60 results about "Enrofloxacin" patented technology

This invention provides a composite aerogel based on Eucommia ulmoides gum and MXene nanosheets, its preparation method, and its application. The aerogel is prepared by reacting a toluene solution of Eucommia ulmoides gum with Ti3C2T... x MXene aqueous solution was mixed and a water-in-oil microemulsion was formed using a combination of ultrasound-assisted shaking. This allowed MXene nanosheets to self-assemble at the microemulsion droplet interface. Following vertical freezing and freeze-drying processes, a three-dimensional network structure of Eucommia ulmoides gum-Ti3C2T was successfully constructed. x MXene composite aerogel. The preparation method of this invention is mild and requires no chemical cross-linking agents. The resulting composite aerogel exhibits excellent adsorption performance for enrofloxacin, malachite green, Congo red, rhodamine, and kanamycin in water, with adsorption equilibrium times all within 10 minutes, enabling rapid removal of organic pollutants from water. This invention provides a new approach for the high-value utilization of Eucommia ulmoides gum and has promising application prospects in the field of water environment remediation.

A fermented traditional Chinese medicine feed additive for catfish, its preparation method, and its application are disclosed, relating to the field of aquatic feed additives. The preparation method involves activating and expanding the culture of *Saccharomyces cerevisiae*, *Bacillus subtilis*, and *Lactobacillus plantarum*; pulverizing *Paeonia suffruticosa* leaves, *Smilax china*, *Scutellaria baicalensis*, *Rhus chinensis*, hawthorn, *Polygonum cuspidatum*, ginkgo, and forsythia suspensa* to obtain traditional Chinese medicine powders; preparing a solid fermentation medium using the traditional Chinese medicine powders, soybean meal powder, corn cob granules, and wheat bran; inoculating *Saccharomyces cerevisiae* seed culture and *Bacillus subtilis* seed culture for aerobic fermentation; and inoculating *Lactobacillus plantarum* seed culture for anaerobic fermentation to obtain the fermented traditional Chinese medicine feed additive. The resulting fermented traditional Chinese medicine feed additive promotes the absorption and metabolic elimination of enrofloxacin in catfish, shortens the withdrawal period, has preventive and adjunctive therapeutic effects on bacterial diseases in catfish, promotes growth, reduces feed conversion ratio, and improves survival rate.

The invention discloses a method for ratio-dependent fluorescence detection of enrofloxacin based on a new composite nano carbon dot-gold cluster material and application, and belongs to the technical field of advanced non-ferrous metal nano new materials and new material detection. The new composite nano carbon dot-gold cluster material AuNCs-CDs can be prepared by mixing gold nano cluster seeds and ascorbic acid according to a certain proportion and carrying out microwave heating; under the same excitation wavelength, the AuNCs coated CDs have two emission peaks of 440 nm and 640 nm. The method comprises the following steps: adding enrofloxacin with different concentrations into an AuNCs (at) CDs solution, and measuring the fluorescence intensity ratio I440 / I640 of the AuNCs (at) CDs; and drawing a standard curve according to the relationship between the concentration of the enrofloxacin solution and the fluorescence intensity ratio of the AuNCs coated CDs solution, so as to detect the concentration of the enrofloxacin. In addition, the method can be applied to experimental recovery rate detection of enrofloxacin in an actual sample. The method disclosed by the invention has high sensitivity and selectivity, is simple and rapid, does not need any pretreatment, and shows an extremely wide application prospect in many fields such as biomedical detection, environmental monitoring, food safety analysis and the like.

