335 results about "Enrofloxacin" patented technology

The invention includes compositions for transmucosal administration to an animal comprising at least one active agent and a pharmaceutically acceptable carrier. A preferred active agent is selected from the group consisting of meloxicam, carprofen, enrofloxacin, clemastine, diphenhydramine, digoxin, levothyroxine, cyclosporine, ondansetron, lysine, zolpidem, propofol, nitenpyram, ivermectin, milbemycin, and pharmaceutically acceptable salts, solvates and esters thereof. In another embodiment, the invention includes methods of treating or preventing a condition in an animal comprising transmucosally administering a composition comprising a therapeutically or prophylactically effective amount of an active agent and a pharmaceutically acceptable carrier.

The invention discloses a fluorescent microsphere immunochromatography detection card for detecting enrofloxacin and a preparation method thereof. The detection card sequentially comprises filter paper, a sample pad, a glass fiber film, a nitrocellulose film and absorbent paper, wherein a fluorescent microsphere-labeled antibody is sprayed on the glass fiber film; a detection area and a quality control area are fixed on the nitrocellulose film; a conjugate of enrofloxacin and carrier protein is sprayed in the detection area; and an anti-mouse antibody is sprayed in the quality control area. By taking core-shell bistructure luminous nano particles complexed by silica and fluorescent substances as labels and adopting immunochromatography in a competition blocking mode, the invention realizes rapid immunoassay of the enrofloxacin. In the detection process, an optimized excitation light source of the fluorescent microsphere is adopted for excitation, the emitted fluorescence passes through a light filter device, and that whether a detection line is provided with fluorescent substances is observed by naked eyes. The invention has the characteristics that: the detection card has high sensitivity and is rapid in detection, convenient to operate, and economic and practical.

The invention relates to an enrofloxacin/sulfobutylether-beta-cyclodextrin inclusion compound, a preparation method and a medicinal preparation thereof. The inclusion compound comprises sulfobutylether-beta-cyclodextrin and enrofloxacin in a weight ratio of 1-10:1, and sulfobutylether-beta-cyclodextrin respectively has the average degree of substitution of 1, 4 and 7. The preparation method comprises the following steps: (1) weighing raw materials in a mass ratio of sulfobutylether-beta-cyclodextrin to enrofloxacin of 1-10:1, and respectively dissolving in a solvent which is water, ethanol solvent or mixture of water and ethanol solvent; and (2) mixing the two liquids prepared in the step (1) to perform an inclusion reaction. The medicinal preparation can be oral preparation and injection, wherein the oral preparation can be tablets, soluble powder and oral liquid; and the injection can be injection liquid and lyophilized powder. The medicinal preparation has the advantages of remarkably improved solubility in water phase, improved stability, excellent palatability, good curative effect and low toxic and side effects.

The invention discloses a solid phase extraction and HPLC-fluorescence detection method for fluoroquinolones antibiotics. The method comprises the following steps of passing a pretreated water sample through an activated solid phase extraction column; eluting the solid phase extraction column with a Na2EDTA aqueous solution; then vacuumizing; and finally, eluting the water sample for three times by a concentrated ammonia methanol solution with a concentrated ammonia volume percent concentration of 6 percent by 0.5-2mL each time. An obtained eluent is blow-dried to 0.1-0.4mL in air flow, water is added to reach a constant column of 1.0mL so as to obtain a test sample, and then HPLC-fluorescence detection is carried out. By adopting the solid phase extraction-HPLC-fluorescence detection technology and utilizing a conventional analytical instrument, the method can simultaneously measure the contents of four typical fluoroquinolones antibiotics of ofloxacin, norfloxacin, ciprofloxacin and enrofloxacin in the water sample. The method has simple operation and low cost and can be applied to the measurement of the ultratrace of four typical fluoroquinolones antibiotics in waste water and surface water.

The invention discloses a prescription composition of a pig antiviral semen diluent. Based on every 1000ml of double distilled water, the pig antiviral semen diluent contains 15.50g of glucose, 11.65g of trisodium citrate, 2.35g of EDTA sodium salt, 1.75g of sodium bicarbonate, 1.00g of polyvinyl alcohol, 5.50g of tricarboxymethyl amino methane, 4.10g of citric acid, 0.07g of cysteine, 1.00g of vitamin C, 0.10g of enrofloxacin and 50ml of an antiviral agent. According to the embodiment of the invention, the diluent of the prescription composition of the pig antiviral semen diluent has obvious killing, inhibition and obstructing effects on pig reproduction and respiration syndrome virus (PRRSV) and is capable of obviously improving the survival rate and preservation time of semen during in vitro preservation and the litter size of a mated sow.

