562 results about "White rot fungus" patented technology

The invention relates to a bio-protein feed prepared by adopting a cellulose raw material, and the bio-protein feed provided by the invention comprises an organic material and complex bacteria, wherein the weight of the complex bacteria is 1-10% of that of the organic material; and the organic material comprises the cellulose raw material, rapeseed meal and bran; the cellulose raw material is one or more of corn stalks, corn cobs, straw, manioc waste and fruit residue; and the complex bacteria comprises bacillus subtilis, microzyme, lactobacillus and cellulose decomposition bacteria, and the cellulose decomposition bacteria is one or more of trichoderma harzianum and white-rot fungi. In the bio-protein feed, the content of probiotics is more than 108cfu/g, the bio-protein feed contains a variety of prolease, cellulase, amylase and the like, the preparation process is simple, and the fermentation period is short. The invention further discloses a preparation method of the feed.

A method for treatment of liquid waste is disclosed that includes the steps of (a) submitting the liquid waste to a pretreatment and (b) submitting the pretreated liquid waste to the action of fungi or active agents thereof. In particular, the described process is useful for the effective decoloration and detoxification of dye-containing liquid wastes using white rot fungi or active agents thereof.

A marine-derived Bacillus velezensis (Bacillus velezensis) BMF 03 having a deposit number of CCTCC NO: 2017374 is disclosed. BMF 03 strain and its aseptic fermentation broth have inhibitory effect onplant pathogenic fungi, including apple rot fungi, grape white rot fungi, mango anthracnose fungi and so on. The aseptic fermentation broth of BMF 03 strain can be used as an active ingredient to prepare an antibacterial drug, and the bacteria is selected from the group consisting of Keratinobacteria, Bacillus subtilis and Ralstonia solanacearum. The invention also discloses a fermentation methodof aseptic fermentation broth of BMF 03. The invention adopts a plate confrontation method and an Oxford cup method to screen a new marine bacterial strain BMF-03 with strong bacteriostatic effect andwide bacteriostatic spectrum, and adopts strain morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis to determine the species relationship of excellent resistant strains. At that same time, the bacteriostatic substance produce by the strain, the sporulation fermentation medium and the shake flask fermentation conditions were also study, and the promoting effect of the strain fermentation broth and the solid fermentation product on the growth of the cucumber seedling was clarified, which laid a foundation for the application of the strain.

A method of amplification culture of microorganism for waste water treatment. The waste water comprises heterocyclic compounds, polycyclic aromatic hydrocarbons, cellulose and/or lignin. The method comprises the following steps: adding waste water containing heterocyclic compounds, polycyclic aromatic hydrocarbons, cellulose and/or lignin into an acclimation reactor; adding a nutritional agent, acomplex bacterial agent, an enzyme preparation, a citric acid and riboflavin on a basis of concentrations in the waste water; performing aeration of the waste water, adjusting the liquid level and temperature of the acclimation reactor, holding for a period of time to allow the microorganism in the complex bacterial agent to reach a logarithmic growth phase, wherein on a basis of concentrations in the waste water, the complex bacterial agent comprises pseudomonas spp. microorganism, bacillus sp. microorganism, and white rot fungi, and the enzyme preparation comprises protease, amylase, cellulose and lipase. The invention also provides a method of waste water treatment by using the microorganism to be amplified and cultured.

The invention relates to Trichoderma harzianum Rifai WRF-2 and use thereof. The bacterium strain was preserved in China General Microbiological Culture Collection Center on January 21st, 2009, with a preservation number of CGMCC No.2870. The bacterium belongs to deuteromycetes and is a lower fungus that is short in culture period and quick in growing and propagating speed and produces a large amount of uniform culture liquid for lignin degrading enzyme-generating bacteria in a short time compared with a major part of basidiomycetes to which whiterot fungi belong. The invention also provides a method for culturing and producing lignin peroxydase (Lip) and manganese peroxidase (MnP) by the fermentation of the bacterial strain in a liquid enzyme-generating culture medium, which can produce high-acitivity LiP and MnP in a short time, and is simple in fermentation process, stable, low in cost, and high in yield.

The invention provides a pretreatment method for obtaining ligno-cellulose materials of a transformable substrate required in the production of bio-refinery, bio-energy, biological medicine, food processing, light chemical products, biological feedstuff and fertilizers, which adopts baisdiomycete strains or flora that can selectively destroy the structure of ligno-cellulose to carry out continuous pretreatment of solid fermentation to various ligno-cellulose materials in an open condition. The transformable substrate can be obtained directly and simply by extraction after the pretreatment or obtained by combining the treatment of the physical and chemical biomass white-rot fungus degradation system with the complex enzymatic method. The method of the invention puts biological degradation, modification and biomass transformation in leading position, partially combines with the physical and chemical enzymatic treatment process technology and has the advantages of strong operability, wide application scope, and the like.

