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40 results about "Protodioscin" patented technology
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Protodioscin is a steroidal saponin compound found in a number of plant species, most notably in the Tribulus, Trigonella and Dioscorea families. It is best known as the putative active component of the herbal aphrodisiac plant Tribulus terrestris.
Method for extracting protodioscin from semen trigonellae
InactiveCN101255183AHigh puritySimple processSteroids preparationPlant ingredientsProtodioscinSilica gel
The invention provides a process for abstracting protodioscin from fenugreek, comprising: (1) pretreatment of the medicinal materials; (2) ultrasonic extraction; (3) process of extract; (4) purification: purifying and separating the extract by the process of macroporous adsorption resin and silica gel column chromatography. The technical features of the invention comprise: firstly, applying the macroporous adsorption resin technology to the preparation of the abstraction and purification of protodioscin, preparing high-purity protodioscin with a conventional method. The method has a simple process, the production cost is low, and product purity is high.
Owner:NANCHANG UNIV
Health-care cold tea and preparation method thereof
InactiveCN101978844AImprove nutrient utilizationEasy to drinkTea substituesAdditive ingredientProtodioscin
The invention provides health-care cold tea prepared mainly from honeysuckle and Camellia nitidissima and a preparation method thereof. The invention adopts a technical scheme that: ingredients of the health-care cold tea comprise 10 to 90 percent of honeysuckle and 90 to 10 percent of Camellia nitidissima; and the preparation method comprises the step of grinding the honeysuckle and Camellia nitidissima into powder respectively or grinding the honeysuckle and Camellia nitidissima into powder by using a supermicron milling technique, stirring uniformly, and packing by 2 to 10 gram bags. In the invention, the honeysuckle and Camellia nitidissima are ground into powder by the supermicron milling technique, so the nutrient utilization rate is high, and the tea is convenient to drink; and the tea has the effects of clearing heat, detoxifying, resisting bacteria and inflammation, regulating immunity, promoting dieresis and diminishing swelling, is rich various natural nutrients such as tea polyphenol, protodioscin, total flavonoids, tea polysaccharide, tea pigment, caffeine, vitamin and amino acid, contains various trace elements such natural organic germanium (Ge), selenium (Se), molybdenum (Mo), zinc (Zn) and the like, and has the health-care functions of preventing cancer, inhibiting tumor growth, reducing blood fat and cholesterol, preventing atherosclerosis, preventing prostatitis, supplying nutrients, in habiting bacteria and resisting aging, and is suitable for all people.
Owner:蒋科罡
Combiner capable of promoting movement function and sexual function
InactiveCN102578588AImprove fatigue resistanceImprove animal performanceFood preparationSexual functionTribulus terrestris
The invention discloses a combiner capable of promoting the movement function and the sexual function. The combiner comprises 4-48 parts by weight of yohimbine hydrochloride, 5-150 parts by weight of icariin, 20-300 parts by weight of tribulus terrestris protodioscin, 10-600 parts by weight of eurycoma longifolia extract, 10-600 parts by weight of maca extract, 10-500 parts by weight of taurine, 10-100 parts by weight of zinc aspartate, 150-1350 parts by weight of magnesium aspartate and 0.1-5 parts by weight of vitamin B6.
Owner:SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Process for the extraction of furostanolic saponins from fenugreek seeds
Owner:GOEL PAWAN KUMAR
Extract obtained by a commercially viable process for the extraction of furostanolic saponins from fenugreek seeds, in which one of the compounds in the extract is protodioscin
Disclosed is an extract obtained by a commercially viable process for the extraction of furostanolic saponins from fenugreek seeds, in which one of the compounds in the extract is protodioscin. The process includes providing plurality of fenugreek seeds and crushing the same by milling and grinding; performing a primary extraction on the prepared fenugreek seeds using a first hydrophilic polar solvent which is a lower primary aliphatic alcohol followed by ion-exchange chromatography; performing a secondary extraction after ion-exchange chromatography using a composite solvent; and decolorizing the final extract by use of activated charcoal concentration of the extract by heating and vacuum drying sieving the final powdered product.
