755results about How to "The fermentation process is simple" patented technology

The present invention provides a method for converting lignocellulosic material into biofuel. In particular embodiments, the method comprises pre-treating lignocellulosic material by dissolving the material in ionic liquids. The pretreated lignocellulosic material can be isolated, such as by precipitation with a regenerating solvent (e.g., water), and be used directly in the formation of biofuel, including undergoing hydrolysis to form sugar and fermentation to form fuel, such as bioethanol. The ionic liquid can be recycled for further use, such as by evaporation of the water introduced during precipitation, and the recycling provides a route to a hemicellulose rich fraction and an ionic liquid of consistent quality and wood dissolution characteristics. The recovered hemicelluloses are of significant utilization potential toward commodity and specialty applications.

The invention discloses a streptomyces corchorusii strain NF0919, which is collected in China General Microbiological Culture Collection Center, and has the collection number CGMCC No. 3968. The strain can be used for preventing and treating rhizoctonia cerealis, rice false smut, rice sheath blight disease and other various plant diseases.

A process for obtaining a product sugar stream from cellulosic biomass is disclosed. In one process, the cellulosic biomass is pretreated at a pH between about 0.4 to 2.0 by adding one or more than one acid to produce a pretreated cellulosic biomass comprising acetic acid. One or more than one base is then added to the pretreated cellulosic biomass to adjust the pretreated cellulosic biomass to a pH of about 4.0 to about 6.0 to produce a neutralized cellulosic biomass comprising inorganic salt and acetate salt. The neutralized biomass is then hydrolyzed by cellulase enzymes to produce a crude sugar stream. Insoluble residue is separated from the crude sugar stream and the resulting clarified sugar stream is treated using ion exclusion chromatography at about pH 5.0 to about 10.0 to produce one or more raffinate streams and a product stream. The raffinate stream comprises inorganic salts and acetate salts, and the product stream comprises sugar. The product stream may then be fermented or otherwise further processed. In an alternate process, a product sugar stream is obtained from a crude sugar stream that is produced from conversion of cellulosic biomass to sugar. The cellulosic biomass may be produced using any suitable method. In this process the crude sugar stream is treated using ion exclusion chromatography at about pH 5.0 to about 10.0 to produce one or more than one raffinate stream comprising sulfate and acetate salts, and a product stream comprising sugar, and the product sugar stream is obtained.

The invention discloses a bacillus licheniformis strain which is preserved as bacillus licheniformis WX-02 with the preserving number of NO: M208065 and the preserving date of April 24th, 2008 and the preserving unit of CCTCC. The invention also discloses an application of the bacillus licheniformis strain and a method for producing poly-gamma-glutamic acid by using the bacillus licheniformis strain. Poly-gamma-glutamic acid products are produced by processes of seed solution preparation and liquid submerged fermentation, and the strain has stable genetic performance of the strain, high production efficiency and low production cost. When the bacillus licheniformis strain of the invention and the fermentation method thereof are used for preparing 3000L of poly-gamma-glutamic acid, the yield of a fermentation tank can reach 35.05g / l.

The invention provides a probiotic fruit and vegetable enzyme drink and a preparation method thereof. Fruits and vegetables as well as four-in-one PPHR Lactobacillus powder (including Lactobacillus paracasei, L. plantarum, L. helveticus and L. rhamnosus) are mixed and the obtained mixture is fermented, and the obtained fermented product is the probiotic fruit and vegetable enzyme drink. Compared with the prior art, the preparation method has the advantages that the fermentation process can be shortened, the fermentation process can be simplified, the nutritional value of fruit and vegetable juice can be increased, the fruit and vegetable juice is endowed with higher oxidation resistance, and the application range of Lactobacillus in food, particularly drinks, is broadened.

