Method for extracting protodioscin from semen trigonellae

A technology of protodioscin and fenugreek, which is applied in the field of extracting active ingredients of plants, can solve the problems of low product purity, complex equipment, and high technical difficulty, and achieve the effects of simple process, low production cost, and high product purity

Inactive Publication Date: 2008-09-03
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although some progress has been made in the chemical synthesis of protodioscin, the technical difficulty is high, the equi

Method used

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  • Method for extracting protodioscin from semen trigonellae

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Embodiment 1

[0019] Take 5 grams of fenugreek medicinal material, wash away the sand with water, dry below 100°C to constant weight, then grind into powder, pass through a 40-mesh sieve, and extract 3 times, adding 300 ml of 75% ethanol for each extraction, time 15min, 15min, 15min in sequence, control pH=7 for each extraction, filter after extraction, combine the filtrates, distill the extract under reduced pressure, dissolve the concentrated solution obtained by the reduced-pressure distillation with an appropriate amount of water, centrifuge, filter, and separate the water phase Partial HP-20 macroporous adsorption resin chromatography separation, the amount of resin is 40 grams, the ratio of diameter to height is 1:12, the adsorption volume flow rate of the upper column is 4BV / h, then add 3 times the volume of resin column bed water, and the flow rate is controlled at 10BV / h washes away impurities, then elutes protodioscin with 70% ethanol of 3 times the volume of the resin column bed,...

Embodiment 2

[0021] Take 10 grams of fenugreek medicinal material, wash away the sand with water, dry below 100°C to constant weight, then grind into powder, pass through a 40-mesh sieve, and extract 3 times, adding 600 ml of 75% ethanol for each extraction, time 15min, 15min, 15min in sequence, control pH=7 for each extraction, filter after extraction, combine the filtrates, distill the extract under reduced pressure, dissolve the concentrated solution obtained by the reduced-pressure distillation with an appropriate amount of water, centrifuge, filter, and separate the water phase Partial HP-20 macroporous adsorption resin chromatography separation, the amount of resin is 80 grams, the ratio of diameter to height is 1:12, the adsorption volume flow rate of the upper column is 4BV / h, then add 3 times the volume of resin column bed water, and the flow rate is controlled at 10BV / h washes away impurities, then elutes protodioscin with 70% ethanol of 3 times the volume of the resin column bed...

Embodiment 3

[0023] Take 20 grams of fenugreek medicinal material, wash away the sandy soil with water, dry below 100°C to constant weight, then grind into powder, pass through a 40-mesh sieve, and extract 3 times, adding 1200 ml of 75% ethanol for each extraction, time 15min, 15min, 15min in sequence, control pH=7 for each extraction, filter after extraction, combine the filtrates, distill the extract under reduced pressure, dissolve the concentrated solution obtained by the reduced-pressure distillation with an appropriate amount of water, centrifuge, filter, and separate the water phase Partial HP-20 macroporous adsorption resin chromatographic separation, the amount of resin is 160 grams, the ratio of diameter to height is 1:12, the adsorption volume flow rate of the upper column is 4BV / h, then add 3 times the resin column bed volume water, and the flow rate is controlled at 10BV / h washes away impurities, then elutes protodioscin with 70% ethanol of 3 times the volume of the resin colu...

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Abstract

The invention provides a process for abstracting protodioscin from fenugreek, comprising: (1) pretreatment of the medicinal materials; (2) ultrasonic extraction; (3) process of extract; (4) purification: purifying and separating the extract by the process of macroporous adsorption resin and silica gel column chromatography. The technical features of the invention comprise: firstly, applying the macroporous adsorption resin technology to the preparation of the abstraction and purification of protodioscin, preparing high-purity protodioscin with a conventional method. The method has a simple process, the production cost is low, and product purity is high.

Description

technical field [0001] The invention relates to the extraction of effective plant components, in particular to a method for extracting protodioscin from fenugreek. Background technique [0002] Protodioscin (protodioscin) belongs to steroidal saponins (steroidal saponins), is a class of saponins combined with C-27 steroidal compounds and sugar chains, also known as furansterol saponins, and is colorless needle crystals (water) at room temperature. mp is 190~196°C (decomposition), molecular formula is C 51 h 84 o 22 , the molecular weight is 1049.21, and its structure is as follows: [0003] [0004] Protodioscin has strong biological activity, such as enhancing male sexual function, poisoning and killing various cancer cells, lowering blood fat, and anti-leukemia. The biological activity of this substance has attracted extensive attention of Chinese and foreign scholars, and many scholars have carried out fruitful work on the biological activity, pharmacological effec...

Claims

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Application Information

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IPC IPC(8): C07J71/00C07J75/00A61K36/48
Inventor 余淑娴郭青峰郑湘娟洪三国罗冬梅
Owner NANCHANG UNIV
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