750results about How to "The fermentation process is simple" patented technology

The present invention provides a method for converting lignocellulosic material into biofuel. In particular embodiments, the method comprises pre-treating lignocellulosic material by dissolving the material in ionic liquids. The pretreated lignocellulosic material can be isolated, such as by precipitation with a regenerating solvent (e.g., water), and be used directly in the formation of biofuel, including undergoing hydrolysis to form sugar and fermentation to form fuel, such as bioethanol. The ionic liquid can be recycled for further use, such as by evaporation of the water introduced during precipitation, and the recycling provides a route to a hemicellulose rich fraction and an ionic liquid of consistent quality and wood dissolution characteristics. The recovered hemicelluloses are of significant utilization potential toward commodity and specialty applications.

The invention discloses a streptomyces corchorusii strain NF0919, which is collected in China General Microbiological Culture Collection Center, and has the collection number CGMCC No. 3968. The strain can be used for preventing and treating rhizoctonia cerealis, rice false smut, rice sheath blight disease and other various plant diseases.

The invention discloses a bacillus licheniformis strain which is preserved as bacillus licheniformis WX-02 with the preserving number of NO: M208065 and the preserving date of April 24th, 2008 and the preserving unit of CCTCC. The invention also discloses an application of the bacillus licheniformis strain and a method for producing poly-gamma-glutamic acid by using the bacillus licheniformis strain. Poly-gamma-glutamic acid products are produced by processes of seed solution preparation and liquid submerged fermentation, and the strain has stable genetic performance of the strain, high production efficiency and low production cost. When the bacillus licheniformis strain of the invention and the fermentation method thereof are used for preparing 3000L of poly-gamma-glutamic acid, the yield of a fermentation tank can reach 35.05g/l.

The invention provides a probiotic fruit and vegetable enzyme drink and a preparation method thereof. Fruits and vegetables as well as four-in-one PPHR Lactobacillus powder (including Lactobacillus paracasei, L. plantarum, L. helveticus and L. rhamnosus) are mixed and the obtained mixture is fermented, and the obtained fermented product is the probiotic fruit and vegetable enzyme drink. Compared with the prior art, the preparation method has the advantages that the fermentation process can be shortened, the fermentation process can be simplified, the nutritional value of fruit and vegetable juice can be increased, the fruit and vegetable juice is endowed with higher oxidation resistance, and the application range of Lactobacillus in food, particularly drinks, is broadened.

The invention relates to an aroniamelanocarpa fruit wine and a brewing method thereof. The fruit of aroniamelanocarpa is taken as raw material and is made into fruit juice, fruit wine microzyme is selected for use and put into the fruit juice according to the proportion being 5-10% of the weight of the fruit juice, and the aroniamelanocarpa fruit wine is brewed with the alcohol content of 5-13% (v/v) and the total sugar content of 4.0-80.0g/L. The brewing technique of the invention comprises preparing the fruit juice, inoculation and fermentation, aging and store maturity, purifying wine body as well as filtration and sterilization, and the most advanced fermenting and purifying technique can be adopted by the production. The product of the invention has unique flavor, slightly acerbic and fresh taste, mellow mouthfeel, pleasing aroma and wide applicable people. The fruit wine contains multiple nutritional functional components such as anthocyanin, flavone, polyphenol active substance, vitamins antioxidant, mineral composition, polyoses, organic acid and the like which are rich in the fruit of aroniamelanocarpa, and has good effects of oxidation resistance, ageing prevention, blood pressure regulation, vessel relaxing, blood-fat reduction, anti-inflammation and detoxification, radiation resistance, etc.

The invention discloses a preparation method of original sugarcane vinegar. The method includes pressing sugarcanes, extracting sugarcane mix, boiling for sterilization, diluting with aseptic purified water to prepare fermentation liquid, inoculating the fermentation liquid to activated fruit wine yeast, and allowing for alcoholic fermentation for 14-20 days at the fermentation temperature of 25-32 DEG C; when initial alcoholic strength reaches (5+/-0.5) DEG C, adding activated LB acetic bacteria at 20-28 DEG C for acetic fermentation for 13-28 days; filtering and sterilizing after acetic fermentation to obtain original sugarcane vinegar. The preparation method is simple in fermentation process which is convenient to master.

The invention covers a novel treatment strategy that considerably improves conventional probiotic treatments of inflammatory bowel diseases, irritable bowel syndrome and other gastrointestinal disorders. Both probiotic microorganisms and the carrier of the probiotic microorganisms in form of a fermented cereal gruel are used as treatment effectors. Phospholipids may also be an effecter. The novel treatment strategy is capable of removing the symptoms of inflammatory bowel diseases regardless of a mild, moderate or severe stage of the disease.