The present application belongs to the technical field of wastewater treatment, and particularly relates to a seawater breeding wastewater antibiotic removal method based on oyster shell composite filler. Fine gravel, oyster shell and functional mineral are compounded in a fixed volume ratio of 2:1:1, and are laid in a structure with a limited particle size range and homogeneity without stratification. A salinity-directed salt-tolerant domestication process and a tidal intermittent operation mode of 48h hydraulic retention + 24h emptying and reoxygenation are matched, and salt-tolerant functional bacterial flora of Proteobacteria, Paracoccus and Shigella are enriched. The present application can adapt to a wide range of salinity conditions of 10‰-30‰, relieve high salt inhibition and ion competition, simultaneously and efficiently remove three typical antibiotics of conventional pollutants, sulfamethoxazole, trimethoprim and enrofloxacin, and reduce resistance genes. The sulfamethoxazole removal rate can reach 93.94% under the condition of 30‰ high salinity, the system runs stably and has strong adaptability, and has certain engineering application value and large-scale popularization potential in the field of seawater breeding wastewater treatment.

The application discloses a small-molecule multi-connection detection kit based on a biochip, a preparation method and a detection method thereof. The kit comprises nano-gold particle labeled antibodies, a multi-connection detection chip plate and a detection buffer. The nano-gold particle labeled antibodies comprise nano-gold particle labeled florfenicol antibodies and enrofloxacin antibodies. The multi-connection detection chip plate is coated with florfenicol antigens and enrofloxacin antigens in detection holes. The detection buffer is composed of 10-40 mM phosphate buffer and 0.05-0.5 wt% surfactant S17. The application can simultaneously detect florfenicol and enrofloxacin small molecules by using one detection buffer, thereby saving time and labor.

The application discloses a kind of enrofloxacin rapid detection methods based on fluorescent covalent organic framework magnetic beads, belong to food safety detection field.The method uses the fluorescent covalent organic framework magnetic bead of coupling enrofloxacin monoclonal antibody, realizes detection in combination with immunochromatography test strip.Fluorescent covalent organic framework magnetic bead realizes target enrichment separation with inner core, shell fluorescent covalent organic framework material high-efficiency coupling antibody and exports fluorescence signal;Test strip is equipped with detection line (coated enrofloxacin artificial antigen) and quality control line (coated secondary antibody).By comparing the fluorescence intensity of detection line and quality control line, trace enrofloxacin in complex matrix can be qualitatively and quantitatively analyzed.The method is simple, rapid and sensitive, without large instrument, can be detected on site.

The invention discloses a light-emitting probe based on a europium-based metal-organic framework and a preparation method and application thereof, and belongs to the technical field of fluorescent sensing materials and food safety detection.The light-emitting probe is a europium-based metal-organic framework material, the chemical formula of the light-emitting probe is Eu (phen) (5-hipa) NO3 <->, phen is 1, 10-phenanthroline ligand, 5-hipa is 5-hydroxyisophthalic acid ligand, and the molecular weight of the Eu (phen) (5-hipa) NO3 <-> is larger than that of Eu (phen) (5-hipa) NO3 <->. The light-emitting probe takes a binuclear Eu cluster as a metal center. According to the luminous probe based on the europium-based metal-organic framework, the synthesized europium-based metal-organic framework material can be used as a bifunctional fluorescent probe, and specific recognition and quantitative detection of carbaryl and enrofloxacin are realized in an aqueous solution; the method has the remarkable effects of high sensitivity, strong anti-interference capability, recyclability and simplicity, convenience and rapidness in operation.

The invention discloses construction of a self-energized sensor based on HOF-on-MOF and application research of enrofloxacin in mandarin fish, and belongs to the field of self-energized sensing, a photocathode material and a photoanode material required by the self-energized sensor are prepared by applying a hydrothermal synthesis method, the self-energized sensor is constructed by utilizing the photocathode material, and the self-energized sensor is used for detecting enrofloxacin in mandarin fish. An aptamer (Apt) is successfully combined to a photocathode, the self-powered photoelectrochemical aptamer sensor is constructed, the photocurrent value of the self-powered sensor is linearly related to the logarithm of the concentration of enrofloxacin (ENR), the photocurrent value after the ENR is added is substituted into a linear regression equation of a standard curve drawn by the photocurrent and the logarithm of the concentration, and the self-powered photoelectrochemical aptamer sensor is constructed. Compared with an existing ENR detection method, the method has the advantages that the detection range is wider (1.0 * 10 <-11 >-1.0 * 10 <-6 > mol / L) and the detection limit is lower (3.3 * 10 <-12 > mol / L), and trace detection of the ENR can be realized.

