68 results about "Hydroxyproline" patented technology

The invention discloses a preparation method of micromolecular active collagen peptide, which comprises the following steps: (1) cutting fresh leather into fragments, pretreating the leather with a sodium bicarbonate solution, rinsing the leather with deionized water, and refrigerating and storing; (2) mixing papain, bromelain, pancreatin, pepsase, alkaline protease, neutral protease and flavourzyme to obtain a compound enzyme preparation; (3) performing high-pressure steam sterilization on the leather at 110-140 kPa and 120-122 DEG C to obtain leather with the hydrolysis degree of 75-85%, then adding distilled water into the leather to obtain mucilage, then adding a compound enzyme preparation into the mucilage, reacting for 5.5-6.5 hours at the pH of 6.8-7.2 and the temperature of 50-55 DEG C, and then performing enzyme inactivation at the temperature of 85-95 DEG C; and (4) after the reaction system is cooled to room temperature, purifying and centrifuging the reaction system, and taking clarified supernate, thereby obtaining the product. Different animal skins are treated, the proportion of peptide fragments with the molecular weight smaller than 1000 Da of the prepared small-molecule active collagen peptide is larger than or equal to 80%, and the hydroxyproline content is larger than 3%.

The invention belongs to the technical field of nutritional compositions and cosmetics, and particularly relates to an anti-aging composition and application and a product thereof. The composition provided by the invention is composed of L-ergothioneine and alpha-ketoglutaric acid, or L-ergothioneine and alpha-ketoglutaric acid salt, or L-ergothioneine and alpha-ketoglutaric acid salt. Through the synergistic effect of the L-ergothioneine and the alpha-ketoglutaric acid or the alpha-ketoglutarate, the contents of Col I and hydroxyproline in skin tissues can be remarkably increased, the expression of TGF-beta1 is up-regulated and converted, the activity of MMP-1 is inhibited, meanwhile, the malondialdehyde level is reduced to enhance the oxidation resistance, the synthesis of hyaluronic acid is promoted, and the water content of the skin is increased. The composition is simple in component and simple and convenient in preparation process, can be prepared into various products such as cosmetics, health care products or medicines, is used for achieving the effects of resisting aging, moisturizing, resisting inflammation or resisting oxidation, particularly has an excellent improvement effect on skin photoaging induced by ultraviolet rays, and is wide in application prospect.

The invention relates to the technical field of collagen extraction, and provides a collagen extraction process for increasing hydroxyproline content, which sequentially comprises the steps of raw material pretreatment, deliming and degreasing, directional enzymolysis, gradient pressure-temperature synergistic extraction, purification, concentration and drying, a composite pretreatment agent containing a proline hydroxylase activator, EDTA-2Na and neutral protease is adopted in the raw material pretreatment; deashing and degreasing are combined with a chelating agent solution and ultrasonic treatment; a compound enzyme solution composed of trypsin, papain and proline hydroxylase is used for directional enzymolysis; a two-stage gradient pressure-temperature cooperation mode is adopted for extraction; purification is realized through combination of gel filtration chromatography and ion exchange chromatography; and finally carrying out freeze concentration and vacuum freeze drying to obtain the product. According to the process, through a multi-step synergistic effect, the content of hydroxyproline in collagen is remarkably increased, the product purity is high, natural functional characteristics are reserved, and the process is suitable for the fields of food, cosmetics, biological medicine and the like.

This invention belongs to the fields of genetic engineering and enzyme engineering, specifically relating to proline hydroxylase mutants and their application and methods in hydroxyproline production. This invention provides p4h... G141A p4h S85A p4h V126A p4h H125A p4h S205A p4h D122A p4h S211A Mutations at specific sites significantly increased the production of hydroxyproline and decreased the proportion of proline in the mutant enzyme. Further saturation mutations at these sites, combined with the dominant mutations, yielded strains with even better performance, such as p4h. S85Q‑V126A p4h S85A‑V126T p4h G141A‑H125S p4h G141A‑H125T Compared with traditional chemical methods, this process has lower production costs, is more environmentally friendly, and allows for large-scale continuous production.

