The invention discloses a method for regulating and controlling drought stress festuca arundinacea protection enzyme and malondialdehyde by using graphene oxide and waste lawn nursery strip. Accordingto the method, a plastic flowerpot is mainly adopted and filled with campus soil, seeds are laid, the moderate stress is set to be 50%-60% (w / w), and the severe stress is 30%-40%. The method comprises the following steps: selecting waste as a carrier material of the lawn nursery strip, soaking for 10 minutes by using 1% hydrogen peroxide solution, taking out and drying, using waste newspaper as the lower layer of the lawn nursery strip planting building carrier, using waste gauze as an upper layer, uniformly spreading 1g of festuca arundinacea seeds in each lawn nursery strip, then uniformlyspreading the graphene oxide, wherein the use amount of the graphene oxide is 0.2-0.4 mg / g. The purpose of the invention is to compare the differences of influences on the growth and the physiologicalecology of the festuca arundinacea by the three plant buildings ways respectively including the lawn nursery strip, the graphene oxide and the lawn nursery strip loaded with the graphene oxide, the effects of lawn nursery strip and the graphene oxide on the lawn drought stress are discussed, and a technical support is provided for planting the high-quality lawn for the drought stress.