Owner:GOEL PAWAN KUMAR
Method for testing quality of Discorea nipponica Makino in different places and medicinal materials of same genera
The invention provides a method for testing the quality of Discorea nipponica Makino and medicinal materials of dioscorea in different places. The method comprises the following steps: using medicinal material powder to be tested to prepare sample solutions, testing under a certain chromatographic conditions, and calculating the content of the indexes of each sample through external standard method, and the method is characterized in that dioscin and protodioscin are used as the content determination indexes. Dioscin and protodioscin are the main saponins components of dioscorea medicinal material, are active ingredients of Discorea nipponica Makino for reducing blood fat and are very important to control the quality of medicinal materials and preparations. The invention adopts RP-HPLC analysis method to test the contents of dioscin and protodioscin in Discorea nipponica Makino and medicinal materials of dioscorea in different places; and the method is sensitive, accurate and targeted and can be used to control the quality of medicinal materials, extracts and corresponding preparations.
Owner:深圳市药品检验所
Novel process for the extraction of furostanolic saponins from fenugreek seeds
A novel, commercially viable process for the extraction of high purity (>70%), bioactive furostanolic saponins including protodioscin (>30%), from the seeds of fenugreek (Trigonella foenumgraecum) comprising essentially the steps of powdering of the natural material followed by a first extraction, an ion-exchange chromatographic purification and a second extraction using a novel composite solvent and drying of the product under defined conditions, the entire process being carried out at essentially neutral pH and low temperatures of less than 80° C.
Owner:GOEL PAWAN KUMAR
Ginseng saponin H extract and preparation method thereof
InactiveCN102058644AGood curative effectHigh purityOrganic active ingredientsAntineoplastic agentsProtodioscinTraditional medicine
The invention relates to a traditional Chinese medicine extract and a preparation method thereof, in particular to a Ginseng saponin H extract and a preparation method thereof. In the extract, the content of Ginseng saponin Rh1 is between 1 and 15 percent, the content of Ginseng saponin Rh2 is between 10 and 30 percent, the content of the Ginseng saponin Rh1 and the Ginseng saponin Rh2 is not less than 25 percent and the content of protodioscin is not less than 50 percent based on that of the Ginseng saponin Rh2.
Owner:TIANJIN TASLY PHARMA CO LTD
Ginseng saponin extract and preparation method thereof
InactiveCN102058642AGood curative effectHigh purityOrganic active ingredientsAnalysis using chemical indicatorsProtodioscinTraditional medicine
The invention relates to a traditional Chinese medicine extract and a preparation method thereof, in particular to a Ginseng saponin extract and a preparation method thereof. In the extract, the content of protodioscin is not less than 80 percent based on that of Ginseng saponin Rh2, the content of Ginseng saponin Rh1 and the Ginseng saponin Rh2 is not less than 50 percent, the content of Ginseng saponin Rh1 is between 1 and 20 percent and the content of the Ginseng saponin Rh2 is between 20 and 60 percent.
Owner:TIANJIN TASLY PHARMA CO LTD
Method for comprehensively extracting protodioscin and dioscin from dioscorea nipponica
ActiveCN102875635AHigh product contentReduce manufacturing costSugar derivativesSteroidsDioscorea nipponicaOrganic solvent
The invention aims to compressively develop dioscorea nipponica raw materials, and provides a method for comprehensively extracting protodioscin and dioscin from dioscorea nipponica. The technical concept is shown by the following steps: 1, crushing raw materials, synchronously extracting protodioscin and dioscin through an organic solvent; 2, combining the extracting liquid, concentrating to reach proportion of about 1.12, filtering, dissolving the obtained precipitate through an ethanol solution, then separating dioscin from the precipitate (preferably utilizing acetone), and filtering to obtain a dioscin product; and 3, extracting the mother liquor filtered in step 2, concentrating and parching the extracting liquid to obtain a rough product, and recrystallizing the rough product to obtain the protodioscin product. With the adoption of the method provided by the invention, the problem in the prior art that the resource is wasted because two products cannot be comprehensively developed can be overcome, the extracting step is optimized, the producing cost is reduced, and the operability is improved.
