31 results about "Cholestene" patented technology

The invention relates to a method for increasing the yield of 7-dehydrocholesterol in saccharomycetes by using compartmentalization. The method comprises the following steps of: by taking saccharomycetes as an original strain, expressing heterologous sterol delta 24-reductase and delta-cholestenase, wherein the yield of 7-DHC in saccharomyces cerevisiae S288C is detected to be 10.15mg / L. According to the method disclosed by the invention, partial enzymes in a 7-DHC synthesis path are positioned in each compartmentalization in the saccharomyces cerevisiae by using an oxidase and a mitochondrial positioning tag, and a relatively independent 7-DHC synthesis path is formed. Meanwhile, the storage space of precursor substances needed by 7-DHC synthesis is increased, the feedback effect is reduced, the conversion efficiency between enzymes is improved in the same compartment, the loss of the acting substrate is reduced, and finally the yield of the 7-DHC is improved by 4 times and reaches 53.31mg / L.

The present invention discloses one kind of liquid crystal compound in dimesoorphic structure and its preparing process, and the liquid crystal compound is synthesized with corresponding trans-alkylcyclohexyl phenol and cholesterol bromate through reaction in the presence of alkali through heating and stirring inside proper solvent, and contains both cyclohexyl phenol mesoorphic unit ad cholestene base mesoorphic unit. The general expression of the liquid crystal compound is shown. The liquid crystal compound has low and wide-range phase change temperature, simple cholestene phase and high physical and chemical stability, and may be used as liquid crystal material or liquid crystal material component.

The invention discloses a special barbecue sauce for pan-fried beef steak with black peppers. The special barbecue sauce is free from any spice, essence and preservatives, rich in roasting flavor, prominent in main flavor (beef flavor), and high in tenderization effect. The mouth feel, the flavor and the functional properties (antibacterial properties, inoxidizability and pharmacology properties)of barbecue foods are improved. Comprehensive flavor substances of food materials (beef) under the barbecue condition are collected, so that the barbecue sauce has rich and attractive special barbecueflavor. The barbecue time is shortened, the generation of carcinogen is reduced, the cholesterol level of food materials (beef) and auxiliary materials is reduced, and a new functional substance namely 3-oxo-4-cholestene is generated. The possibility that cholesterol is barbecued at high temperature or burnt to generate the carcinogen is prevented from the source, and the safety of the barbecue foods is further improved. The beef steak which is made through the barbecue sauce is fresh and tender in meat quality, fresh and delicious in flavor, rich in baking flavor, low in cholesterol level and high in food safety. A new shortcut for preparing the pan-fried beef steak with black peppers with special barbecue flavor is developed.

The invention relates to a temperature-sensitive color-changing APET material and application thereof. A temperature-sensitive liquid crystal material is microencapsulated through polylactic acid and then is blended with APET for injection molding to obtain corresponding particles. Polylactic acid with a certain molecular weight and temperature-sensitive olefine acid cholesteric liquid crystal are mixed to prepare microcapsules through a phase separation method, and then the microcapsules and APET are blended and subjected to injection molding to obtain particles containing the temperature-change microcapsules. The polylactic acid is a material with the molecular weight larger than 50,000, the temperature-sensitive olefine acid cholesteric liquid crystal comprises cholesteric pelargonic acid ester CPE, cholesteric chloride CC and cholesteric oleic acid carbonic ester COC mixed compound liquid crystal, the compound ratio is 40-70%: 2-10%: 10-30%, and the effects of displaying red at about 15-20 DEG C, developing green at about 23-28 DEG C and developing blue at about 33-39 DEG C in the temperature developing range can be achieved. The color developing temperature error does not exceed 3 DEG C. And after mixing and injection molding with APET, various materials in the forms of films, plates and the like can be prepared. The temperature-sensitive color-changing APET material can be widely applied to the fields of high-end cosmetics, medical and health products, toys for children and the like to obtain corresponding color effects.

