46results about How to "The fermentation process is simple" patented technology

The invention discloses a multifunctional peoriae paenibacillus and application thereof, and relates to the technical field of microorganisms, and the key point of the technical scheme is as follows: Paenibacillus peoriae S6330 is separated from rhizosphere soil; the peoriae paenibacillus has a good inhibition effect on pathogenic bacteria, pathogenic fungi and oomycetes; meanwhile, the peoriae paenibacillus has the functions of dissolving potassium, producing protease, producing amylase, producing cellulase, producing siderophore and the like, has a growth promoting effect on various crops such as wheat, corn, watermelons, small oilseed rapes, tobaccos and the like, and can remarkably improve indexes such as chlorophyll content, plant height, fresh weight, overground part weight, underground part weight and the like of plants; in addition, the peoriae paenibacillus can be used for effectively inhibiting the tobacco bacterial wilt; the peoriae paenibacillus has no environmental pollution problem, is simple in culture condition, and has wide disease prevention and growth promotion application prospects in agricultural production.

The utility model provides a novel perishable garbage biological drying treatment system, a tower type treatment device comprises a barrel body, a feeding device, a material distributing device, a discharging system and an aeration oxygen supply dehydration system, the feeding device is arranged at the top of the barrel body and is communicated with a fermentation bin body in the barrel body, and the material distributing device is arranged on the top of the barrel body. The material distributing device is arranged in the top area of the fermentation bin body under an outlet of the feeding device, the discharging system is provided with a plurality of layered treatment units in the vertical direction of the barrel body, each layer of treatment unit is provided with supporting beams in the fermentation bin body at intervals, and the supporting beams are provided with a plurality of fermentation units in the circumferential direction of the barrel body. Materials in the bin body are fully mixed through layered staggered discharging and mixed feeding circulation, dehydration reduction serves as the purpose, a single high-temperature fermentation flora is cultured and domesticated, the fermentation process is simplified, the drying speed of perishable garbage is greatly increased, and the drying bin can be suitable for drying treatment of large-scale perishable garbage.

The invention discloses a white tea fermentation quality evaluation method, system and device based on image analysis, particularly relates to the technical field of food processing image analysis, and aims to solve the problem that the precipitation state of grease substances on the surfaces of white tea leaves cannot be effectively monitored and quantified in the prior art. The method comprises the following steps: acquiring image data of the whole surface of a leaf through multispectral image acquisition, identifying a reflective region by combining a local brightness enhancement algorithm and a brightness threshold segmentation algorithm, extracting reflection spectrum features, comparing the reflection spectrum features with a feature database, screening out a grease precipitation risk region, and further extracting texture features by using a gray-level co-occurrence matrix algorithm to obtain a grease precipitation risk region; according to the method, the roughness change is analyzed and evaluated in combination with the dynamic entropy, the abnormal index of the grease precipitation risk area is finally quantified, the fermentation quality of the white tea is accurately monitored and evaluated, and a scientific basis is provided for fermentation process optimization and quality improvement.

The invention belongs to the field of medicines, and particularly relates to a fermentation processing method for reducing toxicity of tripterygium wilfordii, which comprises the steps of tripterygium wilfordii matrix treatment, strain activation treatment and bidirectional solid fermentation. The method specifically comprises the following steps: crushing tripterygium wilfordii, and performing high-pressure steam sterilization to obtain a tripterygium And inoculating the fermentation seed liquid of the ganoderma lucidum strain into the tripterygium wilfordii matrix, and carrying out bidirectional solid fermentation. Starting from clinical application practice, the invention provides a more perfect, reasonable and systematic tripterygium wilfordii fermentation and toxicity reduction processing method. The method solves the problems that in the field of tripterygium wilfordii fermentation processing, ganoderma lucidum strains are not clear, most technical methods do not conform to actual clinical medication forms, the processing method has many uncertain factors, the non-druggability is increased after processing, and the like, and has popularization value.

The invention relates to the technical field of probiotics, in particular to pediococcus acidilactici ZKPa1222, astragalus mongholicus fermentation liquor of the pediococcus acidilactici ZKPa1222 and application of the pediococcus acidilactici ZKPa1222 in the aspect of improving immunity, the pediococcus acidilactici ZKPa1222 is preserved in China General Microbiological Culture Collection Center on May 13, 2024, the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No.30548, and the preservation number is CGMCC No.30548. The invention further discloses a preparation method of the pediococcus acidilactici ZKPa1222. According to the invention, the pediococcus acidilactici ZKPa1222 is used for directly fermenting the radix astragali extracting solution, so that the fermentation process is simplified, and the fermentation efficiency is improved.