The invention relates to the field of pharmaceutical preparations and particularly relates to a slow-release micropill preparation containing enrofloxacin and a preparation method of the preparation. The slow-release micropill provided by the invention is formed by coating an enrofloxacin micropill; the enrofloxacin micropill comprises enrofloxacin and auxiliary material and is formed through extruding and rounding; the auxiliary material is any one or more of microcrystalline cellulose, starch, cane sugar, artificial gum, lactose and sodium carboxymethyl starch; according to weight percent, the auxiliary material in the micropill accounts for 70% to 95%; and coating is made of high-molecular enteric material, film forming material, opaquer and the like. The slow-release micropill preparation has the characteristics of slow release and high bioavailability, can be used for treating bacteria and mycoplasma infection of livestocks, and has better curative effect on chronic respiratory diseases, colibacillosis and salmonellosis, and the frequency of medicine taking can be reduced; and in addition, the slow-release micropill provided by the invention has the advantages that the stability of medicine is improved, and peculiar bitter of enrofloxacin can be covered completely, so that feeding intake of the animals is not influenced, and the recovery rate is improved.

The invention discloses an enrofloxacin microcapsule preparation and a preparation method thereof. The enrofloxacin microcapsule preparation is prepared by 5 percent to 90 percent of enrofloxacin raw material and 95 to 10 percent of accessories, and the accessory is one of the followings or a mixture mixed by a plurality of the followings: 12 to 18 carbon fatty acid, calcium stearate monoglycerol ester, stearyl alcohol, saturated triglyceride, glycerol monoester, paraffin, animal wax, vegetable wax and fat powder, etc.. The appearance of the enrofloxacin microcapsule preparation is a white or light yellow spherical granule, the dispersion performance is good, the used accessory is safe, and the enrofloxacin microcapsule preparation has no toxicity or side effects for livestock and poultry and has a gradual release in stomach and intestine, thus maintaining the durability of the efficacy.

The invention especially relates to high-content weak acidic enrofloxacin long-acting injection for livestock and a preparation method thereof, belonging to the field of veterinary drugs. Each 100 ml of the injection comprises 10 to 20 g of enrofloxacin, 3 to 5 ml of a cosolvent, 0.01 to 0.5 g of a complexing agent, 3 to 10 g of a high-molecular slow release material, 0.05 to 1 ml of an antiseptic, 10 to 30 ml of an organic solvent and 0.1 to 0.2 g of an anti-oxidant, with the balance being injection water, and the pH value of the injection is 4 to 7. The invention provides veterinary clinical practice with the high-content weak acidic enrofloxacin long-acting injection and the preparation method thereof; the injection has the advantages of high content of enrofloxacin, small irritation, good stability and a long-acting effect; the preparation method is simple and is convenient for large scale production.

The invention discloses a process for producing masked Enrofloxacin comprises the following steps, (1) charging gelatin into water, (1) thermally insulating 0.5-1 hour at 30-50 deg C., charging glycerin, thermally insulating at 30-50 deg C, stirring to obtain gelatine size, (2) charging 6% of p-hydroxybenzoic acid ethyl ester ethanol solution, mixing to form gelatin-plasticizer composite solution, adjusting the pH to 6.8-7.2 with alkali, (3) charging water into autoclave, heating to 70-80 deg C, charging Enrofloxacin, stirring and charging silicon dioxide, stirring to form turbid liquor, cooling down to 45-55 deg C, adjusting pH to 6.8-7.2 with alkali, (4) charging gelatin-plasticizer composite solution into the turbid liquor, hardening 25-35 minutes with 5.37% of formaldehyde solution.

The present invention relates to an enrofloxacin gelatin microsphere and its preparation method. It is characterized by that it uses type A gelatin as carrier, uses enrofloxacin as medicine to be carried and adopts an emulsification-condensation process to prepare the invented gelatin microsphere which can take the lung as target organ after intravenous injection. The average grain size of said microspheres is 12 micrometers.

