96 results about "Nutrient broth" patented technology

The invention discloses microbial feed additive used for pigs, and a preparation process thereof; the prescription is as follows: 1 portion of bacillus licheniformis, 1 portion of bacillus subtilis, 2 portions of bifidobacterium, 2 portions of enterococcus faecalis, 2 portions of enterococcus faecium, 1 portion of boas-oppler bacillus, 1 portion of lactobacillus casei, 1 portion of lactobacillus lactis, 1 portion of plant lactobacillus, 1 portion of candida utilis, 1 portion of saccharomyces cerevisiae, 80kg to 100kg of panela, 3kg to 5kg of salt, 5kg to 8kg of vinegar, 3kg to 5kg of lysine, 1kg to 2kg of methionine, and 1000L of pure water; the weight of each portion of strain is 2g to 5g. The preparation process is as follows: strain preparation, nutrient broth preparation, inoculated fermentation and check. The invention reduces the quantity of the used individual strain, and the strain quality is safe and reliable. The production process is simple and feasible, the effect is obvious; therefore, the invention has promotion value.

The invention provides a preparation method and application of a sea bdellovibrio bacteriovorus ecological preparation, and relates to a sea microbial ecological preparation and a preparation method of a bdellovibrio bacteriovorus preparation for mariculture. The preparation method comprises the following steps of: preparing a host bacteria suspension; separating and purifying bdellovibrio bacteriovorus; and propagating and culturing bdellovibrio bacteriovorus. The preparation method is characterized in that after pseudomonas stutzeri is connected with an incline and is activated, adding physiological saline in a test tube to prepare the bacterial suspension; then propagating and culturing with nutrient bouillon; adding a flocculating agent in the obtained bacterial suspension; standing and precipitating after uniformly shaking; adding the physiological saline to obtain 1012 cfu / mL of host bacteria suspension; separating and purifying the bdellovibrio bacteriovorus of collected sea and bottom sediment samples with a double agar plate method; pouring culture media containing the host bacteria suspension and the collected samples into sea agar in the lower layer for culture after uniformly mixing; and adding the host bacteria and the bdellovibrio bacteriovorus obtained by separation into sterilized natural sea, and culturing until the concentration of the bdellovibrio bacteriovorus is 108 to 109 pfu / mL for future use.

The invention relates to a method for solidifying sand by utilizing halotolerant bacteria. The method selects Sporosarcina pasteurii as bacteria, the strain comes from American Type Culture Collection (ATCC), and the collection number is ATCC 11859. The method comprises the following steps: putting the strain in a nutrient broth medium to obtain a bacterial solution after domestication and enrichment; putting the bacterial solution in desert underground brine containing urea, calcium ions and salinity, and culturing to obtain a bacterial solution with an induced solidification effect; spraying the bacterial solution on the surface of sand soil; putting the sand soil of which the surface is sprayed with the bacterial solution in the natural drying environment for culturing; and naturally air-drying, so that the bacteria can utilize the desert underground brine to cement and solidify sand grains on the surface of the sand soil through the solidification effect so as to prevent the sand soil on the surface layer from migrating under the wind effect. The method is environment-friendly, is low in cost, and can be widely used for solidifying mobile and semi-mobile dunes.

The invention relates to the restoration field of phenylarsonic acid pollutants, and concretely relates to a preparation method of load type biological iron-manganese composite oxide. The method mainly comprises the following steps: preparation of a nutrient broth medium, culture of flora, preparation of a biological manganese-iron medium, preparation of the load type biological iron-manganese composite oxide, and the like; Pseudomonas putida employs carbonate ore as an electron donor, a layered biological iron-manganese composite oxide is formed on the surface of manganese carbonate, and the load type biological iron-manganese composite oxide is obtained. The product can be used for fixing phenylarsonic acid compounds in soil and secondary products, such as arsenite and arsenate on the surface of mineral at the same time, in order to realize in-situ or non-in-situ restoration of phenylarsonic acid pollutants; the method is mainly suitable for soil with surface layer pollution, and the method has the advantages of simple operation, low treatment cost, and good effects without secondary pollution.

