606results about "Steroids preparation" patented technology

The invention relates to a method for extracting squalene from vegetable oil deodorized distillate. A process method of combining molecular distillation with extraction and crystallization is used to extract high-concentration squalene and recover natural vitamin E and phytosterol with a certain purity from vegetable oil deodorized distillate. The method comprises the following steps of: carryingout a saponification reaction of the raw material; twice molecularly distilling unsaponifiable matter obtained by extraction; using the distillate obtained during the second distilling as a raw material for cold crystallization to obtain phytosterol; recovering squalene and mixed tocopherol from the filtrate; and extracting with multi-stage solvents to respectively enrich squalene and vitamin E in two phases. The process has simple flows, low-prices raw material and higher separation efficiency and recovery rate and comprehensively utilizes useful contents in the deodorized distillate.

The present invention provides a process for preparation of highly purified mogrosides mixture from low purity mogrosides mixture. The process comprises providing a mixture of low purity mogrosides, dissolving the low purity mogrosides mixture in water or an aqueous alcohol solution to form an initial solution of mogrosides, passing the initial solution through a column system, wherein the column system comprises a plurality of columns, and each column is packed with a sorbent having different affinities to impurities and mogrosides so that one or more columns retains more mogrosides than other columns, washing the columns to remove impurities with an acidic aqueous solution, a basic aqueous solution, and an aqueous alcoholic solution successively, eluting the columns with an aqueous alcohol solution that contains higher alcohol content than the aqueous alcohol solution used in the washing step, wherein the eluate from the columns with high content of mogrosides are combined, and drying the combined eluate to obtain high purity mogrosides with the content of the total mogrosides are more than 70% (w/w). The present invention also provides a sweetener mixture and product comprising high purity mogrosides.

The invention is a method of preparing glossy ganoderma triterpenic acid and amylase by glossy ganoderma, crushing the dry glossy ganoderma seeds (water content 5-8%) into particles; or cutting into slices, then putting raw material in a high-pressure extraction kettle, then injecting CO2 and polar agent in the extraction kettle by a supercritical CO2 high-pressure pump and a polar agent pump, respetively, where the extracting pressure of the high-pressure pump is 25-50 MPa, the injection quantity of the polar agent in bulk percent is 10-20%, the temperature is 35-80 deg.C, and the extracting time 2-4 hours, obtaining the extract of glossy ganoderma triterpenic acid; adding water by 8-16 times in the residues, heating to 80-100 deg.C, extracting for 1-2 hours, obtaining the extract of glossy ganoderma amylase. In the products prepared by this invention, the content of glossy ganoderma triterpenic acid is up to 60.2% (by HPLC detection); that of glossy ganoderma amylase 36% (by UV spectral detection).

Production of unsaturated fatty acid plant sterol ester is carried out by alcoholysis reacting for glycerin trilaurate of vegetable oil under action of methanol and catalyst to obtain fatty acid methyl ester, ester exchange reacting for fatty acid methyl ester and plant sterol under action of catalyst, removing un-reacted methanol and fatty acid methyl ester to obtain crude unsaturated fatty acid plant sterol ester, de-coloring and deodorizing to obtain the refined final product. It's cheap, harmless and non-toxic and can be used in food-industry production.

The invention provides a preparation method of a 7-keto-6[alpha]-alkyl cholanic acid derivative. According to the preparation method provided by the invention, a 7-keto-6[beta]-alkyl cholanic acid derivative, as shown by a formula II, is used as a raw material, and the 7-keto-6[alpha]-alkyl cholanic acid derivative is prepared by converting a 6[beta] configuration into a 6[alpha] configuration under an acid or alkali condition. The invention also provides a 7-keto-6[beta]-alkyl cholanic acid derivative and an application thereof in preparation of 3[alpha],7[alpha]-dihydroxy-6[alpha]-alkyl-5[beta]-cholanic acid. The preparation method provided by the invention is simple and convenient, and is high in configuration conversion rate, and the product, the 7-keto-6[alpha]-alkyl cholanic acid derivative, is easy to purify, so that the purification difficulty for preparing the 3[alpha],7[alpha]-dihydroxy-6[alpha]-alkyl-5[beta]-cholanic acid is reduced.

The invention discloses a novel glycyrrhetinic acid derivative, and a preparation method and medicinal uses thereof. The glycyrrhetinic acid derivative is a compound prepared by coupling nitric oxide donor of furazan nitrogen oxides and glycyrrhetinic acid through ester bonds or amide bonds. As proved by pharmacological experiments, the glycyrrhetinic acid derivative has superior antitumor effectand can be used for the preparation of antitumor medicines.

