84 results about "Enrofloxacin" patented technology

The invention discloses a nucleic acid aptamer of enrofloxacin and ciprofloxacin, an enzyme sensor and application thereof, and belongs to the technical field of nucleic acid aptamers. The nucleotide sequence of the nucleic acid aptamer for the enrofloxacin and the ciprofloxacin is shown as SEQ ID NO.3. The invention also discloses application of the nucleic acid aptamer for the enrofloxacin and the ciprofloxacin in detection of the enrofloxacin and the ciprofloxacin. The enzyme sensor comprises the following components: nucleic acid aptamers of enrofloxacin and ciprofloxacin, an auxiliary chain H1, an auxiliary chain H2, an auxiliary chain H3, copper sulfate and a TMB (Tetramethylbenzidine) developing solution. The enzyme sensor is applied to detection of enrofloxacin and ciprofloxacin. The nucleic acid aptamer of the enrofloxacin and the ciprofloxacin is obtained through optimization and transformation and has higher affinity to a target, the Kd value of the nucleic acid aptamer to the enrofloxacin is 15 nM, and the Kd value of the nucleic acid aptamer to the CIP is 123 nM. The enzyme sensor disclosed by the invention can realize specific recognition of ENR and CIP, is high in sensitivity and has a wide application prospect.

The present invention belongs to the technical field of biological treatment of environmental pollutants, and specifically relates to a strain of low-temperature-resistant multifunctional bacteria and its application in soil and water pollution remediation. The low-temperature-resistant multifunctional bacteria is the strain TLT1 of the genus Tularemia, which was deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration on August 9, 2023, with the deposit number: CGMCC No: 40754. The bacterial agent of the present invention can be used to stabilize zinc in the soil environment, which helps to enrich the database of bacterial strains that stabilize heavy metals and have the ability to degrade quinolone antibiotics. The present invention prepares the purified strain into a bacterial suspension, which can effectively stabilize zinc in different environmental media such as water and soil under low-temperature conditions, and has the ability to degrade enrofloxacin in different environmental media such as water and soil. Compared with methods such as chemical oxidation, this method is more efficient, less expensive, and has no secondary pollution, providing an effective biological-non-biological combined remediation technology for the remediation of zinc-enrofloxacin complex-contaminated soil and water.

The invention provides a wide-spectrum dual-photoelectrode-based photoelectrochemical sensor and a preparation method and application thereof, and belongs to the technical field of photoelectrochemical sensors. According to the photoelectrochemical sensor provided by the invention, a BCN / ITO electrode is used as a photoanode, a CuInS2 / ITO electrode is used as a photocathode, BCN is Bi2S3 / C3N4, and ITO is indium tin oxide. The sensor is used for detecting enrofloxacin and ciprofloxacin pollution. According to the present invention, the surface is modified with the aptamer having the specific recognition function, the specific binding effect of the aptamer with the enrofloxacin and the ciprofloxacin is utilized to form the complex of the aptamer and the target object, and the influence of the caused steric hindrance effect on the photoelectric signal and the dual-channel strategy are utilized to achieve the simultaneous detection of the residual marker; the linear ranges for detecting enrofloxacin and ciprofloxacin are 0.1 nM to 1 mu M and 50 pM to 0.5 mu M respectively, the detection limits are 24 pM and 15.5 pM respectively, and the method is successfully applied to simultaneous detection of residual markers in water. The method has the advantages of greenness, environmental protection, wide detection range, high sensitivity, good selectivity and high stability.

This invention provides a composite aerogel based on Eucommia ulmoides gum and MXene nanosheets, its preparation method, and its application. The aerogel is prepared by reacting a toluene solution of Eucommia ulmoides gum with Ti3C2T... x MXene aqueous solution was mixed and a water-in-oil microemulsion was formed using a combination of ultrasound-assisted shaking. This allowed MXene nanosheets to self-assemble at the microemulsion droplet interface. Following vertical freezing and freeze-drying processes, a three-dimensional network structure of Eucommia ulmoides gum-Ti3C2T was successfully constructed. x MXene composite aerogel. The preparation method of this invention is mild and requires no chemical cross-linking agents. The resulting composite aerogel exhibits excellent adsorption performance for enrofloxacin, malachite green, Congo red, rhodamine, and kanamycin in water, with adsorption equilibrium times all within 10 minutes, enabling rapid removal of organic pollutants from water. This invention provides a new approach for the high-value utilization of Eucommia ulmoides gum and has promising application prospects in the field of water environment remediation.