The invention belongs to the technical field of biochemical engineering, and relates to an industrial extraction method and application of L-hydroxyproline adapted to the scale of a 30T fermentation tank, and the extraction method comprises the following steps: preparing raw materials; recycling membrane filtration and mother liquor; decoloring is performed; decarburization; evaporating, concentrating and crystallizing; performing centrifugal separation; drying and crushing. The method solves the technical problems that the yield is low (generally less than 70%), the process flow is long (multiple times of chromatography / recrystallization is needed), the product purity is difficult to reach the standard (most 98-99.5%) and the like, the L-hydroxyproline fermentation liquor produced by a 30T fermentation tank is used as a treatment object, efficient extraction is realized through a pretreatment-purification-refining three-stage process system, and the method is suitable for industrial production. According to the method, the primary mother liquor generated by centrifugal separation is innovatively applied to the next batch of membrane filtration process, so that raw material recycling is realized, the purity of the final product stably reaches 99.9%, the overall yield is increased to 75%, and the method has the advantages of simplified process, stable yield and controllable quality, and can be directly applied to large-scale industrial production of L-hydroxyproline.

This invention provides a highly water-soluble composition for promoting autophagy and its application. The composition comprises 5-hydroxyflavone, acetylhydroxyproline, and a suitable solvent. This composition is a unique vesicular composition that enables efficient delivery and synergistic effects of the two active ingredients. It promotes autophagy, has improved solubility, stability, and antioxidant properties, and can prevent and repair skin photodamage at its source. This invention also discloses a method for preparing this composition and its applications.

The invention discloses an application of a P4HA1 inhibitor in preparation of a medicine for treating congenital megacolon-related enterocolitis, deeply clarifies an immune metabolic axis of 'P4HA1-hydroxyproline-eIF5A-IRF4-IL17', and discloses a new mechanism that metabolite hydroxyproline regulates the activity of eIF5A and promotes nuclear translocation of IRF4 so as to enhance TH17 cell mediated inflammatory response. Therefore, a brand new perspective is provided for understanding metabolic immune regulation of intestinal inflammation. By using the P4HA1 inhibitor (such as 1, 4-DPCA), secretion of IL-17 can be effectively inhibited, intestinal inflammation injury of HAEC model mice can be remarkably relieved, and a clear pharmacodynamic basis is achieved.

The present application belongs to the technical field of nutritional functional food and health food, and particularly relates to a preparation method of marine fish skin collagen oligopeptide beneficial to promoting skin health, and a product and application thereof. The method comprises the following steps: after deep sea fish skin is pretreated by salt dissolution with a NaCl solution, trisodium citrate is added as an enzymolysis aid, and neutral protease (from Trichoderma reesei) and papain are sequentially used for two-step enzymolysis, and after enzyme inactivation, impurities are removed, sterilization is performed, and concentration, drying are performed to obtain the product. In vitro cell experiment verification shows that the product can significantly promote human skin fibroblast proliferation; in vivo animal experiment verification shows that the product can significantly increase skin moisture content, reduce transdermal water loss, increase hydroxyproline content, improve SOD activity and reduce MDA content. The product is suitable for the field of nutritional functional food and health food, and has a good application prospect in promoting skin health.

The present invention provides nutritional compositions for use in weight loss dietary interventions or in particular weight maintenance after dietary interventions, preferably after low calorie dietary interventions. The nutritional composition comprises one or more amino acids selected from the group consisting of proline, histidine, glycine and / or hydroxyproline, or a combination thereof, which can be used to assist in weight loss in obese subjects that are resistant to weight loss only by means of caloric limiting dietary intervention. In addition, the nutritional composition of the present invention may be used to help maintain the weight loss, particularly after dietary intervention.

The present application relates to the technical field of content detection, in particular to a method for rapidly detecting the content of hydroxyproline in mouse lung tissue, which comprises the following steps: obtaining the original sampling spectrum of the lung tissue of the mouse to be detected at different preset sampling points, and obtaining a non-lung tissue interference characteristic base matrix containing whole blood characteristics and cartilage characteristics; setting an orthogonal filter operator and determining a lung interstitium characteristic spectrum vector; constructing a reference spectrum vector and determining a relative spectral response factor; constructing a spectral minimum spanning tree and determining a signal contribution weight based on the spectral minimum spanning tree and the relative spectral response factor; generating a whole lung fibrosis synthetic spectrum according to the signal contribution weight and the lung interstitium characteristic spectrum vector, and detecting the content of hydroxyproline based on the whole lung fibrosis synthetic spectrum. The present application considers the non-lung tissue interference containing whole blood characteristics and cartilage characteristics when detecting the content of hydroxyproline, which improves the rationality of the detection of the content of hydroxyproline to a certain extent.