The invention discloses a spatial multilayer ultrahigh-pressure box-type dehydration system and a dehydration method. The system comprises a main rack, a material distributing and feeding device, a material distributing / discharging device, filter cloth, a filter pressing chamber, a filter chamber moving device, a filter chamber lifting hydraulic machine, a filter pressing hydraulic machine, a hydraulic station and a controller, the material distributing and feeding device, the material distributing / discharging device, the filter chamber moving device, the filter chamber lifting hydraulic machine and the filter pressing hydraulic machine are connected to the main machine frame, the material distributing and feeding device is installed on the material distributing / discharging device, the material distributing / discharging device is installed on the filter pressing chamber, the filter cloth is wound on the material distributing / discharging device, the filter pressing chamber is installed on the filter chamber moving device, and the filter pressing hydraulic machine is installed on the filter chamber lifting hydraulic machine. The filter chamber lifting hydraulic machine is installed below the material distributing / discharging device and the filter chamber moving device, the filter chamber moving device, the filter chamber lifting hydraulic machine and the filter pressing hydraulic machine are connected with the hydraulic station, and the material distributing and feeding device, the material distributing / discharging device, the filter chamber moving device, the filter chamber lifting hydraulic machine, the filter pressing hydraulic machine and the hydraulic station are electrically connected with the controller.

The application discloses a kind of fermented feed fermented by three strains of probiotics of Bacillus YA ( Bacillus sp ), yeast Y ( Saccharomyces cerevisiae ) and capsular red bacteria R3 ( Rhodobacter capsulatus ), and preparation method and application in aquaculture thereof, and viable count is not less than 1×10 7 CFU / g.The fermented feed is replaced ordinary feed according to certain proportion, and feeding result shows that aquaculture water quality is significantly improved after adding fermented feed, and fish growth performance, digestive enzyme activity, immunity, and muscle quality are also obviously improved.Using the feed in suitable range can shorten growth cycle, reduce cost, improve aquaculture environment, reduce antibiotic use, and improve muscle quality of farmed animals, with good economic and ecological benefits, simple fermentation process, low cost, and suitable for industrial large-scale production.

The present application provides a wool amino acid water-soluble fertilizer and a preparation method thereof, which are applied in the field of fertilizer technology. A safe and efficient keratin-degrading strain is found to be used in combination with keratinase, thereby achieving a wool fermentation method with high wool degradation efficiency, simple and easy fermentation operation (temperature, pH value and other parameters are not demanding, and the culture medium components are simple), scalable production, rich amino acid types and high yield, and the fermentation liquid can directly produce wool amino acid liquid water-soluble fertilizer, which is applied in agricultural production.

This invention relates to a spatial multi-layer ultra-high pressure belt dewatering system and method, comprising a main frame, a feeding device, a feeding device, a filter belt, a filter belt traction machine, a filter press hydraulic press, a hydraulic station, and a controller. The feeding device, feeding device, filter belt traction machine, and filter press hydraulic press are connected and installed on the main frame; the output end of the feeding device is connected and installed above the feeding device. The dewatering method includes starting the equipment, feeding, filter press dewatering, and unloading. This invention offers higher dewatering efficiency and lower cost, making it a more effective organic waste dewatering device for reducing pollution and carbon emissions.

The utility model discloses a kind of citric acid fermentation fine filter devices, belong to citric acid production technical field.Device is composed of fermentation unit and fine filter unit: fermentation unit contains seed tank and fermentation tank, the feed inlet of seed tank is connected with syrup turbid liquid pipe, the feed inlet of fermentation tank is connected with syrup clear liquid pipe and syrup turbid liquid pipe, seed tank and fermentation tank are configured with steam, compressed air, defoaming agent, emptying and circulating water pipeline.Fine filter unit first removes most solid impurities with primary plate-and-frame filtration, then ceramic membrane fine filtration and anion and cation exchange impurity removal.Two-stage filter-pressing acid residue is dried after being thickened and then filter-pressing to be feed, and two-stage filtrate respectively enters subsequent process.The device can greatly reduce labor cost and failure rate, improve citric acid purity and yield, and meet the needs of large-scale, high-quality and green production.