A fermented traditional Chinese medicine feed additive for catfish, its preparation method, and its application are disclosed, relating to the field of aquatic feed additives. The preparation method involves activating and expanding the culture of *Saccharomyces cerevisiae*, *Bacillus subtilis*, and *Lactobacillus plantarum*; pulverizing *Paeonia suffruticosa* leaves, *Smilax china*, *Scutellaria baicalensis*, *Rhus chinensis*, hawthorn, *Polygonum cuspidatum*, ginkgo, and forsythia suspensa* to obtain traditional Chinese medicine powders; preparing a solid fermentation medium using the traditional Chinese medicine powders, soybean meal powder, corn cob granules, and wheat bran; inoculating *Saccharomyces cerevisiae* seed culture and *Bacillus subtilis* seed culture for aerobic fermentation; and inoculating *Lactobacillus plantarum* seed culture for anaerobic fermentation to obtain the fermented traditional Chinese medicine feed additive. The resulting fermented traditional Chinese medicine feed additive promotes the absorption and metabolic elimination of enrofloxacin in catfish, shortens the withdrawal period, has preventive and adjunctive therapeutic effects on bacterial diseases in catfish, promotes growth, reduces feed conversion ratio, and improves survival rate.

The present invention belongs to the technical field of veterinary preparations, and specifically relates to a veterinary enrofloxacin compound gel ear drops and its preparation method and application. The compound gel ear drops include four active substances: enrofloxacin, triamcinolone acetonide, gentamicin sulfate, and pulegone. The preparation method is to add poloxamer, L-cysteine, and carbomer to water, stir and add benzyl alcohol to obtain solution A, dissolve low-erucic acid rapeseed oil in ethanol of more than 85%, add enrofloxacin, triamcinolone acetonide, pulegone, and gentamicin sulfate to obtain solution B; slowly add solution B to the gel matrix of solution A to obtain solution C, and then filter to obtain the compound gel. Pharmacodynamic experiments have confirmed that it has a good therapeutic effect on inflammation of the external ear canal of pets; accelerated experiments have confirmed that the compound gel is stable in nature, can be stored for a long time, and is suitable for further expansion of production.

The invention discloses a method for ratio-dependent fluorescence detection of enrofloxacin based on a new composite nano carbon dot-gold cluster material and application, and belongs to the technical field of advanced non-ferrous metal nano new materials and new material detection. The new composite nano carbon dot-gold cluster material AuNCs-CDs can be prepared by mixing gold nano cluster seeds and ascorbic acid according to a certain proportion and carrying out microwave heating; under the same excitation wavelength, the AuNCs coated CDs have two emission peaks of 440 nm and 640 nm. The method comprises the following steps: adding enrofloxacin with different concentrations into an AuNCs (at) CDs solution, and measuring the fluorescence intensity ratio I440 / I640 of the AuNCs (at) CDs; and drawing a standard curve according to the relationship between the concentration of the enrofloxacin solution and the fluorescence intensity ratio of the AuNCs coated CDs solution, so as to detect the concentration of the enrofloxacin. In addition, the method can be applied to experimental recovery rate detection of enrofloxacin in an actual sample. The method disclosed by the invention has high sensitivity and selectivity, is simple and rapid, does not need any pretreatment, and shows an extremely wide application prospect in many fields such as biomedical detection, environmental monitoring, food safety analysis and the like.

