19 results about "Protective enzymes" patented technology

The application relates to the technical field of wastewater treatment, and particularly discloses a degradation method and application of microplastics in aquaculture wastewater. The degradation method comprises the steps of preparation of a mesoporous structure magnetic immobilized material, pretreatment, adsorption-enzymolysis, microbial degradation, magnetic separation, precipitation and the like. The shell layer and the mesoporous structure of the mesoporous material are used to efficiently immobilize composite enzymes, so that enzyme loss is avoided, and the composite enzymes are used for targeted degradation of PET, PE and PP, and the total microplastic removal rate is more than 94.5%. The composite heat-resistant bacteria relay enzymolysis product is deepened and mineralized, and a phased treatment agent is matched to enhance mass transfer, so that the microbial activity is increased by 3 times. The mesoporous material is protected by the shell layer, the enzyme activity retention rate is more than 85%, the effluent COD is less than or equal to 50 mg / L, meets the discharge requirements, is suitable for microplastic pollution treatment in multiple scenes, and has the advantages of high efficiency, economy and environmental friendliness.

The present application relates to a kind of metal-organic framework mediated multi-enzyme nanoparticles and its application, especially the method for alpha-amylase detection. By detecting product absorbance, the quantitative detection of alpha-amylase activity is realized. According to the nature of MFM analog enzyme and its ability as natural enzyme carrier, Agl@MFM@Gox@MFM is prepared, compared with natural enzyme, the composite material has strong resistance, can successfully protect the activity of enzyme. Based on the cascade catalytic system of multi-enzyme nanoparticles Agl@MFM@Gox@MFM, a biosensor is first synthesized and established for detecting alpha-amylase, successfully combines chemical catalyst with natural enzyme, has wide detection linear range (5-500 U / L) while has excellent specificity to alpha-amylase. Benefited from the advantages of low cost, easy preparation and excellent stability, the colorimetric sensor has been successfully used to determine the activity of alpha-amylase in koji.

The invention provides a Tibetan tea insoluble dietary fiber and an extraction method thereof, Tibetan tea powder is subjected to freezing ball milling-low temperature plasma gas phase modification synergistic pretreatment to destroy the compact structure of cell walls and improve the hydrophilicity of the tea powder, then multi-enzyme sequential enzymolysis is carried out under the assistance of an alkyl glycoside-chitosan / graphene quantum dot compound, and the Tibetan tea insoluble dietary fiber is obtained. In the compound, the graphene quantum dots can permeate and expand pores, the alkyl glycoside can expand a fiber structure and protect enzyme activity, and the chitosan can adsorb impurities, so that the inhibition effect of polyphenol on enzyme is effectively overcome, and the enzymolysis efficiency and the impurity removal effect are improved. Through the synergistic effect of physical wall breaking and functional compound auxiliary enzymolysis, the extraction purity and yield of the insoluble dietary fibers of the Tibetan tea are remarkably improved, and a new way is provided for high-value utilization of Tibetan tea resources.

The invention relates to the technical field of agricultural production, and particularly discloses a humic acid biochar composite preparation and a preparation method and application thereof, and the humic acid biochar composite preparation is composed of 120-480 kg / mu of biochar and 49-51 L / mu of 100-400 mg / L of humic acid. The humic acid biochar composite preparation provided by the invention can significantly improve the activity of millet protective enzyme, improve the physical and chemical properties of soil, enhance the enzyme activity of soil, enhance the stress resistance of millet and the fertility of millet planting soil, and promote the growth and development of millet seedlings.

The application provides a high-efficiency detergent and a preparation method thereof, and relates to the technical field of daily chemical products.The detergent comprises microencapsulated composite enzymes, chelating agents, surfactants, additives and water; the active ingredients of the microencapsulated composite enzymes are composed of lipase, protease and amylase, and are prepared by sodium alginate-chitosan microencapsulation embedding, so that the enzyme activity is effectively protected, the stability of the enzymes in the detergent system is improved, and the targeted decomposition of composite stains is realized; the chelating agents are composed of tetrasodium iminodisuccinate and maleic acid-acrylic acid copolymer sodium salt, and can synergistically improve the hard water resistance and the stain removal efficiency; the surfactants are composed of sodium fatty acid methyl ester sulfonate, alkyl polyglycoside and cocamide propyl betaine, and have strong emulsifying and stain removing power and mildness.The detergent is suitable for cleaning tableware and fruits and vegetables, and has the characteristics of high stability, strong stain removing power, easy rinsing, mildness and environmental protection.