A liquid fermentation process for the Chinese pilous spore includes such steps as getting bacterial spawn, Erlenmeyer flask culture of bacterial spawns, liquid seed culture, and liquid fermenting. Its advantags are high successful rate, low cost and no environmental pollution. The resultant mycelia can be used for medical or health-care product. The fermented liquid can be used as oral liquir or beverage.

The invention belongs to the technical field of production of vitamin A and its derivatives, in particular to a method for preparing vitamin A acetate. The method comprises the following steps: (1) mixing triphenylphosphine and acid in supercritical CO2, and then contacting the obtained mixture with vinyl-beta-ionol for salt formation to obtain C15 phosphine salt; (2) after adding solvent water, ionic liquid and C5 aldehyde to the finished system of step (1) , CO2 is released for solvent replacement, and the materials are uniformly mixed; (3) adding an extractant to the system after the solvent replacement of step (2), stirring and adding an alkali solution for Wittig reaction to obtain the vitamin A acetate. The method of the invention has mild reaction conditions, safe production and easy control, and the purity and reaction yield of the final product are high.

The invention provides a genetic engineering bacterium capable of producing uridine at high yield as well as a building method and application thereof. The genetic engineering bacterium is characterized in that pyrimidine nucleoside operons pyrBCAKDFE with the nucleotide sequence shown as SEQ ID NO:1 is integrated on a genome of colon bacillus; the starting is realized by a strong promoter P[trc];a uridine synthesis path is reconstituted; the self PRPP synthetase coding gene prsA on the genome is subjected to dual copying, and the starting is realized by the strong promoter P[trc]; meanwhile,the activity of udk, udp, and rihA, rihB and rihC is lacked; the thrA activity is lacked; the argF activity is lacked. The genetic engineering bacterium is applied to fermentation production of uridine; 40 to 67g/L of uridine can be produced after the fermentation is performed for 40 to 70h in a 5L fermentation tank; the maximum production intensity can reach 1.5g/(L*h); the glucoside conversionrate is 15 to 25 percent; the genetic engineering bacterium belongs to the highest level for producing the uridine by the fermentation method reported in the prior art.

The invention discloses a method for producing alcohol by co-fermenting glucose with recombinant Saccharomyces cerevisiae strains for industrial production. The invention comprises the steps of: (1) selecting recombinant Saccharomyces cerevisiae NAN127 (CGMCC No.1848), (2) slant-culturing, (3) culturing for seed, (4) enlargement-culturing, (5) fermenting, and (6) distilling to obtain alcohol. The method can utilize glucose and xylose in the medium at the same time, has the advantages of high utilization of xylose, high alcohol converting rate, and broad prospect in production alcohol with lignocellulose.

The invention discloses a purpose of lignin degrading bacterium, and particularly relates to a method for synthesizing a biological plastic precursor-polyhydroxyalkanoate by utilizing lignin degrading bacterium (Cupriavidus basilensis B-8) with a collection number of CGMCC (China General Microbiological Culture Collection Center) No. 4240. A bacterial strain can grow by utilizing lignin (the lignin concentration of a growth medium is 1g/L to 6g/L) which is not subjected to pretreatment as a unique carbon source, and is used for synthesizing a homopolymer of biological plastic-polyhydroxyalkanoate in the condition (the concentration of nitrogen is less than 60mg/L) that a nitrogen source is short. By using a culture and a production method which are provided by the invention, the carbon source and the pretreatment cost of the carbon source are greatly decreased; the method has large-scale production and industrialization potential.

The invention relates to a production process for bioorganic fertilizer with high bacteria content. The production process comprises the following steps: separately activating a trichoderma viride strain and a trichoderma pseudokoningii strain; separately activating a bacillus amyloliquefaciens strain, a bacillus subtilis strain and a candida utilis strain; carrying out step-by-step enlarged culture and the like. A preparation method provided by the invention has the advantages of low cost, simple process, high operability and convenience in popularization and application; the obtained bioorganic fertilizer is high in content of effective living bacteria; a large amount of corn stalk resources can be really and efficiently converted, soil is remediated, the fertility of the soil is improved, and wastes are changed into valuables; in addition, the problem of environmental pollution caused by straw burning can be relived to a certain degree.