The invention discloses a chemical synthesis method of methylpredmisolone. Fungi oxide is adopted as the initial material and methylpredmisolone is obtained after prevotella oxidation, bromine application, bromine removal, 6-bit methylenation, 6-bit methylation, ketal protection, 11- bit reduction, ketal hydrolization, 1 and 2 bit dehydrogenation, 21-bit iodine application, 21-bit permutation and 21-bit hydrolytic reaction. The flow of the synthesis method of the invention is reasonable, the yield rate and the purity of the intermediate products are high, the reaction condition is temperate, the by-products in the end product are few, the purity is high (reaching above 98 percent), the industrialization is easy to be realized, thereby the method has high application value.

The invention relates to field of natural medicine and medicinal chemistry, in particular to novel pentacyclic triterpene and melbine salt I of the derivative thereof. The salt compound can be used for preparing medicaments curing diabetes mellitus and complicating disease thereof, cerebral ischemia, angiocardiopathy, atherosclerosis, hepatitis, fatty liver and metabolic syndrome or tumour, descendens blood fat drugs and anti-obesity drugs. The invention also relates to the preparation method of the salt compound.

The invention relates to a novel technology for oxosynthesis of pregnane 11-bit ketonic group, which is characterized in that a compound in a formula 2 uses piperidine nitroxide free-radical as an oxidation catalyst under the condition of an organic solvent, and uses positive valence halide as an oxidant to react to generate a compound in a formula 1.

The invention relates to a method for preparing fatty acid phytosterol esters, which comprises the following step of: esterifying fatty acid and phytosterol in the presence of one or more catalysts such as alumina, aluminum hydroxide, aluminum salt (such as aluminum chloride, aluminum sulfate, aluminum silicate and the like), oxide of other elements in IIIA family, phosphorus pentoxide, phosphoric acid, phosphate (such as sodium dihydrogen phosphate, dipotassium phosphate and the like), a molecular sieve and silica gel in effective catalysis amount. The invention also relates to the fatty acid phytosterol esters prepared by the method, and foods, soft capsules and cosmetics containing the phytosterol esters.

The invention discloses a method to manufacturing high purity chemocholic acid from fowl bile that belongs to refinement chemical and medicine field. It includes the following steps: a. saponification reaction: mixing the fowl bile and sodium hydroxide and reacting for 20-30 hours under the certain temperature; b. neutral reaction: adding dilute hydrochloric acid after saponification reaction and gaining raw cholic acid after filtering; c. column chromatography: adding dilute sodium hydroxide into raw cholic acid and injecting into chromatography column, uniting the eluent; super filtering: taking super filtering by super filtering film; e. gaining chemocholic acid that the purity is over 96% after adding dilute acid, filtering and drying. The invention has simple technology, high purity, low cost and environment protection.

The present invention is a method for preparing triterpenoids such as 2-cyano-3,12-dioxoolean-1,9-dien-28-methyl ester and derivatives thereof from oleanic acid, ursolic acid, betulinic acid, sumaresinolic acid or hederagenin.

The invention belongs to the biochemical industry field, particularly relates to a method for extracting natural phytosterin from residual oil of vegetable fat deodorizing distillate. The method comprises the steps of transforming the sterol ester and the glyceride in the residual oil into free sterol and aliphatic acid by catalyzing and decomposing and extracting natural phytosterin by cool resolving and filtering. The filtrate contains a large amount of aliphatic acid so the filtrate can be used to prepare surface active agent or biodiesel. The invention not only changes the discharged residual oil from waste residue into valuable but also solves the problem that China is lack of natural phytosterin resources, with excellent social benefit, environmental benefit and economic benefit. The invention has simple process, easy operation, excellent controllability, low manufacturing cost, industrialization and wide application prospect.

This invention discloses a method for extracting high-purity cholesterol from lanolin. The method comprises: (1) dissociating cholesterol from lanolin by transesterification reaction; (2) performing short-path distillation to obtain cholesterol crude product; (3) purifying by silica gel column chromatography; (4) crystallizing to obtain refined cholesterol with purity higher than 95% and yield higher than 70%. The method has such advantages as high product consumption, little pollution, simple process and easy operation, thus is suitable for mass production.

