96 results about "Nutrient broth" patented technology

The invention discloses microbial feed additive used for pigs, and a preparation process thereof; the prescription is as follows: 1 portion of bacillus licheniformis, 1 portion of bacillus subtilis, 2 portions of bifidobacterium, 2 portions of enterococcus faecalis, 2 portions of enterococcus faecium, 1 portion of boas-oppler bacillus, 1 portion of lactobacillus casei, 1 portion of lactobacillus lactis, 1 portion of plant lactobacillus, 1 portion of candida utilis, 1 portion of saccharomyces cerevisiae, 80kg to 100kg of panela, 3kg to 5kg of salt, 5kg to 8kg of vinegar, 3kg to 5kg of lysine, 1kg to 2kg of methionine, and 1000L of pure water; the weight of each portion of strain is 2g to 5g. The preparation process is as follows: strain preparation, nutrient broth preparation, inoculated fermentation and check. The invention reduces the quantity of the used individual strain, and the strain quality is safe and reliable. The production process is simple and feasible, the effect is obvious; therefore, the invention has promotion value.

The invention relates to a method for solidifying sand by utilizing halotolerant bacteria. The method selects Sporosarcina pasteurii as bacteria, the strain comes from American Type Culture Collection (ATCC), and the collection number is ATCC 11859. The method comprises the following steps: putting the strain in a nutrient broth medium to obtain a bacterial solution after domestication and enrichment; putting the bacterial solution in desert underground brine containing urea, calcium ions and salinity, and culturing to obtain a bacterial solution with an induced solidification effect; spraying the bacterial solution on the surface of sand soil; putting the sand soil of which the surface is sprayed with the bacterial solution in the natural drying environment for culturing; and naturally air-drying, so that the bacteria can utilize the desert underground brine to cement and solidify sand grains on the surface of the sand soil through the solidification effect so as to prevent the sand soil on the surface layer from migrating under the wind effect. The method is environment-friendly, is low in cost, and can be widely used for solidifying mobile and semi-mobile dunes.

The invention belongs to the technical field of microbial functional inoculants, and specifically relates to an inoculant for degrading aflatoxin B1 and applications thereof. The inoculant is prepared through amplification culture of brevibacterium halotolerans JH8 in nutrient broth. The amplification culture is performed in a shaking table incubator, the culture conditions are: the rotation speed is 150 to 170 r/min, the temperature is 30 to 33 DEG C, and the culture time is 18 to 24 hours. The provided inoculant can degrade aflatoxin B1 in grains until no aflatoxin B1 is detected by a kit. The brevibacterium halotolerans JH8 is cheap and easily available. The preparation method is simple and easy to operate. Moreover, the inoculant is pollution-free to the grains.

The embodiment of the invention relates to a preparation method of freeze-dried pellets for quantitatively preserving microorganisms. The preparation method comprises the following steps: inoculating target strains through nutrient agar and increasing bacteria of the target strains through single-colony nutrient broth, then obtaining a microorganism bacterium liquid of a later period of a logarithmic growth period; diluting the microorganism bacterium liquid and controlling bacterium concentration through assistant of ultraviolet light absorption to obtain a uniform bacterium suspension with a quantitative concentration; mixing a protection agent with bacterium-free water to prepare a protection agent solution; mixing the bacterium suspension with the protection agent solution to obtain a mixed solution, wherein the bacterium concentration of the mixed solution is 102cfu/50microliters-106cfu/50microliters; taking a quantitative amount of the mixed solution, freezing the mixed solution into pellets by using liquid nitrogen and then rapidly pre-freezing, wherein the pre-freezing time is not shorter than 2 hours, and the pre-freezing temperature is between 80 DEG C below zero and 20 DEG C below zero; and freeze-drying the pre-frozen pellets in vacuum to obtain freeze-dried pellets.

The invention discloses a Burkholderia sp. Strain ES-89 for preventing and treating fungal diseases of plants as well as a culture method and application. The culture method comprises the following steps: A, inoculating an ES-89 strain into a nutrient broth culture liquid for activation; B, inoculating the activated ES-89 into a triangular flask with the nutrient broth culture liquid, and performing shake cultivation; C, centrifuging fermentation broth obtained from shake cultivation under a certain condition, and filtering an upper layer of fermentation broth by a bacterial filter to obtain sterile supernate; D, washing the bacterium with sterile water to prepare a bacterial suspension, and storing the fermentation broth, the fermentation supernate and the bacterial suspension. The strain is preserved with the preservation number of CCTCC, CCTCC NO: M 2016665, and the strain ES-89 can be applied to preparation of medicines for treating or preventing plant pathogenic fungi. The strain has an inhibition function on coptis chinensis leaf spot diseases and the like, and can be applied to prevention and treatment on multiple plant diseases. The prevention and treatment effect of the strain on the coptis chinensis leaf spot diseases can be 88% or greater, the culture method is feasible, the operation is simple and convenient, the pollution in the production process is reduced, solvent poisoning is avoided, and the application prospect is relatively good.