The invention discloses a cholesterol oxidase and application thereof, and belongs to the technical field of enzyme engineering. A genetically engineered bacterium E.coli BL21(DE3)-pET20b(+)-COD of the cholesterol oxidase is firstly constructed and obtained, the enzyme activity of the cholesterol oxidase in a crude enzyme solution obtained through fermentation reaches up to 700 U.L-1, the specificenzyme activity of the purified cholesterol oxidase is 18.5 U.mg-1, the molecular weight of the cholesterol oxidase is about 60,000 kDa, and the cholesterol oxidase has good temperature tolerance andorganic solvent tolerance. An engineering bacterium fermentation solution is utilized for preparing cholest-4-alkene-3-ketone and cholest-4-alkene-3,6-diketone, in a two-phase transformation system taking methylbenzene as an organic phase, the transformation rate of preparing the cholest-4-alkene-3-ketone by taking cholesterol as a substrate is 30%, and the transformation rate of preparing the cholest-4-alkene-3,6-diketone is 45%.

The invention discloses a mild and efficient whitening composition which is prepared from the following raw materials in parts by weight: 50-80 parts of a water-soluble component, 5-10 parts of a whitening agent, 7.5-25 parts of an oil-soluble component and 10-30 parts of water. Through synergistic compounding of phospholipid, hydrogenated phosphatidylcholine and 4-butylresorcinol, the problems that 4-butylresorcinol is difficult to dissolve and easy to oxidize and discolor are solved, effective components are more easily absorbed by skin, the whitening and spot-fading effects are improved, and through adoption of cholest-5-ene-3 beta-alcohol, the whitening and spot-fading effects are improved. Through combination and compounding of 4-butylresorcinol, alpha-tocopherol and sunflower seed oil, irritation of 4-butylresorcinol to skin is reduced, the tenderness of a final product is greatly improved, meanwhile, through compounding of phospholipid, hydrogenated phosphatidylcholine and cholest-5-ene-3 beta-alcohol, the lipidosome has good stability, does not contain a surfactant, and is free of toxic and side effects. The use skin feeling of the product is greatly improved, and the skin is fine and smooth and not tight.

The invention provides the use of 5-cholestene-3β-ol sulfate in the preparation of drugs for reducing the apoptosis of pancreatic beta cells, promoting the proliferation of pancreatic beta cells, and protecting the morphology and structure of pancreatic islets, and the preparation of drugs containing 5-cholestene-3β-ol Alcohol Sulfate Drugs. 5-Cholesterene-3β-ol sulfate can reduce the apoptosis of islet β cells, promote the proliferation of islet β cells, protect the morphology and structure of islets, prevent the loss of islet β cells, enhance insulin secretion, and significantly improve the symptoms caused by insulin reduction. Hyperglycemia, reversing type I diabetes, provides a new option for clinic.

The complete sterioside E / F ring-opening process for synthesizing 26-chloro-3beta, 16beta-diacetyl oxy-22-one-(5-) cholestane (cholestene) belongs to the field of organic synthesis technology. The process includes the first C3-OH acetylation of the material, cheap yamogenin or sisal sapogenin, to obtain acetylated sapogenin; the subsequent selective complete E / F ring-opening of the acetylated sapogenin in acetic anhydride solution at 80 deg.c via introducing dry hydrogen chloride gas to obtain coarse product; and final purification via silica gel column chromatography to obtain the product. The present invention has cheap material, simple operation, easy separation and purification of the product and high yield.