The invention relates to compound enrofloxacin pellets and a preparation method of the compound enrofloxacin pellets, wherein the enrofloxacin pellets comprise coatings and medicine-carrying pill cores; the coatings wrap the medicine-carrying pill cores; the medicine-carrying pill cores are prepared by the following steps that: amoxicillin and enrofloxacin are mixed with starch, microcrystalline cellulose, carboxymethyl starch and the like uniformly; an appropriate amount of pelleting adhesive is added in the mixture to prepare the soft material, the soft material is added into an extrusion pelleter to be extruded out, the extruded material is placed in a shot blasting machine to prepare the medicine-carrying pill cores. Then, the pill cores are placed into a fluidized bed to be dried andthen sieved; finally the sieved pill cores are placed into the fluidized bed, coating liquid is injected for coating, fluidized drying is then carried out after the coating, and then the finished product is obtained. According to the invention, both the amoxicillin and enrofloxacin are used, so that double effects are achieved, the medicine administration frequency and dose are reduced, and the pellets are easy to take only by stirring, and feed intake of animals is not influenced. In addition, the preparation method of the compound enrofloxacin pellets has simple process steps and low energyconsumption, is safe to produce and easy to implement, and the prepared pellets have uniform granularity.

The invention discloses a rabbit monoclonal antibody based ciprofloxacin residue analysis enzyme-linked immune adsorption kit. In the ciprofloxacin detection, the IC50 value of the kit is 1.41ng/mL, the linear fitting equation of an inhibition ratio curve is y = 0.0239x - 1.3159, the detection range is between 0.19 and 10ng/mL, and the lowest detection limit is 0.095ng/mL. The ciprofloxacin antibody in the kit has higher specificity, and has lower crossing-over rate with other quinolone micromolecules; and the cross reaction rates between the ciprofloxacin antibody and enrofloxacin, ofloxacin, norfloxacin, fleroxacin or pefloxacin are 28.8 percent, 13.1 percent, 11 percent, 22.6 percent and 20.4 percent respectively. The ciprofloxacin antibody has no cross reaction with other antibiotics and sulfanilamide medicaments. The kit is suitable for detecting ciprofloxacin residue in samples of milk, urine and the like. The kit can detect samples on a large scale at the same time, is convenient and quick, has quite important realistic significance for on-site supervision and trace analysis, meanwhile greatly reduces the detection cost, and has potential economic value.

The invention belongs to the environmental material preparation technical field, and relates to a preparation method of a photodegraded enrofloxacin hydrochloride floating type magnetic conductive surface molecular imprinting composite photocatalyst and an application. According to the invention, fly ash is modified, the floating type carboxyl modified fly ash hollow microspheres can be prepared, chitosan is used to perform a crosslinking preparation on magnetic Fe3O4 nano-particles to prepare the magnetic fly ash hollow microspheres, a sol-gel method is used to prepare the TiO2@magnetic fly ash hollow microspheres photocatalyst through photo-initiation polymerization, and the fly ash hollow microspheres photocatalyst is performed with ultrasonic modification, the template molecule enrofloxacin hydrochloride is added for photo polymerization, and is eluted, and leached by absolute ethyl alcohol and then dried. The prepared floating type magnetic conductive surface molecular imprinting composite photocatalyst can be used for degrading the enrofloxacin hydrochloride; the surface molecular imprinting composite photocatalyst has high selectivity on enrofloxacin hydrochloride after photocatalytic degradation no matter in a single-phase antibiotic solution or in a binary mixed phase antibiotic solution; and the magnetic separation characteristic enables convenience and high efficiency for separating and recovering.

The invention discloses a preparation method of a ciprofloxacin and enrofloxacin residue freeze-dried powder standard sample in carp muscle, which takes the carp as a research object to research a carp muscle material object standard sample containing ciprofloxacin and enrofloxacin of certain concentration in a breeding mode of adding target medicine. The key production control technology of the material object standard sample disclosed by the invention is mastered and comprises the control of content level, evenness, stability, constant value and the like, and the standard sample is produced and prepared in a standardization and scale mode. The standard sample is used for detection quality control and detection capability evaluation for residue of veterinary drug in animal-derived food and has an important significance on food safety detection.

The invention relates to a berberine hydrochloride compound preparation made from the following components in terms of weight percentage: 0.5-1% of berberine hydrochloride, 2-5% of antibacterial medicament, 0-0.1% of antiviral medicament, 1-1.5% of antibacterial synergist, 0-15% of stabilizer, 0-30% of cosolvent, 0-5% of local anodyne, 0-2% of antioxidant and balance of disperse medium. The inventive compound preparation has the following advantages: (1) the compound preparation solves the instability problem of berberine injection by making the berberine into orally taken liquid; (2) the berberine hydrochloride when used with enrofloxacin and TMP together can be used as the preferable medicament for intestinal infections, is more advantageous in treating intestinal infections caused by escherichia coli and salmonella than other single antibiotics sold in the market, especially good for drug resistant strains, is applied two times a day for three constant days, and prevention and treatment rate is up to 100%; (3) the berberine hydrochloride when used with enrofloxacin, gentamicin sulphate and TMP together is used to treat septicaemia, respiratory tract infections and intestinal infections caused by sensitive bacteria.