A composition comprising lactic acid bacteria that are selected from at least one of Lactococcus lactis, Lactobacillus lactis, and Lactobacillus brevis. The composition is suitable for administration to animals. Upon administration to an animal, the composition provides at least one of the following (a) improves performance in the animal and (b) reduces scours in animals. Also provided is a method of treating an animal in which the composition is administered to the animal. A method of forming a direct-fed microbial is also provided. A culture including the lactic acid bacteria is grown in a liquid nutrient broth. The lactic acid bacteria are separated from the liquid nutrient broth. The lactic acid bacteria can be freeze dried.

The invention relates to concentrated nutrient solution, nutrient broth and production methods thereof. The concentrated nutrient solution consists of the following raw materials in parts by weight: 26.5-30.5 parts of sea cucumber intestines, 69.5-73.5 parts of pure water, 0.05-0.1 part of protamex and 0.05-0.1 part of flavorzyme; the nutrient broth comprising the concentrated nutrient solution as a main raw material comprises the following raw materials in parts by weight: 99 parts of concentrated nutrient solution, 0.5 part of common salt, 0.25 part of hydrolyzed vegetable protein, 0.15 part of yeast extract and 0.1 part of monosodium glutamate; after scientific extraction, a sea cucumber mucopolysaccharide and sea cucumber saponin concentrated nutrient solution product of high added value is obtained, so that the utilization rate and the added value of the sea cucumber intestines are improved, and resource waste is avoided; and health-care products such as oral liquid, capsules and the like can be continuously developed by utilizing the product provided by the invention as a main raw material, particularly large price advantages can be achieved in comparison with the sea cucumber mucopolysaccharide and sea cucumber saponin product extracted by taking the sea cucumber as the main raw material, and the production cost is reduced.

The invention belongs to the technical field of microbial functional inoculants, and specifically relates to an inoculant for degrading aflatoxin B1 and applications thereof. The inoculant is prepared through amplification culture of brevibacterium halotolerans JH8 in nutrient broth. The amplification culture is performed in a shaking table incubator, the culture conditions are: the rotation speed is 150 to 170 r / min, the temperature is 30 to 33 DEG C, and the culture time is 18 to 24 hours. The provided inoculant can degrade aflatoxin B1 in grains until no aflatoxin B1 is detected by a kit. The brevibacterium halotolerans JH8 is cheap and easily available. The preparation method is simple and easy to operate. Moreover, the inoculant is pollution-free to the grains.

The embodiment of the invention relates to a preparation method of freeze-dried pellets for quantitatively preserving microorganisms. The preparation method comprises the following steps: inoculating target strains through nutrient agar and increasing bacteria of the target strains through single-colony nutrient broth, then obtaining a microorganism bacterium liquid of a later period of a logarithmic growth period; diluting the microorganism bacterium liquid and controlling bacterium concentration through assistant of ultraviolet light absorption to obtain a uniform bacterium suspension with a quantitative concentration; mixing a protection agent with bacterium-free water to prepare a protection agent solution; mixing the bacterium suspension with the protection agent solution to obtain a mixed solution, wherein the bacterium concentration of the mixed solution is 102cfu / 50microliters-106cfu / 50microliters; taking a quantitative amount of the mixed solution, freezing the mixed solution into pellets by using liquid nitrogen and then rapidly pre-freezing, wherein the pre-freezing time is not shorter than 2 hours, and the pre-freezing temperature is between 80 DEG C below zero and 20 DEG C below zero; and freeze-drying the pre-frozen pellets in vacuum to obtain freeze-dried pellets.

