202 results about "Malachite" patented technology

Compounds and procedures are provided for use in the preparation of reagents that can be used in immunoassays for Malachite Green, Leucomalachite Green and Carbinol. The compounds are Malachite Green Chromatic derivatives conjugated to several positions of the Malachite Green Chromatic structure, which in turn are conjugated to antigenic polypeptides for formation of antibodies for use in immunoassays. The antibodies produced, when employed in immunoassays with specified procedures, are found to be able to detect Malachite Green Chromatic as determined by positive results with fish tissue and water sample containing Malachite Green Chromatic that has been detected by HPLC or LC-MS.

The invention relates to a colorless green malachite green indirect compete ELISA test agent box of an aquatic product. The test plate of the agent box is a split 96 holes enzyme mark plate which coats colorless green malachite green and albumin coupling material; the anti-colorless green malachite green antibody is a monoclonal antibody which is prepared by the colorless green malachite green and the albumin coupling material immune mouse; the tested sample uses ethyl hexoate and cyclohexane to extract after uniform and adjusts PH value for detecting. The quoting standard of the sample detecting is that it is a regression curve which uses the logarithm value of the sample density as abscissa and uses the degradation rate as ordinate. We could read the density of the corresponding sample from the degradation rate of each sample. The sensibility of the test method is 0.0023ª–g / ml; the test range is 0.0016-1ª–g / ml.

The invention discloses an enzyme immunoassay of testing the bice green residues in animal derived food, which comprises an enzyme label plate covering bice green antigen, enzyme label bice green antibody working solution, bice green standard solution, substrate solution, substrate buffer solution, reaction termination solution, concentration washing liquid and sample dilute solution. The invention further discloses a method for using the immunoassay to test bice green residues, which comprises sample pretreatment, testing via the immunoassay, processing and analyzing result. The inventive immunoassay of bice green test uses direct competition enzyme-linked immunoassay adsorption analysis technique, with high sensitivity, high stability, simplified operation, reduced reaction time, reduced error caused by complex operation, reduced cost, wide application for testing samples and high practicality.

The invention provides a molecular imprinted polymer microsphere for fast detecting malachite green in aquatic products, which is prepared by mixing two functional monomers at a certain ratio, mixing a malachite green template with the monitor at a certain ratio, sequentially adding a crosslinking agent, an initiator, an emulsifying agent and a lipophilic solvent, and preparing the molecular imprinted polymer microsphere. The invention further provides a method for detecting the malachite green in a sample, which comprises the following steps of: identifying, adsorbing and concentrating a processed aquatic product, and determining the concentration of the malachite green by spectrophotometry. Additionally, the sample can be concentrated to lower the detection limit, so that the method is especially suitable for detection of low-content samples.

The invention discloses a method for treating malachite green dye waste water, which comprises the following steps of: preparing a bacterial cellulose membrane; modifying the bacterial cellulose membrane; immobilizing white rot fungi by using the modified bacterial cellulose membrane; adding the obtained immobilized white rot fungi modified bacterial cellulose membrane into 50 mL of the malachite green dye waste water; dissolving 20 to 40 mg of malachite green into 1 L of white rot fungi liquid restrictive culture medium; treating the waste water on a gas bath constant-temperature shaking table at a speed of 120 rpm for 5 days at 28 DEG C, and measuring the chroma of the waste water at intervals of 24 hours; 5 days later, discarding the treated waste water, and retaining the immobilized fungi; adding 50 mL of newly-prepared dye waste water with the same concentration, treating the waste water under the same condition, and measuring the chroma of the waste water at intervals of 24 hours; and taking 5 days as a cycle, and repeating the steps for 5 times, wherein the removal rate of the chroma of the waste water is always over 86 percent. In the method, the referred bacillus xylinus and the white rot fungi are purchased from the China General Microbiological Culture Collection Center.

The invention relates to a water body, a method for rapidly detecting the residue of malachite green and colorless malachite green in an aquatic product, belonging to the technical field of residue detection in a water body and an aquatic product. The method comprises the following steps: oscillating and mixing a sample to be detected and an extractant I uniformly; adding an extractant II, oscillating and mixing and then standing still; taking and adding an supernatant to an enrichment column; observing color change of a chromogenic reagent on the lowest layer; and determining that the sample contains the malachite green and colorless malachite green if a green circular strap is formed. The extractant I is a mixed solution of acetonitrile, dichloromethane, n-hexane and paratoluenesulfonic acid, the extractant II is a mixture of lead dioxide and acid alumina, and the sample to be detected is a water body sample to be detected or an aquatic product sample to be detected. The invention has the advantages of simple operation and fewer steps, does not need expensive instruments and equipment and can embody the convenience and the flexibility to on-site detection.