The present invention provides a composite fermentation agent, a preparation method thereof and an application thereof in feed for ruminants. The composite fermentation agent comprises Lactobacillus plantarum, Candida utilis and Aspergillus niger. The number of live bacteria of Lactobacillus plantarum per gram or per milliliter of the composite fermentation agent is 3.5×10 9 ‑6×10 9 CFU, the number of viable Candida utilis is 1×10 9 ‑3×10 9 CFU, the number of viable Aspergillus niger is 1×10 9 ‑3×10 9 CFU. The compound fermentation agent does not use other additives and feed additives, the formula is clean, the compound fermentation agent produces acid quickly during the feed fermentation process, the fermentation cycle is short, and the feed fermentation effect is good.

The invention provides an excrement and urine sewage collection and treatment system and a resource utilization method. The excrement and urine sewage collection and treatment system comprises a closestool, a separation pipeline, a vacuum device and a fermentation device which are sequentially connected, a bottom outlet of the closestool is connected with a defecation pipe and a drainage pipe through a tee joint, the defecation pipe is connected into a vacuum device, and the drainage pipe is connected into a rainwater pipeline. The inner wall of a urinal of the closestool is coated with a non-stick coating, the flushing unit and the lubricating unit are installed on the closestool, the lubricating unit sprays lubricating liquid to the inner wall of the closestool, and excrement and urine can be completely sucked into the vacuum device under the non-flushing condition; the flushing unit flushes the inner wall of the closestool and discharges water into the drainage pipe, flushing water cannot enter a vacuum fermentation system formed by the vacuum device and the fermentation device, and smooth fermentation of dry matter content is guaranteed. According to the excrement and urine sewage collection and treatment system and the resource utilization method, the urban sewage treatment capacity and difficulty can be reduced, continuous and effective fermentation of excrement and urine is guaranteed, and excrement and urine nutrient components are fully and reasonably utilized.

The invention belongs to the technical field of biological medicines, and discloses a method for preparing clavulanic acid by a biological fermentation method. The method comprises the following steps: inoculating a seed bacterial liquid of streptomyces clavuliformis into a fermentation culture medium for fermentation culture, and collecting a fermentation product to obtain clavulanic acid, wherein in the fermentation culture process, an amylase enzymolysis solution is supplemented, the total sugar concentration range of fermentation liquor is maintained to be 6 g / L-12 g / L, and the amylase enzymolysis solution is obtained by performing enzymolysis and liquefaction on starch through alpha-amylase. By adopting the method, the fermentation titer can be improved, and the production cost of the clavulanic acid can be reduced.

The present invention provides a recombinant human type II collagen fragment, the amino acid sequence is shown in SEQ ID NO.2, and the coding gene sequence is shown in SEQ ID NO.1. The present invention also provides an expression vector and Pichia pastoris engineering bacteria comprising the above-mentioned recombinant human type II collagen fragment coding gene. At the same time, the present invention also provides a preparation and application of the above-mentioned recombinant human type II collagen fragment. The recombinant human type II collagen fragment of the present invention not only has a significant effect on promoting the proliferation and migration of human chondrocytes, but also shows excellent effects of promoting cell migration and promoting adhesion in human immortalized epidermal cells and mouse embryonic fibroblasts, and can be widely used in artificial bones, artificial cartilage, artificial corneas, vitreous bodies, wound repair materials, supporting biomaterials, medical cosmetic surgery, cosmetics and other fields.

The invention belongs to the technical field of bioengineering, and particularly relates to a fermentation product for tonifying spleen and promoting digestion as well as a preparation method and application thereof. The spleen-tonifying digestion-promoting fermentation product is prepared from the following fermentation raw materials in parts by weight: 30 parts of citron, 120 parts of codonopsis pilosula, 60 parts of inula nervosa and 90 parts of fresh ginger through enzymolysis and fermentation. From the perspective of relieving functional dyspepsia, the invention provides the fermented product with the effects of tonifying spleen and helping digestion, and the fermented product is rich in function and good in long-term taking effect.

