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Method for producing hemepeptide by using red blood cells of animal blood as raw materials

A technology of red blood cells and red blood cells, which is applied in the field of preparation of blood peptides, can solve problems such as affecting single use and unsatisfactory yields, and achieves low production costs, improved enzymatic hydrolysis efficiency and yield, and improved enzymatic hydrolysis rate.

Inactive Publication Date: 2012-02-08
赵运生 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the above-mentioned public reports, it can be known that the yield of hydrolysis of the existing animal red blood cells is not ideal, all less than 60%.
In addition, since the hydrolyzed products of animal blood (especially poultry blood) have a special fishy smell, it is difficult to use them alone or add them to other foods.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 1kg of red blood cells, add 2kg of distilled water, stir for 1.5h, heat up to 72°C under stirring, and keep for 20min for thermal denaturation, then cool down to 42°C, adjust the pH to 7.5 with NaOH (20%, w / w), press 1200U Add hemocytometer hydrolysis enzyme 1122 at a ratio of 1 g protein, stir for 4.5 hours (keep at 42°C throughout the enzymolysis process), heat up to 85°C to inactivate the enzyme for 15 minutes, filter to obtain 2600 g of filtrate, add 0.15% of its weight to the filtrate % deodorizing agent, stirred and heated to 65°C, added activated carbon equivalent to 1.5% of the weight of the filtrate, continued to stir for 30 minutes, filtered, the obtained decolorized solution was concentrated by rotary evaporation to a solid content of 35%, and spray-dried to obtain blood peptide powder 248.5g, yield 78.9%.

Embodiment 2

[0026] Take 100kg of red blood cells, add 300kg of distilled water, stir for 2 hours, heat up to 78°C under stirring, keep for 30min for thermal denaturation, then cool down to 45°C, adjust the pH to 8.0 with NaOH (20%, w / w), press 1600U / Add hemocytometer hydrolysis special enzyme 1122 to the proportion of g protein, stir for 5.5 hours (keep at 45°C during the whole enzymolysis process), heat up to 90°C to inactivate the enzyme for 20 minutes, filter to obtain 265.4 kg of filtrate, add 0.17 kg of its weight to the filtrate % deodorizing agent, stirred and heated to 70°C, added activated carbon equivalent to 1% of the weight of the filtrate, continued to stir for 40 minutes, filtered, the obtained decolorized solution was evaporated under reduced pressure to a solid content of 45%, spray-dried to obtain blood peptide Powder 25.64kg, yield 81.4%.

Embodiment 3

[0028] Take 1000kg of red blood cells, add 4000kg of distilled water, stir for 2 hours, heat up to 82°C under stirring, keep for 40min for thermal denaturation, then cool down to 50°C, and use Na 2 CO 3 (20%, w / w) to adjust its pH to 8.5, add hemocyte hydrolysis enzyme 1122 at a ratio of 1900 U / g protein, stir for 6.5 hours (keep at 50°C throughout the enzymolysis process), heat up to 95°C to inactivate the enzyme for 30 minutes , filter to get 4680kg of filtrate, add fishy smell agent equivalent to 0.2% of its weight in the filtrate, stir and heat to 75°C, add activated carbon equivalent to 1.5% of the weight of the filtrate, continue stirring for 50min, filter, and the decolorized liquid obtained is decompressed Evaporated and concentrated to a solid content of 50%, and then spray-dried to obtain 254.9 kg of blood peptide powder, with a yield of 80.92%.

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Abstract

The invention discloses a method for producing hemepeptide by using red blood cells of animal blood as raw materials. The method comprises the following steps of: adding water into the red blood cells of the animal blood, stirring for wall breakage, heating, performing thermal denaturation, adjusting the pH of the obtained mixture to be between 7.5 and 9.0 at the temperature of between 40 and 60 DEG C, adding special enzyme 1122 for blood cell hydrolysis in a ratio of 1,000 to 2,500U / (g protein), performing enzymolysis for 4 to 8 hours with stirring, inactivating the enzyme, filtering, concentrating the filtrate, performing spray drying, and thus obtaining the hemepeptide. According to the method, the red blood cells are subjected to wall breakage and thermal denaturation, so that the double-spiral structure of the red blood cell protein becomes loose, the special enzyme 1122 for blood cell hydrolysis enters the amino acid binding sites in the red blood cell protein more easily to perform enzymolysis, and the enzymolysis rate is improved; and the adopted special enzyme 1122 for blood cell hydrolysis can be used for simultaneous enzymolysis of multiple amino acid binding sites of the red blood cell protein, so that the enzymolysis efficiency and the yield are further improved, and the yield of the whole hydrolysis process reaches over 78 percent.

Description

technical field [0001] The invention relates to a preparation method of blood peptide, in particular to a method for producing blood peptide using red blood cells of livestock and poultry as raw materials. Background technique [0002] Animal blood is a valuable nutritional resource, rich in more than 200 protein components (16.4%-18.9%) with different functions, including eight kinds of amino acids necessary for the human body (the content of lysine and leucine is particularly high), and There are calcium, iron, magnesium, phosphorus, potassium and other trace elements, vitamins, inorganic salts, sugars and lipids. [0003] my country's animal blood resources are extremely rich, estimated at 2.3×10 6 Some of them are simply processed into blood meal as a feed additive, and most of the blood is discarded, and only a small part is developed and eaten. The existing development of animal blood is mostly to hydrolyze the erythrocytes of animal blood to obtain available product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/06
Inventor 赵运生
Owner 赵运生
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