92 results about "Protective enzymes" patented technology

This invention relates to isolated or recombinant N-CBZ-deprotecting enzyme polypeptides that catalyze the removal of CBZ from CBZ-protected amino acids and alcohols, and variants, modifications, and fragments thereof. Also related are isolated nucleic acids encoding N-CBZ-deprotecting enzyme polypeptides, and variants, modifications, and fragments thereof, as well as vectors and host cells comprising these nucleic acids. The invention also relates to methods of obtaining isolated nucleic acids, polypeptides, and antibodies, and methods of using polypeptides in various reactions for industrial or pharmaceutical applications.

The invention relates to B. vallismortis HSB-2 and application thereof. The preservation number of the B. vallismortis HSB-2 is CGMCC No.15305, and the sequence of 16S rDNA is shown as SEQ.ID.NO.1. The invention further relates to the application of the B. vallismortis HSB-2 to preparation of bacterial fertilizer for relieving the successive cropping obstacle of apples. The B. vallismortis HSB-2 has an obvious antagonistic effect on four types of Fusarium fusarium pathogenic bacteria causing the successive cropping obstacle of the apples; the bacterial fertilizer manufactured through the B. vallismortis HSB-2 can promote growing of the overground parts and the underground parts of Malus malus hupehensis seedlings, and at the rhizosphere, colonization can be stabilized, and growing of the four types of Fusarium fusarium pathogenic bacteria of solani, proliferatum, verticillioides and oxysporum, causing the successive cropping obstacle of the apples, in soil can be remarkably restrained;and activity of soil urease, phosphatase, sucrase and root system protective enzymes, namely SOD, POD and CAT can be remarkably improved, and the successive cropping obstacle can be relieved.

The invention provides a method for cultivating wheat seedlings by using nitrogen stress nutrient solution. The method comprises the following steps of: repeatedly soaking wheat seeds in nitrogen stress solution until the wheat seeds sprout and grow to the height of between 5 and 20cm; and accumulating active oxygen in cells of the wheat seedlings by nitrogen stress to increase the quantity and activity of protective enzyme and enrich a polyphenolic substance. In order to prevent excessive active oxygen from damaging the cells, a method for alternating repeated and short-term nitrogen stress and nutrient solution cultivation is adopted to make the active oxygen slowly generated. By the method, more superoxide dismutase, catalase, peroxidase and the polyphenolic substance can be obtained, the yield of the wheat seedlings is improved, and new species of wheat seedlings rich in antioxidants are produced.

The invention discloses application of remedied domestic waste composts in adjusting the activities of antioxidant enzymes of lawn plants; wherein, the remedied waste composts refer to compost matrixes obtained by eluting the composts with water based on the weight ratio of 1:1-3; the activities of the antioxidant enzymes comprise superoxide dismutase, peroxide dismutase and catalase. The technical effects of the invention show that the method of planting lawn plants on the remedied waste composts not only can enhance the activities of the protective enzyme systems of the lawn plants but also can improve the capabilities of the lawn plants in resisting unfavorable growth environment obviously, therefore, the technology not only finds a way for scientifically and effectively planting environment-friendly lawns on the matrixes of the eluted compost, but also can achieve the purpose of reducing the environment pollution caused by the domestic waste composts and provide technical support for applying the remedied waste composts to the lawn planting system.

The invention discloses a Bacillus velezensis strain NH-1 capable of preventing cucumber fusarium wilt. The Bacillus velezensis strain NH-1 is preserved at China Center for Type Culture Collection, and the preservation number is CCTCC NO:M 2017336. The invention also discloses a microorganism bacteria preparation capable of preventing cucumber fusarium wilt. The active ingredient of the microorganism bacteria preparation is the Bacillus velezensis strain NH-1. The microorganism bacteria preparation is high in preventing and controlling efficiency, is safe, is economical, and is friendly to theenvironment; effect lasts long; and cucumber fusarium wilt prevention is realized via increasing the activity of protective enzymes in cucumber.

The invention relates to a method for planting lawn plants by combining rare earth cerium with mixed culture matrices. The method comprises the step of sowing seeds of lawn plants on mixed culture matrices for lawn planting, wherein 1.5-2kg of mixed culture matrix soil contains 200-800mg/Kg of rare earth cerium, and the mixed culture matrices refer to soil and municipal solid waste compost in a ratio of parts by weight of 4: (1-2). By applying a certain amount of rare earth cerium, the activities of protective enzymes of the lawn plants can be obviously improved, and the defence capability of the protective enzyme system can be enhanced, thus improving the drought resistance of the lawn plants. Efficient and low-cost operation modes in large-area municipal lawn planting are expected to be achieved through the application of the technology.

