48 results about "Protective enzymes" patented technology

The invention discloses a preparation method of efficient antioxidant sturgeon protein peptide. The preparation method comprises the following steps: S1, extracting a sturgeon skin crude protein solution; s2, extracting sturgeon swimming bladder crude protein; s3, extracting crude protein liquid of the sturgeon meat; s4, performing mixed enzymolysis; and S5, carrying out enzyme deactivation treatment on the obtained enzymatic hydrolysate to obtain enzymatic hydrolysate, and sequentially carrying out ultrafiltration and vacuum drying on the enzymatic hydrolysate to obtain the efficient antioxidant sturgeon protein peptide. Proteins extracted from different parts of sturgeons are mixed according to a specific proportion and then subjected to enzymolysis, the added neutral protease and papain can achieve a synergistic effect, protein is hydrolyzed more comprehensively, small molecule peptides with specific functions are generated, meanwhile, glutathione added during enzymolysis can protect sulfydryl of enzymes, and the enzymolysis effect of the sturgeons is improved. The enzyme activity is maintained, so that the finally obtained sturgeon protein peptide has good oxidation resistance.

The invention provides a novel nano material as well as an immobilized enzyme and application thereof, and relates to the fields of biotechnology and application, material synthesis and application, water environment treatment and environmental protection. The particle size of the nano material is 2-30 microns, and the aperture of the surface is 2-30 nm; the solid nanomaterial is prepared by the following method: adding an alkali solution into a metal salt solution, fully mixing, then adding a ligand aqueous solution, fully reacting, and then separating to obtain the solid nanomaterial. When the material is used for immobilizing enzyme, the particle size of the obtained immobilized enzyme is 2-20 microns, and the aperture of the surface is 1-15 nm; the preparation method comprises the following steps: mixing an enzyme with a ligand aqueous solution, then adding the mixture into a metal salt mixed solution, and separating a solid product. The nano material has excellent biocompatibility and mild synthesis conditions, can protect the reaction activity of the enzyme under harsh conditions such as high temperature, acid and alkali, and improves the recycling capability and storage capability of the enzyme.

The invention discloses a preparation method of a mycotoxin degrading enzyme intelligent slow-release feed additive, and relates to the technical field of feed additive production, a double-emulsion embedding technology is adopted, enzyme is embedded in a multi-layer shell composed of chitosan, sodium alginate and an enteric polymer Eudragit S100, and then Ca < 2 + > ion cross-linking solidification is performed to obtain the mycotoxin degrading enzyme intelligent slow-release feed additive. And finally, freeze-drying or spray-drying to obtain the core-shell structure microcapsule. The obtained microcapsules are compounded with functional carriers such as yeast cell walls, sodium aluminosilicate, mannan oligosaccharide, talcum powder and the like to prepare finished products which can be directly added into various feeds. The release rate of the additive in simulated gastric juice with the pH value of 2.0 for 4 hours is lower than 15%, the release rate of the additive in simulated intestinal juice with the pH value of 6.8 for 6 hours exceeds 90%, the enzyme activity can be effectively protected, and targeted release of intestinal tracts is achieved; meanwhile, the compound carrier has a synergistic adsorption effect on various mycotoxins, and the degradation rates of aflatoxin B1, zearalenone and ochratoxin A in an in-vitro experiment respectively reach 87% or above.

The present invention discloses a preparation method of antioxidant sturgeon protein peptides, comprising the following steps: S1, extraction of crude sturgeon skin protein solution; S2, extraction of crude sturgeon swim bladder protein; S3, extraction of crude sturgeon meat protein solution; S4, mixed enzymatic hydrolysis; S5, subjecting the obtained enzymatic hydrolysis product to enzyme inactivation treatment first to obtain an enzymatic hydrolysis solution, and then sequentially ultrafiltering and vacuum drying the enzymatic hydrolysis solution to obtain antioxidant sturgeon protein peptides. The present invention mixes the proteins extracted from different parts of sturgeon in a specific ratio, and then performs enzymatic hydrolysis. The neutral protease and papain added can act synergistically to more comprehensively hydrolyze proteins, generating small peptides with specific functions. At the same time, glutathione added during enzymatic hydrolysis can protect the sulfhydryl groups of the enzyme and maintain the enzyme activity, so that the finally obtained sturgeon protein peptides have good antioxidant properties.