The invention relates to a C and D type C.perfringens antitoxin serum and a preparation method thereof. A C type C.perfringens strain and a D type C.perfringens strain are respectively inoculated on a blood plate culture medium for resuscitation and then are subjected to bacteria propagation anaerobic culture with nutrient broth, a C type C.perfringens bacterial liquid and a D type C.perfringens bacterial liquid are obtained respectively and then are cultured and inactivated by a toxigenic culture medium, and a serum is subjected to basic immunization and strengthening immunization and then is separated to obtain the product. The prepared C and D type C.perfringens antitoxin serum has the advantages of high titer, strong specificity and no toxic or side effect, can be used for immunization of animal C and D type C.perfringens diseases, and can play a great role in researches on pre-control of epidemic of sheep struck, enterotoxaemia and other diseases caused by infection of C type, D type or C and D two-type C.perfringens.

The invention provides a method for detecting the number of viable bacilli, which is characterized by comprising the following steps: weighing a bacillus sample according to a conventional method; adding the bacillus sample to a triangular flask or a test tube holding a nutrient broth germinant, wherein the broth germinant contains manganous sulfate, magnesium sulfate and ferrous sulfate; puttingthe triangular flask or the test tube in a constant-temperature water bath the temperature of which is 80 DEG C to carry out high-temperature treatment for 10-15 min; cooling with constant-temperaturerunning water; vibrating on a swing bed for 30 min, and then obtaining a bacillus culture solution; diluting the bacillus culture solution for 10 times with 0.5-1wt% of sodium chloride diluent; inoculating in a nutrient agar culture medium which is melted in advance and cooled to 50 DEG C, and after solidification, putting in a constant temperature cabinet the temperature of which is 37 DEG C tocarry out inverted culture for 48 hours, and then seeing that visible colonies grow out on a flat plate; and calculating according to the conventional method to obtain the number of the viable bacilliin the sample. The invention has the advantage of high accuracy.

The invention discloses a Burkholderia sp. Strain ES-89 for preventing and treating fungal diseases of plants as well as a culture method and application. The culture method comprises the following steps: A, inoculating an ES-89 strain into a nutrient broth culture liquid for activation; B, inoculating the activated ES-89 into a triangular flask with the nutrient broth culture liquid, and performing shake cultivation; C, centrifuging fermentation broth obtained from shake cultivation under a certain condition, and filtering an upper layer of fermentation broth by a bacterial filter to obtain sterile supernate; D, washing the bacterium with sterile water to prepare a bacterial suspension, and storing the fermentation broth, the fermentation supernate and the bacterial suspension. The strain is preserved with the preservation number of CCTCC, CCTCC NO: M 2016665, and the strain ES-89 can be applied to preparation of medicines for treating or preventing plant pathogenic fungi. The strain has an inhibition function on coptis chinensis leaf spot diseases and the like, and can be applied to prevention and treatment on multiple plant diseases. The prevention and treatment effect of the strain on the coptis chinensis leaf spot diseases can be 88% or greater, the culture method is feasible, the operation is simple and convenient, the pollution in the production process is reduced, solvent poisoning is avoided, and the application prospect is relatively good.

The invention relates to a method for producing high-quality high-protein feed through mixed strain solid-state fermentation of rice dregs and manioc waste. The method comprises the following steps: (1) preparation of liquid seed: culturing bacillus subtilis with a nutrient broth liquid medium, culturing aspergillus niger and beer yeast with a potato glucose medium respectively, and mixing the cultured strains aspergillus niger, beer yeast and bacillus subtilis at a volume ratio to obtain the liquid seed; (2) proportionally mixing the rice dregs (dry basis) and manioc waste (dry basis), adding magnesium sulfate, dipotassium phosphate and water, mixing uniformly and inoculating the liquid seed, and culturing for 5-7 days at 28-32 DEG C; (3) drying the fermented material at 40-60 DEG C and grinding to obtain high-protein feed. The method provided by the invention increases the feed value of the rice dregs and manioc waste, and improves the palatability of manioc waste as feed so that the manioc waste is easy to absorb by animals; meanwhile, the method also can improve the effective microbial community in the intestinal tracts of the animals, and has the beneficial effect of improving the immunity and disease resistance of the animals.

