137 results about "Phytoremediation" patented technology

The invention discloses a factory soil and groundwater pollution risk management and control method. The method comprises the following steps: acquiring a factory data set at a current moment and a historical moment; establishing a risk prediction model based on the factory data set; dividing risk grade distribution in the factory based on the risk prediction model and repairing the risk grade distribution; for the high-risk area, in-situ chemical oxidation and a permeable reactive barrier are adopted for repairing; phytoremediation or microbial remediation is adopted for medium risk areas; a low-risk area does not need to be repaired; the method can accurately predict the risk grade distribution in the factory according to the geological parameters, pollutant monitoring data and environmental dynamics of the factory, realizes risk grading output by constructing a risk prediction model fusing a physical mechanism and machine learning advantages, improves the accuracy and repair efficiency of risk assessment, and improves the risk assessment efficiency. And the pertinence, the economical efficiency and the environmental benefits of pollution control are remarkably improved.

The invention discloses a method for improving cadmium accumulation and transport capacity of sedum plumbizincicola, and relates to the field of plant heavy metal pollution remediation. The method comprises the following steps: preparing a selenium solution with the selenium ion concentration of 5 [mu] M, adding the selenium solution into the cadmium-polluted water environment, planting sedum plumbizincicola seedlings in the cadmium-polluted water environment, and carrying out cultivation management; plant pretreatment: culturing sedum plumbizincicola seedlings in non-polluted soil for at least three generations, selecting sedum plumbizincicola plants which are uniform in size and healthy in growth vigor, and pre-culturing the sedum plumbizincicola plants in a Hoaglands nutrient solution for two weeks for rooting; the healthy plants are transferred into Hoagland nutrient solution containing selenium solution to be cultured, and the plants are harvested after 28 days. According to the method, the high-concentration cadmium polluted water environment is simulated, and selenium is applied in the growth period of the sedum plumbizincicola, so that absorption and enrichment of the sedum plumbizincicola on cadmium are promoted, the plant biomass is improved, the toxic effect of cadmium on plants is relieved, and efficient remediation of the sedum plumbizincicola on the cadmium polluted environment is achieved.

The invention relates to the technical field of phytoremediation of Cd-polluted soil. The invention provides Cd-resistant bacillus cereus as well as application and a method of the Cd-resistant bacillus cereus in remediation of Cd-polluted soil, the preservation name of the bacillus cereus is Bacillus cereus 01, the Latin name of the bacillus cereus is Bacillus cereus, the bacillus cereus is preserved in Guangdong Microbial Culture Collection Center, the preservation number of the bacillus cereus is GDMCC No: 65570, and the preservation time is December 2, 2024. The efficient Cd-resistant endophytic bacteria are obtained through screening and identification, the Cd-resistant endophytic bacteria are combined with the smooth vetch to repair Cd-polluted soil, absorption of succeeding crops to Cd is reduced, the food safety problem caused by standard exceeding of Cd is reduced, and the method has the advantages of being low in cost, high in repair efficiency, simple and convenient in treatment step and the like.

The invention discloses a method for promoting phytoremediation of heavy metal contaminated soil by combining modified charcoal with microorganisms, and relates to the technical field of heavy metal contaminated soil remediation. The repairing agent is prepared and comprises an inner layer, a middle layer and an outer layer, envelopes are arranged among the layers, the outer layer is further coated with an outer envelope, the inner layer comprises modified biochar and a first microbial agent, the middle layer comprises modified biochar and a second microbial agent, and the outer layer comprises dolomite powder and oyster shell powder. According to the invention, the remediation agent is combined with the heavy metal enrichment plants to carry out synergistic remediation on the contaminated soil, so that the enrichment plants can grow well under the high-acid high-concentration heavy metal environment condition, the problem of low biomass of the enrichment plants is solved, and finally, the efficient remediation of the contaminated soil is realized.

