106results about How to "Maintain enzyme activity" patented technology

A composition of enzyme, polymer, and crosslinker forms a network of covalently bound macromolecules. The covalently immobilized enzyme preparation has enzymatic activity, and retains stable activity when dried and stored at ambient conditions. Methods for preparing an immobilized enzyme and methods for using the enzyme are disclosed.

A composition for hydrogels which comprises (A1) a copolymer which comprises 25 to 97 mol % of unit (a) derived from a compound represented by formula (1) and 3 to 75 mol % of unit (b) derived from a monomer represented by formula (2) and is soluble in aqueous media and (B) a polymer which has hydrophilic groups such as hydroxyl or carboxyl groups and is soluble in aqueous media; and a hydrogel comprising the composition. (X represents a divalent organic residue; Y represents alkyleneoxy, etc.; Z, R1, R2, R3, R4, and L2 each represents hydrogen, etc.; and L1 represents —C6H4—, etc.)

The invention belongs to the enzyme immobilization field and in particular relates to a method for preparing immobilized laccase in electrospun fiber membrane by utilizing emulsion electrospinning technology. The method is characterized by in-situ embedding and immobilizing the laccase in the electrospun fiber by the emulsion electrospinning technology. The carrier material of the immobilized laccase is polylactic acid-caprolactone copolymer (P(LA / CL), 7 / 3, namely the ratio of polylactic acid to polycaprolactone during synthesis) with good biocompatibility. The method specifically comprises two steps of preparing the enzyme-carrying electrospun fiber membrane and immobilizing the enzyme-carrying electrospun fiber membrane by crosslinking. The first step is as follows: first preparing polymer solution and laccase solution into homogeneous emulsion, then carrying out electrospinning on the emulsion and obtaining the enzyme-carrying electrospun fiber membrane a few hours later. The secondstep is as follows: exposing the membrane in glutaradehyde saturated vapor to be crosslinked, thus obtaining the immobilized laccase in the electrospun fiber membrane. The invention provides the new,simple and efficient method preparing the immobilized laccase, and the immobilized laccase obtained by the method has high catalytic activity and good stability and features low preparation cost.

The present invention relates to a process for preparing a polymer / biological entities alloy, comprising a step of mixing a polymer and biological entities that degrade it, during a heat treatment, said heat treatment being performed at a temperature T above room temperature and said biological entities being resistant to said temperature T, characterized in that said biological entities are chosen from enzymes that degrade said polymer and microorganisms that degrade said polymer.

The invention relates to an SOD complex capsule and a preparation method thereof. The SOD complex capsule contains resveratrol, coenzyme Q10, goose liver extracts, wheat germ extracts, safflower extracts and tartary buckwheat extracts; the weight ratio of the components is 10-50 : 10-75 : 75-150 : 75-150 : 75-150 : 200-500; every 100g of the complex capsule contains 40000-4000000 activity unit of SOD which is a tadpole extract. The preparation method is as follows: processing grape skins, pig hearts, goose livers, wheat, safflower, tartary buckwheat and tadpole by the steps of breaking, concentration, centrifugal separation, purification and the like to obtain active ingredients of various raw materials, embedding the tadpole extract for several times, mixing the tadpole extract with other components and palletizing the mixture, thus obtaining the finished product. The invention has the function of oxidation resistance, can alleviate physical fatigue and strengthen immunity of human body.

The invention discloses a culture medium capable of promoting the division growth of mesenchymal stem cells, which comprises a serum-free basic culture medium and an additive added on the basis of theserum-free basic culture medium, wherein the additive comprises a hibiscus mutabilis extract, seaweed polysaccharide, astragaloside IV, human serum albumin, transferrin, glutamine, platelet-derived factor, epidermal growth factor, fibroblast growth factor, human insulin growth factor and vitamin A; the hibiscus mutabilis extract has antioxidant and cell activating activity, and can effectively promote the growth of cell metabolic by compounding seaweed polysaccharide and astragaloside IV; human serum albumin, transferrin, glutamine and vitamin A provide essential nutrient substances for the growth of stem cells; platelet-derived factor, epidermal growth factor, fibroblast growth factor, human insulin growth factor and other cytokines together promote the rapid growth and proliferation ofstem cells; the culture medium not only can improve the growth activity of mesenchymal stem cells, shorten the culture time, promote the expression of cell growth factors, but also can maintain the stem cell activity of the differentiation potential of mesenchymal stem cells; stem cells are not differentiated in daily culture, thereby providing convenience for scientific research.