The invention discloses a microorganism repairing agent for repairing rock cracks and a preparation method of the microorganism repairing agent. The microorganism repairing agent is prepared from thefollowing raw materials of: bacillus pasteurii and a cementing material, the cementing material is prepared from the following raw materials in parts by weight: 17-35 parts of urea, 32-64 parts of calcium chloride, 10-20 parts of nutrient broth, 1.5-2.5 parts of NaHCO3 and 9-12 parts of NH4Cl; the OD600 value of the bacillus pasteurii in the repairing agent is 0.6-1.2. The microbial remediation agent provided by the invention has the advantages of small size, low viscosity, high permeability, environmental friendliness and the like, and can quickly enter narrow rock cracks to fill and seal thecracks so as to solve the problem of rock crack leakage; the product of microbial mineralization is mainly calcite type calcium carbonate; the product has good stability and durability and is good incompatibility with a matrix material; the method that microorganisms are adopted to induce calcium carbonate precipitation is an eco-friendly technology and has the advantage of being green and environmentally friendly.

The invention discloses a microbial reinforcement liquid for slope reinforcement, a preparation method and a construction method thereof, and relates to a microbial reinforcement liquid. The adhesivebinder is a sodium ion solution, strains of the mineralized bacteria solution A are bacillus subtilis and bacillus licheniformis, the OD600 value is 1.0-1.5, the mineralized bacteria solution A comprises distilled water, 3-5g / L of nutrient broth, 35-45g / L of urea, 65-85g / L of calcium chloride and 10-20g / L of ammonium chloride, and strains of the mineralized bacteria solution B are sporosarcina pasteurii and rhodopseudomonas; the OD600 value ranges from 0.5 to 1.0; the mineralized bacteria nutrient solution B comprises distilled water, 4-6g / L of nutrient broth, 25-35g / L of urea, 45-65g / L of calcium chloride and 10-20g / L of ammonium chloride. The mineralization ability of bacillus subtilis and sporosarcina pasteurii is utilized to carry out ecological reinforcement on the expansive soil slope.

The invention relates to a preparation method of a compound microbial flocculant for cyanobacterial bloom. The preparation method comprises the following steps: respectively inoculating Serratia and Bacillus into a sterilized nutrient broth medium for culture; respectively taking out 1mL of bacterial liquids of Serratia and Bacillus after culturing for 24h, and inoculating into a sterilized fermentation medium for fermentation culture; treating a broth as a seed liquid after culturing for 72 h and carrying out complex culture for 72h under conditions of a culture temperature of 35 DEG C, a rotation speed of 160rpm, an initial pH of a medium of 8.0, and an inoculation amount of the seed liquid of 10 ml / L; and carrying out centrifugal separation on the broth, and taking out a supernatant liquor. So the compound microbial flocculant is obtained. The preparation method is simple, fast and low cost, the flocculant prepared in the invention has the advantages of small dosage in use, strong stability and easy storage, can effectively flocculate cyanobactreial water samples with a flocculation rate of 92%, and has an excellent application prospect.

The invention discloses a preparation method of a Cynoglossus semilaevis vibrio anguillarum vaccine, which is characterized in that Cynoglossus semilaevis pathogeny Listonella anguillarum BH1 serves as an immunogen, and Astragalus mongholicus lixivium serves as an adjuvant. The preparation method comprises the following steps: inoculating the Cynoglossus semilaevis pathogeny Listonella anguillarum BH1 bacterial strain on a common nutrient broth culture medium; after the bacterial strain is cultured, inactivating by formaldehyde to obtain inactivated bacteria; after dipping the Astragalus mongholicus with distilled water, decocting, and filtering; condensing until the final concentration is 1g of raw drug per ml, and obtaining the Astragalus mongholicus lixivium; and mixing the inactivatedbacteria with the Astragalus mongholicus lixivium to obtain the Cynoglossus semilaevis Vibrio anguillarum vaccine. When the vaccine prepared with the method disclosed by the invention is used for immunizing Cynoglossus semilaevis, the capability of the Cynoglossus semilaevis on resisting Vibrio anguillarum infection can be obviously improved, Vibrio anguillarum infection during the culture production of the Cynoglossus semilaevis can be effectively prevented, the death rate of the Cynoglossus semilaevis is reduced, the culture yield of the Cynoglossus semilaevis is improved, the Cynoglossus semilaevis culture production is guaranteed to healthily and sustainably develop, and the economic benefit for Cynoglossus semilaevis culture production is improved.