The chelating state micronutrient fertilizer with the insecticidal function comprises trace elements and chelating agents and also comprises botanical pesticides and dispersion agents; the chelating state micronutrient fertilizer is shaped like powder; chelating agents are selected from tetrasodium iminodisuccinate salt and or EDTA (Ethylene Diamine Tetraacetic Acid); the botanical pesticides comprise Cynanchum komarovii Al. extracts and also comprise Stellera chamaejasme extracts and Ageratina adenophora extracts. According to the chelating state micronutrient fertilizer with the insecticidal function, the trace elements are added, trace elements and other nutrients brought by a plant source which serves as the pesticides are contained, the trace elements and the other nutrients can be chelated into the chelating state micronutrient fertilizer, the botanical pesticides are also contained, and accordingly the formula is reasonable, the synergistic effect is obvious, the utilization rate of effective components is high, the pest killing effect is good through due to the double insecticidal mechanism for damaging protective enzyme systems and nervous systems of nematodes and the fumigation action of multiple ingredients, and the botanical pesticides are free of pest resistance. The invention also relates to a production method of the chelating state micronutrient fertilizer with the insecticidal function.

A method for identifying modulators of the Keap1-NrG-ARE pathway is described. In particular, an assay is described that identifies molecules that inhibit the binding of a labeled Nrf2 peptide with the kelch domain of the Keap1 protein. Molecules that inhibit the binding are activators of the Keap 1-Nrf2-ARE pathway. Activation of the Keap 1-Nrf2-ARE pathway may result in an increased accumulation of Nrf2 and the subsequent induction of protective enzymes, for example, the phase 2 detoxification enzymes. Activators of the Keap1-NrG-ARE pathway are useful for combating oxidative stress-related disorders, such as those associated with cancer, emphysema, Huntington's disease, light-induced retinal damage, and stroke.

The invention discloses a maize breeding method. A BT insecticidal toxic protein gene, a bar gene and maize dwarf mosaic virus coat protein gene MDMVCPDNA are introduced into embryogenic maize calluses through particle bombardment, so that seeds with excellent insect resistance, weed resistance, disease resistance and drought tolerance are obtained; through seed soaking treatment by seed-soaking liquid, germination of seeds is promoted, the respiration rate of seedlings is increased, the root activity of the seedlings is improved, and the resistance of both the seeds and the seedlings is enhanced; through drip irrigation of a drought-resistant nutrient and by using anti-oxidation substances of phenolic acids, the number and activity of the protective enzymes in the plants are improved, the content of polyphenol substances is increased, the content of anti-oxidation substances in the crop is increased and the drought resistance of the crop is enhanced; meanwhile, since the content of the anti-oxidation substances in the crop is increased, the disease resistance, UV radiation resistance and the like of the crop are improved; since monopotassium phosphate and nitrogen are added into the phenol substances, nutrient elements supplied to the crop are increased.

The present invention is directed to restricted access media (RAM), methods for preparing restricted access media, and kits for preparing restricted access media that contain protected ligand binding agents or protected enzymes. Certain RAM provided contain a plurality of protected regions of the support that contain ligand binding agents that are protected by blocking agents. Certain RAM provided contain a plurality of protected regions of the support that contain unbound ligand binding agents or enzymes that are retained in the protected regions by a capping agent. Methods of making the RAM of the invention and associated kits are also provided

The invention discloses a preparation method of nutrient-balanced fertilizer special for tea trees. The preparation method comprises the following steps that peanut meal, humic acid, corncob powder, coconut bran, castor meal and water are mixed and fermented to obtain a prefabricated material; the prefabricated material and a modified dispersant are mixed uniformly, ammonium nitrate, triple superphosphate, kiln dust potash fertilizer, zeolite powder, sepiolite powder, EDTA chelate zinc, humic acid copper and EDTA chelate iron are mixed uniformly, and granulating, sieving and drying are conducted to obtain the nutrient-balanced fertilizer special for the tea trees. Not only is the fertilizer special for the tea trees extremely good in nutrient substance slow-release effect, the demands of the tea trees during the whole growth period are met simply by applying the fertilizer once, but also harmful bacteria can be effectively inhibited, the soil fertility is enhanced, the growth capability of the tea trees is stimulated, the protective enzyme activity of tea leaves can be effectively improved, the disease resistance of the tea trees is improved, at the same time, the crumb structure of soil can be reformed, the soil is looser, and the fertilizer is conducive to the growth of the tea trees.