The invention discloses an enzyme linked immunosorbent assay method (ELISA) for detecting the total amount of diamond green and blank diamond green in measurement water sample and aquatic products, characterized in that: the modified compound of amido blank diamond green is synthesized and linked with the protein to produce immunogen and envelope antigen, and the polyclonal antibody of rabbit-anti colorless diamond green is obtained by immuning animal and the concentration range of ELISA standard curve is 0.1-100ng / mL, IC50 is 0.9-2.6ng / mL and the recovery of standard addition is 76.2-95%, the correlation coefficient of ELISA and HPLC is 0.975, n=7; because the cross-reaction rates of the prepared antibody and diamond green are respectively 95.25%, the ELISA can be used for measuring the total amount of the diamond green and blank diamond green without any oxidation step.

The invention discloses an ELISA kit for detecting recessive malachite green, comprising recessive malachite green polyclonal antibody, recessive malachite green hapten and complete antigen and enzyme-labelled antigen. The inventive ELISA kit is sensitive, fast and accurate, mainly used for screening of mass samples. Mainly reagent in the kit is provided in the form of work liquid, with the advantages of convenient usage, high specificity, high accuracy, high exactness and the like, for fast detecting recessive malachite green residual in the aquatic products.

The invention discloses a fast testing process of malachite green, which settles after mixed the measured sample and withdraw medicinal preparation, take the supernatant into the chromatography column, observes most lower level absorbent color change in the chromatography column, if forms the green clitellum, might judges that malachite green contains in the sample; the withdraw medicinal preparation is the mixture of acetonitrile and ethyl acetate solution and the acidic aluminum oxide, of which the volume ratio of the acetonitrile and ethyl acetate is 1: 15-30, the quality of the states measured sample and the mixture solution of acetonitrile and ethyl acetate volume ratio is 1:3; the quality ratio of the measured sample and the acidic aluminum oxide is 2:1. The invention used acetonitrile-ethyl acetate solution to withdraw the malachite green more completely and remarkably reduce the toxicity which is used in the reagent, also had stronger service ability to a fat product with higher content.

The invention provides an aptamer electrochemical sensor for detecting malachite green (MG). A preparation method for the electrochemical sensor comprises the steps of: the prepared graphene-chitosan compound and nanogold are modified on an electrode surface, an aptamer is connected on the nanogold through chemical action, a target object is bound onto the electrode surface through the specific binding action of the aptamer and the target and then connected with an antibody of the target object, thus constructing the biosensor with a sandwich structure. When the detected solution contains the target object, a certain amount of target objects and corresponding antibodies can be fixed on the electrode surface, so that a sensing structure is formed. Horseradish peroxidase (HRP) is modified on the antibodies, and the hydrogen peroxide can be catalyzed to be decomposed, so that the change of an electrochemical signal can be generated, and the detection on the MG can be realized through the change of the electrochemical signal. The prepared aptamer electrochemical sensor is strong in selectivity, high in sensitivity, simple and fast to operate, and suitable for detection on the MG of aquatic products.

The invention discloses an immunochromatographic test strip for detecting MG and a preparation process thereof. The immunochromatographic test strip for detecting MG provided in the invention comprises a sample pad containing MG antibodies marked by superparamagnetic composite particles, a cellulose nitrate membrane connected with one end of the sample pad and an absorbent connected with another end of the cellulose nitrate membrane; the cellulose nitrate membrane is coated by mutually separated detection lines and quality control lines, wherein, the detection lines contain MG antigens, and the quality control lines contain antiantibodies which can specifically bind to the MG antibodies. Experiments in the invention prove that the test strip provided in the invention has the advantages of high sensitivity, strong specificity, rapidness, simpleness and capability of realizing objective detection when is used for detecting MG.