The invention provides a method for counting the effective viable bacterium content of Bdellovibrio bacteriovorus powder, which comprises the following steps: appropriately diluting a sample to be detected, so that Bdellovibrio bacteriovorus in the sample to be detected is sufficiently dispersed into individual cells; then, carrying out incubation culture in an aseptic nutrient broth diluted by 10 times to promote growth and propagation, thus forming a megascopic turbid liquid; further determining that a large amount of Bdellovibrio bacteriovorus is contained in the turbid liquid having the maximum dilution through plaque detection; and finally, determining the total amount of effective viable bacteria in the sample to be detected. Compared with a microscopic direct observation method anda dilution-by-tap-water double-layer agar plate method, the method provided by the invention is more accurate, and overcomes the defects that detection is inaccurate due to dilution in the dilution-by-tap-water double-layer agar plate method and killed bacteria and viable bacteria can not be distinguished in the microscopic direct observation method.

The invention discloses a method for detecting drug sensitivity rapidly. The method includes the steps of 1, mixing nutrient broth with antibacterials to prepare an antibacterial diluent with gradientchange in concentration; 2, preparing a standard bacterial suspension of strain to be detected with super pure water, and diluting the broth for standby use; 3, respectively adding the standard bacteria suspension diluted by the broth of the same volume in the antibacterial diluent prepared in the step 1, dropwise adding the obtained mixture to a sample plate of a mass spectrometer for sample application according to the concentration gradient of the antibacterial diluent, and incubating for 2.0-5.0 hours after sample application is completed; 4, after incubation, discarding the broth, drying, dropwise adding a mass spectrometry identification pretreatment reagent, performing microbiological identification with the mass spectrometer after crystallization and reading the result.The pretreatment operation of the method is simple, accurate drug sensitivity result can be provided more quickly and intuitively, and a certain reference can be provided for clinical guidance and rational use of drugs in a timely manner.

The invention relates to methods and instruments for the rapid detection and rapid mass spectrometric identification of microbial infective agents in blood or other body fluids. The invention recognizes that blood is not a good environment for the cultivation of microbes and provides a method which (a) largely destroys or dissolves the human particles in body fluids, such as erythrocytes and leukocytes in blood, without impairing the ability of the microbes to reproduce, (b) separates the microbial pathogens from the fluid, (c) cultivates them in a nutrient broth which contains none of the antimicrobial components of the body fluids, (d) separates them from the nutrient broth, and (e) identifies the microbes by a mass spectrum of the microbial proteins. The dissolution of the human particles also releases the microbes nesting in macrophages. The cultivation in an optically clear nutrient broth with optimum composition not only accelerates the propagation of the microbes compared to all other cultivation methods, but also makes it possible to continuously measure their quantitative growth starting from a low microbe density. This firstly allows the mass spectrometric identification to be carried out at the earliest possible time, secondly provides a positive detection of microbes far ahead of their identification, which can be lifesaving for the patient; and thirdly makes it possible to start the determination of resistances early.

An insoluble phosphate bioleaching method is characterized in that insoluble phosphate raw materials are crushed and sieved by a 2mm sieve, bacillus is qualitatively and quantitatively measured by a phosphate dissolving ring and a liquid shake flask culture medium, efficient strains with high phosphate dissolving capacity is screened, 2-8ml screened efficient strains are inoculated into 100ml of nutrient broth media and cultured for 3-5 days under the condition of 25-35 DEG C and 100-150r / min to serve as bacteria, 10% of sucrose is added into clear water and serves as a nutrient source, 1-7% of insoluble phosphate raw materials are added, 2-8% of the cultured bacteria are inoculated and cultured for 7-10 days under the condition of 25-35 DEG C and 120-160r / min, and solid residues and culture solution are separated until the content of water-soluble phosphorus in the culture solution is higher than or equal to 100mg / L. The selected bacteria have an efficient phosphate dissolving function and are high in phosphate bioleaching rate, the content of beneficial microorganisms in soil can be increased, and phosphorus use rate is increased.

