966results about How to "Improve enzymatic hydrolysis efficiency" patented technology

The invention relates to a maize oligopeptide and a method for preparing same, belonging to the field of biological technology. The method takes the byproduct, namely corn protein powder (also called maize yellow powder containing about 60% of protein) in corn starch production as a raw material, removes the impurities such as starch, grease and the like by the treatment of alkaline water and obtains high-purity corn protein slag; furthermore, the corn protein slag is taken as enzymatic hydrolysis substrate and treated by the following process of pre-treatment, enzymatic hydrolysis, multi-step separation and purification, concentrating, drying and the like so as to prepare the maize oligopeptide powders with edible value and various health-care functions. The method for preparing maize oligopeptide has the advantages of easily obtaining raw material, simple process and higher industrial feasibility. The prepared maize oligopeptide sample has high crude protein content and small molecular weight, has various physiological activities of prompting immunity adjustment, anti-fatigue and the like, and can be used as new functional nutritional compounding and widely applied to the development and production of products such as foods, health-care foods and the like.

The invention discloses a device and a method for biological processing with pulsed multi-frequency ultrasonic waves in a sweep-frequency mode and use thereof, which relate to the field of agricultural product deep processing. The device consists of an ultrasonic wave processing system, an ultrasonic wave generating system and a computer control system, which are sequentially connected by cables, wherein the ultrasonic wave processing system consists of an upper ultrasonic wave transducer, a lower ultrasonic wave transducer, an ultrasonic wave processing tank body, a circulating pump and an upper oscillatory plate height adjusting screw rod; an ultrasonic wave generator set consists of a group of pulsed ultrasonic wave generators at different frequencies; and the control system consists of an ultrasonic wave controller, a temperature controller and a computer. The equipment can be used in ultrasonic wave-assisted extraction of active ingredients of natural products and the extraction efficiency is improved 10 to 90 percent. The equipment can be used in ultrasonic wave-assisted enzymolysis of biological macromolecules, wherein the ultrasonic wave-assisted enzymolysis includes preprocessing raw materials, preprocessing hydrolases and performing enzymolysis in an ultrasonic wave field; and the enzymolysis efficiency is improved by 25 to 65 percent.

The invention relates to a method for breaking walls of cells of bran by wet grinding, and belongs to the technical field of industrial biochemistry. The invention adopts the technical key point that: bran, water, and enzyme for degrading walls of cells of the bran are mixed, and subjected to wet ball-milling grinding to form superfine wall-breaking powder. By combining an ultramicro-pulverization method, the water and the enzyme for degrading the walls of cells of the bran, the cells of the bran are subjected to wet ball milling for wall-breaking treatment; compared with single ultramicro pulverization, the method can greatly reduce the energy consumption, is simple and has low requirement on operating equipment; and compared with single enzymatic degradation, the method combines wet ball milling, combines chemical action with mechanical force action, and has the advantages of greatly saving energy required by high temperature treatment, avoiding the defect of enzyme inactivation under high temperature treatment, eliminating crystal structures of cellulose and improving enzymolysis efficiency.

The invention relates to a method for pretreating lignocellulose by adopting an alkaline eutectic solvent. With the method, the enzymolysis efficiency of lignocellulose can be effectively enhanced, particularly, the recycling for the hemicellulose sugar monomer is improved, and the yield of total sugar is improved. The method has the advantages that the cost is low, the operability is strong, theenergy consumption is small, the environment protection is realized, etc.

The invention discloses a method for increasing enzymolysis efficiency of rice straws through choline and amino acid ionic liquid pretreatment, which comprises the following steps: firstly, performing pretreatment on the rice straws at certain temperature for a certain period of time by taking choline and amino acid ionic liquid as a solvent; then filtering and separating to obtain residues; drying the residues to obtain the pretreated rice straws. The residues of the rice straws are used as substrates, and enzymolysis is performed on the substrates by utilizing cellulase and xylanase to finally obtain sugar liquor taking glucose and xylose as main components. The method for increasing the enzymolysis efficiency of the rice straws through the choline and amino acid ionic liquid pretreatment not only is capable of effectively increasing the enzymolysis efficiency of the rice straws and increasing the yield of reducing sugar (the glucose and the xylose) which can be fermented, but also is capable of overcoming the defects that a traditional ionic liquid pretreatment process environment is not friendly and the like.

The invention discloses a method for preparing ultra-low molecular weight hyaluronic acid oligosaccharides and a salt thereof through combination of solid-liquid biphasic enzymolysis and ultrafiltration. The method comprises the following steps: degrading hyaluronic acid and a salt thereof by utilizing hyaluronidase produced by bacilli; preparing high-concentration hyaluronic acid enzymatic hydrolysate by adopting the method of combining a solid-liquid biphasic enzymolysis system and an ultrafiltration system, and then performing inactivating, activated carbon adsorption and impurity removal,filtering and spray-drying to obtain the ultra-low molecular weight hyaluronic acid oligosaccharides and the salt thereof with the molecular weight of less than or equal to 3 kDa. The product preparedby the method is high in stability, ultra-low in molecular weight, good in inflammation resistance and percutaneous absorptivity, high in purity, strong in oxidation resistance, does not have cytotoxicity, has the advantages of repairing skin cell damage and the like, and can be widely applied to the fields of cosmetics, food and medicines. According to the method, the process flow is easy to operate; conditions are mild; various alcohols are not used; the energy consumption is relatively low; the method does not have damage to a product structure, does not have environmental pollution, and is suitable for large-scale industrial production.

