The invention provides a weeding composition containing saflufenacil, glufosinate ammonium and quizalofop-p-ethyl. The weeding composition comprises the following active components saflufenacil, glufosinate ammonium and quizalofop-p-ethyl according to a mass ratio 1-50:0.1-20:0.1-20; the mass of the active components in the preparation is 1-60% of the total mass of the whole preparation, and the rest components are auxiliary components which are allowed to be used and accepted in pesticide; the dosage forms of the weeding composition comprise missible oil, suspending agent, wettable powder, water dispersible granule, emulsion in water, micron emulsion, particulate agent, microcapsule, and aqueous solution. The components can be used for effectively controlling main weeds in rice fields, such as barnyard grass, caper euphorbia seeds, crab grass, goosegrass herb and the like as well as winter wheat weeds, such as Alopecurus aequalis, Avena fatua, Polypogon fugax, Poa annua, Sclerochloa dura, Puccinellia distans, Lolium multiflorum, Lolium temulentum, Bromus japonicas, Phleum paniculatum, Aegilops tauschii, Beckmannia syzigachne, Mesembryanthemum crystallinum, shepherd's purse, Descurainia sophia, Malachium aquaticum, and the like.