The invention discloses a Pseudomonas protegens QNF1, a microbial agent and an application thereof. The Pseudomonas protegens QNF1 is an endophyte isolated from the root system of leek and is deposited in the General Microbiological Center of the China Microbiological Culture Collection Administration Committee with a deposit number of CGMCC No. 28587. The fermentation broth, cell-free supernatant and fermentation broth volatiles of the strain have significant antagonistic effects on Monilinia fructicola and have good inhibitory effects on peach fruit brown rot caused by Monilinia fructicola, wherein the inhibition rate of the fermentation broth on peach fruit brown rot is as high as 85.58%, and the inhibition rate of the fermentation broth volatiles on peach fruit brown rot is as high as 98.76%. Pseudomonas protegens QNF1 is a potential strain of biological control with good control effect and safe ecology, and its control agent has a simple fermentation process, low production cost and broad market application prospects.

The utility model provides an excrement and urine sewage collection and treatment system. The excrement and urine sewage collection and treatment system comprises a closestool, a separation pipeline, a vacuum device and a fermentation device, a bottom outlet of the closestool is connected with a defecation pipe and a drainage pipe through a tee joint, the defecation pipe is connected into a vacuum device, and the drainage pipe is connected into a rainwater pipeline. The inner wall of a urinal of the closestool is coated with a non-stick coating, the flushing unit and the lubricating unit are installed on the closestool, the lubricating unit sprays lubricating liquid to the inner wall of the closestool, and excrement and urine can be completely sucked into the vacuum device under the non-flushing condition; the flushing unit flushes the inner wall of the closestool and discharges water into the drainage pipe, flushing water cannot enter a vacuum fermentation system formed by the vacuum device and the fermentation device, and smooth fermentation of dry matter content is guaranteed. The utility model provides an excrement and urine sewage collection and treatment system which can reduce urban sewage treatment capacity and difficulty, guarantee continuous and effective fermentation of excrement and urine and enable nutrient components of the excrement and urine to be fully and reasonably utilized.

The present invention relates to a strain of Bacillus glutamicum and its bacterial agent and application in degrading nitrogen-containing compounds, belonging to the technical field of microorganisms. Glutamicibacter arilaitensis )XF1‑2, the strain is deposited in the General Microbiology Center of China Microbial Culture Collection Committee, with a deposit number of CGMCC No.32254, a deposit date of October 17, 2024, and an address of the depository institution: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The research glutamicum Bacillus XF1‑2 provided by the present invention has a strong degradation ability for nitrates, nitrites and ammonium salts in sewage, and an addition amount of 0.05‰ can meet the use requirements of sewage treatment. Compared with the existing research glutamicum Bacillus, the ammonia nitrogen removal rate is more than 3.5 times higher.

The application discloses a recombinant Yarrowia lipolytica engineering bacterium for synthesizing borneol from scratch, a construction method and application thereof. The recombinant Yarrowia lipolytica engineering bacterium is constructed by integrating a multi-gene tandem expression cassette containing an orange-flower base pyrophosphate synthase coding gene, a borneol base pyrophosphate synthase coding gene and a hydrolytic enzyme coding gene into a genome H1 site of Yarrowia lipolytica ATCC 201249 by CRISPR-Cas9 gene editing technology. The application first constructs the recombinant Yarrowia lipolytica engineering bacterium for synthesizing borneol from scratch by using a simple carbon source. The engineering bacterium does not need to add any terpene precursor externally, and only uses simple carbon sources such as glucose and sucrose as substrates. The borneol is synthesized from acetyl coenzyme A, a primary metabolic starting material, by a complete MVA pathway and an artificially constructed borneol-specific synthesis pathway, so that the whole biosynthesis of borneol is realized, and the limitation of the prior art on external precursors is broken through.

The invention belongs to the technical field of biological fermentation, and discloses a method for producing L-alanine through fermentation. The method adopts two-stage fermentation, namely aerobic stage fermentation and anaerobic fermentation after 6 hours, and comprises the following steps: inoculating an escherichia coli seed solution into a fermentation culture medium, controlling the fermentation temperature to be 35-40 DEG C, the tank pressure to be 0.02-0.05 Mpa, the PH to be 6.5-7.0 and the residual sugar to be 0.2-0.5%, controlling the dissolved oxygen to be more than 50% in the aerobic stage in the fermentation process, controlling the dissolved oxygen to be 0 after anaerobic fermentation, replacing soy peptone with bone peptone, and fermenting for 1-2 hours. The fermentation process is optimized, the fermentation efficiency of the L-alanine is improved, and the method is simple in process and suitable for large-scale industrial production.