The invention belongs to the technical field of materials and discloses a soybean epoxy compound hydrolase immobilization method and an enzyme preparation. The soybean epoxy compound hydrolase immobilization method comprises the following steps: dispersing soybean epoxy compound hydrolase in a buffer solution with the pH of 5-9 to prepare an enzyme solution, mixing the enzyme solution with UiO-66-NH2 nano crystals to obtain a mixture, adding a saturate ammonium sulfate solution in the mixture at 0-35 DEG C to enable the final saturability of ammonium sulfate to be 80%, stirring and mixing for 1-60 min to obtain a mixed turbid liquid, mixing the mixed turbid liquid with a glutaraldehyde solution, reacting for 1-200 min in a stirring manner, and freeze-drying to obtain a soybean epoxy compound hydrolase preparation. According to the invention, a simple sedimentation crosslinking technology is adopted, the cost is lower, the reaction is moderate, the enzyme activity can be kept to a greater degree, the enzyme loading amount of a carrier can be increased, the enzyme activity recovery rate can be increased, and the immobilization effect is excellent.

The invention discloses a surgical instrument two-cavity multienzyme coagulated bead. The multienzyme coagulated bead uses a water-soluble macromolecular film as a coating material for forming the coagulated bead with two cavities; the two cavities comprise a cavity 1 and a cavity 2; solid enzymes are put into the cavity 1; super concentrated cleaning agents are put in the cavity 2, and include nonionic surfactants, ampholytic surfactants, corrosion inhibitors, chelating agents, sterilizing agents and solvents; the solid enzymes consist of protease, lipase, amylase and cellulase. The surgicalinstrument two-cavity multienzyme coagulated bead has the advantages that the cleaning capability is high; the enzyme activity stability is high; safety and toxicity are realized; the surgical instrument two-cavity multienzyme coagulated bead is applicable to medical appliance cleaning.

A method for degrading corn straws is characterized by comprising the following steps: specifically mechanically crushing, sieving and drying the corn straws, then degrading lignin in the corn strawsby using immobilized laccase taking Cu2+ modified Fe3O4-NH2-PEI magnetic nanoparticles as carriers, recovering the immobilized laccase, boiling the degraded corn straws, then adding NaOH for washing,and adding cellulase for further degrading the cellulose. According to the method, the affinity between the immobilized laccase and a substrate is enhanced, the stability and the activity of the laccase in an application process are improved, thus, the degradation efficiency is improved, and the degradation rate of the lignin reaches 40.76% or above. Inhibition of the immobilized laccase and degradation products such as phenolic substances generated by degradation on the cellulase activity is eliminated, so that the cellulase activity is guaranteed, and the conversion rate of the cellulose isimproved, and reaches 46.85%.

The present invention discloses a yeast engineering strain which can produce the human pepsinogen, the yeast strain is microzyme which is converted through a eukaryotic expression vector, and the eukaryotic expression vector contains the protogene of the human pepsinogen. The present invention also discloses the preparation method and the application of the yeast engineering strain. The yeast engineering strain of the present invention realizes the highly effective secretion expression of the human pepsinogen zymogen C in a methylotrophic yeast pichia pastoris, the expression quantity is above 600 mg / L, the recombination PGC of the present invention is consistent to the immunogenicity and the proteinase activity of the PGC abstracted from human gorge tissue, thus the present invention can be widely applied to a plurality of fields such as medicine, food and chemical engineering, etc., and have extremely good application value and market prospect.