The invention relates to the technical field of tailing sand treatment, in particular to a method for repairing vanadium titano-magnetite tailing sand by utilizing plants, which comprises the following steps: ploughing and flattening the surface layer of the tailing sand, then applying an organic modifier and a pH regulator, and fully mixing with the tailing sand of 20-40cm on the surface layer; synchronously applying a microbial agent containing heteroform rhizopus in the plant growth substrate, and sowing bermuda grass; performing field management after the cynodon dactylon seedlings survive; after at least one complete growth cycle of the cynodon dactylon is completed and the cynodon dactylon enters a mature period, stubbles of 5-10 cm are reserved by using a harvesting machine for cradling so as to ensure that the cynodon dactylon keeps necessary growing points, so that the cynodon dactylon can be quickly regenerated in the next growing season, and then the cradled cynodon dactylon is treated. The phytoremediation efficiency of the vanadium titano-magnetite tailing sand can be improved, and ecological restoration, resource circulation and environmental safety are also considered.

The invention discloses a method for repairing a karst mountain land in an extremely arid area under the condition of no soil collapse, belongs to the field of mine repairing, and aims to solve the problems that a certain strip mine belongs to a desert gobi area, the main type of soil is gypsum ash brown desert soil, the precipitation amount is small, the evaporation amount is large, and phytoremediation is difficult. The remediation object is the desert gobi area mine soil, the soil is mud left after mineral products are removed and the mineral products are deeply excavated by hundreds of meters, and the mud comes from hundreds of meters underground and hardly contains microorganisms; meanwhile, the saline-alkali content of the soil is very high. Different from the prior art, the remediation method provided by the invention adopts a manner of not killing soil, adopts the suaeda salsa as a remediation plant, and adopts a drip irrigation manner to ensure the water supply of the suaeda salsa, so that the remediation of the mine soil is realized. According to statistics, the total irrigation quota of the whole growth period of the suaeda salsa is 80-100 m < 3 > / mu, and the water consumption is obviously lower than the water consumption required by an existing remediation scheme.

The invention discloses a method for repairing heavy metal contaminated soil through plants and recycling the heavy metal contaminated soil. According to the method, a proper amount of iron-containing fertilizer is applied while phytoremediation is conducted, iron nutrients are provided for plants, and heavy metal contaminated soil is remedied in cooperation with the plants. Restoration plants are rich in biomass after being harvested, biological source potassium humate can be prepared, reduction, harmlessness and recycling are achieved, iron in the plants plays a catalytic role in the process, and the yield of the potassium humate can be increased. As an efficient organic potash fertilizer, potassium humate can improve soil quality, promote plant growth and improve crop stress resistance, potassium humate prepared from remediation plants is recycled to polluted soil, soil remediation is promoted, virtuous cycle of soil and plants is achieved, the rest of potassium humate can be sold, and economic benefits are obtained.

The invention discloses an ecological treatment and resource utilization method for lake polluted bottom mud, and belongs to the technical field of environmental governance and resource recycling. The method comprises the following steps: S1, bottom mud collection and pretreatment; s2, microbial enhanced remediation; s3, phytoremediation synergistic effect; and S4, carrying out post-treatment and recycling on the bottom mud. According to the method, an ecological treatment means is adopted in the whole process, the synergistic effect of microorganisms and plants is utilized, large-scale use of chemical agents is avoided, and the risk of secondary pollution is reduced; through multi-stage treatment of microbial enhanced remediation and phytoremediation, the obvious removal effect on organic pollutants, heavy metals and nutrient substances is achieved; original waste polluted bottom mud is converted into practical products such as building materials and soil amendments, so that the treatment cost of the bottom mud is reduced, and economic added values are created; the process parameters can be flexibly adjusted according to different lake pollution types, degrees and peripheral requirements, and the method is suitable for bottom mud treatment and resource recovery of lakes with various scales and various functions.

The invention discloses a tree species screening method for heavy metal lead contaminated soil remediation, and relates to the technical field of heavy metal lead contaminated ecological management. Common greening tree species such as cinnamomum camphora, willow, gingko, koelreuteria paniculata, photinia serrulata and osmanthus fragrans are selected, leaf and rhizosphere soil are collected in a point distribution mode, and the content of heavy metal Pb in a sample is measured; the heavy metal Pb content distribution characteristics of different tree species are quantified, the heavy metal Pb enrichment capacity difference of the greening tree species is explored, the heavy metal Pb enrichment and transfer efficiency of the greening tree species is comprehensively evaluated, and the greening tree species with the best heavy metal Pb purification effect is screened out. The method is a phytoremediation technology which has the advantages of being economical, simple, convenient, high in recognition degree, long in life cycle, free of damage to the soil structure, not prone to causing secondary pollution and the like.

