Patents
Literature
1 results about "Poa annua" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Poa annua, or annual meadow grass (known in America more commonly as annual bluegrass or simply poa), is a widespread low-growing turfgrass in temperate climates. Though P. annua is commonly considered a solely annual plant due to its name, perennial bio-types do exist. Poa (πόα) is Greek for "fodder". It is one of the sweetest grasses for green fodder, but less useful than hay. This grass may have originated as a hybrid between Poa supina and Poa infirma.
Methods for improving genetic transformation efficiency of high altitude poa pratensis
The application discloses a method for improving genetic transformation rate of high-altitude meadow grass, comprising the following steps: (1) taking mature seeds of high-altitude meadow grass as explants to induce callus, and obtaining the callus; (2) adding a surfactant Silwet L-77 with a mass fraction of 3‰ of the bacterial liquid to the agrobacterium bacterial liquid carrying a hygromycin resistance gene, and obtaining a culture bacterial liquid; (3) placing the regenerated plant in a screening culture medium containing 40 mg / L of hygromycin resistance for culture, screening the survived plants, and obtaining the genetically transformed plants. The method can improve the transformation efficiency of the high-altitude meadow grass by about 4.54 times through adding the surfactant in the genetic transformation process and cooperating with the screening of the obtained differentiation culture medium.
Owner:KUNMING INST OF BOTANY CHINESE ACAD OF SCI