The invention discloses health-care wine and preparation method thereof. The raw materials of the health-care wine comprise snake venom defibrase mixed liquor, earthworm fibrinolytic enzyme mixed liquor and white spirit, and in addition, traditional Chinese medicines such as pseudo-ginseng, red flower, licorice root, medicinal cyathula root, acanthopanax, and the like can also be added. The health-care wine uses double enzymes, can lighten the toxicity and improve the effect on preventing and curing cardiovascular diseases and has a good anti-thrombus effect. By matching with the traditional Chinese medicines, the health-care wine can activate blood, dissolve stasis, detoxify and stop bleeding and has lower production cost, simple and convenient preparation method and easy popularization.

The invention provides a liquid detergent composition suitable for automatic feeding of a dish-washing machine. The liquid detergent composition comprises a nonionic surfactant, an alkaline auxiliaryagent, a chelating agent, an enzyme preparation, a rheology modifier and deionized water, and the rheology modifier comprises at least one of carboxymethyl cellulose, hydroxyethyl cellulose, xanthan gum, carbomer, sodium chloride and magnesium chloride. The invention provides the liquid detergent composition. The rheology modifier is added to endow the liquid detergent with proper viscosity, so that the liquid detergent is not gelled, layered and separated out after being stored in the cavity of an automatic feeding distributor of the dish-washing machine for a long time, and the liquid detergent composition has stable physicochemical properties, stable viscosity during temperature change and stable enzyme activity, so that the decontamination capability of the liquid detergent is better.

The invention discloses a pungent and spicy barbecue sauce. The pungent and spicy barbecue sauce is not added with any spice essence and preservative, is heavy in barbecue flavor, obvious in main flavor, long in quality guarantee period and strong in tenderizing effect. Especially, due to the scientific compounding of the barbecue sauce, the taste, flavor and functional characteristics (bacterial inhibition, oxidation resistance and pharmaclogical property) of the barbecue food are improved, and comprehensive flavor substances of food materials in a barbecue state are collected, so that the barbecue sauce has heavy and attractive distinctive barbecue flavor, and the problem that food materials have no barbecue flavor before roasting is solved; meanwhile, by virtue of scientific compounding of a base sauce and compound enzyme, the cholesterol contents of food materials and auxiliary materials are reduced, and a new functional substance cholest-4olefine-3ketone is generated, so that the possibility that cholesterol generates carcinogens during high-temperature roasting or combustion is completely eradicated, the food safety is improved, the prepared barbecue food is fresh and tender in texture, fresh and delicious in taste, heavy in barbecue flavor, low in cholesterol content and strong in food safety, and therefore, a new shortcut for preparing barbecue foodstuff with distinctive barbecue flavor is explored.

A combined multi-enzyme capsule is composed of the shell and the core consisting of one or two anti-inflammatory enzymes and auxiliary.

The present invention relates, in general, to autotaxin. In particular, the present invention relates to a DNA segment encoding autotaxin; recombinant DNA molecules containing the DNA segment; cells containing the recombinant DNA molecule; a method of producing autotaxin; antibodies to autotaxin; and identification of functional domains in autotaxin.

The invention discloses an immobilization device and an immobilization method for producing immobilized enzymes or cells. The device is composed of four main parts: a mixer, a material distributor, a titrator and a curing box. , Immobilized carrier and microbial cell (or enzyme) mixed solution preparation, immobilized enzyme titration and curing functions in one, mass production of immobilized enzymes.

The present invention relates to a novel tobacco fermenting enzyme preparation production method. The purpose is to solve the technical problems that how improve the quality of the tobacco fermenting product is improved and how the reactivity protection of the tobacco fermenting enzyme preparation is realized in the natural fermenting field of tobacco. The enzyme preparation consists of a glucoseoxidase, a chlorophyl oxidase, a carotenoid oxidase, a protease, and a nicotine-degradation enzyme. Through the cell disruption of fresh leaves, (NH 4) 2 SO 4 is utilized to operate the second fractional precipitation to obtain crude enzyme fluid, an enzyme molecule adopts Ca 2 + and Mg 2 + to operate the metal ion exchange, to accomplish the molecule modification; a macro molecule combination modification is accomplished through adopting 0.01 percent of cane sugar low molecular polymer, thereby prolonging the half life period of theenzyme preparation and obviously improving the high temperature resistant ability. The experimental result employed by the enzyme preparation indicates that the nicotine is decreased by 9.3 percent, the total nitrogen is decreased by 5.7 percent, the protein is decreased by 7.1 percent; cigarette smoke condensates are decreased by 8.4 percent, the tar content is decreased by 5.1 percent, the cigarette smoke nicotine content is decreased by 28.0 percent, and the carbon monoxide is decreased by 1.6 percent. The enzyme preparation is employed when the tobacco leaf is wet for the second time before defolat and redrying.

