The invention relates to a method for remedying acidified
arsenic contaminated soil by
biochar-loaded nano-scale zero-valent iron cooperated with
bacteria. The method includes selecting
Pseudomonas putida strain MnB1 (ATCC23483); carrying out
enrichment culture on the
Pseudomonas putida strain in
enrichment culture media; inoculating strains in culture media with
divalent manganese and carrying out culture on the strains to obtain active metabolites; adding the active metabolites and the green synthetic
biochar-loaded nano-scale zero-valent iron into the acidified
arsenic contaminated soil anduniformly stirring the active metabolites, the
biochar-loaded nano-scale zero-valent iron and the acidified
arsenic contaminated soil; carrying out a series of physical-
chemical reaction on active
manganese oxide, zero-valent iron, biochar and trivalent arsenic or pentavalent arsenic in the soil; converting the arsenic in exchangeable forms into arsenic in residual forms. The method has the advantages that the arsenic in the soil can be effectively immobilized, the pH (potential of
hydrogen) of the soil can be increased, and the double purposes of remedying
soil acidification and arsenic
contamination can be simultaneously achieved; the method is short in remediation time, high in efficiency, wide in treatment range and free of secondary
pollution, and stable effects can be realized.