The invention discloses a method for treating malachite green dye waste water, which comprises the following steps of: preparing a bacterial cellulose membrane; modifying the bacterial cellulose membrane; immobilizing white rot fungi by using the modified bacterial cellulose membrane; adding the obtained immobilized white rot fungi modified bacterial cellulose membrane into 50 mL of the malachite green dye waste water; dissolving 20 to 40 mg of malachite green into 1 L of white rot fungi liquid restrictive culture medium; treating the waste water on a gas bath constant-temperature shaking table at a speed of 120 rpm for 5 days at 28 DEG C, and measuring the chroma of the waste water at intervals of 24 hours; 5 days later, discarding the treated waste water, and retaining the immobilized fungi; adding 50 mL of newly-prepared dye waste water with the same concentration, treating the waste water under the same condition, and measuring the chroma of the waste water at intervals of 24 hours; and taking 5 days as a cycle, and repeating the steps for 5 times, wherein the removal rate of the chroma of the waste water is always over 86 percent. In the method, the referred bacillus xylinus and the white rot fungi are purchased from the China General Microbiological Culture Collection Center.

The invention relates to a straw feed fermented by white rot fungus and a preparation method thereof, and strain is the combination of white rot fungus Tf1 (pleurotus sajor-caju) and JG1 (pleurotus cornucopiae) which are screened in an oriented way, so that solid fermentation fungicide is cultured and prepared by three-stage propagation. Straw is pretreated by quicklime instead of being processed by high temperature sterilization without being washed by water or neutralized by acid. The obtained straw is accessed with white rot fungus composite fungicide to be fermented for 15-20 days, thus being taken as a first step of fermentation for degrading lignin in the straw and improving the straw digestion utilization rate of ruminant. After that, the fermented straw is accessed with yeast to be fermented for 3-4 days, thus being taken as a second step of fermentation for improving the crude protein content of the straw and further enhancing the nutritional value of the straw feed. After the two steps of fermentation, the crude fiber content of the straw is reduced by over 25%, the dry matter rumen (nylon bag technique) digestion rate is improved by over 20%, the crude protein content is increased by more than 50%, and the straw feed has no safety risk when being fed for animals. The invention has the characteristics of no energy consumption, no pollution, low cost, easy operation, high feeding value of the straw, and being suitable for local production of villages in China.

The invention discloses a lignin degradation solution and a preparation method thereof as well as a method for degrading lignin by using the lignin degradation solution, and belongs to biochemical and bio-refinery methods to solve the problem that the efficient biodegradation of lignin is difficult to realize by adopting single ligninase of laccase at present. The lignin degradation solution is prepared by the following steps: dissolving laccase and manganese peroxidase into an acetic acid-sodium acetate buffer solution of which the pH value is 4-6 in an enzyme load ratio of (10:1)-(1:5) to ensure that the enzyme loads of the laccase and manganese peroxidase are respectively 1U/ml-50U/ml and 1U/ml-50U/ml; and then adding 1-10mM of MnSO4 and 0.1-1mM of H2O2, wherein the laccase and manganese peroxidase are obtained by fermenting white rot fungi to obtain extracellular crude enzyme liquid and then performing separation and purification respectively. According to the lignin degradation solution and the methods disclosed by the invention, the synergistic oxidative degradation of macromolecular lignin with rich structural diversity is realized, and compared with a degradation reaction system with single ligninase, the degradation rate of the macromolecules of the lignin can each 30-50%, the degradation conversion efficiency is significantly improved, and the degradation solution and the methods can be applied to the fields of bio-refinery of lignocelluloses, biological pulping or environmental treatment and the like.

The invention discloses a micro-ecological flora of crop root system probiotics and an application thereof. The micro-ecological flora comprises a leading flora, a balance flora, a basic flora, a secondary-function flora and a special-function flora, wherein the leading flora comprises 25-35 parts of nitrogen-fixing bacteria by mass; the balance flora comprises 17-23 parts of phosphate solubilizing bacteria and 17-23 parts of potassium solubilizing bacteria by mass; the basic flora comprises 2-7 parts of yeast, 2-7 parts of rhizobia and 2-7 parts of photosynthetic bacteria by mass; the secondary-function flora comprises 2-7 parts of bacillus and 2-7 parts of actinomycetes by mass; the special-function flora comprises 2-4 parts of white rot fungi and 1-3 parts of pseudomonas. In the micro-ecological flora disclosed by the invention, the strain stability is good, functional microorganisms can be effectively planted in soil, and the functions of improving the crop yield and quality, improving the soil, detoxifying heavy metals and resisting diseases and pests can be realized.