The invention relates to methods and instruments for the rapid detection and rapid mass spectrometric identification of microbial infective agents in blood or other body fluids. The invention recognizes that blood is not a good environment for the cultivation of microbes and provides a method which (a) largely destroys or dissolves the human particles in body fluids, such as erythrocytes and leukocytes in blood, without impairing the ability of the microbes to reproduce, (b) separates the microbial pathogens from the fluid, (c) cultivates them in a nutrient broth which contains none of the antimicrobial components of the body fluids, (d) separates them from the nutrient broth, and (e) identifies the microbes by a mass spectrum of the microbial proteins. The dissolution of the human particles also releases the microbes nesting in macrophages. The cultivation in an optically clear nutrient broth with optimum composition not only accelerates the propagation of the microbes compared to all other cultivation methods, but also makes it possible to continuously measure their quantitative growth starting from a low microbe density. This firstly allows the mass spectrometric identification to be carried out at the earliest possible time, secondly provides a positive detection of microbes far ahead of their identification, which can be lifesaving for the patient; and thirdly makes it possible to start the determination of resistances early.

The invention discloses an antibiotic active substance. The antibiotic active substance is from Bacillus atrophaeus, and a preparation method of the antibiotic active substance comprises the following steps: inoculating Bacillus atrophaeus into a nutritional broth medium, carrying out shaking table culturing, centrifuging the obtained cultured broth, collecting the obtained supernatant, and filtering the supernatant by a filter in order to obtain a bacterium-removed fermentation supernatant containing the antibiotic active substance. The invention further discloses a culturing, separating and purifying method of the antibiotic active substance.

The invention relates to piglet pellet feed preparation method which comprises the following steps: (1) micro pulverization and even mixing are conducted to complete feed through a conventional feed processing method; (2) the mixed complete feed micro powder is poured into an inclined rotary disk granulating machine, and vaporous adhesive nutrient broth is evenly sprayed on the complete feed micro powder in the disk granulating machine, to prepare spherical granular feed; (3) the spherical granular feed is spread on a mesh-belt drying machine, to be heated and dried; (4) the granular feed is cooled through a counterflow cooling method for conventional feed processing; the particles of the granular feed prepared by the method are more floppy and crispy; the method improves the palatability of piglets, meanwhile, improves the granulation efficiency, reduces the granulation energy consumption and saves the artificial cost of granulation.

The invention discloses a method for extracting active polysaccharide of wheat bran. The method comprises seed liquid preparation, fermentation medium preparation, fermentation and active polysaccharide extraction and concretely comprises inoculating nutrient broth with revived schizophyllum commune, bacillus subtilis, lactic acid bacteria and saccharomyces cerevisiae according to a weight ratio,carrying out culture to obtain a seed liquid, mixing wheat bran, soybean meal, corn core powder, distilled water and a fermentation synergist to obtain a fermentation medium, inoculating a fermentation medium with the seed liquid, carrying out fermentation culture, and extracting the fermented wheat bran through distilled water and an ethanol solution to obtain an active polysaccharide solution. The fermentation synergist can greatly increase the yield of the active polysaccharide. The method can significantly reduce the probability that the wheat bran active polysaccharide is separated alongwith the protein denatured gel and increase the yield and content of the wheat bran active polysaccharide. The wheat bran active polysaccharide can be used as an antioxidant active ingredient to remove free radicals in the body.