This invention provides the application of *Ackermania guangxiensis* extracellular polysaccharide in the preparation of anti-aging products, belonging to the technical field of microbial polysaccharide pharmaceutical applications. This invention extracts extracellular polysaccharides from *Ackermania guangxiensis* using the TCA precipitation method. Animal experiments show that *Ackermania guangxiensis* extracellular polysaccharide can effectively reduce MDA content in aging model mice while increasing SOD activity; it improves the thinning of the dermal layer, reduction and loosening of collagen fibers, and other signs of aging in model mice, and increases the hydroxyproline content in the skin; furthermore, it can activate the expression of BDNF and TrKB genes in the mouse hippocampus and alleviate problems such as slow movement, cognitive impairment, and memory decline in model mice. Therefore, the *Ackermania guangxiensis* extracellular polysaccharide provided by this invention has good anti-aging and antioxidant activity and can be used to prepare various anti-aging products.

Provided is a peptide compound comprising the amino acid sequence: Xaa1-Lys-Xaa3-Ser-Xaa5-Xaa6-Xaa7-Xaa8-Xaa9-Xaa10-Xaa11-Xaa12-Xaa13 wherein: Xaa1 represents Ala or Ser, or is absent, wherein the N-terminal amino acid optionally includes an N-terminal 3,4-dihydrocinnamic acid group; Xaa5 represents Tyr, DOPA or a single bond; Xaa3 and Xaa6 independently represent Pro, Hyp or diHyp; Xaa7, Xaa8, Xaa9, Xaa10, and Xaa11 are each optional (in which case Xaa6 is bonded to Xaa12), and are independently selected from the group Pro, Hyp, diHyp, Thr, DOPA and Tyr; Xaa12 represents Pro, Hyp, diHyp, Thr, DOPA or Tyr; and Xaa13 represents Lys or is absent, as well as regioisomers, stereoisomers, and pharmaceutically- or cosmetically-acceptable salts of said peptide compound, provided that when the N-terminal 3,4-dihydrocinnamic acid group is not present, Xaa1 represents Ala or is absent and Xaa13 represents Lys, then Xaa12 represents Pro, Hyp, diHyp or Thr. The compounds are particularly useful in for treatment of conditions characterised by inflammation, including wounds, burns, and disorders of the mucosa, such as anorectal diseases, inflammatory bowel diseases, gynaecological diseases and dental diseases.

The invention belongs to the technical field of cell therapy, and relates to a serum-free and heterologous component-free NK cell culture medium as well as a preparation method and application thereof. The culture medium comprises a classical culture medium, a proliferation promoting component, vitamins, trace elements, lipids, transferrin, an energy substance and beta-mercaptoethanol, the proliferation promoting component is prepared from human albumin, resveratrol, insulin and hydroxyproline. On the basis of a serum-free and heterologous component-free NK cell culture medium, IL-12, rapamycin, polyinosinic-polycytidylic acid and human AB plasma are additionally added to obtain an activated culture medium, the activated culture medium and an amplification culture medium are jointly used, the culture effect is better, and amplification components IL-2 and human AB plasma are additionally added to obtain an amplification culture medium. Through precise component design, the NK cell in-vitro proliferation efficiency, motility, purity and killing ability are remarkably improved, and key problems existing in a traditional culture medium are efficiently solved.