The invention discloses a high-efficiency green extraction method of semen plantaginis chitin, and belongs to the technical field of natural product extraction. The method comprises the steps of raw material pretreatment, ultrasonic-assisted enzymolysis, enzyme deactivation, centrifugal separation, ultrafiltration concentration, alcohol precipitation and drying. The core innovation lies in that an ultrasonic-assisted composite enzymolysis synergistic technology is adopted, the dissolution rate of the polysaccharide is remarkably increased, the enzyme activity is protected, and finally the high-purity polysaccharide with the molecular weight of 9.2 kDa and the extraction rate of 13.82% is obtained. Compared with a traditional method, intermittent ultrasound and low-temperature enzymolysis are cooperated, so that polysaccharide chain breakage is effectively reduced; the compound enzyme degrades cell wall components in a targeted manner and breaks through the efficiency bottleneck of a single enzyme; toxic solvents are avoided in the whole process, and the process is environment-friendly. The data of the embodiment shows that the extraction rate is increased by 30% or above compared with that of a single enzyme method, the molecular weight distribution of the product is uniform, and the method is suitable for large-scale production of functional polysaccharides in the fields of food, medicine and the like.

The invention relates to the technical field of wastewater treatment, and particularly discloses a degradation method and application of microplastics in aquaculture wastewater. The degradation method comprises the steps of preparation of the mesoporous structure magnetic immobilized material, pretreatment, adsorption-enzymolysis, microbial degradation, magnetic separation, precipitation and the like. The compound enzyme is efficiently fixed through the shell layer of the mesoporous material and the mesoporous structure, enzyme loss is avoided, and the total microplastic removal rate reaches 94.5% or above in cooperation with targeted degradation of the compound enzyme on PET, PE and PP; the composite thermoduric bacteria relay enzymatic hydrolysate is deeply mineralized, and a staged treatment agent is matched to enhance mass transfer, so that the microbial activity is improved by 3 times; the mesoporous material is protected by a shell layer, the enzyme activity retention rate exceeds 85%, the COD of effluent is smaller than or equal to 50 mg / L, the effluent meets the emission requirement, and the method is suitable for multi-scene micro-plastic pollution treatment and has high efficiency, economical efficiency and environment friendliness.

The application relates to the technical field of wastewater treatment, and particularly discloses a degradation method and application of microplastics in aquaculture wastewater. The degradation method comprises the steps of preparation of a mesoporous structure magnetic immobilized material, pretreatment, adsorption-enzymolysis, microbial degradation, magnetic separation, precipitation and the like. The shell layer and the mesoporous structure of the mesoporous material are used to efficiently immobilize composite enzymes, so that enzyme loss is avoided, and the composite enzymes are used for targeted degradation of PET, PE and PP, and the total microplastic removal rate is more than 94.5%. The composite heat-resistant bacteria relay enzymolysis product is deepened and mineralized, and a phased treatment agent is matched to enhance mass transfer, so that the microbial activity is increased by 3 times. The mesoporous material is protected by the shell layer, the enzyme activity retention rate is more than 85%, the effluent COD is less than or equal to 50 mg / L, meets the discharge requirements, is suitable for microplastic pollution treatment in multiple scenes, and has the advantages of high efficiency, economy and environmental friendliness.

The invention belongs to the technical field of agricultural environment protection, discloses an agricultural plant pesticide residue degradation preparation based on laccase, lipase and catalase and a preparation method thereof, and belongs to the technical field of agricultural environment protection. The degradation preparation comprises the following components in percentage by mass: 0.5-3% of laccase, 0.3-2.5% of lipase, 0.2-2% of catalase, 1-5% of a stabilizer, 5-15% of a buffer agent and the balance of water, the stabilizer is one or a combination of more of sodium alginate, chitosan and glycerol, and the buffer agent is one of three buffer solutions of a disodium hydrogen phosphate-sodium dihydrogen phosphate buffer solution. The preparation method comprises the following steps: weighing raw materials, preparing a buffer solution, dissolving a stabilizer, adding enzyme, stirring and mixing, carrying out temperature-controlled reaction, filtering and sterilizing and the like. According to the preparation, through the synergistic effect of the three enzymes, various pesticide residues such as organic phosphorus, organic chlorine and pyrethroids can be efficiently degraded, the stabilizer protects the enzyme activity, the buffering agent maintains the proper pH value, the preparation method is simple and environmentally friendly, and the quality safety of agricultural products can be effectively guaranteed.

