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Method for remedying acidified arsenic contaminated soil by biochar-loaded nano-scale zero-valent iron cooperated with bacteria

A nano-zero-valent iron and biochar technology is applied in the restoration of polluted soil, which can solve the problems of large dosage, long repair time and low repair efficiency, and achieve the effect of reducing dosage

Active Publication Date: 2019-04-05
QINGDAO TECHNOLOGICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional physical-chemical remediation technology usually has a large dosage, high cost, and is prone to secondary pollution, so it is not suitable for the remediation of large areas of arsenic-contaminated soil
Phytoremediation and microbial remediation have the advantages of low cost, no damage to soil and river ecological environment, and no secondary pollution, but there are also disadvantages such as low remediation efficiency and long remediation time

Method used

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  • Method for remedying acidified arsenic contaminated soil by biochar-loaded nano-scale zero-valent iron cooperated with bacteria
  • Method for remedying acidified arsenic contaminated soil by biochar-loaded nano-scale zero-valent iron cooperated with bacteria
  • Method for remedying acidified arsenic contaminated soil by biochar-loaded nano-scale zero-valent iron cooperated with bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1) Enrichment culture of bacterial strains

[0041] Pseudomonas putida P. putida Strain MnB1 was inoculated in Pseudomonas putida enrichment medium A at a volume ratio of 5%, and then aerobic enrichment culture was carried out at 25°C under horizontal shaking (150 rpm) for 2 days; the components of medium A Yeast extract 0.3g, hydrolyzed casein 0.2g, glucose 0.3g, calcium chloride 0.1g, magnesium sulfate 0.1g, trace elements 1mL, deionized water 1 liter;

[0042] The selected bacterial strains come from the American Type Bacteria Collection Center with the preservation number ATCC 23483.

[0043] 2) Preparation of active metabolites

[0044]Add the Pseudomonas putida bacterial solution enriched in step 1) to Pseudomonas putida culture medium B with a pH of 7.2 at the same time as 1.2g / L manganese carbonate at an inoculation amount of 5% by volume, and then at 25°C Cultivate under aerobic conditions for 7 days, and brown-black flocs appear in the medium, which means t...

Embodiment 2

[0054] 1) Enrichment culture of bacterial strains

[0055] Pseudomonas putida P. putida Strain MnB1 bacteria were inoculated in Pseudomonas putida enrichment medium A according to the transfer amount of 10% by volume, and the components of the medium A were yeast extract 0.8g, hydrolyzed casein 0.8g, glucose 0.8g, Calcium 0.4 g, magnesium sulfate 0.6 g, trace elements 5 mL, deionized water 1 liter, and then at 35 °C under horizontal shaking (180 rpm), aerobic enrichment culture for 5 days;

[0056] The selected bacterial strains come from the American Type Bacteria Collection Center with the preservation number ATCC 23483.

[0057] 2) Preparation of active metabolites

[0058] Add the Pseudomonas putida bacterial solution enriched in step 1) to the Pseudomonas putida culture medium B with a pH of 7.5 at the volume ratio of 10%, and add 0.8 g / L manganese carbonate at the same time. The composition of medium B is 0.45 g of ferrous ammonium sulfate, 0.5 g of sodium citrate, 0....

Embodiment 3

[0067] 1) Enrichment culture of bacterial strains

[0068] Pseudomonas putida P. putida Strain MnB1 was inoculated in Pseudomonas putida enrichment medium A at a volume ratio of 8%, and then aerobic enrichment culture was carried out at 25°C for 4 days under horizontal shaking (150 rpm);

[0069] The components of the medium A are 0.5 g of yeast extract, 0.4 g of hydrolyzed casein, 0.5 g of glucose, 0.5 g of calcium chloride, 0.4 g of magnesium sulfate, 3 mL of trace elements, and 1 liter of deionized water;

[0070] The selected bacterial strains come from the American Type Bacteria Collection Center, with the preservation number ATCC 23483;

[0071] 2) Preparation of active metabolites

[0072] Add the Pseudomonas putida bacterial solution enriched in step 1) to the Pseudomonas putida medium B with a pH of 6.8 at an inoculum volume of 6% by volume, and add 1.0g / L manganese carbonate at the same time, and then Cultivate under aerobic conditions at 30 degrees Celsius for 7 ...

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Abstract

The invention relates to a method for remedying acidified arsenic contaminated soil by biochar-loaded nano-scale zero-valent iron cooperated with bacteria. The method includes selecting Pseudomonas putida strain MnB1 (ATCC23483); carrying out enrichment culture on the Pseudomonas putida strain in enrichment culture media; inoculating strains in culture media with divalent manganese and carrying out culture on the strains to obtain active metabolites; adding the active metabolites and the green synthetic biochar-loaded nano-scale zero-valent iron into the acidified arsenic contaminated soil anduniformly stirring the active metabolites, the biochar-loaded nano-scale zero-valent iron and the acidified arsenic contaminated soil; carrying out a series of physical-chemical reaction on active manganese oxide, zero-valent iron, biochar and trivalent arsenic or pentavalent arsenic in the soil; converting the arsenic in exchangeable forms into arsenic in residual forms. The method has the advantages that the arsenic in the soil can be effectively immobilized, the pH (potential of hydrogen) of the soil can be increased, and the double purposes of remedying soil acidification and arsenic contamination can be simultaneously achieved; the method is short in remediation time, high in efficiency, wide in treatment range and free of secondary pollution, and stable effects can be realized.

Description

technical field [0001] The invention belongs to the technical field of soil remediation treatment; in particular, it relates to a method for biochar-loaded nanometer zero-valent iron to cooperate with bacteria to remediate acidified arsenic-contaminated soil. technical background [0002] Arsenic (As) is a highly toxic metalloid, which can cause various cancers such as skin cancer and bladder cancer, and poses a serious threat to human health. The sources of arsenic in soil are mainly divided into natural sources and anthropogenic sources. Natural sources are mainly natural activities, such as volcanic eruptions, crustal movement, and the release of some arsenic-containing minerals. The anthropogenic sources of arsenic mainly include the processes of mining, industrial production and agricultural production. First of all, arsenic often coexists with some metals during mineral mining and smelting, so arsenic will enter water, air and soil in various forms during the mining ...

Claims

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Application Information

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IPC IPC(8): B09C1/10B09C1/08
CPCB09C1/08B09C1/10
Inventor 王华伟吴雅静王亚楠孙英杰高莹汉红燕
Owner QINGDAO TECHNOLOGICAL UNIVERSITY
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