The invention discloses an application of eutrophic water in the regulation of antioxidase activity of lawn plants, wherein the eutrophic water is the eutrophic water with 25 percent-75 percent of the original concentration; and the eutrophic water has TN of 9.7 mg/L-13.1mg/L, TP of 0.6mg/L -0.9mg/L and pH value of 8.12-8.99, wherein the activity of antioxidase comprises of superoxide dismutase, peroxide dismutase and catalase. Results show that the diluted eutrophic water is applied to irrigation to reinforce the activity of a protective enzyme system of lawn plants, thereby being capable of obviously improving the resistance ability of the lawn plants to adverse growing environment and providing technical support for the application of the eutrophic water in a lawn plant establishment system.

The invention discloses a method for improving winter resistance of turf by adopting household garbage compost offscourings. The ryegrass seedlings cultivated by compost and cold-hardened in cold environment with gradually decreased temperature have no obvious difference from the seedlings in the control group in indexes such as protective enzyme, proline and conductivity, but are slightly shorter. The aboveground biomass of the cold-hardened ryegrass seedlings cultivated by compost offscourings is reduced by 26.74% compared with that of the seedlings in the control group and is reduced by 23.21% compared with that of the seedlings with relatively poorer matix nutrition in the filter paper group. The final technical effect shows that the cold-hardened turf can fully resist winter in the winter environment of not less than 10 DEG C below zero in the outdoor balcony without being provided with a heating facility, and the aim of storing the turf in winter and producing all year around is achieved.

The invention discloses a method for improving the salt resistance of tall fescue by virtue of an air-dried sludge microbial agent. The microbial agents comprise Pseudomonas stutzeri + Trichoderma reesei + 100-fold dilution of Streptomyces flavus or Pseudomonas stutzeri + Trichoderma reesei + 200-fold dilution of Streptomyces flavus. The experimental results show that the plant height, biomass and chlorophyll content of tall fescue can be significantly improved, the contents of malonaldehyde and proline are lowered and the protective enzyme activity of tall fescue is enhanced by applying sludge complex microbial agents. On the whole, the microbial agents exhibit better effects in low fertility and the salinized soil, which fully shows the potential applications of microbial agents in the saline-alkali soil improvement.

The invention relates to a preparation method and application of total nutrient microorganism biocontrol organic fertilizer. The preparation method of the total nutrient microorganism biocontrol organic fertilizer comprises the following steps: firstly, carrying out plate cultivation on trichoderma pseudokoningii TRIPI and rhizopus nigricans RN-9; secondly, carrying out PDB liquid culture on TRIPI and RN-9 hypha blocks; thirdly, carrying out fermentation culture on a liquid culture solution, so that fermentation liquor is obtained; fourthly, inoculating the fermentation liquor to farmyard manure raw materials, uniformly mixing, stockpiling and fermenting, so that a fermented material is obtained; fifthly, adding other ingredients into the fermented material, and uniformly mixing, so that the total nutrient microorganism biocontrol organic fertilizer is obtained. The prepared total nutrient microorganism biocontrol organic fertilizer has high inhibition ratio on pathogenic bacteria and high relative control efficiency on fusarium wilt of cucumber; compared with biocontrol organic fertilizer prepared by adopting single strain fermentation liquor, content of pathogenic bacteria in cucumber rhizosphere soil, cucumber root system and cucumber stalk is low, and cucumber blade protective enzyme is quick in response, high in activity and long in duration of time.

The invention provides a method for relieving drought stress of Actinidia chinensis fruit trees. The method comprises the following step of irrigating melatonin at the concentration of 50 to 200 umol/L in seedlings of the Actinidia chinensis for one time every other 2 days, totally for four times, wherein the irrigation amount is 200 ml. By the method, the relative water content of leaves of the seedlings of wild Actinidia chinensis in the same year under the drought stress can be obviously increased, damage of malonaldehyde to cytomembrane is reduced, the relative membrane permeability is reduced, proline concentration and soluble sugar concentration are reduced, degradation of soluble protein is inhibited, high activity of protective enzyme is maintained, the plants can adapt to the drought environment better and the drought resistance of the plants is enhanced.

