4 results about "Poa annua" patented technology

The application discloses a method for improving genetic transformation rate of high-altitude meadow grass, comprising the following steps: (1) taking mature seeds of high-altitude meadow grass as explants to induce callus, and obtaining the callus; (2) adding a surfactant Silwet L-77 with a mass fraction of 3‰ of the bacterial liquid to the agrobacterium bacterial liquid carrying a hygromycin resistance gene, and obtaining a culture bacterial liquid; (3) placing the regenerated plant in a screening culture medium containing 40 mg / L of hygromycin resistance for culture, screening the survived plants, and obtaining the genetically transformed plants. The method can improve the transformation efficiency of the high-altitude meadow grass by about 4.54 times through adding the surfactant in the genetic transformation process and cooperating with the screening of the obtained differentiation culture medium.

The invention relates to the technical field of propagation regulation and control, and provides a big data-based intelligent optimization method for the rootstock asexual propagation of bluegrass, which is applied to a rootstock asexual propagation production scene and runs in a closed-loop regulation and control system consisting of field sensing equipment, an edge computing gateway, a central intelligent decision-making server and water, fertilizer and agricultural machinery execution equipment. According to the method, a propagation state vector is constructed based on multi-source field perception data, a rhizome asexual propagation regulation process is modeled into a long-term time sequence decision problem with resources and security constraints, a prefix-guided reinforcement learning training mechanism is introduced by screening historical departure strategy trajectories, and the method is suitable for the field asexual propagation regulation process on the premise of meeting equipment performability and agronomic security constraints. Collaborative optimization of irrigation, fertilization and agricultural machinery operation is realized, so that the stability of vegetative propagation of the roots and stems of the bluegrass and the resource utilization efficiency are improved.

The invention relates to the technical field of agricultural breeding, in particular to a meadow grass-endophytic fungus symbiont construction method which comprises the following steps: culturing meadow grass aseptic seedlings; purifying and culturing an LE3 endophytic fungus strain; inoculating the LE3 endophytic fungi to the sterile seedlings of the poa pratensis; transplanting the inoculated seedlings; carrying out endophytic fungus detection on the inoculated seedlings; growing in a field: transplanting the seedlings with bacteria to the field. When the symbiont is constructed, an LE3 strain which only generates insect-resistant alkaloid and does not generate alkaloid harmful to livestock is selected. The agronomic characters of the obtained symbiont are remarkably improved compared with those of a control group. Wherein after the host plant is infected by the LE3 strain, the plant height, stem diameter, leaf length, leaf thickness, potassium element content and soluble sugar content of the host plant are obviously increased. Wherein the plant height, the stem diameter, the leaf length and the leaf thickness are respectively increased by 14.18%, 26.18%, 12.16% and 5.06%, and the potassium content and the soluble sugar content are respectively increased by 90.49% and 89.00%.

A method is disclosed for using simazine to control and reduce the formation of flowers and seed heads in turfgrasses, including cool-season turfgrasses, and to suppress the growth of annual bluegrass (Poa annua). The method comprises applying an effective dose of simazine to the turfgrasses. A method is also disclosed for enhancing the color and quality of cool-season turfgrasses as a result of a penox slam treatment applied to turfgrasses, including cool-season turfgrasses. The method comprises applying an effective dose of simazine to the turfgrasses. A method for selectively controlling barnyard grass (Paspalum vaginatum) in turfgrasses, including Bermuda grass, is further disclosed, the method comprising applying an effective dose of simazine to the turfgrasses.