Owner:SHAANXI JIAHE PHYTOCHEM
Methyl or ethyl protodioscin chemical synthesis method
The present invention relates to the simple chemical process of synthesizing methyl or ethyl protodioscin with dioscin as material. The process includes the following steps: 1. protecting trisaccharide chain in dioscin; 2. oxidizing the completely protected dioscin; 3. introducing place 26 glucosyl radical; 4. eliminating protecting epoxy radical; 5. reducing place 16 carbonyl radical selectively with reductant; 6. eliminating all the protecting radicals under alkaline or hydrogenating condition to obtain methyl or ethyl protodioscin.
Owner:SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
Method for extracting protodioscin from fenugreek seeds
InactiveCN104784246AHigh puritySimple extraction processMetabolism disorderAntineoplastic agentsAlcoholProtodioscin
The invention discloses a method for extracting protodioscin from fenugreek seeds. The method comprises the following the steps: (1) pressing the fenugreek seeds with a screw-type oil expeller; taking off lipid compositions to obtain fenugreek seed cake meal; (2) extracting the fenugreek seed cake meal with purified water twice, filtering the extracting solution to obtain filtrates; combining the filtrates; (3) conducting purification on the combined filtrate with a CAD-40 macroporous resin column, carrying out washing with water, using ethyl alcohol with a concentration of 70% for elution, carrying out decompressing and concentrating till no alcohol exists, and carrying out spraying and drying to obtain an extraction containing protodioscin. The method for extracting protodioscin from the fenugreek seeds is simple in extracting operation, and low in energy consumption and cost, and has the advantage that the purity of effective components in the extraction can be improved.
Owner:ZHEJIANG HUISONG PHARMA
Composition capable of promoting movement function and sexual function
InactiveCN102578588BImprove fatigue resistanceImprove animal performanceFood preparationSexual functionMedicine
Owner:SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
Quantitative analysis method of six chemical components in Chinese herbal medicine compound preparation using rhizoma dioscoreae nipponicae and acanthopanax roots as Chinese herbal medicines
ActiveCN104502485AImprove quality controlImprove accuracyComponent separationChlorogenic acidEleutherococcus senticosus
The invention discloses a quantitative analysis method of six chemical components in a Chinese herbal medicine compound preparation using rhizoma dioscoreae nipponicae and acanthopanax roots as Chinese herbal medicines. The quantitative analysis method comprises the following steps: (1) preparing a mixed control solution; (2) preparing a first test sample solution and a second test sample solution; (3) determining contents, namely, respectively taking the mixed control solution, the first test sample solution and the second test sample solution, performing gradient elution under high performance liquid chromatography detection conditions of using C18 bonded silica gel as a chromatographic column of a filling agent and acetonitrile-formic acid solution as a mobile phase, performing high performance liquid chromatography detection by combining a diode array detector and an evaporative light-scattering detector of detectors to obtain high performance liquid chromatograms of the mixed control solution, the first test sample solution and the second test sample solution, and determining the contents of dioscin, pseudoprotodioscin, protodioscin, chlorogenic acid, syringin and isofraxidin in a test sample by using an external standard method. The quantitative analysis method is simple, convenient and rapid to operate, high in accuracy and excellent in repeatability, and can be used for improving the quality control level of a product.
Owner:TIANJIN UNIV +1
Health protection tea and preparation method thereof
The invention relates to health protection tea and a preparation method thereof. The invention provides health protection tea prepared from camellia nitidissima and Chinese globeflower flower serving as main materials, and a preparation method thereof. The technical scheme is that: the health protection tea comprises 90 to 10 weight percent of camellia nitidissima and 10 to 90 weight percent of Chinese globeflower flower; and there are two preparation methods: 1, decocting the camellia nitidissima and the Chinese globeflower flower to prepare an extract, and adding a proper amount of sugar, preservatives and water to prepare a drink; and 2, respectively crushing the camellia nitidissima and the Chinese globeflower flower into powder, or making micropowder by using super-micro pulverization technology, uniformly stirring and packaging into bags, wherein each bag contains 2 to 10g of powder. The health protection tea has the effects of clearing heat and releasing toxin, inhibiting bacteria and resisting inflammation, regulating immunity, and promoting urine and diminishing swelling, is rich in various natural nutrients such as tea polyphenol, protodioscin, general flavones, tea polysaccharide, tea pigment, caffeine, amino acid, and various trace elements such as natural organic germanium (Ge), selenium (Se), molybdenum (Mo) and zinc (Zn), has health care effects of preventing cancers, inhibiting tumor growth, reducing blood fat and cholesterol, preventing atherosclerosis, preventing prostatitis, supplementing nutrition, inhibiting bacteria, resisting ageing and the like, and is suitable for wide consumers.