The present application belongs to the technical field of wastewater treatment, and particularly relates to a seawater breeding wastewater antibiotic removal method based on oyster shell composite filler. Fine gravel, oyster shell and functional mineral are compounded in a fixed volume ratio of 2:1:1, and are laid in a structure with a limited particle size range and homogeneity without stratification. A salinity-directed salt-tolerant domestication process and a tidal intermittent operation mode of 48h hydraulic retention + 24h emptying and reoxygenation are matched, and salt-tolerant functional bacterial flora of Proteobacteria, Paracoccus and Shigella are enriched. The present application can adapt to a wide range of salinity conditions of 10‰-30‰, relieve high salt inhibition and ion competition, simultaneously and efficiently remove three typical antibiotics of conventional pollutants, sulfamethoxazole, trimethoprim and enrofloxacin, and reduce resistance genes. The sulfamethoxazole removal rate can reach 93.94% under the condition of 30‰ high salinity, the system runs stably and has strong adaptability, and has certain engineering application value and large-scale popularization potential in the field of seawater breeding wastewater treatment.

The application discloses a small-molecule multi-connection detection kit based on a biochip, a preparation method and a detection method thereof. The kit comprises nano-gold particle labeled antibodies, a multi-connection detection chip plate and a detection buffer. The nano-gold particle labeled antibodies comprise nano-gold particle labeled florfenicol antibodies and enrofloxacin antibodies. The multi-connection detection chip plate is coated with florfenicol antigens and enrofloxacin antigens in detection holes. The detection buffer is composed of 10-40 mM phosphate buffer and 0.05-0.5 wt% surfactant S17. The application can simultaneously detect florfenicol and enrofloxacin small molecules by using one detection buffer, thereby saving time and labor.

The present invention relates to the technical field of antioxidant technology for livestock sperm, and provides a highly efficient antioxidant donkey sperm diluent, a preparation method thereof, and an application. A highly efficient antioxidant donkey sperm diluent includes a basal medium and additives, wherein: the basal medium contains the following components by weight in every 1000 mL of water: 30 - 60 g of glucose, 4 - 6 g of 4 - (2 - hydroxyethyl)piperazine - 1 - ethanesulfonic acid, 12 - 24 g of skim milk powder, and 0.5 - 1.1 g of potassium citrate; the additives contain the following components by weight in every 1000 mL of the basal medium: 5 mg - 10 mg of sulforaphane, 0.1 mg - 0.2 mg of stigmasterol, 40 μg - 100 μg of enrofloxacin concentrate, and 3 mg - 5 mg of cefotaxime sodium. The present invention can effectively improve the conception rate of female donkeys, reduce the incidence of metritis, and improve the in vitro sperm motility.

The application discloses a kind of enrofloxacin rapid detection methods based on fluorescent covalent organic framework magnetic beads, belong to food safety detection field.The method uses the fluorescent covalent organic framework magnetic bead of coupling enrofloxacin monoclonal antibody, realizes detection in combination with immunochromatography test strip.Fluorescent covalent organic framework magnetic bead realizes target enrichment separation with inner core, shell fluorescent covalent organic framework material high-efficiency coupling antibody and exports fluorescence signal;Test strip is equipped with detection line (coated enrofloxacin artificial antigen) and quality control line (coated secondary antibody).By comparing the fluorescence intensity of detection line and quality control line, trace enrofloxacin in complex matrix can be qualitatively and quantitatively analyzed.The method is simple, rapid and sensitive, without large instrument, can be detected on site.

The invention discloses a light-emitting probe based on a europium-based metal-organic framework and a preparation method and application thereof, and belongs to the technical field of fluorescent sensing materials and food safety detection.The light-emitting probe is a europium-based metal-organic framework material, the chemical formula of the light-emitting probe is Eu (phen) (5-hipa) NO3 <->, phen is 1, 10-phenanthroline ligand, 5-hipa is 5-hydroxyisophthalic acid ligand, and the molecular weight of the Eu (phen) (5-hipa) NO3 <-> is larger than that of Eu (phen) (5-hipa) NO3 <->. The light-emitting probe takes a binuclear Eu cluster as a metal center. According to the luminous probe based on the europium-based metal-organic framework, the synthesized europium-based metal-organic framework material can be used as a bifunctional fluorescent probe, and specific recognition and quantitative detection of carbaryl and enrofloxacin are realized in an aqueous solution; the method has the remarkable effects of high sensitivity, strong anti-interference capability, recyclability and simplicity, convenience and rapidness in operation.