The invention relates to a microbial fermentation technology and particularly relates to a microbial agent for high-efficiency straw fermentation. The microbial agent comprises white-rot fungus, lactobacillus acidophilus, trichoderma viride, bacillus subtilis and candida utilis, and is prepared from such five microbial bacteria as the white-rot fungus, the lactobacillus acidophilus, the trichoderma viride, the bacillus subtilis and the candida utilis. the microbial agent can be used for the high-efficiency fermentation of straw feed so that the straw feed contains a large amount of living biological cells, decomposition enzymes and other active components and the utilization rate of the straw feed is improved effectively. The microbial agent has the practical significance of solving the problem of environment pollution, has the actual application value of increasing the content of protein in the straw feed and the nutritional value of the straw feed and creates a good precondition for the utilization of straw as a resource.

The invention discloses a wheat straw decomposition agent and a method for preparing the same. The wheat straw decomposition agent and the method have the advantage that bacillus subtilis, trichoderma viride, aspergillus niger, white rot fungi and candida are used as production strains, are free of antagonism and easy to cultivate and are nontoxic and harmless, and excellent wheat straw degradation effects can be realized; the method for preparing the wheat straw decomposition agent is simple, the strains are easily available and are convenient to store and low in cost, and obvious decomposition effects can be realized; the yield of crops can be greatly increased by decomposed wheat straw organic fertilizers, and the quality of the crops can be greatly improved by the decomposed wheat straw organic fertilizers.

The invention discloses a grass fiber bio-separation compound preparation and an application method thereof. The grass fiber bio-separation compound preparation comprises bacteria components and enzyme components, wherein the bacteria components comprise white rot fungi and zymogeneous bacteria, and the enzyme components comprise cellulose, xylanase, laccase, protease, thermostable amylase, ligninase and pectinase. According to the grass fiber bio-separation compound preparation, straw serving as the raw material is treated with a biological method, a biological cycle liquid and a waste cooking liquid produced in the treatment process can be recycled without drainage, and lignin can be extracted; and besides, according to the method, straw does not require cutting and dust removal, and the cooking does not require high temperature and high pressure, so that a large quantity of manpower and electric charges is omitted, and the preparation and the method are suitable for a plurality of raw materials such as wheat straw, straw, cotton stalks, reeds and the like, can be used for producing a paddle board, molded pulp, various types packaging paper, office paper and household paper and have the advantages of low cost and environment protection.

The invention discloses compound bactericide for treating sewage and a sewage treatment method thereof, and belongs to the technical field of sewage treatment. The compound bactericide comprises the following active ingredients from following strains: rhodopseudomonas, anaerobic ammonium oxidation bacteria, candida utilis, thiobacillus denitrificans, white-rot fungi, lactobacillus plantarum, bifidobacterium, lactobacillus acidophilus, green nonsulfur bacteria, phosphorus-accumulating bacteria, aspergillus oryzae, nitrosomas, streptomyces, bacillus subtilis and propionibacterium acidipropionici. The invention also provides the method using the compound bactericide to treat the sewage. The compound bactericide and the sewage treatment method have the advantages that the effect of treating sewage is better, the technology is simple, the implementing is easy, the operability is strong, the pollution to environment is avoided, the industrial requirements are met, and the application prospect is better.

The invention discloses a modified rice straw stalk and a preparation method and an application thereof. The modified rice straw stalk is obtained by the steps of carrying out solid-state fermentation of rice straw stalk, a white-rot fungus spore suspension and a trace element liquid, then mixing with oxalic acid, and carrying out ultrasonic treatment and heating reaction. The preparation method includes the steps of solid-state fermentation and chemical treatment. The modified rice straw has high specific surface area and specific pore area, contains more -COO<-> groups, and can be applied to treatment of wastewater containing cadmium; an application method includes the following steps of mixing the modified rice straw stalk with the wastewater containing cadmium, and carrying out oscillation adsorption, wherein the mass volume ratio of the modified rice straw stalk to the wastewater containing cadmium is (5 g-6 g) to (1 L). The modified rice straw stalk has the advantages of high treatment efficiency, good treatment effect, environmental protection and no secondary pollution.

The invention discloses a method for removing 2,4-dichlorophenol in water by using polyurethane sponge fixed white rot fungi, belonging to the field of environmental microbiology. The method comprises the following steps: preprocessing a carrier, preparing fixed white rot fungi and removing 2, 4-dichlorophenol in water. The carrier has the characteristics of porosity, hydrophily and good adsorbability, enjoys stable chemical property and hydrolytic resistance, high organism bearing capacity and mass transfer rate and is not soluble in acid/alkaline water solution; while the fixed carrier can effectively absorb and fix white rot fungi, improve capacity of fixed white rot fungi in removing 2,4-dichlorophenol in water and leads the fixed white rot fungi to be easily separated from the product, thus being applicable to execute biological reaction processing on multiple sewages.