The present invention discloses Sesbania cannabina Nramp5 genes and their applications in hyperaccumulating cadmium, belonging to the technical field of genetic engineering. The present invention clones the Sesbania cannabina ScNramp5 gene for the first time. By expressing the ScNramp5 gene in yeast, it is preliminarily determined that Sesbania cannabina ScNramp5 has the ability to transport, absorb and accumulate cadmium. Further, after overexpressing the ScNramp5 gene in rice, it is found that the cadmium content in the roots of the overexpressed ScNramp5 rice is significantly higher than that of the wild type, while the change in the cadmium content in the above-ground part is not obvious. This result indicates that ScNramp5 can efficiently accumulate cadmium and only efficiently accumulates cadmium in the roots. It provides an important theoretical basis and gene resources for using phytoremediation of cadmium-polluted soil and improving the cadmium tolerance of crops.

The invention discloses a combined phytoremediation method for strictly controlling cadmium pollution of farmland, and belongs to the technical field of farmland heavy metal pollution treatment and chelating induced plant extraction remediation. Aiming at the problems of low cadmium pollution remediation efficiency, high cost, poor economic benefit, high soil secondary pollution risk and the like of strictly controlled cultivated land, a biodegradable and environment-friendly efficient chelating agent is screened, economic crops with agricultural regulation and large biomass and the like are combined to carry out enhanced plant extraction remediation, a traditional single static remediation measure is broken through, and the remediation effect is good. Dynamic regulation and control are conducted according to nutrient requirements of plants in different growth periods, remediation and fertilization are both considered, planting structure adjustment is achieved, production, remediation and income can be achieved at the same time, and secondary pollution to soil cannot be caused. The method has higher repairing efficiency, lower repairing cost and better economic benefits, and has a good market application prospect.

The invention discloses a lake water ecological restoration monitoring method based on big data, and relates to the technical field of submerged plant monitoring, and the method comprises the following steps: collecting original water quality data of a to-be-restored lake area before the to-be-restored lake area is restored by using submerged plants, and collecting initial water quality data of the to-be-restored lake area after the to-be-restored lake area is restored by using the submerged plants; carrying out correlation analysis treatment to obtain related water quality physicochemical indexes; collecting water quality physicochemical data of related water quality physicochemical indexes of the lake area to be restored, and performing water quality physicochemical change analysis to obtain plant related change data; monitoring and analyzing the submerged plants in the lake area to be restored based on the related change data of the plants to obtain related change information of the submerged plants; the method is used for solving the problem that the development condition of submerged plants cannot be accurately obtained through water body parameters on the premise of not damaging the submerged plants when the submerged plants are monitored at the initial stage of repairing lake water ecology by using the submerged plants in the existing submerged plant monitoring technology.

The invention provides a data processing method and system for tailing pond phytoremediation, and relates to the field of data processing, and the method comprises the steps: obtaining a plurality of historical tailing pond phytoremediation cases; based on a plurality of historical tailing pond phytoremediation cases, a multi-dimensional remediation factor set is determined, and the multi-dimensional remediation factor set comprises a target tailing component factor, a target soil state factor and a target meteorological factor; based on the multi-dimensional repair factor set, collecting repair auxiliary features of the to-be-repaired tailings pond; searching a reference historical tailing pond phytoremediation case from a plurality of historical tailing pond phytoremediation cases based on the remediation auxiliary features of the to-be-remedied tailing pond; based on the reference historical tailing pond phytoremediation case, determining a phytoremediation strategy of the to-be-remedied tailing pond; in the process of executing the phytoremediation strategy of the to-be-remedied tailings pond, the real-time remediation data of the to-be-remedied tailings pond is collected, remediation effect evaluation is conducted, and the method has the advantage that the efficiency and reliability of phytoremediation of the tailings pond are improved.

