14 results about "Stigmasterol" patented technology

The invention relates to the technical field of cholesterol preparation, and discloses a preparation method of plant-derived cholesterol, which comprises the following steps: 1) heating and dissolving phytosterol with n-amyl alcohol, adding stigmasterol until saturation, cooling and crystallizing, and then carrying out solid-liquid separation to obtain a stigmasterol crude product; 2) heating and dissolving the stigmasterol crude product with absolute ethyl alcohol, adding pyridine, and continuously dropwise adding acetic anhydride for reaction; after the reaction is completed, adding deionized water for mixing, extracting with n-hexane for multiple times, separating an upper-layer oil phase, and concentrating and drying the oil phase to obtain a white solid; 3, the white solid is subjected to oxidation reduction, witting reaction and hydrogenation reaction, and cholesterol is obtained.The method has the advantages that compared with the prior art that the plant source cholesterol is directly prepared from the raw material phytosterol, the process is simplified, and meanwhile the product yield can be increased.

The invention provides a pharmaceutical composition for treating prostatitis and a preparation method thereof. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 20 to 60 parts of palmatine, 20 to 60 parts of paeoniflorin, 10 to 30 parts of formononetin, 20 to 60 parts of astilbin and 10 to 40 parts of stigmasterol. In-vitro cell tests and in-vivo animal experiments prove that the pharmaceutical composition has the effect of relieving the chronic prostatitis reaction.

The application provides a liquid chromatography-mass spectrometry method for detecting phytosterol content in dried blood spots, and belongs to the technical field of detection.The application simplifies a sampling process, uses a disposable blood taking needle and a blood taking card for sampling, replaces traditional serum or plasma samples with obtained dried blood spots, punches a sampling area of the dried blood spot sample through a puncher with a diameter of 3.2 mm, takes three dried blood spot samples, extracts seven kinds of sterol substances contained in the dried blood spot samples through n-hexane, adopts liquid chromatography-mass spectrometry, carries out detection and quantification through chromatographic separation and a mass spectrometry detector, realizes rapid and accurate determination of cholesterols, beta-sitosterols, daidzein, campesterol, dihydrocholesterol, dehydrocholesterol and 7-alkenyl cholestanol, and the method is simple in operation, accurate in quantification, and has a recovery rate of 92.4%-96.7%.

The invention relates to the technical field of stigmasterol monomer separation, in particular to a method for efficiently separating a stigmasterol monomer by using an amphiphilic glucoside derivative, which comprises the following steps: S1, capturing stigmasterol, S2, separating out a stigmasterol-glucoside compound, S3, obtaining a stigmasterol crude product, and S4, refining stigmasterol. The amphiphilic glycoside derivative is utilized to realize double recognition of a beta-CD cavity and a thioester bond, the stigmasterol binding amount is obviously higher than that of beta-sitosterol, the separation factor reaches 3.8 or above, and the separation effect is better.

The invention discloses a preparation method of a soybean seed coat polysaccharide encapsulated essential oil liposome antibacterial agent, which comprises the following steps: uniformly dispersing soybean lecithin (20mg / ml), stigmasterol (4mg / ml) and essential oil (1%) in absolute ethyl alcohol, carrying out rotary evaporation at 45 DEG C until a uniform lipid film is formed in a bottle, transferring into a vacuum drying oven, and further drying and removing ethyl alcohol; the method comprises the following steps: carrying out ultrasonic treatment on a liposome sample, hydrating by using a phosphate buffer solution (PBS, 0.01 mol / l, pH of 7.2-7.4) containing Tween-80, and carrying out ultrasonic treatment for 10 minutes under the power of 200W to obtain the liposome sample; uniformly mixing the soybean seed coat polysaccharide solution (0.01%-0.2%) with the liposome solution according to the volume ratio of 1: 1, and stirring for 1 hour to obtain a soybean seed coat polysaccharide encapsulated liposome solution; the slow-release antibacterial agent prepared by the preparation method disclosed by the invention has excellent slow-release effect and antibacterial property, and the preparation method is simple to operate and low in cost.