The invention discloses an Elisa agent for detecting malachite green and a method thereof. The Elisa agent comprises a malachite green antigen or antibody coated Elisa plate, an enzyme label, a malachite green specific antibody, a malachite green standard solution, a substrate developing solution, a stop solution, a concentrated cleaning solution, enzyme label diluents and a concentrated combined solution. The Elisa agent is used for the quantitative detection of the content of the malachite green in fishes, shrimps and water samples and has the advantages of high specificity and sensitivity, easy sample pretreatment, short detection time, large sample detection amount, and the like.

The invention discloses a method for treating malachite green wastewater and recycling resources, which comprises the following steps: adjusting pH to be between 3.5 and 7 by adding diluted alkaline into the malachite green wastewater, filtering the malachite green wastewater to obtain filtrate and making the malachite green wastewater flow through an adsorption column or an absorption tower filled with activated carbon fibers, so that malachite green is absorbed onto the activated carbon fibers; directly discharging absorption effluent when the pH of the effluent is 6 to 7, and if the pH of the absorption effluent is lower than 6, rationally adding the diluted alkaline into the absorption effluent until the pH is adjusted to be between 6 and 7 and then discharging the absorption effluent; taking the solution of hydrochloric acid and ethanol as a desorbent and performing desorption and regeneration on the activated carbon fibers absorbing the malachite green; distilling a desorption solution containing high-concentration malachite green, recycling the desorbent and taking the desorbent as the desorbent of a next batch and separating malachite green solids out from residual liquid at the bottom of a kettle so as to recycle the malachite green; and taking the desorption solution containing low-concentration malachite green as a starting desorbent of the desorbent of the next batch. The malachite green wastewater treated by the method has the removing rate of the malachite green approximate to 100 percent, and desorption rate of the malachite green over 97 percent. In respect of the treatment on the malachite green wastewater and the purification of low-concentration malachite green water bodies, the method is of greater application value.

The invention relates to a malachite green collaurum detection card and production and application methods thereof, belonging to the technical field of immunology. The rapid diagnosis card comprises a case and a test strip; wherein the case is opened with a sample injecting hole and an observation window, the test strip is arranged in the case, the middle part of a PVC liner plate is superposed with a nitrocellulose membrane, the two ends are respectively superposed with an absorbent pad and a sample pad, the nitrocellulose membrane is overlapped and connected with the absorbent pad and the sample pad, a membrane containing malachite green monoclonal antibody immune collaurum compound is coated on a sample region, and a detection strip containing malachite green envelop antigen and a quality control strip containing goat anti mouse IgG are arranged on the nitrocellulose membrane. The invention is a rapid, accurate and sensitive diagnosis card, requires no special apparatus, has simple operation and can meet the detection requirements of food safety, fishery and detection mechanism.

The invention discloses a preparation method of a conductivity-controllable fly ash-based geopolymer semiconductor material. The preparation method comprises the following steps: putting fly ash, carbon black, sodium silicate nonahydrate and an aqueous solution of potassium hydroxide into a stirring device and mixing; and forming with a mould and curing to obtain the conductivity-controllable fly ash-based geopolymer semiconductor material, wherein the dosage of the sodium silicate nonahydrate, potassium hydroxide, carbon black and water accounts for 15%, 7%, 0.5-4.5% and 30-40% of the fly ash mass respectively; the stable conductivity of the prepared fly ash-based geopolymer semiconductor material can be controlled in a range from 0.00025 to 0.65 (S / m) in a curing age of 28 days; and when the prepared conductivity-controllable fly ash-based geopolymer semiconductor material is used as a novel photocatalyst for degrading malachite green organic dye, a change law that the conductivity of the material is directly proportional to the degradation rate of the dye is discovered. The preparation technology is simple, the cost is low, the discharge of three wastes is avoided in the preparation process, and the conductivity-controllable fly ash-based geopolymer semiconductor material can be used as a dye degrading high-activity catalyst.

The invention discloses a malachite chemiluminescence ELISA detection method and a kit. According to the invention, it is the first time to discuss chemiluminescence ELISA mechanism of malachite, a malachitechemiluminescence ELISA detection system and a detection method are successively established, and perfect combination of high sensitivity and high specificity is realized. The kit provided by the invention has high sensitivity, accuracy, precision and stability, is used to simplify operation steps and reaction time and reduce errors caused by complex operation, is very suitable for trace analysis and batch detection of malachite residues, and is of great practical application significance.