The invention relates to a novel process for preparing tea saponin, which integrates solvent extraction and selective separation. The steps are as follows: a. extraction and absorption: adding proper amount of the defatted cake of degreased oil-tea into a sorbitic extractor and adding macroporous absorption resin into the round bottom flask with low-concentration organic solvent, which is then heated up and back flowed; b. absorption stripping: addubg the macroporous absorption resin which has absorbed tea saponin into the solution of high-concentration organic solvent, which is heated up and back flowed in the round bottom flask to obtain the solution of tea saponin through filtering; c. obtaining the products of tea saponin by decoloring and spray drying the solution of tea saponin. The invention has the advantages that macroporous absorption resin is used to absorb tea saponin simultaneously while the tea saponin is extracted and solvent extraction and selective separation are integrated so that the manufacturing process is shortened, the energy consumption and production cost are reduced, the pollution during the process is decreased, the extracting solution is recycled and the consumption of organic solvent is reduced substantially, which can make full use of and fully develop the resource of defatted oil-tea cake.

The invention discloses a method for separating triptolide from thunder god vine leaves. Thunder god vine leave powders are extracted by ethanol, the ethanol is firstly distributed by an ethyl acetate-water system, the top phase is steamed to obtain ethyl acetate extractive matter, the extractive matter is then distributed by a sherwood oil- methanol system, and the bottom phase is steamed to obtain methanol extractive matter. The sampling amount of column chromatography step is reduced by the twice distribution. The eluent is steamed by simple column chromatography to obtain the crude product of triptolide, the crude product of triptolide is distributed by a quaternionic biphase system, impurities such as coloring matter, and the like are removed, the sampling amount of high-speed reverse-current coloring matter step is reduced, and the competitive product of triptolide is obtained by refining high-speed reverse-current coloring matter. The purity of the competitive product of triptolide is about 95%, and the yield is 0.008% higher than leaves. The solvent in the method does not contain chloro-solvent which is harmful to environment and human bodies.

The invention provides a method of separating soapnut saponin with the foam separation method, in particular a novel method of separating soapnut saponin from crude soapnut saponin extract with the foam separation method. The soapnut saponin with the high purity of 89-94 percent is obtained by the pretreatment, the foam separation, the vacuum concentration and the freeze drying of the crude soapnut saponin extract. Compared with the method of separating the soapnut saponin with resin, the invention simplifies the extracting process, lowers the power consumption, reduces the pollution and has wide application prospect.

The invention relates to a non waste water producing method for yam saponin that includes the following steps: putting smashed raw materials into thermal decomposition device to take pressuring decomposing, controlling the temperature in 0-200degree centigrade and the pressure in 0.8-40MPa, and keeping for 5min-9h, drying the material and distilling in normal method. In the whole producing process, acid and alkali are not needed any more. It has no waste water, and has simple producing process, low cost, etc.

The invention discloses a composition taking cycloartenyl ferulate and 24-methylene cycloartenyl ferulate as active ingredients, a preparation of the composition used as a medicine, and an application of the preparation. The total content of the cycloartenyl ferulate and the 24-methylene cycloartenyl ferulate is over 90 percent of the total active ingredients by weight percentage. As the medicine, the composition can be injection and frozen-dried powder injection and also can be an oral solid preparation, a medicine composition used for treating autonomic imbalance, climacteric syndrome, primary dysmenorrheal and premenstrual tension, periodic psychosis, vascular headache, head trauma syndrome, gastrointestinal imbalance and hyperlipemia, etc.

The invention discloses a method for extracting phytosterol from waste residues generated in biodiesel production, comprising the following steps of: converting phytosterol in a combined state into free phytosterol through deep saponification; extracting the phytosterol through an organic solvent to prepare mother liquor of crude sterine; recovering the solvent to obtain a crude sterine product, wherein the extraction rate of the crude sterine can reach 88.6 percent; recrystallizing the crude sterine product through absolute ethyl alcohol to obtain high-purity phytosterol, wherein the purity of the obtained phytosterol can reach 91.3 percent, and the prepared phytosterol contains campesterol, stigmasterol and sitosterol. The invention has simple extraction and purification method, changes the produced waste residues of biodiesel into valuables and has a good industrial application prospect.

The invention provides a tripterygium diterpenoid lactone derivative shown in Formula II-6 or a pharmaceutically acceptable salt thereof, a preparation method thereof, a drug combination thereof and an application thereof in preparation of drugs for treatment of tumors, in particular to ovarian cancer or prostate cancer.