Method for synthesizing biological plastic precursor polyhydroxyalkanoate by utilizing lignin degrading bacterium

A technology of polyhydroxyalkanoate and lignin degrading bacteria is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of reducing carbon source and its pretreatment cost, and simple fermentation conditions and process.

Active Publication Date: 2016-12-07
CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Alkali lignin carbon source without any pretreatment

Method used

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  • Method for synthesizing biological plastic precursor polyhydroxyalkanoate by utilizing lignin degrading bacterium
  • Method for synthesizing biological plastic precursor polyhydroxyalkanoate by utilizing lignin degrading bacterium
  • Method for synthesizing biological plastic precursor polyhydroxyalkanoate by utilizing lignin degrading bacterium

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Embodiment 1

[0023] In an L-test tube, add 5 mL of LB medium, insert a single colony of the Cupriavidus basilensis B-8 strain, and cultivate and grow at 30° C. and 150 rpm until the optical density of the bacteria at 600 nm reaches 1.0. The pre-culture was centrifuged, washed, and then inoculated into Erlenmeyer flasks containing 100 mL of different concentrations of alkali lignin medium (1-6 g / L), and cultured with shaking at 30 °C and 150 rpm for 48 h. Among them, the medium preparation method: alkali lignin 1 ~ 6g, (NH 4 ) 2 SO 4 0.28g, K 2 HPO 4 1g, MgSO 4 0.2g, CaCl 2 0.1g, FeSO 4 0.05g, MnSO 4 0.02g, KH 2 PO 4 1g, agar 15g, distilled water 1000mL, pH 7.0~7.4.

[0024] Collect 2 mL of sample, discard the supernatant, and resuspend the cells in 150 μL of deionized water and 50 μL of dimethyl sulfoxide. 5 μL of Nile Red (0.15 mg / mL) was added to the suspension for staining for 30 min. The results were then observed with a fluorescence microscope. figure 1 As shown, i...

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Abstract

The invention discloses a purpose of lignin degrading bacterium, and particularly relates to a method for synthesizing a biological plastic precursor-polyhydroxyalkanoate by utilizing lignin degrading bacterium (Cupriavidus basilensis B-8) with a collection number of CGMCC (China General Microbiological Culture Collection Center) No. 4240. A bacterial strain can grow by utilizing lignin (the lignin concentration of a growth medium is 1g/L to 6g/L) which is not subjected to pretreatment as a unique carbon source, and is used for synthesizing a homopolymer of biological plastic-polyhydroxyalkanoate in the condition (the concentration of nitrogen is less than 60mg/L) that a nitrogen source is short. By using a culture and a production method which are provided by the invention, the carbon source and the pretreatment cost of the carbon source are greatly decreased; the method has large-scale production and industrialization potential.

Description

technical field [0001] The invention belongs to the technical field of preparing polyhydroxyalkanoate by microorganisms, relates to the use of a lignin-degrading bacterium, and particularly relates to a method for synthesizing a bioplastic precursor-polyhydroxyalkanoate by using the lignin-degrading bacterium. Background technique [0002] Petrochemical plastics have become one of the most widely used materials by humans. In 2010, global plastic production reached nearly 3 × 10 8 t, is expected to reach nearly 4 × 10 in 2050 8 t. However, the international oil reserves are decreasing year by year, and the environmental pollution of petrochemical plastics is gradually increasing. Therefore, it is urgent to develop alternatives to new petrochemical plastics. Polyhydroxyalkanoate has similar mechanical properties to traditional plastics, can be completely degraded by microorganisms and enter the natural ecological cycle, and is considered to be an important substitute for p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/62C12R1/01
CPCC12P7/625C12P2203/00C12N1/205C12R2001/01
Inventor 柴立元石岩颜旭刘明人杨志辉
Owner CENT SOUTH UNIV
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