The invention provides application of an arabidopsis thaliana CPK12 gene in improvement of lead stress resistance of plants. The nucleotide sequence of the arabidopsis thaliana CPK12 gene is as shown in SEQ ID NO.1, and the CDS sequence of the arabidopsis thaliana CPK12 gene is as shown in SEQ ID NO.3. The invention also provides application of the arabidopsis thaliana CPK12 gene in improvement of lead stress resistance of the plants. The arabidopsis thaliana CPK12 gene is used for negative regulation of the lead stress tolerance of plants. According to the application, an arabidopsis thaliana CPK12 gene is adopted as a negative regulatory factor to participate in a new function of responding to lead stress of a plant, an RNA interference vector RNAi targeting the arabidopsis thaliana CPK12 gene is constructed and is transformed into wild-type arabidopsis thaliana by utilizing a genetic engineering technology, and a homozygous transgenic line with a silent CPK12 gene expression is obtained through resistance screening. Experiments prove that the resistance of a transgenic line to lead stress is remarkably enhanced, which indicates that the CPK12 gene plays a negative regulation role in a plant lead stress response pathway. The invention provides a new molecular target for regulating and controlling the heavy metal stress resistance of plants by using the CPK12 gene, and has important application value in the field of phytoremediation of heavy metal contaminated soil and in the aspect of molecular breeding of lead-resistant crop varieties.

The invention relates to the technical field of phytoremediation of soil polluted by organophosphate flame retardants / plasticizers, in particular to a method for remedying soil polluted by chlorinated organophosphate by using compositae plant jerusalem artichoke. The compositae plant jerusalem artichoke is planted in the soil polluted by the chlorinated organophosphate, and the plant degrades, removes or remarkably reduces the content of phosphate tri (2-chloroethyl) ester, phosphate tri (1-chloro-2-propyl) ester and phosphate tri (1, 1-dichloro-2-propyl) ester in the soil through the synergistic effect of absorption and root system secreted substances in the growth process. And the content of chlorinated organophosphorus esters such as 2, 3-dichloro-2-propyl) ester and the like is increased. According to the method, the energy plant jerusalem artichoke capable of being used for producing bioethanol or methane is adopted for repairing the soil polluted by the chlorinated organophosphate, the method has the advantages of being small in investment, not high in technical requirement, capable of being used for in-situ repairing and the like, and the situation that the chlorinated organophosphate in the soil enters a food chain through plants and then harms human health can be avoided; meanwhile, raw materials can be provided for production of biological energy.

The invention belongs to the technical field of river and lake sediment remediation, and discloses a foldable device for in-situ electric-phytoremediation of river and lake heavy metal polluted sediment. Comprising a horizontally arranged insulating tube; the longitudinal frame comprises two supporting pipes, a first connecting piece and a second connecting piece, the two supporting pipes are vertically arranged and connected to the bottoms of the two insulating pipes respectively, a first through hole and a second through hole which are vertically formed are formed in the first connecting piece, a third through hole is formed in the second connecting piece, and two inclined supporting rods are connected to each supporting pipe; the two inclined supporting rods are detachably connected to the two ends of the same insulating tube respectively, and the longitudinal frame is made of insulating materials. The horizontal rotating shaft is arranged in the first through hole of the first connecting piece in a penetrating manner; the aquatic plant planting plate is arranged on the horizontal frame; the cathode electrode stem and the anode electrode stem are horizontally mounted in a second through hole and a third through hole which are provided with a plurality of through holes in the wall surface respectively; and the anode and the cathode of the power supply are respectively connected through wires.

The application discloses a kind of factory area soil and groundwater pollution risk control method, comprising: obtaining current time and historical time under factory area dataset;Based on the factory area dataset, establish risk prediction model;Based on risk prediction model, the risk level distribution in factory area is divided and repaired;For high-risk area, in-situ chemical oxidation and permeation reaction wall are used for repair;For medium-risk area, plant repair or microbial repair is used;Low-risk area does not need to be repaired;The method of the present application can accurately predict the risk level distribution in the factory area according to the geological parameters of the factory area, the pollution monitoring data and the environmental dynamics, by constructing a risk prediction model that integrates the physical mechanism and the advantages of machine learning, the risk classification output is realized, the accuracy of risk assessment and the repair efficiency are improved, and the pertinence, economy and environmental benefits of pollution control are significantly improved.

