Trichoderma harzianum strain and use thereof

Abstract

The invention relates to Trichoderma harzianum Rifai WRF-2 and use thereof. The bacterium strain was preserved in China General Microbiological Culture Collection Center on January 21st, 2009, with a preservation number of CGMCC No.2870. The bacterium belongs to deuteromycetes and is a lower fungus that is short in culture period and quick in growing and propagating speed and produces a large amount of uniform culture liquid for lignin degrading enzyme-generating bacteria in a short time compared with a major part of basidiomycetes to which whiterot fungi belong. The invention also provides a method for culturing and producing lignin peroxydase (Lip) and manganese peroxidase (MnP) by the fermentation of the bacterial strain in a liquid enzyme-generating culture medium, which can produce high-acitivity LiP and MnP in a short time, and is simple in fermentation process, stable, low in cost, and high in yield.

Description

technical field [0001] The invention relates to the field of microorganisms and applications thereof, in particular to a Trichoderma harzianum strain and applications thereof. Background technique [0002] Lignin is an important part of plant cell walls. Lignin accumulates in large quantities on the earth every year through plant photosynthesis. In nature, lignin is rich in content and is a natural polymer compound second only to cellulose. It is estimated that the global annual yields about 6 x 10 14 t lignin. At the same time, lignin is also degraded by microorganisms, participates in the carbon cycle on the earth, and plays an important role in it. [0003] Lignin is the product of plant tissue evolution, which endows plants with the ability to protect themselves and resist the decay of microorganisms. It is one of the most difficult biodegradable substances in natural organic matter. In nature, the degradation of lignin is a relatively slow process, which is the rate-...

AI Technical Summary

Problems solved by technology

[0004] In the traditional papermaking industry, lignin has always been separated and discarded as an obstacle to obtain papermaking fibers. Because the traditional method of separating lignin is carried out at high temperature with chemical reagents such as acid and alkali, resulting in The high cost and environmental pollution problems are the problems that have plagued the paper industry

[0007] In recent decades, some research progress has been made in ligninase and lignin biodegradation, but due to the high production cost of ligninase, it has been difficult to realize industrialization

One of the main reasons for this is that most of the white rot fungi known and used in research are fungal basidiomycetes, which grow slowly, and most of them cannot form asexual spores, and can only reproduce by hyphae, and some can produce The ability of asexual spores to produce spores is also very low. Therefore, it is difficult to expand the cultivation in production, the cultivation period is long, and it is difficult to cultivate a large amount of uniform ligninase-producing bacteria culture solution in a short period of time.

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