The application discloses a compound enrofloxacin injection, which contains 10-25g of enrofloxacin, 2-10g of sulfadimoxine sodium, 5-12g of florfenicol, 0.5-2.5g of bromhexine hydrochloride, 1-20g of glacial acetic acid, 2-5g of Tween 80, 5-15g of alpha-pyrrolidone and 5-20g of N, N-dimethylformamide per 100ml of the injection, and the rest is water for injection. The compound enrofloxacin injection has advanced and reasonable prescription design, stable and reliable preparation process, good preparation stability and small irritation, and can achieve the effect of treating all samples for respiratory system diseases of livestock and poultry.

The application belongs to the field of electrochemical detection, and particularly relates to a novel water-vapor coupling photoelectric self-powered electrochemical sensor for detecting enrofloxacin and a preparation method thereof. A glass substrate is used as a substrate, a copper foil tape is used as a positive electrode and a negative electrode, CdS (cadmium sulfide) and a cobalt-based metal organic framework (ZIF-67) are used as water-vapor / photovoltaic materials to construct a heterojunction for preparing a water-vapor generator to realize sustainable power generation. The CdS / ZIF-67 heterojunction is beneficial to the separation of photo-generated carriers under illumination and synergistically enhances the water evaporation effect. An enrofloxacin (ENR) aptamer is assembled to construct a water-vapor / photovoltaic self-powered electrochemical sensor. With the help of split aptamers, the ENR detection exhibits high sensitivity and high specificity, and provides a new strategy for self-powered electrochemical detection. The water-vapor / photovoltaic self-powered electrochemical sensor has a wide detection range, high sensitivity and low detection limit.

The application provides enrofloxacin suspension and a preparation method and application thereof, and is characterized in that, according to 100% by weight, the enrofloxacin suspension comprises the following components: enrofloxacin 4%-6%; sodium carboxymethyl cellulose 0.4%-0.6%; hydroxyphenyl ethyl ester 0.04%-0.06%; and purified water is supplemented to 100%. The drug particle size D90 in the enrofloxacin suspension is 2-10 microns. By limiting the weight ratio of each component and the synergistic effect between the formula, and by matching the drug particle size range of the suspension, the following comprehensive technical effects are achieved: long-acting slow release, low irritation, applicability to pregnant animals, multi-pathway drug delivery, low sedimentation rate, and almost no stratification.

The application discloses an anti-ENR nanobody, a coding gene, a cELISA detection method and purposes, and belongs to the technical field of biology. The amino acid sequence of the anti-ENR nanobody is shown as SEQ ID NO. 1, and the nucleic acid sequence for coding the anti-ENR nanobody is shown as SEQ ID NO. 2. A new type of competitive ELISA (cELISA) is established by screening the specific nanobody against ENR from an immunized bactrian camel library. When detecting the enrofloxacin residue in animal-derived food, the method has high sensitivity and specificity, can detect the ENR residue as low as 6.49 ng / mL in the sample, and has no cross reaction with other antibiotics. It is shown that the cELISA established in the application is a simple, rapid, sensitive and low-cost immunodetection method, and has great application prospect in the detection of ENR residue in animal-derived food.

The spray drying device comprises a bottom plate, the middle side of the upper end of the bottom plate is connected with a machine body, the lower side of the interior of the machine body is connected with a hot air pipe, and the lower side of the machine body is connected with a discharging pipe; an atomizer is arranged on the middle side of the upper end of the machine body, a stirring structure is connected to the middle side in the machine body, and the stirring structure comprises a connecting plate, a motor and a connecting wheel; and the connecting pipe is mounted on the outer side of the hot air pipe, the upper side of the connecting pipe is connected with a mounting plate, the outer side of the upper end of the mounting plate is connected with a flow guide plate, a scraping structure is mounted on the outer side of the mounting plate, and the scraping structure comprises a scraping plate and a fixing plate. According to the spray drying device for preparing the enrofloxacin water emulsion particles, the stirring structure is arranged, mass transfer can be promoted, the material uniformity can be guaranteed, and therefore the drying effect of enrofloxacin water emulsion is improved, and the scraping structure is arranged, so that the inner wall of the drying device can be cleaned.