The invention discloses construction of a self-energized sensor based on HOF-on-MOF and application research of enrofloxacin in mandarin fish, and belongs to the field of self-energized sensing, a photocathode material and a photoanode material required by the self-energized sensor are prepared by applying a hydrothermal synthesis method, the self-energized sensor is constructed by utilizing the photocathode material, and the self-energized sensor is used for detecting enrofloxacin in mandarin fish. An aptamer (Apt) is successfully combined to a photocathode, the self-powered photoelectrochemical aptamer sensor is constructed, the photocurrent value of the self-powered sensor is linearly related to the logarithm of the concentration of enrofloxacin (ENR), the photocurrent value after the ENR is added is substituted into a linear regression equation of a standard curve drawn by the photocurrent and the logarithm of the concentration, and the self-powered photoelectrochemical aptamer sensor is constructed. Compared with an existing ENR detection method, the method has the advantages that the detection range is wider (1.0 * 10 <-11 >-1.0 * 10 <-6 > mol / L) and the detection limit is lower (3.3 * 10 <-12 > mol / L), and trace detection of the ENR can be realized.

The application discloses a compound enrofloxacin injection, which contains 10-25g of enrofloxacin, 2-10g of sulfadimoxine sodium, 5-12g of florfenicol, 0.5-2.5g of bromhexine hydrochloride, 1-20g of glacial acetic acid, 2-5g of Tween 80, 5-15g of alpha-pyrrolidone and 5-20g of N, N-dimethylformamide per 100ml of the injection, and the rest is water for injection. The compound enrofloxacin injection has advanced and reasonable prescription design, stable and reliable preparation process, good preparation stability and small irritation, and can achieve the effect of treating all samples for respiratory system diseases of livestock and poultry.

The application belongs to the field of electrochemical detection, and particularly relates to a novel water-vapor coupling photoelectric self-powered electrochemical sensor for detecting enrofloxacin and a preparation method thereof. A glass substrate is used as a substrate, a copper foil tape is used as a positive electrode and a negative electrode, CdS (cadmium sulfide) and a cobalt-based metal organic framework (ZIF-67) are used as water-vapor / photovoltaic materials to construct a heterojunction for preparing a water-vapor generator to realize sustainable power generation. The CdS / ZIF-67 heterojunction is beneficial to the separation of photo-generated carriers under illumination and synergistically enhances the water evaporation effect. An enrofloxacin (ENR) aptamer is assembled to construct a water-vapor / photovoltaic self-powered electrochemical sensor. With the help of split aptamers, the ENR detection exhibits high sensitivity and high specificity, and provides a new strategy for self-powered electrochemical detection. The water-vapor / photovoltaic self-powered electrochemical sensor has a wide detection range, high sensitivity and low detection limit.

The invention provides a veterinary enrofloxacin lipid nanoemulsion and a preparation method and application thereof, and the veterinary enrofloxacin lipid nanoemulsion comprises the following components by mass: 5% of enrofloxacin, 5-10% of EL40, 5-10% of soybean lecithin, 0.1-0.3% of CMC-Na, 0.1-0.5% of saccharin sodium, 0.1-0.5% of Arabic gum, and the balance of sterile water. The particle size is 140-150 nm, the PDI is less than or equal to 0.35, and the Zeta potential is-30 to-50 mV; the encapsulation efficiency is greater than or equal to 99.5%, and the drug loading capacity is greater than or equal to The encapsulation efficiency and the drug loading capacity are high, the drug effect maximization is ensured, the encapsulation efficiency is larger than or equal to 99.5%, the drug loading capacity is larger than or equal to 4.8%, the drug is almost completely wrapped by the lipid carrier, the free drug loss is reduced, and the bioavailability is remarkably improved; the high encapsulation efficiency can reduce the degradation of the drug in the gastrointestinal tract and improve the concentration of the target tissue drug.

The application provides enrofloxacin suspension and a preparation method and application thereof, and is characterized in that, according to 100% by weight, the enrofloxacin suspension comprises the following components: enrofloxacin 4%-6%; sodium carboxymethyl cellulose 0.4%-0.6%; hydroxyphenyl ethyl ester 0.04%-0.06%; and purified water is supplemented to 100%. The drug particle size D90 in the enrofloxacin suspension is 2-10 microns. By limiting the weight ratio of each component and the synergistic effect between the formula, and by matching the drug particle size range of the suspension, the following comprehensive technical effects are achieved: long-acting slow release, low irritation, applicability to pregnant animals, multi-pathway drug delivery, low sedimentation rate, and almost no stratification.