The invention relates to a moisturizing and wrinkle fading composition and application thereof. The moisturizing and wrinkle fading composition is prepared from the following components: beta-glucan, dipalmitoyl hydroxyproline, butyrospermum parkii fruit fat unsaponifiable matter, sodium hyaluronate and soluble collagen. The beta-glucan, the dipalmitoyl hydroxyproline, the butyrospermum parkii fruit fat unsaponifiable matter, the sodium hyaluronate and the soluble collagen are creatively combined, and the beta-glucan can activate the skin immune barrier and create a stable environment for repairing; sodium hyaluronate and butyrospermum parkii butter unsaponifiable substances can maintain skin moisture and reduce dry wrinkles; the dipalmitoyl hydroxyproline can stimulate collagen regeneration, and the soluble collagen provides a repairing raw material at the same time, so that the effects of long-acting moisturizing and deep wrinkle fading are achieved. The five functional components cooperate with one another and have a synergistic effect, the moisturizing and wrinkle fading effects are jointly achieved, and the problems that lips are dry and wrinkles are likely to be generated can be better solved.

The invention discloses application of gaboxadol or a pharmaceutically acceptable salt thereof in preparation of a medicine for treating liver fibrosis diseases, and belongs to the technical field of biological medicines, and research shows that the compound can influence collagen fiber crosslinking through targeted inhibition of the function of prolyl-4-hydroxylase a1 (prolyl-4-hydroxylase alpha 1, P4ha1), so that the anti-fibrosis effect is achieved. The invention further discloses an application of the gaboxadol or the pharmaceutically acceptable salt thereof in preparation of the medicine for treating the liver fibrosis diseases, and the application of the gaboxadol or the pharmaceutically acceptable salt thereof in preparation of the medicine for treating the liver fibrosis diseases and the application of the gaboxadol or the pharmaceutically acceptable salt thereof in preparation of the medicine for treating the liver fibrosis diseases. In a carbon tetrachloride induced mouse hepatic fibrosis model, the content of hydroxyproline and collagen deposition in I-type collagen molecules of the liver can be remarkably reduced by injecting the gaboxadol hydrochloride into the intraperitoneal cavity, and the hardness of the liver is reduced. A surface plasma resonance experiment proves that the probe has specific binding capacity with P4ha1. Toxicity experiments show that the compound is good in safety under the effective dosage. Therefore, the invention provides a new drug candidate and a theoretical basis for the treatment of hepatic fibrosis.

The objective is to provide a novel composition that promotes collagen production. [Solution] As a result of diligent research, the present inventors have found that a composition containing peony extract, rose extract, Bupleurum falcatum extract, and L-hydroxyproline promotes collagen production. Therefore, this disclosure provides a composition containing peony extract, rose extract, Bupleurum falcatum extract, and L-hydroxyproline.

The invention discloses an application of harpagoside in preparation of a medicine for preventing and / or treating hepatic fibrosis. The invention discloses an application of harpagoside in preparation of a health care product with an auxiliary protection effect on chemical liver injury. The animal level proves that the harpagoside can reduce hepatic fibrosis such as chemical hepatic fibrosis induced by carbon tetrachloride, reduce liver collagen deposition, reduce hydroxyproline content in hepatic tissue, reduce increased HA and Col IV content in serum, and reduce expression of fibrosis and collagen deposition related genes, so that the harpagoside can be used for treating hepatic fibrosis. The traditional Chinese medicine composition has a good effect of treating or preventing hepatic fibrosis.

Provided is a method for preparing a recombinant human collagen. The method comprises: constructing a genetically engineered bacterium which expresses a prolyl hydroxylase and a recombinant human collagen; culturing the genetically engineered bacterium and performing digestion treatment by means of pepsin to obtain a recombinant human collagen type I or type III. The prolyl hydroxylase comprises P4Ha2, P4Hb and vP4H, and by means of controlling the ratio of the copy numbers of the genes thereof, the recombinant human collagen and a natural human collagen are consistent in hydroxylation modification. Also provided is a large-scale production method for the recombinant human collagen. The prepared recombinant human collagen has completely the same sequence composition and length and substantially the same hydroxyproline content as the natural human collagen, and also has a stable full-length triple helix structure and biological activity without immunogenicity and potential risk of viruses.