The application belongs to the technical field of veterinary drug excipients, and particularly relates to a water-soluble veterinary drug excipient and a preparation method and application thereof. The water-soluble veterinary drug excipient comprises the following components in parts by mass: 70-80 parts of magnesium sulfate, 30-40 parts of fermented cassava starch, 0.1-1 part of polyvinylpyrrolidone, 0.5-3 parts of a surfactant, 1-2 parts of a pH regulator, and 0.1-1 part of a sweetening agent. The fermented cassava starch is prepared by fermenting cassava raw starch prepared from fresh cassava by using composite bacteria, and the composite bacteria comprise Bacillus velezensis, Bacillus subtilis and Bacillus amyloliquefaciens. The water-soluble veterinary drug excipient prepared by the application can improve the dissolution rate and storage stability of enrofloxacin.

The invention provides a method for evaluating an activated sludge system response mechanism under antibiotic impact and application thereof, and belongs to the technical field of water environment risk evaluation and management. The method mainly relates to detection of water quality indexes (ammonia nitrogen, total nitrogen, total phosphorus and chemical oxygen demand) in the activated sludge and physicochemical characteristics (extracellular polymeric substance EPS, sludge settling performance and Zeta potential) of the activated sludge, and a response mechanism of an activated sludge system under antibiotic impact is analyzed by taking ciprofloxacin (CIP), ofloxacin (OFL) and enrofloxacin (ENR) as indication pollutants. Effective reference is provided for system regulation and control when a sewage plant faces antibiotic impact, and a new insight is provided for removing antibiotics and drug-resistant genes in wastewater by explaining the relation among functional genes, drug-resistant genes and microbial communities in an activated sludge system under antibiotic exposure.

The application provides a composite material with adsorption-photocatalysis synergistic effect and a preparation method and application thereof, relates to the technical field of materials, and comprises the following steps: mixing ethylene glycol and bismuth nitrate to form a solution, slowly adding a sodium molybdate and sodium tungstate aqueous solution, then adding titanium dioxide, mixing, and then carrying out constant-temperature reaction in a high-pressure reaction kettle; and then sequentially carrying out suction filtration, washing, and vacuum drying to obtain Bi2W x Mo 1‑x O6 / TiO2. x Mo 1‑x O6 / TiO2 is added into a dispersion solution of graphene oxide, ultrasonic reaction is carried out, urea is added, and continuous ultrasonic reaction is carried out; the obtained mixed solution is moved into a high-pressure reaction kettle for constant-temperature reaction; after the reaction is completed, sequential suction filtration, washing, and vacuum drying are carried out to obtain Bi2W x Mo 1‑x O6 / TiO2 / rGO material. The application has the advantages of convenient preparation, low energy consumption, high yield, cleanness, environmental protection, high adsorption-photocatalysis synergistic removal activity of the obtained product to organic matters such as enrofloxacin and rhodamine B, excellent repeated use performance, and good application prospect in the field of organic wastewater purification treatment.

The invention discloses a porous carbon electrode based on gold nanoparticles / poly (3, 4)-ethylenedioxythiophene, a preparation method of the porous carbon electrode and application of the porous carbon electrode in antibiotic detection, and belongs to the technical field of food. The preparation method based on the gold nanoparticle / poly (3, 4)-ethylenedioxythiophene / porous carbon electrode comprises the following steps: (1) engraving the porous carbon electrode by using a laser engraving machine; (2) putting the carbon electrode into a (3, 4)-ethylenedioxythiophene solution, and polymerizing (3, 4)-ethylenedioxythiophene on the porous carbon electrode by utilizing a potentiostatic method; (3) placing the electrode prepared in the step (2) in a chloroauric acid solution, and depositing gold nanoparticles on the electrode through cyclic voltammetry; the gold nanoparticle / poly (3, 4)-ethylenedioxythiophene / porous carbon electrode provided by the invention can sensitively detect chloramphenicol and enrofloxacin at the same time, and can be used for detecting chloramphenicol and enrofloxacin in tap water.

