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Technology for preparing docosahexaenoic acid (DHA)-rich feed additive by grain dreg raw material through fermentation

A technology for additives and slag, which is applied in the technological field of producing DHA-rich feed additives by using slag raw materials by fermentation, can solve the problem of high protein treatment and other problems, and achieves the advantages of reducing the cost of carbon and nitrogen sources and reducing consumption. Effect

Active Publication Date: 2015-01-07
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method only solves the problem of degradation of lignocellulosic materials, and does not solve the problem of processing waste residues with high protein, high starch and high water content.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Liquor distiller's grains are by-products of the winemaking industry. According to statistics, my country's annual production of liquor in 2013 was 10 million tons, and liquor lees reached more than 30 million tons. If it is not dealt with in time, it will become corrupt and deteriorate, which will not only waste precious resources, but also seriously pollute the surrounding environment.

[0028] Take 100g of fresh liquor distiller's grains with a water content of 70%, add 20ml of water, stir and mix well, and make a mixed material with a solid-liquid mass ratio of 1:3 and a pH value of 3. The mixed material was placed in a hydrothermal reactor with a stirring speed of 200 rpm, heated at 180° C., and maintained at this temperature for 10 minutes. After the treatment was completed, the heating was stopped and the temperature was rapidly lowered to 55°C. Adjust the pH value to 3, add glucoamylase with a total activity of 3000AGU, xylanase with 20000IU, and cellulase wit...

Embodiment 2

[0030] Take 100g of fresh vinegar grains with a water content of 90%, add 410ml of water, stir and mix well, and make a mixed material with a solid-liquid mass ratio of 1:50 and a pH value of 8. The mixed material was placed in a hydrothermal reactor with a stirring speed of 300 rpm, heated to 230° C., and maintained at this temperature for 1 minute. After the treatment was completed, the heating was stopped and the temperature was rapidly lowered to 80°C. Adjust the temperature to 90° C. and pH to 8, add glucoamylase with a total activity of 1 AGU, 1000 IU of xylanase, and 1000 FPU of cellulase to react for 48 hours. After 48 hours, add 1000 LAPU of acid protease and act for 2 hours. After filtering with 200-mesh gauze, take the filtrate to measure the glucose concentration and ammonia ion concentration. The filtrate glucose concentration was adjusted with glucose and water. The main components of the fermentation medium are glucose 60g / L, yeast extract 10g / L, potassium di...

Embodiment 3

[0032] Take 100g of cornstarch residue with a water content of 80%, add 420ml of water, stir and mix thoroughly, and make a mixed material with a solid-liquid mass ratio of 1:25 and a pH value of 5. The mixed material was placed in a hydrothermal reactor with a stirring speed of 150 rpm, heated to 120° C., and maintained at this temperature for 180 minutes. After the treatment was completed, the heating was stopped and the temperature was rapidly lowered to 20°C. Adjust the pH value to 5, add glucoamylase with a total activity of 1000AGU, xylanase with 2500IU, and cellulase with 2FPU to react for 68 hours. After 68 hours, add 0.002 LAPU of acid protease and act for 24 hours. After filtering with 200-mesh gauze, take the filtrate to measure the glucose concentration and ammonia ion concentration. The filtrate glucose concentration was adjusted with glucose and water. The main components of the fermentation medium are glucose 60g / L, yeast extract 10g / L, potassium dihydrogen p...

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Abstract

The invention discloses a technological method for preparing an animal feed additive which is rich in polyunsaturated fatty acid, namely, docosahexaenoic acid (DHA), protein and xylo-oligosaccharide by taking a grain dreg raw material as a culture medium through a microbiological fermentation method. The method concretely comprises the steps of (1) mixing the grain dreg raw material with water to prepare a material with a certain solid-liquid mass ratio; (2) carrying out pretreatment on the mixed grain dreg raw material by a hydrothermal extraction method; (3) carrying out enzymolysis on the pretreated grain dregs; (4) culturing microorganism capable of producing DHA by the grain dregs treated by enzymolysis; and (5) concentrating or drying fermentation liquor of schizochytrium limacinum to prepare a liquid or solid-state feed. The method fully utilizes the principle that the grain dregs contain rich hemicellulose and cellulose so as to be used for preparing xylo-oligosaccharide and cello-oligosaccharide with a probiotic function, and soluble lignin is used for preparing a polyphenol antioxidant; and the degraded cellulose and starch can be used as a carbon source for microbial growth, and the protein can be used as a nitrogen source for cell growth, so that the costs of the carbon source and the nitrogen source for microbial fermentation can be lowered.

Description

technical field [0001] The invention relates to a production process of a feed additive, in particular to a process for producing a DHA-rich feed additive by using dross materials as raw materials. Background technique [0002] DHA (docosahexaenoic acid) is an extremely important highly unsaturated fatty acid, which is an important component of human cell membranes and nerve tissues. DHA plays an important role in preventing cardiovascular diseases, inhibiting and treating cancer, ensuring the normal development of the fetus and promoting the development and growth of infants and young children. However, the daily DHA intake of Chinese people from food is only 40mg, while the recommended minimum standard intake is 160mg), which is a serious deficiency. Invention patent 201210385417.5 discloses "a microalgae powder rich in DHA and its preparation method". In this patent, the fermented liquid rich in DHA is directly processed into microalgae powder, which is then used as a fe...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/06A23K1/00A23K10/38
CPCY02P60/87
Inventor 崔球孙志杰王立英
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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