The application discloses an anti-ENR nanobody, a coding gene, a cELISA detection method and purposes, and belongs to the technical field of biology. The amino acid sequence of the anti-ENR nanobody is shown as SEQ ID NO. 1, and the nucleic acid sequence for coding the anti-ENR nanobody is shown as SEQ ID NO. 2. A new type of competitive ELISA (cELISA) is established by screening the specific nanobody against ENR from an immunized bactrian camel library. When detecting the enrofloxacin residue in animal-derived food, the method has high sensitivity and specificity, can detect the ENR residue as low as 6.49 ng / mL in the sample, and has no cross reaction with other antibiotics. It is shown that the cELISA established in the application is a simple, rapid, sensitive and low-cost immunodetection method, and has great application prospect in the detection of ENR residue in animal-derived food.

The spray drying device comprises a bottom plate, the middle side of the upper end of the bottom plate is connected with a machine body, the lower side of the interior of the machine body is connected with a hot air pipe, and the lower side of the machine body is connected with a discharging pipe; an atomizer is arranged on the middle side of the upper end of the machine body, a stirring structure is connected to the middle side in the machine body, and the stirring structure comprises a connecting plate, a motor and a connecting wheel; and the connecting pipe is mounted on the outer side of the hot air pipe, the upper side of the connecting pipe is connected with a mounting plate, the outer side of the upper end of the mounting plate is connected with a flow guide plate, a scraping structure is mounted on the outer side of the mounting plate, and the scraping structure comprises a scraping plate and a fixing plate. According to the spray drying device for preparing the enrofloxacin water emulsion particles, the stirring structure is arranged, mass transfer can be promoted, the material uniformity can be guaranteed, and therefore the drying effect of enrofloxacin water emulsion is improved, and the scraping structure is arranged, so that the inner wall of the drying device can be cleaned.

The invention belongs to the technical field of auxiliary materials for veterinary drugs, and particularly relates to a water-soluble auxiliary material for veterinary drugs as well as a preparation method and application thereof. The water-soluble auxiliary material for the veterinary drug comprises the following components in parts by mass: 70-80 parts of magnesium sulfate, 30-40 parts of fermented cassava starch, 0.1-1 part of polyvinylpyrrolidone, 0.5-3 parts of a surfactant, 1-2 parts of a pH regulator and 0.1-1 part of a sweetening agent. The fermented cassava starch is prepared by fermenting cassava native starch prepared from fresh cassava through compound bacteria, and the compound bacteria comprise bacillus velezensis, bacillus subtilis and bacillus amyloliquefaciens. The water-soluble veterinary drug auxiliary material prepared by the invention can improve the dissolution rate and storage stability of enrofloxacin.

The application belongs to the technical field of veterinary drug excipients, and particularly relates to a water-soluble veterinary drug excipient and a preparation method and application thereof. The water-soluble veterinary drug excipient comprises the following components in parts by mass: 70-80 parts of magnesium sulfate, 30-40 parts of fermented cassava starch, 0.1-1 part of polyvinylpyrrolidone, 0.5-3 parts of a surfactant, 1-2 parts of a pH regulator, and 0.1-1 part of a sweetening agent. The fermented cassava starch is prepared by fermenting cassava raw starch prepared from fresh cassava by using composite bacteria, and the composite bacteria comprise Bacillus velezensis, Bacillus subtilis and Bacillus amyloliquefaciens. The water-soluble veterinary drug excipient prepared by the application can improve the dissolution rate and storage stability of enrofloxacin.

The invention provides a method for evaluating an activated sludge system response mechanism under antibiotic impact and application thereof, and belongs to the technical field of water environment risk evaluation and management. The method mainly relates to detection of water quality indexes (ammonia nitrogen, total nitrogen, total phosphorus and chemical oxygen demand) in the activated sludge and physicochemical characteristics (extracellular polymeric substance EPS, sludge settling performance and Zeta potential) of the activated sludge, and a response mechanism of an activated sludge system under antibiotic impact is analyzed by taking ciprofloxacin (CIP), ofloxacin (OFL) and enrofloxacin (ENR) as indication pollutants. Effective reference is provided for system regulation and control when a sewage plant faces antibiotic impact, and a new insight is provided for removing antibiotics and drug-resistant genes in wastewater by explaining the relation among functional genes, drug-resistant genes and microbial communities in an activated sludge system under antibiotic exposure.

