unreasonable nutritional composition, immature production process, less microbe content, not ideal effect, and the like of a raw material. The preparation method comprises the following steps of: inoculating lactic acid bacteria strains, microzyme strains, photosynthetic bacteria strains and bacillus stains to aquatic feed yeasts, float sludge and zostera marina powder in purified seawater, wherein the balance is the purified seawater, and the content is in terms of weight percent; carrying out liquid-state fermentation on a mixture at room temperature; and adding vitamins, immune polysaccharide, an enzyme preparation, amino acid peptides and radix astragali polysaccharide to a product to prepare the sea cucumber bait. The preparation method provided by the invention can be used for preparing the sea cucumber bait.

The invention relates to a method for extracting a flavonoids compound from zostera marina. According to the method provided by the invention, the zostera marina is taken as a raw material, the extracted flavonoids compound and metal salt are enabled to be in reaction, stirring is performed, temperature and pH value are adjusted, after the reaction, precipitates are collected and dissolved into an ethanol solution, then an EDTA decomplexing mixture is added for oscillating reaction, rotating and drying by distillation are performed, then methyl alcohol is added for dissolving and filtering, and then filtrate concentration and drying are performed, namely the flavonoids compound of the zostera marina can be obtained after purification. The method provided by the invention can enable the content of the flavonoids compound of the zostera marina to reach more than 55 percent. According to the method provided by the invention, the technological process is simple, the operation period is short, the raw material is easy to access, solvent recovery is easy, energy consumption is low, and the method is a purification method for the flavonoids compound and has broad prospects for development.

The invention relates to sea grass bed restoration and reconstruction technologies, in particular to a subtidal zone eel grass sowing and planting method. The method includes: using clay and fine sand to make a mud box, well mixing the seeds of eel grass with appropriate amount of fine sand and placing the mixture into the inner cavity of the mud box, sealing the opening of the mud box, throwing the mud box filled with the seeds of the eel grass into the sea of appropriate sea areas so as to perform eel grass bed ecological restoration, wherein the sowing density of the seeds of the eel grass is 1-1.5 kilograms per mu. By the eel grass sowing method simple and efficient, the problem that sea grass sowing at seabed of deep subtidal zone water areas is difficult is solved, eel grass bed restoration or reconstruction of deep subtidal zone water areas can be performed conveniently and fast, and the materials used by the method cause no sea area pollution.

To solve the problem that distortion can be easily caused in a seed germination process when an existing low salt stimulus method is used to cultivate zostera marina, the invention provides a cultivation method of zostera marina high-quality seedling plants. According to different development stages in the germination process of seeds of the zostera marina, different cultivation conditions are procedurally regulated, invade and harass of mildew and mussy algae in the seed germination process can be reduced, and the shortcoming that the distortion can be easily caused in the seed germination process by using of traditional seedling plant obtaining method is overcome to a certain degree. The zostera marina is cultivated by adoption of the method, germination time of the seeds of the zostera marina can be shortened, a germination rate can be improved by 30%-40%, a growing length of cotyledons can be remarkably increased by 2-3 times, a distortion rate of the seeds in the germination process is controlled within 2%, the cultivated cotyledons of seedlings are thick and solid and outstretched, plants are robust, and a growth rate is quick.

The invention relates to a method for collecting zostera marina seeds. The method comprises collection and processing of zostera marina reproduction branches, temporary rearing of the zostera marina reproduction branches and harvest and saving of the zostera marina seeds. The collection and processing of zostera marina reproduction branches comprises collecting the zostera marina reproduction branches and removing erect stems of the reproduction branches; the temporary rearing of the zostera marina reproduction branches comprises filling the zostera marina reproduction branches in a mesh bag, sealing, strapping, and tying on a rope with a buoy at one end and a sinker at the other end, and placing in a natural sea area for temporary rearing; and the harvest and saving of the zostera marina seeds comprises temporarily rearing the zostera marina reproduction branches for certain time, taking back, pouring in a container, stirring, stewing, filtering, washing and saving. The method can collect the zostera marina seeds in a large scale, can take full advantages of conditions such as seawater, illumination, nourishment and temperature of natural sea area, is low in cost, lightens labor strength of seed collection, and is high in seed collection efficiency, good in seed quality and small in influences on original ecology meadows.

The invention relates to a natural sea-area sowing method of zostera marina seed, and the method comprises zostera marina seed sowing time, zostera marina seed pretreatment and zostera marina seed sowing. The zostera marina seed is sowed at later autumn. The zostera marina seed pretreatment steps comprise performing immersion disinfection on the zostera marina seed respectively by ethyl alcohol and HgCl2, flushing with aseptic seawater, putting in low-salt aseptic seawater, putting in a dark environment with proper temperature for pre-treating for a period. The zostera marina seed sowing steps comprise uniformly mixing pretreated zostera marina seed with bottom mud or silt, packing into a mesh bag, sealing with a cotton thread, flatly paving seabed, and mutually connecting and fixing the mesh bags by U-shaped iron wires, so as to form a flat-paved carpet type sowing unit. The method is capable of effectively improving the germination rate of the seed, also has extremely good promoting effect on growth of germinated seedlings and plants. By employing the mesh bag for sowing during sowing, complete reserving of the sowed zostera marina seed is guaranteed, and thus the utilization rate of the seed is improved.