The invention relates to an aroniamelanocarpa fruit wine and a brewing method thereof. The fruit of aroniamelanocarpa is taken as raw material and is made into fruit juice, fruit wine microzyme is selected for use and put into the fruit juice according to the proportion being 5-10% of the weight of the fruit juice, and the aroniamelanocarpa fruit wine is brewed with the alcohol content of 5-13% (v / v) and the total sugar content of 4.0-80.0g / L. The brewing technique of the invention comprises preparing the fruit juice, inoculation and fermentation, aging and store maturity, purifying wine body as well as filtration and sterilization, and the most advanced fermenting and purifying technique can be adopted by the production. The product of the invention has unique flavor, slightly acerbic and fresh taste, mellow mouthfeel, pleasing aroma and wide applicable people. The fruit wine contains multiple nutritional functional components such as anthocyanin, flavone, polyphenol active substance, vitamins antioxidant, mineral composition, polyoses, organic acid and the like which are rich in the fruit of aroniamelanocarpa, and has good effects of oxidation resistance, ageing prevention, blood pressure regulation, vessel relaxing, blood-fat reduction, anti-inflammation and detoxification, radiation resistance, etc.

The invention relates to a process for obtaining a product sugar stream from a cellulosic biomass, comprising pretreating the cellulosic biomass to hydrolyze a portion of the cellulose and hemicellulose to produce glucose, acetic acid and a sugar monomer, adding base to the pretreated cellulosic biomass to produce inorganic salt and acetate salt; hydrolyzing the neutralized cellulosic biomass with cellulase enzymes and separating insoluble residue from the resulting sugar stream; treating the clarified sugar stream using exclusion chromatography at a pH of 5 to 10 to produce a product sugar stream.

The invention relates to a self-propelled turning device for sludge compost and a treatment method and belongs to environmentally-friendly solid waste treatment. The method comprises that: a mixing step, an aerobic compost period, a thoroughly decomposing step, a processing and compost production step and an odor treatment step, wherein the mixing step aims to adjust the moisture and the carbon nitrogen rate of dewatered sludge, improve the loosening degree, increase a contact area with air and contributes to aerobic fermentation; in the aerobic compost period, the oxygen is supplied through forced ventilation, and organic matters in materials begin to ferment under the action of aerobes; the thoroughly composting step comprises that: the sludge subjected to compost fermentation is not thoroughly decomposed, and is required to continuously undergo a thoroughly decomposing and ageing stage, namely secondary fermentation; the processing and compost production step comprises that: the sludge compost is processed according to fertilizer use and market demand to serve as a product for sales so as to improve overall economic efficiency; and the odor treatment step comprises that: odor is generated in the process of aerobic compost and can be discharged after deodorization treatment. In recent years, solid wastes have become important environmental problems limiting urban development. The composting treatment is a way to utilize solid waste resources and is gradually taken into account at home and abroad.

The invention discloses a preparation method of original sugarcane vinegar. The method includes pressing sugarcanes, extracting sugarcane mix, boiling for sterilization, diluting with aseptic purified water to prepare fermentation liquid, inoculating the fermentation liquid to activated fruit wine yeast, and allowing for alcoholic fermentation for 14-20 days at the fermentation temperature of 25-32 DEG C; when initial alcoholic strength reaches (5+ / -0.5) DEG C, adding activated LB acetic bacteria at 20-28 DEG C for acetic fermentation for 13-28 days; filtering and sterilizing after acetic fermentation to obtain original sugarcane vinegar. The preparation method is simple in fermentation process which is convenient to master.

The present invention provides a method for converting lignocellulosic material into biofuel. In particular embodiments, the method comprises pre-treating lignocellulosic material by dissolving the material in ionic liquids. The pretreated lignocellulosic material can be isolated, such as by precipitation with a regenerating solvent (e.g., water), and be used directly in the formation of biofuel, including undergoing hydrolysis to form sugar and fermentation to form fuel, such as bioethanol. The ionic liquid can be recycled for further use, such as by evaporation of the water introduced during precipitation, and the recycling provides a route to a hemicellulose rich fraction and an ionic liquid of consistent quality and wood dissolution characteristics. The recovered hemicelluloses are of significant utilization potential toward commodity and specialty applications.

The invention discloses a processing method of flower fragrance type kung fu black tea. The method comprises the steps of fresh leaf picking, sun drying, fine manipulation of green tea leaves, quick-freezing withering, unfreezing, rolling, fermentation, first firing, full firing, fragrance increasing, vacuum packaging and the like. The technology is improved for solving the problems that when Lingyun white tea variety black tea is processed, taste is light in freshness and sweetness, fragrance is light and style and variety are monotonous. The processing method comprises the specific steps that tea shoots with two leaves and a bud or with three leaves and a bud of Lingyun white tea trees are picked as raw materials, and technological parameters of sunlight withering, fine manipulation of green tea leaves, freezing withering, rolling, fermentation, full-fire drying, drying and fragrance increasing are enhanced so as to improve a traditional processing method, so that the product has the quality style characteristics that strips are compact and uniform, color and luster are black and smooth, the soup color is orange red and bright, the fragrance is rich and lasting, the taste is mellow and refreshing and leave bottoms are red, bright and uniform, and the black tea has obvious nectar fragrance besides sweet fragrance, and meets requirements of different consumers.