This invention discloses a method for improving saline-alkali land in semi-arid regions to enhance plant diversity, comprising the following steps: S1, construction of treatment units; S2, arrangement of treatment units; S3, preliminary soil treatment; S4, planting of plants; and S5, intensive soil treatment. This invention addresses the problems of high salinity and species scarcity in saline-alkali land in semi-arid regions, providing a complete treatment method. By deploying multiple treatment units to form a treatment system, combined with the soil conditioner specifically designed for this invention, the high salinity of saline-alkali soil in semi-arid regions can be alleviated. Simultaneously, by incorporating a phytoremediation system primarily composed of alkali plantations, the biodiversity of exposed saline-alkali land in semi-arid regions can be effectively enhanced. Furthermore, considering seasonal factors and other factors such as rainfall, a one-year treatment plan is ultimately developed. After treatment, the land can be selectively developed into ornamental wetlands or agricultural land, possessing significant economic value.

The present invention discloses a water-holding device applicable to phytoremediation of peak cluster ridges in karst areas, comprising a fixing device and a water-holding layer. The fixing devices are arranged at both ends of the water-holding layer, and connection holes are provided on the fixing devices. By fixing it to the area to be repaired with hemp ropes or springs, water is supplemented and plants are transplanted by division, thereby providing water for plant growth. Using layered water-holding matrix materials with different water-holding effects, the water-holding effect is better. The present invention can be flexibly configured and used under the complex terrain conditions of peak cluster ridges, achieving an efficient water-holding effect, improving the efficiency of vegetation restoration in karst areas, reducing the repair cost, and improving the current situation of soil and water loss in peak cluster ridges of karst areas.

The invention provides a method for improving phytoremediation of heavy metal cadmium / lead contaminated soil by using beta-cyclodextrin, and belongs to the technical field of heavy metal contaminated soil remediation. The method comprises the following steps: applying beta-cyclodextrin into the heavy metal cadmium / lead polluted soil, carrying out soil flooding treatment, and planting cadmium / lead tolerant and enriched plants; the application amount of the beta-cyclodextrin is 5-25 g / m < 2 >. According to the method, the enrichment capacity of plants to cadmium / lead is remarkably improved through beta-cyclodextrin, and test data shows that after beta-cyclodextrin is applied, the cadmium content of the roots, stems and leaves of amaranthus hypochondriacus is remarkably improved compared with that of a blank group, the repair period is greatly shortened, and the defect that traditional plant repair efficiency is low is overcome. The method has the advantages of being green, safe, easy to operate, quick in effect taking and high in practicability.

The invention discloses a slow-release activating agent for strengthening phytoremediation of heavy metal contaminated soil and a preparation method of the slow-release activating agent, and belongs to the technical field of heavy metal contaminated soil remediation. The method comprises the following steps: by taking sodium alginate as a carrier, embedding a biodegradable chelating agent glutamic acid tetrasodium diacetate (GLDA), and carrying out Fe < 3 + > gradient cross-linking and ethanol phase separation treatment, so as to construct the microsphere slow-release agent with a hierarchical porous structure with a compact outer layer and a loose inner layer. The microsphere slow-release agent prepared by the invention can realize intelligent slow release of GLDA, avoids initial burst release, reduces the risk of heavy metal leaching, improves the absorption efficiency of plants to heavy metals, has a remarkable repair effect, and has good environmental adaptability and popularization and application prospects.