The present application relates to a kind of metal-organic framework mediated multi-enzyme nanoparticles and its application, especially the method for alpha-amylase detection. By detecting product absorbance, the quantitative detection of alpha-amylase activity is realized. According to the nature of MFM analog enzyme and its ability as natural enzyme carrier, Agl@MFM@Gox@MFM is prepared, compared with natural enzyme, the composite material has strong resistance, can successfully protect the activity of enzyme. Based on the cascade catalytic system of multi-enzyme nanoparticles Agl@MFM@Gox@MFM, a biosensor is first synthesized and established for detecting alpha-amylase, successfully combines chemical catalyst with natural enzyme, has wide detection linear range (5-500 U / L) while has excellent specificity to alpha-amylase. Benefited from the advantages of low cost, easy preparation and excellent stability, the colorimetric sensor has been successfully used to determine the activity of alpha-amylase in koji.

The invention provides a Tibetan tea insoluble dietary fiber and an extraction method thereof, Tibetan tea powder is subjected to freezing ball milling-low temperature plasma gas phase modification synergistic pretreatment to destroy the compact structure of cell walls and improve the hydrophilicity of the tea powder, then multi-enzyme sequential enzymolysis is carried out under the assistance of an alkyl glycoside-chitosan / graphene quantum dot compound, and the Tibetan tea insoluble dietary fiber is obtained. In the compound, the graphene quantum dots can permeate and expand pores, the alkyl glycoside can expand a fiber structure and protect enzyme activity, and the chitosan can adsorb impurities, so that the inhibition effect of polyphenol on enzyme is effectively overcome, and the enzymolysis efficiency and the impurity removal effect are improved. Through the synergistic effect of physical wall breaking and functional compound auxiliary enzymolysis, the extraction purity and yield of the insoluble dietary fibers of the Tibetan tea are remarkably improved, and a new way is provided for high-value utilization of Tibetan tea resources.

The invention discloses a cage-shaped nanogel immobilized enzyme as well as a preparation method and application thereof. The preparation method comprises the following steps: carrying out Schiff base reaction on transglutaminase and oxidized sodium alginate to prepare an OSA-TG conjugate; carrying out electrostatic self-assembly on the methacrylate / quaternized double modified dextrin and the OSA-TG conjugate, so as to prepare MQD / OSA-TG nanoparticles; the MQD / OSA-TG nano particles are subjected to a free radical polymerization reaction and a calcium ion cross-linking reaction, and MQD / OSA-TG double-cross-linked nano gel is prepared; and degrading the MQD / OSA-TG double-crosslinking nanogel by adopting a chelating agent and a pH (Potential of Hydrogen) regulator, so as to prepare the cage-shaped nanogel immobilized enzyme. The cage-shaped nanogel provided by the invention can protect the protein structure of the enzyme, maintain the enzyme activity and improve the enzyme stability, and meanwhile, the provided method has the advantages of simplicity in operation, safety, reliability and the like, and has huge industrial application potential.

The invention discloses a formula and a preparation process of an environment-friendly paint remover capable of being rapidly stripped at normal temperature, and relates to the technical field of paint removers, and an efficient viscosity reducer comprises the following components: a paint layer decomposing enzyme, a cellulose protecting enzyme, a buffer system, a solvent and a surfactant. According to the invention, high-activity fungal laccase modified by genetic engineering is adopted as a paint layer decomposing enzyme, the enzyme can decompose a paint molecular chain in a targeted manner at normal temperature, the paint removal efficiency is remarkably improved, meanwhile, cellulase capable of forming a protective film on the surface of a base material is cooperatively used, the base material is effectively protected from being damaged, and the combination not only realizes rapid paint removal, but also has the advantages of simple operation and low cost. The pollution to the environment is also reduced, and the green and environment-friendly production requirements are met.

