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Ginseng saponin extract and preparation method thereof

A technology of ginsenosides and total ginsenosides is applied in the direction of testing pharmaceutical preparations, drug combinations, pharmaceutical formulations, etc., and can solve the problems of many by-products, difficult industrialized production, and low yield of active ingredients.

Inactive Publication Date: 2011-05-18
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The effect of separating ginsenoside H in the prior art is not good, the yield of active ingredients is low, and there are many by-products, so it is difficult to industrialize production

Method used

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  • Ginseng saponin extract and preparation method thereof
  • Ginseng saponin extract and preparation method thereof
  • Ginseng saponin extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1 Preparation of Ginsenoside H Extract

[0085] Step 1. Extract the American ginseng leaves with water twice for 2 hours each time. Add 10 times the amount of water each time. After being concentrated, the extract is passed through the D101 macroporous adsorption resin column, first eluted with water, and eluted until the effluent is nearly colorless, and then used Elute with 85% ethanol, collect the 85% ethanol eluate and concentrate to dryness to obtain total saponin;

[0086] Step 2. Dissolve 10kg of total saponins and 10kg of sodium hydroxide in 60kg of glycerin, heat, and control the reaction temperature at 230°C for a reaction time of 30min to obtain alkali degradation products.

[0087] Step 3. The alkali degradation product is heated with 95% ethanol to dissolve it, and then mixed with 2 times the amount of silica gel, dried and crushed and sieved to perform silica gel column chromatography. The ratio of alkali degradation product to chromatography silica gel i...

Embodiment 2

[0088] Example 2 Preparation of Ginsenoside H Extract

[0089] Step 1. Extract American ginseng leaves with water 3 times, 3 hours each time, add 10 times the amount of water each time, the extract passes through the D101 macroporous adsorption resin column, first eluted with water, eluted until the effluent is nearly colorless, and then used 50% Ethanol elution, collect the eluate, concentrate to dryness to obtain total saponin;

[0090] Step 2. Dissolve 10 kg of total saponins and 10 kg of potassium hydroxide in 60 kg of glycerol, heat, and control the reaction temperature at 150° C. and the reaction time for 60 minutes. Get alkaline degradation products,

[0091] Step 3. The alkali degradation product is heated with 85% ethanol to dissolve it, and 3 times the amount of silica gel is stirred, dried and crushed and sieved to perform silica gel column chromatography. The ratio of the alkali degradation product to the chromatography silica gel is 1:5. Elute with a solution system wi...

Embodiment 3

[0092] Example 3 Preparation of Ginsenoside H Extract

[0093] Step 1. The American ginseng is extracted with water, and the extract is passed through the AB-8 macroporous adsorption resin column, first eluted with water, and eluted until the effluent is nearly colorless, followed by elution with 95% ethanol, and collect the 95% ethanol eluate. Concentrate to dryness to obtain total saponins;

[0094] Step 2. Dissolve 10 kg of total saponins and 10 kg of sodium carbonate in 60 kg of glycerol, heat, and control the reaction temperature at 200° C. and the reaction time for 20 minutes. Get alkaline degradation products,

[0095] Step 3. Heat the alkali degradation product with 95% ethanol to dissolve it, stir in 2 times the amount of silica gel, dry and smash through a sieve, and perform silica gel column chromatography. The ratio of alkali degradation product to chromatography silica gel is 1:10. The volume ratio is chloroform-methanol (8:1) solution system for elution, thin-layer TL...

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Abstract

The invention relates to a traditional Chinese medicine extract and a preparation method thereof, in particular to a Ginseng saponin extract and a preparation method thereof. In the extract, the content of protodioscin is not less than 80 percent based on that of Ginseng saponin Rh2, the content of Ginseng saponin Rh1 and the Ginseng saponin Rh2 is not less than 50 percent, the content of Ginseng saponin Rh1 is between 1 and 20 percent and the content of the Ginseng saponin Rh2 is between 20 and 60 percent.

Description

Technical field [0001] The invention relates to a traditional Chinese medicine extract and its preparation, in particular to a ginsenoside extract and its preparation. Background technique [0002] Ginsenoside H is the secondary saponins produced by alkali-catalyzed hydrolysis of ginsenosides, and the effective part-H group saponins are refined by silica gel column chromatography. The main components are ginsenosides Rh1, Rh2 and Rh3. Rh2 is the main component of the drug and the main anti-cancer active component of the drug. Pharmacodynamic studies have shown that ginsenoside Rh2 has a significant and specific inhibitory effect on the proliferation of cancer cells such as liver cancer, melanoma B16, cervical cancer and sarcoma S180. Its mechanism of action is: in the process of cancer cell proliferation, the action of ginsenoside Rh2 stops cancer cells in a certain proliferation period and promotes their reversion to normal cells. Group H saponins containing ginsenosides Rh1, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61K9/20G01N31/22G01N30/90G01N30/02G01N33/15G01N21/31A61P35/00A61K31/704A61K125/00A61K127/00
Inventor 宋兆辉张兰兰黄芝娟林万里蔡楠周水平朱永宏
Owner TIANJIN TASLY PHARMA CO LTD
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