The invention covers a novel treatment strategy that considerably improves conventional probiotic treatments of inflammatory bowel diseases, irritable bowel syndrome and other gastrointestinal disorders. Both probiotic microorganisms and the carrier of the probiotic microorganisms in form of a fermented cereal gruel are used as treatment effectors. Phospholipids may also be an effecter. The novel treatment strategy is capable of removing the symptoms of inflammatory bowel diseases regardless of a mild, moderate or severe stage of the disease.

A liquid fermentation process for the Chinese pilous spore includes such steps as getting bacterial spawn, Erlenmeyer flask culture of bacterial spawns, liquid seed culture, and liquid fermenting. Its advantags are high successful rate, low cost and no environmental pollution. The resultant mycelia can be used for medical or health-care product. The fermented liquid can be used as oral liquir or beverage.

The invention belongs to the technical field of production of vitamin A and its derivatives, in particular to a method for preparing vitamin A acetate. The method comprises the following steps: (1) mixing triphenylphosphine and acid in supercritical CO2, and then contacting the obtained mixture with vinyl-beta-ionol for salt formation to obtain C15 phosphine salt; (2) after adding solvent water, ionic liquid and C5 aldehyde to the finished system of step (1) , CO2 is released for solvent replacement, and the materials are uniformly mixed; (3) adding an extractant to the system after the solvent replacement of step (2), stirring and adding an alkali solution for Wittig reaction to obtain the vitamin A acetate. The method of the invention has mild reaction conditions, safe production and easy control, and the purity and reaction yield of the final product are high.

The invention discloses a phellinus igniarius mycelium liquid fermentation method by utilizing a fungal elicitor for improving the yield of flavone of phellinus igniarius, which comprises the steps of: inoculating a fungal elicitor strain for activated culture, and then inoculating and culturing a fungal block to obtain a fungal elicitor mycelium through filtration; then drying, grinding and crushing the fungal elicitor mycelium, adding the crushed fungal elicitor mycelium into ethanol for soaking, filtering the obtained solution, collecting filter residue, orderly adding a mixed solution of chloroform and normal butanol, acetone and water into the filter residue for washing, adding trifluoroacetic acid into a dry substance after the air-drying, performing boiling water bath for 0.5 to 2 hours, then filtering the obtained solution, taking a filtrate for neutralization, standing the filtrate, using a microporous filtering film with the pore diameter between 0.4 and 5mu m to filter the filtrate for sterilization to obtain the fungal elicitor, and cryopreserving the fungal elicitor at the temperature of between 10 and 20 DEG C below zero; inoculating a phellinus igniarius strain for activated culture, and then inoculating a fungal block for fermentation culture; and adding the fungal elicitor into the phellinus igniarius to continue culturing to obtain a phellinus igniarius mycelium within 1 to 5 days of liquid fermentation culture of the phellinus igniarius. The method has simple process, low cost and remarkable effect, can greatly improve the content of the flavone of phellinus igniarius cells, and is a production method with industrialization prospect.

The invention provides a genetic engineering bacterium capable of producing uridine at high yield as well as a building method and application thereof. The genetic engineering bacterium is characterized in that pyrimidine nucleoside operons pyrBCAKDFE with the nucleotide sequence shown as SEQ ID NO:1 is integrated on a genome of colon bacillus; the starting is realized by a strong promoter P[trc];a uridine synthesis path is reconstituted; the self PRPP synthetase coding gene prsA on the genome is subjected to dual copying, and the starting is realized by the strong promoter P[trc]; meanwhile,the activity of udk, udp, and rihA, rihB and rihC is lacked; the thrA activity is lacked; the argF activity is lacked. The genetic engineering bacterium is applied to fermentation production of uridine; 40 to 67g / L of uridine can be produced after the fermentation is performed for 40 to 70h in a 5L fermentation tank; the maximum production intensity can reach 1.5g / (L*h); the glucoside conversionrate is 15 to 25 percent; the genetic engineering bacterium belongs to the highest level for producing the uridine by the fermentation method reported in the prior art.