The invention relates to the technical field of agricultural production, and particularly discloses a humic acid biochar composite preparation and a preparation method and application thereof, and the humic acid biochar composite preparation is composed of 120-480 kg / mu of biochar and 49-51 L / mu of 100-400 mg / L of humic acid. The humic acid biochar composite preparation provided by the invention can significantly improve the activity of millet protective enzyme, improve the physical and chemical properties of soil, enhance the enzyme activity of soil, enhance the stress resistance of millet and the fertility of millet planting soil, and promote the growth and development of millet seedlings.

The application provides a high-efficiency detergent and a preparation method thereof, and relates to the technical field of daily chemical products.The detergent comprises microencapsulated composite enzymes, chelating agents, surfactants, additives and water; the active ingredients of the microencapsulated composite enzymes are composed of lipase, protease and amylase, and are prepared by sodium alginate-chitosan microencapsulation embedding, so that the enzyme activity is effectively protected, the stability of the enzymes in the detergent system is improved, and the targeted decomposition of composite stains is realized; the chelating agents are composed of tetrasodium iminodisuccinate and maleic acid-acrylic acid copolymer sodium salt, and can synergistically improve the hard water resistance and the stain removal efficiency; the surfactants are composed of sodium fatty acid methyl ester sulfonate, alkyl polyglycoside and cocamide propyl betaine, and have strong emulsifying and stain removing power and mildness.The detergent is suitable for cleaning tableware and fruits and vegetables, and has the characteristics of high stability, strong stain removing power, easy rinsing, mildness and environmental protection.

The utility model discloses an anti-pollution pesticide residue detection card, which belongs to the field of pesticide residue detection and comprises paper, a crease is arranged in the middle of the paper, a supporting plate is fixedly connected to one side of the paper, an enzyme sheet is arranged on the supporting plate, a first film is arranged at the upper end of the enzyme sheet, the first film is adhered to the top of the supporting plate, and the first film covers the enzyme sheet. The first film is larger than the supporting plate. A groove is formed in the other side of the paper, and a second film is arranged at the upper end of the annular ring, pasted to the annular ring and used for blocking an upper end opening of the annular ring. According to the pesticide residue detection card disclosed by the invention, the first film is adhered to the top of the supporting plate, covers the enzyme sheet and is used for protecting the enzyme sheet from external pollution; the second film is adhered to the annular ring to block the upper port of the annular ring, so that the substrate sheet and a possible reaction area are protected from external pollution, the detection accuracy is improved, the quality guarantee period of the detection card is prolonged, and the use cost is reduced.

The present invention relates to a composition based on ethanolic extract of Brassica olearacea var. capitata leaves, anhydrous lanolin and castor oil, for the treatment of burns and their complications, such as infections. Emulsions made from ethanolic extract of Brassica olearacea var. capitata leaves, anhydrous lanolin and castor oil in semi-solid formulations such as creams and ointments, with 10% and 20% w / v of Brassica extract, can be used as healing agents, accelerating closure of the skin area damaged by up to 3rd degree burns, inducing the production of protective enzymes as well as the proliferation of cells and blood vessels, modulating the production of matrix components and stimulating the formation of strong scar tissue, while having no side effects due to their lack of toxicity.

The present application relates to the technical field of bioengineering, and particularly relates to a beta-glucosidase freeze-drying protective agent and application thereof in production of CK. The present application utilizes beta-glucosidase Bgp3 to prepare rare ginsenoside CK, adopts a freeze-drying technology to prepare a freeze-dried powder from Bgp3 protein supernatant, and adds a specific freeze-drying protective agent before freeze-drying, so as to protect the enzyme activity, improve the enzyme stability, and facilitate storage and longer preservation time. The present application adds a freeze-drying protective agent with a combination of trehalose, arginine and mannitol as raw materials, and the Bgp3 freeze-dried powder prepared without the protective agent has high catalytic efficiency. The present application provides a method for producing rare ginsenoside CK by utilizing beta-glucosidase Bgp3, which has high bioconversion rate, mild reaction conditions, simple equipment and easy operation, and provides technical support for industrial production of rare ginsenoside CK.

