96results about How to "Improve enzymatic hydrolysis rate" patented technology

The invention discloses a technological method for preparing an animal feed additive which is rich in polyunsaturated fatty acid, namely, docosahexaenoic acid (DHA), protein and xylo-oligosaccharide by taking a grain dreg raw material as a culture medium through a microbiological fermentation method. The method concretely comprises the steps of (1) mixing the grain dreg raw material with water to prepare a material with a certain solid-liquid mass ratio; (2) carrying out pretreatment on the mixed grain dreg raw material by a hydrothermal extraction method; (3) carrying out enzymolysis on the pretreated grain dregs; (4) culturing microorganism capable of producing DHA by the grain dregs treated by enzymolysis; and (5) concentrating or drying fermentation liquor of schizochytrium limacinum to prepare a liquid or solid-state feed. The method fully utilizes the principle that the grain dregs contain rich hemicellulose and cellulose so as to be used for preparing xylo-oligosaccharide and cello-oligosaccharide with a probiotic function, and soluble lignin is used for preparing a polyphenol antioxidant; and the degraded cellulose and starch can be used as a carbon source for microbial growth, and the protein can be used as a nitrogen source for cell growth, so that the costs of the carbon source and the nitrogen source for microbial fermentation can be lowered.

The invention discloses a beef flavour powder and a preparation method thereof. The beef flavour powder comprises: dust salt, white granulated sugar powder, glucose powder, gourmet powder, D- xylose, L-cysteine, enzymolysis solution, soy sauce, yeast extract, sesame oil, phosphoric acid, ethyl maltol, IMP, corn starch and beef flavor. The invention adopts the method combining microwave drying technology and compound enzyme enzymolysis technology to produce the beef flavour powder which has good taste, little environmental pollution, stable quality and high safety.

The invention provides a method for removing aflatoxin out peanut oil. The method comprises the following steps: A, selecting materials; B, grading and screening peanuts; and C, feeding and squeezing oil, The method is characterized in that aflatoxin is removed by means of a compound enzyme in the step C, wherein the compound enzyme consists of phospholipase A1, laccase, neutral proteases, xylanases by adding phospholipase A1, laccase, neutral proteases and xylanases respectively according to proportions of 0.3g/kg, 0.2g/kg, 0.5g/kg and 0.1g/kg; then hydrolysis is carried out at 42 DEG C for 5-6 hours; and finally the pH of hydrolyzate is adjusted to 6.8. According to the method, aflatoxin is removed by a biological method, reaction conditions are mild and operation is simple, aflatoxin content meets national standards, and the method disclosed by the invention has a broad application prospects.

The invention relates to a lignocellulose pretreatment method for increasing an enzyme hydrolysis rate by oxidation removal of lignin. The method includes steps: grinding and drying lignocellulose raw materials, respectively controlling sodium percarbonate concentration, solid-liquid ratio, pretreatment temperature and time in appropriate ranges, filtering, washing to neutral, and drying to obtain pretreated lignocellulose residues; adding cellulase and xylanase, dissolved in citric acid buffer solution with pH in a range of 4.5-5.0, into the pretreated lignocellulose residues to obtain fermentable reducing sugar solution through enzymolysis. By the pretreatment method, lignin can be effectively removed while the retaining rate of cellulose and hemicellulose components is high, lignocellulose enzymolysis efficiency is effectively improved, and reducing sugar yield is increased. In addition, the method has advantages of environmental friendliness, simplicity in operation, low cost and the like.

The invention belongs to a method for producing ethanol from tobacco stems by fermentation; a fibrous raw material is processed under the action of high-temperature and high-pressure steam by adopting a steam explosion method; the component separation of the raw material is realized through a sudden depressurizing process; therefore, cellulose, hemicellulose and lignin in the material are degraded and dissolved out; cellulose is easily subjected to emzymolysis so as to be converted into sugar; and then, fermentation of cellulosic ethanol is carried out by selectively using an issatchenkia orientalis strain. The pre-processing method can be applied to various agricultural wastes, such as bagasse, wheat straws, rice straws, corn stalks and corncobs; the enzymatic hydrolysis ratio of cellulose can be obviously increased; ethanol is prepared by fermentation by taking tobacco wastes as the raw materials; therefore, the operation process is simple; the production cost is low; furthermore, the agricultural wastes can be changed into wealth; environmental pollution is reduced; and reasonable development and utilization of agricultural surplus resources are realized.