The invention relates to the field of synthesis of organic fluorescent dyes, and discloses a conjugate of indocyanine green and cholesterol as well as synthesis and application of the conjugate. The conjugate is formed by connecting an indocyanine green derivative and a cholesterol derivative through an amido bond; the indocyanine green derivative is ICG-R-COOH; iCG is indocyanine green, R is-(CH2) nH. [I-], n is an integer of 1-6, or R is-(CH2) mSO3-, and m is 3 or 4; and the cholesterol derivative is cholesterole-NH2, beta-sitosterole-NH2, stigmasterole-NH2 or ergosterole-NH2. The invention also discloses a preparation method of the cholesterol derivative. The lipidosome constructed by the conjugate is high in stability, good in dispersity and strong in NIR-II fluorescence, the blood vessel and tumor imaging contrast ratio and definition under a 1300 nm near-infrared imaging window are superior to those of lipidosome constructed by commercial ICG, more excellent diagnosis and treatment performance is shown, and wide application prospects are achieved.

The application relates to the technical field of natural medicines and liver damage treatment, in particular to application of stigmasterol in preparation of medicines for preventing and treating liver damage caused by APAP+ETOH, the medicines being medicines for target activation of AKT regulation of hepatocyte apoptosis, reversal of liver damage and inflammation, and improvement of liver index and liver function. The stigmasterol in the application can effectively reduce liver damage caused by APAP+ETOH at a certain dose, relieve pathological damage caused by hepatocyte necrosis and inflammatory cell infiltration induced by APAP+ETOH, and improve liver index and liver function. The raw material of the application is from natural product ginger, and the active ingredient is convenient to collect and has high safety.

The invention discloses a liver organoid culture medium and a culture method thereof, a liver organoid and application, and belongs to the technical field of biological medicine. The liver organoid culture medium is a basal culture medium containing stigmasterol, fetal calf serum, a B27 supplement, N-acetylcysteine, nicotinamide, recombinant human leucine 15-gastrin I, recombinant mouse epidermal growth factors, recombinant human fibroblast growth factors, A83-01, DAPT (Diamantadine Phosphate), dexamethasone and double antibodies PS (Poly Styrene). The stigmasterol is added, so that the growth and amplification of liver organs can be promoted. By applying the liver organoid culture medium, a simple, universal and efficient liver organoid culture method is provided, and the core bottleneck that amplification is difficult in liver organoid culture is solved.

The invention provides a preparation method of a stigmasterol surface molecularly imprinted polymer, which comprises the following steps: firstly, activating and modifying SiO2 with hydrochloric acid, then mixing methacrylic acid and methyl methacrylate to serve as a functional monomer, forming a stable compound with template molecule stigmasterol, adding the modified SiO2 into the compound, adding azodiisobutyronitrile, and reacting to obtain the stigmasterol surface molecularly imprinted polymer. Stirring in nitrogen, filtering, washing and drying to obtain the stigmasterol surface molecularly imprinted polymer. The stigmasterol surface molecularly imprinted polymer has specific adsorption capacity on stigmasterol, stigmasterol monomers can be specifically adsorbed from phytosterol, the stigmasterol adsorption capacity is 29.87 mg / g, and the purity reaches 97.23%. By optimizing the adsorption conditions, the adsorption capacity can be 114.71 mg / g, and the purity can be 97.41%.

The invention discloses a glycoside sterol component lipid nanoparticle for regulating and controlling mRNA transfection as well as a preparation method and application of the glycoside sterol component lipid nanoparticle, and belongs to the technical field of pharmaceutical preparation preparation. The nanoparticle is prepared from DLin-MC3-DMA, DOPE, different glycosides or sterols and DSPE-PEG-GSH. The different glycosides or sterols comprise amygdalin Amy, beta-sitosterol, stigmasterol Sti, ginsenoside Rg1 and salidroside Sal. Stigmasterol can improve enrichment of LNP in the spleen, ginsenoside can adjust immunity and enhance the anti-tumor immune effect, the preparation method is simple, needed equipment is conventional equipment, large-scale production can be achieved, and therefore the stigmasterol and ginsenoside compound has good industrial application value and market prospects.