The invention provides application of beauveria bassiana HFBb18 in preparation of a green peach aphid biological control entomopathogenic fungus agent, and belongs to the technical field of agricultural biology. The inventor acquires and identifies three new strains of beauveria bassiana from the field, then selects three strains of beauveria bassiana preserved in a laboratory, and finds that the newly found HFBb18 strain has high toxicity to green peach aphids through indoor toxicity determination. On the basis, the influence of infection of the HFBb18 strain on the activities of detoxifying enzyme and protecting enzyme of green peach aphid is determined. Furthermore, the wettable powder taking the HFBb18 strain conidia as a main active ingredient is prepared, the potting pesticide effect test control effect of the wettable powder on green peach aphids is defined, and a foundation is laid for development and application of a novel fungal aphid killing agent.

The utility model relates to the technical field of filtering devices, and particularly discloses a multi-stage fine filtering device for a biological enzyme preparation, which comprises a pre-filter, a coarse filter and a fine filter which are arranged in sequence, a steam filter is arranged between the coarse filter and the fine filter; wherein each of the pre-filter, the coarse filter and the fine filter comprises a base, a cover body arranged at the top of the base, a locking piece for fixing the cover body at the top of the base and a microporous membrane filter element mounted at the top of the base and arranged in the cover body, and an input port communicated with an input cavity in the base is formed in the top of the base; according to the utility model, the series design of pre-filtration, rough filtration, steam sterilization and fine filtration reduces the impurity load step by step, the steam filter is arranged between the rough filtration and the fine filtration, and the sterilized liquid is subjected to fine filtration, so that the filtration precision is improved, the enzyme activity is prevented from being damaged due to incomplete sterilization or high temperature, the sterilization and filtration are synergistic, the sterility is ensured, and the enzyme activity is protected at the same time.

The application provides a kind of cluster root fungus agent for improving the cold resistance of durian and a preparation method thereof, and belongs to the technical field of microbial inoculant.The preparation method of the cluster root fungus agent comprises the following steps: collecting subfrigid zone plant rhizosphere soil, and screening cluster root fungus spores in the soil;inoculating the cluster root fungus spores into sand mixed culture medium, then planting white clover or ryegrass and other plants to carry out enrichment culture and obtain culture;mixing the culture with cutting substrate, and cutting one-year-old durian branches to carry out rooting culture and first low-temperature culture;storing the root system of cutting seedlings in cold storage, crushing, mixing with cutting substrate, cutting one-year-old durian branches to carry out second low-temperature culture, and collecting the root system of cutting seedlings and rhizosphere soil to obtain the cluster root fungus agent.The cluster root fungus agent can induce durian to produce low-temperature resistant protective enzymes, thereby improving the cold resistance of durian and providing a new idea for cultivating durian cold-resistant varieties.

The invention provides a PCR (Polymerase Chain Reaction) premixed solution with high sensitivity and rapid amplification, a PCR amplification kit and a PCR method, and relates to the technical field of biology. The PCR premixed solution provided by the invention comprises a PCR enhancer with specific composition, glycerol and TMAC (tetramethylammonium chloride) can reduce non-specific amplification of primers, and the combined use effect of the glycerol and the TMAC is superior to that of any single component; glycine betaine and gelatin have the effect of enhancing sensitivity, and the combined use effect of glycine betaine and gelatin is better; glycerol and betaine can increase the duration of tolerating rapid heating and cooling, have a superposition effect, and do not have an antagonistic effect with other components at the same time; the glycerol and the betaine can obviously improve the stability of the Taq enzyme and obviously increase the temperature tolerance. Therefore, the enhancer provided by the invention can protect the activity of enzyme, promote the amplification efficiency and ensure the stability and accuracy under the condition of rapid amplification.

The application discloses a DREB transcription factor CtDREB1B of safflower and application thereof in positive regulation of drought and salt stress resistance of plants. The full-length nucleotide sequence of the gene CtDREB1B is shown as SEQ ID NO. 2, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 1. The application first clones the complete coding frame of the gene by RT-PCR, clones the gene into a pYES2 vector and transfers the gene into yeast, and finds that the pYES2::CtDREB1B yeast cells can still grow on SGR culture medium containing 1.5 M mannitol or 4 M NaCl. Then, the CtDREB1B gene is heterologously expressed into Arabidopsis thaliana. Overexpression of the CtDREB1B in the Arabidopsis thaliana can make the transgenic Arabidopsis thaliana exhibit a drought and salt tolerance phenotype, and the activities of protective enzymes such as SOD and POD are enhanced, and the expression amounts of stress resistance related genes such as AtABF3 and AtRD29A are up-regulated, indicating that the gene can simultaneously positively regulate drought and salt stress. Therefore, the gene can be applied to breeding of new plant stress resistance varieties, and in addition, the gene provides a new gene resource for plant stress resistance, and has important application prospects in breeding of salt-tolerant plants.