The application discloses a method for rapidly removing enrofloxacin in a breeding tail water treatment technology "three pools and two dams" by micro-nano aeration in an aeration tank in cooperation with calcium peroxide, and specifically comprises the following steps: installing a micro-nano bubble generating device, placing the water inlet end of a water inlet pipe and the water outlet end of a water outlet pipe of the micro-nano bubble generating device below the liquid level in the aeration tank, and starting the micro-nano bubble generating device; spraying calcium peroxide; after the aeration tank stably generates micro-nano bubbles, spraying calcium peroxide in the aeration tank to degrade enrofloxacin in the breeding tail water in the aeration tank at a concentration of μg / L level. The breeding tail water is circulated through the micro-nano bubble generating device, a large number of micro-nano bubbles are generated, and the oxygen-producing agent calcium peroxide which is frequently used in breeding management activities is added. The calcium peroxide is non-toxic, can release oxygen when meeting water, can rapidly generate high-activity oxygen in the water body, can increase the dissolved oxygen content of the water body, can generate a large number of free radicals, can improve the oxygen mass transfer efficiency, and can rapidly and effectively remove enrofloxacin in the breeding tail water.

The invention belongs to the technical field of biochar preparation, and discloses potassium carbonate modified enteromorpha biochar as well as a preparation method and application thereof. N and S element doped potassium carbonate modified enteromorpha biochar with high specific surface area is prepared through two-step heating, activation and pyrolysis, so that the biochar is promoted to form a rich pore structure, and the adsorption capacity is improved. The modified biochar prepared by the method is large in specific surface area and rich and uniform in pores, has the adsorption capacity remarkably superior to that of a conventional carbon material, can be applied to actual wastewater to remove antibiotic pollutants, can well adsorb and remove enrofloxacin, is beneficial to ecological environment protection, and has relatively large economic benefits, social benefits and environmental benefits.

The application discloses a phosphorus-sulfur synergistic antibiotic bacterial residue biochar, a preparation method and application thereof, and belongs to the technical field of biochar. The application takes penicillin bacterial residue and high-sulfur wet-process phosphoric acid as raw materials, and prepares biochar through phosphorus-sulfur synergistic modification and applies the biochar to adsorption of antibiotic wastewater, so that efficient purification of the antibiotic wastewater and full-resource utilization of the bacterial residue are realized. The method innovatively uses cheap high-sulfur wet-process phosphoric acid to replace traditional strong acid (hydrochloric acid, sulfuric acid, etc.) for pretreatment, and the obtained acid hydrolysis solution can be directly used for preparing fertilizer, thereby simultaneously reducing pyrolysis cost and improving nitrogen utilization rate. The phosphorus-sulfur in-situ modification process makes the carbon yield of the biochar increase by 10%, the specific surface area increase by 45%, and the resistant genes in the bacterial residue are completely eliminated. The prepared biochar has an antibiotic removal rate of more than 99% for antibiotic wastewater such as enrofloxacin and tylosin, and the wastewater COD and ammonia nitrogen removal rates reach 96% and 71% respectively, so that the high-value utilization of antibiotic bacterial residue is realized while the environmental safety is ensured.

The application discloses a preparation method of a drug-resistant preventing enrofloxacin hydrochloride compound powder, and belongs to the technical field of medicine preparation. The drug-resistant preventing enrofloxacin hydrochloride compound powder comprises the following raw materials in mass fractions: enrofloxacin hydrochloride 1-30 parts, gallic acid 2-46 parts, a solubilizer 0.5-9 parts, an absorption promoter 0.5-9 parts and starch 0-92 parts. The drug-resistant preventing enrofloxacin hydrochloride compound powder can treat various disease infections of aquatic animals, and the gallic acid in the powder can greatly reduce the drug resistance of pathogenic bacteria to enrofloxacin hydrochloride and enhance the antibacterial activity of enrofloxacin hydrochloride.

