11results about How to "The fermentation process is simple" patented technology

The application discloses a kind of fermented feed fermented by three strains of probiotics of Bacillus YA ( Bacillus sp ), yeast Y ( Saccharomyces cerevisiae ) and capsular red bacteria R3 ( Rhodobacter capsulatus ), and preparation method and application in aquaculture thereof, and viable count is not less than 1×10 7 CFU / g.The fermented feed is replaced ordinary feed according to certain proportion, and feeding result shows that aquaculture water quality is significantly improved after adding fermented feed, and fish growth performance, digestive enzyme activity, immunity, and muscle quality are also obviously improved.Using the feed in suitable range can shorten growth cycle, reduce cost, improve aquaculture environment, reduce antibiotic use, and improve muscle quality of farmed animals, with good economic and ecological benefits, simple fermentation process, low cost, and suitable for industrial large-scale production.

This invention relates to a spatial multi-layer ultra-high pressure belt dewatering system and method, comprising a main frame, a feeding device, a feeding device, a filter belt, a filter belt traction machine, a filter press hydraulic press, a hydraulic station, and a controller. The feeding device, feeding device, filter belt traction machine, and filter press hydraulic press are connected and installed on the main frame; the output end of the feeding device is connected and installed above the feeding device. The dewatering method includes starting the equipment, feeding, filter press dewatering, and unloading. This invention offers higher dewatering efficiency and lower cost, making it a more effective organic waste dewatering device for reducing pollution and carbon emissions.

The utility model discloses a kind of citric acid fermentation fine filter devices, belong to citric acid production technical field.Device is composed of fermentation unit and fine filter unit: fermentation unit contains seed tank and fermentation tank, the feed inlet of seed tank is connected with syrup turbid liquid pipe, the feed inlet of fermentation tank is connected with syrup clear liquid pipe and syrup turbid liquid pipe, seed tank and fermentation tank are configured with steam, compressed air, defoaming agent, emptying and circulating water pipeline.Fine filter unit first removes most solid impurities with primary plate-and-frame filtration, then ceramic membrane fine filtration and anion and cation exchange impurity removal.Two-stage filter-pressing acid residue is dried after being thickened and then filter-pressing to be feed, and two-stage filtrate respectively enters subsequent process.The device can greatly reduce labor cost and failure rate, improve citric acid purity and yield, and meet the needs of large-scale, high-quality and green production.

This invention belongs to the field of biotechnology and discloses a strain of *Pseudomonas oxysporum* and its application in alleviating continuous cropping obstacles in plants. The preservation number of *Pseudomonas oxysporum* is CCTCC NO: M2023054. This invention isolated a strain of *Pseudomonas oxysporum* RH60 from soil in a cucumber-pepper rotation system. This strain can effectively solve the problem of aggravated root-knot nematode disease under continuous cucumber cropping conditions and efficiently degrades the autotoxic substance p-hydroxybenzoic acid produced by continuous cucumber cropping, while also promoting plant growth. The *Pseudomonas oxysporum* provided by this invention has potential application prospects in the prevention and control of root-knot nematode disease and in solving continuous cropping obstacles caused by autotoxic substances.

The invention discloses a biosynthesis method of alpha-lipoic acid, an engineering strain and a preparation method of the engineering strain. The construction of the engineering strain comprises the following steps: transforming lipocaprylamide enzyme Lpa of enterococcus faecalis, constructing the lipocaprylamide enzyme Lpa to an arabinose promoter or a Cold temperature-sensitive promoter, and then replacing the lipocaprylamide enzyme Lpa to a LacZ gene and an lpxM gene of escherichia coli through RED recombination; or the genes of LipD, lplA, lipA and lpA which are subjected to gene code modification are expressed in the saccharomyces cerevisiae through a pESC vector. The engineering strain is cultured to biologically synthesize alpha-lipoic acid, so that the fermentation cost is further reduced, cyclic synthesis is realized, and the yield is greatly increased.

The invention discloses a method for preparing ethanol through detoxification-free fermentation of furfural residues and application. The method comprises the following steps: S1, carrying out high-solid-content enzymolysis on acidic furfural residues which are not subjected to any detoxification pretreatment to obtain furfural residue enzymatic hydrolysate; s2, liquefying and saccharifying the waste starch to obtain a starch saccharification liquid; and S3, mixing the furfural residue enzymatic hydrolysate and the starch saccharification liquid in proportion, inoculating zymomonas mobilis, and carrying out co-fermentation to produce ethanol. According to the method, key technologies such as detoxification-free treatment, high-solid-content enzymolysis and co-fermentation with starch saccharification liquid are combined, and the steps of neutralization, water washing and deep detoxification which are necessary in a traditional process are thoroughly abandoned, so that the process is greatly simplified, water resources are saved, the treatment cost is reduced, and the problem of toxicity of inhibitors in raw materials is effectively solved. The method provides an efficient, economical and environment-friendly brand-new way for realizing high-valued resource utilization of the furfural residues and the waste starch.