Antimicrobial preservative compositions that can be incorporated in products to be preserved are disclosed herein. The disclosed compositions include immobilized biocidal enzymes, such as cross-linking enzymes or active enzymes falling into the zymogen-class, for the purpose of modifying amino acid residues on a protein or binding a molecule of interest to a protein. The compositions include enzymes that are immobilized on a polymeric solid support, which improve the shelf life of the enzyme and protect the enzyme from auto-cross-linking or other deterioration over extended storage periods. Also disclosed are methods of increasing the self-like of products, such as personal care, household and industrial products, by incorporating an effective amount of the disclosed compositions into the product.

The present disclosure relates to protection and / or enhancement of enzymatic activity. The invention provides an application of a fullerene derivative in protecting and / or improving enzyme activity, the fullerene derivative has a modification group for increasing water solubility, and an enzyme protective agent comprising the fullerene derivative. The fullerene derivative material disclosed by the invention is stable in structure, good in water solubility and excellent in biocompatibility, and can effectively remove damage factors around an enzyme under the condition which is possibly encountered in industrial production and enzyme catalysis engineering and is not beneficial to enzyme catalysis activity, so that the efficiency of a catalytic substrate is improved, and the yield of the product is improved. A novel efficient enzyme protection material with excellent biocompatibility is constructed. The fullerene derivative material disclosed by the invention is non-toxic and non-dangerous, can be carried out under mild reaction conditions, is simple and controllable, is high in practicability, and effectively widens the variety range of protective enzymes.

The invention discloses a method for rapidly detecting sulfonamides in meat by an improved QuEChERS combined enzyme inhibition colorimetric method, which uses a natural eutectic solvent instead of a toxic organic solvent as an extraction agent, reduces the harm to the environment and human body, improves the extraction efficiency, and is beneficial to protecting the enzyme activity, so that the detection is more sensitive and stable; the magnetic COF (TpPa-Cl) coated Fe3O4 synthesized by adopting a simple and rapid stirring method not only has good chemical stability and can be repeatedly used, but also has excellent adsorption capacity and strong magnetism, and when the magnetic COF (TpPa-Cl) coated Fe3O4 is used as an adsorbent for improving QuEChERS, interfering substances in a sample can be effectively removed in a small amount, the pretreatment time is shortened, and the purification process is simplified; in the enzyme inhibition colorimetric detection process, a digital image colorimetric method of a smart phone is combined for quantitative analysis. The whole method has the remarkable advantages of greenness, high efficiency, short time consumption, low cost, simplicity in operation, high sensitivity and the like.

The present invention discloses a method for preparing a diglyceride product by a solvent-free enzymatic method, and belongs to the field of oils and fats. The present invention pre-adsorbs glycerol by a cellulose adsorbent, combines a fixed bed reaction column to fix the adsorption material, and continuously feeds a glycerol oil mixture of a suitable concentration into a reaction system. At the same time, polar resin and lipase are added to the reactor, and the ability of the polar resin material to adsorb excess polar substrates in the system is utilized to protect the enzyme active center, effectively extend the service life of the lipase, and significantly reduce the cost of preparing diglyceride by enzymatic glycerolysis reaction. At the same time, the present invention achieves the purpose of reducing the reaction time and reducing the amount of enzyme added while ensuring a high diglyceride yield of the product by screening the adsorbent and controlling the water content of the reaction system. The diglyceride of the crude product prepared by the method of the present invention is >50wt%, and the diglyceride after molecular distillation is >85wt%, which has a certain industrial application prospect.