The invention belongs to the technical field of biological material structure and performance analysis, and discloses a rapid qualitative and quantitative detection kit for a collagen triple-helix structure and a detection method. The detection kit comprises an enzymolysis system solution A of a non-triple helix structure of specific enzymolysis collagen; an ultrafiltration centrifugal tube; the enzymolysis system liquid B is used for performing non-specific enzymolysis on small-molecule polypeptide; colloidal gold test paper; the colloidal gold test paper is sequentially provided with a sample pad, a combination pad, an NC membrane and a water absorption pad from left to right; the NC membrane is sequentially coated with a T line and a C line from left to right; the marker on the combination pad is a gold-labeled antibody prepared from a hydroxyproline monoclonal antibody and colloidal gold particles through electrostatic coupling; and a coating substance on the T line is a hydroxyproline-carrier protein conjugate. The detection kit provided by the invention realizes a rapid, simple and good-specificity qualitative and quantitative detection method for the collagen triple-helix structure.

The application discloses a tripeptide with the effects of promoting HaCaT cell migration and barrier repair and application of the tripeptide, and the amino acid sequence of the tripeptide is glycine-methionine-hydroxyproline. The tripeptide has the effects of promoting HaCaT cell migration and barrier repair, and can be applied to the preparation of cosmetics with the effects of skin tissue repair and / or skin barrier repair.

The invention relates to the technical field of biological medicines, in particular to application of wild buckwheat rhizome freeze-dried powder in preparation of a medicine for preventing or treating antimony pneumoconiosis. A mouse antimony pneumoconiosis model is constructed by injecting an antimony (Sb) suspension into a trachea, and pulmonary fibrosis lesion caused by antimony exposure can be remarkably relieved by intragastric administration of the wild buckwheat rhizome freeze-dried powder prepared by the invention. Experimental results show that Hamp; e dyeing indicates that the freeze-dried powder can obviously relieve the fibrosis severity of the lung tissue; western Blot and PCR (Polymerase Chain Reaction) detection results show that the wild buckwheat rhizome can partially inhibit the expression up-regulation of Col-1 and alpha-SMA induced by antimony in protein and gene levels; in addition, the wild buckwheat rhizome can also obviously reduce the content of hydroxyproline in lung tissues. The results show that the wild buckwheat rhizome freeze-dried powder can effectively improve multiple pathological indexes and reduce the pulmonary fibrosis degree, has good potential in the aspect of preventing antimony pneumoconiosis, and has potential application value.

The invention belongs to the field of daily chemicals, and discloses an anti-wrinkle composition based on lipidosome delivery, which comprises the following components by weight: 0.1-1 part of a Chrysanthemum indicum extract; 0.1 to 1 part by weight of dipalmitoyl hydroxyproline; and 0.1 to 1 part by weight of methylsilanol mannuronate. According to the composition, dipalmitoyl hydroxyproline, a stellera chamaejasme extract and methylsilanol mannuronate serve as active ingredients, liposome serves as a carrier, and the anti-wrinkle effect can be achieved to the maximum extent. Meanwhile, the invention further discloses the lipidosome, application of the lipidosome and cosmetics.

The invention discloses an anti-aging drug screening method based on metabonomics. Firstly, a group of biomarkers, including L-valine, 4-hydroxyproline, L-serine, L-leucine, L-proline and melatonin, which are closely related to the caenorhabditis elegans aging process are identified in the invention. On the basis, the invention provides a drug screening method which comprises the following steps: performing metabonomics analysis on the nematodes subjected to drug administration treatment, detecting the content change of the biomarker group, and if the content of the biomarker group has obvious callback (up-regulation) compared with that of an old control group, judging that the drug has a potential anti-aging effect. The technical defects that a traditional nematode life experiment is long in period, tedious in operation, low in flux, high in subjectivity and the like are overcome, and a new anti-aging drug screening strategy which is rapid, stable and efficient and can preliminarily reveal drug effects is provided.

The invention relates to the technical field of metabonomics analysis, in particular to application of a metabolic marker in preparation of a head and neck squamous cell carcinoma diagnostic product, a kit, a screening method of the metabolic marker of the head and neck squamous cell carcinoma, a diagnostic model and a construction method and application of the diagnostic model. The metabolic marker comprises at least one of lactic acid, sphingosine, cadaverine, uridine diphosphate, allantoic acid, hydroxyproline, sphingosine-1-phosphoric acid, indole-3-acetaldehyde, pantothenic acid, fumaric acid, malic acid, prostaglandin or ornithine in red blood cells and / or plasma. The screened red blood cells and plasma metabolism markers can be used for predicting or diagnosing the head and neck squamous cell carcinoma respectively or independently, particularly, the red blood cell metabolism markers can provide more stable tumor microenvironment information, and the metabolism stability is high and is not easily influenced by diet and circadian rhythm.