The invention discloses a method for preparing solid enrofloxacin nano particles, which is characterized by comprising the following steps: firstly, preparing 1 percent aqueous solution of glacial acetic acid with distilled water, and adding chitosan into the aqueous solution under a condition of magnetic stirring to dissolve the chitosan and obtain 1.5 to 2.5mg/mL solution of chitosan; secondly, adding enrofloxacin raw material medicament into the solution of chitosan, continuing performing magnetic stirring for 10min to uniformly disperse the enrofloxacin raw material medicament, adjusting the pH value to 5.0 to obtain solution of enrofloxacin chitosan; thirdly, dissolving sodium tripolyphosphate in the distilled water to prepare 1.2 to 1.8mg/mL solution of sodium tripolyphosphate, slowly dripping the solution of sodium tripolyphosphate into the solution of enrofloxacin chitosan under the condition of magnetic stirring, after dripping is finished, continuing performing magnetic stirring for 1h, immobilizing to obtain enrofloxacin chitosan nano particle suspension; and finally, filling the immobilized enrofloxacin chitosan nano particle suspension into frozen bottles, pre-freezing the bottles for 2h at the temperature of 70 DEG C below zero, vacuumizing and freeze-drying the bottles at the temperature of 50 DEG C below zero to obtain the solid enrofloxacin nano particles.

The invention relates to a production method for tasteless enteric enrofloxacin microcapsules, which comprises the following technical steps: (1) dissolving enrofloxacin to alkali liquor and adding stearic acid in a molten state for a first envelop; (2) mixing with auxiliary materials for pelletizing and rounding, and adding a coating agent for a second layer of envelop in the course of rounding; and (3) baking semi-finished products to obtain finished products. The production method of the invention has the advantages that macromolecular substances are utilized to envelop the enrofloxacin to isolate the enrofloxacin from the external environment, thus not only covering the strong bitterness of the enrofloxacin, but also having no influence on feed palatability; and the enveloped enrofloxacin microcapsules are not dissolved in the acid environment and gradually dissolved in the alkaline environment of the intestinal tract so as to reach the purpose of slow release and prolong the action time of the enrofloxacin.

The invention relates to a fishery Chinese and western compound medicine composition as well as a premix compound, batch mixture and application thereof and discloses a fishery Chinese and western compound medicine composition. The fishery Chinese and western compound medicine composition comprises the following components, namely, rheum officinale, golden cypress, scutellaria baicalensis, coptis chinensis, radix bupleuri, liquorice, gypsum and enrofloxacin. The fishery Chinese and western compound medicine composition has remarkable curative effects and takes quick effects on common bacterial diseases, such as bacterial septicemia, gill rot and red skin disease of aquatic livestock. The fishery Chinese and western compound medicine composition is stable in quality, convenient to use and low in cost, has the advantages of small toxic and side effects, efficient broad-spectrum, safety, environmental friendliness and the like and can be used for effectively ensuring the health and safety of the aquaculture animals.

The invention relates to a veterinary compound sulfadiazine sodium injection and a preparation method thereof. The veterinary compound sulfadiazine sodium injection comprises sulfadiazine sodium, enrofloxacin, flunixin meglumine, trimethoprim, dexamethasone sodium phosphate, an organic solvent and water for injection. The injection serving as a veterinary special compound preparation has a special treating effect on various infectious diseases, mixed infections and systemic infections of the digestive system, the respiratory system, the urinary system and the skin soft tissue caused by sensitive bacteria and mycoplasmas of oxen, swines, poultries, canines, cats and aquatic animals The injection has the advantages of convenient use, short period of treatment, low medicament resistance and the like.

The invention relates to a method for preparing a molecularly imprinted polymer enriched with trace enrofloxacin, which comprises the main technical key point that enrofloxacin is used as a template molecule, 3-aminopropyl triethoxysilane is used as a functional monomer, tetraethoxy silane is used as a crosslinking agent, an activated silicon ball is used as a carrier, N,N-dimethylformamide is used as a pore-foaming agent, and a surface molecular imprinting technique and a sol-gel technique are utilized to synthesize an adsorptive functional material having high selectivity to the enrofloxacin. The method has a low cost and a simple synthetic process, the reaction condition is easy to control, and the prepared enrofloxacin molecularly imprinted polymer can be used for the combination of solid phase extraction enrichment and high efficiency liquid chromatography, and is suitable for performing online detection on the trance enrofloxacin in animal-based foods and performing purification treatment on various samples.