This invention discloses a method for preparing a mulberry-monk fruit fermented beverage, belonging to the field of microbial fermentation technology. The method includes raw material processing, alcoholic fermentation, acetic acid fermentation, and blending steps: Monk fruit is extracted using a combination of juice extraction and hot water countercurrent extraction; the extract is kept warm to improve clarity and inactivate enzymes; using mulberry juice and monk fruit juice as substrates, fruit wine yeast is inoculated for anaerobic fermentation; then, monk fruit juice and edible alcohol culture are added to adjust the alcohol content, followed by aerobic fermentation with *Acetobacter acetosporum* and acetic acid bacteria; finally, the fermented juice, glycoside-containing monk fruit juice, mulberry concentrate, and erythritol are blended to prepare the beverage. This invention fully utilizes mulberry and monk fruit resources, retains active ingredients such as polyphenols, anthocyanins, and polysaccharides, and produces a low-calorie, flavorful, and safe product suitable for industrial production, possessing both nutritional and health benefits.

This invention discloses a bifunctional engineered magnetotactic bacterium. The engineered magnetotactic bacterium is constructed using a recombinant PmamC-MamC-(Gly₄Ser)₃-MagLov fusion gene fragment to construct the expression plasmid pBBR1-MagLov-MamC. Using Magnetospirillum gryphiswaldense MSR-1 as a host, the recombinant expression plasmid pBBR1-MagLov-MamC is conjugated to obtain the bifunctional engineered magnetotactic bacterium MSR-1-MagLov capable of expressing the MagLov-MamC fusion protein. This invention also discloses the construction method and its applications. This invention overcomes the technical bias of exogenous proteins inhibiting magnetosome synthesis, achieving the integration of magnetotactic motility and magnetosensitive fluorescence.

The invention belongs to the technical field of microorganisms, and discloses an African trichoderma harzianum, a biocontrol inoculant and application of the African trichoderma harzianum, the African trichoderma harzianum is named as African trichoderma harzianum T10, the preservation number of the African trichoderma harzianum is CGMCC No.41709, the African trichoderma harzianum is preserved in China General Microbiological Culture Collection Center on December 11, 2024, and the preservation number of the African trichoderma harzianum is CGMCC No.41709. The preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The active ingredient of the biocontrol fungicide is the African trichoderma harzianum. The African trichoderma harzianum T10 has an inhibiting effect on apple tree canker and apple ring spot, can be used for preparing a biocontrol microbial inoculum, is applied to prevention and treatment of the apple tree canker and the apple ring spot, also has a growth promoting effect on fruit trees, can be used as an active component to prepare a plant growth promoting agent, and also can be applied to degradation of canker toxin. The African trichoderma harzianum T10 has the advantages of stable control effect and environmental friendliness.

The invention belongs to the technical field of bioengineering, and relates to a recombinant engineering bacterium and application thereof in preparation of mannitol, and the recombinant engineering bacterium is a plasmid-free strain for producing a cofactor NAD < + > by using cheap xylose and nicotinamide. The following genes are knocked out from the strain: a gene ptsG for coding protein EIICBGlc in a glucose PTS system, mtlA for coding EIICA protein in D-mannitol transmembrane transport, a D-mannitol 1-phosphate dehydrogenase gene mtlD in a D-mannitol utilization path, purine de novo synthesis regulation protein purR, and srlAE in a sorbitol utilization gene cluster. The following genes are integrated in a genome of the strain: glucose transport assisting protein glf, glucokinase glcK, xylose utilization gene cluster xylose isomerase xlyA and xylulose phosphorylase xlyB, phosphoribose pyrophosphate synthetase prs, polyphosphate kinase ppk2, nicotinamide phosphoribose transferase nadV and nicotinamide mononucleotide adenylate transferase nadM. The strain is fermented by using glucose and xylose, and provides chassis bacteria for reaction needing cofactor circulation.

The present application relates to a kind of fermentation medium of mupirocin and the production method of mupirocin.The present application improves fermentation medium and the production method of mupirocin, significantly improves the fermentation level of mupirocin, and when fermenting 5-7 days, the fermentation titer reaches 12000mg / L or more.The whole production process is cheap and easy to obtain, and the fermentation process is simple and easy to operate, which is beneficial to industrial production.