The invention relates to a zostera marina seed multiplication method and belongs to the technical field of ocean resource recovery. The method particularly includes the following steps: (1), collecting zostera marine seeds, storing the seeds into fresh seawater 32psu after surface disinfection with alcohol of 70%, performing inflation saving at 17-20 DEG C, and saving in a refrigerator for 30 days at 4 DEG C before seeding; (2), starting to seed in the middle of November; (3), transplanting to a sea area in June next year, wherein the seeding method particularly includes 1, selecting a shrimp pond with water depth of 40-100cm and bottom sediment to be a sand-mud bottom; 2, making a transplanting frame; 3, seeding; 4, cultivating in the shrimp pond. During sea area transplanting, an area with water depth of subtidal zone to be 20cm, bottom sediment to be a sand-mud bottom and sand-mud proportion to be 1:3-1:5 is selected, and tidewater needs to have certain flow speed, 6cm/s properly. By the zostera marina seed multiplication method, damage to existing resources is avoided, zostera marina multiplication can be effectively performed, and effect of restoring zostera marine resource quantity is realized.

The invention relates to a wild harvesting method of Zostera marina L. seeds. The method comprises the following steps: at a low-tide stage when peels on first spathe inflorescences of most flower lateral branches are initially cracked, harvesting flowering branches, putting the harvested flowering branches into 20-mesh bolting silk string bags till each string bag is filled by a half, tightening the mouths of the string bags, connecting the plurality of string bags together, and locally putting the connected string bags back into sea water; 15 days later, taking the string bags out and cleaning substances attached to the surfaces of the string bags; about 30 days later, taking the string bags out, locally pouring the flowering branches in the string bags into a plastic water bucket, adding sea water, stirring vigorously, and picking out residual flowering branches on an upper layer after seeds fall to the bottom of the bucket; adding sea water repeatedly, stirring, and pouring out immature seeds floating on the upper layer; and filtering large shellfishes out by using a 10-mesh mesh sieve in sea water, filtering small shellfishes and residues out by using a 30-mesh mesh sieve, and selecting plump Zostera marina L. seeds. By using the wild harvesting method, a high seed harvesting rate is ensured under the condition of low cost, and damages to populations caused by harvesting of the flowering branches are reduced to the maximum extent. The wild harvesting method can be applied to any Zostera marina L. growing sea area.

The present invention relates to a sea cucumber compound feed, especially to a compound feed suitable for stichopus variegatus growth and ripening. According to the stichopus variegatus food composition characteristics, the compound feed adds a high proportion of oyster shell powder as a main raw material for producing the stichopus variegatus compound feed. The compound feed includes the following components (in weight percentage): 10% sargassum powder, 10% sargassum thunbergii powder, 10% zostera marina powder, 10% soybean protein, 40% oyster shell powder, 5% fish meal, 10% sea mud, 1% shrimp brans, 0.5% yeast powder, 0.5% immune polysaccharides, 1% compound vitamins, 1% inorganic salt mixtures and 1% adhesive. All the raw materials are grinded using a grinder, screened using a 100 mesh sieve, thoroughly mixed, added with an appropriate water, made into granules using a granulator, and dryed into a granular feed with a length of 1-2 cm and a diameter of 3-4 mm. The feed is non-polluting and non-residual, and has non-toxic side effects. Compared with the traditional sea cucumber feed, the increased proportion of oyster shell powder in the feed composition can partly replace seaweed powder and reduce production costs. The stichopus variegatus has a better adaptability to the compound feed and can have a good growth and ripening after fed by the compound feed.

The invention relates to a zostera marina young seedling manual culture method, which comprises the steps of water temperature setting, salinity setting, illumination setting, substrate setting, dissolved oxygen content setting and water circulation setting during zostera marina young seedling manual culture. During the water temperature setting, the water temperature is controlled to be two gradients, and the water temperature in a young seeding culture initial stage is lower than that in a second stage; during the salinity setting, the water body salinity is controlled to be a in low-salinity environment; during the illumination setting, the illumination time cannot be too short every day, and the photo flux density is proper; during the substrate setting, the pavement thickness is proper, and the substrate is fine sand with the prior particle diameter; during the dissolved oxygen setting, a water body is continuously oxygenated for preventing the too low dissolved oxygen content; during the water circulation setting, the water body continuously circulates, and the circulation speed is prevented from being too higher. The method obtained in the way has the advantages that the large-scale manual culture of zostera marina young seedlings can be realized, and the growth effect of the zostera marina young seedlings can also be obviously improved.