The invention relates to a method for preparing activated carbon fibers by the use of cotton linters, comprising the following steps of: placing cotton linters into a fermentation cylinder, inoculating turkey-tail or white rot fungus, performing a fermentation treatment, followed by washing again and drying; placing KOH or NaOH into a reaction vessel, heating until KOH or NaOH is melted; adding the treated cotton linters to the molten NaOH or KOH with the mass ratio of cotton linters to KOH or NaOH being 1:2-4, heating the mixture to the temperature of 600-800 DEG C, and performing a carbonization-activation treatment for 1-1.5 hours. The specific surface of the obtained cotton linter activated carbon fibers is over 2000m<2>/g. The technology provided by the invention is simple and is used to accomplish waste utilization. The prepared activated carbon fibers have large specific surface, strong sorption as well as excellent performance, and can effectively remove harmful pollutants.

The invention discloses antibacterial packaging paper. A preparation process comprises: preparation of packaging paper, preparation of a waterproof agent coating, and preparation of an antibacterial agent coating. According to the antibacterial packaging paper disclosed by the invention, the processing raw materials are bamboos and waste timbers. As bamboo fiber is naturally antibacterial, timbers are replaced by bamboos, so that cutting of trees is avoided, the environment is protected, the ecological balance is maintained, waste is turned into wealth as waste timbers are used, and the cost is saved; the bamboo fiber and wood fiber are matched in use, so that the strength and flexibility of paper are remarkably improved and the packaging paper is wide in using range; the waterproof layer and the antibacterial layer are natural materials which do not harm human body and environment and play natural roles of preventing water and resisting bacteria; in a delignification process, the raw materials are pre-treated by using white rot fungi first, so that the time and dosage for delignification by hydrogen peroxide are reduced; hydrogen peroxide further can disinfect and bleach the packaging paper, is safe and non-toxic, and does not cause the environmental pollution, thereby killing two birds with one stone and saving the cost.

The invention discloses a white fungus and coix seed cake which is prepared by the following raw materials in parts by weight: 30-40 parts of wheat flour, 10-20 parts of maize powder, 5-8 parts of fried coix seed powder, 6-8 parts of white fungus powder, 30-40 parts of egg, 30-40 parts of white granulated sugar, 5-10 parts of olive oil, 15-20 parts of cream, 1-2 parts of baking powder, 10-20 parts of coix seed composite yoghourt, 5-8 parts of mixed fruit and vegetable juice and a proper amount of water. The white fungus and coix seed cake is unique in mouth feel, high in resilience due to colloids in the white fungus and rich in nutrition due to the mixed fruit and vegetable juice; and the coix seed composite yoghourt is rich in nutrition and good in taste.

The invention discloses a method for treating dye waste water by enzyme production through mixed biomass fermenting. In the method, white rot fungi which can generate a lignin degrading enzyme system are used as a fermenting enzyme-production strain; a solid and liquid culture method is adopted, a cottonseed hull and/or paddy straw are/is used as a fermentation substrate, and lignin degrading enzyme is induced to obtain an optimal lignin degrading enzyme system; and finally, crude enzyme liquid is extracted, separated and prepared and can be used for decoloring treatment of waste water of commonly-used synthetic dye, such as Congo red, phenol red, bromophenol blue, crystal violet, malachite green and the like so as to obtain the optimal decoloring effect of dye sewage. The method is simple and is easy to operate. The enzyme system induced by the method disclosed by the invention has high enzymolysis efficiency, and the optimal decoloring effect can be achieved by adopting the enzyme system to treat the dye waste water.

The invention discloses an integration processing method of a high-efficient corn straw biological feed, and belongs to the field of biological feeds. Corn straw is subjected to three-step fermentation, so that a superior microorganism coarse feed for ruminant animals, with high efficiency and low cost, is produced in a large-scale manner. The integration processing method comprises the following steps of firstly, based on the corn straw, performing high-efficient pure solid cultivation on a white rot fungus enzyme preparation, then converting the cultivated white rot fungus enzyme preparation in large inoculum amount to a first-stage fermentation medium of the corn straw, and besides, inoculating serial lignocelluloses degradation bacteria and candida utilis bacteria for synergistic rapid degradation and transformation; then adding bacillus and saccharomyces cerevisiae for second-stage fermentation; and using lactic acid bacteria for third-stage anaerobic fermentation so as to obtain finished products. The raw materials used by the integration processing method do not need to be sterilized, the finished products do not need to be dried, lysozymes are enriched, the cost is low, the lignin is notably reduced, and genuine protein is increased to 12% or above. The high-efficient corn straw biological feed can be used as a superior coarse feed and a protein grain feed for cattle and sheep, and the additive quantity can reach 60% or above; the additive quantity can also be about 10%, so that the high-efficient corn straw biological feed is used for simple stomach animals. The technique is high in scalability and integrity, and the economic and social benefits are notable.