The present disclosure relates to lipid emulsions for parenteral administration, comprising 10 to 30% of a lipid phase comprising soybean oil, medium-chain triglycerides (MCT), olive oil and fish oil and less than 25 mg, preferably less than 20 mg, campesterol, less than 30 mg, preferably less than 25 mg, stigmasterol and less than 120 mg, preferably less than 100 mg, betasitosterol per 100 g of the lipid phase. The present disclosure also relates to the lipid emulsions according to the present disclosure for providing parenteral nutrition, in particular to neonates, as well as to methods for manufacturing the lipid emulsions according to the present disclosure.

The present application belongs to the technical field of feed additive, and particularly relates to a compound fatty acid preparation for promoting chicken growth, and a preparation method and application thereof. The compound fatty acid preparation for promoting chicken growth is mainly composed of the following raw materials in parts by weight: 3-6 parts of stigmasterol, 2-4 parts of campesterol, 10-20 parts of linoleic acid, 12-24 parts of tributyrin, 1-2 parts of emulsifier, 3-5 parts of soybean phospholipid, 3-5 parts of protective agent, and 30-80 parts of water. The combination of stigmasterol and campesterol can significantly improve the targeted release efficiency of linoleic acid and tributyrin. The released butyric acid can activate the mitochondrial beta-oxidation pathway of intestinal epithelial cells, significantly improve the efficiency of tricarboxylic acid cycle, and enhance the intestinal barrier function. The phytosterol can also competitively inhibit the NPC1L1 transporter, reduce the intestinal cholesterol absorption, direct the energy to the growth demand, thereby improving the daily weight gain of broilers and reducing the feed-meat ratio.

The invention discloses a fertilizer composition for promoting yield and income increase of tomatoes and a preparation method, and belongs to the technical field of fertilizers. The fertilizer composition is prepared from the following components in parts by mass: a major element compound, a medium element compound, a bioactive master batch, a chelated trace element compound, diethyl aminoethyl hexanoate, compound sodium nitrophenolate, chitosan oligosaccharide, stigmasterol, jasmonic acid methyl ester and brassinolide, the bioactive master batch is prepared from soybean deodorized distillate fermentation liquor, humic acid powder and calcium carbonate, wherein the soybean deodorized distillate fermentation liquor is liquid obtained by fermenting a soybean deodorized distillate extract through candida tropicalis and bacillus subtilis; the prepared fertilizer composition not only has innovativeness in component composition, but also realizes breakthrough in action mechanism and application effect, and provides an effective solution for breaking the technical bottleneck that yield increase and stress resistance are difficult to cooperate in current tomato production.

This invention discloses the preparation and application of a recombinant cholesterol esterase immobilized enzyme preparation. This invention will... Ophiostoma piceae Cholesterol esterase mutant gene ope-D136P Linked to pPICZαA plasmid, via signal peptide replacement and rDNA Gene insertion yielded the pEESB-3 plasmid; the protein disulfide isomerase gene was inserted. pdi The pPIC9K plasmid was ligated to obtain the pEESB-4 plasmid. This plasmid was electroporated into Pichia pastoris GS115, and after resistance selection and methanol-induced expression selection, recombinant Pichia pastoris engineered strains were obtained. Recombinant cholesterol esterase was obtained from this strain after methanol-induced expression. An aminated immobilization vector was prepared using APTES and TEOS as silane precursors. After grafting with 2,4-dihydroxyacetophenone and nickel ion chelation, the recombinant cholesterol esterase was immobilized to obtain an immobilized enzyme preparation. This immobilized enzyme preparation can catalyze the synthesis of stigmasterol ferulic acid ester from stigmasterol in a non-aqueous phase system, with a conversion rate of 33% (based on stigmasterol) or 32% (based on ferulic acid).