The application belongs to the field of new daily chemical materials, and discloses a composition with skin efficacy improving function, application and cosmetic, the composition comprising acetyl hydroxyproline and white nymphaea alba extract; in addition, the application also discloses a whitening composition comprising ergothioneine, superoxide dismutase, acetyl hydroxyproline, white nymphaea alba extract and malt extract. The whitening composition realizes the whitening performance through double anti-glycation, double anti-oxidation, inhibition of the pathways of melanin and tyrosinase, and the composition also has potential anti-wrinkle and firming efficacy.

The invention relates to the technical field of litopenaeus vannamei feeds, in particular to an artificial compound feed for improving the muscle quality of freshwater cultured litopenaeus vannamei and a preparation method thereof.The artificial compound feed is prepared from, by weight, 18%-22% of fish meal, 33%-37% of soybean meal, 20%-24% of wheat flour, 1%-5% of squid powder, 2%-3% of fish oil, 1%-2% of soybean lecithin, 0.2%-1% of choline chloride and 0.1%-0.2% of calcium propionate; the feed additive is prepared from the following raw materials in percentage by weight: 0.05%-0.1% of an antioxidant, 0.2%-0.5% of sodium alginate, 1%-2% of monocalcium phosphate, 1%-2% of a multi-vitamin multi-mineral premix, 0.8% of a hydroxyproline and glutamine mixture and 10%-13% of microcrystalline cellulose. 0.8% by weight of a mixture of hydroxyproline and glutamine is added, so that the collagen content, hardness, water holding capacity and muscle fiber density in the muscle of the freshwater cultured prawns as well as the content of flavor amino acids (such as glycine, alanine and glutamic acid) and important umami substances IMP are remarkably improved; therefore, the muscle taste and flavor of the low-salt or freshwater cultured litopenaeus vannamei are improved.

1. Name of the designed product: packing box (Bama Hydroxycon). 2. Use of the designed product: carton for packing products. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo showing the design points: perspective view.

The invention provides a method for preparing recombinant I-type human collagen. The method comprises the following steps: carrying out fermentation culture treatment on engineering bacteria, wherein the engineering bacteria express precollagen; carrying out purification treatment on the fermentation culture treatment product so as to obtain the recombinant I-type human collagen; wherein the fermentation culture treatment is three-stage fermentation, and the final fermentation volume of the three-stage fermentation is not less than 9 tons. According to the method, the recombinant I-type human collagen with a complete triple helix structure, low immunogenicity, high safety and high purity can be prepared on a large scale, the recombinant I-type human collagen is composed of two alpha1 chains and one alpha2 chain, the hydroxyproline content is high, and the product stability is good.

The invention discloses application of demethylfolin or pharmaceutically acceptable salt thereof in preparation of a medicine for treating liver fibrosis diseases, belongs to the technical field of biological medicines, and discloses application of demethylfolin or pharmaceutically acceptable salt thereof in preparation of a medicine for treating liver fibrosis diseases through a mouse liver fibrosis model induced by carbon tetrachloride. It is proved that demethylfolin hydrochloride can significantly improve the pathological state of hepatic fibrosis: the content of hydroxyproline in liver type I collagen molecules is reduced, the liver tissue hardness is reduced, and the area of a fibrosis region is reduced. Toxicity experiments show that the compound has no obvious toxic or side effect on the liver in an effective dose and is good in safety. Therefore, the invention provides a new drug choice and theoretical basis for the treatment of hepatic fibrosis.

Provided herein are peptides obtained from hydrolyzed collagen and compositions comprising such. The peptides are from 9 to 100. or 19 to 50 amino acids in length. The peptides are unique from peptides obtained from enzymatically hydrolyzed collagen in that the peptides do not include hydroxyproline. Methods of making the hydroxyproline-free peptides obtained from hydrolyzed collagen, and methods of treatment using the hydroxyproline- free peptides obtained from hydrolyzed collagen are also provided.