Method for treating malachite green dye waste water

A technology for dye wastewater and malachite green, applied in the field of applied microorganisms and environmental engineering, can solve the problems of complex preparation process, secondary environmental pollution, low immobilization efficiency, etc., and achieves simple operation, small environmental impact and good stability. Effect

Inactive Publication Date: 2011-02-16
NORTHEAST DIANLI UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned carriers have many problems, such as complicated preparation process, low immobilization

Method used

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  • Method for treating malachite green dye waste water
  • Method for treating malachite green dye waste water
  • Method for treating malachite green dye waste water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Preparation of white rot fungus spore liquid:

[0025] Pick a small amount of bacteria from the slant of the white-rot fungus with the preserved strain number CGMCC NO: 5.776, and inoculate it on the solid medium by streaking. The percentages are: Potato Dextrose Agar 3.8%; KH 2 PO 4 0.3%; MgSO 4 ·7H 2 O 0.15%, after 5 days of cultivation at 30°C, the spore hyphae of the white rot fungus amplified in large quantities, inject a small amount of sterile water into the plate, scrape off the spore powder with the push rod method, and filter the mycelium through 4 layers of lens-cleaning paper. The obtained milky white spore suspension is the white rot fungus spore liquid, which is stored in a refrigerator at 4°C for subsequent use;

[0026] The white rot fungus used in the above steps was purchased from China General Microorganism Culture Collection and Management Center, and the strain number is CGMCCNO: 5.776.

Embodiment 2

[0028] Preparation of bacterial cellulose membrane:

[0029] The bacterial strain number that is preserved on the slant is CGMCC NO:1.1812 Acetobacter xylinum Gluconacetobacter xylinum is inserted in 50mL seed culture medium, and the percentage that each component consumption accounts for the quality consumption of seed culture medium in the described seed culture medium is respectively: Glucose 2%; peptone 0.5%; yeast extract 0.3%; Na 2 HPO 4 12H 2 O0.2%; KH 2 PO 4 0.1%; MgSO 4 ·7H 2 O 0.025%; citric acid 0.2%; pH5.8; cultivated on an air bath constant temperature shaker at 160rpm for 2 days to obtain an activated Acetobacter xylinum culture solution, take 10mL of this Acetobacter xylinum culture solution and insert it into 125mL fermentation medium , the percentages of the amount of each component in the fermentation medium to the quality of the fermentation medium are as follows: glucose 2%; peptone 0.5%; yeast extract 0.3%; Na 2 HPO 4 12H 2 O0.2%; KH 2 PO 4 0.1%...

Embodiment 3

[0032] Modification of bacterial cellulose membrane:

[0033] Put 40g of bacterial cellulose membrane into 100mL of 1mol / L sodium hydroxide solution, then add 20mL of epichlorohydrin, react in a constant temperature water bath at 60°C for 30min, take out the membrane and wash it with acetone to remove the residues on the membrane. Small molecular organic matter; after washing with water, add 5.5mL of ethylenediamine and 49.5mL of distilled water, shake fully, heat in a constant temperature water bath at 80°C for 30min, then take out the membrane and wash it with distilled water until it is neutral, and then use 1% weight After washing 3 times with hydrochloric acid with a certain percentage content, wash with distilled water until neutral, then rinse with acetone, and then vacuum-dry to constant weight. At this time, the flexibility of the film becomes stronger, and the surface is light yellow, smooth and shiny. It is a modified bacterial cellulose membrane;

[0034] Immobili...

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Abstract

The invention discloses a method for treating malachite green dye waste water, which comprises the following steps of: preparing a bacterial cellulose membrane; modifying the bacterial cellulose membrane; immobilizing white rot fungi by using the modified bacterial cellulose membrane; adding the obtained immobilized white rot fungi modified bacterial cellulose membrane into 50 mL of the malachite green dye waste water; dissolving 20 to 40 mg of malachite green into 1 L of white rot fungi liquid restrictive culture medium; treating the waste water on a gas bath constant-temperature shaking table at a speed of 120 rpm for 5 days at 28 DEG C, and measuring the chroma of the waste water at intervals of 24 hours; 5 days later, discarding the treated waste water, and retaining the immobilized fungi; adding 50 mL of newly-prepared dye waste water with the same concentration, treating the waste water under the same condition, and measuring the chroma of the waste water at intervals of 24 hours; and taking 5 days as a cycle, and repeating the steps for 5 times, wherein the removal rate of the chroma of the waste water is always over 86 percent. In the method, the referred bacillus xylinus and the white rot fungi are purchased from the China General Microbiological Culture Collection Center.

Description

technical field [0001] The invention belongs to the technical field of applied microorganisms and environmental engineering, and relates to a method for treating dye wastewater by using a novel immobilized carrier to immobilize microorganisms. In particular, it relates to the treatment of malachite green dye wastewater by using the modified bacterial cellulose membrane of immobilized white rot fungus. Background technique [0002] Malachite green is a toxic triphenylmethane chemical. After the condensation of benzaldehyde and N,N-dimethylaniline in hydrochloric acid or sulfuric acid to form a recessive base of tetramethyl-substituted diaminotriphenylmethane, It is made by oxidation of lead dioxide in acid medium. At present, the treatment of malachite green dye wastewater mainly adopts photocatalytic oxidation method, microwave method, iron oxide battery method and diatomite porous ceramic method, etc., but these methods have high cost, long cycle, poor operation stability,...

Claims

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Application Information

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IPC IPC(8): C02F3/34C02F103/36
Inventor 乔楠于大禹张金榜关晓辉
Owner NORTHEAST DIANLI UNIVERSITY
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