Owner:蒋科富
High-frequency induction and genetic transformation method for pear hairy roots of Agrobacterium rhizogene-mediated balsam
InactiveCN101985633AVector-based foreign material introductionAngiosperms/flowering plantsBiotechnologyBalsam
The invention relates to a high-frequency induction and genetic transformation method for pear hairy roots of Agrobacterium rhizogene-mediated balsam, comprising the following steps: selecting R1000 agrobacterium rhizogene strains to infect the stems or scutellum of seedlings in test tubes with the seedling age of 10-20 days, co-culturing in an MS culture medium containing IBA5.0 for 1-2 days to obtain co-cultured explants, and performing bacteria-free culture on the explants on a 1 / 2MS0 solid medium containing cephalosporin with the concentration of 500mg L<-1> for 8-10 days to obtain the balsam pear hairy roots. The balsam pear hairy roots acquired by the invention has the rooting frequency and rooting density, which respectively reaches 84.2% and 12.3%, and the total content of balsam pear protodioscin in the balsam is 33.43mg / g, which is respectively 69.1%, 48.6% and 49.8% higher than those in the root, stem and leaf of the seedling in a test tube. The method of the invention lays a foundation for breeding new fine-quality and high-yield balsam pear varieties and utilizing Ri plasmid as a vector to perform genetic transformation, and provides an effective path for adopting the balsam pear hairy roots to culture and biosynthesize medical components such as balsam pear saponin and the like.
Owner:毛清黎
Thin-layer chromatography identification method of radix asparagi decoction piece in ophiopogon and asparagus decoction composition
The invention discloses a thin-layer chromatography identification method of a radix asparagi decoction piece in an ophiopogon and asparagus decoction composition. The identification method is established by utilizing a thin-layer chromatography test method and adopting a lower-layer solution of trichloromethane-methyl alcohol-water as a developing solvent after the radix asparagi decoction piececomponent in ophiopogon and asparagus decoction, a radix asparagi contrast medicinal material, protodioscin and a protodioscin contrast product are mixed. The identification method is high in accuracyrate, high in specificity and wide in using range.
Owner:葵花药业集团(襄阳)隆中有限公司
Novel antidiabetic furostanolic saponin rich (FSR) fraction from fenugreek seeds
InactiveUS20120071427A1Effective treatmentEasily and conveniently formulatedBiocideOrganic active ingredientsOral treatmentAdditive ingredient
The present invention discloses a novel anti-diabetic composition extracted from fenugreek seeds. The same comprises a furostanolic-saponin-rich fraction (>70%) with approximately 30% protodioscin as one of the bioactive components. Pre-clinical studies in rats indicated significant glucose lowering effect of the fraction (31.5%) as compared to control after two weeks of oral treatment. Clinical studies in human volunteers indicated suitability of a dosage form of 500 mg given once or twice daily as anti-diabetic agent either alone or in combination with standard, synthetic anti-diabetic drugs such as metformin and glipizide in controlling plasma glucose levels.
Owner:GOEL PAWAN KUMAR
Method for extracting protodioscin from dioscorea opposita plant
ActiveCN105801665AEffective bondageEfficient releaseSteroidsBulk chemical productionProtodioscinCellulase
The invention relates to a method for extracting protodioscin from a dioscorea opposita plant, in particular to a method for extracting high-purity protodioscin by adopting a compound enzyme of cellulase and amylase to perform pretreatment and utilizing the characteristics of CO2 at a supercritical state. The method has the advantages of simple technological flow, high extraction efficiency, complete active ingredients and no environmental pollution.