The invention relates to an in-situ rapid preprocessing method for protein samples. The whole preprocessing process of the protein samples is carried out in a centrifugal ultrafiltration device in situ, the denaturation process, the reduction process, the solvent replacement process, the alkylate process, the enzymolysis process and the impurity removal process of the protein samples are integrated, and enzymolysis of the protein samples is accelerated under assistance of microwaves. The method can remarkably improve the preprocessing flux of the protein samples; meanwhile, the whole preprocessing process of the samples is carried out in situ, and the recovery rate is high. In addition, the centrifugal ultrafiltration device can conveniently and rapidly achieve the purification of the samples and replacement of solvents, and enzymatic hydrolysate does not need an additional purification process and can be directly combined with subsequent efficient liquid chromatogram separation and mass spectrum identification.

The invention relates to a rice bran soluble dietary fiber, a production technology and application thereof, and belongs to the field of comprehensive utilization and deep processing of grains. The production technology comprises the following steps: rice bran steam explosion pretreatment, enzymolysis, extraction, concentration, alcohol precipitation and drying. The obtained dietary fiber has higher water-holding capability, oil-holding capacity and expansive force, improves the physiological activity of reducing blood sugar, reducing cholesterol and oxidation resistance and the like, and can be widely applied to the fields of food, health care products and the like.

The invention discloses a production method for convenient rice with a favorable retrogradation resisting performance, which comprises the steps of raw material rice washing, primary soaking, primary cooking, secondary soaking, secondary cooking, dispersing, drying and packaging, wherein a primary soaking solution is a water solution containing 0.2-0.8 percent by weight of beta-cyclodextrine and 0.1-0.4 percent by weight of monoglyceride; and a secondary soaking solution is a water solution containing 0.2-0.8 percent by weight of beta-cyclodextrine, 0.1-0.4 percent by weight of monoglyceride,1000-8000U/100g of alkali protease of raw material rice and 300-3000U/100g of beta- cyclodextrine of the raw material rice. According to the production method disclosed by the invention, the process is simple, the favorable retrogradation resisting performance of the dehydrated convenient rice can be improved, the mouthfeeling of the rice is improved and the rehydration time of the convenient rice is shortened; and the production method is more convenient to use.

The invention relates to a method for pre-treating lignocellulose raw material and for preparing reducing sugar by conversion. With liquid-solid ratio of 5-50, lignocellulose is treated for 1-10h in a mixed solution of 0-5% of sodium hydroxide by mass and 0-5% of hydrogen peroxide by mass; solid residue obtained by treating is washed by using water, and is dried by baking; the obtained material is treated under a liquid-solid ratio of 5-50, an acid hydrolysis time of 10-120min, an acid hydrolysis temperature of 30-150min, and a sulfuric acid mass concentration of 0-10%, such that a solid residue is obtained; a pH value is regulated to 7; and the obtained material is dried by baking. With the treatment process, lignin can be effectively removed, and hemicellulose is hydrolyzed under a dilute acid condition, such that pentose is obtained. With further reactions, products with high added value can be obtained. With a sample treated with the process, ineffective adsorption upon cellulase is reduced, and a conversion rate of cellulose in preparing ethanol can reach 67%. Therefore, a production cost is relatively reduced.

The invention discloses a bagasse pretreatment method. The bagasse pretreatment method comprises the following steps of: drying, crushing and screening the bagasse, then pretreating the bagasse by using sodium hydroxide and hydrogen peroxide to obtain the pretreated bagasse. According to the pretreated bagasse material, the maximum removal rates of the hemicellulose and lignin respectively reach 78.18 percent and 94.56 percent, the maximum enzyme hydrolysis rate of the cellulose is 64.37 percent, and the enzymolysis efficiency is improved by 60.67 percent compared with that of untreated raw bagasse. Therefore, the pretreatment method breaks through wrapping of the hemicellulose and lignin to the cellulose, the hydrolysis rate of the cellulose is increased, the enzymolysis resistance property of the bagasse is improved, the obtained bagasse material can be used for converting high added value products, such as ethanol through microbial fermentation after being subjected to enzymolysis, and thus biomass resources, such as bagasse are effectively utilized.

The invention provides a method for performing lignocelluloses raw material high-temperature diluted acid pretreatment by using extremely small amount of water, which comprises the following steps: (1) pre-treating a lignocelluloses raw material to remove impurities; (2) mixing the raw material with 0.2 to 10 weight percent acidic solution in an extremely high solid to liquid ratio, uniformly stirring, and standing at normal temperature to infiltrate the acidic solution into the lignocelluloses raw material completely, wherein the extremely high solid to liquid ratio is 1:2-3:1; and (3) filling the obtained raw material into a pretreatment reactor at a 100 percent material filling rate, heating the raw material to a required temperature and pressure directly by high-temperature steam, keeping the temperature and pressure for a certain period of time, relieving pressure, taking the material out and cooling. When the method is used, water consumption and energy consumption are reduced greatly on the premise of ensuring the pretreatment effect, zero discharge of sewage is realized, a pretreated lignocelluloses raw material with high solid content is obtained, and the perfect connection with a subsequent high-solid-content biological processing process is realized.