The invention discloses a composite enzymolysis reagent for keratin enzymolysis and an application method thereof. The composite enzymolysis reagent comprises streptomycete cutin protease, yeast enzyme and a reducing agent. The application method comprises the following steps: firstly, performing ungrease treatment on a natural raw material with cornifin, a yeast enzyme and a reducing agent; adding the natural raw material with the cornifin after ungrease treatment in the step 1) into a combined enzymolysis agent liquid, pretreating for 15-25 minutes at the room temperature without oxygen, further adjusting the pH value of the combined enzymolysis agent liquid to be 7.0-7.5, controlling the temperature to be 50-60 DEG C, and performing enzymolysis for 12-24 hours, thereby obtaining a keratinase liquid; decoloring and sterilizing the keratinase liquid; and performing vacuum drying, thereby obtaining protein powder. The composite enzymolysis reagent is rapid in enzymolysis and high in keratin degradation rate, and generation of toxic substances can be effectively prevented, so that the prepared protein powder is safe and non-toxic and high in nutrition component.

The invention discloses a preparation method of composite amino acid chelated calcium. The method adopts scallop outer mantles and scallop shells which are leftovers of scallop processing as raw materials, fully utilizes the characteristics that the scallop outer mantles are high in crude protein content and the scallop shells are high in calcium carbonate content, and subjects the scallop outer mantles to cooking, degreasing, enzymolysis and chelation with calcium salt so as to obtain an organic calcium product rich in a plurality of amino acids. Not only the method fully utilizes the leftovers of scallop processing and reduces production cost, but also the prepared composite amino acid chelated calcium has high nutritional value and a delicious taste so as to bring great economic value.

The invention provides a device and method for performing continuous pretreatment on lignocellulose material by utilizing microwave-diluted acid steam explosion and relates to the field of biomass pretreatment. The lignocellulose material is conveyed into a feeder, and diluted acid in a diluted acid tank is continuously sprayed to the material through a spraying valve to be steeped. The material enters an anti-back spraying pressurization screw device and undergoes kneading, extrusion dehydration, pressurization and accelerated steeping in the anti-back spraying pressurization screw device and then enters a reaction retention device; in the reaction retention device, the material undergoes combined effects of microwaves and saturated steam and performs spray explosion on a cyclone separator through a spray explosion valve, and a solid product is separated. The lignocellulose undergoes continuous treatment of combination of microwave pretreatment and diluted acid steam explosion pretreatment for the first time, and the device capable of implementing the process smoothly is designed. According to the method, the lignocellulose treatment amount is improved, the treatment cycle is shortened, the energy consumption is reduced, the enzymatic accessibility of the material after pretreatment is improved, and the cellulose enzymolysis rate is improved.

The invention provides an aflatoxin-effectively-removing peanut oil extracting method .The aflatoxin-effectively-removing peanut oil extracting method includes the following steps of pretreating, crude oil preparing, degumming, alkali refining, primary evaporating and separating of a solvent, secondary evaporating and separating of a solvent, dewaxing and deodorizing .By means of the aflatoxin-effectively-removing peanut oil extracting method, pigment and waxiness in grease are effectively removed, removing of aspergillus flavus and producing of peanut oil are combined accordingly, production of the peanut oil is not influenced, the content of the aspergillus flavus is greatly decreased, invariable nutrient substance ingredients are reserved through the produced peanut oil, taste is pure, fragrance is lasting, the requirements of persons for original-taste-and-flavor superfine peanut oil can be met, the reaction condition is wild, operation is easy, and good application prospects are achieved.

The invention discloses a making method of 5'-nucleic acid through enzymolyzing RNA by composite phospho-esterase of malt root, which is characterized by the following: adopting liquid RNA as substrate; inhibiting the activity of phosphomonoesterase and 3'-phosphodiesterase through the activity of composite phospho-esterase; obtaining the production of 5'-nucleic acid more than 15mg/ml.

The invention discloses a pretreatment method for lignocellulosic materials. The method is as follows: a high-pressure homogenizer which is conventional but is currently only used for processing liquid state materials is taken as the treatment equipment, a stirring device is installed at the feed funnel of the homogenizer, the materials are exploded under ultrahigh pressure by means of transient pressure and pressure elimination in the homogenizing process. The method comprises the steps as follows: the pre-processes like drying, smashing and sieving, etc. of the lignocellulosic materials, the heating and moistening of the materials, the preparation of the suspending liquid, the ultrahigh pressure explosion and the material process after explosion, etc. Compared with various currently disclosed pretreatment methods for lignocellulosic materials, the method not only has the advantages of low requirement for equipment, mild process condition, no harmful substances which inhibit microorganism fermentation and low energy consumption, etc. but also can process the ultramicrostructure which totally changes the lignocellulosic materials and creatively realize the full hydrolyzation of the lignocellulosic materials by making use of the cellulolytic enzyme system of low beta-glucosidase activity.