The invention discloses a method for improving protective enzyme activity of turfgrass plants by utilizing air-dried sludge. Air-dried sludge combination substrates comprise sludge and soil with, by weight, the part ratio of 1: (3-7). Experiments show that air-dried sludge can facilitate the growth of the turfgrass plants evidently. When festuca arundinacea and lolium perenne are planted in conditions of soil and sludge combination substrates with the part ratios of 1:7, 3:13 and 1:3 respectively, the activities of turfgrass protective enzymes of POD (peroxidase) and CAT (catalase) are improved evidently and show significant differences with control groups. Therefore, the dewatered sludge of a sewage treatment plant can be directly applied to the cultivation of the turfgrass plants after being air-dried naturally to improve the activities of the turfgrass protective enzymes activity.

The invention discloses a method for improving protective enzyme activities of salt-stressed turfgrass by adopting an enhanced salt-tolerant microbial agent. The enhanced salt-tolerant microbial agent comprises enhanced bacillus subtilis, enhanced lysine bacillus and enhanced penicillium chrysogenum in a weight part ratio of 1 to 1 to 1, wherein enhanced bacillus subtilis, enhanced lysine bacillus and enhanced penicillium chrysogenum are salt-tolerant mixed microbial flora obtained by carrying out domestication step by step with a method for increasing the salt concentration step by step. The invention further discloses an application of the enhanced salt-tolerant microbial agent in improvement of the protective enzyme activities of salt-stressed turfgrass festuca arundinacea L. Experimental results show that the compound enhanced microbial agent can conduce to effectively alleviating the harms of saline-alkali soil to the turfgrass; screening and culturing corresponding enhanced salt-tolerant compost microbial strains and carrying out artificial inoculation is a new way for improving the salt tolerance of the turfgrass.

The invention relates to a method for aridity of ryegrass by using rare earth lanthanum and particularly relates to application to increasing activity of protective enzyme of ryegrass and stress resistance of ryegrass by using the rare earth lanthanum. The method comprises the following steps of: sowing seeds of ryegrass on garden soil subjected to lawn establishment, wherein every 100-200g of garden soil contains 200mg/Kg-800mg/Kg of lanthanum nitrate; and at the initial stage of sowing, performing watering thoroughly so as to guarantee the smooth germination of the seeds and the initial-stage growth of young seedlings. When certain amount of rare earth is used, the activity of the protective enzyme of the ryegrass is remarkably increased, the defense capability of a protective enzyme system is strengthened, so that the drought resistance of the lawn plants is improved; in addition, the application of the technology is hopeful for the realization of the efficient and low-cost operation mode for the large-area city lawn establishment.

The invention discloses a method for improving protective enzyme activity of grass of a water stress lawn by adopting a reinforced drought-resistant microbial agent. The reinforced drought-resistant microbial agent comprises the following components of reinforced bacillus cereuses, reinforced lysine bacillus and reinforced rhodotorula glutinis, with the weight ratio of 1: 1: 1. The drought resistance and the reinforcement of a mixed microbial population are performed by adopting the method of gradually increasing the concentration of PEG6000. The invention further discloses the application of the reinforced drought-resistant microbial agent in the field of improving the protective enzyme activity of festuca arundinacea of the lawn grass under the drought stress. Experiments prove that under the drought stress, the protective enzyme activity of the festuca arundinacea can be obviously improved, so that damage caused by the drought stress to the festuca arundinacea is alleviated, and the improvement of the drought-resistance of the lawn grass is facilitated.

The invention provides a method for promoting seed germination through regulating and controlling the content of zinc in soaking liquid and relates to a seed treatment method. The method is implemented by the following steps: a, soaking crop seeds with disinfection fluid with a volume concentration of 5-15 percent for 20-50 minutes and thoroughly washing with deionized water to obtain a disinfected crop seeds; b, soaking the disinfected crop seeds obtained in the step a with chelated zinc of 0.1-3 mmol.L<-1> for 16-40 h, so as to complete seed treatment. According to the method for promoting the seed germination through regulating and controlling the content of zinc in the soaking liquid, provided by the invention, the absorption and the utilization of zinc of a plant are facilitated, and meanwhile, antagonistic action among elements can also be avoided; through regulating and controlling the content of zinc in the soaking liquid, the promoting effect of zinc on the seed germination is judged from the perspective of seed germination, growth, and changes in protective enzyme activity. Through the seed soaking treatment with zinc, the situation that the plant growth is affected due to soil zinc deficiency is improved while the soil pollution caused by excessive application of zinc fertilizers in a farmland is avoided.