The invention provides application of epiberberine, palmatine hydrochloride and a composition of epiberberine and palmatine hydrochloride as an antibacterial synergist. The method comprises the following steps: firstly, respectively researching in-vitro antibacterial effects of epiberberine and palmatine hydrochloride on Escherichia coli; then the in-vitro antibacterial effect of the epiberberine and palmatine hydrochloride combined antibacterial drug (enrofloxacin and doxycycline hydrochloride) on the escherichia coli is researched respectively, and then the in-vitro antibacterial effect of the epiberberine and palmatine hydrochloride combined antibacterial drug (enrofloxacin and doxycycline hydrochloride) on the escherichia coli is researched.

The application provides a composite material with adsorption-photocatalysis synergistic effect and a preparation method and application thereof, relates to the technical field of materials, and comprises the following steps: mixing ethylene glycol and bismuth nitrate to form a solution, slowly adding a sodium molybdate and sodium tungstate aqueous solution, then adding titanium dioxide, mixing, and then carrying out constant-temperature reaction in a high-pressure reaction kettle; and then sequentially carrying out suction filtration, washing, and vacuum drying to obtain Bi2W x Mo 1‑x O6 / TiO2. x Mo 1‑x O6 / TiO2 is added into a dispersion solution of graphene oxide, ultrasonic reaction is carried out, urea is added, and continuous ultrasonic reaction is carried out; the obtained mixed solution is moved into a high-pressure reaction kettle for constant-temperature reaction; after the reaction is completed, sequential suction filtration, washing, and vacuum drying are carried out to obtain Bi2W x Mo 1‑x O6 / TiO2 / rGO material. The application has the advantages of convenient preparation, low energy consumption, high yield, cleanness, environmental protection, high adsorption-photocatalysis synergistic removal activity of the obtained product to organic matters such as enrofloxacin and rhodamine B, excellent repeated use performance, and good application prospect in the field of organic wastewater purification treatment.

The invention discloses a porous carbon electrode based on gold nanoparticles / poly (3, 4)-ethylenedioxythiophene, a preparation method of the porous carbon electrode and application of the porous carbon electrode in antibiotic detection, and belongs to the technical field of food. The preparation method based on the gold nanoparticle / poly (3, 4)-ethylenedioxythiophene / porous carbon electrode comprises the following steps: (1) engraving the porous carbon electrode by using a laser engraving machine; (2) putting the carbon electrode into a (3, 4)-ethylenedioxythiophene solution, and polymerizing (3, 4)-ethylenedioxythiophene on the porous carbon electrode by utilizing a potentiostatic method; (3) placing the electrode prepared in the step (2) in a chloroauric acid solution, and depositing gold nanoparticles on the electrode through cyclic voltammetry; the gold nanoparticle / poly (3, 4)-ethylenedioxythiophene / porous carbon electrode provided by the invention can sensitively detect chloramphenicol and enrofloxacin at the same time, and can be used for detecting chloramphenicol and enrofloxacin in tap water.

The application discloses a method for rapidly removing enrofloxacin in a breeding tail water treatment technology "three pools and two dams" by micro-nano aeration in an aeration tank in cooperation with calcium peroxide, and specifically comprises the following steps: installing a micro-nano bubble generating device, placing the water inlet end of a water inlet pipe and the water outlet end of a water outlet pipe of the micro-nano bubble generating device below the liquid level in the aeration tank, and starting the micro-nano bubble generating device; spraying calcium peroxide; after the aeration tank stably generates micro-nano bubbles, spraying calcium peroxide in the aeration tank to degrade enrofloxacin in the breeding tail water in the aeration tank at a concentration of μg / L level. The breeding tail water is circulated through the micro-nano bubble generating device, a large number of micro-nano bubbles are generated, and the oxygen-producing agent calcium peroxide which is frequently used in breeding management activities is added. The calcium peroxide is non-toxic, can release oxygen when meeting water, can rapidly generate high-activity oxygen in the water body, can increase the dissolved oxygen content of the water body, can generate a large number of free radicals, can improve the oxygen mass transfer efficiency, and can rapidly and effectively remove enrofloxacin in the breeding tail water.