Owner:HUNAN AGRICULTURAL UNIV
Ginseng saponin H extract and preparation method thereof
InactiveCN102058643AGood curative effectHigh purityOrganic active ingredientsComponent separationMedicineProtodioscin
The invention relates to a traditional Chinese medicine extract and a preparation method thereof, in particular to a Ginseng saponin H extract and a preparation method thereof. In the extract, the content of protodioscin is not less than 60 percent based on that of Ginseng saponin Rh2 and the content of the Ginseng saponin Rh1 and the Ginseng saponin Rh2 is not less than 30 percent.
Owner:TIANJIN TASLY PHARMA CO LTD
Method for preparing protodioscin
InactiveCN104861035AHigh purityThe preparation process is simpleSteroidsProtodioscinColumn chromatography
The invention belongs to the technical field of medicine, relates to a method for separation extraction of a compound in the chemical field and especially relates to a preparation method of a plant natural pharmacological active substance protodioscin. The method utilizes a rhizoma dioscoreae nipponicae medical material as a raw material and utilizes a column chromatography method to purify protodioscin. The method has the advantages of simple refining process, operation easiness, high purification efficiency and short refining time.
Owner:JIANGSU YONGJIAN PHARMA TECH CO LTD
Method for preparing protodioscin
InactiveCN106188178AHigh purityThe preparation process is simpleSugar derivativesSugar derivatives preparationDioscorea nipponicaProtodioscin
The invention belongs to the technical field of medicines, relates to a separation and extraction method of compounds in the field of chemistry, and concretely relates to a method for preparing a plant natural pharmacological active substance protodioscin. The method is characterized in that a Dioscorea nipponica Makino medicinal material is adopted as a raw material, and a column chromatography technology is adopted to purify protodioscin. The above purifying technology has the advantages of simplicity, easiness in operation, high purifying efficiency and short purifying time.
Owner:宁波浚源国际贸易有限公司
Multifunctional jerusalem artichoke capsules for treating diabetes by nourishing pancreas, eliminating root causes and suppressing cancer
InactiveCN101972425AWide variety of sourcesImprove spleen and stomach functionMetabolism disorderFish material medical ingredientsSide effectCancer cell
The invention discloses multifunctional jerusalem artichoke capsules for treating diabetes by nourishing pancreas, eliminating root causes and suppressing cancer. The capsules comprise the following basic components by weight: 2g of jerusalem artichoke powder, 2g of lychee seed, 2g of longan seed, 1.5g of pawpaw seed, 1.5g of lalang grass rhizome, 1.5g of bitter gourd, 1g of eel, 0.5g of manyflower solomonseal rhizome and 0.5g of yam. Through mutual compatibility effect of the raw materials, the functions of spleen and stomach can be adjusted, the immunity of body is improved, and directional nutrient components such as protodioscin of the lychee seed, saponin fat and tannin of the longan seed, ferment of the pawpaw seed and the like can be utilized to nourish the pancreas and eliminate the root causes so as to fulfill the aim of suppressing and lowering glucose; and the saponin and alkaloid component included in the capsules can greatly suppress and damage mitosis of cancer cells, so that the cancer cells cannot divide but even die. The used raw materials have wide sources and low cost and do not have side effect on human bodies.