The invention discloses a microbial reinforcement liquid for slope reinforcement, a preparation method and a construction method thereof, and relates to a microbial reinforcement liquid. The adhesivebinder is a sodium ion solution, strains of the mineralized bacteria solution A are bacillus subtilis and bacillus licheniformis, the OD600 value is 1.0-1.5, the mineralized bacteria solution A comprises distilled water, 3-5g/L of nutrient broth, 35-45g/L of urea, 65-85g/L of calcium chloride and 10-20g/L of ammonium chloride, and strains of the mineralized bacteria solution B are sporosarcina pasteurii and rhodopseudomonas; the OD600 value ranges from 0.5 to 1.0; the mineralized bacteria nutrient solution B comprises distilled water, 4-6g/L of nutrient broth, 25-35g/L of urea, 45-65g/L of calcium chloride and 10-20g/L of ammonium chloride. The mineralization ability of bacillus subtilis and sporosarcina pasteurii is utilized to carry out ecological reinforcement on the expansive soil slope.

The invention relates to a preparation method of a compound microbial flocculant for cyanobacterial bloom. The preparation method comprises the following steps: respectively inoculating Serratia and Bacillus into a sterilized nutrient broth medium for culture; respectively taking out 1mL of bacterial liquids of Serratia and Bacillus after culturing for 24h, and inoculating into a sterilized fermentation medium for fermentation culture; treating a broth as a seed liquid after culturing for 72 h and carrying out complex culture for 72h under conditions of a culture temperature of 35 DEG C, a rotation speed of 160rpm, an initial pH of a medium of 8.0, and an inoculation amount of the seed liquid of 10 ml/L; and carrying out centrifugal separation on the broth, and taking out a supernatant liquor. So the compound microbial flocculant is obtained. The preparation method is simple, fast and low cost, the flocculant prepared in the invention has the advantages of small dosage in use, strong stability and easy storage, can effectively flocculate cyanobactreial water samples with a flocculation rate of 92%, and has an excellent application prospect.

The invention discloses a preparation method of a Cynoglossus semilaevis vibrio anguillarum vaccine, which is characterized in that Cynoglossus semilaevis pathogeny Listonella anguillarum BH1 serves as an immunogen, and Astragalus mongholicus lixivium serves as an adjuvant. The preparation method comprises the following steps: inoculating the Cynoglossus semilaevis pathogeny Listonella anguillarum BH1 bacterial strain on a common nutrient broth culture medium; after the bacterial strain is cultured, inactivating by formaldehyde to obtain inactivated bacteria; after dipping the Astragalus mongholicus with distilled water, decocting, and filtering; condensing until the final concentration is 1g of raw drug per ml, and obtaining the Astragalus mongholicus lixivium; and mixing the inactivatedbacteria with the Astragalus mongholicus lixivium to obtain the Cynoglossus semilaevis Vibrio anguillarum vaccine. When the vaccine prepared with the method disclosed by the invention is used for immunizing Cynoglossus semilaevis, the capability of the Cynoglossus semilaevis on resisting Vibrio anguillarum infection can be obviously improved, Vibrio anguillarum infection during the culture production of the Cynoglossus semilaevis can be effectively prevented, the death rate of the Cynoglossus semilaevis is reduced, the culture yield of the Cynoglossus semilaevis is improved, the Cynoglossus semilaevis culture production is guaranteed to healthily and sustainably develop, and the economic benefit for Cynoglossus semilaevis culture production is improved.

The invention provides a method for counting the effective viable bacterium content of Bdellovibrio bacteriovorus powder, which comprises the following steps: appropriately diluting a sample to be detected, so that Bdellovibrio bacteriovorus in the sample to be detected is sufficiently dispersed into individual cells; then, carrying out incubation culture in an aseptic nutrient broth diluted by 10 times to promote growth and propagation, thus forming a megascopic turbid liquid; further determining that a large amount of Bdellovibrio bacteriovorus is contained in the turbid liquid having the maximum dilution through plaque detection; and finally, determining the total amount of effective viable bacteria in the sample to be detected. Compared with a microscopic direct observation method anda dilution-by-tap-water double-layer agar plate method, the method provided by the invention is more accurate, and overcomes the defects that detection is inaccurate due to dilution in the dilution-by-tap-water double-layer agar plate method and killed bacteria and viable bacteria can not be distinguished in the microscopic direct observation method.