The invention discloses a Fe3O4 / SiO2 / Au magnetic particle, and a method for modifying the surface of the magnetic particle through utilizing cationic polyelectrolyte and anionic polyelectrolyte, wherein the method enables an anionic pigment of sunset yellow FCF and a cationic pigment of malachite green to be selectively gathered on the surface of a magnetic substrate. Raman characteristic peaks of the anionic and cationic pigments are observed, respectively, through a portable Raman spectrometer. It is proved through a series of experiments that a sample preparation method and a target object analysis method adopted in the invention are simple to operate, and selective detection of pigments is achieved.

This invention discloses Pantoea sp. M3 (CCTCC No.M207022) with the ability to degrade malachite green. Logarithmic phase Pantoea sp. M3 2-4 wt. % is added into sterile degradation medium containing 0.5-5 mg / L malachite green, and cultured at 20-30 deg.C for 5-10 days to degrade malachite green. The degradation medium with a pH value of 6.0-8.0 comprises: glucose 0.5-1 g / L, NaCl 0.01-0.1 g / L, MgSO4 0.01-0.1 g / L, NH4NO3 0.02-0.1 g / L, K2HPO4 0.01-0.1 g / L, KH2PO4 0.01-0.1 g / L, and yeast extractum 0.06-0.3 g / L. Pantoea sp. M3 can effectively remove residual malachite green and leucomalachite green in water body for fishery. The degradation rate of Pantoea sp. M3 for 0.5-2 mg / L malachite green is higher than 90%.

The invention discloses a preparation method of a silver phosphate composite photocatalyst for treating malachite green in water. The method comprises the following steps of: (1) performing ultrasonictreatment on multi-walled carbon nanotubes (MWCNTs) treated by HNO3 for 2-5 h; (2) dropwise adding an AgNO3 solution to a MWCNTs solution and performing stirring in the dark for 6-20 h; (3) adding Na2HPO4.12H2O dropwise to the above solution, performing stirring in the dark for 1 to 6 hours, and then performing vacuum drying; (4) uniformly mixing [(CH3)2CHO]4Ti, Sr(Ac)2 and Cr(NO3)3.9H2O with a molar ratio of 20:19:1 in an ethylene glycol solution, performing continuous stirring at 120-160 DEG C until the solution becomes a powder; (5) mixing the obtained powder with NaOH solution with a concentration of 2-6 mol / L under stirring for 20-60 min, placing the mixture in a hydrothermal reaction kettle, and performing a reaction at 180 DEG C for 30-40 h; and (6) performing ultrasonic treatmenton MWCNTs@Ag3PO4 for 10 to 60 min, adding a polyvinylpyrrolidone solution, performing stirring for 20-60 min, adding the ultrasonically-treated Cr:SrTiO3 dropwise to the above stirred solution, performing a reaction for 3-8 hours, and performing washing and drying, so as to obtain Ag3PO4@MWCNTs@Cr:SrTiO3. The material prepared by the preparation method of the invention has the advantages of friendliness to environment, economy, good visible light catalytic performance and the like.

The invention discloses a preparation method and application of a mercury ion detection probe. The method comprises the following steps of: 1, adding purified gold nanorods into a cetyl methyl ammonium bromide solution, stirring, then orderly adding an ascorbic acid solution, a silver nitrate solution and a sodium hydroxide solution, stirring, and centrifuging to get a solution A; 2, adding isothiocyano-malachite green Raman molecule into the solution A, eddying and mixing uniformly to get a mixed liquid B; 3, incubating the mixed liquid B in a dark condition, until realizing adsorption equilibrium, therewith, obtaining the final product, namely the mercury ion detection probe. The invention also relates to the application of the mercury ion detection probe, the mercury ion detection probe can be used for detecting the solution containing mercury ions. The probe prepared by the method disclosed by the invention is high in sensitivity, high in accuracy, quick in detection speed, simple to operate, low in cost and low in energy consumption, and can be widely used in the fields of environment detection, food safety, diseases detection, and so on.