The application relates to the chemical technology field, in particular to a compound of a ciprofloxacin intermediate, a preparation method and application thereof. The method comprises the following steps: carrying out N-cyclopropylization reaction on 3-chloro-4-fluorobromobenzene and cyclopropylamine in the presence of a composite catalyst to obtain N-cyclopropyl-3-chloro-4-fluoroaniline; and carrying out cyclization and hydrolysis on the N-cyclopropyl-3-chloro-4-fluoroaniline and EMME to obtain cyclopropyl carboxylic acid. The method has the advantages of easy raw material, simple process steps, avoidance of use of dangerous or expensive reagents, mild reaction conditions, suitability for industrial production, high purity of the intermediate compound prepared through the method, direct use of the intermediate compound for efficient synthesis of important quinolone antibacterial drugs such as ciprofloxacin and enrofloxacin, and important application value.

The application discloses application of a filamentous microalgae in removal of enrofloxacin in water bodies. The application is obtained by the inventors of the application who find that Nostoc sp.SCAU-13 has the effect of removing enrofloxacin in water bodies. The algae strain can grow in water bodies containing enrofloxacin and can efficiently remove enrofloxacin, which indicates that the algae has the application potential in removing antibiotics in sewage. The application not only provides a biological treatment method of enrofloxacin, but also is easy to collect and separate from water bodies, so that subsequent treatment of the water bodies is simple.

This application provides a *Staphylococcus pseudoci* AT8 strain for degrading antibiotics and its application. The *Staphylococcus pseudoci* is a species of Staphylococcus (…). Staphylococcus The *Staphylococcus pseudocarpa* species, having a 16S rDNA sequence as shown in SEQ ID NO:1, is named... Staphylococcus pseudoxylosus AT8, deposited on November 20, 2025 at the Guangdong Provincial Center for Microbial Culture Collection, accession number: GDMCC No: 67323. This application pertains to *Staphylococcus pseudocarpa*. Staphylococcus pseudoxylosus AT8 can achieve a degradation rate of over 90% for trimethoprim and tilmicosin, and over 78% for enrofloxacin. Furthermore, this application enriches the antibiotic-degrading bacterial strain library, promotes the control of new antibiotic pollutants in livestock and poultry manure and farmland soil, and facilitates the resource-based treatment and safe utilization of solid waste, demonstrating broad application prospects.

This invention discloses an electrochemical biosensor and electrochemical detection method for enrofloxacin. The electrochemical biosensor includes an electrode and G-quadruplex ribozyme nanowires coated on the electrode. The G-quadruplex ribozyme nanowires are obtained by amplification and extension after complementary hybridization of a single-stranded R-DNA probe and an S-DNA probe pre-modified on the electrode surface. The single-stranded R-DNA probe is obtained by releasing a DNA double-stranded probe, which is obtained by partial complementary hybridization of an R-DNA probe with a free 3'-OH end and an enrofloxacin aptamer sequence. This invention establishes an electrochemical detection method for enrofloxacin based on a signal amplification strategy designed by terminal deoxynucleotidyl transferase (TdT) in conjunction with G-quadruplex ribozyme. The linear detection range of this method for enrofloxacin is 0.5–50 ng / mL, with a detection limit as low as 0.043 ng / mL. Furthermore, this label-free electrochemical biosensor is simple, rapid, and low-cost, and has been successfully applied to the analysis and detection of actual food samples, showing good application potential.

The invention provides a preparation method of a quinolone product for inhibiting generation of nitrosamine impurities, and belongs to the technical field of quinolone medicine preparation. The quinolone product is ciprofloxacin, ciprofloxacin lactate or enrofloxacin, and a nitrosamine inhibitor is added in the preparation process of ciprofloxacin, ciprofloxacin lactate or enrofloxacin to obtain the corresponding quinolone product. The nitrosamine inhibitor is any one of ascorbic acid, alpha-tocopherol, maltol, resveratrol, propyl gallate, butylated hydroxyanisole, butylated hydroxytoluene, caffeic acid, ferulic acid, alpha-amino acid, L-cysteine, para aminobenzoic acid, urea and ammonium chloride. According to the method disclosed by the invention, the content of nitrosamine impurities can be reduced to be within 0.3 ppm.