An insoluble phosphate bioleaching method is characterized in that insoluble phosphate raw materials are crushed and sieved by a 2mm sieve, bacillus is qualitatively and quantitatively measured by a phosphate dissolving ring and a liquid shake flask culture medium, efficient strains with high phosphate dissolving capacity is screened, 2-8ml screened efficient strains are inoculated into 100ml of nutrient broth media and cultured for 3-5 days under the condition of 25-35 DEG C and 100-150r/min to serve as bacteria, 10% of sucrose is added into clear water and serves as a nutrient source, 1-7% of insoluble phosphate raw materials are added, 2-8% of the cultured bacteria are inoculated and cultured for 7-10 days under the condition of 25-35 DEG C and 120-160r/min, and solid residues and culture solution are separated until the content of water-soluble phosphorus in the culture solution is higher than or equal to 100mg/L. The selected bacteria have an efficient phosphate dissolving function and are high in phosphate bioleaching rate, the content of beneficial microorganisms in soil can be increased, and phosphorus use rate is increased.

The invention relates to piglet pellet feed preparation method which comprises the following steps: (1) micro pulverization and even mixing are conducted to complete feed through a conventional feed processing method; (2) the mixed complete feed micro powder is poured into an inclined rotary disk granulating machine, and vaporous adhesive nutrient broth is evenly sprayed on the complete feed micro powder in the disk granulating machine, to prepare spherical granular feed; (3) the spherical granular feed is spread on a mesh-belt drying machine, to be heated and dried; (4) the granular feed is cooled through a counterflow cooling method for conventional feed processing; the particles of the granular feed prepared by the method are more floppy and crispy; the method improves the palatability of piglets, meanwhile, improves the granulation efficiency, reduces the granulation energy consumption and saves the artificial cost of granulation.

The invention discloses a method for extracting active polysaccharide of wheat bran. The method comprises seed liquid preparation, fermentation medium preparation, fermentation and active polysaccharide extraction and concretely comprises inoculating nutrient broth with revived schizophyllum commune, bacillus subtilis, lactic acid bacteria and saccharomyces cerevisiae according to a weight ratio,carrying out culture to obtain a seed liquid, mixing wheat bran, soybean meal, corn core powder, distilled water and a fermentation synergist to obtain a fermentation medium, inoculating a fermentation medium with the seed liquid, carrying out fermentation culture, and extracting the fermented wheat bran through distilled water and an ethanol solution to obtain an active polysaccharide solution. The fermentation synergist can greatly increase the yield of the active polysaccharide. The method can significantly reduce the probability that the wheat bran active polysaccharide is separated alongwith the protein denatured gel and increase the yield and content of the wheat bran active polysaccharide. The wheat bran active polysaccharide can be used as an antioxidant active ingredient to remove free radicals in the body.

The invention relates to a method for treating mercury in water with escherichia coli. According to the method, escherichia coli is used and cultured in an enrichment mode in a nutrient broth medium to a stable growth period on the anaerobic condition, then the water containing divalent mercury is inoculated with escherichia coli, nutrient broth serves as nutrition to maintain bacterium growth, and anaerobic culture is carried out for several days on the room temperature condition to stop the experiment. The divalent mercury ions (Hg2+) in the sewage can be converted into mercuric chloride (HgCl) precipitate by escherichia coli, and mercuric chloride (HgCl) precipitate is difficult to dissolve in water, can stably exist in a water body and finally deposits to the bottom of the water body, so that divalent mercury pollution in the water body is removed, harm to the human body is reduced, and secondary pollution is avoided. The method has the advantages of being low in cost, wide in application range and the like.

The present invention discloses primer sequence and detecting reagent kit for detecting delayed Edward's bacteria. The detecting reagent kit contains the following reagents: mixture of nutrient broth and aged sea water through autoclaving; mixture of PCR buffer, Taq DNA polymerase and sterile double distilled water; mixture of nucleotide sequence of SEQ ID No. 1 and SEQ ID No. 2, dNTPs and MgCl2; genome total DNA of delayed Edward's bacteria; sterile double distilled water; TBE electrophoretic buffer containing Tris-boric acid and EDTA; and agarose containing ethidium bromide. The present invention has primer sequence with optimized PCR condition and system, and the PCR detecting method for detecting delayed Edward's bacteria and the detecting reagent kit are simple, fast, sensitive and specific.

The invention relates to an application of bacillus subtilis in antagonism helicobacter pylori, wherein substances capable of restraining the growth of helicobacter pylori can be generated when the shake cultivation is performed on the bacillus subtilis for 9 hours at 37 DEG C and at a speed of 180 rpm (revolutions per minute) in a nutrient broth liquid culture medium, thereby efficiently restraining the growth of the helicobacter pylori; when the bacillus subtilis is cultivated for 18 hours, the antibiotic ability is maximum; and antagonistic proteins are acquired by purifying the substances, thereby preparing a preparation for treating gastrointestinal tract diseases. The invention has the following technical effects: the bacillus subtilis from probiotics has a function of maintaining the micro ecological balance of an intestinal tract; the bacillus subtilis culture has stronger antagonism to the helicobacter pylori; and compared with the antibiotic treatment method used for conventionally treating the helicobacter pylori, the bacillus subtilis can be used for overcoming the defects that the side effect by using antibiotic treatment method is strong and the symptoms are easily reappeared after the disease is treated, and the like.