The invention belongs to the technical field of environmental restoration, and particularly relates to cyclodextrin cross-linked supramolecular hydrogel as well as a preparation method and application thereof. A construction unit of the supramolecular hydrogel comprises double-bond modified beta-cyclodextrin and acrylic acid interacting with the double-bond modified beta-cyclodextrin through a covalent bond; plant hormones are included in a cavity of the double-bond modified beta-cyclodextrin. The supramolecular hydrogel adhesive tape is provided with charges and cavities, can include plant hormones, and can be used together with soil improvement plants such as green manure to enhance the saline-alkali soil improvement effect by regulating and controlling the salinity of plant rhizosphere soil, improving the emergence rate, promoting plant growth and improving the environmental stress resistance of the plants. Cavities of the double-bond modified beta-cyclodextrin can effectively include plant hormones, and the supramolecular hydrogel is formed through covalent interaction between the acrylic acid and the double-bond modified beta-cyclodextrin. Based on multi-covalent and non-covalent interaction, the supramolecular hydrogel has the advantage of controllably releasing plant hormones, and realizes sustainable environmental remediation by cooperating with plant remediation.

The invention discloses a preparation method and application of a bionic targeted three-dimensional porous cellulose microsphere heavy metal stabilizing agent, and the preparation method comprises the following steps: firstly, reacting ferric chloride hexahydrate with tannic acid to prepare TA-Fe; then, sodium carboxymethyl cellulose and TA-Fe are used as raw materials, and under the crosslinking action of aluminum nitrate, three-dimensional porous cellulose microspheres TA-Fe coated CMC with a metal-phenolic aldehyde network structure are prepared; and adding 4-(4, 6-dimethoxytriazine-2-yl)-4-methylmorpholine hydrochloride and aspartic acid, carrying out a stirring reaction, washing with deionized water, and carrying out freeze drying to obtain the final product. The preparation method is simple and convenient, the reaction condition is mild, and the raw material cellulose is green, environment-friendly and wide in source. The obtained stabilizing agent can efficiently, rapidly and selectively adsorb Pb, effectively reduces the bioavailability of Pb in soil, weakens the toxicity of Pb to plants, and can achieve a synergistic effect with a phytoremediation technology.

The pantoea agglomerans SE16 is classified and named as pantoea agglomerans, and is preserved in the China General Microbiological Culture Collection Center on October 17, 2024, the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 32246. The bacterial strain is applied to strengthening phytoremediation of heavy metal pollution, improving soil nutrients, promoting plant growth and strengthening phytoremediation of pollutants. The strain has the functions of dissolving phosphorus, fixing nitrogen, producing iron carriers, producing indoleacetic acid, ACC deaminase and cellulase, resisting acid, resisting salt and the like, and also can tolerate various heavy metals, polycyclic aromatic hydrocarbons and combined pollution of the heavy metals and the polycyclic aromatic hydrocarbons; by promoting the growth of the sudangrass, improving the photosynthesis of the sudangrass, relieving the oxidation pressure of the sudangrass and changing the composition of soil microbial communities, the toxicity of chromium to the sudangrass is relieved, and the method has practical popularization and application values.

This invention discloses a method for enhancing the cadmium accumulation and translocation capacity of *Sedum aizoon*, relating to the field of phytoremediation of heavy metal pollution. The invention includes the following steps: adding a 5 μM selenium ion concentration solution to a cadmium-polluted water environment; planting *Sedum aizoon* seedlings in the cadmium-polluted water environment for cultivation management; plant pretreatment: after culturing *Sedum aizoon* seedlings in unpolluted soil for at least three generations, selecting uniformly sized and healthy *Sedum aizoon* plants for pre-culturing in Hoaglands nutrient solution for two weeks to induce rooting; hydroponic treatment: transferring healthy plants to Hoaglands nutrient solution containing selenium for cultivation, and harvesting the plants after 28 days. This invention, by simulating a high-concentration cadmium-polluted water environment and applying selenium during the growth period of *Sedum aizoon*, promotes the absorption and accumulation of cadmium by *Sedum aizoon*, increases plant biomass, alleviates the toxic effects of cadmium on plants, and achieves efficient remediation of cadmium-polluted environments by *Sedum aizoon*.