This invention belongs to the field of biotechnology and discloses a strain of *Pseudomonas oxysporum* and its application in alleviating continuous cropping obstacles in plants. The preservation number of *Pseudomonas oxysporum* is CCTCC NO: M2023054. This invention isolated a strain of *Pseudomonas oxysporum* RH60 from soil in a cucumber-pepper rotation system. This strain can effectively solve the problem of aggravated root-knot nematode disease under continuous cucumber cropping conditions and efficiently degrades the autotoxic substance p-hydroxybenzoic acid produced by continuous cucumber cropping, while also promoting plant growth. The *Pseudomonas oxysporum* provided by this invention has potential application prospects in the prevention and control of root-knot nematode disease and in solving continuous cropping obstacles caused by autotoxic substances.

The invention relates to the technical field of valine, in particular to a preparation method of high-purity valine. The preparation method of the high-purity valine comprises culture, fermentation for acid production, purification and post-treatment, and the specific operation of fermentation for acid production comprises the following steps: inoculating a bacillus seed solution obtained after culture into a fermentation culture medium, and fermenting for a period of time in an environment with the ventilatory capacity of 1-1.2 vvm and the rotating speed of 150-180r / min, so as to obtain the high-purity valine. And then inoculating the culture medium with an escherichia coli seed solution obtained after culture, adjusting the ventilatory capacity to 0.2-0.3 vvm, adjusting the rotating speed to 80-100 r / min, and continuing fermentation to obtain a valine fermentation solution. The purity of the high-purity valine obtained by the preparation method disclosed by the invention is not lower than 99.955%.

The present invention belongs to the technical field of fermented extract preparation, and specifically relates to a mulberry leaf fermented extract with a slow-release sugar effect, and a preparation method and application thereof. The present invention first adds saccharomyces cerevisiae separated from the surface of mulberry leaves while ensuring that the content of 1-deoxynojirimycin in the mulberry leaf extract remains unchanged, and utilizes the method of microbial fermentation to fully release and retain the nutrition and active ingredients of the mulberry leaves, and ensures the dissolution rate of the active ingredients of the mulberry leaves while also ensuring safety; secondly, the use of organic solvents is reduced by fermentation, and environmental pollution is avoided; again, the bitterness of the mulberry leaf extract is reduced or even avoided, and the fragrance of mulberry leaf tea is improved; finally, the microbial varieties in the method are easy to obtain, the fermentation process is simple, the use cost is low, and the microbial fermentation is efficient and repeatable, whether it is laboratory small-scale preparation or factory large-scale production, it has cost advantages and has good practical application value.

The invention discloses a biosynthesis method of alpha-lipoic acid, an engineering strain and a preparation method of the engineering strain. The construction of the engineering strain comprises the following steps: transforming lipocaprylamide enzyme Lpa of enterococcus faecalis, constructing the lipocaprylamide enzyme Lpa to an arabinose promoter or a Cold temperature-sensitive promoter, and then replacing the lipocaprylamide enzyme Lpa to a LacZ gene and an lpxM gene of escherichia coli through RED recombination; or the genes of LipD, lplA, lipA and lpA which are subjected to gene code modification are expressed in the saccharomyces cerevisiae through a pESC vector. The engineering strain is cultured to biologically synthesize alpha-lipoic acid, so that the fermentation cost is further reduced, cyclic synthesis is realized, and the yield is greatly increased.

The present invention relates to a fermentation process for improving the solvent production of Clostridium beijerinckii. The present invention improves the solvent production of Clostridium beijerinckii by controlling the production of byproduct glycoproteins. The specific fermentation process is that in the process of fermenting and producing solvents with Clostridium beijerinckii as a fermentation bacterium, a feed containing a nitrogen source is added to the fermentation liquid during the logarithmic growth phase of Clostridium beijerinckii, and the nitrogen source is ammonium acetate, so that the final concentration of ammonium acetate in the fermentation liquid at the end of the addition is 3.3-12.2 g / L; the fermentation produces solvents and suppresses the large-scale production of byproduct glycoproteins. The present invention adds an ammonium acetate solution during the logarithmic growth phase of Clostridium beijerinckii fermentation without controlling the pH of the fermentation liquid, so that Clostridium beijerinckii can supplement the nitrogen source during the fermentation process without causing excessive nitrogen source, can improve the utilization rate of substrate glucose, can improve the total solvent production, and can also suppress the large-scale production of byproduct glycoproteins.