A solid-liquid filtering method and system which comprises a thick band (7) of assembled filaments prepared by closely arranging paralleled monofilaments of predetermined length, a means for fixing (11) one end portion of said band and a network support (4) for keeping said band in a plane situation at proper location when said band is forced to turn in a regular direction, one end portion of said monofilaments being fixed and the other end portion being floated.

The invention belongs to the field of fermentation engineering and discloses corynebacterium glutamicum and a method for preparing L-ornithine and salts thereof by using the same. The corynebacterium glutamicum is corynebacterium glutamicum 1006 with the collection number of CGMCCNo.3663. The method comprises the following steps of: culturing the corynebacterium glutamicum in a culture medium containing carbon sources, nitrogen sources and inorganic salts at the temperature of between 28 and 30 DEG C to generate L-ornithine fermentation liquor; removing the thalli of the fermentation liquor by using ceramic membranes of 1 to 15 ten thousand Dalton; separating the fermentation liquor by using cation exchange resins to obtain L-ornithine solution; and decoloring and crystallizing the L-ornithine solution by a hydrochloric acid and ethanol method to obtain L-ornithine hydrochloride. The method has the advantages of simple thallus cultivation and contribution to industrialized mass production. The accumulation of the L-ornithine prepared from the corynebacterium glutamicum reaches 35 to 45g / L.

The invention discloses a method for producing alcohol by co-fermenting glucose with recombinant Saccharomyces cerevisiae strains for industrial production. The invention comprises the steps of: (1) selecting recombinant Saccharomyces cerevisiae NAN127 (CGMCC No.1848), (2) slant-culturing, (3) culturing for seed, (4) enlargement-culturing, (5) fermenting, and (6) distilling to obtain alcohol. The method can utilize glucose and xylose in the medium at the same time, has the advantages of high utilization of xylose, high alcohol converting rate, and broad prospect in production alcohol with lignocellulose.

The invention discloses a purpose of lignin degrading bacterium, and particularly relates to a method for synthesizing a biological plastic precursor-polyhydroxyalkanoate by utilizing lignin degrading bacterium (Cupriavidus basilensis B-8) with a collection number of CGMCC (China General Microbiological Culture Collection Center) No. 4240. A bacterial strain can grow by utilizing lignin (the lignin concentration of a growth medium is 1g / L to 6g / L) which is not subjected to pretreatment as a unique carbon source, and is used for synthesizing a homopolymer of biological plastic-polyhydroxyalkanoate in the condition (the concentration of nitrogen is less than 60mg / L) that a nitrogen source is short. By using a culture and a production method which are provided by the invention, the carbon source and the pretreatment cost of the carbon source are greatly decreased; the method has large-scale production and industrialization potential.

The invention relates to a production process for bioorganic fertilizer with high bacteria content. The production process comprises the following steps: separately activating a trichoderma viride strain and a trichoderma pseudokoningii strain; separately activating a bacillus amyloliquefaciens strain, a bacillus subtilis strain and a candida utilis strain; carrying out step-by-step enlarged culture and the like. A preparation method provided by the invention has the advantages of low cost, simple process, high operability and convenience in popularization and application; the obtained bioorganic fertilizer is high in content of effective living bacteria; a large amount of corn stalk resources can be really and efficiently converted, soil is remediated, the fertility of the soil is improved, and wastes are changed into valuables; in addition, the problem of environmental pollution caused by straw burning can be relived to a certain degree.

The invention discloses a method for producing 2,3-butanediol by using starch raw materials, which comprises the following steps: subjecting the starch raw materials to treatment by alpha-amylase and maltogenic amylase; and producing the 2,3-butanediol by carrying out separate hydrolysis and fermentation or simultaneous sacchrification and fermentation under an aseptic condition, using saccharification liquid or liquefaction liquid as a fermentable substrate and using Klesiella pneumoniae as a fermentable strain. Finally the concentration of the 2,3-butanediol reaches 43 to 112g / L, and the final concentration of acetoin and the final concentration of the 2,3-butanediol reach 45 to 121g / L. The method has the advantages of using the starch raw materials to produce a high-value platform compound by fermentation, reducing the raw material cost of the production of the 2,3-butanediol by the fermentation and establishing the optimal fermentation condition for the production of the 2,3-butanediol by the sacchrification and fermentation of the starch raw materials. The production of the 2,3-butanediol by the simultaneous sacchrification and fermentation of the invention can save energy and equipment investment, reduce fermentation period and improve production efficiency.