The invention relates to the technical field of genetic engineering, in particular to mycobacterium genetically engineered bacteria and its application in the preparation of 9α,22-dihydroxy-23,24-bisnorcholester-4-en-3-one. The genetically engineered bacterium is three 3-sterone-∆ in mycobacteria 1 ‑Dehydrogenase gene kstd 1, kstd 2 and kstd 3 inactivated, simultaneously overexpressed gene constructs encoding acetyl-CoA acetyltransferase / thiolase and DNA-binding protein, which can selectively produce 9α, 22-dihydroxy-23, 24-dinorcholesterol-4 ‑ene‑3‑ketone reduces the generation of by-product 9‑OH‑AD, greatly improves production efficiency and product quality, makes the product easy to separate and purify, reduces production cost, and reduces environmental pollution at the same time.

The invention discloses a hot and spicy barbecue sauce. The hot and spicy barbecue sauce is free from any spice, essence and preservatives, rich in roasting flavor, prominent in main flavor, long in quality guarantee period and high in tenderization effect. Particularly, the roasting sauce is scientifically compounded. The mouth feel, the flavor and the functional properties ( antibacterial properties, inoxidizability and pharmacology properties) of barbecue foods are improved. Comprehensive flavor substances of food materials under the barbecue condition are collected, so that the barbecue sauce has rich and attractive special barbecue flavor, and the problem that the food materials are free from barbecue flavor before being barbecued, is solved. Besides, a basic sauce and complex enzymeare scientifically compounded, so that the cholesterol level of food materials and auxiliary materials is reduced, and a new functional substance namely 3-oxo-4-cholestene is generated. The possibility that cholesterol is barbecued at high temperature or burnt to generate the carcinogen is prevented, and the safety of the foods is further improved. The prepared barbecue foods are fresh and tenderin meat quality, fresh and delicious in flavor, rich in barbecue flavor, low in cholesterol level and high in food safety. A new shortcut for preparing the barbecue foods with special barbecue flavoris developed.

The invention provides a compound obtained by shifting 5,6-double bond of solasodine and carrying out functional group conversion, namely 3,6-dihydroxyl-22(27)imino-4-furan sterene. The compound has excellent action of resisting cancer cell growth, is high in activity, and can be used as a raw material for preparing anticancer medicaments for resisting leukaemia, colon cancer, rectum cancer, lung cancer and the like. The invention also provides a preparation method of the compound. The preparation method comprises the step of: based on 3 beta,16 beta-diacetoxy-26-chloro-5-cholestene-22-ketone as a raw material, carrying out 3-acetyl alcoholysis, pyridinium chlorochromate carrier reagent oxidation, reduction, amine cyclization and ring-closing reaction.

The invention relates to the technical field of genetic engineering, in particular to a mycobacterium genetically engineered bacterium and its application in the preparation of 22-hydroxy-23,24-bisnorcholester-4-en-3-one. The genetically engineered bacterial strain provided by the present invention greatly reduces the generation of other products such as 4-AD, ADD, selectively produces 22-hydroxyl-23,24-dinorchol-4-en-3-one, and improves production efficiency And product quality, the effect is very significant.

The invention discloses a synthetic method of a polyhydroxy marine steroid (25R)-5 Alpha- cholest-2 Beta and a 3 Alpha and 26-triol, which uses a low-cost Tigogenin as the raw material and obtains (25R)-5 Alpha-cholest-2-ene-16 Beta and 26-diol through the TsCl esterification, elimination reaction and the reduction of zinc powder / ethanol system, obtains (25R)-26-O- t butyl dimethysilyl-5 Alpha- cholest-2-ene through selectively protecting C-26- hydroxyl, C-16-hydroxyl ethlsulphonic acid esterification and lithium aluminum hydride reduction by t-butyl dimethylsilyl chloride, and at last gets the object compound (25R)-5 Alpha- cholest-2 Beta, 3 Alpha and 26-triol through ring-opening under the acid conditions after the epoxidation. The synthetic route of the invention is characterized in that: the synthetic raw material is of low-cost and easy to get; the synthetic route is scientific and reasonable, etc. The target compound has potential antitumor and antivirus activity.