The invention discloses a chicken flavour powder and a preparation method thereof. The chicken flavour powder comprises dust salt, white granulated sugar powder, glucose powder, gourmet powder, D- xylose, isoleucine, enzymolysis solution, soy sauce, yeast extract, sesame oil, chicken oil, ethyl maltol, IMP, corn starch and chicken flavor. The invention adopts the method combining the microwave drying technology and compound enzyme enzymolysis technology to produce the chicken flavour powder which has good taste, little environmental pollution, stable quality and high safety.

The invention discloses a chicken flavor material and a preparation method of the chicken flavor material. The chicken flavor material comprises the components of chicken, a chicken bone, an egg, chicken fat, water, protease, compound flavor protease, glucose, white sugar, xylose, vitamin B1, L-cysteine, methionine, salt, aginomoto, corn starch and maltodextrin. The prepared chicken flavor material keeps the original taste and flavor of the natural chicken to the highest extent, the delicate mellow flavor is greatly improved, the taste is real, the aftertaste lasts long, the chicken flavor material is excellent in heat stability, only 10% of the chicken flavor material is denatured after being sterilized at the temperature of 121 DEG C, and the technological requirements in thermal treatment in the food industry are met.

The invention discloses a water body remediation wood fiber raw material pretreatment method sequentially comprising the following steps: (1) coarsely grinding, that is, coarsely grinding a water body remediation wood fiber raw material after air drying; (2) carrying out steam explosion, that is, carrying out steam explosion pretreatment on obtained sieving powder; (3) dissolving with an ionic liquid and regenerating a material, that is, adding the ionic liquid to a steam explosion material for dissolution treatment, and separating out solid from the steam explosion material after dissolution treatment by using water as an antisolvent; and (4) carrying out enzymatic saccharification, that is, carrying out enzymolysis by using the steam explosion material obtained from the step (2) or regeneration material obtained from the step (3) as a substrate to obtain an enzymatic saccharification liquid. The invention also provides a method for carrying out fermentation hydrogen production by utilizing the enzymatic saccharification liquid obtained from the method, that is, applying the enzymatic saccharification liquid to biological hydrogen production.

The invention discloses a method for treating straws through dissolution and regeneration. The method comprises the following steps: firstly, conducting pretreatment on pulverized straws through an ethanol sulfate water solution to remove hemicellulose and lignose; then drying pretreated straws and activating the dried straws through an organic solvent; secondly dissolving the activated straws into lithium chloride/N,N-dimethylacetamide to obtain a straw solution; thirdly injecting the straw solution into water for regeneration to obtain regenerated straws; finally conducting water washing on the regenerated straws to remove the organic solvent and saline ions, and conducting enzymolysis by adopting cellulose complex enzyme. The straw treating method is simple, and the enzymolysis rates of cellulose and hemicellulose in the straws are greatly improved.

The invention provides a method for preparing fumaric acid fermentation liquor by fermenting corncob cellulose. After corncob is pretreated by using alkaline hydrogen peroxide, lignin and hemicellulose in the corncob can be removed effectively, the purity of the cellulose is increased, the cellulose is loose in structure and easier to hydrolyze, and the loss ratio of the corncob is reduced to 26.85-27.16 percent. On the basis, the pretreated corncob raw material is subjected to pre-enzymolysis, a part of the cellulose in the raw material is converted into small-molecular sugar, a rhizopus delemar seed solution is introduced, enzymolysis and fermentation are performed synchronously, glucose is converted into fumaric acid continuously in an enzymolysis process, the feedback inhibition action of a large amount accumulated glucose on enzyme in a subsequent enzymolysis process is relieved, and the cellulose conversion rate and the fumaric acid yield are increased. According to the method, the corncob is taken as a raw material, so that a novel way is opened up for the production of the fumaric acid, and the economical significance of industrial microorganism production is great.

The invention provides a refining method for removing aflatoxin in corn oil.The refining method comprises the following steps of pretreatment, crude oil preparation, degumming alkali refining, primary vaporization for separating solvent, secondary vaporization for separating solvent, dewaxing and deodorization.By means of the method, pigment and waxiness in grease can be effectively removed, the solvent residue is reduced, aspergillus flavus removing and corn oil producing are combined, corn oil production is not affected, the content of aflatoxin is greatly reduced, the components of nutrient substances of the produced corn oil are not changed, taste is pure, fragrance is lasting, the requirement for special-grade corn oil with original taste and flavor of people can be met, reaction conditions are mild, operation is easy, and the method has good application prospects.