The application discloses a compound enrofloxacin-nano silver emulsion and a preparation method and application thereof, and the preparation method comprises the following steps: uniformly dispersing ganoderma extract and silver nitrate in deionized water, and reacting at constant temperature of 60-90 DEG C in dark to obtain a nano silver solution; taking enrofloxacin, lauric acid monoglyceride and oleic acid, heating and melting, and uniformly stirring to obtain an oil phase; dispersing polyglycerol-10 stearate in a polyvinyl alcohol solution, and heating and stirring to obtain an aqueous phase; under the condition of constant temperature stirring, the oil phase is transferred into the aqueous phase, and a high shear instrument is used for shearing to obtain a coarse dispersion; the coarse dispersion is treated by using a high-pressure homogenizer to obtain an enrofloxacin emulsion; the enrofloxacin emulsion is uniformly mixed with the nano silver solution, and is cooled to obtain the compound enrofloxacin-nano silver emulsion. The application can improve the solubility of enrofloxacin, has good characterization performance, the preparation cost is controllable, macro-preparation can be realized, and the compound emulsion can effectively treat animal skin infection and promote wound healing.

The invention belongs to the technical field of veterinary drugs, and discloses a preparation method and application of pH response type enrofloxacin granules for treating porcine respiratory bacterial mixed infection. The invention provides the enrofloxacin coated particle, the enrofloxacin coated particle has pH response capability, is not released in a stomach pH environment and can be quickly released in a near small intestine pH environment, so that the stomach irritation of enrofloxacin is reduced, and the enrofloxacin is ensured to be absorbed at an optimal part. According to the enrofloxacin coated granules, the bitter taste of the medicine is effectively covered, the administration frequency is reduced, and the effective plasma concentration maintaining time is prolonged. Meanwhile, the enrofloxacin coated granules have a good treatment effect on porcine respiratory diseases caused by mixed infection of various bacteria.

The invention discloses an intelligent dynamics modeling method and system for enrofloxacin solution stability prediction, and relates to the technical field of intelligent models. Comprising the following steps: associating influence data in an enrofloxacin solution transportation process according to a time sequence; a delay response factor is introduced, the dynamic relation between the influence data change and the solubility of the enrofloxacin solution is adjusted, the solubility of the enrofloxacin solution is reflected by an Arrhenius reaction kinetic model, and the Arrhenius reaction kinetic model is associated with the delay response factor to generate a time delay kinetic model. According to the method, on the basis of the Arrhenius reaction kinetic model in the prior art, the delay response factor is added, and the time delay kinetic model is constructed, so that the defect of the Arrhenius reaction kinetic model in the aspect of processing the nonlinear lag relationship is overcome; the stability of the solubility of the enrofloxacin solution can be predicted in the transportation and storage process of the enrofloxacin solution.

The invention discloses an anti-enrofloxacin nano antibody and application thereof, and belongs to the technical field of nano antibodies. The invention aims to provide a nano antibody which is small in molecular mass, high in stability, strong in penetrating power, low in production cost and capable of being specifically combined with enrofloxacin. The invention provides a nano-antibody for detecting enrofloxacin. The amino acid sequence of a CDR1 of the nano-antibody is shown as SEQ ID NO.6, the amino acid sequence of a CDR2 of the nano-antibody is shown as SEQ ID NO.7, and the amino acid sequence of a CDR3 of the nano-antibody is shown as SEQ ID NO.8. The invention further provides a preparation method of the nano-antibody for detecting enrofloxacin. The indirect competitive ELISA method established by the invention has relatively high sensitivity, precision, specificity and correctness, provides a new technical support for detection of enrofloxacin residues in animal-derived food, is simple, rapid, sensitive and low in cost, and can effectively detect the enrofloxacin residues in the animal-derived food.