The invention discloses a method for preparing different tones of indigo pigments based on blueberry leaves. The method comprises the steps that blueberry leaves are used as raw materials, and different tones of indigo pigments are prepared by extracting and purifying pigments in the blueberry leaves and through reaction with multiple amino acids under the effect of glucosaccharase. The preparation method comprises the following steps of raw material drying and powdering, preparation of an enzyme buffer solution, water extraction pretreatment, filtration, blueberry leaf pigment crude extract purification, drying and concentration, enzymolysis for blue turning and finished product obtaining. By adopting the obtained natural indigo pigments, the product quality is stable, the safety is good,and the product has a better nutrition value. According to the types and use amounts of the added amino acids, different tones of indigo pigments can be prepared.

The invention discloses a special barbecue sauce for pan-fried beef steak with black peppers. The special barbecue sauce is free from any spice, essence and preservatives, rich in roasting flavor, prominent in main flavor (beef flavor), and high in tenderization effect. The mouth feel, the flavor and the functional properties (antibacterial properties, inoxidizability and pharmacology properties)of barbecue foods are improved. Comprehensive flavor substances of food materials (beef) under the barbecue condition are collected, so that the barbecue sauce has rich and attractive special barbecueflavor. The barbecue time is shortened, the generation of carcinogen is reduced, the cholesterol level of food materials (beef) and auxiliary materials is reduced, and a new functional substance namely 3-oxo-4-cholestene is generated. The possibility that cholesterol is barbecued at high temperature or burnt to generate the carcinogen is prevented from the source, and the safety of the barbecue foods is further improved. The beef steak which is made through the barbecue sauce is fresh and tender in meat quality, fresh and delicious in flavor, rich in baking flavor, low in cholesterol level and high in food safety. A new shortcut for preparing the pan-fried beef steak with black peppers with special barbecue flavor is developed.

The invention discloses a method for synthesizing sucrose-6-phosphate from recombinant high-temperature-resistant sucrose phosphate synthase and belongs to the field of enzyme engineering. The methodcomprises the following steps: cloning a gene of a high-temperature-resistant sucrose phosphate synthase, constructing overexpression plasmids, transforming the overexpression plasmids into Escherichia coli BL21 (DE3), inducing expression of the high-temperature-resistant sucrose phosphate synthase, and performing affinity chromatography purification to obtain a recombinant high-temperature-resistant sucrose phosphate synthase, making the recombinant high-temperature-resistant sucrose phosphate synthase with the concentration of 0.01-10 [mu]g / ml, fructose-6-phosphate with the concentration of0.1-20 mM and uridine diphosphate glucose with the concentration of 0.1-20 mM react at 70 DEG C for 1-24h to obtain sucrose-6-phosphate. The optimal reaction temperature of the recombinant high-temperature-resistant sucrose phosphate synthase is 70 DEG C, the tolerable temperature reaches 100 DEG C, the enzyme activity can be kept at 100 DEG C, generation of sucrose-6-phosphate is catalyzed, and the recombinant high-temperature-resistant sucrose phosphate synthase is suitable for industrial production of sucrose-6-phosphate.

The invention discloses a PCR rapid detection method for marine culturable bacteria, and a kit, belonging to the technical field of microorganism detection. The kit comprises a DNA extraction reagent,a PCR reaction reagent and an electrophoretic reagent. Among them, DNA extraction reagent is Chelex- 100; The PCR reaction reagents included two universal 16S rRNA primers (27F and 1492R) and 2 x TaqPCR Mastermix solution. Electrophoretic reagents included agarose, 50 x TBE, Goldview II nucleic acid dye, DNA Marker. The kit can be used for DNA amplification of marine culturable bacteria in a short time, and has the advantages of simple procedure, safety, economy, high efficiency and small bacterial requirement.