The invention relates to a method for cultivating zostera marina in coastal waters. The method is characterized in that a substrate is added in collection boxes, and the collection boxes with the substrate are put in a pond; zostera marina in the pond grows naturally and creped into the collection boxes; before zostera marina seed maturity, the collection boxes are moved out of the pond, and are put in coastal waters at determined positions; the seeds are naturally spread by the zostera marina in the collection boxes, and a zostera marina spreading propagation process is finished.

The invention discloses an ecological wind power pile foundation bottom protection structure and a design method, and belongs to the technical field of pile foundation bottom protection. The design method is characterized by comprising the steps that S1, basic data are obtained, and the basic data comprise the wave height H, the wavelength L, the water depth D and the foundation width d of a wind power pile foundation in the 0-10 m coastal near shore; S2, the relationship among the bottom flow velocity, the water depth and the block stone mass is obtained, the bottom flow velocity V is calculated according to the wave height H, the wavelength L and the water depth D; the mass W of a stabilizing block stone at different bottom flow velocities is calculated, and fitting is carried out on the relationship among the bottom flow velocity V, the water depth D and the block stone mass W; S3, a block stone protection range is determined; S4, the space between the block stones is filled with graded stones; S5, zostera marina is fixed to the graded stone; and S6, a reinforcing net is laid on the block stones, and the periphery of the reinforcing net is fixed. According to the technical scheme of the ecological wind power pile foundation bottom protection structure and the design method, the wind power pile can be prevented from being scoured by sea waves, meanwhile, the ecological wind power pile foundation bottom protection structure has the advantages of being ecological and environmentally friendly, and water quality can be improved and the ecological environment can be restored while scour is prevented.

The present invention relates to a zostera marina somatic embryo induction method, which comprises that the spadix of zostera marina is adopted as an explant, the inflorescence being subjected to a surface disinfection treatment is cut into fragments with a length of 3-5 mm, the fragment is inoculated into a somatic embryo induction culture medium, induction culture is performed at a temperature 15-25 DEG C under a dark or weak illumination condition, transfer inoculation is performed every two weeks, tissue sprouting can be generated on the inflorescence after culture for 15-25 d, small enation is generated to gradually develop into the somatic embryo, and during the process, the callus stage does not exist, wherein the somatic embryo induction culture medium is a solid culture medium adopting a MS culture medium and a N6 culture medium as a base culture medium and added with an organic additive, a carbon source, plant hormones (auxin or auxin and cytokinin), and sea salt. According to the present invention, the callus induction culture stage does not exist, and the zostera marina somatic embryo is directly induced from the inflorescence tissue within the short time, such that the important technical support is provided for the zostera marina sterile tissue culture seedling regeneration and the tissue culture rapid propagation system establishing, and the foundation is established for the successful seaweed tissue culture.

The invention relates to the technical field of fish shelters, and particularly discloses a fish reef structure group. The fish reef structure group comprises at least four fish reef structures, and each fish reef structure comprises steel framework fish reefs, stone fish reefs and concrete fish reefs, wherein the steel framework fish reefs are distributed on the periphery of the fish reef structure, the stone fish reefs and the concrete fish reefs are arranged in the fish reef structure, the transverse distance between every two adjacent fish reef structures is 100 meters, the longitudinal distance between every two adjacent fish reef structures is 200 meters, and sargassum thunbergii, gulfweed and zostera marina are cultivated on the peripheries of the steel framework fish reefs, the stone fish reefs and the concrete fish reefs. The fish reef structure group has the advantages that the fish reef structures are arranged and integrally form the fish reef structure group, and therefore proliferation rates of fishery resources are greatly improved; the cultivation of peripheral seaweed facilitates growth and cultivation of seafood treasure; the effect that different fishes are well induced and collected by fish reef piled body combinations with different textures and specifications can be generated.

The present invention relates to a zostera marina callus induction method, which comprises zostera marina inflorescence is adopted as an explant, the surface-sterilized inflorescence is cut into the material with a length of 3-5 mm, the obtained material is inoculated into a callus induction culture medium, callus induction is performed, transfer inoculation is performed every two weeks, both sides of the floral axis expand after culture for near 1 month, and the callus is gradually formed, wherein the callus induction culture medium adopts an improved MS culture medium as a base culture medium and is added with 2,4-D, casein hydrolyzate, sucrose, agar and sea salt. According to the present invention, the improved MS culture medium is used, such that the zostera marina callus induction can be effectively promoted, and the important technical support is provided for the production and the rapid propagation of the zostera marina tissue culture regeneration seedlings.

The invention provides a sowing method of zostera marina seeds. The sowing method comprises the following steps: firstly, mixing the zostera marina seeds, stones and nitrogen fertilizers; then adhering the materials by glutinous rice flour; drying the mixture; and then sowing the mixture in a sea area. According to the method, the zostera marina seeds are placed in a slow-release system, so that certain weight is added to the zostera marina seeds which then can fall into a sowing area. In addition, components in the slow-release system can be provided for germination and growth of the zostera marina seeds, thus guaranteeing the survival rate of zostera marina.