The invention discloses a vibrio parahaemolyticus hfq gene deleted strain as well as a construction method and application thereof. The invention provides a deletion strain, a construction method of the deletion strain and application of the deletion strain in construction of an enrofloxacin drug resistance model. The invention finds that after the hfq gene is deleted, the drug resistance to the enrofloxacin is obviously increased. It is further proved that the vibrio parahaemolyticus ATCC33846 delta hfq deletion strain enhances the drug resistance to enrofloxacin by improving the excretion of rhodamine 6G. The vibrio parahaemolyticus deletion strain is constructed by utilizing high efficiency and accuracy of homologous recombination, and the important effect of the hfq gene on the drug resistance of the vibrio parahaemolyticus is proved by comparing the drug resistance of the deletion strain and a wild strain on enrofloxacin. The compound can be used for developing a new therapy for drug-resistant bacteria or improving an existing antibacterial strategy.

The invention belongs to the technical field of biochar preparation, and discloses potassium carbonate modified enteromorpha biochar as well as a preparation method and application thereof. N and S element doped potassium carbonate modified enteromorpha biochar with high specific surface area is prepared through two-step heating, activation and pyrolysis, so that the biochar is promoted to form a rich pore structure, and the adsorption capacity is improved. The modified biochar prepared by the method is large in specific surface area and rich and uniform in pores, has the adsorption capacity remarkably superior to that of a conventional carbon material, can be applied to actual wastewater to remove antibiotic pollutants, can well adsorb and remove enrofloxacin, is beneficial to ecological environment protection, and has relatively large economic benefits, social benefits and environmental benefits.

The present invention discloses a method for processing and detecting a sample. The present invention is based on the addition of a phase separator, a purifier and an extractant to respectively realize extraction and oil removal treatment, solves the difficulty of large conflicts in the extraction and oil removal process due to the large difference in the chemical properties of enrofloxacin, malachite green and diazepam, realizes a four-in-one unified sample pretreatment method of chloramphenicol, diazepam, malachite green and enrofloxacin, and the sample is pretreated once. Relative to the sample pretreatment method of a single compound, under the premise of ensuring high sensitivity, the detection time is greatly shortened, and it is subsequently applicable to the on-site rapid large-scale screening test of the colloidal gold method, and the minimum detection limit of each compound is 0.1μg / kg, 0.5μg / kg, 0.5μg / kg and 2μg / kg, respectively, which meets the accuracy and stability requirements of the detection and is convenient for the detection operation of grassroots personnel.

The application discloses a phosphorus-sulfur synergistic antibiotic bacterial residue biochar, a preparation method and application thereof, and belongs to the technical field of biochar. The application takes penicillin bacterial residue and high-sulfur wet-process phosphoric acid as raw materials, and prepares biochar through phosphorus-sulfur synergistic modification and applies the biochar to adsorption of antibiotic wastewater, so that efficient purification of the antibiotic wastewater and full-resource utilization of the bacterial residue are realized. The method innovatively uses cheap high-sulfur wet-process phosphoric acid to replace traditional strong acid (hydrochloric acid, sulfuric acid, etc.) for pretreatment, and the obtained acid hydrolysis solution can be directly used for preparing fertilizer, thereby simultaneously reducing pyrolysis cost and improving nitrogen utilization rate. The phosphorus-sulfur in-situ modification process makes the carbon yield of the biochar increase by 10%, the specific surface area increase by 45%, and the resistant genes in the bacterial residue are completely eliminated. The prepared biochar has an antibiotic removal rate of more than 99% for antibiotic wastewater such as enrofloxacin and tylosin, and the wastewater COD and ammonia nitrogen removal rates reach 96% and 71% respectively, so that the high-value utilization of antibiotic bacterial residue is realized while the environmental safety is ensured.