This invention is to the removal of organics and nutrients from wastewater. More specifically, the invention addresses the removal of nutrients such as nitrogen and / or phosphorus from wastewater and has specific adaptations that can be of added benefit in small systems including septic tanks, cluster wastewater systems, and other small treatment plants. The invention can also be used for larger treatment works and for sidestream treatment systems. The invention also has adaptations that can be used to treat wastewater in low gravity and other difficult environments. The invention also has adaptations to permit rapid startup, to induce dormancy, and to preserve organisms for extended periods of time.

The present invention relates to use of gene engineering in modifying antibiotic ingredient, more specificly it refer to blocking and destroying 3-O-acyltransferase which take part in the production of component II and III of bitespiramycin-producing bacteria. The engineered bacteria is obtained by homologous gene double exchange and employs 4'' -isovalerylspiramycin I as main ingredient. The engineered bacteria can simplify the production process, lower the production cost and control the quality standard.

The invention relates to a Trichoderma koningiopsis TZ-11 bacterial strain, an application and a method for preparing a preparation of the Trichoderma koningiopsis TZ-11 bacterial strain. According to the bacterial strain, the wettable powder prepared by conducting solid fermentation can be used for preventing and treating the anthracnose, caused by infections of Glomerella cingulata, of many crops such as pepper, mango, banana and loquat and especially for preventing and treating the grape anthracnose, the strawberry anthracnose and other plant diseases caused by infection of Glomerella cingulata. The field test result shows that the fungicide has a good preventing and controlling effect on the grape anthracnose and the strawberry anthracnose, the bacterial strain fermenting technology is simple, and the production cost is low. The fungicide living spore content is high, and the storage quality is stable.

The invention discloses a microbial fermentation agent for cottonseed meal detoxification and a preparation method thereof. According to the preparation method, saccharomyces cerevisiae and bacillus subtilis serve as active strains and are subjected to expanding culture by using liquid fermentation, a carrier is added, and then, the microbial fermentation agent is prepared. According to the microbial fermentation agent, free gossypol in cottonseed meal can be degraded effectively, crude fibers are degraded and softened, the palatability of the cottonseed meal is improved, a large number of nutrition and hormone substances, such as active probiotics, bioactive small-peptide amino acids, various biochemical enzymes, somatomedins and the like, are secreted and synthesized, and the immunity of animals is effectively improved by microbial thalli protein, beta-glucan and the like in the microbial fermentation agent. The microbial fermentation agent further has the advantages that the strains and fermentation processes are simple, the cost is low, the consumption of the strains in cottonseed meal fermentation is low, the cost for the cottonseed meal fermentation is low, and the like.

The invention discloses a lipopeptide-producing bacillus pumilus, and classification thereof is named to be Bacillus pumilus Y8A which is preserved by China Center for Type Culture Collection (CCTCC) in Wuhan University with the preservation number of CCTCCM 209108. The invention also discloses an application of the lipopeptide-producing bacillus pumilus in promoting the micro-biological degradation process of petroleum and / or cyhalothrin pesticide. Bacterial strain Y8A of the invention can produce lipopeptide biosurfactant with high efficiency and stability, thereby effectively promoting the micro-biological degradation process of petroleum and / or cyhalothrin pesticide degrading bacteria. Bacterial strain Y8A with the function of efficiently producing lipopeptide is expected to work as a new-generation bacteria and cosolvent for antibiotic production and, is widely used in agriculture, medicine, cosmetic and food industries, and can be used for restoration of pollutant environment together with other organic pollution degrading microbes. The bacterial strain is resistant to a plurality of antibiotics with easy fermentation course and convenient operation.

Improvements in plasmid DNA production technology are needed to insure the economic feasibility of future DNA vaccines and DNA therapeutics. General methods are described, by means of which it is possible to dramatically increase plasmid DNA productivity in a fermentor. These processes feature Fed-batch fermentation strategies, combined with novel growth and induction phase temperature shifts.