The invention discloses a Bordetella strain and its application in preparing cholesterol oxidase and cholest-4-en-3-one. The strain is Bordetella sp. B4 CGMCC No.2229, which can synthesize extracellular cholesterol oxidase and convert cholesterol into cholest-4-en-3-one. The strain of the invention has the characteristics of short fermentation time and high enzyme production activity, and the extracellular enzyme activity can reach 2000U / L, which has reached or exceeded the international leading level.

The present invention provides a composition comprising a 5'-OH, 3'-TEG cholesteryl synthetic sequence wherein the sequence is SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8. The present invention provides methods of using this composition for induction of response in a cell, including but not limited to inhibition of cellular proliferation, induction of cell cycle arrest, induction of caspase activation, cleavage of poly(ADP-ribose) polymerase, induction of apoptosis or modulation of extracellular matrix-cell interactions, or combinations thereof, in cancer cells or synovial cells, and methods of using this composition for treating disease.

The invention discloses a cholesterol oxidase and application thereof, and belongs to the technical field of enzyme engineering. A genetically engineered bacterium E.coli BL21(DE3)-pET20b(+)-COD of the cholesterol oxidase is firstly constructed and obtained, the enzyme activity of the cholesterol oxidase in a crude enzyme solution obtained through fermentation reaches up to 700 U.L-1, the specificenzyme activity of the purified cholesterol oxidase is 18.5 U.mg-1, the molecular weight of the cholesterol oxidase is about 60,000 kDa, and the cholesterol oxidase has good temperature tolerance andorganic solvent tolerance. An engineering bacterium fermentation solution is utilized for preparing cholest-4-alkene-3-ketone and cholest-4-alkene-3,6-diketone, in a two-phase transformation system taking methylbenzene as an organic phase, the transformation rate of preparing the cholest-4-alkene-3-ketone by taking cholesterol as a substrate is 30%, and the transformation rate of preparing the cholest-4-alkene-3,6-diketone is 45%.

A convenient synthesis method of methylprotodioscin belongs to the organic synthesis technical field. The method comprises the following steps: using the rich and cheap E / F full-ring-opening product of protodioscin, namely 26-choro-3beta,16beta-diacetoxy-22-one-5-cholestene as raw material to perform selective alcoholysis of C3-OAc and protection of C3-OH, then in THF / MeOH mixed solution, using the high selectivity of weak base K2CO3 to close E-ring to form C22-OH, then using methyl iodide to perform methylation to C22-OH, and finally performing a substitution reaction and deprotection of C3-OH. The synthesis of methylprotodioscin is realized in a concise and atom economy manner. The raw material and reagents used in the invention are cheap, the operation is simple, the reaction conditions are mild, the product is easy to separate and purify, the yield is high, especially by using the selective ring-opening reaction and the methylation reaction using the active methylating agent methyl iodide, the construction of furan ring in methylprotodioscin and the methylation of C22-OH can be effectively realized, and by attaching the same or different sugar chains to the C3 and C26 of the methylprotodioscin molecule, methylprotodioscin and multiple analogs can be synthesized. Therefore, the method provides a new synthetic approach for methylprotodioscin and other similar saponins.

The invention discloses a strain of Burkholderia cepacia and its application, belonging to the field of microorganisms. The Burkholderia cepacia ZWS15 was deposited in the China Center for Type Culture Collection on November 6, 2017, with the preservation number CCTCC NO: M2017661, and the preservation address is Wuhan University, Wuhan, China. The bacterium can use cholesterol as the sole carbon source, can grow at 50°C, tolerates 10% organic solvents, and the enzyme activity of cholesterol oxidase in the crude enzyme liquid obtained by its fermentation is as high as 200U / L. Using Burkholderia cepacia ZWS15 fermented crude enzyme solution to catalyze cholesterol in a two-phase system in the presence of organic solvents, the amount of cholest-4-en-3-one collected was about 5-11% of the added amount of substrate %, the amount of cholest-4-ene-3,6-dione is about 10-15% of the amount of substrate added.