The utility model discloses an anti-pollution pesticide residue detection card, which belongs to the field of pesticide residue detection and comprises paper, a crease is arranged in the middle of the paper, a supporting plate is fixedly connected to one side of the paper, an enzyme sheet is arranged on the supporting plate, a first film is arranged at the upper end of the enzyme sheet, the first film is adhered to the top of the supporting plate, and the first film covers the enzyme sheet. The first film is larger than the supporting plate. A groove is formed in the other side of the paper, and a second film is arranged at the upper end of the annular ring, pasted to the annular ring and used for blocking an upper end opening of the annular ring. According to the pesticide residue detection card disclosed by the invention, the first film is adhered to the top of the supporting plate, covers the enzyme sheet and is used for protecting the enzyme sheet from external pollution; the second film is adhered to the annular ring to block the upper port of the annular ring, so that the substrate sheet and a possible reaction area are protected from external pollution, the detection accuracy is improved, the quality guarantee period of the detection card is prolonged, and the use cost is reduced.

The invention provides application of beauveria bassiana HFBb18 in preparation of a green peach aphid biological control entomopathogenic fungus agent, and belongs to the technical field of agricultural biology. The inventor acquires and identifies three new strains of beauveria bassiana from the field, then selects three strains of beauveria bassiana preserved in a laboratory, and finds that the newly found HFBb18 strain has high toxicity to green peach aphids through indoor toxicity determination. On the basis, the influence of infection of the HFBb18 strain on the activities of detoxifying enzyme and protecting enzyme of green peach aphid is determined. Furthermore, the wettable powder taking the HFBb18 strain conidia as a main active ingredient is prepared, the potting pesticide effect test control effect of the wettable powder on green peach aphids is defined, and a foundation is laid for development and application of a novel fungal aphid killing agent.

The utility model relates to the technical field of filtering devices, and particularly discloses a multi-stage fine filtering device for a biological enzyme preparation, which comprises a pre-filter, a coarse filter and a fine filter which are arranged in sequence, a steam filter is arranged between the coarse filter and the fine filter; wherein each of the pre-filter, the coarse filter and the fine filter comprises a base, a cover body arranged at the top of the base, a locking piece for fixing the cover body at the top of the base and a microporous membrane filter element mounted at the top of the base and arranged in the cover body, and an input port communicated with an input cavity in the base is formed in the top of the base; according to the utility model, the series design of pre-filtration, rough filtration, steam sterilization and fine filtration reduces the impurity load step by step, the steam filter is arranged between the rough filtration and the fine filtration, and the sterilized liquid is subjected to fine filtration, so that the filtration precision is improved, the enzyme activity is prevented from being damaged due to incomplete sterilization or high temperature, the sterilization and filtration are synergistic, the sterility is ensured, and the enzyme activity is protected at the same time.

The application provides a kind of cluster root fungus agent for improving the cold resistance of durian and a preparation method thereof, and belongs to the technical field of microbial inoculant.The preparation method of the cluster root fungus agent comprises the following steps: collecting subfrigid zone plant rhizosphere soil, and screening cluster root fungus spores in the soil;inoculating the cluster root fungus spores into sand mixed culture medium, then planting white clover or ryegrass and other plants to carry out enrichment culture and obtain culture;mixing the culture with cutting substrate, and cutting one-year-old durian branches to carry out rooting culture and first low-temperature culture;storing the root system of cutting seedlings in cold storage, crushing, mixing with cutting substrate, cutting one-year-old durian branches to carry out second low-temperature culture, and collecting the root system of cutting seedlings and rhizosphere soil to obtain the cluster root fungus agent.The cluster root fungus agent can induce durian to produce low-temperature resistant protective enzymes, thereby improving the cold resistance of durian and providing a new idea for cultivating durian cold-resistant varieties.

The invention provides a PCR (Polymerase Chain Reaction) premixed solution with high sensitivity and rapid amplification, a PCR amplification kit and a PCR method, and relates to the technical field of biology. The PCR premixed solution provided by the invention comprises a PCR enhancer with specific composition, glycerol and TMAC (tetramethylammonium chloride) can reduce non-specific amplification of primers, and the combined use effect of the glycerol and the TMAC is superior to that of any single component; glycine betaine and gelatin have the effect of enhancing sensitivity, and the combined use effect of glycine betaine and gelatin is better; glycerol and betaine can increase the duration of tolerating rapid heating and cooling, have a superposition effect, and do not have an antagonistic effect with other components at the same time; the glycerol and the betaine can obviously improve the stability of the Taq enzyme and obviously increase the temperature tolerance. Therefore, the enhancer provided by the invention can protect the activity of enzyme, promote the amplification efficiency and ensure the stability and accuracy under the condition of rapid amplification.

