The invention discloses a bipolaris yamadae (Y.Nisik.) shoemaker and a screening and identification method and application thereof. The bipolaris yamadae (Y.Nisik.) shoemaker (HXDC-1-2) has been deposited in the China General
Microbiological Culture Collection Center with the accession number CGMCC No.17778. The bipolaris yamadae (Y.Nisik.) shoemaker is applied to biological weeding when the conidia concentration is 102 conidia / ml or more, can be safely applied to
crop fields of rice, corn, soybeans, peanuts, sesame, millet,
broad beans, peas, rape, vegetables, flowers, traditional Chinese medicinal materials and fruit trees, and is used for controlling gramineous weeds, such as barnyard grass,
semen euphorbiae, herba digitariae, herba setariae viridis,
eleusine indica, leptochloa panicea,wild oat, Aleppo grass and microstegium vimineum, annular nutgrass flatsedge and part of broad-leaf weeds; and the
control effect can reach 80% or more. The bipolaris yamadae (Y.Nisik.) shoemaker, serving as a biological herbicide, has the advantages of being good in weeding effect, low in usage amount, high in safety to crops, low in cost, environment-friendly, free of a residue and free of
pollution.