Owner:孙吉良
Ethanol extract of Dulong Chonglou and its application in pharmaceuticals and its chemical constituents separation and identification method
ActiveCN107446014BStrong inhibitory activityOrganic active ingredientsComponent separationProtodioscinPolyphyllin I
Owner:KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
Ethanol extract of paris dulongensis H. Li&Kurita, application of ethanol extract to pharmacy and separation and identification method of chemical components of ethanol extract
ActiveCN107446014AStrong inhibitory activityOrganic active ingredientsComponent separationBeta-EcdysoneProtodioscin
The invention discloses an ethanol extract (PDE4) and total saponin (PDE5), which have anticancer activity, from paris dulongensis H. Li&Kurita, preparation methods of the ethanol extract (PDE4) and the total saponin (PDE5) and application of the ethanol extract (PDE4) and the total saponin (PDE5) to the preparation of a medicine for treating or preventing leukemia, a liver cancer, a lung cancer, a mammary cancer and a colon cancer, and provides a separation and identification method of chemical components in the PDE 5. Polyphyllin V, polyphyllin I, beta-ecdysone, protodioscin, parisaponin I and ginsenoside Rg1 are separated from the PDE5 and identified by adopting a separation method of chromatographic column chromatography and semi-preparative high performance liquid chromatography and an identification method of a mass spectrum and a nuclear magnetic resonance wave spectrum. The separation and identification method is used for clarifying main chemical components of the PDE5 for the first time, can be used for simply ad quickly obtaining the chemical components from the PDE5 through a provided method, and is used for reconstructing a quality control standard for the paris dulongensis H. Li&Kurita, the extract PDE4 of the paris dulongensis H. Li&Kurita or the total saponin PDE5 of the paris dulongensis H. Li&Kurita.
Owner:KUNMING INST OF BOTANY - CHINESE ACAD OF SCI +1
Application of total saponin of Dioscorea in preparation of drugs for controlling diabetic nephropathy
InactiveCN105267564ALower blood sugarConsiderable developmentMetabolism disorderUrinary disorderProtodioscinDioscorea
The invention discloses a preparation method and application of an effective part of total saponin of Dioscorea. The effective part is composed of protodioscin, methyl protodioscin, gracillin, protogracillin, dioscin and diosgenin, and can be used alone or in a mixture with a pharmaceutically acceptable carrier to prepare the drugs for controlling diabetic nephropathy. Efficacy experiments show that the effective part of total saponin of Dioscorea can give the evident reduction in blood glucose, kidney hypertrophy level and urine protein discharge and delaying of urine protein formation, and is functional in repairing kidney injury caused by diabetics.
Owner:CHINA PHARM UNIV
Method for improving tyrosinase inhibition activity
ActiveCN110151580ARelatively low activityEnhanced inhibitory effectOrganic active ingredientsCosmetic preparationsGallic acid esterTyrosine
The invention discloses a method for improving tyrosinase inhibition activity, and belongs to the technical field of deep processing of fruits and vegetables. According to the method, a tyrosinase inhibitor is prepared from phenolic acid with the concentration of 0.6-1.8 mg / mL and saponin with the concentration of 0.5-1.5 mg / mL; the phenolic acid is prepared from three of quercetin, cinnamic acid,ferulic acid, gallic acid and isorhamnetin; the use amount ratio of any three components of the phenolic acid is 1:(1-10):(1-10); the saponin includes one of protodioscin, dioscin and sarsasapogenin.According to the method, the tyrosinase inhibitor is formed by compounding polyphenol and the saponin, the minimum relative activity of treated tyrosinase can reach 5%, and the polyphenol with the three components can generate a synergistic effect; the saponin can be deglycosylated to generate polyphenol polymers and other substances with a high tyrosinase inhibition function at high temperature,and therefore the inhibition effect of the tyrosinase inhibitor on the tyrosinase is greatly improved.
Owner:JIANGNAN UNIV
Ginseng saponin extract and preparation method thereof
InactiveCN102058642BGood curative effectHigh purityOrganic active ingredientsAnalysis using chemical indicatorsProtodioscinTraditional medicine
The invention relates to a traditional Chinese medicine extract and a preparation method thereof, in particular to a Ginseng saponin extract and a preparation method thereof. In the extract, the content of protodioscin is not less than 80 percent based on that of Ginseng saponin Rh2, the content of Ginseng saponin Rh1 and the Ginseng saponin Rh2 is not less than 50 percent, the content of Ginseng saponin Rh1 is between 1 and 20 percent and the content of the Ginseng saponin Rh2 is between 20 and 60 percent.
Owner:TIANJIN TASLY PHARMA CO LTD
Methyl or ethyl protodioscin chemical synthesis method
Owner:SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI
A method for enhancing tyrosinase inhibitory activity
ActiveCN110151580BRelatively low activityEnhanced inhibitory effectCosmetic preparationsOrganic active ingredientsGallic acid esterTyrosine
Owner:JIANGNAN UNIV
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