The invention relates to a method for treating mercury in water with escherichia coli. According to the method, escherichia coli is used and cultured in an enrichment mode in a nutrient broth medium to a stable growth period on the anaerobic condition, then the water containing divalent mercury is inoculated with escherichia coli, nutrient broth serves as nutrition to maintain bacterium growth, and anaerobic culture is carried out for several days on the room temperature condition to stop the experiment. The divalent mercury ions (Hg2+) in the sewage can be converted into mercuric chloride (HgCl) precipitate by escherichia coli, and mercuric chloride (HgCl) precipitate is difficult to dissolve in water, can stably exist in a water body and finally deposits to the bottom of the water body, so that divalent mercury pollution in the water body is removed, harm to the human body is reduced, and secondary pollution is avoided. The method has the advantages of being low in cost, wide in application range and the like.

The invention relates to a culture method for COD (Chemical Oxygen Demand) degrading bacteria, which includes the following steps: (1) a nutrient agar culture medium is prepared; (2) COD degrading bacteria are activated in the nutrient broth culture medium; (3) a pipette is used for respectively extracting 200mL of diluted COD degrading bacteria solution. The culture method has the advantages that the COD degrading bacteria cultured by the culture method can effectively increase the COD degradation rate, and cannot pollute the environment.

The present invention discloses primer sequence and detecting reagent kit for detecting delayed Edward's bacteria. The detecting reagent kit contains the following reagents: mixture of nutrient broth and aged sea water through autoclaving; mixture of PCR buffer, Taq DNA polymerase and sterile double distilled water; mixture of nucleotide sequence of SEQ ID No. 1 and SEQ ID No. 2, dNTPs and MgCl2; genome total DNA of delayed Edward's bacteria; sterile double distilled water; TBE electrophoretic buffer containing Tris-boric acid and EDTA; and agarose containing ethidium bromide. The present invention has primer sequence with optimized PCR condition and system, and the PCR detecting method for detecting delayed Edward's bacteria and the detecting reagent kit are simple, fast, sensitive and specific.

The invention relates to an application of bacillus subtilis in antagonism helicobacter pylori, wherein substances capable of restraining the growth of helicobacter pylori can be generated when the shake cultivation is performed on the bacillus subtilis for 9 hours at 37 DEG C and at a speed of 180 rpm (revolutions per minute) in a nutrient broth liquid culture medium, thereby efficiently restraining the growth of the helicobacter pylori; when the bacillus subtilis is cultivated for 18 hours, the antibiotic ability is maximum; and antagonistic proteins are acquired by purifying the substances, thereby preparing a preparation for treating gastrointestinal tract diseases. The invention has the following technical effects: the bacillus subtilis from probiotics has a function of maintaining the micro ecological balance of an intestinal tract; the bacillus subtilis culture has stronger antagonism to the helicobacter pylori; and compared with the antibiotic treatment method used for conventionally treating the helicobacter pylori, the bacillus subtilis can be used for overcoming the defects that the side effect by using antibiotic treatment method is strong and the symptoms are easily reappeared after the disease is treated, and the like.

The invention discloses a microbial detection method for the antibacterial property of a disposable hygienic product. The microbial detection method comprises the following steps of: detecting bacterial microorganism and fungus microorganism. Simulative menses is prepared from a nutrient broth culture medium and a Sabouraud liquid culture medium respectively, nutritional substances contained in the simulative menses can be used for simulating human body blood to supply a good growing environment for bacteria, and an environment which is close to a human body is provided for a sample wafer, so that the application environment of the disposable hygienic product is better simulated, and the experimental accuracy is ensured.

The invention discloses sewage enterovirus reclaiming method. It includes the following steps: adding poly-aluminum chloride or aluminum chloride in sewage; adjusting pH 5.5-8.5 and temperature 4-30 centigrade degree; passing through filtering column; eluting by six times nutrient broth and collecting the eluent; adding polyethylene glycol with 6000 molecular weight, poly-aluminum chloride or aluminum chloride into the eluent; centrifugation and removing the supernatant; adding normal saline to do suspensoid settlement and constant volume. The invention can make the virus recovery rate rise to over 80%. The invention has the advantages of cheap related tool material sources, high virus recovery rate, large sewage disposal quantity, and stable effect.