The invention discloses beef marrow bone zymolyte and an application thereof in preparation of beef flavor. The beef marrow bone zymolyte is prepared by protease enzymolysis by taking beef bone mud as a raw material; the protease comprises neutral protease, flavourzyme and papain in the weight ratio of (3 to 5): (2 to 5): (2 to 5); the enzymolysis temperature is 45 to 55 DEG C; the enzymolysis time is 2 to 3 hours; and the beef bone mud is prepared by grinding the beef marrow bone. The beef flavor prepared from the beef marrow bone zymolyte disclosed by the invention through a Maillard reaction has unique beef marrow characteristic aroma; the fragrance is lasting and the flavor is unique; and flowing beef breath smell can be generated.

The present invention discloses enzyme extracting process of edible fungus leaching juice. The enzyme extracting process includes the following steps: mixing edible fungus in 24-34 wt% and water in 65-75 wt%, pulping, adding citric acid in 0.05-0.2 wt% and vitamin C in 0.02-0.1 wt%; heating at 60-80 deg.c to leach for 30-90 min before cooling to 45-55 deg.c, regulating pH value to 5.0-6.5 with 10 % concentration hydrochloric acid solution, adding compound cellulose in 0.2-1.0 wt% and enzymolysis at 45-55 deg.c for 90-150 min; adding compound protease in 0.2-1.0 water and enzymolysis at 45-55 deg.c for 60-120 min; fast heating to 90 deg.c, maintaining for 10 min to deactivate enzyme; and final pressure filtering and vacuum concentrating to obtain the edible fungus leaching juice.

The invention discloses a method for producing ethanol by high-efficiency simultaneous saccharification and cofermentation, which comprises the following steps: pretreating; concentrating and detoxifying; preparing yeast seed solution; performing simultaneous saccharification and cofermentation; and distilling/rectifying. When the method provided by the invention is used, the inhibition action of a monosaccharide component formed by hydrolysis of cellulose on cellulose is relieved, the enzymolysis saccharification efficiency and fermentation yield are improved, the recovery rate of hemicelluloses in bagasse is about 60 percent, the cellulose recovery rate is about 86 percent, and the total ethanol yield of dry bagasse can reach about 20 percent.

The invention discloses preparation and application of an immobilized protease reagent. An immobilized enzyme disclosed by the invention is composed of a hydrophobic carrier material and a protease immobilized on the hydrophobic carrier material. Two different-property, namely one hydrophilic and one hydrophobic, polymer chain modified magnetic nanoparticle immobilized trypsins prepared independently by using a surface initiate atom transfer radical polymerization (SI-ATRP) realize quick, efficient and complete enzymolysis of a protein, and the two, one hydrophilic and one hydrophobic, immobilized proteases are combined to use so that complementary enzymolysis is realized, and therefore, enzymatic bias caused by the selectivity of the carrier can be effectively reduced, the comprehensiveness of protein enzymolysis can be improved, and then the identification number of proteins and peptide fragments can be remarkably increased.

The invention discloses a process method for modified collaborative preparation of egg white powder through enzymolysis and phosphorylation. The process method sequentially comprises the steps of egg cleaning, egg white separation, gauze filtration, distilled water dilution, constant temperature enzymolysis, enzyme deactivation treatment, enzymatic hydrolysate centrifugal filtration, phosphorylation, TCA (Trichloroacetic Acid) precipitation and the like. According to the method, the egg white is used as a raw material; the egg white powder which is easy to absorb, has small fishy smell and has good solubility, foaminess and emulsibility is prepared through the combined mode of the enzymolysis and the phosphorylation. The processing characteristic of egg white protein and the utilization rate of the protein are improved.

The invention discloses a method for producing a seaweed protein feed through the synergistic effect of enzymolysis and fermentation. The method comprises the steps of fully mixing seaweed residue (the water content is 38%) and a feed enzyme preparation according to a certain proportion and performing the enzymolysis for 4-7h with the pH of 6.2-7.0 at the temperature being 35-45 DEG C to obtain a seaweed residue-feed enzyme preparation mixture; inoculating compound microorganisms and performing solid fermentation. The method has a simple process and is easy to operate; an external source is added to degrade a cellulose preparation to produce nutrition beneficial to microbial growth through the enzymolysis and make up the deficiency of enzyme produced by using the compound microorganisms, so as to stabilize the solid fermentation process and the product quality, greatly reduce the content of cellulose and increase the protein content; the seaweed protein feed is rich in multiple minerals, bioactive constituents and unknown somatomedin, and can be used as a protein feed for livestock and poultry and an aquatic feed; the method provides a technical support for growing tensions on developing novel protein resources and relieving a feed protein source.