The invention discloses a method for producing 2,3-butanediol by using starch raw materials, which comprises the following steps: subjecting the starch raw materials to treatment by alpha-amylase and maltogenic amylase; and producing the 2,3-butanediol by carrying out separate hydrolysis and fermentation or simultaneous sacchrification and fermentation under an aseptic condition, using saccharification liquid or liquefaction liquid as a fermentable substrate and using Klesiella pneumoniae as a fermentable strain. Finally the concentration of the 2,3-butanediol reaches 43 to 112g/L, and the final concentration of acetoin and the final concentration of the 2,3-butanediol reach 45 to 121g/L. The method has the advantages of using the starch raw materials to produce a high-value platform compound by fermentation, reducing the raw material cost of the production of the 2,3-butanediol by the fermentation and establishing the optimal fermentation condition for the production of the 2,3-butanediol by the sacchrification and fermentation of the starch raw materials. The production of the 2,3-butanediol by the simultaneous sacchrification and fermentation of the invention can save energy and equipment investment, reduce fermentation period and improve production efficiency.

This invention is to the removal of organics and nutrients from wastewater. More specifically, the invention addresses the removal of nutrients such as nitrogen and/or phosphorus from wastewater and has specific adaptations that can be of added benefit in small systems including septic tanks, cluster wastewater systems, and other small treatment plants. The invention can also be used for larger treatment works and for sidestream treatment systems. The invention also has adaptations that can be used to treat wastewater in low gravity and other difficult environments. The invention also has adaptations to permit rapid startup, to induce dormancy, and to preserve organisms for extended periods of time.

The present invention relates to use of gene engineering in modifying antibiotic ingredient, more specificly it refer to blocking and destroying 3-O-acyltransferase which take part in the production of component II and III of bitespiramycin-producing bacteria. The engineered bacteria is obtained by homologous gene double exchange and employs 4'' -isovalerylspiramycin I as main ingredient. The engineered bacteria can simplify the production process, lower the production cost and control the quality standard.

The invention relates to a Trichoderma koningiopsis TZ-11 bacterial strain, an application and a method for preparing a preparation of the Trichoderma koningiopsis TZ-11 bacterial strain. According to the bacterial strain, the wettable powder prepared by conducting solid fermentation can be used for preventing and treating the anthracnose, caused by infections of Glomerella cingulata, of many crops such as pepper, mango, banana and loquat and especially for preventing and treating the grape anthracnose, the strawberry anthracnose and other plant diseases caused by infection of Glomerella cingulata. The field test result shows that the fungicide has a good preventing and controlling effect on the grape anthracnose and the strawberry anthracnose, the bacterial strain fermenting technology is simple, and the production cost is low. The fungicide living spore content is high, and the storage quality is stable.

The invention discloses a microbial fermentation agent for cottonseed meal detoxification and a preparation method thereof. According to the preparation method, saccharomyces cerevisiae and bacillus subtilis serve as active strains and are subjected to expanding culture by using liquid fermentation, a carrier is added, and then, the microbial fermentation agent is prepared. According to the microbial fermentation agent, free gossypol in cottonseed meal can be degraded effectively, crude fibers are degraded and softened, the palatability of the cottonseed meal is improved, a large number of nutrition and hormone substances, such as active probiotics, bioactive small-peptide amino acids, various biochemical enzymes, somatomedins and the like, are secreted and synthesized, and the immunity of animals is effectively improved by microbial thalli protein, beta-glucan and the like in the microbial fermentation agent. The microbial fermentation agent further has the advantages that the strains and fermentation processes are simple, the cost is low, the consumption of the strains in cottonseed meal fermentation is low, the cost for the cottonseed meal fermentation is low, and the like.

The invention discloses a lipopeptide-producing bacillus pumilus, and classification thereof is named to be Bacillus pumilus Y8A which is preserved by China Center for Type Culture Collection (CCTCC) in Wuhan University with the preservation number of CCTCCM 209108. The invention also discloses an application of the lipopeptide-producing bacillus pumilus in promoting the micro-biological degradation process of petroleum and/or cyhalothrin pesticide. Bacterial strain Y8A of the invention can produce lipopeptide biosurfactant with high efficiency and stability, thereby effectively promoting the micro-biological degradation process of petroleum and/or cyhalothrin pesticide degrading bacteria. Bacterial strain Y8A with the function of efficiently producing lipopeptide is expected to work as a new-generation bacteria and cosolvent for antibiotic production and, is widely used in agriculture, medicine, cosmetic and food industries, and can be used for restoration of pollutant environment together with other organic pollution degrading microbes. The bacterial strain is resistant to a plurality of antibiotics with easy fermentation course and convenient operation.