The invention discloses a biological enzyme dye accelerator for wool fabrics. The biological enzyme dye accelerator comprises, by weight, 100 parts of complex enzymes, 20-30 parts of stabilizers, 15-19 parts of penetrating agents, 10-15 parts of lanthanum chloride, 9-11 parts of chitosan, 2-5 parts of pentane alcohol sodium phosphate ester, 6-9 parts of dodecylphenol ether and 40-60 parts of water. The invention further discloses a method for applying the biological enzyme dye accelerator for the wool fabrics. The biological enzyme dye accelerator and the method have the advantages that the biological enzyme dye accelerator is excellent in stability, and the excellent activity of the biological enzyme dye accelerator still can be kept even after the biological enzyme dye accelerator is stored for a long time; the wool fabrics can be dyed under low-temperature conditions after being finished by the aid of the biological enzyme dye accelerator, and are excellent in dye-uptake.

The present invention is one kind of food, especially the papaya proteinase throat-moistening tablet or papaya essence throat-moistening tablet is composition of papaya proteinase or papaya fruit milk, beta-cyclodextrin, mannite, proteoglycan, menthol, menthol oil, Arabic gum, ascorbic acid, anhydrous ethanol and water and has a water content below 3%. It is antibacteria, diminish eliminating, can protect throat, has no side effect and can be eaten by all people.

The invention provides carrier-free immobilized rhizopus oryzae lipase as well as a preparation method thereof and an application thereof. The preparation method comprises the following steps: (1) charging a protein cross-linking additive into a buffer solution containing rhizopus oryzae lipase to form a first solution, wherein the protein cross-linking additive is serum albumin; (2) charging glycol dimethyl ether into the first solution to precipitate the rhizopus oryzae lipase, and standing the solution to obtain a second solution containing the rhizopus oryzae lipase; (3) charging a cross-linking agent into the second solution to form a mixed solution, then incubating the mixed solution, wherein the cross-linking agent is selected from acetic anhydride, formaldehyde and glutaraldehyde; (4) precipitating the mixed solution incubated in the step (3), to obtain a cross-linked enzyme aggregation. The carrier-free immobilized rhizopus oryzae lipase is high in catalytic oil transesterification reaction and is still high in activity after being reused for multiple times.

The invention discloses a bio-enzyme preparation preserving method, which belongs to the technical field of biological engineering. The method preserves immobilized beta-galactosidase by washing and sterilizing immobilized beta-galactosidase and storing at lower temperature. For the immobilized beta-galactosidase which is used for decomposing lactose in milk, the method can enable the immobilizedenzyme to be repeatedly used for a long time, keep production continuity and lower production cost. Under a noncontinuous use condition, the adoption of the method can be effectively avoid microbial pollution and effectively retain the activity of the immobilized enzyme. When the method is used, the problems of low activity of the immobilized enzyme, influences on reaction speed and production efficiency and high production cost due to pollution of microbe to the immobilized beta-galactosidase in a use process and influences of proteins, fat and minerals are solved, and the reliable, effective and convenient immobilized enzyme washing, sterilizing and storing of lactose-decomposed milk and galactooligosaccharide-containing products can be realized.

The present invention provides the preparation process of garlic SOD and functional SOD food and beverage, and the prepared garlic SOD products have the SOD enzyme activity maintained, no bad garlic smell and prolonged garlic SOD preserving period. The functional garlic SOD food and beverage has functions of eliminating free radical in body, delay senility, etc. as well as wide-spectrum bactericidal function. Taking the food and beverage regularly can raise body's immunity, raise body's disease resistance, strengthen body's adaptability to outer environment, repair radiotherapeutically and chemotherapeutically damaged cells, and beautify.

Formulations of β-glucuronidase enzymes that exhibit long-term stability at both high and low temperatures as either aqueous or lyophilized formulations are provided. The formulations of the invention comprise a β-glucuronidase enzyme, such as a mutant β-glucuronidase enzyme, an amphoteric compound, L-histidine,β-alanine and a sugar. Methods of preparing the formulations, as well as methods of using the formulations for hydrolysis of glucuronide substrates, including opiates and benzodiazepines, are also provided.