The application discloses a DREB transcription factor CtDREB1B of safflower and application thereof in positive regulation of drought and salt stress resistance of plants. The full-length nucleotide sequence of the gene CtDREB1B is shown as SEQ ID NO. 2, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 1. The application first clones the complete coding frame of the gene by RT-PCR, clones the gene into a pYES2 vector and transfers the gene into yeast, and finds that the pYES2::CtDREB1B yeast cells can still grow on SGR culture medium containing 1.5 M mannitol or 4 M NaCl. Then, the CtDREB1B gene is heterologously expressed into Arabidopsis thaliana. Overexpression of the CtDREB1B in the Arabidopsis thaliana can make the transgenic Arabidopsis thaliana exhibit a drought and salt tolerance phenotype, and the activities of protective enzymes such as SOD and POD are enhanced, and the expression amounts of stress resistance related genes such as AtABF3 and AtRD29A are up-regulated, indicating that the gene can simultaneously positively regulate drought and salt stress. Therefore, the gene can be applied to breeding of new plant stress resistance varieties, and in addition, the gene provides a new gene resource for plant stress resistance, and has important application prospects in breeding of salt-tolerant plants.

The invention belongs to the technical field of detection, and particularly relates to a composite electrode, a preparation method and application thereof, and a detection method of organophosphorus pesticide content. The composite electrode provided by the invention adopts the oxide with a three-dimensional ordered macroporous structure as a supporting platform, has a relatively large specific surface area and good conductivity, and effectively improves the immobilization efficiency and stability of a biological material. By loading high-dispersion noble metal, the electro-catalytic performance is further enhanced, and the sensitivity of electrochemical reaction is improved. Acetylcholinesterase (AChE) is used as a biological recognition element, and efficient detection of pesticide residues is achieved by means of the inhibition effect of AChE on organophosphorus pesticides. Due to the introduction of noble metal, the catalytic efficiency of electrochemical reaction is enhanced, and the sensitivity of the sensor is improved. Meanwhile, the three-dimensional ordered macroporous structure can effectively protect the activity of enzyme molecules and avoid inactivation of the enzyme molecules in the detection process, so that the stability and repeatability of the sensor are improved.

The invention discloses a high-activity immobilized plastic degrading enzyme preparation and application thereof, and belongs to the technical field of plastic degradation. The enzyme preparation is prepared by the following steps: by taking pinus tabulaeformis pollen as a raw material, performing acid treatment, carbonization and activation to form a hierarchical porous active pollen carrier; through lanthanum-doped magnetic nanoparticle modification and sulfydryl functional modification, the carrier is endowed with magnetic responsiveness and directional fixed sites; a three-dimensional cross-linked structure is constructed by using a triazine ring rigid conjugated monomer and a hyperbranched network, and spatial confinement immobilization of enzyme molecules is realized by combining an ultraviolet click reaction; and cutinase, lipase, cellulase, laccase and peroxidase are synergistically loaded to form a multi-enzyme catalytic system. By combining pollen-based hierarchical pore channels with a triazine ring rigid network, the enzyme loading capacity and distribution uniformity are remarkably improved, the enzyme activity retention rate of 90% or above is still kept after an enzyme preparation is recycled for 10 times through cross-linked network protection, and rapid magnetic separation is achieved through lanthanum-doped ferroferric oxide nanoparticles.

A composition for the removal of resin-injected starch from a surface, the composition comprising: an enzyme present in an amount in the range of about 15 - 30% w / w to break down the starch into water soluble units; at least one solvent in an amount in the range of about 2 - 4% w / w, effective to liquify resins present in the starch; a cofactor present in an amount in the range of about 5 - 10% w / w to function as an enhancer to assist the enzyme to break down the starch into water soluble units; at least one surfactant in an amount in the range of about 14 - 25% w / w to retain the enzyme in solution; and a bacteriocide in an amount in the range of 0.01 – 0.04% w / w, effective to protect the enzyme from bacteria.