The invention relates to a new strain golden Brevibacterium strain AN3 CGMCC No.2193, and application thereof in degrading triphenyl methane dyes and azo dyes such as malachite green. The golden Brevibacterium strain AN3 is obtained by separating and purifying activated sludge in an anaerobic baffled reactor system used for treating printing and dyeing wastewater by an research institute in Guangzhou, can degrade the triphenyl methane dyes in a mode of benzene ring detoxication under a facultative anaerobic condition that the temperature is between 25 and 37 DEG C and the pH value is between 6.0 and 8.5, and has significant capability on degrading the prior triphenyl methane dyes and azo dyes such as the malachite green which bring about serious pollution to aquatic products and are used in the form of a fungicide, wherein the removal rate of the golden Brevibacterium strain to the malachite green with the concentration of 50 milligrams per liter is 61.4 percent in 8 hours and is 97.3 percent in 16 hours, and the highest tolerable concentration thereof is 500 milligrams per liter, so that the strain golden Brevibacterium strain can effectively solve the problems of pollution and purification caused by the malachite green in an aquaculture environment.

The invention discloses a lead acid battery material. According to the invention, an electromagnetic resonant analyzer is used to analyze characteristics of various natural minerals and metal materials. In particular, coal, charcoal, malachite, zeolite, feldspar, Mengmoli pumice stone, limestone, gypsum, powdered steatile, silver or their combined materials are crushed into powders, the powders are added into granite and tourmalinite powders, and all the powders are mixed together to prepare lead plates, ceramic and partition plates for lead acid battery so as to generate highfield-like vibration frequency shared by active molecules. Thereby, the molecular structure of an electrolyte is changed so as to accelerate ion exchange speed, accelerate charging, increase transformation rate, minimize accumulation of sulfuric acid crystals, reduce corrosion speed of an anode checker and prolong battery life.

The invention belongs to the field of high sensitivity electrochemiluminescence detection, and in particular relates to a method for detecting malachite green through electrochemiluminescence. The method comprises the following steps of: dissolving a certain amount of bipyridyl ruthenium complex and malachite green in phosphoric acid or boric acid buffer solution, and scanning in a potential range of 0 to 1.8V by an electrochemical method; and detecting the malachite green by using mononuclear terpyridyl ruthenium and binuclear terpyridyl ruthenium respectively, wherein the detection range of mononuclear ruthenium is 1*10<-8> to 1*10<-5>mol / L, and the lowest detection limit is 1*10<-8>mol / L; and the detection range of binuclear ruthenium is 1*10<-12> to 1*10<-8>mol / L, and the lowest detection limit is 1*10<-12>mol / L. By the method, the malachite green can be more effectively detected, the detection limit is lower, the detection linear range is wider, sensitivity and repeatability are high, and the test can be finished instantaneously.

The invention discloses a treatment method of malachite green dye wastewater, and aims to solve the problems in an existing treatment method of malachite green dye wastewater, such as high cost, long period, poor operation stability and likelihood of causing secondary pollution. The method comprises the following steps: (1) preparation of a mixed adsorbent: mixing steel slag and coke in a mass ratio of 1:0.8-1.5; (2) oscillation: adding an adsorbent 0.8-2 wt% of the wastewater into the malachite green dye wastewater, and oscillating for 0.5-30 h; and (3) solid-liquid separation: separating steel slag in the wastewater to obtain treated water. The invention has the characteristics of simple method, low production cost, high efficiency and short treatment time; while, the used steel slag can be treated by microwave for reuse, so as to avoid secondary pollution.

The invention discloses a Bi2S3-BiFeO3 composite visible-light-driven photocatalyst prepared through in-situ growth with a chemical corrosion method and efficient in visible light response and an application of the Bi2S3-BiFeO3 composite visible-light-driven photocatalyst. According to the method, a sol-gel method and the chemical corrosion method are adopted synchronously. The method comprises the following steps: BiFeO3 is prepared firstly with the sol-gel method, then L-cysteine is taken as a sulfur source, and the Bi2S3-BiFeO3 composite visible-light-driven photocatalyst having different proportions of Bi2S3 to BiFeO3 are synthesized on the BiFeO3 surface through in-situ growth with the chemical corrosion method. A xenon lamp is taken as the light source, malachite green is taken as an organic pollutant degradation model, and a composite material and a malachite green solution are sufficiently mixed for photocatalytic degradation and simulation of malachite green organic pollutants in a water environment. With adoption of the method, more than 98% of malachite green in water is removed within 1 hour, the concentration is 20 mg / L, the photocatalyst has stable performance and a nanoflower structure, and the Bi2S3-BiFeO3 visible-light-driven photocatalyst which is synthesized successfully for the first time has great significance and broad development prospects in sewage treatment.

