Heavy metal resistant nodule bacterium and method of promoting tailings area plant restoration by using same

A phytoremediation and rhizobia technology, which is applied in the fields of agriculture and environmental pollution control, can solve problems such as poor viscosity, strong water permeability, and unfavorable plant growth, so as to reduce soil erosion, promote plant growth, and improve plant stress resistance.

Active Publication Date: 2013-02-20
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the tailings area, there are many tailings, often uneven and irregular sand piles, with poor viscosity, strong water permeability, low corrosion resistance, poor water retention and fertility, and relatively low organic matter and nitrogen, phosphorus and potassium nutrients necessary for plant growth , not conducive to plant growth

Method used

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  • Heavy metal resistant nodule bacterium and method of promoting tailings area plant restoration by using same
  • Heavy metal resistant nodule bacterium and method of promoting tailings area plant restoration by using same
  • Heavy metal resistant nodule bacterium and method of promoting tailings area plant restoration by using same

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Experimental program
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Effect test

Embodiment 1

[0027] The heavy metal-resistant plant growth-promoting bacteria W33 bacterial strain of the present invention is isolated and purified from the rhizosphere soil of Leptochloa chinensis (Linn.) Nees in Longshan, Nanjing, and the isolation and identification method is as follows:

[0028] Use sterile tweezers to take the rhizosphere soil that is closely attached to the soil of about 2mm on the surface of the root system, weigh 10g, place it in a triangular flask filled with 90ml of sterile water, shake it fully for 30min, let it stand for 10min, take 0.1ml of the suspension, and use 10-fold serial dilution method, spread on YMA plates containing 0.004% Congo red (sucrose 10.0g, MgSO 4 ·7H 2 O 0.2g, K 2 HPO 4 0.5g, NaCl 0.1g, CaCO 3 3.0g, yeast extract 5g, water 1000mL, agar 20g), culture at 28°C for 3 days, pick a single colony and streak it on a YMA plate for purification and save it. Strains were inoculated in the absence of CaCO 3 In the YMA culture medium, shake cult...

Embodiment 2

[0030] Streak inoculation of W33 (CCTCC NO: M 2012357) in YMA medium containing different concentrations of heavy metals, culture at 30°C for 3 days, and observe its growth and growth conditions. The results are shown in Table 1. W33 strain can tolerate a variety of heavy metals (minimum inhibitory concentrations of Cu, Pb, and Cd are 0.8, 3.6, and 0.4 mM, respectively).

[0031] The growth situation of table 1 bacterial strain on the YMA medium containing different heavy metal concentrations

[0032]

[0033] In the above table, "+" means growth, and "-" means no growth.

Embodiment 3

[0035] Siderophores were determined according to the method of Wang Pingping. W33 (CCTCC NO: M 2012357) was inoculated in YMA liquid medium, and shaken at 150 rpm at 30°C for 48 hours. The fermentation broth was centrifuged at 12000rpm for 5min, the supernatant was taken and mixed with an equal volume of CAS detection solution, and the absorbance value (A) at the wavelength of 630nm was measured after 1h of color development, and deionized water was used as a control to adjust to zero. In addition, the same volume of sterile YMA liquid medium and CAS detection solution were mixed thoroughly, and the absorbance value was measured in the same way as the reference value (Ar). A / Ar values ​​< 1 can be considered as high siderophore producers. The results showed that the A / Ar of W33 (CCTCC NO: M 2012357) fermentation broth was 0.4, and siderophore was highly produced.

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Abstract

The invention belongs to the technical field of agriculture and environmental pollution improvement and relates to a heavy metal resistant plant endogenous nodule bacterium and application thereof. The heavy metal resistant nodule bacterium is preserved at the China center for type culture collection (CCTCC) with the preservation date to be 2012/9/18 and culture preservation number to be CCTCC NO:M2012357. The bacterial strain liquid preparation contains more than a billion of effective living bacteria per milliliter, and the bacterial strain solid preparation contains 0.2 billion of effective living bacteria per gram. The heavy metal resistant nodule bacterium strain can secrete plant hormones indole acetic acid (IAA) and siderophores, and resists heavy metal copper and cadmium. When plants are planted in moist soil or tailings containing heavy metal and bioremediation preparation is inoculated, the heavy metal resistant nodule bacterium can promote plant growth and absorbs heavy metal copper, can improve plant restoration efficiency, can fix sandy soil and reduces water and soil loss.

Description

technical field [0001] The invention belongs to the technical field of agriculture and environmental pollution control, and relates to a heavy metal-resistant rhizobia and a method for promoting plant growth in tailings areas. Background technique [0002] The mining and smelting process has brought about the destruction of the ecological landscape, the deterioration of the soil structure, the lack of nutrients and water, and the sharp decline in biodiversity. The waste generated (tailings sand, ore, etc.) Timely recovery, severe soil erosion and heavy metal pollution and many other ecological and environmental problems have led to serious damage to the land ecosystem in the mining area. According to reports, since the 1990s, the ecological recovery rate of mine abandoned land in my country is only 8.75%. Therefore, strengthening the governance and vegetation restoration of mining wasteland has become one of the urgent tasks that our country is currently facing. [0003] I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10B09C1/00C12R1/41
CPCB09C1/105
Inventor 何琳燕盛下放黄智李娅胡志伟
Owner NANJING AGRICULTURAL UNIVERSITY
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