The invention discloses a method for preparing ethanol through detoxification-free fermentation of furfural residues and application. The method comprises the following steps: S1, carrying out high-solid-content enzymolysis on acidic furfural residues which are not subjected to any detoxification pretreatment to obtain furfural residue enzymatic hydrolysate; s2, liquefying and saccharifying the waste starch to obtain a starch saccharification liquid; and S3, mixing the furfural residue enzymatic hydrolysate and the starch saccharification liquid in proportion, inoculating zymomonas mobilis, and carrying out co-fermentation to produce ethanol. According to the method, key technologies such as detoxification-free treatment, high-solid-content enzymolysis and co-fermentation with starch saccharification liquid are combined, and the steps of neutralization, water washing and deep detoxification which are necessary in a traditional process are thoroughly abandoned, so that the process is greatly simplified, water resources are saved, the treatment cost is reduced, and the problem of toxicity of inhibitors in raw materials is effectively solved. The method provides an efficient, economical and environment-friendly brand-new way for realizing high-valued resource utilization of the furfural residues and the waste starch.

Disclosed are a hybrid dialkylphosphinate salt, a method for preparing same, and use thereof. The hybrid dialkylphosphinate salt is selected from at least one of the compounds represented by Formula (I). The hybrid dialkylphosphinate salt of Formula (I) provided herein features a low required loading level, high flame retardant efficiency for various polymers, and good thermal stability. The present invention overcomes the disadvantage of low flame retardant efficiency of diethylphosphinate in polymers as well as low thermal stability and large dust of dipropylphosphinate. The hybrid dialkylphosphinate salt of Formula (I) can be widely applied to flame retardant polymers which require high-temperature processing.

The invention discloses a chemostat culture device and method for controlling the steady state of growth and metabolism of food microorganisms, the device comprises a reactor and storage containers, the storage containers are divided into a first storage container, a second storage container and a third storage container, the first storage container is used for storing a fresh culture medium, and the second storage container and the third storage container are used for storing an old culture medium; the first storage container is connected with the reactor through a pipeline, and a first peristaltic pump is arranged on the pipeline so as to pump a fresh culture medium stored in the first storage container into the reactor; the reactor is connected with the second storage container and the third storage container through a pipeline and a three-way valve; a second peristaltic pump is arranged on the pipeline, so that the old culture medium fermented in the reactor is pumped into the second storage container and / or the third storage container. The device is provided with a plurality of storages and is matched with the peristaltic pump, so that the constant requirements of various growth rates of different food microorganisms in the logarithmic growth phase are met, and the growth and metabolism steady state of the food microorganisms is controlled.

The invention belongs to the field of microorganisms and fermentation engineering, and discloses recombinant escherichia coli for producing butyrolactam as well as a construction method and application of the recombinant escherichia coli. According to the invention, a dynamic molecular switch for regulating and controlling a central carbon metabolism pathway is constructed through a molecular biological technology, so that the recombinant escherichia coli realizes direct synthesis from glucose to butyrolactam. Compared with the prior art, the invention not only provides a method for de novo synthesis of butyrolactam, but also avoids the problems of complex production industry, expensive precursor substance or cofactor addition and the like caused by chemical synthesis and in-vitro multi-enzyme catalysis. Therefore, the production process is simple, safe and green, meanwhile, the yield of butyrolactam also reaches a relatively high level, and the method is a method with an industrial preparation prospect.

According to the algistat, the whole overground part of Artemisia annua is dried at 60 DEG C and crushed to 150 meshes, tea seed cake powder is crushed to 150-mesh and 80-mesh trichoderma harzianum leavening is used as raw materials, and the algistat is obtained by uniformly mixing the raw materials and the trichoderma harzianum leavening and the trichoderma harzianum leavening and the trichoderma harzianum leavening and the trichoderma harzianum leavening and the trichoderma harzianum leavening and the trichoderma harzianum leavening and the trichoderma harzianum leavening. The preparation method of the algicide for the premnant algae in the stichopus japonicus circle is simple, the raw materials are wide in source, low in cost and high in algicide inhibition efficiency, outbreak of the premnant algae can be controlled, harm of toxin released by the premnant algae to stichopus japonicus is reduced, resistance is not prone to being generated, and the algicide has no toxic or side effect on the stichopus japonicus and no harm to the environment and is worthy of popularization and application. The application effect is obvious, and the market prospect is wide.