The invention discloses a normal-temperature desulfurizer and a preparation method thereof. The desulfurizer comprises a carrier, a binder, basic zinc carbonate, Cu2(OH)2CO3 and similar malachite. The preparation method comprises the following steps of: (a) configuring a soluble zinc salt solution and a soluble copper salt solution; (b) controlling a PH value of a reaction end solution with a solid carbonate and carrier mixture to obtain sediments; (c) filtering and washing a filter cake obtained in the step (b) in water; and (d) drying the filter cake obtained in the step (c), and after uniformly mixing the dried filter cake with the binder according to the weight ratio of 95:5 to 99:1, extruding, moulding and drying to obtain the desulfurizer. The desulfurizer is suitable for removing hydrogen sulfide at normal temperature and can reach 40 percent of penetrating sulfur capacity at normal temperature and normal pressure.

The invention provides an aptamer electrochemistry biosensor capable of detecting malachite green and a preparation method thereof and belongs to the technical field of biosensors. A single-chain DNA aptamer is adopted as a recognition element and contains 38 basic groups, and methylene blue which is modified with -SH-(CH2)6- at the 5' end and modified with an electroactive substance at 3' end is utilized as a beacon molecule. The aptamer is fixed to the surface of a gold electrode through an Au-S bond; methylene blue is utilized as an electrochemical response signal, and the diamond green and the aptamer interact to change the spatial configuration of the aptamer, so that electrochemical signals become stronger, and thus rapid detection on diamond green is realized. The aptamer electrochemistry biosensor has the advantages that the aptamer is economical in price and easy to mark; the sensor is simple in preparation process and detection method, and can be applied to accurately and rapidly detecting the malachite green with high sensitivity on site in water.

The present invention is one kind of immobilized microbial preparation and the method of degrading malachite green pollutant in aquiculture water therewith. The immobilized microbial preparation is prepared with Pantoea sp. M3 strain as effective component and through the following steps: 1. inoculating M3 seed to enrichment culture medium, culturing at 30-37 deg.c and 150 rpm for 18-30 hr, centrifuging, and washing with phosphate buffer to obtain concentrated bacterial suspension; 2. dissolving sodium alginate in water through heating, cooling to room temperature, mixing with the concentrated bacterial suspension; 3. dropping the mixture into 5 % concentration CaCl2 solution to form calcium alginate pellets and cross-linking to fix at 4-8 deg.c; and 4. washing the calcium alginate pellets with phosphate buffer. The present invention has high malachite green pollutant eliminating rate.

The invention relates to a transmutation glaze comprising the following components by the weight fraction: 49-53 parts of opal, 18-21 parts of purple knar clay, 14-17 parts of apatite, 3-5 parts of talc, 9-11 parts of feldspar, 0.6-1.2 parts of lasurite, 4-6 parts of iron oxide red, 14-16 parts of rutile, 10-15 parts of malachite, 3-5 parts of cobalt oxide, and 1-2 parts of zinc oxide. A prepared transmutation ceramic product has a dark red glaze surface, has a blue water drop-shaped pattern in a distribution manner, and is bright in color and luster, stronger in decorative effect, and good in thermal stability.

The invention discloses an immune colloidal gold test stripe for detecting leucomalachite green in aquatic products and a preparation method; the colloidal gold test stripe provided by the invention detects leucomalachite green with a direct competition method, and comprises a sample pad, a colloidal gold combined pad, a nitrocellulose membrane (NC), a water adsorption pad and a PVC back lining, one end of the PVC back lining is sequentially adhered with the sample pad and the colloidal gold combined pad, and the middle thereof is adhered with the NC layer, and the other end thereof is sequentially adhered with water adsorption pad; the immune colloidal gold test stripe has the characteristics that colloidal gold combined pad is enveloped with colloidal gold marker which is monoclonal antibody of the specificity of the leucomalachite green, and the NC is adsorbed with leucomalachite green-OVA and sheep anti-mouse IgG. The invention has high detection sensitivity and low price and is simple and rapid, the whole reaction only needs 30 minutes; the immune colloidal gold test stripe can be used for large-batch screening of samples and can rapidly detect the residual leucomalachite green in the aquatic products at site.