The application provides application of a wild pea in repairing radium-polluted soil, and the wild pea is planted in radium-polluted soil for cultivation and management, when the wild pea grows to a mature stage, the aboveground part of the wild pea is removed from the polluted soil, or the wild pea is completely removed from the polluted soil; the above process is repeated until the radium content in the soil reaches an environmental safety standard. The wild pea is used to repair acidic radium-polluted soil in a subtropical monsoon climate zone, the wild pea grows normally in the acidic soil with low-level radioactive pollution and has no toxic and harmful phenomena. The wild pea at the mature stage has a high transfer coefficient and enrichment coefficient, the total radium removal amount of the aboveground part of the wild pea accounts for more than 80% of the total radium removal amount, and radium can be extracted from the low-level radium-polluted soil. The plant repair method provided by the application has simple operation process and convenient management, does not damage the basic structure and properties of the soil, and is suitable for repairing large-area acidic radium-polluted soil with low level.

The invention discloses intelligent optimization of soil microbial community and plant synergistic remediation in a phytoremediation experimental facility, and a system monitors soil pollutants, microbial metabolites and plant root exudates in real time, utilizes a microbial sensor and a nano-robot modified by synthetic biology, and an optogenetics technology to realize intelligent optimization of soil microbial community and plant synergistic remediation. Plant-microorganism interaction, a quantum annealing algorithm and a federated learning framework are optimized to generate an optimal repair strategy, the bionic mechanical arm and the laser-nanotechnology execute precise operation, the microbial fuel cell and the piezoelectric energy recovery unit realize self-energy supply of the system, and the system has the characteristics of high efficiency, intelligence and environmental friendliness and is suitable for large-scale popularization and application. The method is suitable for repairing complex soil pollution.

The invention relates to the technical field of phytoremediation, and provides a method for exploring chelating of plant proteins with heavy metals based on a molecular dynamics simulation technology. According to the method, molecular dynamics simulation software is used for simulating and analyzing chelation of protein and heavy metal ions. Modeling is carried out according to protein required by simulation, a proper force field and a proper solvent are selected, reasonable simulation parameters and steps are set, statistical analysis is carried out on simulation results, and the interaction force between the protein and metal ions is mainly researched. In addition, the method can accurately simulate the secondary structure, the hydrogen bond number, the energy change and the like of the protein in the chelating process, so that the chelating mechanism of the plant protein under the heavy metal stress is deeply analyzed. Compared with a traditional experimental scheme, the method has the advantages of being easy and convenient to operate, low in cost, high in precision and the like, the effect of protein in phytoremediation of heavy metal can be better understood, and wide application of plants in the field of environmental remediation is promoted.

The present application relates to a kind of mine ecological environment multilevel repair system and method, including intercepting and draining assembly, water storage assembly, drip irrigation assembly, flow assembly, intercepting and draining assembly is used to prevent soil loss and carry out large-scale water storage, water storage assembly is used to form multiple gradient water storage cavities in waterway.The repair system is repaired to ecological environment by intercepting, water storage, plant repair and other multilevel repair means, intercepting can effectively prevent the surface layer of dump site from losing a large number of guest soil, and water storage range is larger, except a small number of areas, most of the rainwater of entire dump site flows into waterway, water storage can be continuously drip irrigation to slope, effectively solve the problem that the slope water storage capacity is poor and then leads to low vegetation survival rate, also by setting first buoyancy part, when rainfall is large, make the water baffle rise, flow out narrow gap at the bottom of water baffle, so that water flow increases in narrow gap, effectively dredge.

This invention relates to the field of soil pollution control technology, specifically to a microbial-phytoremediation method for heavy metal contaminated soil in mining waste sites. It includes a supporting base layer, on which a leachate collection layer, a heavy metal passivation and isolation layer, a microbial reinforcement layer, and a plant growth layer are sequentially arranged. This invention organically integrates multi-layered structural units to form a spatially ordered structure of leachate drainage—heavy metal passivation—microbial loading—plant growth. It employs immobilized functional microbial agents and embeds multi-parameter sensing devices to establish a monitoring-feedback-control closed-loop system. Simultaneously, hyperaccumulating plants are planted and inoculated with arbuscular mycorrhizal fungi, achieving a shift from an open-loop passive mode to a closed-loop active collaborative mode, avoiding problems such as delayed remediation response, poor synergy, and insufficient long-term stability.