The invention discloses application of cinnamomum longepaniculatum essential oil in prevention and control of stored grain pests, and relates to the technical field of stored grain pest prevention and control. According to the method disclosed by the invention, the fumigation, contact toxicity and avoidance effects of the cinnamomum longepaniculatum crude oil, the cinnamomum longepaniculatum residual oil light components obtained after extraction and processing of the cinnamomum longepaniculatum crude oil, alpha-terpilenol and 1, 8-cineole on rice weevil and tribolium castaneum adults are analyzed by utilizing indoor toxicity determination; changes of the essential oil alpha-terpilenol with the best effect on activity of protective enzyme, detoxification enzyme and digestive enzyme in the two stored grain pests are determined, and a reference basis is provided for preventing and controlling rice weevil and tribolium cavaleriei by scientifically and reasonably using the cinnamomum longepaniculatum essential oil. The alpha-terpilenol can be used as a potential biopesticide for rice weevil and tribolium cavaleriei, and lays a theoretical foundation for molecular research and green prevention and control of stored grain pests. The invention effectively solves the problems of pest resistance, public health and safety and environmental pollution easily caused by a chemical control strategy.

The invention provides a process for preparing L-2-aminobutyric acid by an immobilized enzyme method. The preparation method comprises the following steps: firstly, enabling graphene oxide, hexadecyl trimethyl ammonium bromide and tetraethoxysilane to react, growing fibrous mesoporous silica in situ, forming a compound with a mesoporous structure and a through large-aperture channel, providing binding sites for enzyme and promoting diffusion of a substrate / product; secondly, through ternary polymerization of a double-bond-containing graphene oxide-silicon dioxide compound, double-bond-containing chitosan and double-bond-containing cyclodextrin, a stable carrier network is constructed, and the enzyme immobilization efficiency is enhanced by virtue of the biocompatibility of chitosan and cyclodextrin and active groups; and finally, embedding the carrier and the enzyme by using sodium alginate-calcium chloride gel, protecting the enzyme activity conformation and constructing a stable microenvironment. The method solves the problems that the existing enzyme is poor in stability and cannot be reused, maintains high catalytic activity and enantiomer purity while reducing the enzyme cost, and has industrial application prospects.

The invention belongs to the technical field of detection, and particularly relates to a composite electrode, a preparation method and application thereof, and a detection method of organophosphorus pesticide content. The composite electrode provided by the invention adopts the oxide with a three-dimensional ordered macroporous structure as a supporting platform, has a relatively large specific surface area and good conductivity, and effectively improves the immobilization efficiency and stability of a biological material. By loading high-dispersion noble metal, the electro-catalytic performance is further enhanced, and the sensitivity of electrochemical reaction is improved. Acetylcholinesterase (AChE) is used as a biological recognition element, and efficient detection of pesticide residues is achieved by means of the inhibition effect of AChE on organophosphorus pesticides. Due to the introduction of noble metal, the catalytic efficiency of electrochemical reaction is enhanced, and the sensitivity of the sensor is improved. Meanwhile, the three-dimensional ordered macroporous structure can effectively protect the activity of enzyme molecules and avoid inactivation of the enzyme molecules in the detection process, so that the stability and repeatability of the sensor are improved.

The invention discloses a high-activity immobilized plastic degrading enzyme preparation and application thereof, and belongs to the technical field of plastic degradation. The enzyme preparation is prepared by the following steps: by taking pinus tabulaeformis pollen as a raw material, performing acid treatment, carbonization and activation to form a hierarchical porous active pollen carrier; through lanthanum-doped magnetic nanoparticle modification and sulfydryl functional modification, the carrier is endowed with magnetic responsiveness and directional fixed sites; a three-dimensional cross-linked structure is constructed by using a triazine ring rigid conjugated monomer and a hyperbranched network, and spatial confinement immobilization of enzyme molecules is realized by combining an ultraviolet click reaction; and cutinase, lipase, cellulase, laccase and peroxidase are synergistically loaded to form a multi-enzyme catalytic system. By combining pollen-based hierarchical pore channels with a triazine ring rigid network, the enzyme loading capacity and distribution uniformity are remarkably improved, the enzyme activity retention rate of 90% or above is still kept after an enzyme preparation is recycled for 10 times through cross-linked network protection, and rapid magnetic separation is achieved through lanthanum-doped ferroferric oxide nanoparticles.