The invention relates to the technical field of nutritious sports food, and discloses an anti-coagulation and anti-thrombus combined spintine 48k gel type nutritious sports food and a process, and the content of the food is composed of a pH-responsive enzyme active core, a temperature-sensitive slow-release liposome and an in-situ self-emulsifying thixotropic gel matrix. The preparation process comprises the following steps: respectively pre-preparing the enzyme active core and the lipidosome; preparing a gel matrix; and finally, mixing and dispersing the two components in a gel matrix at low speed and low shear force, and performing soft capsule packaging. The enzyme activity is protected through the pH responsive core, and fixed-point release of the intestinal tract is realized; body temperature pre-release of the auxiliary material is realized through the temperature-sensitive slow-release lipidosome; a three-dimensional network structure constructed by the thixotropic gel matrix effectively prevents solid particles from settling, so that the uniformity and stability of the product are ensured, and meanwhile, the self-emulsifying characteristic of the thixotropic gel matrix promotes component absorption. The technical problems that enzyme is prone to inactivation and the system is unstable are solved, and the structure and function integrated multistage time sequence release system is provided.

Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises an enzyme layer, wherein the enzyme layer comprises an enzyme and a polymer comprising polyurethane and / or polyurea segments and one or more zwitterionic repeating units. The enzyme layer protects the enzyme and prevents it from leaching from the sensing membrane into a host or deactivating.

The invention relates to the technical field of environmental functional materials and water treatment, in particular to a method for rapidly removing phenol pollutants in a water body by using a synthetase-metal organic framework composite material. The method comprises the following steps: S1, mixing horse radish peroxidase (HRP) with a polyvinylpyrrolidone (PVP) solution, a zinc salt solution and a 2-methylimidazole (HmIM) solution, reacting at room temperature for 1-3 minutes, centrifuging, washing and drying to obtain a composite material R-HRP-ZIF-L; and S2, adding the composite material and hydrogen peroxide (H2O2) into a phenol-containing water body for catalytic oxidation, and carrying out ultrafiltration separation after reaction. According to the method, the synthesis of ZIF-L is accelerated and the enzyme activity is protected by utilizing PVP, so that efficient removal of phenolic pollutants is realized. The method has the advantages of high synthesis speed, high enzyme activity, extremely low oxidant consumption, strong anti-interference capability and the like, and is suitable for deep purification of phenolic pollutants in various complex water bodies.

The invention discloses a high-temperature-resistant LAMP (Loop-Mediated Isothermal Amplification) reaction premix liquid composition independent of enzyme modification and application thereof in LAMP amplification. According to the premixed liquid composition, the reaction stability and amplification efficiency in a high-temperature environment of 60-70 DEG C are remarkably improved through an innovative formula; trehalose and mannitol are combined in a specific ratio, so that the enzyme activity is effectively protected, and the stability of a reaction system is maintained; the anti-evaporation design is innovatively introduced, the viscosity is increased by adding polyethylene glycol, and the evaporation loss rate at 70 DEG C is reduced to 3% or below by combining with a mineral oil physical barrier. The Tween-20 surfactant and the spermidine polyamine compound are added, and the reaction specificity and the template stability are synergistically improved through the action mechanisms of inhibiting protein high-temperature aggregation and reducing nucleic acid template thermal degradation respectively. The premixed liquid is suitable for the fields of pathogen detection, environmental monitoring and the like in a high-temperature environment, and has the advantages of simplicity and convenience in operation, good stability, no need of complex equipment and the like.

The invention provides a digestive tract tumor targeting composite nano material and a preparation method thereof, and belongs to the field of medical products. The method comprises the following steps: preparing a ferroferric oxide-loaded mesoporous silica core-shell structure, loading a chemotherapeutic drug and potassium permanganate, and performing in-situ reduction through ascorbic acid to obtain drug-loaded core-shell nanoparticles; coating the nanoparticles with eudragit S100 to form a pH response layer, and performing amination to prepare an intermediate product; the preparation method comprises the following steps: aminating aldehyde hyaluronic acid through ethanediamine, coupling with MMP-2 sensitive peptide and connecting with folic acid-N-hydroxysuccinimide ester in sequence to prepare folic acid modified enzyme response hyaluronic acid; and finally covalently grafting to the intermediate product to obtain the target composite nano material. The material has pH response enteric protection, MMP-2 enzyme triggered drug release and folic acid-hyaluronic acid dual-targeting functions, is suitable for targeted chemotherapy of gastrointestinal tumors, and can improve the curative effect of drugs and reduce toxic and side effects.