Method for biologically purifying aquaculture water body
A technology of biological purification and mariculture, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc.
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Embodiment 1
[0017] The cultivation of embodiment 1 bacillus
[0018] 1. Bacillus strains are isolated from the breeding sewage, and the conventional nutrient agar medium and nutrient broth medium are prepared.
[0019] 2. Culture of bacteria
[0020] Inoculate the bacillus strains on the slant of nutrient agar medium under aseptic conditions, and then culture them in an incubator at 37°C for 24 hours, and a layer of white round bacillus colonies with smooth edges can be seen. Aseptically inoculate the bacillus cultured in the nutrient agar medium into 100ml nutrient broth medium, shake well, and cultivate in a shaker at 37°C for 2-3 days, and set aside.
Embodiment 2
[0021] The cultivation of embodiment 2 nitrifying bacteria and nitrosifying bacteria
[0022] 1. The strains of nitrifying bacteria and nitrosifying bacteria are separated from the breeding sewage, and the conventional liquid culture medium of nitrifying bacteria and nitrosifying bacteria is prepared.
[0023] 2. Culture of bacteria
[0024] Inoculate the nitrifying bacteria and nitrosifying bacteria preliminarily separated from the aquaculture sewage into a 100ml test tube in a shaker at 20-28°C at 200 rpm, shake and culture for 3-5 days, and set aside.
Embodiment 3
[0025] The cultivation of embodiment 3 photosynthetic bacteria
[0026] 1. The photosynthetic bacteria strains are isolated from the breeding sewage, and the conventional photosynthetic bacteria solid and liquid culture medium are prepared.
[0027] 2. Culture of bacteria
[0028] Inoculate the photosynthetic bacteria strains on the photosynthetic bacteria solid medium under aseptic conditions, and then culture them in an incubator at 30°C for 24 hours under anaerobic conditions, and make the photosynthetic bacteria cultured on the photosynthetic bacteria solid medium sterile Inoculate into 100ml of photosynthetic bacteria liquid medium, culture at 30°C for 3-5 days under anaerobic conditions, and set aside.
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